MD03G1293400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011963
Physical Location & Seq
Forward (+)
37006700 .. 37007602
903 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1293400.v1.1.491

Sequence Viewer

Length: 903 bp
ATGGGTCGGCAGTACAATTCGGAACAAAGTGCTAAGAAGCTCTGGACCAATTCAACTATCTTCCTAAAACAACCTTTCCATATTCTCACACTCACCTTCCTCAGTTTATTGCTTCCCCTCTCATTCCTCCTCCTATCCAGGCTATCTTGCGCCAACTGTCTCTTAAGCTTAAGCTTCCCCACTCTCATCATTCCTCCTCCTGAGCCCTCCAACTACGATTCATCATGCATCTTCCATATCTTCCTTAATGCTGCTACTCCTTTTCTCTATCTTCTTGTTTCCATCGTCACCATAGCCGCCTTTATCCACTGCATAACAGGAAAAATCATCATCATAACCGAGTTGAGTCCCATTTTCCGTCCCCGTTTGAACACTGCTTGGATATTCCTGTGCACATTGCAAGTCTGTGTCGGTTTGGGAATCAAAGGTAGCATCGCCGCTGAGGTAGATGGGCGCGCTACTTTTGGCGGTGTTGTTGATGGCAAAATATGTTTGTCGAGCAGAAAAATATTTTTCCTAGGGTTGCATGAGACAATGCTTCATTGGTGTAGGGTTGTGGTGAAGCCGGTGATGGATGACACGATTTTCGGTGGCTCTAGAGAGGAAAAGTGGGTTGAGAGGGTGGCAATAGCTGCCGGCTTTGGTTGTTTGTGGTGGTGGAGGTTGAGGGACGAGGTTGAGTCTTTGGTTGTTGTGGCTATAGCTAAAAGAGAGCTGGCAATGGGCTTAGGAATGGCTGACTTTGTTGGTTGGTGGCTATATTACCTCACTGTGGCTATTGGTATGGTTAGGGTGGTAAAGGGTCTTATGTGGTTTTCCATGGTTTTGTTATGTAGAAGGACAGAAGCCAAACTTTGTAACGATTGCGGAGAAGATCATGGTCGTCAAGACAACAAGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

301

Amino Acids

33.71

Weight (kDa)

8.72

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 896
AccII CGCG 1 cut(s) 456
AciI CCGC 4 cut(s) 297, 438, 468, 867
AfaI GTAC 1 cut(s) 14
AfiI CCNNNNNNNGG 1 cut(s) 772
AflII CTTAAG 2 cut(s) 163, 169
AgsI TTSAA 2 cut(s) 54, 370
AjnI CCWGG 1 cut(s) 137
AluBI AGCT 6 cut(s) 40, 168, 174, 632, 704, 715
AluI AGCT 6 cut(s) 40, 168, 174, 632, 704, 715
Alw21I GWGCWC 1 cut(s) 395
Alw26I GTCTC 2 cut(s) 164, 524
Alw44I GTGCAC 1 cut(s) 391
ApaLI GTGCAC 1 cut(s) 391
ApeKI GCWGC 2 cut(s) 251, 632
ArsI GACNNNNNNTTYG 2 cut(s) 569, 601
AspA2I CCTAGG 1 cut(s) 517
AspLEI GCGC 3 cut(s) 152, 456, 458
AspS9I GGNCC 1 cut(s) 45
AsuHPI GGTGA 4 cut(s) 85, 280, 571, 580
AvaII GGWCC 1 cut(s) 45
AvrII CCTAGG 1 cut(s) 517
BaeGI GKGCMC 1 cut(s) 395
BanII GRGCYC 1 cut(s) 207
Bbv12I GWGCWC 1 cut(s) 395
BbvCI CCTCAGC 1 cut(s) 441
BbvI GCAGC 2 cut(s) 238, 619
BccI CCATC 4 cut(s) 290, 443, 473, 565
BciT130I CCWGG 1 cut(s) 139
BcoDI GTCTC 2 cut(s) 164, 524
BfaI CTAG 2 cut(s) 518, 597
BfmI CTRYAG 1 cut(s) 699
BfrI CTTAAG 2 cut(s) 163, 169
BisI GCNGC 4 cut(s) 252, 297, 438, 633
BlnI CCTAGG 1 cut(s) 517
BlsI GCNGC 4 cut(s) 253, 298, 439, 634
Bme1390I CCNGG 1 cut(s) 139
Bme18I GGWCC 1 cut(s) 45
BmgT120I GGNCC 1 cut(s) 45
BmrFI CCNGG 1 cut(s) 139
BmsI GCATC 2 cut(s) 237, 441
Bpu10I CCTNAGC 3 cut(s) 201, 441, 727
BsaJI CCNNGG 2 cut(s) 517, 819
Bsc4I CCNNNNNNNGG 1 cut(s) 772
Bse118I RCCGGY 2 cut(s) 565, 635
Bse3DI GCAATG 2 cut(s) 395, 726
BseBI CCWGG 1 cut(s) 139
BseDI CCNNGG 2 cut(s) 517, 819
BseGI GGATG 1 cut(s) 580
BseLI CCNNNNNNNGG 1 cut(s) 772
BseMI GCAATG 2 cut(s) 395, 726
BseMII CTCAG 3 cut(s) 115, 192, 432
BsePI GCGCGC 1 cut(s) 454
BseRI GAGGAG 2 cut(s) 119, 186
BseSI GKGCMC 1 cut(s) 395
BseXI GCAGC 2 cut(s) 238, 619
Bsh1236I CGCG 1 cut(s) 456
BsiHKAI GWGCWC 1 cut(s) 395
BsiSI CCGG 2 cut(s) 566, 636
BslFI GGGAC 3 cut(s) 333, 345, 683
BslI CCNNNNNNNGG 1 cut(s) 772
BsmAI GTCTC 2 cut(s) 164, 524
BsmFI GGGAC 3 cut(s) 333, 345, 683
Bsp1286I GDGCHC 2 cut(s) 207, 395
Bsp143I GATC 1 cut(s) 874
Bsp19I CCATGG 1 cut(s) 819
BspACI CCGC 4 cut(s) 297, 438, 468, 867
BspCNI CTCAG 3 cut(s) 114, 193, 433
BspFNI CGCG 1 cut(s) 456
BspTI CTTAAG 2 cut(s) 163, 169
BsrDI GCAATG 2 cut(s) 395, 726
BsrFI RCCGGY 2 cut(s) 565, 635
BssAI RCCGGY 2 cut(s) 565, 635
BssECI CCNNGG 2 cut(s) 517, 819
BssHII GCGCGC 1 cut(s) 454
BssMI GATC 1 cut(s) 874
BssT1I CCWWGG 2 cut(s) 517, 819
Bst2UI CCWGG 1 cut(s) 139
Bst4CI ACNGT 2 cut(s) 158, 772
BstAFI CTTAAG 2 cut(s) 163, 169
BstAPI GCANNNNNTGC 1 cut(s) 632
BstC8I GCNNGC 3 cut(s) 456, 637, 717
BstDEI CTNAG 5 cut(s) 33, 101, 201, 441, 727
BstDSI CCRYGG 1 cut(s) 819
BstF5I GGATG 1 cut(s) 580
BstFNI CGCG 1 cut(s) 456
BstHHI GCGC 3 cut(s) 152, 456, 458
BstKTI GATC 1 cut(s) 877
BstMAI GTCTC 2 cut(s) 164, 524
BstMBI GATC 1 cut(s) 874
BstMWI GCNNNNNNNGC 1 cut(s) 632
BstNI CCWGG 1 cut(s) 139
BstSCI CCNGG 1 cut(s) 137
BstSFI CTRYAG 1 cut(s) 699
BstSLI GKGCMC 1 cut(s) 395
BstUI CGCG 1 cut(s) 456
BstV1I GCAGC 2 cut(s) 238, 619
BtgI CCRYGG 1 cut(s) 819
BtgZI GCGATG 1 cut(s) 418
BtsCI GGATG 1 cut(s) 580
BtsI GCAGTG 2 cut(s) 307, 372
BtsIMutI CAGTG 3 cut(s) 307, 372, 768
Cac8I GCNNGC 3 cut(s) 456, 637, 717
CfoI GCGC 3 cut(s) 152, 456, 458
Cfr10I RCCGGY 2 cut(s) 565, 635
Cfr13I GGNCC 1 cut(s) 45
Csp6I GTAC 1 cut(s) 13
CviAII CATG 4 cut(s) 225, 527, 820, 878
CviQI GTAC 1 cut(s) 13
DdeI CTNAG 5 cut(s) 33, 101, 201, 441, 727
DpnI GATC 1 cut(s) 876
DpnII GATC 1 cut(s) 874
DrdI GACNNNNNNGTC 1 cut(s) 896
DseDI GACNNNNNNGTC 1 cut(s) 896
Eco130I CCWWGG 2 cut(s) 517, 819
Eco24I GRGCYC 1 cut(s) 207
Eco47I GGWCC 1 cut(s) 45
EcoRII CCWGG 1 cut(s) 137
EcoT14I CCWWGG 2 cut(s) 517, 819
EcoT22I ATGCAT 1 cut(s) 230
EcoT38I GRGCYC 1 cut(s) 207
ErhI CCWWGG 2 cut(s) 517, 819
FaeI CATG 4 cut(s) 228, 530, 823, 881
FalI AAGNNNNNCTT 2 cut(s) 837, 869
FaqI GGGAC 3 cut(s) 333, 345, 683
FatI CATG 4 cut(s) 224, 526, 819, 877
Fnu4HI GCNGC 4 cut(s) 252, 297, 438, 633
FokI GGATG 1 cut(s) 587
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 4 cut(s) 252, 297, 438, 633
FspBI CTAG 2 cut(s) 518, 597
GlaI GCGC 3 cut(s) 151, 455, 457
GluI GCNGC 4 cut(s) 252, 297, 438, 633
HapII CCGG 2 cut(s) 566, 636
HhaI GCGC 3 cut(s) 152, 456, 458
Hin1II CATG 4 cut(s) 228, 530, 823, 881
Hin6I GCGC 3 cut(s) 150, 454, 456
HinP1I GCGC 3 cut(s) 150, 454, 456
HindIII AAGCTT 2 cut(s) 166, 172
HinfI GANTC 4 cut(s) 218, 346, 420, 680
HpaII CCGG 2 cut(s) 566, 636
HphI GGTGA 4 cut(s) 85, 280, 571, 580
Hpy166II GTNNAC 1 cut(s) 393
Hpy188I TCNGA 1 cut(s) 22
Hpy188III TCNNGA 4 cut(s) 43, 200, 597, 887
Hpy8I GTNNAC 1 cut(s) 393
HpyAV CCTTC 2 cut(s) 106, 831
HpyCH4III ACNGT 2 cut(s) 158, 772
HpyCH4V TGCA 5 cut(s) 228, 312, 393, 400, 526
HpyF10VI GCNNNNNNNGC 1 cut(s) 632
HpyF3I CTNAG 5 cut(s) 33, 101, 201, 441, 727
Hsp92II CATG 4 cut(s) 228, 530, 823, 881
HspAI GCGC 3 cut(s) 150, 454, 456
KroI GCCGGC 1 cut(s) 635
KroNI GCCGGC 1 cut(s) 637
Kzo9I GATC 1 cut(s) 874
LpnPI CCDG 9 cut(s) 28, 124, 151, 213, 303, 401, 579, 649, 701
Lsp1109I GCAGC 2 cut(s) 238, 619
LweI GCATC 2 cut(s) 237, 441
MaeI CTAG 2 cut(s) 518, 597
MaeIII GTNAC 2 cut(s) 286, 857
MalI GATC 1 cut(s) 876
MboI GATC 1 cut(s) 874
MboII GAAGA 5 cut(s) 52, 223, 232, 263, 884
MhlI GDGCHC 2 cut(s) 207, 395
MluCI AATT 2 cut(s) 16, 49
MlyI GAGTC 2 cut(s) 355, 689
MmeI TCCRAC 1 cut(s) 234
Mph1103I ATGCAT 1 cut(s) 230
MroNI GCCGGC 1 cut(s) 635
MseI TTAA 3 cut(s) 164, 170, 246
MspA1I CMGCKG 1 cut(s) 440
MspCI CTTAAG 2 cut(s) 163, 169
MspI CCGG 2 cut(s) 566, 636
MspR9I CCNGG 1 cut(s) 139
MvaI CCWGG 1 cut(s) 139
MvnI CGCG 1 cut(s) 456
MwoI GCNNNNNNNGC 1 cut(s) 632
NaeI GCCGGC 1 cut(s) 637
NcoI CCATGG 1 cut(s) 819
NdeII GATC 1 cut(s) 874
NgoMIV GCCGGC 1 cut(s) 635
NlaIII CATG 4 cut(s) 228, 530, 823, 881
NmuCI GTSAC 1 cut(s) 286
NsiI ATGCAT 1 cut(s) 230
PauI GCGCGC 1 cut(s) 454
PdiI GCCGGC 1 cut(s) 637
PfeI GAWTC 2 cut(s) 218, 420
PkrI GCNGC 4 cut(s) 253, 298, 439, 634
PleI GAGTC 2 cut(s) 354, 688
PpsI GAGTC 2 cut(s) 354, 688
Psp6I CCWGG 1 cut(s) 137
PspGI CCWGG 1 cut(s) 137
PspPI GGNCC 1 cut(s) 45
PteI GCGCGC 1 cut(s) 454
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
SaqAI TTAA 3 cut(s) 164, 170, 246
SatI GCNGC 4 cut(s) 252, 297, 438, 633
Sau3AI GATC 1 cut(s) 874
Sau96I GGNCC 1 cut(s) 45
SchI GAGTC 2 cut(s) 355, 689
ScrFI CCNGG 1 cut(s) 139
SduI GDGCHC 2 cut(s) 207, 395
SfaNI GCATC 2 cut(s) 237, 441
SfcI CTRYAG 1 cut(s) 699
SinI GGWCC 1 cut(s) 45
SmlI CTYRAG 2 cut(s) 163, 169
SmoI CTYRAG 2 cut(s) 163, 169
Sse9I AATT 2 cut(s) 16, 49
SsiI CCGC 4 cut(s) 297, 438, 468, 867
SspI AATATT 1 cut(s) 510
SspMI CTAG 2 cut(s) 518, 597
StyD4I CCNGG 1 cut(s) 137
StyI CCWWGG 2 cut(s) 517, 819
TaaI ACNGT 2 cut(s) 158, 772
TaqI TCGA 1 cut(s) 497
TasI AATT 2 cut(s) 16, 49
TatI WGTACW 1 cut(s) 12
TauI GCSGC 2 cut(s) 299, 440
TfiI GAWTC 2 cut(s) 218, 420
Tru1I TTAA 3 cut(s) 164, 170, 246
Tru9I TTAA 3 cut(s) 164, 170, 246
TscAI CASTG 3 cut(s) 314, 379, 775
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 2 cut(s) 251, 632
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 2 cut(s) 210, 530
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 3 cut(s) 314, 379, 775
Vha464I CTTAAG 2 cut(s) 163, 169
VneI GTGCAC 1 cut(s) 391
VpaK11BI GGWCC 1 cut(s) 45
XbaI TCTAGA 1 cut(s) 596
XmaJI CCTAGG 1 cut(s) 517
XspI CTAG 2 cut(s) 518, 597
Zsp2I ATGCAT 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.