Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0010279

Physical Location & Seq

Genomic Coordinates Reverse (-)

37062306 .. 37064753

Gene Structure 2448 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1294400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1578 bp

ATGGAAAGGCAGAGAAGTTTCTCTTTCAAACCCACCAGATTCTTGGTGTTTTCTTTCACCATCTCTTCATCTCTAATCTTTCTCACTTTCTTTACCATCTGGCTTACTAAATCCACCCCTTCAATCCCCCAAGAAACCCACTTCCCGTCCGTAGCTATTCAGACTATAACCGGCTTCAGCGGAAATTTATCCGTAAATGCTCTGAAAGATTCGATCTTTGAAGGTGCCCATTTGAGTAAACCGGAAAATGCATCTGGGTTTTCTGGGGCTTCACTTACAGGTGATTTGCAGAAGAAGAAGAACGAATCCCAAGTTGCAGAACACGAAGAGGGAGGAGCAGGCGGTGGGCTAAATGGTAACTTCACATCGATGGAGAAAAGTGCTCCGGCAATCCTGTTTCTGAAGATTGAGAGTACTGAAGAGCAAAATGCTGAATTGAATAGGACTGGAGGGCAAAATGCTGAAGTAAATAGGACTGGAGGGCAAAATGCCGAATTGAATAGGACTGGAGGGCAAAATGCTGCACTGAAAAAGATTGAAATTTCAAGTGATGGAGTGGTTGAAGAGAAGAGAGTGAGAGGGTGTGATTTTACAAGAGGGAGGTGGGTATATGATGAGAGCTATCCTCTGTACACAAATGTGTCCTGTCCTTTGATAGATGAAGGTTTTAATTGTCAGGCCAATGGGAGAAAAGATAAAGACTACATGAAGTGGAGGTGGCAACCGCAAGATTGTGACATTCCAAGGTTCAATGCTACGAAAATGCTGGAATTGATGAGAGGGAAAAGACTAGTTTTCGTGGGGGATTCGATCAATAGGAATCAATGGGAATCCATGATGTGCATGTTGATGTCAGCTGTAAGAGATCCAACTAGGGTCTATGAGACGCATAGGCGTAGAATAACCAAAGAGAAGGGGAATTACAGTTTCAAATTTGTGGATTACAAGTGCACAGTTGAATATTATGCGTCCCATTTCTTGGTTCATGAAGGCAAGGGAAGAGTAGGTCAGAAGCGGGTGCAAACCTTGCGAATTGATTCCATCGATCATGGATCATCTAGATGGAGAGGAGCTAATATTTTGGTATTCAACACTGCTCATTGGTGGACCCATTACAAAACTAAAGCCGGGATTAATTACTACCAGGAAGCGGGCCAAATTCATCCGCAGCTTGATGTTTCCGCGGCTTTCAGAAAAGCTTTGATGACTTGGGGATCATGGGTTGATAAACACTTCAATCGCAAAACCCAAGTTTTCTTTCGAAGTTCAGCACCATCCCATTTCAGGGGAGGTCAATGGAATTCGGGTGGGAGTTGTAAGGAAGCAACCCAACCCCTCCAAACTTCGAGCTCTTCTTACTCTGAGAAGAATACAATTGCAGAAGAGGTCATAATGCAGATGAGAACTCCTGTGAAATTCTTGAACATAACCGGCTTGTCCGAATACAGAATCGATGGTCATCCGTCTATATATGGAAGAAATTCTAGAAAGTCGGGGATTCAAGACTGCAGTCATTGGTGTCTTCCTGGCATTCCTGATGCATGGAATGAATTGTTGTTTCTTCATTTGCTGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0008374 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071554

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

526

Amino Acids

59.56

Weight (kDa)

9.2

Isoelectric Point (pI)

41.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	194 - 246	4.4e-24	PMR5 N terminal Domain
PC-Esterase	PF13839	247 - 520	5.8e-98	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010279)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G62390 AT3G62390
fragaria_vesca	FvH4_5g39120 FvH4_5g39120 FvH4_5g39120
malus_domestica	MD03G1294400.v1.1
prunus_persica	Prupe.8G266500_v2.0.a1
pyrus_communis	pycom03g23280
rosa_chinensis	RchiOBHm_Chr4g0407051 RchiOBHm_Chr5g0073791 RchiOBHm_Chr7g0241111
rosa_laevigata	RLG00000000596
rosa_multiflora	Rmu_sc0003187.1_g000001
rosa_roxburghii	Rroxscaffold_3G00219260
rosa_rugosa	Rorug07G0342400 Rorug07G0342500
rosa_samantha	Rh7AG497600 Rh7BG468400 Rh7CG514500 Rh7DG481600
rosa_wichuraiana	Rw7G041980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	224
AccB7I	CCANNNNNTGG	1 cut(s)	979
AccII	CGCG	1 cut(s)	1184
AciI	CCGC	8 cut(s)	180, 342, 725, 1015, 1151, 1166, 1182, 1184
AclWI	GGATC	3 cut(s)	860, 1060, 1222
AcsI	RAATTY	7 cut(s)	184, 540, 932, 1158, 1300, 1415, 1480
AcuI	CTGAAG	4 cut(s)	160, 422, 438, 483
AfaI	GTAC	2 cut(s)	415, 632
AfiI	CCNNNNNNNGG	4 cut(s)	979, 1150, 1284, 1285
AhlI	ACTAGT	1 cut(s)	790
AjnI	CCWGG	2 cut(s)	1143, 1525
AjuI	GAANNNNNNNTTGG	2 cut(s)	1242, 1274
AleI	CACNNNNGTG	1 cut(s)	638
AluBI	AGCT	7 cut(s)	155, 621, 857, 1073, 1171, 1199, 1350
AluI	AGCT	7 cut(s)	155, 621, 857, 1073, 1171, 1199, 1350
Alw21I	GWGCWC	3 cut(s)	385, 953, 1352
Alw26I	GTCTC	1 cut(s)	878
Alw44I	GTGCAC	1 cut(s)	949
AlwI	GGATC	3 cut(s)	860, 1060, 1222
AoxI	GGCC	2 cut(s)	678, 1153
ApaLI	GTGCAC	1 cut(s)	949
ApeKI	GCWGC	3 cut(s)	521, 1168, 1570
ApoI	RAATTY	7 cut(s)	184, 540, 932, 1158, 1300, 1415, 1480
AseI	ATTAAT	1 cut(s)	1134
Asp700I	GAANNNNTTC	2 cut(s)	1036, 1480
AspS9I	GGNCC	2 cut(s)	1107, 1153
AsuC2I	CCSGG	1 cut(s)	1129
AsuHPI	GGTGA	2 cut(s)	49, 293
AsuII	TTCGAA	1 cut(s)	1261
AvaII	GGWCC	1 cut(s)	1107
BaeGI	GKGCMC	2 cut(s)	229, 953
BanI	GGYRCC	1 cut(s)	224
BanII	GRGCYC	1 cut(s)	1352
BarI	GAAGNNNNNNTAC	2 cut(s)	905, 937
BbsI	GAAGAC	1 cut(s)	1514
Bbv12I	GWGCWC	3 cut(s)	385, 953, 1352
BbvI	GCAGC	3 cut(s)	508, 1180, 1557
BccI	CCATC	8 cut(s)	68, 104, 364, 545, 1049, 1056, 1282, 1448
BciT130I	CCWGG	2 cut(s)	1145, 1527
BcnI	CCSGG	1 cut(s)	1129
BcoDI	GTCTC	1 cut(s)	878
BcuI	ACTAGT	1 cut(s)	790
BfaI	CTAG	4 cut(s)	791, 873, 1059, 1485
BfmI	CTRYAG	2 cut(s)	1507, 1571
BisI	GCNGC	4 cut(s)	522, 1169, 1185, 1571
BlsI	GCNGC	4 cut(s)	523, 1170, 1186, 1572
BmcAI	AGTACT	1 cut(s)	415
Bme1390I	CCNGG	3 cut(s)	1129, 1145, 1527
Bme18I	GGWCC	1 cut(s)	1107
BmgT120I	GGNCC	2 cut(s)	1107, 1153
BmiI	GGNNCC	2 cut(s)	226, 1109
BmrFI	CCNGG	3 cut(s)	1129, 1145, 1527
BmsI	GCATC	2 cut(s)	260, 1528
BoxI	GACNNNNGTC	1 cut(s)	1509
BpiI	GAAGAC	1 cut(s)	1514
BplI	GAGNNNNNCTC	2 cut(s)	610, 642
BpmI	CTGGAG	3 cut(s)	468, 498, 528
Bpu14I	TTCGAA	1 cut(s)	1261
BpuMI	CCSGG	1 cut(s)	1129
Bsa29I	ATCGAT	3 cut(s)	368, 1044, 1452
BsaBI	GATNNNNATC	1 cut(s)	1458
BsaJI	CCNNGG	2 cut(s)	743, 1182
BsaWI	WCCGGW	1 cut(s)	241
Bsc4I	CCNNNNNNNGG	4 cut(s)	979, 1150, 1284, 1285
Bse118I	RCCGGY	2 cut(s)	170, 1430
Bse1I	ACTGG	3 cut(s)	451, 481, 511
Bse8I	GATNNNNATC	1 cut(s)	1458
BseBI	CCWGG	2 cut(s)	1145, 1527
BseCI	ATCGAT	3 cut(s)	368, 1044, 1452
BseDI	CCNNGG	2 cut(s)	743, 1182
BseGI	GGATG	3 cut(s)	1162, 1274, 1459
BseJI	GATNNNNATC	1 cut(s)	1458
BseLI	CCNNNNNNNGG	4 cut(s)	979, 1150, 1284, 1285
BseMII	CTCAG	1 cut(s)	1353
BseNI	ACTGG	3 cut(s)	451, 481, 511
BseRI	GAGGAG	2 cut(s)	348, 1083
BseSI	GKGCMC	2 cut(s)	229, 953
BseXI	GCAGC	3 cut(s)	508, 1180, 1557
BsgI	GTGCAG	1 cut(s)	507
Bsh1236I	CGCG	1 cut(s)	1184
BshFI	GGCC	2 cut(s)	680, 1155
BshNI	GGYRCC	1 cut(s)	224
BshVI	ATCGAT	3 cut(s)	368, 1044, 1452
BsiHKAI	GWGCWC	3 cut(s)	385, 953, 1352
BsiSI	CCGG	5 cut(s)	171, 242, 386, 1128, 1431
BslFI	GGGAC	1 cut(s)	955
BslI	CCNNNNNNNGG	4 cut(s)	979, 1150, 1284, 1285
BsmAI	GTCTC	1 cut(s)	878
BsmBI	CGTCTC	1 cut(s)	878
BsmFI	GGGAC	1 cut(s)	955
BsmI	GAATGC	1 cut(s)	1530
BsnI	GGCC	2 cut(s)	680, 1155
Bsp119I	TTCGAA	1 cut(s)	1261
Bsp1286I	GDGCHC	4 cut(s)	229, 385, 953, 1352
Bsp1407I	TGTACA	1 cut(s)	630
Bsp143I	GATC	6 cut(s)	213, 810, 865, 1045, 1052, 1214
BspACI	CCGC	8 cut(s)	180, 342, 725, 1015, 1151, 1166, 1182, 1184
BspANI	GGCC	2 cut(s)	680, 1155
BspCNI	CTCAG	1 cut(s)	1354
BspDI	ATCGAT	3 cut(s)	368, 1044, 1452
BspFNI	CGCG	1 cut(s)	1184
BspHI	TCATGA	1 cut(s)	985
BspLI	GGNNCC	2 cut(s)	226, 1109
BspMAI	CTGCAG	2 cut(s)	1511, 1575
BspPI	GGATC	3 cut(s)	860, 1060, 1222
BspQI	GCTCTTC	2 cut(s)	414, 1357
BspT104I	TTCGAA	1 cut(s)	1261
BspT107I	GGYRCC	1 cut(s)	224
BsrFI	RCCGGY	2 cut(s)	170, 1430
BsrGI	TGTACA	1 cut(s)	630
BsrI	ACTGG	3 cut(s)	451, 481, 511
BssAI	RCCGGY	2 cut(s)	170, 1430
BssECI	CCNNGG	2 cut(s)	743, 1182
BssMI	GATC	6 cut(s)	213, 810, 865, 1045, 1052, 1214
BssT1I	CCWWGG	1 cut(s)	743
Bst2UI	CCWGG	2 cut(s)	1145, 1527
Bst4CI	ACNGT	2 cut(s)	926, 955
Bst6I	CTCTTC	8 cut(s)	70, 321, 414, 558, 563, 994, 1357, 1377
BstAPI	GCANNNNNTGC	1 cut(s)	1027
BstAUI	TGTACA	1 cut(s)	630
BstBI	TTCGAA	1 cut(s)	1261
BstC8I	GCNNGC	2 cut(s)	340, 1153
BstDEI	CTNAG	1 cut(s)	1362
BstDSI	CCRYGG	1 cut(s)	1182
BstF5I	GGATG	3 cut(s)	1162, 1274, 1459
BstFNI	CGCG	1 cut(s)	1184
BstKTI	GATC	6 cut(s)	216, 813, 868, 1048, 1055, 1217
BstMAI	GTCTC	1 cut(s)	878
BstMBI	GATC	6 cut(s)	213, 810, 865, 1045, 1052, 1214
BstMWI	GCNNNNNNNGC	1 cut(s)	1027
BstNI	CCWGG	2 cut(s)	1145, 1527
BstNSI	RCATGY	1 cut(s)	847
BstPAI	GACNNNNGTC	1 cut(s)	1509
BstSCI	CCNGG	3 cut(s)	1127, 1143, 1525
BstSFI	CTRYAG	2 cut(s)	1507, 1571
BstSLI	GKGCMC	2 cut(s)	229, 953
BstUI	CGCG	1 cut(s)	1184
BstV1I	GCAGC	3 cut(s)	508, 1180, 1557
BstV2I	GAAGAC	1 cut(s)	1514
BstX2I	RGATCY	1 cut(s)	865
BstXI	CCANNNNNNTGG	1 cut(s)	43
BstYI	RGATCY	1 cut(s)	865
Bsu15I	ATCGAT	3 cut(s)	368, 1044, 1452
BsuRI	GGCC	2 cut(s)	680, 1155
BsuTUI	ATCGAT	3 cut(s)	368, 1044, 1452
BtgI	CCRYGG	1 cut(s)	1182
BtsCI	GGATG	3 cut(s)	1162, 1274, 1459
BtsI	GCAGTG	1 cut(s)	1092
BtsIMutI	CAGTG	2 cut(s)	524, 1092
Cac8I	GCNNGC	2 cut(s)	340, 1153
CciI	TCATGA	1 cut(s)	985
Cfr10I	RCCGGY	2 cut(s)	170, 1430
Cfr13I	GGNCC	2 cut(s)	1107, 1153
Cfr42I	CCGCGG	1 cut(s)	1185
ClaI	ATCGAT	3 cut(s)	368, 1044, 1452
CseI	GACGC	2 cut(s)	895, 957
Csp6I	GTAC	2 cut(s)	414, 631
CviAII	CATG	7 cut(s)	706, 835, 844, 986, 1049, 1218, 1542
CviQI	GTAC	2 cut(s)	414, 631
DdeI	CTNAG	1 cut(s)	1362
DpnI	GATC	6 cut(s)	215, 812, 867, 1047, 1054, 1216
DpnII	GATC	6 cut(s)	213, 810, 865, 1045, 1052, 1214
Eam1104I	CTCTTC	8 cut(s)	70, 321, 414, 558, 563, 994, 1357, 1377
EarI	CTCTTC	8 cut(s)	70, 321, 414, 558, 563, 994, 1357, 1377
Ecl136II	GAGCTC	1 cut(s)	1350
Eco130I	CCWWGG	1 cut(s)	743
Eco24I	GRGCYC	1 cut(s)	1352
Eco47I	GGWCC	1 cut(s)	1107
Eco53kI	GAGCTC	1 cut(s)	1350
Eco57I	CTGAAG	4 cut(s)	160, 422, 438, 483
EcoICRI	GAGCTC	1 cut(s)	1350
EcoRI	GAATTC	1 cut(s)	1300
EcoRII	CCWGG	2 cut(s)	1143, 1525
EcoT14I	CCWWGG	1 cut(s)	743
EcoT22I	ATGCAT	2 cut(s)	253, 1543
EcoT38I	GRGCYC	1 cut(s)	1352
ErhI	CCWWGG	1 cut(s)	743
Esp3I	CGTCTC	1 cut(s)	878
FaeI	CATG	7 cut(s)	709, 838, 847, 989, 1052, 1221, 1545
FalI	AAGNNNNNCTT	2 cut(s)	7, 39
FaqI	GGGAC	1 cut(s)	955
FatI	CATG	7 cut(s)	705, 834, 843, 985, 1048, 1217, 1541
FauI	CCCGC	2 cut(s)	1008, 1144
Fnu4HI	GCNGC	4 cut(s)	522, 1169, 1185, 1571
FokI	GGATG	3 cut(s)	1149, 1261, 1446
FriOI	GRGCYC	1 cut(s)	1352
Fsp4HI	GCNGC	4 cut(s)	522, 1169, 1185, 1571
FspBI	CTAG	4 cut(s)	791, 873, 1059, 1485
GluI	GCNGC	4 cut(s)	522, 1169, 1185, 1571
GsuI	CTGGAG	3 cut(s)	468, 498, 528
HaeIII	GGCC	2 cut(s)	680, 1155
HapII	CCGG	5 cut(s)	171, 242, 386, 1128, 1431
HgaI	GACGC	2 cut(s)	895, 957
Hin1II	CATG	7 cut(s)	709, 838, 847, 989, 1052, 1221, 1545
HindIII	AAGCTT	1 cut(s)	1197
HinfI	GANTC	9 cut(s)	39, 209, 305, 806, 820, 830, 1037, 1449, 1498
HpaII	CCGG	5 cut(s)	171, 242, 386, 1128, 1431
HphI	GGTGA	2 cut(s)	49, 293
Hpy166II	GTNNAC	4 cut(s)	239, 633, 951, 1107
Hpy188I	TCNGA	7 cut(s)	162, 204, 402, 1011, 1193, 1363, 1441
Hpy188III	TCNNGA	6 cut(s)	986, 1059, 1420, 1485, 1502, 1535
Hpy8I	GTNNAC	4 cut(s)	239, 633, 951, 1107
HpyAV	CCTTC	5 cut(s)	129, 215, 656, 907, 983
HpyCH4III	ACNGT	2 cut(s)	926, 955
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1027
HpyF3I	CTNAG	1 cut(s)	1362
Hsp92II	CATG	7 cut(s)	709, 838, 847, 989, 1052, 1221, 1545
KspI	CCGCGG	1 cut(s)	1185
Kzo9I	GATC	6 cut(s)	213, 810, 865, 1045, 1052, 1214
LguI	GCTCTTC	2 cut(s)	414, 1357
LmnI	GCTCC	3 cut(s)	335, 388, 1070
Lsp1109I	GCAGC	3 cut(s)	508, 1180, 1557
LweI	GCATC	2 cut(s)	260, 1528
MaeI	CTAG	4 cut(s)	791, 873, 1059, 1485
MaeIII	GTNAC	2 cut(s)	356, 734
MalI	GATC	6 cut(s)	215, 812, 867, 1047, 1054, 1216
MboI	GATC	6 cut(s)	213, 810, 865, 1045, 1052, 1214
MfeI	CAATTG	1 cut(s)	1374
MflI	RGATCY	1 cut(s)	865
MhlI	GDGCHC	4 cut(s)	229, 385, 953, 1352
MmeI	TCCRAC	1 cut(s)	893
Mph1103I	ATGCAT	2 cut(s)	253, 1543
MroXI	GAANNNNTTC	2 cut(s)	1036, 1480
MseI	TTAA	2 cut(s)	669, 1134
MslI	CAYNNNNRTG	4 cut(s)	368, 638, 848, 1060
MspA1I	CMGCKG	3 cut(s)	180, 857, 1184
MspI	CCGG	5 cut(s)	171, 242, 386, 1128, 1431
MspR9I	CCNGG	3 cut(s)	1129, 1145, 1527
MunI	CAATTG	1 cut(s)	1374
Mva1269I	GAATGC	1 cut(s)	1530
MvaI	CCWGG	2 cut(s)	1145, 1527
MvnI	CGCG	1 cut(s)	1184
MwoI	GCNNNNNNNGC	1 cut(s)	1027
NciI	CCSGG	1 cut(s)	1129
NdeII	GATC	6 cut(s)	213, 810, 865, 1045, 1052, 1214
NlaIII	CATG	7 cut(s)	709, 838, 847, 989, 1052, 1221, 1545
NlaIV	GGNNCC	2 cut(s)	226, 1109
NmuCI	GTSAC	1 cut(s)	734
NsiI	ATGCAT	2 cut(s)	253, 1543
NspI	RCATGY	1 cut(s)	847
NspV	TTCGAA	1 cut(s)	1261
OliI	CACNNNNGTG	1 cut(s)	638
PagI	TCATGA	1 cut(s)	985
PciSI	GCTCTTC	2 cut(s)	414, 1357
PctI	GAATGC	1 cut(s)	1530
PdmI	GAANNNNTTC	2 cut(s)	1036, 1480
PfeI	GAWTC	9 cut(s)	39, 209, 305, 806, 820, 830, 1037, 1449, 1498
PflMI	CCANNNNNTGG	1 cut(s)	979
PkrI	GCNGC	4 cut(s)	523, 1170, 1186, 1572
PshAI	GACNNNNGTC	1 cut(s)	1509
PshBI	ATTAAT	1 cut(s)	1134
Psp124BI	GAGCTC	1 cut(s)	1352
Psp6I	CCWGG	2 cut(s)	1143, 1525
PspGI	CCWGG	2 cut(s)	1143, 1525
PspN4I	GGNNCC	2 cut(s)	226, 1109
PspPI	GGNCC	2 cut(s)	1107, 1153
PstI	CTGCAG	2 cut(s)	1511, 1575
PsuI	RGATCY	1 cut(s)	865
PvuII	CAGCTG	1 cut(s)	857
RsaI	GTAC	2 cut(s)	415, 632
RsaNI	GTAC	2 cut(s)	414, 631
RseI	CAYNNNNRTG	4 cut(s)	368, 638, 848, 1060
SacI	GAGCTC	1 cut(s)	1352
SacII	CCGCGG	1 cut(s)	1185
SapI	GCTCTTC	2 cut(s)	414, 1357
SaqAI	TTAA	2 cut(s)	669, 1134
SatI	GCNGC	4 cut(s)	522, 1169, 1185, 1571
Sau3AI	GATC	6 cut(s)	213, 810, 865, 1045, 1052, 1214
Sau96I	GGNCC	2 cut(s)	1107, 1153
ScaI	AGTACT	1 cut(s)	415
ScrFI	CCNGG	3 cut(s)	1129, 1145, 1527
SduI	GDGCHC	4 cut(s)	229, 385, 953, 1352
SfaNI	GCATC	2 cut(s)	260, 1528
SfcI	CTRYAG	2 cut(s)	1507, 1571
Sfr303I	CCGCGG	1 cut(s)	1185
SfuI	TTCGAA	1 cut(s)	1261
SgrBI	CCGCGG	1 cut(s)	1185
SinI	GGWCC	1 cut(s)	1107
SmiMI	CAYNNNNRTG	4 cut(s)	368, 638, 848, 1060
SpeI	ACTAGT	1 cut(s)	790
SsiI	CCGC	8 cut(s)	180, 342, 725, 1015, 1151, 1166, 1182, 1184
SspI	AATATT	2 cut(s)	962, 1078
SspMI	CTAG	4 cut(s)	791, 873, 1059, 1485
SstI	GAGCTC	1 cut(s)	1352
StyD4I	CCNGG	3 cut(s)	1127, 1143, 1525
StyI	CCWWGG	1 cut(s)	743
TaaI	ACNGT	2 cut(s)	926, 955
TaqI	TCGA	7 cut(s)	212, 368, 809, 1044, 1261, 1346, 1452
TatI	WGTACW	2 cut(s)	413, 630
TauI	GCSGC	1 cut(s)	1187
TfiI	GAWTC	9 cut(s)	39, 209, 305, 806, 820, 830, 1037, 1449, 1498
Tru1I	TTAA	2 cut(s)	669, 1134
Tru9I	TTAA	2 cut(s)	669, 1134
TscAI	CASTG	2 cut(s)	531, 1099
TseFI	GTSAC	1 cut(s)	734
TseI	GCWGC	3 cut(s)	521, 1168, 1570
Tsp45I	GTSAC	1 cut(s)	734
TspDTI	ATGAA	8 cut(s)	57, 675, 722, 974, 1002, 1151, 1553, 1563
TspGWI	ACGGA	3 cut(s)	139, 181, 1452
TspRI	CASTG	2 cut(s)	531, 1099
Van91I	CCANNNNNTGG	1 cut(s)	979
VneI	GTGCAC	1 cut(s)	949
VpaK11BI	GGWCC	1 cut(s)	1107
VspI	ATTAAT	1 cut(s)	1134
XapI	RAATTY	7 cut(s)	184, 540, 932, 1158, 1300, 1415, 1480
XbaI	TCTAGA	2 cut(s)	1058, 1484
XceI	RCATGY	1 cut(s)	847
XcmI	CCANNNNNNNNNTGG	1 cut(s)	40
XmnI	GAANNNNTTC	2 cut(s)	1036, 1480
XspI	CTAG	4 cut(s)	791, 873, 1059, 1485
ZrmI	AGTACT	1 cut(s)	415
Zsp2I	ATGCAT	2 cut(s)	253, 1543

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.