Rorug07G0342500

Protein trichome birefringence-like 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0010279
Physical Location & Seq
Forward (+)
37734612 .. 37735157
546 bp
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UTR
Exon/CDS
Intron
Rorug07G0342500.1

Sequence Viewer

Length: 546 bp
ATGAAGTGTTATGGTGCGGCCTCTGATAGCTCAGGTCGTTATGGTTTGGGCTTTGTTGTGAGAAATGAGGTGGGAGATGTCTTGTTGGCTGCGGGGCAAAGAATTGTTAATCCAGTTTCAGCTTTGGTCTCGGAATTGTTAGCCATCAAAGAAGGGTTGGAAACGGTGAAGGAAAGAAGCTTGAATAATGTTGAGGTGGAGACTGATTGCTTAAATGCAGTGCAGCTTATTAATCATAAGGAGAAATGTTTTGCGGCGGAAGGTATTATAGTGGAAGAGATTCAGATTCTTCTAGGAGAGCTTGGAATTTCAGTGATCAAGTATACGTCGCGGAATAATAACAAGGATGCTCATGCAGTTGCAGATTTTGTAGCCCGAGAGGATGGGCGTATATTATGGTTAGAGGATGTGCCTTCTTGGCTCATGTCCATCACCGAGAGTGAGAGGCCTCTAACGCAAGTAGTTGGCACGGAAATGAGTAGTTCTGGTTTTCCCAGGTTGTTGGGAGATACTATGGCTACACCGGATCATTCCCGAATTATGTAA

Protein Analysis

181

Amino Acids

19.76

Weight (kDa)

4.84

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 5 - 122 2.8e-17 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 323
AccII CGCG 1 cut(s) 331
AciI CCGC 5 cut(s) 17, 92, 254, 257, 331
AclWI GGATC 1 cut(s) 534
AcsI RAATTY 1 cut(s) 306
AgsI TTSAA 1 cut(s) 184
AjnI CCWGG 1 cut(s) 494
AluBI AGCT 5 cut(s) 30, 122, 180, 226, 301
AluI AGCT 5 cut(s) 30, 122, 180, 226, 301
Alw26I GTCTC 2 cut(s) 133, 194
AlwI GGATC 1 cut(s) 534
Ama87I CYCGRG 1 cut(s) 375
AoxI GGCC 2 cut(s) 18, 446
ApeKI GCWGC 2 cut(s) 89, 223
ApoI RAATTY 1 cut(s) 306
AseI ATTAAT 1 cut(s) 231
Asp700I GAANNNNTTC 1 cut(s) 279
AsuHPI GGTGA 2 cut(s) 178, 424
AvaI CYCGRG 1 cut(s) 375
BbvI GCAGC 2 cut(s) 76, 235
BccI CCATC 3 cut(s) 152, 377, 437
BciT130I CCWGG 1 cut(s) 496
BclI TGATCA 1 cut(s) 315
BcoDI GTCTC 2 cut(s) 133, 194
BfaI CTAG 1 cut(s) 293
BglI GCCNNNNNGGC 1 cut(s) 418
BisI GCNGC 4 cut(s) 18, 90, 224, 255
BlsI GCNGC 4 cut(s) 19, 91, 225, 256
Bme1390I CCNGG 1 cut(s) 496
BmeT110I CYCGRG 1 cut(s) 375
BmrFI CCNGG 1 cut(s) 496
BmsI GCATC 1 cut(s) 337
Bpu10I CCTNAGC 1 cut(s) 31
BsaI GGTCTC 1 cut(s) 133
BsaJI CCNNGG 1 cut(s) 494
BsaWI WCCGGW 1 cut(s) 523
Bse1I ACTGG 1 cut(s) 113
BseBI CCWGG 1 cut(s) 496
BseDI CCNNGG 1 cut(s) 494
BseGI GGATG 3 cut(s) 352, 388, 412
BseMII CTCAG 1 cut(s) 45
BseNI ACTGG 1 cut(s) 113
BseXI GCAGC 2 cut(s) 76, 235
BsgI GTGCAG 1 cut(s) 242
Bsh1236I CGCG 1 cut(s) 331
BshFI GGCC 2 cut(s) 20, 448
BsiHKCI CYCGRG 1 cut(s) 375
BsiSI CCGG 1 cut(s) 524
BsmAI GTCTC 2 cut(s) 133, 194
BsnI GGCC 2 cut(s) 20, 448
Bso31I GGTCTC 1 cut(s) 133
BsoBI CYCGRG 1 cut(s) 375
Bsp143I GATC 2 cut(s) 315, 526
BspACI CCGC 5 cut(s) 17, 92, 254, 257, 331
BspANI GGCC 2 cut(s) 20, 448
BspCNI CTCAG 1 cut(s) 44
BspFNI CGCG 1 cut(s) 331
BspPI GGATC 1 cut(s) 534
BspTNI GGTCTC 1 cut(s) 133
BsrI ACTGG 1 cut(s) 113
BssECI CCNNGG 1 cut(s) 494
BssMI GATC 2 cut(s) 315, 526
BssNAI GTATAC 1 cut(s) 324
Bst1107I GTATAC 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 496
Bst4CI ACNGT 1 cut(s) 166
Bst6I CTCTTC 1 cut(s) 270
BstDEI CTNAG 1 cut(s) 31
BstF5I GGATG 3 cut(s) 352, 388, 412
BstFNI CGCG 1 cut(s) 331
BstKTI GATC 2 cut(s) 318, 529
BstMAI GTCTC 2 cut(s) 133, 194
BstMBI GATC 2 cut(s) 315, 526
BstMWI GCNNNNNNNGC 2 cut(s) 418, 454
BstNI CCWGG 1 cut(s) 496
BstSCI CCNGG 1 cut(s) 494
BstUI CGCG 1 cut(s) 331
BstV1I GCAGC 2 cut(s) 76, 235
BstXI CCANNNNNNTGG 1 cut(s) 502
BstZ17I GTATAC 1 cut(s) 324
BsuRI GGCC 2 cut(s) 20, 448
BtsCI GGATG 3 cut(s) 352, 388, 412
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 2 cut(s) 225, 318
CviAII CATG 2 cut(s) 353, 424
DdeI CTNAG 1 cut(s) 31
DpnI GATC 2 cut(s) 317, 528
DpnII GATC 2 cut(s) 315, 526
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
EciI GGCGGA 1 cut(s) 272
Eco147I AGGCCT 1 cut(s) 448
Eco31I GGTCTC 1 cut(s) 133
Eco88I CYCGRG 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 494
FaeI CATG 2 cut(s) 356, 427
FatI CATG 2 cut(s) 352, 423
FauI CCCGC 1 cut(s) 85
FbaI TGATCA 1 cut(s) 315
FblI GTMKAC 1 cut(s) 323
Fnu4HI GCNGC 4 cut(s) 18, 90, 224, 255
FokI GGATG 3 cut(s) 359, 395, 419
Fsp4HI GCNGC 4 cut(s) 18, 90, 224, 255
FspBI CTAG 1 cut(s) 293
GluI GCNGC 4 cut(s) 18, 90, 224, 255
HaeIII GGCC 2 cut(s) 20, 448
HapII CCGG 1 cut(s) 524
Hin1II CATG 2 cut(s) 356, 427
HindIII AAGCTT 1 cut(s) 178
HinfI GANTC 2 cut(s) 280, 286
HpaII CCGG 1 cut(s) 524
HphI GGTGA 2 cut(s) 178, 424
Hpy166II GTNNAC 1 cut(s) 324
Hpy188I TCNGA 3 cut(s) 25, 133, 285
Hpy188III TCNNGA 1 cut(s) 534
Hpy8I GTNNAC 1 cut(s) 324
Hpy99I CGWCG 1 cut(s) 331
HpyAV CCTTC 4 cut(s) 146, 163, 254, 423
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4IV ACGT 1 cut(s) 326
HpyCH4V TGCA 4 cut(s) 218, 223, 356, 362
HpyF10VI GCNNNNNNNGC 2 cut(s) 418, 454
HpyF3I CTNAG 1 cut(s) 31
HpySE526I ACGT 1 cut(s) 326
Hsp92II CATG 2 cut(s) 356, 427
Ksp22I TGATCA 1 cut(s) 315
Kzo9I GATC 2 cut(s) 315, 526
LpnPI CCDG 6 cut(s) 18, 126, 471, 481, 508, 537
Lsp1109I GCAGC 2 cut(s) 76, 235
LweI GCATC 1 cut(s) 337
MaeI CTAG 1 cut(s) 293
MaeII ACGT 1 cut(s) 326
MalI GATC 2 cut(s) 317, 528
MboI GATC 2 cut(s) 315, 526
MboII GAAGA 2 cut(s) 281, 287
MluCI AATT 4 cut(s) 102, 134, 306, 537
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 7 cut(s) 31, 61, 187, 373, 397, 438, 459
MroXI GAANNNNTTC 1 cut(s) 279
MseI TTAA 3 cut(s) 108, 212, 231
MslI CAYNNNNRTG 1 cut(s) 473
MspI CCGG 1 cut(s) 524
MspR9I CCNGG 1 cut(s) 496
MvaI CCWGG 1 cut(s) 496
MvnI CGCG 1 cut(s) 331
MwoI GCNNNNNNNGC 2 cut(s) 418, 454
NdeII GATC 2 cut(s) 315, 526
NlaIII CATG 2 cut(s) 356, 427
PceI AGGCCT 1 cut(s) 448
PdmI GAANNNNTTC 1 cut(s) 279
PfeI GAWTC 2 cut(s) 280, 286
PkrI GCNGC 4 cut(s) 19, 91, 225, 256
PshBI ATTAAT 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 494
PspGI CCWGG 1 cut(s) 494
RseI CAYNNNNRTG 1 cut(s) 473
SaqAI TTAA 3 cut(s) 108, 212, 231
SatI GCNGC 4 cut(s) 18, 90, 224, 255
Sau3AI GATC 2 cut(s) 315, 526
ScrFI CCNGG 1 cut(s) 496
SfaNI GCATC 1 cut(s) 337
SmiMI CAYNNNNRTG 1 cut(s) 473
Sse9I AATT 4 cut(s) 102, 134, 306, 537
SseBI AGGCCT 1 cut(s) 448
SsiI CCGC 5 cut(s) 17, 92, 254, 257, 331
SspMI CTAG 1 cut(s) 293
StuI AGGCCT 1 cut(s) 448
StyD4I CCNGG 1 cut(s) 494
TaaI ACNGT 1 cut(s) 166
TaiI ACGT 1 cut(s) 329
TasI AATT 4 cut(s) 102, 134, 306, 537
TauI GCSGC 2 cut(s) 20, 257
TfiI GAWTC 2 cut(s) 280, 286
Tru1I TTAA 3 cut(s) 108, 212, 231
Tru9I TTAA 3 cut(s) 108, 212, 231
TscAI CASTG 2 cut(s) 225, 318
TseI GCWGC 2 cut(s) 89, 223
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 2 cut(s) 225, 318
VspI ATTAAT 1 cut(s) 231
XapI RAATTY 1 cut(s) 306
XmiI GTMKAC 1 cut(s) 323
XmnI GAANNNNTTC 1 cut(s) 279
XspI CTAG 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.