Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004671

Physical Location & Seq

Genomic Coordinates Forward (+)

865189 .. 877109

Gene Structure 11921 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1007700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2229 bp

ATGGATGATATGAACGTATGTCCAACTGAGGAGGCCATTCAGGCTTTTTTGGAATACTTGGTTGACCCAGTACTTTCACCTAAATCTTCTATGCGCAATACACCATCGTTATCTCAACAAGAATCAGTTGCAAAACAGGTTCATGCCGTTGTGTTACTATACAACTACTACCACAGAAAGCAATGTCCAAAGCTAGAAGATTTGAATTTTGATTCCTTTTGCAAGTTGGTTTTGTTTCTGAAACCAGCCTTGATGGTTCATATGAAATTCATGCAAAGCCCCAACGACACTAAATTGGATGACCTGGAGAAACAACTTTCAGTGACGGAAAAAGCAATTGTGGATGCATGCAGTATATCTGTAAGTTTAGATGCATTAAAGGATGTTCCAAACACAGAGGGATGGCCAATTTCCAAAGTTACAGTTCTTTTAGTTGACTCCAAGAAGGAGAACTGCTTTTTGCTATTCAATTCCATTACCCAGGGGGTTTGGTCAGTGATTGAAAAAGATGTAGATGTCTCAGTCCACAGTCCGGAAGATAAAGCGGTGGAAAAACATGTACACAAAAACAAAAGAGTTATTGGAAAACCTTCAAGAAGAGAATCAACAACTGATGAACCCACTCTCCGAAAACTTGCATATTCAGCAATTAGTGAAGCTACAGGTATTAGTCAAACTGATCTGCTGGTTTTGGAGCGCCAAGTTGTATTTTCTGTGAGTAGCGAAAAGGCGGCTGCTTGCTTCTTTATAATTCAGTGCACCAAAACTGTCAGTGAGGGTATTATTCCAGTTCCTATTAAAGATGTTATCGACAGTCTGCAGGGTCCTATGGTCCGAAAGAGTTTCAGCAGCTGGGCAGTTACGCCAGTCGTTGAGTACTACCATGTGCTTCCTTATGCTGGGACTTTGTTAGACTGGTATCCCAGGAGAGAATTCTCAAATGGTTTGCAAGATTCAAGCCTAGGGGATGGAAATATGATTTTAAACAGCCGTGAAAGGATTGGAAGACCTAGCAAGCAAGAACTTCATAAAAGCCAAGACAAATCTCCTGTAAATGATGTCTTCATAGAGAATCTTGAAGACAACACAAGTGTTTCTAAATCTAATCCCCAATCATTGAAGGAAAACGATACAACTGGATGTTCCAAGACGAGTTTGGTTGAAGCCTTCAATGGGCCTCAGAAAATGGAGGCGGGTGACAGTTTCATGGTCCCACCGCAGAATGATCAAAGCTGCAAGAAGATTTCAAGCACCACTCACGTTATCGATCACCATGTGGAGAATCTTGAAAATAAAACAATTAGATATAAACCTCAACTAAGGAAGCAGAAAGATGCTGCTGGATGCTGCAAGACAAGTTTGGCTAATGCTCTCAATGAACCTCAGAAAGTTGTGATGGATGACCCTTCCATGGTCCCCTCACAGAATGGACAACACTGTAAGAACCCTTCAAGCACCATTCAAGTTGTCAACCGCCAAGGAAAGAGGACATCCTCTACCAAAAGTGAATTACATGGCTCAGCAAATAATGCTAAGGTTGAGGTGGTGAACTCGAAAACAGGGCCATTCATTCCTGACTGCGTTGAAAACCTTGTTTCTGGAAAGGAGATCCGTACCATTAACTCGTTTGATCAAAATGGTATAAGTGGTCATGCCCTTGTCACCTGTCAATCTGGTTCTCAAGATCTTCACAAACTACAGGTTGCTATAGCTTCAAAAGGAAACATCTTGTCACAAACTGCATTGGGAGTTCTTATGAAAAGGAGGGATGATCTGTCTCTTCAGCAGCGCAATATAGAGGATGAGATTGCTCAGTGTGATAAAAAAATCCAGAGAATTTTATATGGTGGGGAAGATGACTTGGCTCTAAAGGTAGACTCCATAATAGAAGCTTGTAATGATGTATACGCAAGAGGTGAAAGGAACTGTAGACTGCGTGAAGATCAATGCTCTCCTCAAAATGTGAAGAGGAAGAGGTTGTCAGAGGCTATTCTCAGCGTGAAAAGTCCATGCCAGGAATTGGATGGTGCATGTTATGACAACAATTGGATATTACCAACTTATCGTGTATCTCCATCAGACGGTGGATTCCAAGCTGACGTTACAGTAAAAGGGATGGAATTCGAGTGTTCAAGTGTGGGTGGCCTGTGTTCGAATCCTCGTGAGGCAAGAGAATCGGCTGCAGCACAAGTATTGGCAAAATTGCGAAGTATGGCAGGGCAGGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DND1_DSRM

Protein Analysis

743

Amino Acids

82.12

Weight (kDa)

6.36

Isoelectric Point (pI)

50.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF7913	PF25500	6 - 124	7.5e-53	Domain of unknown function (DUF7913)
DUF7915	PF25502	160 - 309	1.2e-61	Domain of unknown function (DUF7915)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004671)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05950 AT1G05950 AT1G05950 AT1G05950 AT1G05950
fragaria_vesca	FvH4_4g10700 FvH4_4g10700 FvH4_4g10700 FvH4_4g10700 FvH4_4g10700 FvH4_4g10700 FvH4_4g10700 FvH4_4g10700 FvH4_4g10700 FvH4_4g10700
malus_domestica	MD04G1007700.v1.1
prunus_persica	Prupe.1G191700_v2.0.a1 Prupe.1G191700_v2.0.a1 Prupe.1G191700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0407011
rosa_laevigata	RLG00000008744
rosa_multiflora	Rmu_sc0000600.1_g000013
rosa_roxburghii	Rroxscaffold_5G00351720
rosa_rugosa	Rorug04G0076500 Rorug04G0076600 Rorug04G0076700
rosa_samantha	Rh4AG142000 Rh4BG139200 Rh4CG148600 Rh4DG136200
rosa_wichuraiana	Rw4G011570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	749
Acc16I	TGCGCA	1 cut(s)	95
AccB7I	CCANNNNNTGG	1 cut(s)	2020
AccI	GTMKAC	3 cut(s)	1875, 1905, 1930
AccIII	TCCGGA	1 cut(s)	532
AciI	CCGC	5 cut(s)	545, 731, 1193, 1217, 1474
AclWI	GGATC	1 cut(s)	1603
AcoI	YGGCCR	1 cut(s)	404
AcsI	RAATTY	5 cut(s)	205, 266, 932, 1836, 2120
AcuI	CTGAAG	1 cut(s)	1766
AdeI	CACNNNGTG	1 cut(s)	1276
AfaI	GTAC	4 cut(s)	72, 561, 878, 1615
AfiI	CCNNNNNNNGG	6 cut(s)	532, 899, 1173, 1411, 2020, 2081
AflIII	ACRYGT	1 cut(s)	556
AjnI	CCWGG	4 cut(s)	303, 480, 923, 2013
AloI	GAACNNNNNNTCC	2 cut(s)	609, 641
AluBI	AGCT	7 cut(s)	193, 659, 852, 1233, 1712, 1892, 2096
AluI	AGCT	7 cut(s)	193, 659, 852, 1233, 1712, 1892, 2096
Alw21I	GWGCWC	1 cut(s)	761
Alw26I	GTCTC	2 cut(s)	523, 1782
Alw44I	GTGCAC	1 cut(s)	757
AlwI	GGATC	1 cut(s)	1603
AlwNI	CAGNNNCTG	1 cut(s)	852
Aor13HI	TCCGGA	1 cut(s)	532
AoxI	GGCC	5 cut(s)	33, 404, 1175, 1562, 2143
ApaLI	GTGCAC	1 cut(s)	757
ApeKI	GCWGC	8 cut(s)	734, 849, 1233, 1337, 1347, 1786, 2180, 2183
ApoI	RAATTY	5 cut(s)	205, 266, 932, 1836, 2120
Asp700I	GAANNNNTTC	1 cut(s)	589
AspA2I	CCTAGG	1 cut(s)	961
AspLEI	GCGC	3 cut(s)	96, 699, 1791
AspS9I	GGNCC	6 cut(s)	824, 832, 1175, 1210, 1414, 1562
AsuHPI	GGTGA	6 cut(s)	69, 1208, 1262, 1558, 1654, 1928
AsuII	TTCGAA	1 cut(s)	2153
AvaII	GGWCC	4 cut(s)	824, 832, 1210, 1414
AvrII	CCTAGG	1 cut(s)	961
BaeGI	GKGCMC	1 cut(s)	761
BalI	TGGCCA	1 cut(s)	406
BarI	GAAGNNNNNNTAC	2 cut(s)	1432, 1464
BauI	CACGAG	1 cut(s)	2160
BbsI	GAAGAC	3 cut(s)	1012, 1054, 1086
Bbv12I	GWGCWC	1 cut(s)	761
BbvI	GCAGC	8 cut(s)	721, 861, 1220, 1324, 1334, 1798, 2167, 2195
BccI	CCATC	8 cut(s)	112, 247, 396, 962, 1390, 2018, 2083, 2110
BceAI	ACGGC	2 cut(s)	131, 975
BcgI	CGANNNNNNTGC	2 cut(s)	2157, 2191
BciT130I	CCWGG	4 cut(s)	305, 482, 925, 2015
BciVI	GTATCC	1 cut(s)	930
BclI	TGATCA	2 cut(s)	1225, 1630
BcoDI	GTCTC	2 cut(s)	523, 1782
BfaI	CTAG	3 cut(s)	194, 962, 1011
BfmI	CTRYAG	6 cut(s)	660, 818, 1697, 1707, 1927, 2181
BfoI	RGCGCY	1 cut(s)	700
BfuI	GTATCC	1 cut(s)	930
BglI	GCCNNNNNGGC	1 cut(s)	41
BglII	AGATCT	1 cut(s)	1684
BisI	GCNGC	9 cut(s)	732, 735, 850, 1234, 1338, 1348, 1787, 2181, 2184
BlnI	CCTAGG	1 cut(s)	961
BlpI	GCTNAGC	1 cut(s)	1519
BlsI	GCNGC	9 cut(s)	733, 736, 851, 1235, 1339, 1349, 1788, 2182, 2185
BmcAI	AGTACT	2 cut(s)	72, 878
Bme1390I	CCNGG	4 cut(s)	305, 482, 925, 2015
Bme18I	GGWCC	4 cut(s)	824, 832, 1210, 1414
BmgT120I	GGNCC	6 cut(s)	824, 832, 1175, 1210, 1414, 1562
BmiI	GGNNCC	3 cut(s)	825, 1212, 1416
BmrFI	CCNGG	4 cut(s)	305, 482, 925, 2015
BmrI	ACTGGG	1 cut(s)	62
BmsI	GCATC	4 cut(s)	334, 361, 1324, 1334
BmuI	ACTGGG	1 cut(s)	62
BpiI	GAAGAC	3 cut(s)	1012, 1054, 1086
BpmI	CTGGAG	1 cut(s)	326
Bpu10I	CCTNAGC	1 cut(s)	1533
Bpu1102I	GCTNAGC	1 cut(s)	1519
Bpu14I	TTCGAA	1 cut(s)	2153
BpuEI	CTTGAG	1 cut(s)	1665
Bsa29I	ATCGAT	1 cut(s)	1266
BsaJI	CCNNGG	6 cut(s)	480, 481, 923, 961, 1410, 1477
BsaWI	WCCGGW	1 cut(s)	532
BsaXI	ACNNNNNCTCC	2 cut(s)	609, 639
Bsc4I	CCNNNNNNNGG	6 cut(s)	532, 899, 1173, 1411, 2020, 2081
Bse1I	ACTGG	5 cut(s)	68, 788, 866, 920, 1141
Bse3DI	GCAATG	1 cut(s)	188
BseAI	TCCGGA	1 cut(s)	532
BseBI	CCWGG	4 cut(s)	305, 482, 925, 2015
BseCI	ATCGAT	1 cut(s)	1266
BseDI	CCNNGG	6 cut(s)	480, 481, 923, 961, 1410, 1477
BseLI	CCNNNNNNNGG	6 cut(s)	532, 899, 1173, 1411, 2020, 2081
BseMI	GCAATG	1 cut(s)	188
BseMII	CTCAG	7 cut(s)	18, 534, 1193, 1397, 1533, 1826, 2008
BseNI	ACTGG	5 cut(s)	68, 788, 866, 920, 1141
BseRI	GAGGAG	2 cut(s)	44, 1944
BseSI	GKGCMC	1 cut(s)	761
BseXI	GCAGC	8 cut(s)	721, 861, 1220, 1324, 1334, 1798, 2167, 2195
BseYI	CCCAGC	2 cut(s)	852, 899
BshFI	GGCC	5 cut(s)	35, 406, 1177, 1564, 2145
BshVI	ATCGAT	1 cut(s)	1266
BsiHKAI	GWGCWC	1 cut(s)	761
BsiSI	CCGG	1 cut(s)	533
BslFI	GGGAC	3 cut(s)	916, 1196, 1400
BslI	CCNNNNNNNGG	6 cut(s)	532, 899, 1173, 1411, 2020, 2081
BsmAI	GTCTC	2 cut(s)	523, 1782
BsmFI	GGGAC	3 cut(s)	916, 1196, 1400
BsnI	GGCC	5 cut(s)	35, 406, 1177, 1564, 2145
Bsp119I	TTCGAA	1 cut(s)	2153
Bsp1286I	GDGCHC	1 cut(s)	761
Bsp13I	TCCGGA	1 cut(s)	532
Bsp1407I	TGTACA	1 cut(s)	559
Bsp143I	GATC	8 cut(s)	679, 1225, 1267, 1608, 1630, 1684, 1771, 1942
Bsp1720I	GCTNAGC	1 cut(s)	1519
Bsp19I	CCATGG	1 cut(s)	1410
BspACI	CCGC	5 cut(s)	545, 731, 1193, 1217, 1474
BspANI	GGCC	5 cut(s)	35, 406, 1177, 1564, 2145
BspCNI	CTCAG	7 cut(s)	19, 533, 1192, 1396, 1532, 1825, 2007
BspDI	ATCGAT	1 cut(s)	1266
BspEI	TCCGGA	1 cut(s)	532
BspLI	GGNNCC	3 cut(s)	825, 1212, 1416
BspMAI	CTGCAG	2 cut(s)	822, 2185
BspPI	GGATC	1 cut(s)	1603
BspT104I	TTCGAA	1 cut(s)	2153
BsrDI	GCAATG	1 cut(s)	188
BsrGI	TGTACA	1 cut(s)	559
BsrI	ACTGG	5 cut(s)	68, 788, 866, 920, 1141
BssECI	CCNNGG	6 cut(s)	480, 481, 923, 961, 1410, 1477
BssMI	GATC	8 cut(s)	679, 1225, 1267, 1608, 1630, 1684, 1771, 1942
BssNAI	GTATAC	1 cut(s)	1906
BssSI	CACGAG	1 cut(s)	2160
BssT1I	CCWWGG	3 cut(s)	961, 1410, 1477
Bst1107I	GTATAC	1 cut(s)	1906
Bst2BI	CACGAG	1 cut(s)	2160
Bst2UI	CCWGG	4 cut(s)	305, 482, 925, 2015
Bst4CI	ACNGT	9 cut(s)	424, 530, 769, 815, 1202, 1439, 1928, 2084, 2107
Bst6I	CTCTTC	4 cut(s)	592, 1785, 1961, 1967
BstAPI	GCANNNNNTGC	1 cut(s)	1529
BstAUI	TGTACA	1 cut(s)	559
BstBI	TTCGAA	1 cut(s)	2153
BstC8I	GCNNGC	4 cut(s)	349, 739, 1016, 2223
BstDEI	CTNAG	9 cut(s)	27, 520, 1179, 1319, 1383, 1519, 1533, 1812, 1994
BstDSI	CCRYGG	1 cut(s)	1410
BstH2I	RGCGCY	1 cut(s)	700
BstHHI	GCGC	3 cut(s)	96, 699, 1791
BstKTI	GATC	8 cut(s)	682, 1228, 1270, 1611, 1633, 1687, 1774, 1945
BstMAI	GTCTC	2 cut(s)	523, 1782
BstMBI	GATC	8 cut(s)	679, 1225, 1267, 1608, 1630, 1684, 1771, 1942
BstMWI	GCNNNNNNNGC	4 cut(s)	41, 644, 1529, 2222
BstNI	CCWGG	4 cut(s)	305, 482, 925, 2015
BstNSI	RCATGY	3 cut(s)	351, 560, 2034
BstSCI	CCNGG	4 cut(s)	303, 480, 923, 2013
BstSFI	CTRYAG	6 cut(s)	660, 818, 1697, 1707, 1927, 2181
BstSLI	GKGCMC	1 cut(s)	761
BstV1I	GCAGC	8 cut(s)	721, 861, 1220, 1324, 1334, 1798, 2167, 2195
BstV2I	GAAGAC	3 cut(s)	1012, 1054, 1086
BstX2I	RGATCY	2 cut(s)	1608, 1684
BstYI	RGATCY	2 cut(s)	1608, 1684
BstZ17I	GTATAC	1 cut(s)	1906
Bsu15I	ATCGAT	1 cut(s)	1266
BsuI	GTATCC	1 cut(s)	930
BsuRI	GGCC	5 cut(s)	35, 406, 1177, 1564, 2145
BsuTUI	ATCGAT	1 cut(s)	1266
BtgI	CCRYGG	1 cut(s)	1410
BtsIMutI	CAGTG	6 cut(s)	327, 501, 761, 778, 1435, 1820
Cac8I	GCNNGC	4 cut(s)	349, 739, 1016, 2223
CaiI	CAGNNNCTG	1 cut(s)	852
CfoI	GCGC	3 cut(s)	96, 699, 1791
Cfr13I	GGNCC	6 cut(s)	824, 832, 1175, 1210, 1414, 1562
ClaI	ATCGAT	1 cut(s)	1266
Csp6I	GTAC	4 cut(s)	71, 560, 877, 1614
CviQI	GTAC	4 cut(s)	71, 560, 877, 1614
DdeI	CTNAG	9 cut(s)	27, 520, 1179, 1319, 1383, 1519, 1533, 1812, 1994
DpnI	GATC	8 cut(s)	681, 1227, 1269, 1610, 1632, 1686, 1773, 1944
DpnII	GATC	8 cut(s)	679, 1225, 1267, 1608, 1630, 1684, 1771, 1942
DraI	TTTAAA	1 cut(s)	984
DraIII	CACNNNGTG	1 cut(s)	1276
EaeI	YGGCCR	1 cut(s)	404
Eam1104I	CTCTTC	4 cut(s)	592, 1785, 1961, 1967
EarI	CTCTTC	4 cut(s)	592, 1785, 1961, 1967
Eco130I	CCWWGG	3 cut(s)	961, 1410, 1477
Eco47I	GGWCC	4 cut(s)	824, 832, 1210, 1414
Eco57I	CTGAAG	1 cut(s)	1766
EcoO109I	RGGNCCY	1 cut(s)	824
EcoRI	GAATTC	2 cut(s)	932, 2120
EcoRII	CCWGG	4 cut(s)	303, 480, 923, 2013
EcoT14I	CCWWGG	3 cut(s)	961, 1410, 1477
EcoT22I	ATGCAT	2 cut(s)	349, 376
ErhI	CCWWGG	3 cut(s)	961, 1410, 1477
FaqI	GGGAC	3 cut(s)	916, 1196, 1400
FauI	CCCGC	1 cut(s)	1186
FauNDI	CATATG	1 cut(s)	261
FbaI	TGATCA	2 cut(s)	1225, 1630
FblI	GTMKAC	3 cut(s)	1875, 1905, 1930
Fnu4HI	GCNGC	9 cut(s)	732, 735, 850, 1234, 1338, 1348, 1787, 2181, 2184
Fsp4HI	GCNGC	9 cut(s)	732, 735, 850, 1234, 1338, 1348, 1787, 2181, 2184
FspBI	CTAG	3 cut(s)	194, 962, 1011
FspI	TGCGCA	1 cut(s)	95
GlaI	GCGC	3 cut(s)	95, 698, 1790
GluI	GCNGC	9 cut(s)	732, 735, 850, 1234, 1338, 1348, 1787, 2181, 2184
GsaI	CCCAGC	2 cut(s)	856, 903
GsuI	CTGGAG	1 cut(s)	326
HaeII	RGCGCY	1 cut(s)	700
HaeIII	GGCC	5 cut(s)	35, 406, 1177, 1564, 2145
HapII	CCGG	1 cut(s)	533
HhaI	GCGC	3 cut(s)	96, 699, 1791
Hin6I	GCGC	3 cut(s)	94, 697, 1789
HinP1I	GCGC	3 cut(s)	94, 697, 1789
HincII	GTYRAC	3 cut(s)	64, 436, 1471
HindII	GTYRAC	3 cut(s)	64, 436, 1471
HindIII	AAGCTT	1 cut(s)	1890
HpaII	CCGG	1 cut(s)	533
HphI	GGTGA	6 cut(s)	69, 1208, 1262, 1558, 1654, 1928
Hpy188I	TCNGA	7 cut(s)	240, 629, 836, 1182, 1386, 1984, 2080
Hpy188III	TCNNGA	9 cut(s)	533, 594, 1076, 1286, 1574, 1599, 1682, 1831, 2162
HpyAV	CCTTC	6 cut(s)	439, 600, 1114, 1177, 1416, 1458
HpyCH4III	ACNGT	9 cut(s)	424, 530, 769, 815, 1202, 1439, 1928, 2084, 2107
HpyCH4IV	ACGT	3 cut(s)	15, 1260, 2100
HpyF10VI	GCNNNNNNNGC	4 cut(s)	41, 644, 1529, 2222
HpyF3I	CTNAG	9 cut(s)	27, 520, 1179, 1319, 1383, 1519, 1533, 1812, 1994
HpySE526I	ACGT	3 cut(s)	15, 1260, 2100
HspAI	GCGC	3 cut(s)	94, 697, 1789
Kpn2I	TCCGGA	1 cut(s)	532
Ksp22I	TGATCA	2 cut(s)	1225, 1630
Kzo9I	GATC	8 cut(s)	679, 1225, 1267, 1608, 1630, 1684, 1771, 1942
LmnI	GCTCC	1 cut(s)	694
Lsp1109I	GCAGC	8 cut(s)	721, 861, 1220, 1324, 1334, 1798, 2167, 2195
LweI	GCATC	4 cut(s)	334, 361, 1324, 1334
MaeI	CTAG	3 cut(s)	194, 962, 1011
MaeII	ACGT	3 cut(s)	15, 1260, 2100
MaeIII	GTNAC	8 cut(s)	153, 322, 418, 859, 1196, 1660, 1731, 2101
MalI	GATC	8 cut(s)	681, 1227, 1269, 1610, 1632, 1686, 1773, 1944
MboI	GATC	8 cut(s)	679, 1225, 1267, 1608, 1630, 1684, 1771, 1942
MfeI	CAATTG	2 cut(s)	336, 2044
MflI	RGATCY	2 cut(s)	1608, 1684
MhlI	GDGCHC	1 cut(s)	761
MlsI	TGGCCA	1 cut(s)	406
MluNI	TGGCCA	1 cut(s)	406
MlyI	GAGTC	2 cut(s)	431, 1871
MmeI	TCCRAC	1 cut(s)	47
Mox20I	TGGCCA	1 cut(s)	406
Mph1103I	ATGCAT	2 cut(s)	349, 376
MroI	TCCGGA	1 cut(s)	532
MroXI	GAANNNNTTC	1 cut(s)	589
MscI	TGGCCA	1 cut(s)	406
MseI	TTAA	4 cut(s)	377, 798, 983, 1620
Msp20I	TGGCCA	1 cut(s)	406
MspA1I	CMGCKG	1 cut(s)	852
MspI	CCGG	1 cut(s)	533
MspR9I	CCNGG	4 cut(s)	305, 482, 925, 2015
MunI	CAATTG	2 cut(s)	336, 2044
MvaI	CCWGG	4 cut(s)	305, 482, 925, 2015
MwoI	GCNNNNNNNGC	4 cut(s)	41, 644, 1529, 2222
NcoI	CCATGG	1 cut(s)	1410
NdeI	CATATG	1 cut(s)	261
NdeII	GATC	8 cut(s)	679, 1225, 1267, 1608, 1630, 1684, 1771, 1942
NlaIV	GGNNCC	3 cut(s)	825, 1212, 1416
NmuCI	GTSAC	4 cut(s)	322, 1196, 1660, 1731
NsbI	TGCGCA	1 cut(s)	95
NsiI	ATGCAT	2 cut(s)	349, 376
NspI	RCATGY	3 cut(s)	351, 560, 2034
NspV	TTCGAA	1 cut(s)	2153
PaeI	GCATGC	1 cut(s)	351
PasI	CCCWGGG	1 cut(s)	481
PciI	ACATGT	1 cut(s)	556
PdmI	GAANNNNTTC	1 cut(s)	589
PfeI	GAWTC	9 cut(s)	122, 212, 602, 953, 1072, 1282, 2088, 2155, 2174
PflMI	CCANNNNNTGG	1 cut(s)	2020
PkrI	GCNGC	9 cut(s)	733, 736, 851, 1235, 1339, 1349, 1788, 2182, 2185
PleI	GAGTC	2 cut(s)	431, 1871
PpsI	GAGTC	2 cut(s)	431, 1871
PpuMI	RGGWCCY	1 cut(s)	824
PscI	ACATGT	1 cut(s)	556
PsiI	TTATAA	1 cut(s)	749
Psp5II	RGGWCCY	1 cut(s)	824
Psp6I	CCWGG	4 cut(s)	303, 480, 923, 2013
PspFI	CCCAGC	2 cut(s)	852, 899
PspGI	CCWGG	4 cut(s)	303, 480, 923, 2013
PspN4I	GGNNCC	3 cut(s)	825, 1212, 1416
PspPI	GGNCC	6 cut(s)	824, 832, 1175, 1210, 1414, 1562
PspPPI	RGGWCCY	1 cut(s)	824
PstI	CTGCAG	2 cut(s)	822, 2185
PstNI	CAGNNNCTG	1 cut(s)	852
PsuI	RGATCY	2 cut(s)	1608, 1684
PvuII	CAGCTG	1 cut(s)	852
RsaI	GTAC	4 cut(s)	72, 561, 878, 1615
RsaNI	GTAC	4 cut(s)	71, 560, 877, 1614
SaqAI	TTAA	4 cut(s)	377, 798, 983, 1620
SatI	GCNGC	9 cut(s)	732, 735, 850, 1234, 1338, 1348, 1787, 2181, 2184
Sau3AI	GATC	8 cut(s)	679, 1225, 1267, 1608, 1630, 1684, 1771, 1942
Sau96I	GGNCC	6 cut(s)	824, 832, 1175, 1210, 1414, 1562
ScaI	AGTACT	2 cut(s)	72, 878
SchI	GAGTC	2 cut(s)	431, 1871
ScrFI	CCNGG	4 cut(s)	305, 482, 925, 2015
SduI	GDGCHC	1 cut(s)	761
SfaNI	GCATC	4 cut(s)	334, 361, 1324, 1334
SfcI	CTRYAG	6 cut(s)	660, 818, 1697, 1707, 1927, 2181
SfuI	TTCGAA	1 cut(s)	2153
SinI	GGWCC	4 cut(s)	824, 832, 1210, 1414
SmlI	CTYRAG	1 cut(s)	1680
SmoI	CTYRAG	1 cut(s)	1680
SphI	GCATGC	1 cut(s)	351
SsiI	CCGC	5 cut(s)	545, 731, 1193, 1217, 1474
SspMI	CTAG	3 cut(s)	194, 962, 1011
StyD4I	CCNGG	4 cut(s)	303, 480, 923, 2013
StyI	CCWWGG	3 cut(s)	961, 1410, 1477
TaaI	ACNGT	9 cut(s)	424, 530, 769, 815, 1202, 1439, 1928, 2084, 2107
TaiI	ACGT	3 cut(s)	18, 1263, 2103
TaqI	TCGA	5 cut(s)	810, 1266, 1553, 2124, 2153
TatI	WGTACW	3 cut(s)	70, 559, 876
TauI	GCSGC	1 cut(s)	734
TfiI	GAWTC	9 cut(s)	122, 212, 602, 953, 1072, 1282, 2088, 2155, 2174
Tru1I	TTAA	4 cut(s)	377, 798, 983, 1620
Tru9I	TTAA	4 cut(s)	377, 798, 983, 1620
TscAI	CASTG	6 cut(s)	327, 501, 761, 778, 1442, 1820
TseFI	GTSAC	4 cut(s)	322, 1196, 1660, 1731
TseI	GCWGC	8 cut(s)	734, 849, 1233, 1337, 1347, 1786, 2180, 2183
Tsp45I	GTSAC	4 cut(s)	322, 1196, 1660, 1731
TspGWI	ACGGA	2 cut(s)	341, 1601
TspRI	CASTG	6 cut(s)	327, 501, 761, 778, 1442, 1820
Van91I	CCANNNNNTGG	1 cut(s)	2020
VneI	GTGCAC	1 cut(s)	757
VpaK11BI	GGWCC	4 cut(s)	824, 832, 1210, 1414
XapI	RAATTY	5 cut(s)	205, 266, 932, 1836, 2120
XceI	RCATGY	3 cut(s)	351, 560, 2034
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1153, 2021
XmaJI	CCTAGG	1 cut(s)	961
XmiI	GTMKAC	3 cut(s)	1875, 1905, 1930
XmnI	GAANNNNTTC	1 cut(s)	589
XspI	CTAG	3 cut(s)	194, 962, 1011
ZrmI	AGTACT	2 cut(s)	72, 878
Zsp2I	ATGCAT	2 cut(s)	349, 376

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.