MD04G1046600.v1.1

Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2- thiolation reaction by being thiocarboxylated (-COSH) at its C- terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016072
Physical Location & Seq
Forward (+)
5430790 .. 5434572
3783 bp
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UTR
Exon/CDS
Intron
MD04G1046600.v1.1.491

Sequence Viewer

Length: 297 bp
ATGCAGCTGACCCTTGAATTCGGTGGAGGATTAGAGCTTCTATGCAACTCTGTAAAGATTCATAATGTCAATGTCGAATTGCAAAACGGAGCAGAAAAGCTAACCATGAAGGATTTGCTCTCTTGGATCCGTACTAATTTGATCAAGGAGAGGCCTGAAATGTTTATGAAAGGGGATACTGTACCCGGTGTTCTAGCCCTTGTGAACGATTGTGACTGGGAGCTGAGTGGGCAACTTGATACGACGCTAGAGGAGAAGGATGTCGTTGTCTTCATTTCAACCTTGCACGGTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
Urm1

Protein Analysis

99

Amino Acids

10.9

Weight (kDa)

4.61

Isoelectric Point (pI)

19.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Urm1 PF09138 3 - 98 1.8e-34 Urm1 (Ubiquitin related modifier)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016072)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45695 AT3G61113
fragaria_vesca FvH4_1g27190
malus_domestica MD04G1046600.v1.1
prunus_persica Prupe.1G200200_v2.0.a1 Prupe.1G200200_v2.0.a1 Prupe.1G200200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0487581
rosa_laevigata RLG00000022999
rosa_multiflora Rmu_sc0012412.1_g000002
rosa_roxburghii Rroxscaffold_6G00394690
rosa_rugosa Rorug03G0234100 Rorug03G0234200
rosa_samantha Rh3BG318600 Rh3DG314700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 121, 134
AcsI RAATTY 1 cut(s) 17
AfaI GTAC 2 cut(s) 133, 183
AgsI TTSAA 2 cut(s) 17, 279
AluBI AGCT 4 cut(s) 7, 37, 100, 223
AluI AGCT 4 cut(s) 7, 37, 100, 223
AlwI GGATC 2 cut(s) 121, 134
AoxI GGCC 1 cut(s) 152
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 1 cut(s) 17
AsuC2I CCSGG 1 cut(s) 186
BamHI GGATCC 1 cut(s) 126
BbsI GAAGAC 1 cut(s) 262
BbvI GCAGC 1 cut(s) 16
BciVI GTATCC 1 cut(s) 169
BclI TGATCA 1 cut(s) 141
BcnI CCSGG 1 cut(s) 186
BfaI CTAG 2 cut(s) 194, 248
BfuI GTATCC 1 cut(s) 169
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
Bme1390I CCNGG 1 cut(s) 186
BmiI GGNNCC 1 cut(s) 128
BmrFI CCNGG 1 cut(s) 186
BmrI ACTGGG 1 cut(s) 226
BmuI ACTGGG 1 cut(s) 226
BpiI GAAGAC 1 cut(s) 262
BpuMI CCSGG 1 cut(s) 186
Bse1I ACTGG 1 cut(s) 221
BseGI GGATG 1 cut(s) 265
BseMII CTCAG 1 cut(s) 215
BseNI ACTGG 1 cut(s) 221
BseRI GAGGAG 1 cut(s) 266
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 1 cut(s) 154
BsiSI CCGG 1 cut(s) 186
BsnI GGCC 1 cut(s) 154
Bsp143I GATC 2 cut(s) 126, 141
BspANI GGCC 1 cut(s) 154
BspCNI CTCAG 1 cut(s) 216
BspLI GGNNCC 1 cut(s) 128
BspPI GGATC 2 cut(s) 121, 134
BsrI ACTGG 1 cut(s) 221
BssMI GATC 2 cut(s) 126, 141
Bst4CI ACNGT 2 cut(s) 181, 290
BstDEI CTNAG 1 cut(s) 224
BstF5I GGATG 1 cut(s) 265
BstKTI GATC 2 cut(s) 129, 144
BstMBI GATC 2 cut(s) 126, 141
BstMWI GCNNNNNNNGC 1 cut(s) 229
BstSCI CCNGG 1 cut(s) 184
BstV1I GCAGC 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 262
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BsuI GTATCC 1 cut(s) 169
BsuRI GGCC 1 cut(s) 154
BtsCI GGATG 1 cut(s) 265
CseI GACGC 1 cut(s) 253
Csp6I GTAC 2 cut(s) 132, 182
CviAII CATG 1 cut(s) 106
CviJI RGCY 6 cut(s) 7, 37, 100, 154, 197, 223
CviKI_1 RGCY 6 cut(s) 7, 37, 100, 154, 197, 223
CviQI GTAC 2 cut(s) 132, 182
DdeI CTNAG 1 cut(s) 224
DpnI GATC 2 cut(s) 128, 143
DpnII GATC 2 cut(s) 126, 141
Eco147I AGGCCT 1 cut(s) 154
EcoRI GAATTC 1 cut(s) 17
FaeI CATG 1 cut(s) 109
FaiI YATR 4 cut(s) 43, 63, 107, 167
FatI CATG 1 cut(s) 105
FbaI TGATCA 1 cut(s) 141
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 1 cut(s) 272
Fsp4HI GCNGC 1 cut(s) 5
FspBI CTAG 2 cut(s) 194, 248
GluI GCNGC 1 cut(s) 5
HaeIII GGCC 1 cut(s) 154
HapII CCGG 1 cut(s) 186
HgaI GACGC 1 cut(s) 253
Hin1II CATG 1 cut(s) 109
HinfI GANTC 1 cut(s) 58
HpaII CCGG 1 cut(s) 186
Hpy166II GTNNAC 1 cut(s) 205
Hpy8I GTNNAC 1 cut(s) 205
Hpy99I CGWCG 1 cut(s) 247
HpyAV CCTTC 2 cut(s) 103, 250
HpyCH4III ACNGT 2 cut(s) 181, 290
HpyCH4V TGCA 4 cut(s) 4, 45, 82, 286
HpyF10VI GCNNNNNNNGC 1 cut(s) 229
HpyF3I CTNAG 1 cut(s) 224
Hsp92II CATG 1 cut(s) 109
Ksp22I TGATCA 1 cut(s) 141
Kzo9I GATC 2 cut(s) 126, 141
LmnI GCTCC 2 cut(s) 89, 220
LpnPI CCDG 3 cut(s) 168, 199, 202
Lsp1109I GCAGC 1 cut(s) 16
MaeI CTAG 2 cut(s) 194, 248
MaeIII GTNAC 1 cut(s) 212
MalI GATC 2 cut(s) 128, 143
MboI GATC 2 cut(s) 126, 141
MboII GAAGA 1 cut(s) 262
MflI RGATCY 1 cut(s) 126
MluCI AATT 3 cut(s) 17, 77, 136
MnlI CCTC 3 cut(s) 20, 144, 244
MspA1I CMGCKG 1 cut(s) 7
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 1 cut(s) 186
MwoI GCNNNNNNNGC 1 cut(s) 229
NciI CCSGG 1 cut(s) 186
NdeII GATC 2 cut(s) 126, 141
NlaIII CATG 1 cut(s) 109
NlaIV GGNNCC 1 cut(s) 128
NmuCI GTSAC 1 cut(s) 212
PceI AGGCCT 1 cut(s) 154
PfeI GAWTC 1 cut(s) 58
PkrI GCNGC 1 cut(s) 6
PspN4I GGNNCC 1 cut(s) 128
PsrI GAACNNNNNNTAC 2 cut(s) 174, 206
PsuI RGATCY 1 cut(s) 126
PvuII CAGCTG 1 cut(s) 7
RsaI GTAC 2 cut(s) 133, 183
RsaNI GTAC 2 cut(s) 132, 182
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 2 cut(s) 126, 141
ScrFI CCNGG 1 cut(s) 186
SetI ASST 5 cut(s) 9, 39, 102, 225, 284
Sse9I AATT 3 cut(s) 17, 77, 136
SseBI AGGCCT 1 cut(s) 154
SspMI CTAG 2 cut(s) 194, 248
StuI AGGCCT 1 cut(s) 154
StyD4I CCNGG 1 cut(s) 184
TaaI ACNGT 2 cut(s) 181, 290
TaqI TCGA 1 cut(s) 75
TasI AATT 3 cut(s) 17, 77, 136
TfiI GAWTC 1 cut(s) 58
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 4 cut(s) 50, 122, 182, 262
TspGWI ACGGA 2 cut(s) 102, 119
XapI RAATTY 1 cut(s) 17
XspI CTAG 2 cut(s) 194, 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.