Rmu_sc0012412.1_g000002

Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2- thiolation reaction by being thiocarboxylated (-COSH) at its C- terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0012412.1
OG0016072
Physical Location & Seq
Forward (+)
13633 .. 18501
4869 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0012412.1_g000002.1.cds

Sequence Viewer

Length: 468 bp
atgatggtggctcggttgagaggccggtgcacgttttcttcatgggctgaggagattggtgctgggccgggctgcacgtgggcctcgaaaaagacaaaagtagaatacaagagcgatttgcagcacagcaccagagaaagccccagaaacctctgcagtctttccagtatgcagctgaccatagaattcggtggaggattagagcttctttgtaactctgtcaagattcataatgtcaatgtagaactgcaaaaggcagcagaaaagttaaccatgaaagatttactggcttggattcgtactaatttgatcaaggagaggcctgaaatgtttatgaaaggagattctgtgagacctggtgttctagcccttgtgaatgattgtgattgggagctgactggtcaacttgatacaacactagaggagaaggatgtggttgttttcatttcaaccttgcacggtggatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
Urm1

Protein Analysis

155

Amino Acids

17.29

Weight (kDa)

6.31

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016072)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45695 AT3G61113
fragaria_vesca FvH4_1g27190
malus_domestica MD04G1046600.v1.1
prunus_persica Prupe.1G200200_v2.0.a1 Prupe.1G200200_v2.0.a1 Prupe.1G200200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0487581
rosa_laevigata RLG00000022999
rosa_multiflora Rmu_sc0012412.1_g000002
rosa_roxburghii Rroxscaffold_6G00394690
rosa_rugosa Rorug03G0234100 Rorug03G0234200
rosa_samantha Rh3BG318600 Rh3DG314700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 185
AcvI CACGTG 1 cut(s) 78
AfaI GTAC 1 cut(s) 301
AgsI TTSAA 1 cut(s) 450
AjnI CCWGG 1 cut(s) 355
AluBI AGCT 3 cut(s) 175, 205, 394
AluI AGCT 3 cut(s) 175, 205, 394
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 1 cut(s) 346
Alw44I GTGCAC 1 cut(s) 28
AoxI GGCC 4 cut(s) 22, 65, 81, 320
ApaLI GTGCAC 1 cut(s) 28
ApeKI GCWGC 4 cut(s) 72, 121, 172, 257
ApoI RAATTY 1 cut(s) 185
AspS9I GGNCC 2 cut(s) 65, 81
AsuC2I CCSGG 1 cut(s) 69
BaeGI GKGCMC 1 cut(s) 32
BbrPI CACGTG 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 32
BbvCI CCTCAGC 1 cut(s) 48
BbvI GCAGC 4 cut(s) 59, 133, 184, 269
BciT130I CCWGG 1 cut(s) 357
BclI TGATCA 1 cut(s) 309
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 346
BfaI CTAG 2 cut(s) 365, 419
BfmI CTRYAG 1 cut(s) 154
BisI GCNGC 4 cut(s) 73, 122, 173, 258
BlsI GCNGC 4 cut(s) 74, 123, 174, 259
Bme1390I CCNGG 2 cut(s) 69, 357
BmgT120I GGNCC 2 cut(s) 65, 81
BmrFI CCNGG 2 cut(s) 69, 357
Bpu10I CCTNAGC 1 cut(s) 48
BpuMI CCSGG 1 cut(s) 69
BsaAI YACGTR 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 346
BsaXI ACNNNNNCTCC 2 cut(s) 333, 363
Bse118I RCCGGY 1 cut(s) 24
Bse1I ACTGG 3 cut(s) 165, 291, 403
BseBI CCWGG 1 cut(s) 357
BseGI GGATG 1 cut(s) 436
BseMII CTCAG 1 cut(s) 39
BseNI ACTGG 3 cut(s) 165, 291, 403
BseRI GAGGAG 2 cut(s) 65, 437
BseSI GKGCMC 1 cut(s) 32
BseXI GCAGC 4 cut(s) 59, 133, 184, 269
BseYI CCCAGC 1 cut(s) 62
BsgI GTGCAG 1 cut(s) 58
BshFI GGCC 4 cut(s) 24, 67, 83, 322
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 2 cut(s) 25, 68
BsmAI GTCTC 1 cut(s) 346
BsnI GGCC 4 cut(s) 24, 67, 83, 322
Bso31I GGTCTC 1 cut(s) 346
Bsp1286I GDGCHC 1 cut(s) 32
Bsp143I GATC 1 cut(s) 309
BspANI GGCC 4 cut(s) 24, 67, 83, 322
BspCNI CTCAG 1 cut(s) 40
BspMAI CTGCAG 1 cut(s) 158
BspTNI GGTCTC 1 cut(s) 346
BsrFI RCCGGY 1 cut(s) 24
BsrI ACTGG 3 cut(s) 165, 291, 403
BssAI RCCGGY 1 cut(s) 24
BssMI GATC 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 1 cut(s) 461
BstBAI YACGTR 1 cut(s) 78
BstDEI CTNAG 1 cut(s) 48
BstF5I GGATG 1 cut(s) 436
BstKTI GATC 1 cut(s) 312
BstMAI GTCTC 1 cut(s) 346
BstMBI GATC 1 cut(s) 309
BstNI CCWGG 1 cut(s) 357
BstSCI CCNGG 2 cut(s) 67, 355
BstSFI CTRYAG 1 cut(s) 154
BstSLI GKGCMC 1 cut(s) 32
BstV1I GCAGC 4 cut(s) 59, 133, 184, 269
BsuRI GGCC 4 cut(s) 24, 67, 83, 322
BtsCI GGATG 1 cut(s) 436
Cfr10I RCCGGY 1 cut(s) 24
Cfr13I GGNCC 2 cut(s) 65, 81
CsiI ACCWGGT 1 cut(s) 355
Csp6I GTAC 1 cut(s) 300
CviAII CATG 2 cut(s) 42, 274
CviQI GTAC 1 cut(s) 300
DdeI CTNAG 1 cut(s) 48
DpnI GATC 1 cut(s) 311
DpnII GATC 1 cut(s) 309
Eco147I AGGCCT 1 cut(s) 322
Eco31I GGTCTC 1 cut(s) 346
Eco72I CACGTG 1 cut(s) 78
EcoRI GAATTC 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 355
FaeI CATG 2 cut(s) 45, 277
FaiI YATR 6 cut(s) 43, 170, 182, 231, 275, 335
FatI CATG 2 cut(s) 41, 273
FbaI TGATCA 1 cut(s) 309
Fnu4HI GCNGC 4 cut(s) 73, 122, 173, 258
FokI GGATG 1 cut(s) 443
Fsp4HI GCNGC 4 cut(s) 73, 122, 173, 258
FspBI CTAG 2 cut(s) 365, 419
GluI GCNGC 4 cut(s) 73, 122, 173, 258
GsaI CCCAGC 1 cut(s) 66
HaeIII GGCC 4 cut(s) 24, 67, 83, 322
HapII CCGG 2 cut(s) 25, 68
Hin1II CATG 2 cut(s) 45, 277
HincII GTYRAC 2 cut(s) 270, 404
HindII GTYRAC 2 cut(s) 270, 404
HinfI GANTC 3 cut(s) 226, 295, 344
HpaI GTTAAC 1 cut(s) 270
HpaII CCGG 2 cut(s) 25, 68
Hpy166II GTNNAC 3 cut(s) 30, 270, 404
Hpy188III TCNNGA 1 cut(s) 223
Hpy8I GTNNAC 3 cut(s) 30, 270, 404
HpyAV CCTTC 1 cut(s) 421
HpyCH4III ACNGT 1 cut(s) 461
HpyCH4IV ACGT 2 cut(s) 32, 77
HpyCH4V TGCA 7 cut(s) 30, 75, 121, 156, 172, 250, 457
HpyF3I CTNAG 1 cut(s) 48
HpySE526I ACGT 2 cut(s) 32, 77
Hsp92II CATG 2 cut(s) 45, 277
Ksp22I TGATCA 1 cut(s) 309
KspAI GTTAAC 1 cut(s) 270
Kzo9I GATC 1 cut(s) 309
LmnI GCTCC 1 cut(s) 391
Lsp1109I GCAGC 4 cut(s) 59, 133, 184, 269
MabI ACCWGGT 1 cut(s) 355
MaeI CTAG 2 cut(s) 365, 419
MaeII ACGT 2 cut(s) 32, 77
MaeIII GTNAC 1 cut(s) 212
MalI GATC 1 cut(s) 311
MboI GATC 1 cut(s) 309
MboII GAAGA 1 cut(s) 30
MhlI GDGCHC 1 cut(s) 32
MluCI AATT 2 cut(s) 185, 304
MnlI CCTC 7 cut(s) 14, 43, 94, 161, 188, 312, 415
MseI TTAA 1 cut(s) 269
MspA1I CMGCKG 1 cut(s) 175
MspI CCGG 2 cut(s) 25, 68
MspR9I CCNGG 2 cut(s) 69, 357
MvaI CCWGG 1 cut(s) 357
NciI CCSGG 1 cut(s) 69
NdeII GATC 1 cut(s) 309
NlaIII CATG 2 cut(s) 45, 277
PceI AGGCCT 1 cut(s) 322
PcsI WCGNNNNNNNCGW 1 cut(s) 83
PfeI GAWTC 3 cut(s) 226, 295, 344
PkrI GCNGC 4 cut(s) 74, 123, 174, 259
PmaCI CACGTG 1 cut(s) 78
PmlI CACGTG 1 cut(s) 78
Ppu21I YACGTR 1 cut(s) 78
Psp6I CCWGG 1 cut(s) 355
PspCI CACGTG 1 cut(s) 78
PspFI CCCAGC 1 cut(s) 62
PspGI CCWGG 1 cut(s) 355
PspPI GGNCC 2 cut(s) 65, 81
PstI CTGCAG 1 cut(s) 158
PvuII CAGCTG 1 cut(s) 175
RsaI GTAC 1 cut(s) 301
RsaNI GTAC 1 cut(s) 300
SaqAI TTAA 1 cut(s) 269
SatI GCNGC 4 cut(s) 73, 122, 173, 258
Sau3AI GATC 1 cut(s) 309
Sau96I GGNCC 2 cut(s) 65, 81
ScrFI CCNGG 2 cut(s) 69, 357
SduI GDGCHC 1 cut(s) 32
SetI ASST 8 cut(s) 35, 80, 153, 177, 207, 358, 396, 455
SexAI ACCWGGT 1 cut(s) 355
SfcI CTRYAG 1 cut(s) 154
Sse9I AATT 2 cut(s) 185, 304
SseBI AGGCCT 1 cut(s) 322
SspMI CTAG 2 cut(s) 365, 419
StuI AGGCCT 1 cut(s) 322
StyD4I CCNGG 2 cut(s) 67, 355
TaaI ACNGT 1 cut(s) 461
TaiI ACGT 2 cut(s) 35, 80
TaqI TCGA 1 cut(s) 86
TasI AATT 2 cut(s) 185, 304
TfiI GAWTC 3 cut(s) 226, 295, 344
Tru1I TTAA 1 cut(s) 269
Tru9I TTAA 1 cut(s) 269
TseI GCWGC 4 cut(s) 72, 121, 172, 257
TspDTI ATGAA 5 cut(s) 30, 218, 290, 350, 433
VneI GTGCAC 1 cut(s) 28
XapI RAATTY 1 cut(s) 185
XspI CTAG 2 cut(s) 365, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.