Rorug03G0234200

Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2- thiolation reaction by being thiocarboxylated (-COSH) at its C- terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0016072
Physical Location & Seq
Forward (+)
21670785 .. 21671252
468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0234200.1

Sequence Viewer

Length: 468 bp
ATGGGATGTACACGGTCCAGGAACATGGGGATCGCTTCTTATTCACTCTTACTGACCCATCGGACCGGAACTGTATTCTGCGAGGAGGACCTTGGAGCTTTGATCGAGCGCCAGTGGTTTTGGTCATATGATGGCATGGGACATATCAATGCAGTCCCTTTGAACATGTTAGCTTTCTGGATCAAGGTGAAGGATTTACCGCCAGCGTTTCATTCGGACAGGTGCCTTCGTCGACTCGGGTGTTCCCTAGGGAAGTTTATTCAAAAAGACACGGTGGAATATGATGCATGTCGTATCCGAATCCGGGTCGAGGTTGATTTGATGCAACCGGTGGTGTTACATAGGTGTTTCACCATGGAAGAGGGGCTAGATGTGGATCTTGAGTTTGTATTCGAAAACCTGTTTGGCCAGTGTGTGGAGTGTGGTCGCTTAACCATGTCGGCCTCTTATGTGCAAGTCCGCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
Urm1

Protein Analysis

155

Amino Acids

17.85

Weight (kDa)

6.07

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016072)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45695 AT3G61113
fragaria_vesca FvH4_1g27190
malus_domestica MD04G1046600.v1.1
prunus_persica Prupe.1G200200_v2.0.a1 Prupe.1G200200_v2.0.a1 Prupe.1G200200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0487581
rosa_laevigata RLG00000022999
rosa_multiflora Rmu_sc0012412.1_g000002
rosa_roxburghii Rroxscaffold_6G00394690
rosa_rugosa Rorug03G0234100 Rorug03G0234200
rosa_samantha Rh3BG318600 Rh3DG314700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 222
AccB7I CCANNNNNTGG 1 cut(s) 415
AccI GTMKAC 1 cut(s) 232
AciI CCGC 2 cut(s) 200, 460
AclWI GGATC 3 cut(s) 38, 188, 384
AcoI YGGCCR 1 cut(s) 406
AfaI GTAC 1 cut(s) 10
AfiI CCNNNNNNNGG 3 cut(s) 304, 310, 415
AflIII ACRYGT 1 cut(s) 165
AgeI ACCGGT 1 cut(s) 328
AgsI TTSAA 2 cut(s) 163, 263
AjnI CCWGG 1 cut(s) 17
AjuI GAANNNNNNNTTGG 2 cut(s) 387, 419
AluBI AGCT 2 cut(s) 98, 173
AluI AGCT 2 cut(s) 98, 173
AlwI GGATC 3 cut(s) 38, 188, 384
Ama87I CYCGRG 1 cut(s) 236
AoxI GGCC 2 cut(s) 406, 441
AsiGI ACCGGT 1 cut(s) 328
AspA2I CCTAGG 1 cut(s) 247
AspLEI GCGC 1 cut(s) 111
AspS9I GGNCC 3 cut(s) 15, 63, 88
AsuC2I CCSGG 1 cut(s) 305
AsuHPI GGTGA 2 cut(s) 199, 343
AsuII TTCGAA 1 cut(s) 393
AvaI CYCGRG 1 cut(s) 236
AvaII GGWCC 3 cut(s) 15, 63, 88
AvrII CCTAGG 1 cut(s) 247
BalI TGGCCA 1 cut(s) 408
BanI GGYRCC 1 cut(s) 222
BccI CCATC 2 cut(s) 66, 125
BciT130I CCWGG 1 cut(s) 19
BciVI GTATCC 1 cut(s) 305
BcnI CCSGG 1 cut(s) 305
BfaI CTAG 2 cut(s) 248, 368
BfoI RGCGCY 1 cut(s) 112
BfuI GTATCC 1 cut(s) 305
BlnI CCTAGG 1 cut(s) 247
Bme1390I CCNGG 2 cut(s) 19, 305
Bme18I GGWCC 3 cut(s) 15, 63, 88
BmeT110I CYCGRG 1 cut(s) 236
BmgT120I GGNCC 3 cut(s) 15, 63, 88
BmiI GGNNCC 1 cut(s) 224
BmrFI CCNGG 2 cut(s) 19, 305
BmsI GCATC 2 cut(s) 274, 312
Bpu14I TTCGAA 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 401
BpuMI CCSGG 1 cut(s) 305
BsaBI GATNNNNATC 1 cut(s) 375
BsaJI CCNNGG 3 cut(s) 91, 247, 354
BsaWI WCCGGW 2 cut(s) 65, 328
Bsc4I CCNNNNNNNGG 3 cut(s) 304, 310, 415
Bse118I RCCGGY 1 cut(s) 328
Bse1I ACTGG 2 cut(s) 112, 409
Bse8I GATNNNNATC 1 cut(s) 375
BseBI CCWGG 1 cut(s) 19
BseDI CCNNGG 3 cut(s) 91, 247, 354
BseGI GGATG 1 cut(s) 11
BseJI GATNNNNATC 1 cut(s) 375
BseLI CCNNNNNNNGG 3 cut(s) 304, 310, 415
BseNI ACTGG 2 cut(s) 112, 409
BseRI GAGGAG 1 cut(s) 98
BshFI GGCC 2 cut(s) 408, 443
BshNI GGYRCC 1 cut(s) 222
BshTI ACCGGT 1 cut(s) 328
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 3 cut(s) 66, 304, 329
BslFI GGGAC 2 cut(s) 140, 153
BslI CCNNNNNNNGG 3 cut(s) 304, 310, 415
BsmFI GGGAC 2 cut(s) 140, 153
BsnI GGCC 2 cut(s) 408, 443
BsoBI CYCGRG 1 cut(s) 236
Bsp119I TTCGAA 1 cut(s) 393
Bsp1407I TGTACA 1 cut(s) 8
Bsp143I GATC 4 cut(s) 30, 102, 180, 376
Bsp19I CCATGG 1 cut(s) 354
BspACI CCGC 2 cut(s) 200, 460
BspANI GGCC 2 cut(s) 408, 443
BspLI GGNNCC 1 cut(s) 224
BspPI GGATC 3 cut(s) 38, 188, 384
BspT104I TTCGAA 1 cut(s) 393
BspT107I GGYRCC 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 328
BsrGI TGTACA 1 cut(s) 8
BsrI ACTGG 2 cut(s) 112, 409
BssAI RCCGGY 1 cut(s) 328
BssECI CCNNGG 3 cut(s) 91, 247, 354
BssMI GATC 4 cut(s) 30, 102, 180, 376
BssT1I CCWWGG 3 cut(s) 91, 247, 354
Bst2UI CCWGG 1 cut(s) 19
Bst4CI ACNGT 3 cut(s) 15, 73, 274
Bst6I CTCTTC 1 cut(s) 354
BstAUI TGTACA 1 cut(s) 8
BstBI TTCGAA 1 cut(s) 393
BstC8I GCNNGC 1 cut(s) 204
BstDSI CCRYGG 1 cut(s) 354
BstF5I GGATG 1 cut(s) 11
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 1 cut(s) 111
BstKTI GATC 4 cut(s) 33, 105, 183, 379
BstMBI GATC 4 cut(s) 30, 102, 180, 376
BstNI CCWGG 1 cut(s) 19
BstNSI RCATGY 2 cut(s) 169, 291
BstSCI CCNGG 2 cut(s) 17, 303
BstX2I RGATCY 1 cut(s) 376
BstXI CCANNNNNNTGG 1 cut(s) 25
BstYI RGATCY 1 cut(s) 376
BsuI GTATCC 1 cut(s) 305
BsuRI GGCC 2 cut(s) 408, 443
BtgI CCRYGG 1 cut(s) 354
BtsCI GGATG 1 cut(s) 11
BtsIMutI CAGTG 2 cut(s) 119, 416
Cac8I GCNNGC 1 cut(s) 204
CfoI GCGC 1 cut(s) 111
Cfr10I RCCGGY 1 cut(s) 328
Cfr13I GGNCC 3 cut(s) 15, 63, 88
CpoI CGGWCCG 1 cut(s) 63
Csp6I GTAC 1 cut(s) 9
CspAI ACCGGT 1 cut(s) 328
CspI CGGWCCG 1 cut(s) 63
CviAII CATG 6 cut(s) 25, 136, 166, 288, 355, 436
CviJI RGCY 5 cut(s) 98, 173, 367, 408, 443
CviKI_1 RGCY 5 cut(s) 98, 173, 367, 408, 443
CviQI GTAC 1 cut(s) 9
DpnI GATC 4 cut(s) 32, 104, 182, 378
DpnII GATC 4 cut(s) 30, 102, 180, 376
EaeI YGGCCR 1 cut(s) 406
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
EciI GGCGGA 1 cut(s) 449
Eco130I CCWWGG 3 cut(s) 91, 247, 354
Eco47I GGWCC 3 cut(s) 15, 63, 88
Eco88I CYCGRG 1 cut(s) 236
EcoO109I RGGNCCY 1 cut(s) 88
EcoRII CCWGG 1 cut(s) 17
EcoT14I CCWWGG 3 cut(s) 91, 247, 354
EcoT22I ATGCAT 1 cut(s) 289
ErhI CCWWGG 3 cut(s) 91, 247, 354
FaeI CATG 6 cut(s) 28, 139, 169, 291, 358, 439
FalI AAGNNNNNCTT 1 cut(s) 447
FaqI GGGAC 2 cut(s) 140, 153
FatI CATG 6 cut(s) 24, 135, 165, 287, 354, 435
FauNDI CATATG 1 cut(s) 127
FblI GTMKAC 1 cut(s) 232
FokI GGATG 1 cut(s) 18
FspBI CTAG 2 cut(s) 248, 368
GlaI GCGC 1 cut(s) 110
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 2 cut(s) 408, 443
HapII CCGG 3 cut(s) 66, 304, 329
HhaI GCGC 1 cut(s) 111
Hin1II CATG 6 cut(s) 28, 139, 169, 291, 358, 439
Hin6I GCGC 1 cut(s) 109
HinP1I GCGC 1 cut(s) 109
HincII GTYRAC 1 cut(s) 233
HindII GTYRAC 1 cut(s) 233
HinfI GANTC 2 cut(s) 234, 300
HpaII CCGG 3 cut(s) 66, 304, 329
HphI GGTGA 2 cut(s) 199, 343
Hpy166II GTNNAC 2 cut(s) 11, 233
Hpy188I TCNGA 3 cut(s) 63, 217, 299
Hpy188III TCNNGA 2 cut(s) 178, 380
Hpy8I GTNNAC 2 cut(s) 11, 233
Hpy99I CGWCG 1 cut(s) 234
HpyAV CCTTC 2 cut(s) 184, 236
HpyCH4III ACNGT 3 cut(s) 15, 73, 274
HpyCH4V TGCA 4 cut(s) 152, 287, 325, 454
Hsp92II CATG 6 cut(s) 28, 139, 169, 291, 358, 439
HspAI GCGC 1 cut(s) 109
Kzo9I GATC 4 cut(s) 30, 102, 180, 376
LmnI GCTCC 1 cut(s) 95
LweI GCATC 2 cut(s) 274, 312
MaeI CTAG 2 cut(s) 248, 368
MaeIII GTNAC 1 cut(s) 336
MalI GATC 4 cut(s) 32, 104, 182, 378
MboI GATC 4 cut(s) 30, 102, 180, 376
MboII GAAGA 1 cut(s) 371
MflI RGATCY 1 cut(s) 376
MlsI TGGCCA 1 cut(s) 408
MluNI TGGCCA 1 cut(s) 408
MlyI GAGTC 1 cut(s) 228
MnlI CCTC 5 cut(s) 76, 79, 304, 355, 454
Mox20I TGGCCA 1 cut(s) 408
Mph1103I ATGCAT 1 cut(s) 289
MscI TGGCCA 1 cut(s) 408
MseI TTAA 1 cut(s) 431
MslI CAYNNNNRTG 1 cut(s) 147
Msp20I TGGCCA 1 cut(s) 408
MspI CCGG 3 cut(s) 66, 304, 329
MspR9I CCNGG 2 cut(s) 19, 305
MvaI CCWGG 1 cut(s) 19
NciI CCSGG 1 cut(s) 305
NcoI CCATGG 1 cut(s) 354
NdeI CATATG 1 cut(s) 127
NdeII GATC 4 cut(s) 30, 102, 180, 376
NlaIII CATG 6 cut(s) 28, 139, 169, 291, 358, 439
NlaIV GGNNCC 1 cut(s) 224
NsiI ATGCAT 1 cut(s) 289
NspI RCATGY 2 cut(s) 169, 291
NspV TTCGAA 1 cut(s) 393
PciI ACATGT 1 cut(s) 165
PfeI GAWTC 1 cut(s) 300
PflMI CCANNNNNTGG 1 cut(s) 415
PfoI TCCNGGA 1 cut(s) 17
PinAI ACCGGT 1 cut(s) 328
PleI GAGTC 1 cut(s) 228
PpsI GAGTC 1 cut(s) 228
PpuMI RGGWCCY 1 cut(s) 88
PscI ACATGT 1 cut(s) 165
Psp5II RGGWCCY 1 cut(s) 88
Psp6I CCWGG 1 cut(s) 17
PspGI CCWGG 1 cut(s) 17
PspN4I GGNNCC 1 cut(s) 224
PspPI GGNCC 3 cut(s) 15, 63, 88
PspPPI RGGWCCY 1 cut(s) 88
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 1 cut(s) 10
RsaNI GTAC 1 cut(s) 9
RseI CAYNNNNRTG 1 cut(s) 147
Rsr2I CGGWCCG 1 cut(s) 63
RsrII CGGWCCG 1 cut(s) 63
SalI GTCGAC 1 cut(s) 231
SaqAI TTAA 1 cut(s) 431
Sau3AI GATC 4 cut(s) 30, 102, 180, 376
Sau96I GGNCC 3 cut(s) 15, 63, 88
SchI GAGTC 1 cut(s) 228
ScrFI CCNGG 2 cut(s) 19, 305
SetI ASST 8 cut(s) 93, 100, 175, 189, 224, 315, 347, 402
SfaNI GCATC 2 cut(s) 274, 312
SfuI TTCGAA 1 cut(s) 393
SinI GGWCC 3 cut(s) 15, 63, 88
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
SsiI CCGC 2 cut(s) 200, 460
SspMI CTAG 2 cut(s) 248, 368
StyD4I CCNGG 2 cut(s) 17, 303
StyI CCWWGG 3 cut(s) 91, 247, 354
TaaI ACNGT 3 cut(s) 15, 73, 274
TaqI TCGA 4 cut(s) 105, 232, 309, 393
TatI WGTACW 1 cut(s) 8
TfiI GAWTC 1 cut(s) 300
Tru1I TTAA 1 cut(s) 431
Tru9I TTAA 1 cut(s) 431
TscAI CASTG 2 cut(s) 119, 416
TspDTI ATGAA 1 cut(s) 200
TspRI CASTG 2 cut(s) 119, 416
Van91I CCANNNNNTGG 1 cut(s) 415
VpaK11BI GGWCC 3 cut(s) 15, 63, 88
XceI RCATGY 2 cut(s) 169, 291
XmaJI CCTAGG 1 cut(s) 247
XmiI GTMKAC 1 cut(s) 232
XspI CTAG 2 cut(s) 248, 368
Zsp2I ATGCAT 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.