Rorug03G0234100

Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2- thiolation reaction by being thiocarboxylated (-COSH) at its C- terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000165
Physical Location & Seq
Forward (+)
21663768 .. 21669347
5580 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0234100.1

Sequence Viewer

Length: 795 bp
ATGTCTCACTTCGGAAGGTCTGGCCCTCCGGACATCACCGATACCTACTCTCTCCTCGTCCTCAACATCACCTTCCGGACGAGTGCGGATGATCTGTTTCCGCTTTTCGATAAGTACGGCAAGGTCGTCGACATCTTCATCCCCAGAGACCGAAGGACTGGCGAGTCCAGGGGATTTGCTTTTGTGCGATACAAATATGCAGATGAGGCACATAAAGCAGTTGAGAGGCTCGATGGAAGAGTTGTTGATGGTCGCGAAATAACTGTTCAGTTTGCAAAATATGGGCCAAATGCAGAGAGGATTCATAAAGGAAGGATTACTGAAACTCTACCAAAATCGAGGCATAGGTCAAGAAGTCGCAGTCCTCGGAGAAGGTACCGTGATGACTATAGAGATCGGGACTACAGGAGGAGAAGTCGCAGTAGGAGTTGGGATAGGTATGAGCGTGACAGATACCACCATGGTAGGGACAGATACCGCCATAGACGAAGCAGGAGCCCTAGTGCCAGTCCTGATTACTACAGGAGCAGGGGAAGAGGACGCTATGATGATGATGATGATGATTATGATAGGCGGAGGCGGAGTCGGAGTCGGAGCCATTCTTTTGATAGTGCATCCCCTGCTCGGCGTAGCCTTACACCTCAAAGGAGCCTTTCTCCGTTAAGCAGAAGCCGCAGTGTCAGCCCTTATGGACATGGTCGTGATGGACGATCGCCTAATTCTCAAAGTGTTTCACCACGAGGTCGTCCTGCTGATTCTCGAAGTCCATCCCCTCGACATTCAGATGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04122 map04122
Pfam Domains
Protein Families
Urm1

Protein Analysis

264

Amino Acids

31.22

Weight (kDa)

10.93

Isoelectric Point (pI)

97.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 18 - 88 4.9e-19 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38180
fragaria_vesca FvH4_1g09751 FvH4_1g09751 FvH4_1g09751 FvH4_1g12531 FvH4_1g19193 FvH4_2g07051 FvH4_2g17131 FvH4_2g17131 FvH4_2g28082 FvH4_5g08845 FvH4_5g26361 FvH4_5g26361 FvH4_5g26361 FvH4_5g26361 FvH4_5g39269 FvH4_6g36651 FvH4_6g38410 FvH4_7g04941 FvH4_7g04941
malus_domestica MD00G1156400.v1.1 MD02G1139600.v1.1 MD05G1144600.v1.1 MD06G1002400.v1.1 MD10G1143900.v1.1 MD15G1227300.v1.1 MD15G1252700.v1.1
prunus_persica Prupe.2G147900_v2.0.a1 Prupe.3G257900_v2.0.a1 Prupe.5G003600_v2.0.a1 Prupe.5G053800_v2.0.a1 Prupe.6G011300_v2.0.a1 Prupe.7G161400_v2.0.a1 Prupe.7G161500_v2.0.a1 Prupe.7G188000_v2.0.a1 Prupe.8G182700_v2.0.a1 Prupe.8G182700_v2.0.a1 Prupe.8G182700_v2.0.a1
pyrus_communis pycom02g11030 pycom05g13970 pycom06g00250 pycom10g12390 pycom10g12400 pycom15g20220 pycom15g22260 pycom17g05620
rosa_chinensis RchiOBHm_Chr2g0096701 RchiOBHm_Chr2g0100471 RchiOBHm_Chr2g0100481 RchiOBHm_Chr6g0281561 RchiOBHm_Chr7g0187081 RchiOBHm_Chr7g0217201 RchiOBHm_Chr7g0228321
rosa_laevigata RLG00000001574 RLG00000002527 RLG00000008169 RLG00000011104 RLG00000012963 RLG00000013720 RLG00000014615 RLG00000016663 RLG00000018214 RLG00000018621 RLG00000018622 RLG00000022494
rosa_multiflora Rmu_co8187762.1_g000001 Rmu_co8276569.1_g000001 Rmu_sc0000528.1_g000025 Rmu_sc0001095.1_g000001 Rmu_sc0001095.1_g000002 Rmu_sc0001167.1_g000007 Rmu_sc0001758.1_g000042 Rmu_sc0002147.1_g000004 Rmu_sc0002489.1_g000048 Rmu_sc0002564.1_g000015 Rmu_sc0002902.1_g000037 Rmu_sc0003565.1_g000022 Rmu_sc0003576.1_g000008 Rmu_sc0003720.1_g000022 Rmu_sc0003720.1_g000023 Rmu_sc0003862.1_g000023 Rmu_sc0006475.1_g000009 Rmu_sc0006475.1_g000010 Rmu_sc0006735.1_g000001 Rmu_sc0007586.1_g000004 Rmu_sc0009650.1_g000010 Rmu_sc0010963.1_g000005 Rmu_sc0012557.1_g000002 Rmu_sc0020744.1_g000001 Rmu_sc0020745.1_g000002 Rmu_sc0022201.1_g000001 Rmu_sc0022408.1_g000011 Rmu_sc0023414.1_g000001 Rmu_sc0025101.1_g000001 Rmu_ssc0000211.1_g000015 Rmu_ssc0000240.1_g000012
rosa_roxburghii Rroxscaffold_1G00013940 Rroxscaffold_1G00018420 Rroxscaffold_2G00142210 Rroxscaffold_2G00142230 Rroxscaffold_2G00145400 Rroxscaffold_3G00240120 Rroxscaffold_3G00240150 Rroxscaffold_3G00242500 Rroxscaffold_5G00346610 Rroxscaffold_5G00356000 Rroxscaffold_7G00187000 Rroxscaffold_7G00212120
rosa_rugosa Rorug01G0260900 Rorug01G0446900 Rorug02G0059800 Rorug02G0059900 Rorug02G0088400 Rorug02G0088500 Rorug02G0522300 Rorug03G0234100 Rorug03G0234100 Rorug03G0242400 Rorug04G0109900 Rorug04G0326100 Rorug05G0275900 Rorug06G0141600 Rorug06G0141700 Rorug06G0141700 Rorug07G0167200 Rorug07G0247000 Rorug07G0247000 Rorug07G0247100.1
rosa_samantha Rh1AG046800 Rh1AG111600 Rh1AG242600 Rh1CG226400 Rh1DG086400 Rh2AG107200 Rh2AG138100 Rh2AG528600 Rh2BG109600 Rh2BG141200 Rh2BG273400 Rh2BG357800 Rh2BG640200 Rh2CG111400 Rh2CG143400 Rh2CG161100 Rh2CG251300 Rh2DG111000 Rh2DG142900 Rh2DG651100 Rh3BG318000 Rh4AG167800 Rh4AG253700 Rh5AG282900 Rh6AG254100 Rh6BG257100 Rh6CG256400 Rh6DG248200 Rh7AG306100 Rh7BG297500 Rh7CG325200 Rh7CG420200 Rh7DG306000
rosa_wichuraiana Rw2G008310 Rw2G010790 Rw2G010800 Rw2G013840 Rw2G022430 Rw3G020550 Rw3G024980 Rw3G025930 Rw5G026810 Rw5G035870 Rw6G022060 Rw6G030700 Rw7G007420 Rw7G026010 Rw7G033340 Rw7G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 163
Acc65I GGTACC 1 cut(s) 375
AccB1I GGYRCC 1 cut(s) 375
AccI GTMKAC 1 cut(s) 129
AccII CGCG 1 cut(s) 255
AccIII TCCGGA 2 cut(s) 28, 75
AciI CCGC 6 cut(s) 86, 101, 478, 574, 580, 673
AfaI GTAC 2 cut(s) 116, 377
AfiI CCNNNNNNNGG 2 cut(s) 466, 624
AjnI CCWGG 1 cut(s) 167
Alw26I GTCTC 2 cut(s) 9, 141
Aor13HI TCCGGA 2 cut(s) 28, 75
AoxI GGCC 2 cut(s) 22, 284
Asp718I GGTACC 1 cut(s) 375
AspS9I GGNCC 2 cut(s) 23, 284
AsuHPI GGTGA 3 cut(s) 28, 61, 726
BanI GGYRCC 1 cut(s) 375
BanII GRGCYC 1 cut(s) 500
BauI CACGAG 1 cut(s) 738
BccI CCATC 4 cut(s) 227, 242, 698, 775
BceAI ACGGC 1 cut(s) 133
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BciT130I CCWGG 1 cut(s) 169
BcoDI GTCTC 2 cut(s) 9, 141
BfaI CTAG 1 cut(s) 501
BfmI CTRYAG 3 cut(s) 388, 403, 520
BisI GCNGC 1 cut(s) 673
BlsI GCNGC 1 cut(s) 674
Bme1390I CCNGG 1 cut(s) 169
BmgT120I GGNCC 2 cut(s) 23, 284
BmiI GGNNCC 4 cut(s) 377, 497, 596, 650
BmrFI CCNGG 1 cut(s) 169
BmsI GCATC 1 cut(s) 623
BplI GAGNNNNNCTC 2 cut(s) 640, 672
BsaI GGTCTC 1 cut(s) 141
BsaJI CCNNGG 3 cut(s) 168, 365, 460
BsaWI WCCGGW 2 cut(s) 28, 75
BsaXI ACNNNNNCTCC 8 cut(s) 400, 430, 487, 517, 568, 574, 598, 604
Bsc4I CCNNNNNNNGG 2 cut(s) 466, 624
Bse1I ACTGG 2 cut(s) 163, 507
BseAI TCCGGA 2 cut(s) 28, 75
BseBI CCWGG 1 cut(s) 169
BseDI CCNNGG 3 cut(s) 168, 365, 460
BseGI GGATG 4 cut(s) 94, 138, 614, 767
BseLI CCNNNNNNNGG 2 cut(s) 466, 624
BseNI ACTGG 2 cut(s) 163, 507
BseRI GAGGAG 2 cut(s) 44, 424
Bsh1236I CGCG 1 cut(s) 255
Bsh1285I CGRYCG 1 cut(s) 713
BshFI GGCC 2 cut(s) 24, 286
BshNI GGYRCC 1 cut(s) 375
BsiEI CGRYCG 1 cut(s) 713
BsiSI CCGG 2 cut(s) 29, 76
BslFI GGGAC 2 cut(s) 413, 482
BslI CCNNNNNNNGG 2 cut(s) 466, 624
BsmAI GTCTC 2 cut(s) 9, 141
BsmFI GGGAC 2 cut(s) 413, 482
BsnI GGCC 2 cut(s) 24, 286
Bso31I GGTCTC 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 500
Bsp13I TCCGGA 2 cut(s) 28, 75
Bsp143I GATC 3 cut(s) 91, 394, 710
Bsp19I CCATGG 1 cut(s) 460
Bsp68I TCGCGA 1 cut(s) 255
BspACI CCGC 6 cut(s) 86, 101, 478, 574, 580, 673
BspANI GGCC 2 cut(s) 24, 286
BspEI TCCGGA 2 cut(s) 28, 75
BspFNI CGCG 1 cut(s) 255
BspLI GGNNCC 4 cut(s) 377, 497, 596, 650
BspT107I GGYRCC 1 cut(s) 375
BspTNI GGTCTC 1 cut(s) 141
BsrI ACTGG 2 cut(s) 163, 507
BssECI CCNNGG 3 cut(s) 168, 365, 460
BssMI GATC 3 cut(s) 91, 394, 710
BssSI CACGAG 1 cut(s) 738
BssT1I CCWWGG 1 cut(s) 460
Bst2BI CACGAG 1 cut(s) 738
Bst2UI CCWGG 1 cut(s) 169
Bst4CI ACNGT 2 cut(s) 265, 380
Bst6I CTCTTC 2 cut(s) 232, 529
BstAPI GCANNNNNTGC 1 cut(s) 620
BstDSI CCRYGG 1 cut(s) 460
BstF5I GGATG 4 cut(s) 94, 138, 614, 767
BstFNI CGCG 1 cut(s) 255
BstKTI GATC 3 cut(s) 94, 397, 713
BstMAI GTCTC 2 cut(s) 9, 141
BstMBI GATC 3 cut(s) 91, 394, 710
BstMCI CGRYCG 1 cut(s) 713
BstMWI GCNNNNNNNGC 5 cut(s) 206, 215, 620, 672, 681
BstNI CCWGG 1 cut(s) 169
BstSCI CCNGG 1 cut(s) 167
BstSFI CTRYAG 3 cut(s) 388, 403, 520
BstUI CGCG 1 cut(s) 255
BsuRI GGCC 2 cut(s) 24, 286
BtgI CCRYGG 1 cut(s) 460
BtsCI GGATG 4 cut(s) 94, 138, 614, 767
BtsI GCAGTG 1 cut(s) 682
BtsIMutI CAGTG 1 cut(s) 682
BtuMI TCGCGA 1 cut(s) 255
Cfr13I GGNCC 2 cut(s) 23, 284
CseI GACGC 1 cut(s) 549
Csp6I GTAC 2 cut(s) 115, 376
CviAII CATG 2 cut(s) 461, 695
CviJI RGCY 9 cut(s) 24, 229, 286, 498, 597, 633, 651, 672, 684
CviKI_1 RGCY 9 cut(s) 24, 229, 286, 498, 597, 633, 651, 672, 684
CviQI GTAC 2 cut(s) 115, 376
DpnI GATC 3 cut(s) 93, 396, 712
DpnII GATC 3 cut(s) 91, 394, 710
DrdI GACNNNNNNGTC 1 cut(s) 163
DseDI GACNNNNNNGTC 1 cut(s) 163
Eam1104I CTCTTC 2 cut(s) 232, 529
EarI CTCTTC 2 cut(s) 232, 529
EciI GGCGGA 2 cut(s) 589, 595
Eco130I CCWWGG 1 cut(s) 460
Eco24I GRGCYC 1 cut(s) 500
Eco31I GGTCTC 1 cut(s) 141
EcoRII CCWGG 1 cut(s) 167
EcoT14I CCWWGG 1 cut(s) 460
EcoT38I GRGCYC 1 cut(s) 500
ErhI CCWWGG 1 cut(s) 460
FaeI CATG 2 cut(s) 464, 698
FaqI GGGAC 2 cut(s) 413, 482
FatI CATG 2 cut(s) 460, 694
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 1 cut(s) 673
FokI GGATG 4 cut(s) 101, 125, 601, 754
FriOI GRGCYC 1 cut(s) 500
Fsp4HI GCNGC 1 cut(s) 673
FspBI CTAG 1 cut(s) 501
GluI GCNGC 1 cut(s) 673
HaeIII GGCC 2 cut(s) 24, 286
HapII CCGG 2 cut(s) 29, 76
HgaI GACGC 1 cut(s) 549
Hin1II CATG 2 cut(s) 464, 698
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 5 cut(s) 164, 301, 583, 589, 755
HpaII CCGG 2 cut(s) 29, 76
HphI GGTGA 3 cut(s) 28, 61, 726
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 5 cut(s) 14, 369, 588, 594, 784
Hpy188III TCNNGA 8 cut(s) 29, 76, 254, 351, 398, 512, 701, 759
Hpy8I GTNNAC 1 cut(s) 130
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 5 cut(s) 9, 82, 147, 306, 366
HpyCH4III ACNGT 2 cut(s) 265, 380
HpyCH4V TGCA 4 cut(s) 200, 275, 293, 614
HpyF10VI GCNNNNNNNGC 5 cut(s) 206, 215, 620, 672, 681
Hsp92II CATG 2 cut(s) 464, 698
Kpn2I TCCGGA 2 cut(s) 28, 75
KpnI GGTACC 1 cut(s) 379
Kzo9I GATC 3 cut(s) 91, 394, 710
LmnI GCTCC 4 cut(s) 495, 525, 594, 648
LweI GCATC 1 cut(s) 623
MaeI CTAG 1 cut(s) 501
MaeIII GTNAC 1 cut(s) 446
MalI GATC 3 cut(s) 93, 396, 712
MboI GATC 3 cut(s) 91, 394, 710
MboII GAAGA 3 cut(s) 127, 249, 546
MhlI GDGCHC 1 cut(s) 500
MluCI AATT 1 cut(s) 718
MlyI GAGTC 3 cut(s) 173, 592, 598
MmeI TCCRAC 2 cut(s) 566, 572
MroI TCCGGA 2 cut(s) 28, 75
MseI TTAA 1 cut(s) 662
MslI CAYNNNNRTG 2 cut(s) 699, 783
MspI CCGG 2 cut(s) 29, 76
MspR9I CCNGG 1 cut(s) 169
MvaI CCWGG 1 cut(s) 169
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 5 cut(s) 206, 215, 620, 672, 681
NcoI CCATGG 1 cut(s) 460
NdeII GATC 3 cut(s) 91, 394, 710
NlaIII CATG 2 cut(s) 464, 698
NlaIV GGNNCC 4 cut(s) 377, 497, 596, 650
NmeAIII GCCGAG 1 cut(s) 604
NmuCI GTSAC 1 cut(s) 446
NruI TCGCGA 1 cut(s) 255
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 706
PfeI GAWTC 2 cut(s) 301, 755
PflFI GACNNNGTC 1 cut(s) 696
PkrI GCNGC 1 cut(s) 674
Ple19I CGATCG 1 cut(s) 713
PleI GAGTC 3 cut(s) 172, 591, 597
PpsI GAGTC 3 cut(s) 172, 591, 597
Psp6I CCWGG 1 cut(s) 167
PspGI CCWGG 1 cut(s) 167
PspN4I GGNNCC 4 cut(s) 377, 497, 596, 650
PspPI GGNCC 2 cut(s) 23, 284
PsyI GACNNNGTC 1 cut(s) 696
PvuI CGATCG 1 cut(s) 713
RruI TCGCGA 1 cut(s) 255
RsaI GTAC 2 cut(s) 116, 377
RsaNI GTAC 2 cut(s) 115, 376
RseI CAYNNNNRTG 2 cut(s) 699, 783
SalI GTCGAC 1 cut(s) 128
SaqAI TTAA 1 cut(s) 662
SatI GCNGC 1 cut(s) 673
Sau3AI GATC 3 cut(s) 91, 394, 710
Sau96I GGNCC 2 cut(s) 23, 284
SchI GAGTC 3 cut(s) 173, 592, 598
ScrFI CCNGG 1 cut(s) 169
SduI GDGCHC 1 cut(s) 500
SetI ASST 9 cut(s) 20, 47, 74, 126, 350, 377, 440, 643, 745
SfaNI GCATC 1 cut(s) 623
SfcI CTRYAG 3 cut(s) 388, 403, 520
SmiMI CAYNNNNRTG 2 cut(s) 699, 783
Sse9I AATT 1 cut(s) 718
SsiI CCGC 6 cut(s) 86, 101, 478, 574, 580, 673
SspMI CTAG 1 cut(s) 501
StyD4I CCNGG 1 cut(s) 167
StyI CCWWGG 1 cut(s) 460
TaaI ACNGT 2 cut(s) 265, 380
TaqI TCGA 6 cut(s) 108, 129, 231, 338, 760, 775
TaqII GACCGA 1 cut(s) 165
TasI AATT 1 cut(s) 718
TauI GCSGC 1 cut(s) 675
TfiI GAWTC 2 cut(s) 301, 755
Tru1I TTAA 1 cut(s) 662
Tru9I TTAA 1 cut(s) 662
TscAI CASTG 1 cut(s) 682
TseFI GTSAC 1 cut(s) 446
Tsp45I GTSAC 1 cut(s) 446
TspDTI ATGAA 2 cut(s) 127, 293
TspGWI ACGGA 1 cut(s) 648
TspRI CASTG 1 cut(s) 682
Tth111I GACNNNGTC 1 cut(s) 696
XmiI GTMKAC 1 cut(s) 129
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.