Rroxscaffold_3G00242500

Protein FAR1-RELATED SEQUENCE 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000165
Physical Location & Seq
Forward (+)
34815171 .. 34818776
3606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00242500.1

Sequence Viewer

Length: 2298 bp
ATGGACTATAAGCCTCTGACCATTGGGAACGACGTGGTTGAATTTGACCTTGGTGGTTCTGGGGAAGATGACACCATTGCCCTGGACTTAGAACACCCAGATGATGGCTATGATCTTTTCGAAGAAGACGATTTAGTCCAACTTGCCTCTAGTTCCACTAGGTTTGGTGATGATTTGGGTAATGGGGACCCCAATCTTGAACCTTATGAGGGGATGGAATTCGATTCCGAACAGGCCTCTCGCATTTTCTACAATTCTTATGCAAGGAGAGTTGGTTTTAGTACCCGGGTAAGTGTGTACCAGCGGTCACGCCGCGATGGCTCCATCATATGCCGGCAGATTGTGTGTTCCCGGGAAGGGTTTCGGCGTGATGGAGGTGAAAATAGGTCTAAAAGGCAGCGGACAATCACTAGAGTTGGCTGCAAGGCACAGATGACTGTGAAGAAACAGAGTTCAGGAAGATGGGCTGTTTCGAAATTAGTGAAGGAACATAACCATGAGCTTGTACCGCCGGATAAGGTGCATTGTCTCCGTTCGCATAGGCATGTCTCTGGTCCTGCTCGGAGTCTGATTGATACCCTTCAAGCGGCCGGGATGGGTCCCAGTGGGGTGATGTCTGTGCTGATCAAGGAATCAGGGGGAATTAATAATGTTGGCTTCACGAAAGTTGATTGCCAGAATTACATGAGCAGTAGCAGGCAGAGAACTCTTGGGAGTGGGGGTCAGCTTGTTTTTGACTATTTGAAGCGAATGCAGGATGAGGATCCTGCTTTCTTTTGTGCCGTCCAGGGTGATTTTGAGAACTCAACAGGTAACATATTCTGGGCTGATTCAAATTCAAGAATGAACTATAGTTACTTTGGAGACACTGTTACATTTGACACAACATATAGAACAAATCGATACCGGGTTCCATTTGCTCCATTTACTGGGTGGAATCATCATGGACAGCCTGTGTTATTTGGATGTGCATTACTCCTTAATGAGTCAGAGTCCTCGTTTGTTTGGCTATTCCAGACTTGGTTAGCTGCTATGTCTGGCTGCCAACCTGTCTCAATCACAACAGACCAGGATAGAATCATCAGGGCTGCTGTTGTTCAAGTATTTCCTGGAACCCGCCACCGCTTTTGTAAATGGAATGTGTTCAGGGAAGCTCAGGAGAAGTTGTCCCACATTTATCAGTCACACCCGACCTTTGAAGCCGAGTTCCTGAGGTGCATTAATGCAACAGAGACAATTGATGAATTTGAGATATGTTGGGAGTCCCTTCTTAAAAGGTTTGATCTTTATAGTAATGAATGGCTTCAGTCAATGTACCATGCTCGCCAGCAATGGGTGCCAGTTTTCCTTCGTGACACATTTTTTGGGGAGATGTTTGTAACCCAAGGAAGTGATAATATAAACTCATACTTTGATGGCTATATAAATGCATCCACGAATATTCAGGTGCTGATAAAGCAGTATGAAAAAGCAATTGCCACTCGCCATGAAAAGGAAGTAAAGGCTGACTATGATACATTGAACATCTCTCCAATTCTAAGGACTCCATCTCCTATGGAAAAGCAAGCTGCAAAACTTTATACGAGGACAATATTCATGAAGTTCCAAGAAGAATTGGTCGAAACACTAGCTTATCCGGCAACTGTGGTCGATGATACAGGATCAGAAACCATGTTTCGGGTTGCAAAATTTGGGGAAGACCACAAAGGGCACTTTGTTAAGTTCAATGTTTTTGAGAAGAAAGCTAGTTGTAGCTGCCAAATGTTTGAGTTTTCAGGTATTATTTGCAGGCACATATTAGCAGTTTTCAGAGTAACCAATGTTCTTACACTTCCGTCCCACTATATATTGAAACGTTGGACCAGAAATGCCAAAAGTGGAGTTCTGTTGGATGAGCATGCTCTTGGATTGCCAAATGATTCACAAGATTCCTCTGCTGCTCGGTATGACAATCTACGAAGGGAAGCTATCAAATATGTAGAAGAAGGGGCAGAATCTGAGTGTGTTTATAATGTGGCAATGGACGCTCTTCGTGAGGCTGCGAACAAGGTTGCTGCTGCGAAGAAGCATGGTCCCAGAGTTGTACAACATACTCCAAAGAATTGGAGCCAGCAGCTTCTGTCTTGTACTGTGGATCAGGACAAGAAAATTGAGGAATTGGCAGCTGAGTTGGAAATTGCTATTCAGCGTTGTGAAGCATATCAAGCAAAACTACTTACTATTTTGAAGGACATGGAAGAGCAGAAGTTAAAGATATCAGTGAATGTTCGGAATGTAAGGCTAAATCTCAAAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1 MULE SWIM

Protein Analysis

765

Amino Acids

87.28

Weight (kDa)

6.24

Isoelectric Point (pI)

50.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 83 - 170 5.4e-31 FAR1 DNA-binding domain
ZSWIM1-3_RNaseH-like PF21056 254 - 373 9.1e-06 Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE PF10551 291 - 382 3.5e-28 MULE transposase domain
SWIM PF04434 581 - 604 5.8e-10 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38180
fragaria_vesca FvH4_1g09751 FvH4_1g09751 FvH4_1g09751 FvH4_1g12531 FvH4_1g19193 FvH4_2g07051 FvH4_2g17131 FvH4_2g17131 FvH4_2g28082 FvH4_5g08845 FvH4_5g26361 FvH4_5g26361 FvH4_5g26361 FvH4_5g26361 FvH4_5g39269 FvH4_6g36651 FvH4_6g38410 FvH4_7g04941 FvH4_7g04941
malus_domestica MD00G1156400.v1.1 MD02G1139600.v1.1 MD05G1144600.v1.1 MD06G1002400.v1.1 MD10G1143900.v1.1 MD15G1227300.v1.1 MD15G1252700.v1.1
prunus_persica Prupe.2G147900_v2.0.a1 Prupe.3G257900_v2.0.a1 Prupe.5G003600_v2.0.a1 Prupe.5G053800_v2.0.a1 Prupe.6G011300_v2.0.a1 Prupe.7G161400_v2.0.a1 Prupe.7G161500_v2.0.a1 Prupe.7G188000_v2.0.a1 Prupe.8G182700_v2.0.a1 Prupe.8G182700_v2.0.a1 Prupe.8G182700_v2.0.a1
pyrus_communis pycom02g11030 pycom05g13970 pycom06g00250 pycom10g12390 pycom10g12400 pycom15g20220 pycom15g22260 pycom17g05620
rosa_chinensis RchiOBHm_Chr2g0096701 RchiOBHm_Chr2g0100471 RchiOBHm_Chr2g0100481 RchiOBHm_Chr6g0281561 RchiOBHm_Chr7g0187081 RchiOBHm_Chr7g0217201 RchiOBHm_Chr7g0228321
rosa_laevigata RLG00000001574 RLG00000002527 RLG00000008169 RLG00000011104 RLG00000012963 RLG00000013720 RLG00000014615 RLG00000016663 RLG00000018214 RLG00000018621 RLG00000018622 RLG00000022494
rosa_multiflora Rmu_co8187762.1_g000001 Rmu_co8276569.1_g000001 Rmu_sc0000528.1_g000025 Rmu_sc0001095.1_g000001 Rmu_sc0001095.1_g000002 Rmu_sc0001167.1_g000007 Rmu_sc0001758.1_g000042 Rmu_sc0002147.1_g000004 Rmu_sc0002489.1_g000048 Rmu_sc0002564.1_g000015 Rmu_sc0002902.1_g000037 Rmu_sc0003565.1_g000022 Rmu_sc0003576.1_g000008 Rmu_sc0003720.1_g000022 Rmu_sc0003720.1_g000023 Rmu_sc0003862.1_g000023 Rmu_sc0006475.1_g000009 Rmu_sc0006475.1_g000010 Rmu_sc0006735.1_g000001 Rmu_sc0007586.1_g000004 Rmu_sc0009650.1_g000010 Rmu_sc0010963.1_g000005 Rmu_sc0012557.1_g000002 Rmu_sc0020744.1_g000001 Rmu_sc0020745.1_g000002 Rmu_sc0022201.1_g000001 Rmu_sc0022408.1_g000011 Rmu_sc0023414.1_g000001 Rmu_sc0025101.1_g000001 Rmu_ssc0000211.1_g000015 Rmu_ssc0000240.1_g000012
rosa_roxburghii Rroxscaffold_1G00013940 Rroxscaffold_1G00018420 Rroxscaffold_2G00142210 Rroxscaffold_2G00142230 Rroxscaffold_2G00145400 Rroxscaffold_3G00240120 Rroxscaffold_3G00240150 Rroxscaffold_3G00242500 Rroxscaffold_5G00346610 Rroxscaffold_5G00356000 Rroxscaffold_7G00187000 Rroxscaffold_7G00212120
rosa_rugosa Rorug01G0260900 Rorug01G0446900 Rorug02G0059800 Rorug02G0059900 Rorug02G0088400 Rorug02G0088500 Rorug02G0522300 Rorug03G0234100 Rorug03G0234100 Rorug03G0242400 Rorug04G0109900 Rorug04G0326100 Rorug05G0275900 Rorug06G0141600 Rorug06G0141700 Rorug06G0141700 Rorug07G0167200 Rorug07G0247000 Rorug07G0247000 Rorug07G0247100.1
rosa_samantha Rh1AG046800 Rh1AG111600 Rh1AG242600 Rh1CG226400 Rh1DG086400 Rh2AG107200 Rh2AG138100 Rh2AG528600 Rh2BG109600 Rh2BG141200 Rh2BG273400 Rh2BG357800 Rh2BG640200 Rh2CG111400 Rh2CG143400 Rh2CG161100 Rh2CG251300 Rh2DG111000 Rh2DG142900 Rh2DG651100 Rh3BG318000 Rh4AG167800 Rh4AG253700 Rh5AG282900 Rh6AG254100 Rh6BG257100 Rh6CG256400 Rh6DG248200 Rh7AG306100 Rh7BG297500 Rh7CG325200 Rh7CG420200 Rh7DG306000
rosa_wichuraiana Rw2G008310 Rw2G010790 Rw2G010800 Rw2G013840 Rw2G022430 Rw3G020550 Rw3G024980 Rw3G025930 Rw5G026810 Rw5G035870 Rw6G022060 Rw6G030700 Rw7G007420 Rw7G026010 Rw7G033340 Rw7G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2010
AasI GACNNNNNNGTC 1 cut(s) 134
AccB1I GGYRCC 1 cut(s) 1336
AccB7I CCANNNNNTGG 2 cut(s) 104, 929
AccII CGCG 1 cut(s) 315
AciI CCGC 7 cut(s) 304, 313, 400, 509, 587, 1117, 1123
AclI AACGTT 1 cut(s) 1855
AclWI GGATC 4 cut(s) 758, 771, 1669, 2142
AcoI YGGCCR 1 cut(s) 588
AcsI RAATTY 5 cut(s) 41, 218, 835, 1244, 1688
AcuI CTGAAG 1 cut(s) 1289
AfaI GTAC 6 cut(s) 283, 299, 507, 1316, 2085, 2128
AfiI CCNNNNNNNGG 8 cut(s) 104, 209, 357, 586, 929, 1333, 1492, 1677
AjiI CACGTC 1 cut(s) 34
AjnI CCWGG 4 cut(s) 81, 786, 1068, 1108
AjuI GAANNNNNNNTTGG 2 cut(s) 33, 65
Alw26I GTCTC 5 cut(s) 533, 553, 858, 1057, 1226
AlwI GGATC 4 cut(s) 758, 771, 1669, 2142
AlwNI CAGNNNCTG 3 cut(s) 1450, 1997, 2119
Ama87I CYCGRG 2 cut(s) 285, 351
AoxI GGCC 2 cut(s) 234, 588
ApoI RAATTY 5 cut(s) 41, 218, 835, 1244, 1688
ArsI GACNNNNNNTTYG 2 cut(s) 1346, 1378
AseI ATTAAT 2 cut(s) 645, 1221
Asp700I GAANNNNTTC 3 cut(s) 360, 1142, 1302
AspS9I GGNCC 5 cut(s) 187, 554, 599, 1860, 2072
AsuC2I CCSGG 6 cut(s) 286, 287, 352, 353, 592, 908
AsuHPI GGTGA 4 cut(s) 179, 389, 622, 803
AsuII TTCGAA 2 cut(s) 120, 473
AvaI CYCGRG 2 cut(s) 285, 351
AvaII GGWCC 5 cut(s) 187, 554, 599, 1860, 2072
AxyI CCTNAGG 1 cut(s) 1211
BaeGI GKGCMC 1 cut(s) 1713
BamHI GGATCC 1 cut(s) 763
BanI GGYRCC 1 cut(s) 1336
BbsI GAAGAC 2 cut(s) 132, 1704
BccI CCATC 9 cut(s) 98, 208, 311, 332, 365, 456, 589, 1409, 1555
BceAI ACGGC 1 cut(s) 767
BciT130I CCWGG 4 cut(s) 83, 788, 1070, 1110
BclI TGATCA 1 cut(s) 624
BcnI CCSGG 6 cut(s) 286, 287, 352, 353, 592, 908
BcoDI GTCTC 5 cut(s) 533, 553, 858, 1057, 1226
BfaI CTAG 5 cut(s) 150, 159, 411, 1628, 1746
BfmI CTRYAG 1 cut(s) 850
BglI GCCNNNNNGGC 1 cut(s) 318
Bme18I GGWCC 5 cut(s) 187, 554, 599, 1860, 2072
BmeT110I CYCGRG 2 cut(s) 285, 351
BmgBI CACGTC 1 cut(s) 34
BmgT120I GGNCC 5 cut(s) 187, 554, 599, 1860, 2072
BmrI ACTGGG 2 cut(s) 597, 939
BmsI GCATC 1 cut(s) 1439
BmuI ACTGGG 2 cut(s) 597, 939
BpiI GAAGAC 2 cut(s) 132, 1704
Bpu10I CCTNAGC 1 cut(s) 1155
Bpu14I TTCGAA 2 cut(s) 120, 473
BpuMI CCSGG 6 cut(s) 286, 287, 352, 353, 592, 908
Bsa29I ATCGAT 1 cut(s) 901
BsaBI GATNNNNATC 1 cut(s) 762
BsaJI CCNNGG 6 cut(s) 49, 81, 285, 351, 787, 1384
Bsc4I CCNNNNNNNGG 8 cut(s) 104, 209, 357, 586, 929, 1333, 1492, 1677
Bse118I RCCGGY 1 cut(s) 333
Bse1I ACTGG 3 cut(s) 603, 934, 1340
Bse21I CCTNAGG 1 cut(s) 1211
Bse3DI GCAATG 3 cut(s) 75, 1337, 2025
Bse8I GATNNNNATC 1 cut(s) 762
BseBI CCWGG 4 cut(s) 83, 788, 1070, 1110
BseCI ATCGAT 1 cut(s) 901
BseDI CCNNGG 6 cut(s) 49, 81, 285, 351, 787, 1384
BseGI GGATG 6 cut(s) 219, 600, 763, 971, 1430, 1897
BseJI GATNNNNATC 1 cut(s) 762
BseLI CCNNNNNNNGG 8 cut(s) 104, 209, 357, 586, 929, 1333, 1492, 1677
BseMI GCAATG 3 cut(s) 75, 1337, 2025
BseMII CTCAG 4 cut(s) 1169, 1202, 1989, 2157
BseNI ACTGG 3 cut(s) 603, 934, 1340
BseSI GKGCMC 1 cut(s) 1713
BseX3I CGGCCG 1 cut(s) 588
Bsh1236I CGCG 1 cut(s) 315
Bsh1285I CGRYCG 1 cut(s) 591
BshFI GGCC 2 cut(s) 236, 590
BshNI GGYRCC 1 cut(s) 1336
BshVI ATCGAT 1 cut(s) 901
BsiEI CGRYCG 1 cut(s) 591
BsiHKCI CYCGRG 2 cut(s) 285, 351
BsiSI CCGG 7 cut(s) 286, 334, 352, 512, 591, 907, 1637
BslFI GGGAC 6 cut(s) 200, 585, 1153, 1249, 1822, 2058
BslI CCNNNNNNNGG 8 cut(s) 104, 209, 357, 586, 929, 1333, 1492, 1677
BsmAI GTCTC 5 cut(s) 533, 553, 858, 1057, 1226
BsmFI GGGAC 6 cut(s) 200, 585, 1153, 1249, 1822, 2058
BsmI GAATGC 1 cut(s) 756
BsnI GGCC 2 cut(s) 236, 590
BsoBI CYCGRG 2 cut(s) 285, 351
Bsp119I TTCGAA 2 cut(s) 120, 473
Bsp1286I GDGCHC 1 cut(s) 1713
Bsp1407I TGTACA 1 cut(s) 2083
Bsp143I GATC 6 cut(s) 112, 624, 763, 1282, 1661, 2134
BspACI CCGC 7 cut(s) 304, 313, 400, 509, 587, 1117, 1123
BspANI GGCC 2 cut(s) 236, 590
BspCNI CTCAG 4 cut(s) 1168, 1203, 1990, 2158
BspDI ATCGAT 1 cut(s) 901
BspFNI CGCG 1 cut(s) 315
BspHI TCATGA 1 cut(s) 1596
BspPI GGATC 4 cut(s) 758, 771, 1669, 2142
BspQI GCTCTTC 2 cut(s) 2034, 2232
BspT104I TTCGAA 2 cut(s) 120, 473
BspT107I GGYRCC 1 cut(s) 1336
BsrDI GCAATG 3 cut(s) 75, 1337, 2025
BsrFI RCCGGY 1 cut(s) 333
BsrGI TGTACA 1 cut(s) 2083
BsrI ACTGG 3 cut(s) 603, 934, 1340
BssAI RCCGGY 1 cut(s) 333
BssECI CCNNGG 6 cut(s) 49, 81, 285, 351, 787, 1384
BssMI GATC 6 cut(s) 112, 624, 763, 1282, 1661, 2134
BssT1I CCWWGG 2 cut(s) 49, 1384
Bst2UI CCWGG 4 cut(s) 83, 788, 1070, 1110
Bst4CI ACNGT 4 cut(s) 439, 871, 1645, 2131
Bst6I CTCTTC 2 cut(s) 2034, 2232
BstAPI GCANNNNNTGC 1 cut(s) 1336
BstAUI TGTACA 1 cut(s) 2083
BstBI TTCGAA 2 cut(s) 120, 473
BstC8I GCNNGC 8 cut(s) 335, 698, 1324, 1328, 1566, 1790, 1899, 2111
BstDEI CTNAG 6 cut(s) 88, 1155, 1211, 1538, 1998, 2166
BstF5I GGATG 6 cut(s) 219, 600, 763, 971, 1430, 1897
BstFNI CGCG 1 cut(s) 315
BstKTI GATC 6 cut(s) 115, 627, 766, 1285, 1664, 2137
BstMAI GTCTC 5 cut(s) 533, 553, 858, 1057, 1226
BstMBI GATC 6 cut(s) 112, 624, 763, 1282, 1661, 2134
BstMCI CGRYCG 1 cut(s) 591
BstMWI GCNNNNNNNGC 7 cut(s) 318, 508, 1336, 1456, 1637, 2024, 2204
BstNI CCWGG 4 cut(s) 83, 788, 1070, 1110
BstNSI RCATGY 2 cut(s) 548, 1901
BstSFI CTRYAG 1 cut(s) 850
BstSLI GKGCMC 1 cut(s) 1713
BstUI CGCG 1 cut(s) 315
BstV2I GAAGAC 2 cut(s) 132, 1704
BstX2I RGATCY 1 cut(s) 763
BstXI CCANNNNNNTGG 2 cut(s) 82, 2103
BstYI RGATCY 1 cut(s) 763
BstZI CGGCCG 1 cut(s) 588
Bsu15I ATCGAT 1 cut(s) 901
Bsu36I CCTNAGG 1 cut(s) 1211
BsuRI GGCC 2 cut(s) 236, 590
BsuTUI ATCGAT 1 cut(s) 901
BtgZI GCGATG 1 cut(s) 330
BtrI CACGTC 1 cut(s) 34
BtsCI GGATG 6 cut(s) 219, 600, 763, 971, 1430, 1897
BtsIMutI CAGTG 3 cut(s) 610, 867, 2265
Cac8I GCNNGC 8 cut(s) 335, 698, 1324, 1328, 1566, 1790, 1899, 2111
CaiI CAGNNNCTG 3 cut(s) 1450, 1997, 2119
CciI TCATGA 1 cut(s) 1596
Cfr10I RCCGGY 1 cut(s) 333
Cfr13I GGNCC 5 cut(s) 187, 554, 599, 1860, 2072
Cfr9I CCCGGG 2 cut(s) 285, 351
ClaI ATCGAT 1 cut(s) 901
CseI GACGC 1 cut(s) 2033
Csp6I GTAC 6 cut(s) 282, 298, 506, 1315, 2084, 2127
CspCI CAANNNNNGTGG 4 cut(s) 145, 180, 1109, 1144
CviQI GTAC 6 cut(s) 282, 298, 506, 1315, 2084, 2127
DdeI CTNAG 6 cut(s) 88, 1155, 1211, 1538, 1998, 2166
DpnI GATC 6 cut(s) 114, 626, 765, 1284, 1663, 2136
DpnII GATC 6 cut(s) 112, 624, 763, 1282, 1661, 2134
DrdI GACNNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 134
EaeI YGGCCR 1 cut(s) 588
EagI CGGCCG 1 cut(s) 588
Eam1104I CTCTTC 2 cut(s) 2034, 2232
EarI CTCTTC 2 cut(s) 2034, 2232
EclXI CGGCCG 1 cut(s) 588
Eco130I CCWWGG 2 cut(s) 49, 1384
Eco147I AGGCCT 1 cut(s) 236
Eco32I GATATC 1 cut(s) 2256
Eco47I GGWCC 5 cut(s) 187, 554, 599, 1860, 2072
Eco52I CGGCCG 1 cut(s) 588
Eco57I CTGAAG 1 cut(s) 1289
Eco81I CCTNAGG 1 cut(s) 1211
Eco88I CYCGRG 2 cut(s) 285, 351
EcoO109I RGGNCCY 2 cut(s) 187, 599
EcoRI GAATTC 1 cut(s) 218
EcoRII CCWGG 4 cut(s) 81, 786, 1068, 1108
EcoRV GATATC 1 cut(s) 2256
EcoT14I CCWWGG 2 cut(s) 49, 1384
EcoT22I ATGCAT 1 cut(s) 1432
ErhI CCWWGG 2 cut(s) 49, 1384
FaqI GGGAC 6 cut(s) 200, 585, 1153, 1249, 1822, 2058
FauI CCCGC 1 cut(s) 1124
FauNDI CATATG 1 cut(s) 329
FbaI TGATCA 1 cut(s) 624
FokI GGATG 6 cut(s) 226, 607, 770, 978, 1417, 1904
FspBI CTAG 5 cut(s) 150, 159, 411, 1628, 1746
HaeIII GGCC 2 cut(s) 236, 590
HapII CCGG 7 cut(s) 286, 334, 352, 512, 591, 907, 1637
HgaI GACGC 1 cut(s) 2033
HpaII CCGG 7 cut(s) 286, 334, 352, 512, 591, 907, 1637
HphI GGTGA 4 cut(s) 179, 389, 622, 803
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 9 cut(s) 18, 229, 564, 570, 991, 1666, 1811, 1999, 2271
Hpy8I GTNNAC 1 cut(s) 298
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 8 cut(s) 350, 478, 590, 1277, 1358, 1953, 1979, 2221
HpyCH4III ACNGT 4 cut(s) 439, 871, 1645, 2131
HpyCH4IV ACGT 2 cut(s) 33, 1855
HpyF10VI GCNNNNNNNGC 7 cut(s) 318, 508, 1336, 1456, 1637, 2024, 2204
HpyF3I CTNAG 6 cut(s) 88, 1155, 1211, 1538, 1998, 2166
HpySE526I ACGT 2 cut(s) 33, 1855
KflI GGGWCCC 2 cut(s) 187, 599
KroI GCCGGC 1 cut(s) 333
KroNI GCCGGC 1 cut(s) 335
Ksp22I TGATCA 1 cut(s) 624
Kzo9I GATC 6 cut(s) 112, 624, 763, 1282, 1661, 2134
LguI GCTCTTC 2 cut(s) 2034, 2232
LmnI GCTCC 3 cut(s) 326, 925, 2106
LweI GCATC 1 cut(s) 1439
MaeI CTAG 5 cut(s) 150, 159, 411, 1628, 1746
MaeII ACGT 2 cut(s) 33, 1855
MaeIII GTNAC 8 cut(s) 306, 812, 854, 871, 1182, 1352, 1378, 1813
MalI GATC 6 cut(s) 114, 626, 765, 1284, 1663, 2136
MboI GATC 6 cut(s) 112, 624, 763, 1282, 1661, 2134
MfeI CAATTG 2 cut(s) 1236, 1473
MflI RGATCY 1 cut(s) 763
MhlI GDGCHC 1 cut(s) 1713
MlyI GAGTC 5 cut(s) 574, 995, 1001, 1271, 1537
MmeI TCCRAC 4 cut(s) 163, 1838, 1869, 2151
Mph1103I ATGCAT 1 cut(s) 1432
MroNI GCCGGC 1 cut(s) 333
MroXI GAANNNNTTC 3 cut(s) 360, 1142, 1302
MseI TTAA 6 cut(s) 645, 981, 1221, 1272, 1719, 2249
MslI CAYNNNNRTG 3 cut(s) 99, 495, 543
MspA1I CMGCKG 3 cut(s) 304, 400, 2165
MspI CCGG 7 cut(s) 286, 334, 352, 512, 591, 907, 1637
MunI CAATTG 2 cut(s) 1236, 1473
Mva1269I GAATGC 1 cut(s) 756
MvaI CCWGG 4 cut(s) 83, 788, 1070, 1110
MvnI CGCG 1 cut(s) 315
MwoI GCNNNNNNNGC 7 cut(s) 318, 508, 1336, 1456, 1637, 2024, 2204
NaeI GCCGGC 1 cut(s) 335
NciI CCSGG 6 cut(s) 286, 287, 352, 353, 592, 908
NdeI CATATG 1 cut(s) 329
NdeII GATC 6 cut(s) 112, 624, 763, 1282, 1661, 2134
NgoMIV GCCGGC 1 cut(s) 333
NmeAIII GCCGAG 1 cut(s) 1228
NmuCI GTSAC 3 cut(s) 306, 1182, 1352
NsiI ATGCAT 1 cut(s) 1432
NspI RCATGY 2 cut(s) 548, 1901
NspV TTCGAA 2 cut(s) 120, 473
PaeI GCATGC 1 cut(s) 1901
PagI TCATGA 1 cut(s) 1596
PceI AGGCCT 1 cut(s) 236
PciSI GCTCTTC 2 cut(s) 2034, 2232
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PctI GAATGC 1 cut(s) 756
PdiI GCCGGC 1 cut(s) 335
PdmI GAANNNNTTC 3 cut(s) 360, 1142, 1302
PfeI GAWTC 8 cut(s) 224, 632, 830, 937, 1077, 1919, 1928, 1994
PflMI CCANNNNNTGG 2 cut(s) 104, 929
PfoI TCCNGGA 1 cut(s) 1108
PleI GAGTC 5 cut(s) 573, 994, 1000, 1270, 1537
PpsI GAGTC 5 cut(s) 573, 994, 1000, 1270, 1537
PpuMI RGGWCCY 2 cut(s) 187, 599
PshBI ATTAAT 2 cut(s) 645, 1221
PsiI TTATAA 1 cut(s) 2010
Psp1406I AACGTT 1 cut(s) 1855
Psp5II RGGWCCY 2 cut(s) 187, 599
Psp6I CCWGG 4 cut(s) 81, 786, 1068, 1108
PspGI CCWGG 4 cut(s) 81, 786, 1068, 1108
PspPI GGNCC 5 cut(s) 187, 554, 599, 1860, 2072
PspPPI RGGWCCY 2 cut(s) 187, 599
PsrI GAACNNNNNNTAC 4 cut(s) 839, 871, 1806, 1838
PstNI CAGNNNCTG 3 cut(s) 1450, 1997, 2119
PsuI RGATCY 1 cut(s) 763
PvuII CAGCTG 1 cut(s) 2165
RsaI GTAC 6 cut(s) 283, 299, 507, 1316, 2085, 2128
RsaNI GTAC 6 cut(s) 282, 298, 506, 1315, 2084, 2127
RseI CAYNNNNRTG 3 cut(s) 99, 495, 543
SapI GCTCTTC 2 cut(s) 2034, 2232
SaqAI TTAA 6 cut(s) 645, 981, 1221, 1272, 1719, 2249
Sau3AI GATC 6 cut(s) 112, 624, 763, 1282, 1661, 2134
Sau96I GGNCC 5 cut(s) 187, 554, 599, 1860, 2072
SchI GAGTC 5 cut(s) 574, 995, 1001, 1271, 1537
SduI GDGCHC 1 cut(s) 1713
SfaNI GCATC 1 cut(s) 1439
SfcI CTRYAG 1 cut(s) 850
SfuI TTCGAA 2 cut(s) 120, 473
SinI GGWCC 5 cut(s) 187, 554, 599, 1860, 2072
SmaI CCCGGG 2 cut(s) 287, 353
SmiMI CAYNNNNRTG 3 cut(s) 99, 495, 543
SphI GCATGC 1 cut(s) 1901
SseBI AGGCCT 1 cut(s) 236
SsiI CCGC 7 cut(s) 304, 313, 400, 509, 587, 1117, 1123
SspI AATATT 2 cut(s) 1441, 1593
SspMI CTAG 5 cut(s) 150, 159, 411, 1628, 1746
StuI AGGCCT 1 cut(s) 236
StyI CCWWGG 2 cut(s) 49, 1384
TaaI ACNGT 4 cut(s) 439, 871, 1645, 2131
TaiI ACGT 2 cut(s) 36, 1858
TaqI TCGA 6 cut(s) 120, 222, 473, 901, 1620, 1650
TatI WGTACW 2 cut(s) 2083, 2126
TauI GCSGC 2 cut(s) 315, 590
TfiI GAWTC 8 cut(s) 224, 632, 830, 937, 1077, 1919, 1928, 1994
Tru1I TTAA 6 cut(s) 645, 981, 1221, 1272, 1719, 2249
Tru9I TTAA 6 cut(s) 645, 981, 1221, 1272, 1719, 2249
TscAI CASTG 3 cut(s) 610, 874, 2265
TseFI GTSAC 3 cut(s) 306, 1182, 1352
Tsp45I GTSAC 3 cut(s) 306, 1182, 1352
TspDTI ATGAA 7 cut(s) 860, 1257, 1311, 1479, 1503, 1585, 1613
TspGWI ACGGA 2 cut(s) 521, 1824
TspMI CCCGGG 2 cut(s) 285, 351
TspRI CASTG 3 cut(s) 610, 874, 2265
Van91I CCANNNNNTGG 2 cut(s) 104, 929
VpaK11BI GGWCC 5 cut(s) 187, 554, 599, 1860, 2072
VspI ATTAAT 2 cut(s) 645, 1221
XapI RAATTY 5 cut(s) 41, 218, 835, 1244, 1688
XceI RCATGY 2 cut(s) 548, 1901
XcmI CCANNNNNNNNNTGG 1 cut(s) 930
XmaI CCCGGG 2 cut(s) 285, 351
XmnI GAANNNNTTC 3 cut(s) 360, 1142, 1302
XspI CTAG 5 cut(s) 150, 159, 411, 1628, 1746
Zsp2I ATGCAT 1 cut(s) 1432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.