MD06G1002400.v1.1

Protein FAR1-RELATED SEQUENCE 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000165
Physical Location & Seq
Forward (+)
247728 .. 251246
3519 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1002400.v1.1.491

Sequence Viewer

Length: 2301 bp
ATGGACTCTGAGCCTCTCAACATTGACTCTGACATCCAAGTGGTGGAATTTGGCGAAGAAGATGACGCCGCCACCGCCACCGCCCTCCACTTACAACACACTGATCATAGCTATGATATTTTCGATGATCACTTGGTTCAACACTCTAATTCTCCTAGGTCTCGTGATGATTTCCCTAATGCGGATGGGGACCCCAGTCTTGAACCCTACGAGGGCATGGAATTCGACTCTGAACAGGCCTCCCGCATTTTCTATAATTCTTATGCCCGTCGAGTTGGTTTCAGCACCCGCGTCAGCGTCTACCAGCGCTCCCGTCGCGATGGCTCCATCATATGCCGCCAAATTGTCTGCTCCCGTGAAGGCTTTCGCCGCGACGGTGGTGAAAATAGGTCCAAGCGCCAGCGTACGGTTACTAGAGTTGGTTGTAAAGCACAGATGACCGTCAAGAAACAGACTTCAGGACGATGGGCTGTTTCAAAGCTGGTAAAGGAACATAACCATGAGCTTGTGCCTCCGGATAGGGTGCATTGTCTCCGTTCGCATAGGCATGTCTCTGGTCCTGCTCGAAGCCTGATTGATACCCTTCAAGCGGCTGGCATGGGTCCTAGTGGAGTGATGTCTGTGCTCATCAAGGAGTCAGGTGGAATTAATAACGTTGGTTTCACAAAAGTTGATTGCCAGAACTATATGAGTAGCAGCAGGCAGAGAACTCTTGGGAGTGGGGGTCAGGTTGTTTTTGACTATTTGAAGCGAATGCAGGTCGAGGATCCTGATTTCTTTTGTGCCGTCCAGGGTGATTTTGAGAACTCGACAGGAAACATTTTCTGGGCTGATGCAAATTCGAGAATGAATTATGACTACTTTGGAGACACTGTAACATTTGACACAACTTATAGGACAAACCGATACCGTGTCCCATTTGCCCCTTTTACTGGATGGAATCATCACGGACAGCCTGTTTTATTTGGATGCGCATTACTCCTTAATGAGTCAGAGTCCTCATTTGTTTGGCTCTTCCAGACTTGGCTAGCTGCAATGTCGAGCCGCCATCCCGTCTCACTCACAACAGACCAGGATAGAATCATTCGGGCTGCTGTTGTGCATGTATTCCCCGGAACTCGCCACCGGTTTTGTAAATGGAACGTATTCAGAGAAGCTCAGGAGAAATTGTCCGACATATACCACTCACACCCCACCTTTGAAGCTGAATTGCTGAAGTGCATCAATGTGACTGAGACAATTGAGGAATTTGAGTCATGTTGGGAGTCCCTTGTTCAAAGATATGATCTTGGGGATAATGAGTGGCTTCAGTCAATATACGATGCTCGGCAACAATGGGTGCCAGTTTTTCTCCAGGATACATTTTTTGGTGAGATGTCAGCAACCCAAGGAAGTGATAATATAAACTCATATTTTGATGGCTATATAAACGCATCAACTAACATTCAGGTGCTGATAAAGCAGTATGAAAAAGCAATTGCCACTCGCCATGAAAAGGAAGTAAAGGCTGATTATGATACAATGAACAGCTCTCCGATTCTGAGGACGCCATCTCCAATGGAAAAACAAGCTGCCAAACTTTATACAAGGATAATATTCATGAAATTCCAAGAGGAATTAGTCGAGACACTTGCTTATCCTGCAACTGTGGTTGATGATACAGGATCAGAAACCATGTATGGGGTGGCAAAATTTGGGGAAGACCACAAGGTGCACTCTGTTAAGTTCAATGTTTTTGAGAAGAAAGCCCGTTGCAGCTGCCAATTGTTTGAGTTTTCAGGCATTATATGTAGGCACATATTAGCAGTCTTCCGAGTAACCAATGTTCTTACGCTTCCATCACACTATATATTAAAACGTTGGACAAAAAATGCCAAAAGTGGAGTTGTATGGGATGAGAACACTCTTGGATTGCCAAATGATTCTCAAGATTCCAGTGCTGCTCGGTATGACAATCTACGCAGGGAAGCAATCAAATATGTAGAAGAAGGGGCTGAATCTGTGCATGTTTATAATGTGGCAATGGATGCTCTTCGCGAAGCTGCAAATGAGGTTGCCGCAGCAAAGAATCATGGTCTTGCAATTGCACAAAATACCCCAGTAAATTGCAGTCAGAAACTTCAGTCTTGTACCATGGATCAGGACAAGAAACTTGAAGAGTTGGCAGCTGCGTTGGAAATTTCAAGTCAGCAATGTGAAGCATTCAGAGAAAAGCTACTCGCTGTTTTGAAAGATATGGAAGAACAGAAGTTAAAGATATCAGTAAATGTTCAGAATGTAAGGCTAAACCTAAAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1 MULE SWIM

Protein Analysis

767

Amino Acids

87.14

Weight (kDa)

5.93

Isoelectric Point (pI)

52.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 84 - 171 5.8e-31 FAR1 DNA-binding domain
MULE PF10551 292 - 383 3.5e-27 MULE transposase domain
SWIM PF04434 582 - 605 9.6e-09 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38180
fragaria_vesca FvH4_1g09751 FvH4_1g09751 FvH4_1g09751 FvH4_1g12531 FvH4_1g19193 FvH4_2g07051 FvH4_2g17131 FvH4_2g17131 FvH4_2g28082 FvH4_5g08845 FvH4_5g26361 FvH4_5g26361 FvH4_5g26361 FvH4_5g26361 FvH4_5g39269 FvH4_6g36651 FvH4_6g38410 FvH4_7g04941 FvH4_7g04941
malus_domestica MD00G1156400.v1.1 MD02G1139600.v1.1 MD05G1144600.v1.1 MD06G1002400.v1.1 MD10G1143900.v1.1 MD15G1227300.v1.1 MD15G1252700.v1.1
prunus_persica Prupe.2G147900_v2.0.a1 Prupe.3G257900_v2.0.a1 Prupe.5G003600_v2.0.a1 Prupe.5G053800_v2.0.a1 Prupe.6G011300_v2.0.a1 Prupe.7G161400_v2.0.a1 Prupe.7G161500_v2.0.a1 Prupe.7G188000_v2.0.a1 Prupe.8G182700_v2.0.a1 Prupe.8G182700_v2.0.a1 Prupe.8G182700_v2.0.a1
pyrus_communis pycom02g11030 pycom05g13970 pycom06g00250 pycom10g12390 pycom10g12400 pycom15g20220 pycom15g22260 pycom17g05620
rosa_chinensis RchiOBHm_Chr2g0096701 RchiOBHm_Chr2g0100471 RchiOBHm_Chr2g0100481 RchiOBHm_Chr6g0281561 RchiOBHm_Chr7g0187081 RchiOBHm_Chr7g0217201 RchiOBHm_Chr7g0228321
rosa_laevigata RLG00000001574 RLG00000002527 RLG00000008169 RLG00000011104 RLG00000012963 RLG00000013720 RLG00000014615 RLG00000016663 RLG00000018214 RLG00000018621 RLG00000018622 RLG00000022494
rosa_multiflora Rmu_co8187762.1_g000001 Rmu_co8276569.1_g000001 Rmu_sc0000528.1_g000025 Rmu_sc0001095.1_g000001 Rmu_sc0001095.1_g000002 Rmu_sc0001167.1_g000007 Rmu_sc0001758.1_g000042 Rmu_sc0002147.1_g000004 Rmu_sc0002489.1_g000048 Rmu_sc0002564.1_g000015 Rmu_sc0002902.1_g000037 Rmu_sc0003565.1_g000022 Rmu_sc0003576.1_g000008 Rmu_sc0003720.1_g000022 Rmu_sc0003720.1_g000023 Rmu_sc0003862.1_g000023 Rmu_sc0006475.1_g000009 Rmu_sc0006475.1_g000010 Rmu_sc0006735.1_g000001 Rmu_sc0007586.1_g000004 Rmu_sc0009650.1_g000010 Rmu_sc0010963.1_g000005 Rmu_sc0012557.1_g000002 Rmu_sc0020744.1_g000001 Rmu_sc0020745.1_g000002 Rmu_sc0022201.1_g000001 Rmu_sc0022408.1_g000011 Rmu_sc0023414.1_g000001 Rmu_sc0025101.1_g000001 Rmu_ssc0000211.1_g000015 Rmu_ssc0000240.1_g000012
rosa_roxburghii Rroxscaffold_1G00013940 Rroxscaffold_1G00018420 Rroxscaffold_2G00142210 Rroxscaffold_2G00142230 Rroxscaffold_2G00145400 Rroxscaffold_3G00240120 Rroxscaffold_3G00240150 Rroxscaffold_3G00242500 Rroxscaffold_5G00346610 Rroxscaffold_5G00356000 Rroxscaffold_7G00187000 Rroxscaffold_7G00212120
rosa_rugosa Rorug01G0260900 Rorug01G0446900 Rorug02G0059800 Rorug02G0059900 Rorug02G0088400 Rorug02G0088500 Rorug02G0522300 Rorug03G0234100 Rorug03G0234100 Rorug03G0242400 Rorug04G0109900 Rorug04G0326100 Rorug05G0275900 Rorug06G0141600 Rorug06G0141700 Rorug06G0141700 Rorug07G0167200 Rorug07G0247000 Rorug07G0247000 Rorug07G0247100.1
rosa_samantha Rh1AG046800 Rh1AG111600 Rh1AG242600 Rh1CG226400 Rh1DG086400 Rh2AG107200 Rh2AG138100 Rh2AG528600 Rh2BG109600 Rh2BG141200 Rh2BG273400 Rh2BG357800 Rh2BG640200 Rh2CG111400 Rh2CG143400 Rh2CG161100 Rh2CG251300 Rh2DG111000 Rh2DG142900 Rh2DG651100 Rh3BG318000 Rh4AG167800 Rh4AG253700 Rh5AG282900 Rh6AG254100 Rh6BG257100 Rh6CG256400 Rh6DG248200 Rh7AG306100 Rh7BG297500 Rh7CG325200 Rh7CG420200 Rh7DG306000
rosa_wichuraiana Rw2G008310 Rw2G010790 Rw2G010800 Rw2G013840 Rw2G022430 Rw3G020550 Rw3G024980 Rw3G025930 Rw5G026810 Rw5G035870 Rw6G022060 Rw6G030700 Rw7G007420 Rw7G026010 Rw7G033340 Rw7G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2013
Acc16I TGCGCA 1 cut(s) 973
Acc36I ACCTGC 1 cut(s) 748
AccB1I GGYRCC 1 cut(s) 1339
AccB7I CCANNNNNTGG 1 cut(s) 43
AccI GTMKAC 1 cut(s) 300
AccII CGCG 4 cut(s) 291, 318, 372, 2037
AccIII TCCGGA 1 cut(s) 514
AclI AACGTT 2 cut(s) 654, 1858
AclWI GGATC 4 cut(s) 761, 774, 1672, 2145
AcsI RAATTY 7 cut(s) 47, 221, 838, 1247, 1604, 1691, 2178
AcuI CTGAAG 4 cut(s) 441, 1235, 1292, 2105
AcyI GRCGYC 2 cut(s) 66, 1547
AdeI CACNNNGTG 1 cut(s) 1711
AfaI GTAC 2 cut(s) 406, 2131
AfeI AGCGCT 1 cut(s) 308
AfiI CCNNNNNNNGG 8 cut(s) 43, 181, 212, 406, 589, 932, 1495, 1680
AgeI ACCGGT 1 cut(s) 1125
AjnI CCWGG 3 cut(s) 789, 1071, 1353
Alw21I GWGCWC 2 cut(s) 627, 1716
Alw26I GTCTC 7 cut(s) 165, 536, 556, 861, 1060, 1229, 1619
Alw44I GTGCAC 1 cut(s) 1712
AlwI GGATC 4 cut(s) 761, 774, 1672, 2145
AlwNI CAGNNNCTG 1 cut(s) 1453
Aor13HI TCCGGA 1 cut(s) 514
Aor51HI AGCGCT 1 cut(s) 308
AoxI GGCC 1 cut(s) 237
ApaLI GTGCAC 1 cut(s) 1712
ApoI RAATTY 7 cut(s) 47, 221, 838, 1247, 1604, 1691, 2178
AseI ATTAAT 1 cut(s) 648
AsiGI ACCGGT 1 cut(s) 1125
Asp700I GAANNNNTTC 2 cut(s) 363, 1145
AspA2I CCTAGG 1 cut(s) 155
AspLEI GCGC 3 cut(s) 309, 399, 974
AspS9I GGNCC 4 cut(s) 190, 390, 557, 602
AsuC2I CCSGG 1 cut(s) 1113
AsuHPI GGTGA 3 cut(s) 392, 806, 1382
AsuNHI GCTAGC 1 cut(s) 1027
AvaII GGWCC 4 cut(s) 190, 390, 557, 602
AvrII CCTAGG 1 cut(s) 155
BaeGI GKGCMC 1 cut(s) 1716
BamHI GGATCC 1 cut(s) 766
BanI GGYRCC 1 cut(s) 1339
BauI CACGAG 1 cut(s) 162
BbsI GAAGAC 2 cut(s) 1707, 1801
Bbv12I GWGCWC 2 cut(s) 627, 1716
BccI CCATC 9 cut(s) 179, 314, 335, 459, 930, 1056, 1412, 1558, 1846
BceAI ACGGC 1 cut(s) 770
BcgI CGANNNNNNTGC 4 cut(s) 205, 239, 1613, 1647
BciT130I CCWGG 3 cut(s) 791, 1073, 1355
BciVI GTATCC 1 cut(s) 1351
BclI TGATCA 2 cut(s) 103, 127
BcnI CCSGG 1 cut(s) 1113
BcoDI GTCTC 7 cut(s) 165, 536, 556, 861, 1060, 1229, 1619
BfaI CTAG 4 cut(s) 156, 414, 606, 1028
BfoI RGCGCY 2 cut(s) 310, 400
BfuAI ACCTGC 1 cut(s) 748
BfuI GTATCC 1 cut(s) 1351
BlnI CCTAGG 1 cut(s) 155
Bme1390I CCNGG 4 cut(s) 791, 1073, 1113, 1355
Bme18I GGWCC 4 cut(s) 190, 390, 557, 602
BmgT120I GGNCC 4 cut(s) 190, 390, 557, 602
BmiI GGNNCC 6 cut(s) 191, 192, 325, 603, 768, 1341
BmrFI CCNGG 4 cut(s) 791, 1073, 1113, 1355
BmrI ACTGGG 2 cut(s) 189, 2093
BmsI GCATC 6 cut(s) 823, 959, 1230, 1312, 1442, 2017
BmtI GCTAGC 1 cut(s) 1031
BmuI ACTGGG 2 cut(s) 189, 2093
BoxI GACNNNNGTC 1 cut(s) 195
BpiI GAAGAC 2 cut(s) 1707, 1801
BpmI CTGGAG 1 cut(s) 1337
Bpu10I CCTNAGC 1 cut(s) 1158
BpuEI CTTGAG 1 cut(s) 1911
BpuMI CCSGG 1 cut(s) 1113
BsaHI GRCGYC 2 cut(s) 66, 1547
BsaI GGTCTC 1 cut(s) 165
BsaJI CCNNGG 5 cut(s) 155, 790, 1111, 1387, 2133
BsaWI WCCGGW 2 cut(s) 514, 1125
Bsc4I CCNNNNNNNGG 8 cut(s) 43, 181, 212, 406, 589, 932, 1495, 1680
Bse118I RCCGGY 1 cut(s) 1125
Bse1I ACTGG 5 cut(s) 195, 937, 1343, 1935, 2099
Bse3DI GCAATG 3 cut(s) 1041, 2028, 2198
BseAI TCCGGA 1 cut(s) 514
BseBI CCWGG 3 cut(s) 791, 1073, 1355
BseDI CCNNGG 5 cut(s) 155, 790, 1111, 1387, 2133
BseGI GGATG 7 cut(s) 33, 190, 941, 974, 1048, 1900, 2032
BseLI CCNNNNNNNGG 8 cut(s) 43, 181, 212, 406, 589, 932, 1495, 1680
BseMI GCAATG 3 cut(s) 1041, 2028, 2198
BseMII CTCAG 3 cut(s) 1172, 1224, 1532
BseNI ACTGG 5 cut(s) 195, 937, 1343, 1935, 2099
BseSI GKGCMC 1 cut(s) 1716
Bsh1236I CGCG 4 cut(s) 291, 318, 372, 2037
BshFI GGCC 1 cut(s) 239
BshNI GGYRCC 1 cut(s) 1339
BshTI ACCGGT 1 cut(s) 1125
BsiHKAI GWGCWC 2 cut(s) 627, 1716
BsiSI CCGG 3 cut(s) 515, 1113, 1126
BsiWI CGTACG 1 cut(s) 404
BslFI GGGAC 3 cut(s) 203, 899, 1252
BslI CCNNNNNNNGG 8 cut(s) 43, 181, 212, 406, 589, 932, 1495, 1680
BsmAI GTCTC 7 cut(s) 165, 536, 556, 861, 1060, 1229, 1619
BsmBI CGTCTC 1 cut(s) 1060
BsmFI GGGAC 3 cut(s) 203, 899, 1252
BsmI GAATGC 2 cut(s) 759, 2201
BsnI GGCC 1 cut(s) 239
Bso31I GGTCTC 1 cut(s) 165
Bsp1286I GDGCHC 2 cut(s) 627, 1716
Bsp13I TCCGGA 1 cut(s) 514
Bsp143I GATC 6 cut(s) 103, 127, 766, 1285, 1664, 2137
Bsp19I CCATGG 1 cut(s) 2133
Bsp68I TCGCGA 2 cut(s) 318, 2037
BspANI GGCC 1 cut(s) 239
BspCNI CTCAG 3 cut(s) 1171, 1225, 1533
BspEI TCCGGA 1 cut(s) 514
BspFNI CGCG 4 cut(s) 291, 318, 372, 2037
BspHI TCATGA 1 cut(s) 1599
BspLI GGNNCC 6 cut(s) 191, 192, 325, 603, 768, 1341
BspMI ACCTGC 1 cut(s) 748
BspOI GCTAGC 1 cut(s) 1031
BspPI GGATC 4 cut(s) 761, 774, 1672, 2145
BspQI GCTCTTC 2 cut(s) 1019, 2037
BspT107I GGYRCC 1 cut(s) 1339
BspTNI GGTCTC 1 cut(s) 165
BsrDI GCAATG 3 cut(s) 1041, 2028, 2198
BsrFI RCCGGY 1 cut(s) 1125
BsrI ACTGG 5 cut(s) 195, 937, 1343, 1935, 2099
BssAI RCCGGY 1 cut(s) 1125
BssECI CCNNGG 5 cut(s) 155, 790, 1111, 1387, 2133
BssMI GATC 6 cut(s) 103, 127, 766, 1285, 1664, 2137
BssNI GRCGYC 2 cut(s) 66, 1547
BssSI CACGAG 1 cut(s) 162
BssT1I CCWWGG 3 cut(s) 155, 1387, 2133
Bst2BI CACGAG 1 cut(s) 162
Bst2UI CCWGG 3 cut(s) 791, 1073, 1355
Bst4CI ACNGT 6 cut(s) 377, 409, 442, 874, 911, 1648
Bst6I CTCTTC 3 cut(s) 1019, 2037, 2151
BstACI GRCGYC 2 cut(s) 66, 1547
BstAPI GCANNNNNTGC 1 cut(s) 2027
BstC8I GCNNGC 4 cut(s) 401, 595, 701, 1029
BstDEI CTNAG 4 cut(s) 9, 1158, 1233, 1541
BstDSI CCRYGG 1 cut(s) 2133
BstF5I GGATG 7 cut(s) 33, 190, 941, 974, 1048, 1900, 2032
BstFNI CGCG 4 cut(s) 291, 318, 372, 2037
BstH2I RGCGCY 2 cut(s) 310, 400
BstHHI GCGC 3 cut(s) 309, 399, 974
BstKTI GATC 6 cut(s) 106, 130, 769, 1288, 1667, 2140
BstMAI GTCTC 7 cut(s) 165, 536, 556, 861, 1060, 1229, 1619
BstMBI GATC 6 cut(s) 103, 127, 766, 1285, 1664, 2137
BstMWI GCNNNNNNNGC 6 cut(s) 74, 315, 369, 1459, 1640, 2027
BstNI CCWGG 3 cut(s) 791, 1073, 1355
BstNSI RCATGY 3 cut(s) 551, 1106, 2009
BstPAI GACNNNNGTC 1 cut(s) 195
BstSCI CCNGG 4 cut(s) 789, 1071, 1111, 1353
BstSLI GKGCMC 1 cut(s) 1716
BstUI CGCG 4 cut(s) 291, 318, 372, 2037
BstV2I GAAGAC 2 cut(s) 1707, 1801
BstX2I RGATCY 1 cut(s) 766
BstYI RGATCY 1 cut(s) 766
BsuI GTATCC 1 cut(s) 1351
BsuRI GGCC 1 cut(s) 239
BtgI CCRYGG 1 cut(s) 2133
BtgZI GCGATG 1 cut(s) 333
BtsCI GGATG 7 cut(s) 33, 190, 941, 974, 1048, 1900, 2032
BtsIMutI CAGTG 3 cut(s) 99, 870, 1942
BtuMI TCGCGA 2 cut(s) 318, 2037
BveI ACCTGC 1 cut(s) 748
Cac8I GCNNGC 4 cut(s) 401, 595, 701, 1029
CaiI CAGNNNCTG 1 cut(s) 1453
CciI TCATGA 1 cut(s) 1599
CfoI GCGC 3 cut(s) 309, 399, 974
Cfr10I RCCGGY 1 cut(s) 1125
Cfr13I GGNCC 4 cut(s) 190, 390, 557, 602
CseI GACGC 4 cut(s) 74, 280, 286, 1555
Csp6I GTAC 2 cut(s) 405, 2130
CspAI ACCGGT 1 cut(s) 1125
CspCI CAANNNNNGTGG 2 cut(s) 1112, 1147
CviQI GTAC 2 cut(s) 405, 2130
DdeI CTNAG 4 cut(s) 9, 1158, 1233, 1541
DpnI GATC 6 cut(s) 105, 129, 768, 1287, 1666, 2139
DpnII GATC 6 cut(s) 103, 127, 766, 1285, 1664, 2137
DraIII CACNNNGTG 1 cut(s) 1711
Eam1104I CTCTTC 3 cut(s) 1019, 2037, 2151
EarI CTCTTC 3 cut(s) 1019, 2037, 2151
Eco130I CCWWGG 3 cut(s) 155, 1387, 2133
Eco147I AGGCCT 1 cut(s) 239
Eco31I GGTCTC 1 cut(s) 165
Eco32I GATATC 1 cut(s) 2259
Eco47I GGWCC 4 cut(s) 190, 390, 557, 602
Eco47III AGCGCT 1 cut(s) 308
Eco57I CTGAAG 4 cut(s) 441, 1235, 1292, 2105
EcoO109I RGGNCCY 2 cut(s) 190, 602
EcoRI GAATTC 1 cut(s) 221
EcoRII CCWGG 3 cut(s) 789, 1071, 1353
EcoRV GATATC 1 cut(s) 2259
EcoT14I CCWWGG 3 cut(s) 155, 1387, 2133
ErhI CCWWGG 3 cut(s) 155, 1387, 2133
Esp3I CGTCTC 1 cut(s) 1060
FaqI GGGAC 3 cut(s) 203, 899, 1252
FauI CCCGC 2 cut(s) 251, 296
FauNDI CATATG 1 cut(s) 332
FbaI TGATCA 2 cut(s) 103, 127
FblI GTMKAC 1 cut(s) 300
FokI GGATG 7 cut(s) 20, 197, 948, 981, 1035, 1907, 2039
FspAI RTGCGCAY 1 cut(s) 973
FspBI CTAG 4 cut(s) 156, 414, 606, 1028
FspI TGCGCA 1 cut(s) 973
GlaI GCGC 3 cut(s) 308, 398, 973
GsuI CTGGAG 1 cut(s) 1337
HaeII RGCGCY 2 cut(s) 310, 400
HaeIII GGCC 1 cut(s) 239
HapII CCGG 3 cut(s) 515, 1113, 1126
HgaI GACGC 4 cut(s) 74, 280, 286, 1555
HhaI GCGC 3 cut(s) 309, 399, 974
Hin1I GRCGYC 2 cut(s) 66, 1547
Hin6I GCGC 3 cut(s) 307, 397, 972
HinP1I GCGC 3 cut(s) 307, 397, 972
HpaII CCGG 3 cut(s) 515, 1113, 1126
HphI GGTGA 3 cut(s) 392, 806, 1382
Hpy166II GTNNAC 2 cut(s) 301, 1714
Hpy8I GTNNAC 2 cut(s) 301, 1714
Hpy99I CGWCG 3 cut(s) 273, 318, 377
HpyAV CCTTC 3 cut(s) 353, 593, 1982
HpyCH4III ACNGT 6 cut(s) 377, 409, 442, 874, 911, 1648
HpyCH4IV ACGT 3 cut(s) 654, 1143, 1858
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 315, 369, 1459, 1640, 2027
HpyF3I CTNAG 4 cut(s) 9, 1158, 1233, 1541
HpySE526I ACGT 3 cut(s) 654, 1143, 1858
Hsp92I GRCGYC 2 cut(s) 66, 1547
HspAI GCGC 3 cut(s) 307, 397, 972
KflI GGGWCCC 1 cut(s) 190
Kpn2I TCCGGA 1 cut(s) 514
Ksp22I TGATCA 2 cut(s) 103, 127
Kzo9I GATC 6 cut(s) 103, 127, 766, 1285, 1664, 2137
LguI GCTCTTC 2 cut(s) 1019, 2037
LmnI GCTCC 3 cut(s) 314, 329, 356
LweI GCATC 6 cut(s) 823, 959, 1230, 1312, 1442, 2017
MaeI CTAG 4 cut(s) 156, 414, 606, 1028
MaeII ACGT 3 cut(s) 654, 1143, 1858
MaeIII GTNAC 4 cut(s) 409, 874, 1228, 1816
MalI GATC 6 cut(s) 105, 129, 768, 1287, 1666, 2139
MboI GATC 6 cut(s) 103, 127, 766, 1285, 1664, 2137
MfeI CAATTG 4 cut(s) 1239, 1476, 1763, 2082
MflI RGATCY 1 cut(s) 766
MhlI GDGCHC 2 cut(s) 627, 1716
MlyI GAGTC 7 cut(s) 20, 221, 644, 998, 1004, 1262, 1274
MmeI TCCRAC 3 cut(s) 1197, 1841, 2154
MroI TCCGGA 1 cut(s) 514
MroXI GAANNNNTTC 2 cut(s) 363, 1145
MseI TTAA 5 cut(s) 648, 984, 1722, 1853, 2252
MslI CAYNNNNRTG 6 cut(s) 38, 111, 498, 546, 1226, 1448
MspA1I CMGCKG 2 cut(s) 1758, 2168
MspI CCGG 3 cut(s) 515, 1113, 1126
MspR9I CCNGG 4 cut(s) 791, 1073, 1113, 1355
MunI CAATTG 4 cut(s) 1239, 1476, 1763, 2082
Mva1269I GAATGC 2 cut(s) 759, 2201
MvaI CCWGG 3 cut(s) 791, 1073, 1355
MvnI CGCG 4 cut(s) 291, 318, 372, 2037
MwoI GCNNNNNNNGC 6 cut(s) 74, 315, 369, 1459, 1640, 2027
NciI CCSGG 1 cut(s) 1113
NcoI CCATGG 1 cut(s) 2133
NdeI CATATG 1 cut(s) 332
NdeII GATC 6 cut(s) 103, 127, 766, 1285, 1664, 2137
NheI GCTAGC 1 cut(s) 1027
NlaIV GGNNCC 6 cut(s) 191, 192, 325, 603, 768, 1341
NmeAIII GCCGAG 1 cut(s) 1306
NmuCI GTSAC 1 cut(s) 1228
NruI TCGCGA 2 cut(s) 318, 2037
NsbI TGCGCA 1 cut(s) 973
NspI RCATGY 3 cut(s) 551, 1106, 2009
PagI TCATGA 1 cut(s) 1599
PceI AGGCCT 1 cut(s) 239
PciSI GCTCTTC 2 cut(s) 1019, 2037
PctI GAATGC 2 cut(s) 759, 2201
PdmI GAANNNNTTC 2 cut(s) 363, 1145
PfeI GAWTC 7 cut(s) 940, 1080, 1537, 1922, 1931, 1997, 2068
Pfl23II CGTACG 1 cut(s) 404
PflMI CCANNNNNTGG 1 cut(s) 43
PfoI TCCNGGA 1 cut(s) 1353
PinAI ACCGGT 1 cut(s) 1125
PleI GAGTC 7 cut(s) 20, 221, 643, 997, 1003, 1261, 1273
PpsI GAGTC 7 cut(s) 20, 221, 643, 997, 1003, 1261, 1273
PpuMI RGGWCCY 2 cut(s) 190, 602
PshAI GACNNNNGTC 1 cut(s) 195
PshBI ATTAAT 1 cut(s) 648
PsiI TTATAA 1 cut(s) 2013
Psp1406I AACGTT 2 cut(s) 654, 1858
Psp5II RGGWCCY 2 cut(s) 190, 602
Psp6I CCWGG 3 cut(s) 789, 1071, 1353
PspGI CCWGG 3 cut(s) 789, 1071, 1353
PspLI CGTACG 1 cut(s) 404
PspN4I GGNNCC 6 cut(s) 191, 192, 325, 603, 768, 1341
PspPI GGNCC 4 cut(s) 190, 390, 557, 602
PspPPI RGGWCCY 2 cut(s) 190, 602
PsrI GAACNNNNNNTAC 2 cut(s) 1809, 1841
PstNI CAGNNNCTG 1 cut(s) 1453
PsuI RGATCY 1 cut(s) 766
PvuII CAGCTG 2 cut(s) 1758, 2168
RruI TCGCGA 2 cut(s) 318, 2037
RsaI GTAC 2 cut(s) 406, 2131
RsaNI GTAC 2 cut(s) 405, 2130
RseI CAYNNNNRTG 6 cut(s) 38, 111, 498, 546, 1226, 1448
SapI GCTCTTC 2 cut(s) 1019, 2037
SaqAI TTAA 5 cut(s) 648, 984, 1722, 1853, 2252
Sau3AI GATC 6 cut(s) 103, 127, 766, 1285, 1664, 2137
Sau96I GGNCC 4 cut(s) 190, 390, 557, 602
SchI GAGTC 7 cut(s) 20, 221, 644, 998, 1004, 1262, 1274
ScrFI CCNGG 4 cut(s) 791, 1073, 1113, 1355
SduI GDGCHC 2 cut(s) 627, 1716
SfaNI GCATC 6 cut(s) 823, 959, 1230, 1312, 1442, 2017
SinI GGWCC 4 cut(s) 190, 390, 557, 602
SmiMI CAYNNNNRTG 6 cut(s) 38, 111, 498, 546, 1226, 1448
SmlI CTYRAG 1 cut(s) 1926
SmoI CTYRAG 1 cut(s) 1926
SseBI AGGCCT 1 cut(s) 239
SspI AATATT 1 cut(s) 1596
SspMI CTAG 4 cut(s) 156, 414, 606, 1028
StuI AGGCCT 1 cut(s) 239
StyD4I CCNGG 4 cut(s) 789, 1071, 1111, 1353
StyI CCWWGG 3 cut(s) 155, 1387, 2133
TaaI ACNGT 6 cut(s) 377, 409, 442, 874, 911, 1648
TaiI ACGT 3 cut(s) 657, 1146, 1861
TaqI TCGA 9 cut(s) 123, 225, 271, 565, 762, 809, 842, 1040, 1623
TauI GCSGC 6 cut(s) 71, 339, 372, 593, 1047, 2060
TfiI GAWTC 7 cut(s) 940, 1080, 1537, 1922, 1931, 1997, 2068
Tru1I TTAA 5 cut(s) 648, 984, 1722, 1853, 2252
Tru9I TTAA 5 cut(s) 648, 984, 1722, 1853, 2252
TscAI CASTG 3 cut(s) 106, 877, 1942
TseFI GTSAC 1 cut(s) 1228
Tsp45I GTSAC 1 cut(s) 1228
TspDTI ATGAA 6 cut(s) 863, 1482, 1506, 1538, 1588, 1616
TspGWI ACGGA 2 cut(s) 524, 963
TspRI CASTG 3 cut(s) 106, 877, 1942
Van91I CCANNNNNTGG 1 cut(s) 43
VneI GTGCAC 1 cut(s) 1712
VpaK11BI GGWCC 4 cut(s) 190, 390, 557, 602
VspI ATTAAT 1 cut(s) 648
XapI RAATTY 7 cut(s) 47, 221, 838, 1247, 1604, 1691, 2178
XceI RCATGY 3 cut(s) 551, 1106, 2009
XcmI CCANNNNNNNNNTGG 1 cut(s) 1681
XmaJI CCTAGG 1 cut(s) 155
XmiI GTMKAC 1 cut(s) 300
XmnI GAANNNNTTC 2 cut(s) 363, 1145
XspI CTAG 4 cut(s) 156, 414, 606, 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.