pycom15g20220

Protein FAR1-RELATED SEQUENCE 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000165
Physical Location & Seq
Reverse (-)
14423141 .. 14425648
2508 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g20220.2

Sequence Viewer

Length: 2241 bp
ATGGAGTTTGAACCGTTGAGCTTGGGTAATGATGATGTTCTTGAATTCGATATGATGGGTCTGGGTTACATTGACCAACCTGTTGACCATGATGACCTCCTTCATGATTCTCCTGCCACTGCTATTCCCACTCCTACTGGTTCCGATGTTGTCAGTAGCGTTGCTATTTTGGAGCATTACATCCCGGAAGGAGACAGTAGCCTTGAACCCTGTGAGGGGATGGAATTCGAATCCGAGGAGGCTGCCAAGGCCTTTTACAATTCCTATGCTCGCCGCATTGGCTTCAGTACCCGTGTCAGCATGTCTCGTCGCTCCAGGCGCGATGGCGCCATCATTCAGCGGTCTTTTGTTTGCGCCAAGGAGGGATTTCGTGTCAACAAGAATGGGAAGTCTGGGTCTGACTGCGAAGGGCGGGCCAAGCGCCCCCGAGCAGAGACCCGAGTTGGTTGTAAGGCCATGTTGGTAATAAAGGTTCACGACTCTGGGAGATGGGTTGTGTCTGCTTTTGTTAAGGAGCATAACCATGAGCTCGTTCCACCTGATAAGGTGCATTCCCTTCGCTCTCATCGTCATGTCTCGGGTTCTGCAAAGTCTTTGATTGATACCTTGCAGGGTGCTGGGATCGGCCCCAGAGGAATTATGTCTGCCCTCATCAAAGAATATGGTGGTATTAGGAATGTTGGCTTCACGGAGCGTGACTGCAGGAATTACATGAGGAGTTGTAAGCAAAGGACCCTTGGAGGCGACACTCAGCTGCTCTTGGATTACTTGAAGAGCAGGCAAGCTAAGGACCCAGCATTCTTCTTTGCTGTGCAGGGAGATGAAGATCAGTGCATGGGCAATTTCTTCTGGGTTGATCCTAAGGCAAGGAATGACTATACTTACTTTGGTGACACTGTTACCTTTGACACAACTTACAGGTCAAACCGCTATCGGTTGCCTTTTGCACCTTTCACAGGGGTTAACCATCATGGACAGCCTGTATTTTTTGGATGTGCCCTCCTGATAAATGAATCTGAGGCATCGTTTCTGTGGCTTTTCAAAACATGGCTCAAGGCAATGTCTGGCCAGCCTCCACTGTCAATTACGACTGATCATGATCGGGTGATTACGATGGCCATTACCCAGGTTTTCCCCGAGACTCGACACCGCTTCTGCAAGTGGCACATATTTAAAGAATGCCAAGAGAAGTTGTCCTCTGTGCTTTGTGAGCATTCCAACTTTGAAGCAGAGCTTCATAAATGTGTCAACCTGAGTGAATCAATTGAAGAGTTTGAGTCTTGTTGGCTGTCCCTAACTGGTAAATATGATCTCGGGGAGCATAAATGGCTTCAATCGATGTATGCTGATCGGCAGCAGTGGGTCCCTGTGTACCTGAGGGGCACCTTCTTTGCAGAAATGTCGGTAACACAGTGCAGTGATAGCATGAACTCTTATTTTGATGGATATGTCAATGCATCAACAACTCTACAGCTGTTTGTTAAGCAGTATGAGAAAGCTGTTGAAAGTCGGTATGAGAAAGAAGTGAGGGCAGATTATGATACCATAAACACTGCTCCGGTTCTTAAGACCCCTTCGCCCATGGAGAAACAGGCAGCTGGGCTTTACACGAGGAAATTGTTTACGAAATTTCAAGAGGAGTTGGTTGAGACGTTAACATTTTTGGCAACCAAGGTTGAGGACGAGGAGGCAGGATCCTTTTTTCAAGTTGCTAAATTTGGGGAGAGTCATAAAGCTTATACGCGTTGGACGAGGAATGCCAAGAGTGGTGTCGTGTTGGAGGAACATGCCGGCGATTTGTTGAATAAGCCACAAGAATCTCTAACAGTACGGTACAATAATCTGCGGCACGAGGCACTTAAATATGTTGACGAAGGGATTACAACTTGCGAAATTTATAATGTGGCAATGGATGCCCTTCAAGTGGCTGCAAATAAAGTTGCCACTGAAATGCAGAACGGCAGGAGAGCGGCCAGAACTAATACAGCTGGAAGAGAAGATGGGTTTCGTTTGGGAAATCAATCAAACAATACCTTCGGTGATCATAAACGGGGATTTGAAACTCTATCCGCGGATCACCAGGACAAGAAAATGCAGAAACTAGAGCGTCAACTCGAGCGCGCACGACGGAAATGTGAATCGTATAGAGCTAACTTGCTATCGGTTTTGAAAGATATTGAGGAGCAAAAGTTAGAGCTCTGTGTTAAAGTTGAAAACATTAAGTTGAGAATGAAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1 MULE SWIM

Protein Analysis

747

Amino Acids

85.01

Weight (kDa)

6.46

Isoelectric Point (pI)

47.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38180
fragaria_vesca FvH4_1g09751 FvH4_1g09751 FvH4_1g09751 FvH4_1g12531 FvH4_1g19193 FvH4_2g07051 FvH4_2g17131 FvH4_2g17131 FvH4_2g28082 FvH4_5g08845 FvH4_5g26361 FvH4_5g26361 FvH4_5g26361 FvH4_5g26361 FvH4_5g39269 FvH4_6g36651 FvH4_6g38410 FvH4_7g04941 FvH4_7g04941
malus_domestica MD00G1156400.v1.1 MD02G1139600.v1.1 MD05G1144600.v1.1 MD06G1002400.v1.1 MD10G1143900.v1.1 MD15G1227300.v1.1 MD15G1252700.v1.1
prunus_persica Prupe.2G147900_v2.0.a1 Prupe.3G257900_v2.0.a1 Prupe.5G003600_v2.0.a1 Prupe.5G053800_v2.0.a1 Prupe.6G011300_v2.0.a1 Prupe.7G161400_v2.0.a1 Prupe.7G161500_v2.0.a1 Prupe.7G188000_v2.0.a1 Prupe.8G182700_v2.0.a1 Prupe.8G182700_v2.0.a1 Prupe.8G182700_v2.0.a1
pyrus_communis pycom02g11030 pycom05g13970 pycom06g00250 pycom10g12390 pycom10g12400 pycom15g20220 pycom15g22260 pycom17g05620
rosa_chinensis RchiOBHm_Chr2g0096701 RchiOBHm_Chr2g0100471 RchiOBHm_Chr2g0100481 RchiOBHm_Chr6g0281561 RchiOBHm_Chr7g0187081 RchiOBHm_Chr7g0217201 RchiOBHm_Chr7g0228321
rosa_laevigata RLG00000001574 RLG00000002527 RLG00000008169 RLG00000011104 RLG00000012963 RLG00000013720 RLG00000014615 RLG00000016663 RLG00000018214 RLG00000018621 RLG00000018622 RLG00000022494
rosa_multiflora Rmu_co8187762.1_g000001 Rmu_co8276569.1_g000001 Rmu_sc0000528.1_g000025 Rmu_sc0001095.1_g000001 Rmu_sc0001095.1_g000002 Rmu_sc0001167.1_g000007 Rmu_sc0001758.1_g000042 Rmu_sc0002147.1_g000004 Rmu_sc0002489.1_g000048 Rmu_sc0002564.1_g000015 Rmu_sc0002902.1_g000037 Rmu_sc0003565.1_g000022 Rmu_sc0003576.1_g000008 Rmu_sc0003720.1_g000022 Rmu_sc0003720.1_g000023 Rmu_sc0003862.1_g000023 Rmu_sc0006475.1_g000009 Rmu_sc0006475.1_g000010 Rmu_sc0006735.1_g000001 Rmu_sc0007586.1_g000004 Rmu_sc0009650.1_g000010 Rmu_sc0010963.1_g000005 Rmu_sc0012557.1_g000002 Rmu_sc0020744.1_g000001 Rmu_sc0020745.1_g000002 Rmu_sc0022201.1_g000001 Rmu_sc0022408.1_g000011 Rmu_sc0023414.1_g000001 Rmu_sc0025101.1_g000001 Rmu_ssc0000211.1_g000015 Rmu_ssc0000240.1_g000012
rosa_roxburghii Rroxscaffold_1G00013940 Rroxscaffold_1G00018420 Rroxscaffold_2G00142210 Rroxscaffold_2G00142230 Rroxscaffold_2G00145400 Rroxscaffold_3G00240120 Rroxscaffold_3G00240150 Rroxscaffold_3G00242500 Rroxscaffold_5G00346610 Rroxscaffold_5G00356000 Rroxscaffold_7G00187000 Rroxscaffold_7G00212120
rosa_rugosa Rorug01G0260900 Rorug01G0446900 Rorug02G0059800 Rorug02G0059900 Rorug02G0088400 Rorug02G0088500 Rorug02G0522300 Rorug03G0234100 Rorug03G0234100 Rorug03G0242400 Rorug04G0109900 Rorug04G0326100 Rorug05G0275900 Rorug06G0141600 Rorug06G0141700 Rorug06G0141700 Rorug07G0167200 Rorug07G0247000 Rorug07G0247000 Rorug07G0247100.1
rosa_samantha Rh1AG046800 Rh1AG111600 Rh1AG242600 Rh1CG226400 Rh1DG086400 Rh2AG107200 Rh2AG138100 Rh2AG528600 Rh2BG109600 Rh2BG141200 Rh2BG273400 Rh2BG357800 Rh2BG640200 Rh2CG111400 Rh2CG143400 Rh2CG161100 Rh2CG251300 Rh2DG111000 Rh2DG142900 Rh2DG651100 Rh3BG318000 Rh4AG167800 Rh4AG253700 Rh5AG282900 Rh6AG254100 Rh6BG257100 Rh6CG256400 Rh6DG248200 Rh7AG306100 Rh7BG297500 Rh7CG325200 Rh7CG420200 Rh7DG306000
rosa_wichuraiana Rw2G008310 Rw2G010790 Rw2G010800 Rw2G013840 Rw2G022430 Rw3G020550 Rw3G024980 Rw3G025930 Rw5G026810 Rw5G035870 Rw6G022060 Rw6G030700 Rw7G007420 Rw7G026010 Rw7G033340 Rw7G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1899
AccB1I GGYRCC 2 cut(s) 326, 1382
AccBSI CCGCTC 1 cut(s) 1970
AccII CGCG 4 cut(s) 321, 1744, 2072, 2121
AciI CCGC 9 cut(s) 274, 340, 412, 928, 1150, 1846, 1970, 2070, 2072
AclWI GGATC 5 cut(s) 629, 851, 1689, 1702, 2082
AcoI YGGCCR 3 cut(s) 1066, 1116, 1971
AcsI RAATTY 5 cut(s) 44, 224, 1628, 1715, 1893
AcuI CTGAAG 1 cut(s) 268
AcyI GRCGYC 1 cut(s) 327
AfaI GTAC 4 cut(s) 289, 1373, 1830, 1835
AfiI CCNNNNNNNGG 4 cut(s) 215, 216, 956, 1924
AflII CTTAAG 1 cut(s) 1565
AflIII ACRYGT 1 cut(s) 1742
AjnI CCWGG 3 cut(s) 314, 1125, 2079
Alw21I GWGCWC 2 cut(s) 531, 2199
Alw26I GTCTC 6 cut(s) 186, 309, 428, 580, 1133, 1643
AlwI GGATC 5 cut(s) 629, 851, 1689, 1702, 2082
Ama87I CYCGRG 6 cut(s) 426, 438, 577, 1136, 1313, 2114
AoxI GGCC 7 cut(s) 249, 414, 453, 625, 1066, 1116, 1971
ApeKI GCWGC 5 cut(s) 242, 754, 1354, 1595, 1928
ApoI RAATTY 5 cut(s) 44, 224, 1628, 1715, 1893
AspLEI GCGC 6 cut(s) 321, 329, 356, 423, 2121, 2123
AspS9I GGNCC 5 cut(s) 414, 626, 732, 790, 1363
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 4 cut(s) 902, 1117, 2051, 2069
AsuII TTCGAA 1 cut(s) 228
AvaI CYCGRG 6 cut(s) 426, 438, 577, 1136, 1313, 2114
AvaII GGWCC 3 cut(s) 732, 790, 1363
AxyI CCTNAGG 2 cut(s) 861, 1376
BaeGI GKGCMC 2 cut(s) 1000, 1385
BalI TGGCCA 2 cut(s) 1068, 1118
BamHI GGATCC 1 cut(s) 1694
BanI GGYRCC 2 cut(s) 326, 1382
BanII GRGCYC 2 cut(s) 531, 2199
BauI CACGAG 2 cut(s) 1609, 1850
Bbv12I GWGCWC 2 cut(s) 531, 2199
BbvI GCAGC 5 cut(s) 229, 741, 1366, 1607, 1915
BccI CCATC 9 cut(s) 49, 214, 317, 338, 483, 975, 1108, 1436, 1994
BceAI ACGGC 1 cut(s) 1975
BcgI CGANNNNNNTGC 6 cut(s) 224, 258, 539, 573, 1383, 1417
BciT130I CCWGG 3 cut(s) 316, 1127, 2081
BclI TGATCA 2 cut(s) 1093, 2041
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 6 cut(s) 186, 309, 428, 580, 1133, 1643
BfaI CTAG 1 cut(s) 2102
BfmI CTRYAG 2 cut(s) 700, 1469
BfoI RGCGCY 2 cut(s) 330, 424
BfrI CTTAAG 1 cut(s) 1565
BglI GCCNNNNNGGC 1 cut(s) 279
BisI GCNGC 8 cut(s) 243, 274, 755, 1355, 1596, 1847, 1929, 1971
BlsI GCNGC 8 cut(s) 244, 275, 756, 1356, 1597, 1848, 1930, 1972
Bme1390I CCNGG 4 cut(s) 185, 316, 1127, 2081
Bme18I GGWCC 3 cut(s) 732, 790, 1363
BmeT110I CYCGRG 6 cut(s) 426, 438, 577, 1136, 1313, 2114
BmgT120I GGNCC 5 cut(s) 414, 626, 732, 790, 1363
BmiI GGNNCC 9 cut(s) 142, 328, 628, 734, 792, 1364, 1365, 1384, 1696
BmrFI CCNGG 4 cut(s) 185, 316, 1127, 2081
BmsI GCATC 3 cut(s) 1031, 1466, 1903
BpmI CTGGAG 1 cut(s) 298
Bpu10I CCTNAGC 1 cut(s) 786
Bpu14I TTCGAA 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 1037
BpuMI CCSGG 1 cut(s) 185
Bsa29I ATCGAT 1 cut(s) 1337
BsaBI GATNNNNATC 2 cut(s) 825, 1098
BsaHI GRCGYC 1 cut(s) 327
BsaI GGTCTC 1 cut(s) 428
BsaJI CCNNGG 8 cut(s) 234, 246, 357, 736, 1125, 1581, 1671, 2070
BsaWI WCCGGW 1 cut(s) 1558
BsaXI ACNNNNNCTCC 2 cut(s) 2174, 2204
Bsc4I CCNNNNNNNGG 4 cut(s) 215, 216, 956, 1924
Bse118I RCCGGY 1 cut(s) 1790
Bse1I ACTGG 2 cut(s) 142, 1303
Bse21I CCTNAGG 2 cut(s) 861, 1376
Bse3DI GCAATG 2 cut(s) 1065, 1914
Bse8I GATNNNNATC 2 cut(s) 825, 1098
BseBI CCWGG 3 cut(s) 316, 1127, 2081
BseCI ATCGAT 1 cut(s) 1337
BseDI CCNNGG 8 cut(s) 234, 246, 357, 736, 1125, 1581, 1671, 2070
BseGI GGATG 4 cut(s) 180, 225, 998, 1918
BseJI GATNNNNATC 2 cut(s) 825, 1098
BseLI CCNNNNNNNGG 4 cut(s) 215, 216, 956, 1924
BseMI GCAATG 2 cut(s) 1065, 1914
BseMII CTCAG 4 cut(s) 764, 1008, 1244, 1367
BseNI ACTGG 2 cut(s) 142, 1303
BsePI GCGCGC 1 cut(s) 2119
BseRI GAGGAG 5 cut(s) 251, 730, 1652, 1700, 2195
BseSI GKGCMC 2 cut(s) 1000, 1385
BseXI GCAGC 5 cut(s) 229, 741, 1366, 1607, 1915
BseYI CCCAGC 3 cut(s) 617, 793, 1598
BsgI GTGCAG 2 cut(s) 833, 1435
Bsh1236I CGCG 4 cut(s) 321, 1744, 2072, 2121
BshFI GGCC 7 cut(s) 251, 416, 455, 627, 1068, 1118, 1973
BshNI GGYRCC 2 cut(s) 326, 1382
BshVI ATCGAT 1 cut(s) 1337
BsiHKAI GWGCWC 2 cut(s) 531, 2199
BsiHKCI CYCGRG 6 cut(s) 426, 438, 577, 1136, 1313, 2114
BsiSI CCGG 3 cut(s) 185, 1559, 1791
BslFI GGGAC 2 cut(s) 1276, 1349
BslI CCNNNNNNNGG 4 cut(s) 215, 216, 956, 1924
BsmAI GTCTC 6 cut(s) 186, 309, 428, 580, 1133, 1643
BsmBI CGTCTC 1 cut(s) 1643
BsmFI GGGAC 2 cut(s) 1276, 1349
BsmI GAATGC 5 cut(s) 550, 797, 1184, 1213, 1762
BsnI GGCC 7 cut(s) 251, 416, 455, 627, 1068, 1118, 1973
Bso31I GGTCTC 1 cut(s) 428
BsoBI CYCGRG 6 cut(s) 426, 438, 577, 1136, 1313, 2114
Bsp119I TTCGAA 1 cut(s) 228
Bsp1286I GDGCHC 4 cut(s) 531, 1000, 1385, 2199
Bsp19I CCATGG 1 cut(s) 1581
BspACI CCGC 9 cut(s) 274, 340, 412, 928, 1150, 1846, 1970, 2070, 2072
BspANI GGCC 7 cut(s) 251, 416, 455, 627, 1068, 1118, 1973
BspCNI CTCAG 4 cut(s) 763, 1009, 1245, 1368
BspDI ATCGAT 1 cut(s) 1337
BspFNI CGCG 4 cut(s) 321, 1744, 2072, 2121
BspHI TCATGA 2 cut(s) 103, 1096
BspLI GGNNCC 9 cut(s) 142, 328, 628, 734, 792, 1364, 1365, 1384, 1696
BspMAI CTGCAG 1 cut(s) 704
BspPI GGATC 5 cut(s) 629, 851, 1689, 1702, 2082
BspQI GCTCTTC 1 cut(s) 767
BspT104I TTCGAA 1 cut(s) 228
BspT107I GGYRCC 2 cut(s) 326, 1382
BspTI CTTAAG 1 cut(s) 1565
BspTNI GGTCTC 1 cut(s) 428
BsrBI CCGCTC 1 cut(s) 1970
BsrDI GCAATG 2 cut(s) 1065, 1914
BsrFI RCCGGY 1 cut(s) 1790
BsrI ACTGG 2 cut(s) 142, 1303
BssAI RCCGGY 1 cut(s) 1790
BssECI CCNNGG 8 cut(s) 234, 246, 357, 736, 1125, 1581, 1671, 2070
BssHII GCGCGC 1 cut(s) 2119
BssNI GRCGYC 1 cut(s) 327
BssSI CACGAG 2 cut(s) 1609, 1850
BssT1I CCWWGG 5 cut(s) 246, 357, 736, 1581, 1671
Bst2BI CACGAG 2 cut(s) 1609, 1850
Bst2UI CCWGG 3 cut(s) 316, 1127, 2081
Bst4CI ACNGT 7 cut(s) 15, 197, 898, 1080, 1413, 1828, 1833
Bst6I CTCTTC 3 cut(s) 767, 1263, 1987
BstACI GRCGYC 1 cut(s) 327
BstAFI CTTAAG 1 cut(s) 1565
BstAPI GCANNNNNTGC 1 cut(s) 1913
BstBI TTCGAA 1 cut(s) 228
BstC8I GCNNGC 7 cut(s) 271, 414, 779, 783, 1070, 1792, 2121
BstDEI CTNAG 6 cut(s) 750, 786, 861, 1017, 1253, 1376
BstDSI CCRYGG 2 cut(s) 1581, 2070
BstENI CCTNNNNNAGG 1 cut(s) 954
BstF5I GGATG 4 cut(s) 180, 225, 998, 1918
BstFNI CGCG 4 cut(s) 321, 1744, 2072, 2121
BstH2I RGCGCY 2 cut(s) 330, 424
BstHHI GCGC 6 cut(s) 321, 329, 356, 423, 2121, 2123
BstMAI GTCTC 6 cut(s) 186, 309, 428, 580, 1133, 1643
BstMWI GCNNNNNNNGC 8 cut(s) 248, 279, 318, 418, 1210, 1327, 1422, 1913
BstNI CCWGG 3 cut(s) 316, 1127, 2081
BstNSI RCATGY 2 cut(s) 304, 1790
BstSCI CCNGG 4 cut(s) 183, 314, 1125, 2079
BstSFI CTRYAG 2 cut(s) 700, 1469
BstSLI GKGCMC 2 cut(s) 1000, 1385
BstUI CGCG 4 cut(s) 321, 1744, 2072, 2121
BstV1I GCAGC 5 cut(s) 229, 741, 1366, 1607, 1915
BstX2I RGATCY 1 cut(s) 1694
BstYI RGATCY 1 cut(s) 1694
Bsu15I ATCGAT 1 cut(s) 1337
Bsu36I CCTNAGG 2 cut(s) 861, 1376
BsuRI GGCC 7 cut(s) 251, 416, 455, 627, 1068, 1118, 1973
BsuTUI ATCGAT 1 cut(s) 1337
BtgI CCRYGG 2 cut(s) 1581, 2070
BtgZI GCGATG 1 cut(s) 336
BtsCI GGATG 4 cut(s) 180, 225, 998, 1918
BtsI GCAGTG 4 cut(s) 117, 1364, 1423, 1551
BtsIMutI CAGTG 9 cut(s) 117, 836, 894, 1076, 1364, 1418, 1423, 1551, 1944
Cac8I GCNNGC 7 cut(s) 271, 414, 779, 783, 1070, 1792, 2121
CciI TCATGA 2 cut(s) 103, 1096
CfoI GCGC 6 cut(s) 321, 329, 356, 423, 2121, 2123
Cfr10I RCCGGY 1 cut(s) 1790
Cfr13I GGNCC 5 cut(s) 414, 626, 732, 790, 1363
Cfr42I CCGCGG 1 cut(s) 2073
ClaI ATCGAT 1 cut(s) 1337
CseI GACGC 1 cut(s) 2096
Csp6I GTAC 4 cut(s) 288, 1372, 1829, 1834
CviQI GTAC 4 cut(s) 288, 1372, 1829, 1834
DdeI CTNAG 6 cut(s) 750, 786, 861, 1017, 1253, 1376
DinI GGCGCC 1 cut(s) 328
DraI TTTAAA 1 cut(s) 1174
EaeI YGGCCR 3 cut(s) 1066, 1116, 1971
Eam1104I CTCTTC 3 cut(s) 767, 1263, 1987
EarI CTCTTC 3 cut(s) 767, 1263, 1987
Ecl136II GAGCTC 2 cut(s) 529, 2197
Eco130I CCWWGG 5 cut(s) 246, 357, 736, 1581, 1671
Eco147I AGGCCT 1 cut(s) 251
Eco24I GRGCYC 2 cut(s) 531, 2199
Eco31I GGTCTC 1 cut(s) 428
Eco47I GGWCC 3 cut(s) 732, 790, 1363
Eco53kI GAGCTC 2 cut(s) 529, 2197
Eco57I CTGAAG 1 cut(s) 268
Eco81I CCTNAGG 2 cut(s) 861, 1376
Eco88I CYCGRG 6 cut(s) 426, 438, 577, 1136, 1313, 2114
EcoICRI GAGCTC 2 cut(s) 529, 2197
EcoNI CCTNNNNNAGG 1 cut(s) 954
EcoO109I RGGNCCY 3 cut(s) 732, 790, 1363
EcoRI GAATTC 2 cut(s) 44, 224
EcoRII CCWGG 3 cut(s) 314, 1125, 2079
EcoT14I CCWWGG 5 cut(s) 246, 357, 736, 1581, 1671
EcoT22I ATGCAT 1 cut(s) 1459
EcoT38I GRGCYC 2 cut(s) 531, 2199
EgeI GGCGCC 1 cut(s) 328
EheI GGCGCC 1 cut(s) 328
ErhI CCWWGG 5 cut(s) 246, 357, 736, 1581, 1671
Esp3I CGTCTC 1 cut(s) 1643
FalI AAGNNNNNCTT 2 cut(s) 1218, 1250
FaqI GGGAC 2 cut(s) 1276, 1349
FauI CCCGC 1 cut(s) 405
FbaI TGATCA 2 cut(s) 1093, 2041
Fnu4HI GCNGC 8 cut(s) 243, 274, 755, 1355, 1596, 1847, 1929, 1971
FokI GGATG 4 cut(s) 167, 232, 1005, 1925
FriOI GRGCYC 2 cut(s) 531, 2199
Fsp4HI GCNGC 8 cut(s) 243, 274, 755, 1355, 1596, 1847, 1929, 1971
FspBI CTAG 1 cut(s) 2102
GlaI GCGC 6 cut(s) 320, 328, 355, 422, 2120, 2122
GluI GCNGC 8 cut(s) 243, 274, 755, 1355, 1596, 1847, 1929, 1971
GsaI CCCAGC 3 cut(s) 621, 797, 1602
GsuI CTGGAG 1 cut(s) 298
HaeII RGCGCY 2 cut(s) 330, 424
HaeIII GGCC 7 cut(s) 251, 416, 455, 627, 1068, 1118, 1973
HapII CCGG 3 cut(s) 185, 1559, 1791
HgaI GACGC 1 cut(s) 2096
HhaI GCGC 6 cut(s) 321, 329, 356, 423, 2121, 2123
Hin1I GRCGYC 1 cut(s) 327
Hin6I GCGC 6 cut(s) 319, 327, 354, 421, 2119, 2121
HinP1I GCGC 6 cut(s) 319, 327, 354, 421, 2119, 2121
HincII GTYRAC 7 cut(s) 85, 376, 964, 1249, 1656, 1870, 2111
HindII GTYRAC 7 cut(s) 85, 376, 964, 1249, 1656, 1870, 2111
HindIII AAGCTT 1 cut(s) 1734
HpaI GTTAAC 2 cut(s) 964, 1656
HpaII CCGG 3 cut(s) 185, 1559, 1791
HphI GGTGA 4 cut(s) 902, 1117, 2051, 2069
Hpy188I TCNGA 4 cut(s) 145, 235, 400, 1018
Hpy188III TCNNGA 6 cut(s) 41, 104, 476, 1003, 1097, 1634
Hpy99I CGWCG 2 cut(s) 312, 2130
HpyAV CCTTC 9 cut(s) 110, 182, 401, 566, 1396, 1584, 1868, 1928, 2044
HpyCH4III ACNGT 7 cut(s) 15, 197, 898, 1080, 1413, 1828, 1833
HpyCH4IV ACGT 1 cut(s) 1652
HpyF10VI GCNNNNNNNGC 8 cut(s) 248, 279, 318, 418, 1210, 1327, 1422, 1913
HpyF3I CTNAG 6 cut(s) 750, 786, 861, 1017, 1253, 1376
HpySE526I ACGT 1 cut(s) 1652
Hsp92I GRCGYC 1 cut(s) 327
HspAI GCGC 6 cut(s) 319, 327, 354, 421, 2119, 2121
KasI GGCGCC 1 cut(s) 326
KflI GGGWCCC 1 cut(s) 1363
KroI GCCGGC 1 cut(s) 1790
KroNI GCCGGC 1 cut(s) 1792
Ksp22I TGATCA 2 cut(s) 1093, 2041
KspAI GTTAAC 2 cut(s) 964, 1656
KspI CCGCGG 1 cut(s) 2073
LguI GCTCTTC 1 cut(s) 767
LmnI GCTCC 7 cut(s) 172, 317, 514, 691, 1318, 1561, 2182
Lsp1109I GCAGC 5 cut(s) 229, 741, 1366, 1607, 1915
LweI GCATC 3 cut(s) 1031, 1466, 1903
MaeI CTAG 1 cut(s) 2102
MaeII ACGT 1 cut(s) 1652
MaeIII GTNAC 5 cut(s) 65, 695, 890, 898, 1405
MbiI CCGCTC 1 cut(s) 1970
MboII GAAGA 7 cut(s) 784, 793, 836, 838, 1280, 2004, 2009
MfeI CAATTG 1 cut(s) 1263
MflI RGATCY 1 cut(s) 1694
MhlI GDGCHC 4 cut(s) 531, 1000, 1385, 2199
MlsI TGGCCA 2 cut(s) 1068, 1118
MluI ACGCGT 1 cut(s) 1742
MluNI TGGCCA 2 cut(s) 1068, 1118
Mly113I GGCGCC 1 cut(s) 327
MlyI GAGTC 4 cut(s) 473, 1135, 1286, 1735
MmeI TCCRAC 3 cut(s) 1242, 1727, 1758
Mox20I TGGCCA 2 cut(s) 1068, 1118
Mph1103I ATGCAT 1 cut(s) 1459
MroNI GCCGGC 1 cut(s) 1790
MscI TGGCCA 2 cut(s) 1068, 1118
MseI TTAA 9 cut(s) 510, 963, 1173, 1482, 1566, 1655, 1860, 2205, 2220
MslI CAYNNNNRTG 4 cut(s) 522, 570, 1242, 1949
Msp20I TGGCCA 2 cut(s) 1068, 1118
MspA1I CMGCKG 6 cut(s) 340, 754, 1474, 1598, 1988, 2072
MspCI CTTAAG 1 cut(s) 1565
MspI CCGG 3 cut(s) 185, 1559, 1791
MspR9I CCNGG 4 cut(s) 185, 316, 1127, 2081
MunI CAATTG 1 cut(s) 1263
Mva1269I GAATGC 5 cut(s) 550, 797, 1184, 1213, 1762
MvaI CCWGG 3 cut(s) 316, 1127, 2081
MvnI CGCG 4 cut(s) 321, 1744, 2072, 2121
MwoI GCNNNNNNNGC 8 cut(s) 248, 279, 318, 418, 1210, 1327, 1422, 1913
NaeI GCCGGC 1 cut(s) 1792
NarI GGCGCC 1 cut(s) 327
NciI CCSGG 1 cut(s) 185
NcoI CCATGG 1 cut(s) 1581
NgoMIV GCCGGC 1 cut(s) 1790
NlaIV GGNNCC 9 cut(s) 142, 328, 628, 734, 792, 1364, 1365, 1384, 1696
NmuCI GTSAC 2 cut(s) 695, 890
NsiI ATGCAT 1 cut(s) 1459
NspI RCATGY 2 cut(s) 304, 1790
NspV TTCGAA 1 cut(s) 228
PaeR7I CTCGAG 1 cut(s) 2114
PagI TCATGA 2 cut(s) 103, 1096
PauI GCGCGC 1 cut(s) 2119
PceI AGGCCT 1 cut(s) 251
PciSI GCTCTTC 1 cut(s) 767
PcsI WCGNNNNNNNCGW 2 cut(s) 565, 1748
PctI GAATGC 5 cut(s) 550, 797, 1184, 1213, 1762
PdiI GCCGGC 1 cut(s) 1792
PfeI GAWTC 6 cut(s) 107, 230, 1013, 1259, 1817, 2138
PfoI TCCNGGA 1 cut(s) 183
PkrI GCNGC 8 cut(s) 244, 275, 756, 1356, 1597, 1848, 1930, 1972
PleI GAGTC 4 cut(s) 473, 1135, 1285, 1734
PluTI GGCGCC 1 cut(s) 330
PpsI GAGTC 4 cut(s) 473, 1135, 1285, 1734
PpuMI RGGWCCY 3 cut(s) 732, 790, 1363
PsiI TTATAA 1 cut(s) 1899
Psp124BI GAGCTC 2 cut(s) 531, 2199
Psp5II RGGWCCY 3 cut(s) 732, 790, 1363
Psp6I CCWGG 3 cut(s) 314, 1125, 2079
PspFI CCCAGC 3 cut(s) 617, 793, 1598
PspGI CCWGG 3 cut(s) 314, 1125, 2079
PspN4I GGNNCC 9 cut(s) 142, 328, 628, 734, 792, 1364, 1365, 1384, 1696
PspPI GGNCC 5 cut(s) 414, 626, 732, 790, 1363
PspPPI RGGWCCY 3 cut(s) 732, 790, 1363
PspXI VCTCGAGB 1 cut(s) 2114
PsrI GAACNNNNNNTAC 2 cut(s) 456, 488
PstI CTGCAG 1 cut(s) 704
PsuI RGATCY 1 cut(s) 1694
PteI GCGCGC 1 cut(s) 2119
PvuII CAGCTG 4 cut(s) 754, 1474, 1598, 1988
RsaI GTAC 4 cut(s) 289, 1373, 1830, 1835
RsaNI GTAC 4 cut(s) 288, 1372, 1829, 1834
RseI CAYNNNNRTG 4 cut(s) 522, 570, 1242, 1949
SacI GAGCTC 2 cut(s) 531, 2199
SacII CCGCGG 1 cut(s) 2073
SapI GCTCTTC 1 cut(s) 767
SaqAI TTAA 9 cut(s) 510, 963, 1173, 1482, 1566, 1655, 1860, 2205, 2220
SatI GCNGC 8 cut(s) 243, 274, 755, 1355, 1596, 1847, 1929, 1971
Sau96I GGNCC 5 cut(s) 414, 626, 732, 790, 1363
SchI GAGTC 4 cut(s) 473, 1135, 1286, 1735
ScrFI CCNGG 4 cut(s) 185, 316, 1127, 2081
SduI GDGCHC 4 cut(s) 531, 1000, 1385, 2199
SfaNI GCATC 3 cut(s) 1031, 1466, 1903
SfcI CTRYAG 2 cut(s) 700, 1469
SfoI GGCGCC 1 cut(s) 328
Sfr274I CTCGAG 1 cut(s) 2114
Sfr303I CCGCGG 1 cut(s) 2073
SfuI TTCGAA 1 cut(s) 228
SgrBI CCGCGG 1 cut(s) 2073
SinI GGWCC 3 cut(s) 732, 790, 1363
SlaI CTCGAG 1 cut(s) 2114
SmiMI CAYNNNNRTG 4 cut(s) 522, 570, 1242, 1949
SmlI CTYRAG 3 cut(s) 1052, 1565, 2114
SmoI CTYRAG 3 cut(s) 1052, 1565, 2114
SseBI AGGCCT 1 cut(s) 251
SsiI CCGC 9 cut(s) 274, 340, 412, 928, 1150, 1846, 1970, 2070, 2072
SspDI GGCGCC 1 cut(s) 326
SspMI CTAG 1 cut(s) 2102
SstI GAGCTC 2 cut(s) 531, 2199
StuI AGGCCT 1 cut(s) 251
StyD4I CCNGG 4 cut(s) 183, 314, 1125, 2079
StyI CCWWGG 5 cut(s) 246, 357, 736, 1581, 1671
TaaI ACNGT 7 cut(s) 15, 197, 898, 1080, 1413, 1828, 1833
TaiI ACGT 1 cut(s) 1655
TaqI TCGA 5 cut(s) 48, 228, 1144, 1337, 2115
TauI GCSGC 3 cut(s) 276, 1849, 1973
TfiI GAWTC 6 cut(s) 107, 230, 1013, 1259, 1817, 2138
Tru1I TTAA 9 cut(s) 510, 963, 1173, 1482, 1566, 1655, 1860, 2205, 2220
Tru9I TTAA 9 cut(s) 510, 963, 1173, 1482, 1566, 1655, 1860, 2205, 2220
TscAI CASTG 9 cut(s) 124, 836, 901, 1083, 1364, 1418, 1423, 1558, 1951
TseFI GTSAC 2 cut(s) 695, 890
TseI GCWGC 5 cut(s) 242, 754, 1354, 1595, 1928
Tsp45I GTSAC 2 cut(s) 695, 890
TspDTI ATGAA 5 cut(s) 92, 837, 1026, 1226, 1442
TspGWI ACGGA 2 cut(s) 704, 2143
TspRI CASTG 9 cut(s) 124, 836, 901, 1083, 1364, 1418, 1423, 1558, 1951
Vha464I CTTAAG 1 cut(s) 1565
VpaK11BI GGWCC 3 cut(s) 732, 790, 1363
XagI CCTNNNNNAGG 1 cut(s) 954
XapI RAATTY 5 cut(s) 44, 224, 1628, 1715, 1893
XceI RCATGY 2 cut(s) 304, 1790
XhoI CTCGAG 1 cut(s) 2114
XspI CTAG 1 cut(s) 2102
Zsp2I ATGCAT 1 cut(s) 1459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.