MD04G1079300.v1.1

Myelodysplasia-myeloid leukemia factor 1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015615
Physical Location & Seq
Forward (+)
10883249 .. 10886323
3075 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1079300.v1.1.491

Sequence Viewer

Length: 912 bp
ATGTCCCCGTTTGGAGGTATGTCTCCATTTGGAGGTATGCCCCCATTTCGAGGGATGTTGGAGTCGAGTGTTTTTGGTCCAAGTGGTGCCAATTTTCCGGATATGCAGCCAAATGGGTTTATTGAGCATCAGGCTCCAGAGCCCAAGAGATCACGGGGCCCTATCATTGAGGAGATAAACTCTGATAATGAAGACGAAGATGCTGACAATGAGAAAAAGGAAAATCCTCGAAAGCGCAGTGGACCGAGCAATGAACCATATGTTGAGGATCCTGATGATGAAGTTGAAGAGAGGAGCAGCAAGCAATTGCAGCATAGGGACAACTACAGCCGGTCTAATTCTATGCAAGCGCAGCCTCAAGCTCACAGCTACACGTTTCAGAGCTCCACCGTCACTTATGGTGGTGCCAATGGCGCATATTATACATCCTCCAAGACAAGGAGGATGGGGAGTGATGGGCTGGCTTTTGAAGAAAGCAAGGAAGCTGATAGTACTTCTAGGCAAGCAACTCACAGGGTATCAAGAGGGCTCCACAATAAGGGCCATTCAGTTACGAGGAAGCTCAAAACGGATGGTAAGGTGGATACAATGCAGACCTTGCACAATCTTAATGAAGATGAGCTTTCCGGGTTTGAAGAAGCATGGAAAGGAACTGCTAAGAAACATTTGCCTGTATTGGCTGATAAATTTGGTGGATATGAAAATATAGGAGCCGGCAGCAGCGGACATGGGCAGGCTAGTCGGCAAGGTTGGGCGCTTCCATCCAATGAGCATGTACAGAACCCCGGCAATATGATTGAAGATGCTAGAGACAAGGCGGGAGGATCTTCTCACTCAAACAAGTCTGGTAGTAGGATGAGAGCAGATGTAAAGGACACGAGTGGCTATTCCCGTGGGAGAGGAAGAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 map05202
Pfam Domains
Protein Families
Mlf1IP

Protein Analysis

304

Amino Acids

33.14

Weight (kDa)

6.28

Isoelectric Point (pI)

58.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlf1IP PF10248 69 - 217 1.3e-21 Myelodysplasia-myeloid leukemia factor 1-interacting protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015615)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g00720
malus_domestica MD04G1079300.v1.1
prunus_persica Prupe.5G079800_v2.0.a1
pyrus_communis pycom04g07110
rosa_chinensis RchiOBHm_Chr7g0201491
rosa_laevigata RLG00000003669
rosa_multiflora Rmu_co8397869.1_g000001 Rmu_sc0002046.1_g000005 Rmu_sc0017656.1_g000004
rosa_roxburghii Rroxscaffold_3G00255260
rosa_rugosa Rorug07G0064300
rosa_samantha Rh7AG190900 Rh7BG193200 Rh7CG202300 Rh7DG195900
rosa_wichuraiana Rw7G016740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 86, 404
AccIII TCCGGA 1 cut(s) 97
AciI CCGC 2 cut(s) 723, 818
AclWI GGATC 3 cut(s) 263, 276, 832
AcsI RAATTY 1 cut(s) 686
AfaI GTAC 2 cut(s) 493, 777
AfiI CCNNNNNNNGG 5 cut(s) 14, 32, 50, 438, 538
AflIII ACRYGT 1 cut(s) 372
AgsI TTSAA 4 cut(s) 287, 470, 635, 800
AluBI AGCT 6 cut(s) 362, 369, 384, 485, 562, 622
AluI AGCT 6 cut(s) 362, 369, 384, 485, 562, 622
Alw21I GWGCWC 1 cut(s) 386
Alw26I GTCTC 2 cut(s) 27, 804
AlwI GGATC 3 cut(s) 263, 276, 832
Aor13HI TCCGGA 1 cut(s) 97
AoxI GGCC 2 cut(s) 157, 541
ApaI GGGCCC 1 cut(s) 161
ApeKI GCWGC 6 cut(s) 106, 297, 310, 352, 717, 720
ApoI RAATTY 1 cut(s) 686
AspLEI GCGC 4 cut(s) 237, 352, 416, 757
AspS9I GGNCC 5 cut(s) 77, 157, 158, 242, 541
AsuC2I CCSGG 2 cut(s) 628, 786
AvaII GGWCC 2 cut(s) 77, 242
BaeGI GKGCMC 1 cut(s) 161
BamHI GGATCC 1 cut(s) 268
BanI GGYRCC 2 cut(s) 86, 404
BanII GRGCYC 4 cut(s) 144, 161, 386, 531
BauI CACGAG 1 cut(s) 877
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 386
BbvI GCAGC 6 cut(s) 118, 309, 322, 364, 729, 732
BccI CCATC 4 cut(s) 439, 449, 566, 769
BcgI CGANNNNNNTGC 2 cut(s) 722, 756
BciVI GTATCC 1 cut(s) 577
BcnI CCSGG 2 cut(s) 628, 786
BcoDI GTCTC 2 cut(s) 27, 804
BfaI CTAG 3 cut(s) 498, 738, 807
BfmI CTRYAG 1 cut(s) 325
BfoI RGCGCY 1 cut(s) 758
BfuI GTATCC 1 cut(s) 577
BisI GCNGC 6 cut(s) 107, 298, 311, 353, 718, 721
BlsI GCNGC 6 cut(s) 108, 299, 312, 354, 719, 722
BmcAI AGTACT 1 cut(s) 493
Bme1390I CCNGG 2 cut(s) 628, 786
Bme18I GGWCC 2 cut(s) 77, 242
BmgT120I GGNCC 5 cut(s) 77, 157, 158, 242, 541
BmiI GGNNCC 8 cut(s) 88, 135, 158, 159, 270, 406, 530, 712
BmrFI CCNGG 2 cut(s) 628, 786
BmsI GCATC 3 cut(s) 136, 190, 793
BpiI GAAGAC 1 cut(s) 198
BplI GAGNNNNNCTC 2 cut(s) 164, 196
BpmI CTGGAG 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 342
BpuMI CCSGG 2 cut(s) 628, 786
BsaJI CCNNGG 2 cut(s) 784, 892
BsaWI WCCGGW 1 cut(s) 97
Bsc4I CCNNNNNNNGG 5 cut(s) 14, 32, 50, 438, 538
Bse118I RCCGGY 2 cut(s) 330, 713
Bse3DI GCAATG 1 cut(s) 256
BseAI TCCGGA 1 cut(s) 97
BseDI CCNNGG 2 cut(s) 784, 892
BseGI GGATG 6 cut(s) 60, 425, 450, 577, 761, 861
BseLI CCNNNNNNNGG 5 cut(s) 14, 32, 50, 438, 538
BseMI GCAATG 1 cut(s) 256
BseRI GAGGAG 2 cut(s) 185, 307
BseSI GKGCMC 1 cut(s) 161
BseXI GCAGC 6 cut(s) 118, 309, 322, 364, 729, 732
BshFI GGCC 2 cut(s) 159, 543
BshNI GGYRCC 2 cut(s) 86, 404
BsiHKAI GWGCWC 1 cut(s) 386
BsiSI CCGG 5 cut(s) 98, 331, 627, 714, 786
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 5 cut(s) 14, 32, 50, 438, 538
BsmAI GTCTC 2 cut(s) 27, 804
BsmFI GGGAC 1 cut(s) 332
BsnI GGCC 2 cut(s) 159, 543
Bsp120I GGGCCC 1 cut(s) 157
Bsp1286I GDGCHC 4 cut(s) 144, 161, 386, 531
Bsp13I TCCGGA 1 cut(s) 97
Bsp1407I TGTACA 1 cut(s) 775
Bsp143I GATC 3 cut(s) 149, 268, 824
BspACI CCGC 2 cut(s) 723, 818
BspANI GGCC 2 cut(s) 159, 543
BspEI TCCGGA 1 cut(s) 97
BspLI GGNNCC 8 cut(s) 88, 135, 158, 159, 270, 406, 530, 712
BspPI GGATC 3 cut(s) 263, 276, 832
BspQI GCTCTTC 1 cut(s) 898
BspT107I GGYRCC 2 cut(s) 86, 404
BsrDI GCAATG 1 cut(s) 256
BsrFI RCCGGY 2 cut(s) 330, 713
BsrGI TGTACA 1 cut(s) 775
BssAI RCCGGY 2 cut(s) 330, 713
BssECI CCNNGG 2 cut(s) 784, 892
BssMI GATC 3 cut(s) 149, 268, 824
BssSI CACGAG 1 cut(s) 877
Bst2BI CACGAG 1 cut(s) 877
Bst4CI ACNGT 1 cut(s) 391
Bst6I CTCTTC 2 cut(s) 282, 898
BstAPI GCANNNNNTGC 1 cut(s) 598
BstAUI TGTACA 1 cut(s) 775
BstC8I GCNNGC 6 cut(s) 302, 348, 462, 504, 715, 735
BstDEI CTNAG 1 cut(s) 657
BstDSI CCRYGG 1 cut(s) 892
BstF5I GGATG 6 cut(s) 60, 425, 450, 577, 761, 861
BstH2I RGCGCY 1 cut(s) 758
BstHHI GCGC 4 cut(s) 237, 352, 416, 757
BstKTI GATC 3 cut(s) 152, 271, 827
BstMAI GTCTC 2 cut(s) 27, 804
BstMBI GATC 3 cut(s) 149, 268, 824
BstMWI GCNNNNNNNGC 4 cut(s) 310, 352, 413, 598
BstNSI RCATGY 1 cut(s) 776
BstSCI CCNGG 2 cut(s) 626, 784
BstSFI CTRYAG 1 cut(s) 325
BstSLI GKGCMC 1 cut(s) 161
BstV1I GCAGC 6 cut(s) 118, 309, 322, 364, 729, 732
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 2 cut(s) 268, 824
BstYI RGATCY 2 cut(s) 268, 824
BsuI GTATCC 1 cut(s) 577
BsuRI GGCC 2 cut(s) 159, 543
BtgI CCRYGG 1 cut(s) 892
BtsCI GGATG 6 cut(s) 60, 425, 450, 577, 761, 861
BtsI GCAGTG 1 cut(s) 244
BtsIMutI CAGTG 1 cut(s) 244
Cac8I GCNNGC 6 cut(s) 302, 348, 462, 504, 715, 735
CfoI GCGC 4 cut(s) 237, 352, 416, 757
Cfr10I RCCGGY 2 cut(s) 330, 713
Cfr13I GGNCC 5 cut(s) 77, 157, 158, 242, 541
Csp6I GTAC 2 cut(s) 492, 776
CviAII CATG 3 cut(s) 642, 728, 773
CviQI GTAC 2 cut(s) 492, 776
DdeI CTNAG 1 cut(s) 657
DpnI GATC 3 cut(s) 151, 270, 826
DpnII GATC 3 cut(s) 149, 268, 824
Eam1104I CTCTTC 2 cut(s) 282, 898
EarI CTCTTC 2 cut(s) 282, 898
Ecl136II GAGCTC 1 cut(s) 384
Eco24I GRGCYC 4 cut(s) 144, 161, 386, 531
Eco47I GGWCC 2 cut(s) 77, 242
Eco53kI GAGCTC 1 cut(s) 384
EcoICRI GAGCTC 1 cut(s) 384
EcoO109I RGGNCCY 2 cut(s) 157, 158
EcoT38I GRGCYC 4 cut(s) 144, 161, 386, 531
FaeI CATG 3 cut(s) 645, 731, 776
FalI AAGNNNNNCTT 2 cut(s) 606, 638
FaqI GGGAC 1 cut(s) 332
FatI CATG 3 cut(s) 641, 727, 772
FauI CCCGC 1 cut(s) 811
FauNDI CATATG 1 cut(s) 259
Fnu4HI GCNGC 6 cut(s) 107, 298, 311, 353, 718, 721
FokI GGATG 6 cut(s) 67, 412, 457, 584, 748, 868
FriOI GRGCYC 4 cut(s) 144, 161, 386, 531
Fsp4HI GCNGC 6 cut(s) 107, 298, 311, 353, 718, 721
FspBI CTAG 3 cut(s) 498, 738, 807
GlaI GCGC 4 cut(s) 236, 351, 415, 756
GluI GCNGC 6 cut(s) 107, 298, 311, 353, 718, 721
GsuI CTGGAG 1 cut(s) 120
HaeII RGCGCY 1 cut(s) 758
HaeIII GGCC 2 cut(s) 159, 543
HapII CCGG 5 cut(s) 98, 331, 627, 714, 786
HhaI GCGC 4 cut(s) 237, 352, 416, 757
Hin1II CATG 3 cut(s) 645, 731, 776
Hin6I GCGC 4 cut(s) 235, 350, 414, 755
HinP1I GCGC 4 cut(s) 235, 350, 414, 755
HinfI GANTC 1 cut(s) 62
HpaII CCGG 5 cut(s) 98, 331, 627, 714, 786
Hpy166II GTNNAC 1 cut(s) 242
Hpy188I TCNGA 2 cut(s) 184, 381
Hpy188III TCNNGA 4 cut(s) 98, 137, 272, 522
Hpy8I GTNNAC 1 cut(s) 242
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4IV ACGT 1 cut(s) 374
HpyCH4V TGCA 5 cut(s) 106, 310, 346, 592, 601
HpyF10VI GCNNNNNNNGC 4 cut(s) 310, 352, 413, 598
HpyF3I CTNAG 1 cut(s) 657
HpySE526I ACGT 1 cut(s) 374
Hsp92II CATG 3 cut(s) 645, 731, 776
HspAI GCGC 4 cut(s) 235, 350, 414, 755
Kpn2I TCCGGA 1 cut(s) 97
KroI GCCGGC 1 cut(s) 713
KroNI GCCGGC 1 cut(s) 715
Kzo9I GATC 3 cut(s) 149, 268, 824
LguI GCTCTTC 1 cut(s) 898
LmnI GCTCC 5 cut(s) 139, 294, 389, 534, 710
Lsp1109I GCAGC 6 cut(s) 118, 309, 322, 364, 729, 732
LweI GCATC 3 cut(s) 136, 190, 793
MaeI CTAG 3 cut(s) 498, 738, 807
MaeII ACGT 1 cut(s) 374
MaeIII GTNAC 2 cut(s) 391, 550
MalI GATC 3 cut(s) 151, 270, 826
MboI GATC 3 cut(s) 149, 268, 824
MboII GAAGA 8 cut(s) 203, 209, 299, 482, 626, 647, 812, 819
MfeI CAATTG 1 cut(s) 305
MflI RGATCY 2 cut(s) 268, 824
MhlI GDGCHC 4 cut(s) 144, 161, 386, 531
MluCI AATT 4 cut(s) 91, 305, 337, 686
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 1 cut(s) 39
MroI TCCGGA 1 cut(s) 97
MroNI GCCGGC 1 cut(s) 713
MseI TTAA 1 cut(s) 609
MspA1I CMGCKG 1 cut(s) 723
MspI CCGG 5 cut(s) 98, 331, 627, 714, 786
MspR9I CCNGG 2 cut(s) 628, 786
MunI CAATTG 1 cut(s) 305
MwoI GCNNNNNNNGC 4 cut(s) 310, 352, 413, 598
NaeI GCCGGC 1 cut(s) 715
NciI CCSGG 2 cut(s) 628, 786
NdeI CATATG 1 cut(s) 259
NdeII GATC 3 cut(s) 149, 268, 824
NgoMIV GCCGGC 1 cut(s) 713
NlaIII CATG 3 cut(s) 645, 731, 776
NlaIV GGNNCC 8 cut(s) 88, 135, 158, 159, 270, 406, 530, 712
NmuCI GTSAC 1 cut(s) 391
NspI RCATGY 1 cut(s) 776
PciSI GCTCTTC 1 cut(s) 898
PdiI GCCGGC 1 cut(s) 715
PkrI GCNGC 6 cut(s) 108, 299, 312, 354, 719, 722
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
Psp124BI GAGCTC 1 cut(s) 386
PspN4I GGNNCC 8 cut(s) 88, 135, 158, 159, 270, 406, 530, 712
PspOMI GGGCCC 1 cut(s) 157
PspPI GGNCC 5 cut(s) 77, 157, 158, 242, 541
PsuI RGATCY 2 cut(s) 268, 824
RsaI GTAC 2 cut(s) 493, 777
RsaNI GTAC 2 cut(s) 492, 776
SacI GAGCTC 1 cut(s) 386
SapI GCTCTTC 1 cut(s) 898
SaqAI TTAA 1 cut(s) 609
SatI GCNGC 6 cut(s) 107, 298, 311, 353, 718, 721
Sau3AI GATC 3 cut(s) 149, 268, 824
Sau96I GGNCC 5 cut(s) 77, 157, 158, 242, 541
ScaI AGTACT 1 cut(s) 493
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 2 cut(s) 628, 786
SduI GDGCHC 4 cut(s) 144, 161, 386, 531
SfaNI GCATC 3 cut(s) 136, 190, 793
SfcI CTRYAG 1 cut(s) 325
SinI GGWCC 2 cut(s) 77, 242
SmlI CTYRAG 1 cut(s) 357
SmoI CTYRAG 1 cut(s) 357
Sse9I AATT 4 cut(s) 91, 305, 337, 686
SsiI CCGC 2 cut(s) 723, 818
SspMI CTAG 3 cut(s) 498, 738, 807
SstI GAGCTC 1 cut(s) 386
StyD4I CCNGG 2 cut(s) 626, 784
TaaI ACNGT 1 cut(s) 391
TaiI ACGT 1 cut(s) 377
TaqI TCGA 3 cut(s) 49, 65, 229
TaqII GACCGA 1 cut(s) 259
TasI AATT 4 cut(s) 91, 305, 337, 686
TatI WGTACW 2 cut(s) 491, 775
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TscAI CASTG 1 cut(s) 244
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 6 cut(s) 106, 297, 310, 352, 717, 720
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 5 cut(s) 204, 267, 294, 627, 714
TspGWI ACGGA 1 cut(s) 584
TspRI CASTG 1 cut(s) 244
VpaK11BI GGWCC 2 cut(s) 77, 242
XapI RAATTY 1 cut(s) 686
XceI RCATGY 1 cut(s) 776
XspI CTAG 3 cut(s) 498, 738, 807
ZrmI AGTACT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.