Prupe.5G079800_v2.0.a1

Myelodysplasia-myeloid leukemia factor 1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0015615
Physical Location & Seq
Reverse (-)
9359634 .. 9362950
3317 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G079800.1

Sequence Viewer

Length: 1116 bp
ATGCAGGGGGGCAGAGGCGGTCCAGGTGGTAGGGGTCCTTTCTCTGATTTCAATGATCCGTTTGCTGGTTTTGGGGGGTTTGGTGGATTTGGGGGTCAGAGGAGTTTGATTTCTAACCTTTTTGGAGGAAGGGATCCATTTGATGACCCTTTCTTCACTCGCCCATTTGGAGGTATGTCCCCGTTGGGAGGTATGGCCCCGTTGGGAGGTATGTCCCCGTTTGGAGGTATGCCCCCGTTTGGAGGTATGCCCCCATTTGGAGGGATGTTGGAGTCGAGTTTTTTTGGTCCAGGTGGAGGCAATTTTCCAGATATGCAGCCAACTGGGTTCATTGAGCATCAGGCTCCAGAGCCTAAGAGACCAAGGGGCCCAATCATTGAGGAAATAGACTCTGATCATGAAAATGAAGATGCTGATAATGAGAAAAAAGAAAATCCTCGAAAACGCAGTGGACCGAGCAATGAACCTTATGTTGAGGATCCTGATGATGAAGTTGAAGAGAAGAGCAGCAAGCATTTGCAGCATAGGGACAACTATAACCAGTATAATGCTATGCAATCCCAGCCTCAAGCTCAGAGCTTCACATTTCAGAGCTCCAGTGTCTCTTATGGTGGTGCCAATGGTGCATATTATACTTCATCCAAGACAAGAAGGATGGGGAGTGATGGGTTGGCTTTTGAAGAAAGTAAGGAAGCTGATACTACTTCTGGGAAGGCAACACACAGGGTATCTAAGGGGCTCCGCAATAAGGGCCATTCAATTACTAGGAAGCTCAAAACAGACGGTAAGGTGGATACAATGCAGACCTTGCACAATCTTAACGAAGATGAGATTTCCGGTTTTGAAGAAGCATGGAAAGGAACTGCTAAAAAACGTTTGCCTGGATGGACTGATAAATTTGGTGGTTACGAAAATCTAGGAGCTAGCAGCAGTGGACGTGAGGAGGCTAGTCGGGGAGGTTGGGCGCTTCCTTCCAACGAGAGTTCACAGAACCCTGGAAATATGATGTCAGCTGCCAAAGAGAAGGCAGGATCTTCTCGCTCGAACCAGTCTGGTAGGATGAGAGCAGATGTTAGGGACAAGAATGGTTATTCCCGTGGGAAAGCAAGAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 map05202
Pfam Domains
Protein Families
Mlf1IP

Protein Analysis

372

Amino Acids

40.02

Weight (kDa)

6.22

Isoelectric Point (pI)

59.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015615)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g00720
malus_domestica MD04G1079300.v1.1
prunus_persica Prupe.5G079800_v2.0.a1
pyrus_communis pycom04g07110
rosa_chinensis RchiOBHm_Chr7g0201491
rosa_laevigata RLG00000003669
rosa_multiflora Rmu_co8397869.1_g000001 Rmu_sc0002046.1_g000005 Rmu_sc0017656.1_g000004
rosa_roxburghii Rroxscaffold_3G00255260
rosa_rugosa Rorug07G0064300
rosa_samantha Rh7AG190900 Rh7BG193200 Rh7CG202300 Rh7DG195900
rosa_wichuraiana Rw7G016740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 614
AciI CCGC 2 cut(s) 18, 742
AclI AACGTT 1 cut(s) 874
AclWI GGATC 6 cut(s) 50, 128, 141, 473, 486, 1039
AcsI RAATTY 1 cut(s) 896
AgsI TTSAA 5 cut(s) 52, 497, 680, 759, 845
AjiI CACGTC 1 cut(s) 938
AjnI CCWGG 4 cut(s) 22, 289, 880, 994
AluBI AGCT 7 cut(s) 572, 579, 594, 695, 772, 923, 1013
AluI AGCT 7 cut(s) 572, 579, 594, 695, 772, 923, 1013
Alw21I GWGCWC 1 cut(s) 596
Alw26I GTCTC 2 cut(s) 352, 607
AlwI GGATC 6 cut(s) 50, 128, 141, 473, 486, 1039
AoxI GGCC 3 cut(s) 195, 367, 751
ApaI GGGCCC 1 cut(s) 371
ApeKI GCWGC 5 cut(s) 316, 507, 520, 927, 1013
ApoI RAATTY 1 cut(s) 896
AspLEI GCGC 1 cut(s) 967
AspS9I GGNCC 8 cut(s) 20, 35, 196, 287, 367, 368, 452, 751
AsuNHI GCTAGC 1 cut(s) 923
AvaII GGWCC 4 cut(s) 20, 35, 287, 452
BaeGI GKGCMC 1 cut(s) 371
BamHI GGATCC 2 cut(s) 133, 478
BanI GGYRCC 1 cut(s) 614
BanII GRGCYC 3 cut(s) 371, 596, 741
Bbv12I GWGCWC 1 cut(s) 596
BbvI GCAGC 5 cut(s) 328, 519, 532, 939, 1000
BccI CCATC 3 cut(s) 649, 659, 879
BciT130I CCWGG 4 cut(s) 24, 291, 882, 996
BciVI GTATCC 1 cut(s) 787
BclI TGATCA 1 cut(s) 394
BcoDI GTCTC 2 cut(s) 352, 607
BfaI CTAG 4 cut(s) 765, 917, 924, 948
BfoI RGCGCY 1 cut(s) 968
BfuI GTATCC 1 cut(s) 787
BisI GCNGC 5 cut(s) 317, 508, 521, 928, 1014
BlsI GCNGC 5 cut(s) 318, 509, 522, 929, 1015
Bme1390I CCNGG 4 cut(s) 24, 291, 882, 996
Bme18I GGWCC 4 cut(s) 20, 35, 287, 452
BmgBI CACGTC 1 cut(s) 938
BmgT120I GGNCC 8 cut(s) 20, 35, 196, 287, 367, 368, 452, 751
BmiI GGNNCC 9 cut(s) 36, 135, 198, 345, 368, 369, 480, 616, 740
BmrFI CCNGG 4 cut(s) 24, 291, 882, 996
BmrI ACTGGG 1 cut(s) 333
BmsI GCATC 2 cut(s) 346, 400
BmtI GCTAGC 1 cut(s) 927
BmuI ACTGGG 1 cut(s) 333
BpmI CTGGAG 2 cut(s) 330, 580
BpuEI CTTGAG 1 cut(s) 552
BsaI GGTCTC 1 cut(s) 352
BsaJI CCNNGG 3 cut(s) 362, 994, 1096
BsaWI WCCGGW 1 cut(s) 836
Bse1I ACTGG 4 cut(s) 328, 541, 597, 1048
Bse3DI GCAATG 1 cut(s) 466
BseBI CCWGG 4 cut(s) 24, 291, 882, 996
BseDI CCNNGG 3 cut(s) 362, 994, 1096
BseGI GGATG 5 cut(s) 270, 638, 660, 890, 1065
BseMI GCAATG 1 cut(s) 466
BseMII CTCAG 1 cut(s) 587
BseNI ACTGG 4 cut(s) 328, 541, 597, 1048
BseRI GAGGAG 2 cut(s) 115, 956
BseSI GKGCMC 1 cut(s) 371
BseXI GCAGC 5 cut(s) 328, 519, 532, 939, 1000
BseYI CCCAGC 1 cut(s) 561
BshFI GGCC 3 cut(s) 197, 369, 753
BshNI GGYRCC 1 cut(s) 614
BsiHKAI GWGCWC 1 cut(s) 596
BsiSI CCGG 1 cut(s) 837
BslFI GGGAC 4 cut(s) 163, 199, 542, 1091
BsmAI GTCTC 2 cut(s) 352, 607
BsmFI GGGAC 4 cut(s) 163, 199, 542, 1091
BsnI GGCC 3 cut(s) 197, 369, 753
Bso31I GGTCTC 1 cut(s) 352
Bsp120I GGGCCC 1 cut(s) 367
Bsp1286I GDGCHC 3 cut(s) 371, 596, 741
Bsp143I GATC 5 cut(s) 55, 133, 394, 478, 1031
BspACI CCGC 2 cut(s) 18, 742
BspANI GGCC 3 cut(s) 197, 369, 753
BspCNI CTCAG 1 cut(s) 586
BspHI TCATGA 1 cut(s) 397
BspLI GGNNCC 9 cut(s) 36, 135, 198, 345, 368, 369, 480, 616, 740
BspOI GCTAGC 1 cut(s) 927
BspPI GGATC 6 cut(s) 50, 128, 141, 473, 486, 1039
BspQI GCTCTTC 1 cut(s) 497
BspT107I GGYRCC 1 cut(s) 614
BspTNI GGTCTC 1 cut(s) 352
BsrDI GCAATG 1 cut(s) 466
BsrI ACTGG 4 cut(s) 328, 541, 597, 1048
BssECI CCNNGG 3 cut(s) 362, 994, 1096
BssMI GATC 5 cut(s) 55, 133, 394, 478, 1031
BssT1I CCWWGG 1 cut(s) 362
Bst2UI CCWGG 4 cut(s) 24, 291, 882, 996
Bst4CI ACNGT 1 cut(s) 785
Bst6I CTCTTC 2 cut(s) 492, 497
BstAPI GCANNNNNTGC 1 cut(s) 808
BstC8I GCNNGC 2 cut(s) 512, 925
BstDEI CTNAG 3 cut(s) 354, 573, 732
BstDSI CCRYGG 1 cut(s) 1096
BstF5I GGATG 5 cut(s) 270, 638, 660, 890, 1065
BstH2I RGCGCY 1 cut(s) 968
BstHHI GCGC 1 cut(s) 967
BstKTI GATC 5 cut(s) 58, 136, 397, 481, 1034
BstMAI GTCTC 2 cut(s) 352, 607
BstMBI GATC 5 cut(s) 55, 133, 394, 478, 1031
BstMWI GCNNNNNNNGC 5 cut(s) 520, 562, 623, 750, 808
BstNI CCWGG 4 cut(s) 24, 291, 882, 996
BstSCI CCNGG 4 cut(s) 22, 289, 880, 994
BstSLI GKGCMC 1 cut(s) 371
BstV1I GCAGC 5 cut(s) 328, 519, 532, 939, 1000
BstX2I RGATCY 3 cut(s) 133, 478, 1031
BstYI RGATCY 3 cut(s) 133, 478, 1031
BsuI GTATCC 1 cut(s) 787
BsuRI GGCC 3 cut(s) 197, 369, 753
BtgI CCRYGG 1 cut(s) 1096
BtrI CACGTC 1 cut(s) 938
BtsCI GGATG 5 cut(s) 270, 638, 660, 890, 1065
BtsI GCAGTG 2 cut(s) 454, 937
BtsIMutI CAGTG 3 cut(s) 454, 604, 937
Cac8I GCNNGC 2 cut(s) 512, 925
CciI TCATGA 1 cut(s) 397
CfoI GCGC 1 cut(s) 967
Cfr13I GGNCC 8 cut(s) 20, 35, 196, 287, 367, 368, 452, 751
CviAII CATG 2 cut(s) 398, 852
DdeI CTNAG 3 cut(s) 354, 573, 732
DpnI GATC 5 cut(s) 57, 135, 396, 480, 1033
DpnII GATC 5 cut(s) 55, 133, 394, 478, 1031
Eam1104I CTCTTC 2 cut(s) 492, 497
EarI CTCTTC 2 cut(s) 492, 497
Ecl136II GAGCTC 1 cut(s) 594
Eco130I CCWWGG 1 cut(s) 362
Eco24I GRGCYC 3 cut(s) 371, 596, 741
Eco31I GGTCTC 1 cut(s) 352
Eco47I GGWCC 4 cut(s) 20, 35, 287, 452
Eco53kI GAGCTC 1 cut(s) 594
EcoICRI GAGCTC 1 cut(s) 594
EcoO109I RGGNCCY 2 cut(s) 35, 367
EcoRII CCWGG 4 cut(s) 22, 289, 880, 994
EcoT14I CCWWGG 1 cut(s) 362
EcoT38I GRGCYC 3 cut(s) 371, 596, 741
ErhI CCWWGG 1 cut(s) 362
FaeI CATG 2 cut(s) 401, 855
FaqI GGGAC 4 cut(s) 163, 199, 542, 1091
FatI CATG 2 cut(s) 397, 851
FbaI TGATCA 1 cut(s) 394
Fnu4HI GCNGC 5 cut(s) 317, 508, 521, 928, 1014
FokI GGATG 5 cut(s) 277, 625, 667, 897, 1072
FriOI GRGCYC 3 cut(s) 371, 596, 741
Fsp4HI GCNGC 5 cut(s) 317, 508, 521, 928, 1014
FspBI CTAG 4 cut(s) 765, 917, 924, 948
GlaI GCGC 1 cut(s) 966
GluI GCNGC 5 cut(s) 317, 508, 521, 928, 1014
GsaI CCCAGC 1 cut(s) 565
GsuI CTGGAG 2 cut(s) 330, 580
HaeII RGCGCY 1 cut(s) 968
HaeIII GGCC 3 cut(s) 197, 369, 753
HapII CCGG 1 cut(s) 837
HhaI GCGC 1 cut(s) 967
Hin1II CATG 2 cut(s) 401, 855
Hin6I GCGC 1 cut(s) 965
HinP1I GCGC 1 cut(s) 965
HinfI GANTC 2 cut(s) 272, 389
HpaII CCGG 1 cut(s) 837
Hpy166II GTNNAC 3 cut(s) 452, 935, 986
Hpy188I TCNGA 5 cut(s) 46, 99, 394, 576, 591
Hpy188III TCNNGA 4 cut(s) 308, 347, 398, 482
Hpy8I GTNNAC 3 cut(s) 452, 935, 986
HpyAV CCTTC 5 cut(s) 123, 645, 706, 981, 1018
HpyCH4III ACNGT 1 cut(s) 785
HpyCH4IV ACGT 2 cut(s) 874, 937
HpyCH4V TGCA 7 cut(s) 4, 316, 520, 556, 626, 802, 811
HpyF10VI GCNNNNNNNGC 5 cut(s) 520, 562, 623, 750, 808
HpyF3I CTNAG 3 cut(s) 354, 573, 732
HpySE526I ACGT 2 cut(s) 874, 937
Hsp92II CATG 2 cut(s) 401, 855
HspAI GCGC 1 cut(s) 965
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 5 cut(s) 55, 133, 394, 478, 1031
LguI GCTCTTC 1 cut(s) 497
LmnI GCTCC 4 cut(s) 349, 599, 744, 920
Lsp1109I GCAGC 5 cut(s) 328, 519, 532, 939, 1000
LweI GCATC 2 cut(s) 346, 400
MaeI CTAG 4 cut(s) 765, 917, 924, 948
MaeII ACGT 2 cut(s) 874, 937
MaeIII GTNAC 1 cut(s) 905
MalI GATC 5 cut(s) 57, 135, 396, 480, 1033
MboI GATC 5 cut(s) 55, 133, 394, 478, 1031
MboII GAAGA 8 cut(s) 145, 419, 509, 514, 692, 836, 857, 1026
MflI RGATCY 3 cut(s) 133, 478, 1031
MhlI GDGCHC 3 cut(s) 371, 596, 741
MluCI AATT 3 cut(s) 301, 759, 896
MlyI GAGTC 2 cut(s) 281, 383
MmeI TCCRAC 2 cut(s) 249, 999
MseI TTAA 1 cut(s) 819
MslI CAYNNNNRTG 1 cut(s) 402
MspA1I CMGCKG 1 cut(s) 1013
MspI CCGG 1 cut(s) 837
MspR9I CCNGG 4 cut(s) 24, 291, 882, 996
MvaI CCWGG 4 cut(s) 24, 291, 882, 996
MwoI GCNNNNNNNGC 5 cut(s) 520, 562, 623, 750, 808
NdeII GATC 5 cut(s) 55, 133, 394, 478, 1031
NheI GCTAGC 1 cut(s) 923
NlaIII CATG 2 cut(s) 401, 855
NlaIV GGNNCC 9 cut(s) 36, 135, 198, 345, 368, 369, 480, 616, 740
PagI TCATGA 1 cut(s) 397
PciSI GCTCTTC 1 cut(s) 497
PkrI GCNGC 5 cut(s) 318, 509, 522, 929, 1015
PleI GAGTC 2 cut(s) 280, 383
PpsI GAGTC 2 cut(s) 280, 383
PpuMI RGGWCCY 1 cut(s) 35
Psp124BI GAGCTC 1 cut(s) 596
Psp1406I AACGTT 1 cut(s) 874
Psp5II RGGWCCY 1 cut(s) 35
Psp6I CCWGG 4 cut(s) 22, 289, 880, 994
PspFI CCCAGC 1 cut(s) 561
PspGI CCWGG 4 cut(s) 22, 289, 880, 994
PspN4I GGNNCC 9 cut(s) 36, 135, 198, 345, 368, 369, 480, 616, 740
PspOMI GGGCCC 1 cut(s) 367
PspPI GGNCC 8 cut(s) 20, 35, 196, 287, 367, 368, 452, 751
PspPPI RGGWCCY 1 cut(s) 35
PsuI RGATCY 3 cut(s) 133, 478, 1031
PvuII CAGCTG 1 cut(s) 1013
RseI CAYNNNNRTG 1 cut(s) 402
SacI GAGCTC 1 cut(s) 596
SapI GCTCTTC 1 cut(s) 497
SaqAI TTAA 1 cut(s) 819
SatI GCNGC 5 cut(s) 317, 508, 521, 928, 1014
Sau3AI GATC 5 cut(s) 55, 133, 394, 478, 1031
Sau96I GGNCC 8 cut(s) 20, 35, 196, 287, 367, 368, 452, 751
SchI GAGTC 2 cut(s) 281, 383
ScrFI CCNGG 4 cut(s) 24, 291, 882, 996
SduI GDGCHC 3 cut(s) 371, 596, 741
SfaNI GCATC 2 cut(s) 346, 400
SinI GGWCC 4 cut(s) 20, 35, 287, 452
SmiMI CAYNNNNRTG 1 cut(s) 402
SmlI CTYRAG 1 cut(s) 567
SmoI CTYRAG 1 cut(s) 567
Sse9I AATT 3 cut(s) 301, 759, 896
SsiI CCGC 2 cut(s) 18, 742
SspMI CTAG 4 cut(s) 765, 917, 924, 948
SstI GAGCTC 1 cut(s) 596
StyD4I CCNGG 4 cut(s) 22, 289, 880, 994
StyI CCWWGG 1 cut(s) 362
TaaI ACNGT 1 cut(s) 785
TaiI ACGT 2 cut(s) 877, 940
TaqI TCGA 3 cut(s) 275, 439, 1043
TaqII GACCGA 1 cut(s) 469
TasI AATT 3 cut(s) 301, 759, 896
Tru1I TTAA 1 cut(s) 819
Tru9I TTAA 1 cut(s) 819
TscAI CASTG 3 cut(s) 454, 604, 937
TseI GCWGC 5 cut(s) 316, 507, 520, 927, 1013
TspDTI ATGAA 6 cut(s) 319, 414, 420, 477, 504, 627
TspGWI ACGGA 1 cut(s) 48
TspRI CASTG 3 cut(s) 454, 604, 937
VpaK11BI GGWCC 4 cut(s) 20, 35, 287, 452
XapI RAATTY 1 cut(s) 896
XspI CTAG 4 cut(s) 765, 917, 924, 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.