Rroxscaffold_3G00255260

Myelodysplasia-myeloid leukemia factor 1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0015615
Physical Location & Seq
Reverse (-)
49490926 .. 49493963
3038 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00255260.1

Sequence Viewer

Length: 1095 bp
ATGCAAGGGGGAAGAGGTGGAGGACCTGGTGGCAGGGGTCCTTTTGATTTCAATGATCCTTTTGCTGGTTTTGGGGGATTTGGGCGGCAGGGGAGTTTGATGTCTAACGTCTTCGGAGGGAGGGATCCGTTTGATGACCCTTTCTTTACTCGCCCGTTTGGAGGGATATTTGACTCGGGTTTTCCGGGTATGGGTGGAGGCATGTTTCCTGGAATGGGTGGCAGTTTGTTTCCTGGAATGGGTGGCGGTATGTTTCCTGGAATGGGTCCTGGTATGAATGGAGATATTTTTTCAGGTATGCGGCCAACTGGGTTCATTGAGAATCACGATCGGGCCCCAGAGCGGCAGAAATCTAGGGGTCCGATCATTGAGGAACTAGACTCTGATCATGAAGAAGAGGAGGCTAATAATGAGAAAAAGGAAAATCCGCGGAAACGTGTTGGGCCAAGCAATGATCCTTTTGTTGAGCACCCTGATGATGTAGTTGAAGAGAAGAAGAGCAAGCATTTGCAATCTAGGGACAACTACAGTCAGTTTGGTGCTATGCAATCACAGCCTCAAGCTCATAGCTACACGTTTCAGAGCTCCAGTGTCACTTATGGTGGTGGTAATGGTGCATACTATACATCTTCCAAGACAAGAAGGATGGGGAGTGATGGGGTTGCTTTTGAAGAAAGCAAGGAAGCTGATACTTCTTCTGGGAAAGCGACACACAGGGTATCTAAGGGGCTCCACAATAAGGGCCATTCAATTACTAGGAAGCTCAAAGAGGGTAAGGTAGATACAATGCAGACTTTGCATAACCTTAATGAAGATGAGCTTTCCGGTTTTGAACATGCATGGAAGGGAGCTGCTCAAAAACATATGCCTGGATTGAATGATCAGTTTGCTAGTTATGAAAGTCTAGGAGCTGGCGGCAGCAGACATGGGCAGGCCGGTCAGGGAGGTTGGGCACTTCCTAGCAACGATCTTCTACAGAACTCTGGAAATATCATAGCAGATGCCCAAGGCAAAGCAGTACCTTATCAGTCGCAGCAGTCTGGTAATATGAGAGAAGGTGGTACTAATTCTTCCCGTGGGAGAGCAAAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 map05202
Pfam Domains
Protein Families
Mlf1IP

Protein Analysis

364

Amino Acids

38.93

Weight (kDa)

6.19

Isoelectric Point (pI)

49.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlf1IP PF10248 133 - 283 3.3e-20 Myelodysplasia-myeloid leukemia factor 1-interacting protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015615)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g00720
malus_domestica MD04G1079300.v1.1
prunus_persica Prupe.5G079800_v2.0.a1
pyrus_communis pycom04g07110
rosa_chinensis RchiOBHm_Chr7g0201491
rosa_laevigata RLG00000003669
rosa_multiflora Rmu_co8397869.1_g000001 Rmu_sc0002046.1_g000005 Rmu_sc0017656.1_g000004
rosa_roxburghii Rroxscaffold_3G00255260
rosa_rugosa Rorug07G0064300
rosa_samantha Rh7AG190900 Rh7BG193200 Rh7CG202300 Rh7DG195900
rosa_wichuraiana Rw7G016740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 343
AccII CGCG 1 cut(s) 430
AciI CCGC 7 cut(s) 85, 246, 301, 343, 428, 430, 915
AclWI GGATC 4 cut(s) 50, 119, 132, 449
AcoI YGGCCR 1 cut(s) 302
AfaI GTAC 2 cut(s) 1020, 1063
AfiI CCNNNNNNNGG 8 cut(s) 65, 161, 191, 215, 239, 263, 342, 739
AflIII ACRYGT 2 cut(s) 436, 573
AgsI TTSAA 6 cut(s) 52, 488, 671, 750, 833, 877
AjnI CCWGG 6 cut(s) 25, 208, 232, 256, 268, 868
AluBI AGCT 8 cut(s) 563, 570, 585, 686, 763, 820, 851, 911
AluI AGCT 8 cut(s) 563, 570, 585, 686, 763, 820, 851, 911
Alw21I GWGCWC 2 cut(s) 471, 587
AlwI GGATC 4 cut(s) 50, 119, 132, 449
Ama87I CYCGRG 1 cut(s) 175
AoxI GGCC 5 cut(s) 302, 333, 443, 742, 933
ApaI GGGCCC 1 cut(s) 337
ApeKI GCWGC 3 cut(s) 851, 918, 1033
AspS9I GGNCC 8 cut(s) 23, 38, 266, 333, 334, 359, 443, 742
AsuC2I CCSGG 1 cut(s) 186
AvaI CYCGRG 1 cut(s) 175
AvaII GGWCC 4 cut(s) 23, 38, 266, 359
BaeGI GKGCMC 2 cut(s) 337, 955
BamHI GGATCC 1 cut(s) 124
BanII GRGCYC 3 cut(s) 337, 587, 732
BarI GAAGNNNNNNTAC 2 cut(s) 1054, 1086
BbsI GAAGAC 1 cut(s) 103
Bbv12I GWGCWC 2 cut(s) 471, 587
BbvI GCAGC 3 cut(s) 838, 930, 1045
BccI CCATC 2 cut(s) 640, 650
BciT130I CCWGG 6 cut(s) 27, 210, 234, 258, 270, 870
BclI TGATCA 2 cut(s) 385, 880
BcnI CCSGG 1 cut(s) 186
BfaI CTAG 7 cut(s) 354, 377, 516, 756, 891, 905, 960
BfmI CTRYAG 2 cut(s) 526, 974
BisI GCNGC 7 cut(s) 86, 302, 344, 852, 916, 919, 1034
BlsI GCNGC 7 cut(s) 87, 303, 345, 853, 917, 920, 1035
Bme1390I CCNGG 7 cut(s) 27, 186, 210, 234, 258, 270, 870
Bme18I GGWCC 4 cut(s) 23, 38, 266, 359
BmeT110I CYCGRG 1 cut(s) 175
BmgT120I GGNCC 8 cut(s) 23, 38, 266, 333, 334, 359, 443, 742
BmiI GGNNCC 7 cut(s) 39, 126, 267, 335, 336, 360, 731
BmrFI CCNGG 7 cut(s) 27, 186, 210, 234, 258, 270, 870
BmrI ACTGGG 1 cut(s) 318
BmsI GCATC 1 cut(s) 991
BmuI ACTGGG 1 cut(s) 318
BpiI GAAGAC 1 cut(s) 103
BpmI CTGGAG 1 cut(s) 571
BpuEI CTTGAG 1 cut(s) 543
BpuMI CCSGG 1 cut(s) 186
BsaJI CCNNGG 3 cut(s) 428, 1006, 1075
BsaWI WCCGGW 1 cut(s) 824
Bsc4I CCNNNNNNNGG 8 cut(s) 65, 161, 191, 215, 239, 263, 342, 739
Bse118I RCCGGY 1 cut(s) 935
Bse1I ACTGG 2 cut(s) 313, 588
Bse3DI GCAATG 1 cut(s) 457
BseBI CCWGG 6 cut(s) 27, 210, 234, 258, 270, 870
BseDI CCNNGG 3 cut(s) 428, 1006, 1075
BseGI GGATG 1 cut(s) 651
BseLI CCNNNNNNNGG 8 cut(s) 65, 161, 191, 215, 239, 263, 342, 739
BseMI GCAATG 1 cut(s) 457
BseNI ACTGG 2 cut(s) 313, 588
BseRI GAGGAG 1 cut(s) 413
BseSI GKGCMC 2 cut(s) 337, 955
BseXI GCAGC 3 cut(s) 838, 930, 1045
Bsh1236I CGCG 1 cut(s) 430
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 5 cut(s) 304, 335, 445, 744, 935
BsiEI CGRYCG 1 cut(s) 331
BsiHKAI GWGCWC 2 cut(s) 471, 587
BsiHKCI CYCGRG 1 cut(s) 175
BsiSI CCGG 3 cut(s) 185, 825, 936
BslFI GGGAC 1 cut(s) 533
BslI CCNNNNNNNGG 8 cut(s) 65, 161, 191, 215, 239, 263, 342, 739
BsmFI GGGAC 1 cut(s) 533
BsnI GGCC 5 cut(s) 304, 335, 445, 744, 935
BsoBI CYCGRG 1 cut(s) 175
Bsp120I GGGCCC 1 cut(s) 333
Bsp1286I GDGCHC 5 cut(s) 337, 471, 587, 732, 955
Bsp143I GATC 8 cut(s) 55, 124, 328, 363, 385, 454, 880, 967
BspACI CCGC 7 cut(s) 85, 246, 301, 343, 428, 430, 915
BspANI GGCC 5 cut(s) 304, 335, 445, 744, 935
BspFNI CGCG 1 cut(s) 430
BspHI TCATGA 1 cut(s) 388
BspLI GGNNCC 7 cut(s) 39, 126, 267, 335, 336, 360, 731
BspPI GGATC 4 cut(s) 50, 119, 132, 449
BspQI GCTCTTC 1 cut(s) 491
BsrBI CCGCTC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 457
BsrFI RCCGGY 1 cut(s) 935
BsrI ACTGG 2 cut(s) 313, 588
BssAI RCCGGY 1 cut(s) 935
BssECI CCNNGG 3 cut(s) 428, 1006, 1075
BssMI GATC 8 cut(s) 55, 124, 328, 363, 385, 454, 880, 967
BssT1I CCWWGG 1 cut(s) 1006
Bst2UI CCWGG 6 cut(s) 27, 210, 234, 258, 270, 870
Bst4CI ACNGT 1 cut(s) 530
Bst6I CTCTTC 4 cut(s) 7, 390, 483, 491
BstAPI GCANNNNNTGC 1 cut(s) 796
BstC8I GCNNGC 3 cut(s) 503, 913, 933
BstDEI CTNAG 1 cut(s) 723
BstDSI CCRYGG 2 cut(s) 428, 1075
BstF5I GGATG 1 cut(s) 651
BstFNI CGCG 1 cut(s) 430
BstKTI GATC 8 cut(s) 58, 127, 331, 366, 388, 457, 883, 970
BstMBI GATC 8 cut(s) 55, 124, 328, 363, 385, 454, 880, 967
BstMCI CGRYCG 1 cut(s) 331
BstMWI GCNNNNNNNGC 2 cut(s) 553, 796
BstNI CCWGG 6 cut(s) 27, 210, 234, 258, 270, 870
BstNSI RCATGY 2 cut(s) 205, 839
BstSCI CCNGG 7 cut(s) 25, 184, 208, 232, 256, 268, 868
BstSFI CTRYAG 2 cut(s) 526, 974
BstSLI GKGCMC 2 cut(s) 337, 955
BstUI CGCG 1 cut(s) 430
BstV1I GCAGC 3 cut(s) 838, 930, 1045
BstV2I GAAGAC 1 cut(s) 103
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BsuRI GGCC 5 cut(s) 304, 335, 445, 744, 935
BtgI CCRYGG 2 cut(s) 428, 1075
BtsCI GGATG 1 cut(s) 651
BtsIMutI CAGTG 1 cut(s) 595
Cac8I GCNNGC 3 cut(s) 503, 913, 933
CciI TCATGA 1 cut(s) 388
Cfr10I RCCGGY 1 cut(s) 935
Cfr13I GGNCC 8 cut(s) 23, 38, 266, 333, 334, 359, 443, 742
Cfr42I CCGCGG 1 cut(s) 431
CsiI ACCWGGT 1 cut(s) 25
Csp6I GTAC 2 cut(s) 1019, 1062
CviAII CATG 5 cut(s) 202, 389, 836, 840, 926
CviQI GTAC 2 cut(s) 1019, 1062
DdeI CTNAG 1 cut(s) 723
DpnI GATC 8 cut(s) 57, 126, 330, 365, 387, 456, 882, 969
DpnII GATC 8 cut(s) 55, 124, 328, 363, 385, 454, 880, 967
EaeI YGGCCR 1 cut(s) 302
Eam1104I CTCTTC 4 cut(s) 7, 390, 483, 491
EarI CTCTTC 4 cut(s) 7, 390, 483, 491
Ecl136II GAGCTC 1 cut(s) 585
Eco130I CCWWGG 1 cut(s) 1006
Eco24I GRGCYC 3 cut(s) 337, 587, 732
Eco47I GGWCC 4 cut(s) 23, 38, 266, 359
Eco53kI GAGCTC 1 cut(s) 585
Eco88I CYCGRG 1 cut(s) 175
EcoICRI GAGCTC 1 cut(s) 585
EcoO109I RGGNCCY 4 cut(s) 23, 38, 266, 334
EcoRII CCWGG 6 cut(s) 25, 208, 232, 256, 268, 868
EcoT14I CCWWGG 1 cut(s) 1006
EcoT22I ATGCAT 1 cut(s) 841
EcoT38I GRGCYC 3 cut(s) 337, 587, 732
ErhI CCWWGG 1 cut(s) 1006
FaeI CATG 5 cut(s) 205, 392, 839, 843, 929
FalI AAGNNNNNCTT 2 cut(s) 804, 836
FaqI GGGAC 1 cut(s) 533
FatI CATG 5 cut(s) 201, 388, 835, 839, 925
FauNDI CATATG 1 cut(s) 864
FbaI TGATCA 2 cut(s) 385, 880
Fnu4HI GCNGC 7 cut(s) 86, 302, 344, 852, 916, 919, 1034
FokI GGATG 1 cut(s) 658
FriOI GRGCYC 3 cut(s) 337, 587, 732
Fsp4HI GCNGC 7 cut(s) 86, 302, 344, 852, 916, 919, 1034
FspBI CTAG 7 cut(s) 354, 377, 516, 756, 891, 905, 960
GluI GCNGC 7 cut(s) 86, 302, 344, 852, 916, 919, 1034
GsuI CTGGAG 1 cut(s) 571
HaeIII GGCC 5 cut(s) 304, 335, 445, 744, 935
HapII CCGG 3 cut(s) 185, 825, 936
Hin1II CATG 5 cut(s) 205, 392, 839, 843, 929
HinfI GANTC 3 cut(s) 173, 322, 380
HpaII CCGG 3 cut(s) 185, 825, 936
Hpy188I TCNGA 4 cut(s) 116, 363, 385, 582
Hpy188III TCNNGA 3 cut(s) 326, 389, 984
HpyAV CCTTC 3 cut(s) 636, 838, 1049
HpyCH4III ACNGT 1 cut(s) 530
HpyCH4IV ACGT 3 cut(s) 108, 436, 575
HpyCH4V TGCA 7 cut(s) 4, 511, 547, 617, 790, 799, 839
HpyF10VI GCNNNNNNNGC 2 cut(s) 553, 796
HpyF3I CTNAG 1 cut(s) 723
HpySE526I ACGT 3 cut(s) 108, 436, 575
Hsp92II CATG 5 cut(s) 205, 392, 839, 843, 929
Ksp22I TGATCA 2 cut(s) 385, 880
KspI CCGCGG 1 cut(s) 431
Kzo9I GATC 8 cut(s) 55, 124, 328, 363, 385, 454, 880, 967
LguI GCTCTTC 1 cut(s) 491
LmnI GCTCC 4 cut(s) 590, 735, 848, 908
Lsp1109I GCAGC 3 cut(s) 838, 930, 1045
LweI GCATC 1 cut(s) 991
MabI ACCWGGT 1 cut(s) 25
MaeI CTAG 7 cut(s) 354, 377, 516, 756, 891, 905, 960
MaeII ACGT 3 cut(s) 108, 436, 575
MaeIII GTNAC 1 cut(s) 592
MalI GATC 8 cut(s) 57, 126, 330, 365, 387, 456, 882, 969
MbiI CCGCTC 1 cut(s) 343
MboI GATC 8 cut(s) 55, 124, 328, 363, 385, 454, 880, 967
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 5 cut(s) 337, 471, 587, 732, 955
MluCI AATT 2 cut(s) 750, 1066
MlyI GAGTC 2 cut(s) 167, 374
Mph1103I ATGCAT 1 cut(s) 841
MseI TTAA 2 cut(s) 807, 1093
MslI CAYNNNNRTG 1 cut(s) 474
MspA1I CMGCKG 1 cut(s) 430
MspI CCGG 3 cut(s) 185, 825, 936
MspR9I CCNGG 7 cut(s) 27, 186, 210, 234, 258, 270, 870
MvaI CCWGG 6 cut(s) 27, 210, 234, 258, 270, 870
MvnI CGCG 1 cut(s) 430
MwoI GCNNNNNNNGC 2 cut(s) 553, 796
NciI CCSGG 1 cut(s) 186
NdeI CATATG 1 cut(s) 864
NdeII GATC 8 cut(s) 55, 124, 328, 363, 385, 454, 880, 967
NlaIII CATG 5 cut(s) 205, 392, 839, 843, 929
NlaIV GGNNCC 7 cut(s) 39, 126, 267, 335, 336, 360, 731
NmuCI GTSAC 1 cut(s) 592
NsiI ATGCAT 1 cut(s) 841
NspI RCATGY 2 cut(s) 205, 839
PagI TCATGA 1 cut(s) 388
PciSI GCTCTTC 1 cut(s) 491
PfeI GAWTC 1 cut(s) 322
PfoI TCCNGGA 3 cut(s) 208, 232, 256
PkrI GCNGC 7 cut(s) 87, 303, 345, 853, 917, 920, 1035
Ple19I CGATCG 1 cut(s) 331
PleI GAGTC 2 cut(s) 167, 374
PpsI GAGTC 2 cut(s) 167, 374
PpuMI RGGWCCY 3 cut(s) 23, 38, 266
Psp124BI GAGCTC 1 cut(s) 587
Psp5II RGGWCCY 3 cut(s) 23, 38, 266
Psp6I CCWGG 6 cut(s) 25, 208, 232, 256, 268, 868
PspGI CCWGG 6 cut(s) 25, 208, 232, 256, 268, 868
PspN4I GGNNCC 7 cut(s) 39, 126, 267, 335, 336, 360, 731
PspOMI GGGCCC 1 cut(s) 333
PspPI GGNCC 8 cut(s) 23, 38, 266, 333, 334, 359, 443, 742
PspPPI RGGWCCY 3 cut(s) 23, 38, 266
PsuI RGATCY 1 cut(s) 124
PvuI CGATCG 1 cut(s) 331
RsaI GTAC 2 cut(s) 1020, 1063
RsaNI GTAC 2 cut(s) 1019, 1062
RseI CAYNNNNRTG 1 cut(s) 474
SacI GAGCTC 1 cut(s) 587
SacII CCGCGG 1 cut(s) 431
SapI GCTCTTC 1 cut(s) 491
SaqAI TTAA 2 cut(s) 807, 1093
SatI GCNGC 7 cut(s) 86, 302, 344, 852, 916, 919, 1034
Sau3AI GATC 8 cut(s) 55, 124, 328, 363, 385, 454, 880, 967
Sau96I GGNCC 8 cut(s) 23, 38, 266, 333, 334, 359, 443, 742
SchI GAGTC 2 cut(s) 167, 374
ScrFI CCNGG 7 cut(s) 27, 186, 210, 234, 258, 270, 870
SduI GDGCHC 5 cut(s) 337, 471, 587, 732, 955
SexAI ACCWGGT 1 cut(s) 25
SfaNI GCATC 1 cut(s) 991
SfcI CTRYAG 2 cut(s) 526, 974
Sfr303I CCGCGG 1 cut(s) 431
SgrBI CCGCGG 1 cut(s) 431
SinI GGWCC 4 cut(s) 23, 38, 266, 359
SmiMI CAYNNNNRTG 1 cut(s) 474
SmlI CTYRAG 1 cut(s) 558
SmoI CTYRAG 1 cut(s) 558
Sse9I AATT 2 cut(s) 750, 1066
SsiI CCGC 7 cut(s) 85, 246, 301, 343, 428, 430, 915
SspMI CTAG 7 cut(s) 354, 377, 516, 756, 891, 905, 960
SstI GAGCTC 1 cut(s) 587
StyD4I CCNGG 7 cut(s) 25, 184, 208, 232, 256, 268, 868
StyI CCWWGG 1 cut(s) 1006
TaaI ACNGT 1 cut(s) 530
TaiI ACGT 3 cut(s) 111, 439, 578
TasI AATT 2 cut(s) 750, 1066
TauI GCSGC 4 cut(s) 88, 304, 346, 918
TfiI GAWTC 1 cut(s) 322
Tru1I TTAA 2 cut(s) 807, 1093
Tru9I TTAA 2 cut(s) 807, 1093
TscAI CASTG 1 cut(s) 595
TseFI GTSAC 1 cut(s) 592
TseI GCWGC 3 cut(s) 851, 918, 1033
Tsp45I GTSAC 1 cut(s) 592
TspDTI ATGAA 5 cut(s) 290, 304, 405, 825, 912
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 1 cut(s) 595
VpaK11BI GGWCC 4 cut(s) 23, 38, 266, 359
XceI RCATGY 2 cut(s) 205, 839
XspI CTAG 7 cut(s) 354, 377, 516, 756, 891, 905, 960
Zsp2I ATGCAT 1 cut(s) 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.