MD04G1086100.v1.1

Oxysterol-binding protein-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001060
Physical Location & Seq
Forward (+)
12529799 .. 12538245
8447 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1086100.v1.1.491

Sequence Viewer

Length: 2223 bp
ATGCGGGTCAAAGAGATGCACCCACTCTGCTGCATCACGCTCGAGAGCGCGGGTCTCGGCGACCAATCGCCGGAGATCTCGCTGTCCCGGACCCGGAGCCTCCCGGCGAGTCTGGCCGCGGCGCAGGTCGGATCTGACTCTAATACCGCCGGCCGAGCATCGGGATCTGAGGCCACCGTTGCCGGAGTCCTCCACAAGTGGACCAATTACAGCAAGGGTTGGAGATCCAGGTGGTTCCTTCTCCGAAACGGCATCCTCTCCTACTCCAAGATTCGACGACCCGAAACGCTAAGCCTGTTAACCCCAAACGACGACGACGTTCGACTCATCGGCGAGATCTCCGCTCACCGCCTTTCCCGGATGGACAGCGAGACCGGAAGCCGGCGGAGCAACAAACCCCCGAAAACGGTTGGAATCGTCCACCTCAAGATCTCATCCTTCCGAGAAAGCAAGTCCGACGATCGGAAATTTTACATATTTACTGCTACAAAGACCCTTCATTTAAGGACTAATTCAAAGAGCGATCGGGCGGCTTGGCTACAAGCTTTGGTATCAATTCGGACTCTATTTCCTCATAGATCCTTGAATGATAGTCTCTCCCTTGTACCAACTGATTTATCTATATCGACCAAAAGACTGAAAAAGCGCCTGCTTGAAGAGGGAACCGGTGAGAGCATTGTACAGGACTGCGAGCAGATAATGCTCTCGGAGTTCTCTCAATTACAAGGACGGCTTAAGGTTATATGTGAAGAACGATCAAATTTACTGGACACATTAAGGCAGTTCGAGGCAGCTAATTATGAAGCTGAAGCCTCTGGAATTCATGACGGTGAATACCAATTGACAAAGCACGAGTTTTCAAGTTTGGGACCGGGAAAATATAGTGAGTGCAGTACAACTGAATCATCTGACGATATTGAGAAACAAGAGCTTGAGGAAGTGTCAGATGAGGATGAAATGCCATTTCATGACACAAAAGAGTATTTCACTGAACCTAGCATAACTTGTGGGTCTATGAAAGGAGTGGTAAATAACACTAATAAGCAAAGTGAAGCTCAAAGTCAAACTAATAATGGTGAGGAGACTCAAACTGAGAAGGAAGTTCAGGATTATAATTACCCACACGTTGAAAGGAGAAAAGAGCTTCCCACTCCAGTTGAGAAGGAGAAGGGGGTCAGCCTTTGGTCTATGATTAAAGACAATGTGGGAAAGGATCTCACTCGAGTATGCCTTCCTGTGTACTTTAATGAGCCAATATCATCTCTTCAGAAGTGTTGTGAGGATTTGGAGTACTCGTATCTTTTGGACCGAGCATATGAGTATGGGAAAATGGGAAACAGTCTCCAAAGGGTTCTAAATGTTGCTGCATTTGCGGTTTCTGGATATGCGTCATCTGTAGGGCGGCACTGCAAGCCATTCAATCCCTTGTTAGGGGAAACTTATGAAGCTGACTATCCTGATGAAGGAATTCGCTTCTTCTCCGAAAAGGTTAGTCACCATCCAACACTCATTGCCTGTCATTGTGAAGGTAGAGGGTGGAAGTTCTGGGCTGACAGCAATATCCACACAAAATTTTGGGGACGATCAATTCAGCTTGACCCCGTTGGAGTTCTTAGCTTGCAGTTTGATGATGGTGAACTTTTCCAGTGGAGCAAGGTCACAACAAGCATATACAATCTAATTCTTGGTAAAGTATATTGTGATCACCATGGTACAATGCATATACGTGGTAATCGTCAGCATTCATGCAGACTGAAGTTCAAAGAGCAATCGATTCTTGACCGAAATCCTCACCAGGTCCATGGATTTGTGGAAGATGTCACGGGGAAAAAGGTTGCTACATTATTTGGGAAGTGGGATGAAAGCATGCATTATGTTAATGGTGATGGGAGTCGCAAGTTAAATCCTTCAGATGCTTCATTGTTGTGGAAAAGTAGTAAACCTCCTAATGTCACACGATACAACTTAACATCATTCGCAATGACACTGAATGAGCTAACACCTGGACTGCAGAAGCTCCCACCCACCGATTCCAGGCTCAGGCCGGACCAACGGCATCTAGAGAATGGAGAGTACGAAAAGGCAAATGCAGAGAAACAGCGATTGGAAAAAAGGCAAAGAATGGTATTTTGGTTTCTTTTCGTATCTTTTGGTCACACACACACACACGCACACACAGAGACACACACACACACAGACACCCATGCCAGCCTCATCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oxysterol_BP PH_11

Protein Analysis

741

Amino Acids

84.0

Weight (kDa)

6.68

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 61 - 185 1.4e-08 PH domain
PH_11 PF15413 66 - 183 1.4e-08 Pleckstrin homology domain
Oxysterol_BP PF01237 393 - 708 4.8e-118 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1113
Acc36I ACCTGC 1 cut(s) 115
AccBSI CCGCTC 1 cut(s) 344
AccII CGCG 2 cut(s) 50, 119
AclWI GGATC 5 cut(s) 139, 172, 219, 573, 1221
AcoI YGGCCR 2 cut(s) 114, 151
AcsI RAATTY 5 cut(s) 467, 760, 819, 1467, 1571
AcuI CTGAAG 4 cut(s) 828, 1250, 1775, 1893
AfaI GTAC 7 cut(s) 606, 681, 895, 1241, 1292, 1714, 2075
AflII CTTAAG 1 cut(s) 734
AflIII ACRYGT 1 cut(s) 1123
AgeI ACCGGT 1 cut(s) 665
AgsI TTSAA 7 cut(s) 516, 586, 656, 861, 1130, 1420, 1762
AjnI CCWGG 4 cut(s) 227, 1794, 2002, 2033
AjuI GAANNNNNNNTTGG 4 cut(s) 2087, 2113, 2119, 2145
Alw26I GTCTC 6 cut(s) 59, 365, 599, 1076, 1346, 2174
AlwI GGATC 5 cut(s) 139, 172, 219, 573, 1221
Ama87I CYCGRG 2 cut(s) 41, 1221
AoxI GGCC 4 cut(s) 114, 151, 171, 2042
ApeKI GCWGC 3 cut(s) 30, 791, 1364
ApoI RAATTY 5 cut(s) 467, 760, 819, 1467, 1571
AsiGI ACCGGT 1 cut(s) 665
Asp700I GAANNNNTTC 1 cut(s) 1467
AspLEI GCGC 3 cut(s) 50, 124, 648
AspS9I GGNCC 6 cut(s) 90, 201, 869, 1306, 1798, 2047
AsuC2I CCSGG 5 cut(s) 88, 94, 104, 358, 873
AsuHPI GGTGA 9 cut(s) 338, 680, 842, 1088, 1487, 1646, 1697, 1784, 1895
AvaI CYCGRG 2 cut(s) 41, 1221
AvaII GGWCC 6 cut(s) 90, 201, 869, 1306, 1798, 2047
BauI CACGAG 1 cut(s) 851
BbvI GCAGC 3 cut(s) 17, 803, 1351
BccI CCATC 4 cut(s) 355, 1506, 1625, 1880
BceAI ACGGC 3 cut(s) 265, 746, 2069
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BciT130I CCWGG 4 cut(s) 229, 1796, 2004, 2035
BclI TGATCA 1 cut(s) 1702
BcnI CCSGG 5 cut(s) 88, 94, 104, 358, 873
BcoDI GTCTC 6 cut(s) 59, 365, 599, 1076, 1346, 2174
BfaI CTAG 2 cut(s) 996, 2060
BfmI CTRYAG 2 cut(s) 1395, 2009
BfoI RGCGCY 1 cut(s) 649
BfrI CTTAAG 1 cut(s) 734
BfuAI ACCTGC 1 cut(s) 115
BglII AGATCT 3 cut(s) 75, 336, 429
BisI GCNGC 7 cut(s) 31, 117, 120, 531, 792, 1365, 1403
BlpI GCTNAGC 1 cut(s) 290
BlsI GCNGC 7 cut(s) 32, 118, 121, 532, 793, 1366, 1404
BmcAI AGTACT 1 cut(s) 1292
Bme1390I CCNGG 9 cut(s) 88, 94, 104, 229, 358, 873, 1796, 2004, 2035
Bme18I GGWCC 6 cut(s) 90, 201, 869, 1306, 1798, 2047
BmeT110I CYCGRG 2 cut(s) 41, 1221
BmgT120I GGNCC 6 cut(s) 90, 201, 869, 1306, 1798, 2047
BmiI GGNNCC 5 cut(s) 92, 98, 236, 664, 870
BmrFI CCNGG 9 cut(s) 88, 94, 104, 229, 358, 873, 1796, 2004, 2035
BmsI GCATC 6 cut(s) 6, 42, 167, 261, 1903, 2065
BpmI CTGGAG 1 cut(s) 1137
Bpu10I CCTNAGC 1 cut(s) 2039
Bpu1102I GCTNAGC 1 cut(s) 290
BpuEI CTTGAG 2 cut(s) 410, 953
BpuMI CCSGG 5 cut(s) 88, 94, 104, 358, 873
Bsa29I ATCGAT 1 cut(s) 1772
BsaAI YACGTR 1 cut(s) 1727
BsaI GGTCTC 2 cut(s) 59, 365
BsaJI CCNNGG 3 cut(s) 117, 1708, 1801
BsaWI WCCGGW 2 cut(s) 374, 665
BsaXI ACNNNNNCTCC 2 cut(s) 1280, 1310
Bse118I RCCGGY 3 cut(s) 149, 381, 665
Bse1I ACTGG 3 cut(s) 771, 1154, 1645
Bse3DI GCAATG 2 cut(s) 1509, 1986
BseBI CCWGG 4 cut(s) 229, 1796, 2004, 2035
BseCI ATCGAT 1 cut(s) 1772
BseDI CCNNGG 3 cut(s) 117, 1708, 1801
BseGI GGATG 6 cut(s) 252, 366, 434, 958, 1498, 1864
BseMI GCAATG 2 cut(s) 1509, 1986
BseMII CTCAG 3 cut(s) 159, 1083, 2053
BseNI ACTGG 3 cut(s) 771, 1154, 1645
BseRI GAGGAG 1 cut(s) 1094
BseX3I CGGCCG 1 cut(s) 151
BseXI GCAGC 3 cut(s) 17, 803, 1351
BsgI GTGCAG 1 cut(s) 910
Bsh1236I CGCG 2 cut(s) 50, 119
Bsh1285I CGRYCG 3 cut(s) 154, 463, 526
BshFI GGCC 4 cut(s) 116, 153, 173, 2044
BshTI ACCGGT 1 cut(s) 665
BshVI ATCGAT 1 cut(s) 1772
BsiEI CGRYCG 3 cut(s) 154, 463, 526
BsiHKCI CYCGRG 2 cut(s) 41, 1221
BslFI GGGAC 3 cut(s) 70, 882, 1593
BsmAI GTCTC 6 cut(s) 59, 365, 599, 1076, 1346, 2174
BsmFI GGGAC 3 cut(s) 70, 882, 1593
BsmI GAATGC 1 cut(s) 1741
BsnI GGCC 4 cut(s) 116, 153, 173, 2044
Bso31I GGTCTC 2 cut(s) 59, 365
BsoBI CYCGRG 2 cut(s) 41, 1221
Bsp1407I TGTACA 1 cut(s) 679
Bsp1720I GCTNAGC 1 cut(s) 290
Bsp19I CCATGG 2 cut(s) 1708, 1801
BspANI GGCC 4 cut(s) 116, 153, 173, 2044
BspCNI CTCAG 3 cut(s) 160, 1084, 2052
BspDI ATCGAT 1 cut(s) 1772
BspFNI CGCG 2 cut(s) 50, 119
BspHI TCATGA 2 cut(s) 823, 967
BspLI GGNNCC 5 cut(s) 92, 98, 236, 664, 870
BspMAI CTGCAG 1 cut(s) 2013
BspMI ACCTGC 1 cut(s) 115
BspPI GGATC 5 cut(s) 139, 172, 219, 573, 1221
BspTI CTTAAG 1 cut(s) 734
BspTNI GGTCTC 2 cut(s) 59, 365
BsrBI CCGCTC 1 cut(s) 344
BsrDI GCAATG 2 cut(s) 1509, 1986
BsrFI RCCGGY 3 cut(s) 149, 381, 665
BsrGI TGTACA 1 cut(s) 679
BsrI ACTGG 3 cut(s) 771, 1154, 1645
BssAI RCCGGY 3 cut(s) 149, 381, 665
BssECI CCNNGG 3 cut(s) 117, 1708, 1801
BssSI CACGAG 1 cut(s) 851
BssT1I CCWWGG 2 cut(s) 1708, 1801
Bst2BI CACGAG 1 cut(s) 851
Bst2UI CCWGG 4 cut(s) 229, 1796, 2004, 2035
Bst4CI ACNGT 4 cut(s) 178, 409, 830, 1340
Bst6I CTCTTC 2 cut(s) 651, 1269
BstAFI CTTAAG 1 cut(s) 734
BstAPI GCANNNNNTGC 1 cut(s) 700
BstAUI TGTACA 1 cut(s) 679
BstBAI YACGTR 1 cut(s) 1727
BstC8I GCNNGC 8 cut(s) 151, 383, 650, 692, 1412, 1619, 1868, 2209
BstDEI CTNAG 5 cut(s) 168, 290, 1092, 1613, 2039
BstDSI CCRYGG 3 cut(s) 117, 1708, 1801
BstENI CCTNNNNNAGG 1 cut(s) 1461
BstF5I GGATG 6 cut(s) 252, 366, 434, 958, 1498, 1864
BstFNI CGCG 2 cut(s) 50, 119
BstH2I RGCGCY 1 cut(s) 649
BstHHI GCGC 3 cut(s) 50, 124, 648
BstMAI GTCTC 6 cut(s) 59, 365, 599, 1076, 1346, 2174
BstMCI CGRYCG 3 cut(s) 154, 463, 526
BstMWI GCNNNNNNNGC 8 cut(s) 113, 155, 179, 387, 700, 1370, 1411, 2217
BstNI CCWGG 4 cut(s) 229, 1796, 2004, 2035
BstNSI RCATGY 1 cut(s) 1870
BstSCI CCNGG 9 cut(s) 86, 92, 102, 227, 356, 871, 1794, 2002, 2033
BstSFI CTRYAG 2 cut(s) 1395, 2009
BstUI CGCG 2 cut(s) 50, 119
BstV1I GCAGC 3 cut(s) 17, 803, 1351
BstX2I RGATCY 8 cut(s) 75, 131, 164, 224, 336, 429, 578, 1213
BstXI CCANNNNNNTGG 1 cut(s) 1802
BstYI RGATCY 8 cut(s) 75, 131, 164, 224, 336, 429, 578, 1213
BstZI CGGCCG 1 cut(s) 151
Bsu15I ATCGAT 1 cut(s) 1772
BsuRI GGCC 4 cut(s) 116, 153, 173, 2044
BsuTUI ATCGAT 1 cut(s) 1772
BtgI CCRYGG 3 cut(s) 117, 1708, 1801
BtsCI GGATG 6 cut(s) 252, 366, 434, 958, 1498, 1864
BtsI GCAGTG 1 cut(s) 1405
BtsIMutI CAGTG 4 cut(s) 987, 1405, 1652, 1985
BveI ACCTGC 1 cut(s) 115
Cac8I GCNNGC 8 cut(s) 151, 383, 650, 692, 1412, 1619, 1868, 2209
CciI TCATGA 2 cut(s) 823, 967
CfoI GCGC 3 cut(s) 50, 124, 648
Cfr10I RCCGGY 3 cut(s) 149, 381, 665
Cfr13I GGNCC 6 cut(s) 90, 201, 869, 1306, 1798, 2047
Cfr42I CCGCGG 1 cut(s) 120
ClaI ATCGAT 1 cut(s) 1772
CseI GACGC 1 cut(s) 1377
CsiI ACCWGGT 1 cut(s) 1794
Csp6I GTAC 7 cut(s) 605, 680, 894, 1240, 1291, 1713, 2074
CspAI ACCGGT 1 cut(s) 665
CspCI CAANNNNNGTGG 2 cut(s) 1138, 1173
CviAII CATG 7 cut(s) 824, 968, 1709, 1746, 1802, 1867, 2204
CviQI GTAC 7 cut(s) 605, 680, 894, 1240, 1291, 1713, 2074
DdeI CTNAG 5 cut(s) 168, 290, 1092, 1613, 2039
EaeI YGGCCR 2 cut(s) 114, 151
EagI CGGCCG 1 cut(s) 151
Eam1104I CTCTTC 2 cut(s) 651, 1269
EarI CTCTTC 2 cut(s) 651, 1269
EciI GGCGGA 1 cut(s) 400
EclXI CGGCCG 1 cut(s) 151
Eco130I CCWWGG 2 cut(s) 1708, 1801
Eco31I GGTCTC 2 cut(s) 59, 365
Eco47I GGWCC 6 cut(s) 90, 201, 869, 1306, 1798, 2047
Eco52I CGGCCG 1 cut(s) 151
Eco57I CTGAAG 4 cut(s) 828, 1250, 1775, 1893
Eco88I CYCGRG 2 cut(s) 41, 1221
EcoNI CCTNNNNNAGG 1 cut(s) 1461
EcoRI GAATTC 2 cut(s) 819, 1467
EcoRII CCWGG 4 cut(s) 227, 1794, 2002, 2033
EcoT14I CCWWGG 2 cut(s) 1708, 1801
EcoT22I ATGCAT 2 cut(s) 1722, 1872
ErhI CCWWGG 2 cut(s) 1708, 1801
FaeI CATG 7 cut(s) 827, 971, 1712, 1749, 1805, 1870, 2207
FalI AAGNNNNNCTT 2 cut(s) 717, 749
FaqI GGGAC 3 cut(s) 70, 882, 1593
FatI CATG 7 cut(s) 823, 967, 1708, 1745, 1801, 1866, 2203
FauI CCCGC 1 cut(s) 43
FauNDI CATATG 1 cut(s) 1315
FbaI TGATCA 1 cut(s) 1702
Fnu4HI GCNGC 7 cut(s) 31, 117, 120, 531, 792, 1365, 1403
FokI GGATG 6 cut(s) 239, 373, 421, 965, 1485, 1871
Fsp4HI GCNGC 7 cut(s) 31, 117, 120, 531, 792, 1365, 1403
FspBI CTAG 2 cut(s) 996, 2060
GlaI GCGC 3 cut(s) 49, 123, 647
GluI GCNGC 7 cut(s) 31, 117, 120, 531, 792, 1365, 1403
GsuI CTGGAG 1 cut(s) 1137
HaeII RGCGCY 1 cut(s) 649
HaeIII GGCC 4 cut(s) 116, 153, 173, 2044
HgaI GACGC 1 cut(s) 1377
HhaI GCGC 3 cut(s) 50, 124, 648
Hin1II CATG 7 cut(s) 827, 971, 1712, 1749, 1805, 1870, 2207
Hin6I GCGC 3 cut(s) 48, 122, 646
HinP1I GCGC 3 cut(s) 48, 122, 646
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HindIII AAGCTT 1 cut(s) 543
HpaI GTTAAC 1 cut(s) 300
HphI GGTGA 9 cut(s) 338, 680, 842, 1088, 1487, 1646, 1697, 1784, 1895
Hpy166II GTNNAC 6 cut(s) 201, 300, 421, 1240, 1637, 1940
Hpy8I GTNNAC 6 cut(s) 201, 300, 421, 1240, 1637, 1940
Hpy99I CGWCG 5 cut(s) 279, 314, 317, 320, 461
HpyCH4III ACNGT 4 cut(s) 178, 409, 830, 1340
HpyCH4IV ACGT 3 cut(s) 318, 1125, 1726
HpyF10VI GCNNNNNNNGC 8 cut(s) 113, 155, 179, 387, 700, 1370, 1411, 2217
HpyF3I CTNAG 5 cut(s) 168, 290, 1092, 1613, 2039
HpySE526I ACGT 3 cut(s) 318, 1125, 1726
Hsp92II CATG 7 cut(s) 827, 971, 1712, 1749, 1805, 1870, 2207
HspAI GCGC 3 cut(s) 48, 122, 646
KroI GCCGGC 2 cut(s) 149, 381
KroNI GCCGGC 2 cut(s) 151, 383
Ksp22I TGATCA 1 cut(s) 1702
KspAI GTTAAC 1 cut(s) 300
KspI CCGCGG 1 cut(s) 120
LmnI GCTCC 4 cut(s) 96, 387, 1650, 2022
Lsp1109I GCAGC 3 cut(s) 17, 803, 1351
LweI GCATC 6 cut(s) 6, 42, 167, 261, 1903, 2065
MabI ACCWGGT 1 cut(s) 1794
MaeI CTAG 2 cut(s) 996, 2060
MaeII ACGT 3 cut(s) 318, 1125, 1726
MaeIII GTNAC 5 cut(s) 1493, 1657, 1819, 1951, 2153
MbiI CCGCTC 1 cut(s) 344
MboII GAAGA 5 cut(s) 668, 761, 1256, 1468, 1826
MfeI CAATTG 1 cut(s) 839
MflI RGATCY 8 cut(s) 75, 131, 164, 224, 336, 429, 578, 1213
MlyI GAGTC 7 cut(s) 118, 131, 195, 318, 556, 1078, 1900
MmeI TCCRAC 6 cut(s) 109, 200, 391, 480, 1526, 1585
Mph1103I ATGCAT 2 cut(s) 1722, 1872
MroNI GCCGGC 2 cut(s) 149, 381
MroXI GAANNNNTTC 1 cut(s) 1467
MseI TTAA 9 cut(s) 299, 503, 735, 776, 1194, 1245, 1878, 1901, 1967
MslI CAYNNNNRTG 3 cut(s) 828, 1725, 1924
MspA1I CMGCKG 2 cut(s) 119, 2220
MspCI CTTAAG 1 cut(s) 734
MspR9I CCNGG 9 cut(s) 88, 94, 104, 229, 358, 873, 1796, 2004, 2035
MunI CAATTG 1 cut(s) 839
Mva1269I GAATGC 1 cut(s) 1741
MvaI CCWGG 4 cut(s) 229, 1796, 2004, 2035
MvnI CGCG 2 cut(s) 50, 119
MwoI GCNNNNNNNGC 8 cut(s) 113, 155, 179, 387, 700, 1370, 1411, 2217
NaeI GCCGGC 2 cut(s) 151, 383
NciI CCSGG 5 cut(s) 88, 94, 104, 358, 873
NcoI CCATGG 2 cut(s) 1708, 1801
NdeI CATATG 1 cut(s) 1315
NgoMIV GCCGGC 2 cut(s) 149, 381
NlaIII CATG 7 cut(s) 827, 971, 1712, 1749, 1805, 1870, 2207
NlaIV GGNNCC 5 cut(s) 92, 98, 236, 664, 870
NmeAIII GCCGAG 2 cut(s) 36, 179
NmuCI GTSAC 5 cut(s) 1493, 1657, 1819, 1951, 2153
NsiI ATGCAT 2 cut(s) 1722, 1872
NspI RCATGY 1 cut(s) 1870
PaeI GCATGC 1 cut(s) 1870
PaeR7I CTCGAG 2 cut(s) 41, 1221
PagI TCATGA 2 cut(s) 823, 967
PcsI WCGNNNNNNNCGW 2 cut(s) 315, 1732
PctI GAATGC 1 cut(s) 1741
PdiI GCCGGC 2 cut(s) 151, 383
PdmI GAANNNNTTC 1 cut(s) 1467
PfeI GAWTC 5 cut(s) 271, 414, 902, 1774, 2030
PfoI TCCNGGA 2 cut(s) 86, 356
PinAI ACCGGT 1 cut(s) 665
PkrI GCNGC 7 cut(s) 32, 118, 121, 532, 793, 1366, 1404
Ple19I CGATCG 2 cut(s) 463, 526
PleI GAGTC 7 cut(s) 117, 131, 194, 318, 556, 1078, 1899
PpsI GAGTC 7 cut(s) 117, 131, 194, 318, 556, 1078, 1899
Ppu21I YACGTR 1 cut(s) 1727
PsiI TTATAA 1 cut(s) 1113
Psp6I CCWGG 4 cut(s) 227, 1794, 2002, 2033
PspGI CCWGG 4 cut(s) 227, 1794, 2002, 2033
PspN4I GGNNCC 5 cut(s) 92, 98, 236, 664, 870
PspPI GGNCC 6 cut(s) 90, 201, 869, 1306, 1798, 2047
PspXI VCTCGAGB 1 cut(s) 1221
PstI CTGCAG 1 cut(s) 2013
PsuI RGATCY 8 cut(s) 75, 131, 164, 224, 336, 429, 578, 1213
PvuI CGATCG 2 cut(s) 463, 526
PvuII CAGCTG 1 cut(s) 2220
RsaI GTAC 7 cut(s) 606, 681, 895, 1241, 1292, 1714, 2075
RsaNI GTAC 7 cut(s) 605, 680, 894, 1240, 1291, 1713, 2074
RseI CAYNNNNRTG 3 cut(s) 828, 1725, 1924
SacII CCGCGG 1 cut(s) 120
SaqAI TTAA 9 cut(s) 299, 503, 735, 776, 1194, 1245, 1878, 1901, 1967
SatI GCNGC 7 cut(s) 31, 117, 120, 531, 792, 1365, 1403
Sau96I GGNCC 6 cut(s) 90, 201, 869, 1306, 1798, 2047
ScaI AGTACT 1 cut(s) 1292
SchI GAGTC 7 cut(s) 118, 131, 195, 318, 556, 1078, 1900
ScrFI CCNGG 9 cut(s) 88, 94, 104, 229, 358, 873, 1796, 2004, 2035
SexAI ACCWGGT 1 cut(s) 1794
SfaNI GCATC 6 cut(s) 6, 42, 167, 261, 1903, 2065
SfcI CTRYAG 2 cut(s) 1395, 2009
Sfr274I CTCGAG 2 cut(s) 41, 1221
Sfr303I CCGCGG 1 cut(s) 120
SgrBI CCGCGG 1 cut(s) 120
SinI GGWCC 6 cut(s) 90, 201, 869, 1306, 1798, 2047
SlaI CTCGAG 2 cut(s) 41, 1221
SmiMI CAYNNNNRTG 3 cut(s) 828, 1725, 1924
SmlI CTYRAG 5 cut(s) 41, 425, 734, 932, 1221
SmoI CTYRAG 5 cut(s) 41, 425, 734, 932, 1221
SphI GCATGC 1 cut(s) 1870
SspMI CTAG 2 cut(s) 996, 2060
StyD4I CCNGG 9 cut(s) 86, 92, 102, 227, 356, 871, 1794, 2002, 2033
StyI CCWWGG 2 cut(s) 1708, 1801
TaaI ACNGT 4 cut(s) 178, 409, 830, 1340
TaiI ACGT 3 cut(s) 321, 1128, 1729
TaqI TCGA 7 cut(s) 42, 274, 322, 626, 786, 1222, 1772
TaqII GACCGA 2 cut(s) 1323, 1797
TatI WGTACW 4 cut(s) 679, 893, 1239, 1290
TauI GCSGC 4 cut(s) 119, 122, 533, 1405
TfiI GAWTC 5 cut(s) 271, 414, 902, 1774, 2030
Tru1I TTAA 9 cut(s) 299, 503, 735, 776, 1194, 1245, 1878, 1901, 1967
Tru9I TTAA 9 cut(s) 299, 503, 735, 776, 1194, 1245, 1878, 1901, 1967
TscAI CASTG 4 cut(s) 994, 1412, 1652, 1992
TseFI GTSAC 5 cut(s) 1493, 1657, 1819, 1951, 2153
TseI GCWGC 3 cut(s) 30, 791, 1364
Tsp45I GTSAC 5 cut(s) 1493, 1657, 1819, 1951, 2153
TspRI CASTG 4 cut(s) 994, 1412, 1652, 1992
Vha464I CTTAAG 1 cut(s) 734
VpaK11BI GGWCC 6 cut(s) 90, 201, 869, 1306, 1798, 2047
XagI CCTNNNNNAGG 1 cut(s) 1461
XapI RAATTY 5 cut(s) 467, 760, 819, 1467, 1571
XbaI TCTAGA 1 cut(s) 2059
XceI RCATGY 1 cut(s) 1870
XhoI CTCGAG 2 cut(s) 41, 1221
XmnI GAANNNNTTC 1 cut(s) 1467
XspI CTAG 2 cut(s) 996, 2060
ZrmI AGTACT 1 cut(s) 1292
Zsp2I ATGCAT 2 cut(s) 1722, 1872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.