MD06G1062300.v1.1

Oxysterol-binding protein-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001060
Physical Location & Seq
Reverse (-)
10479341 .. 10488945
9605 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1062300.v1.1.491

Sequence Viewer

Length: 2313 bp
ATGCGGGTCAAAGAGATGCACCCGCTCTGCTGCATCACGCTCGAGAGCGCGGGTCTCGGCGACCAGTCGCCGGACATGCCTCTCTCGCGGACCCGGAGCCTTCCAGCGAGTCTGGCCGCGGCGCAGGTCGGATCTGACGCAAATGCCGCCATCGGGGCATCGGGATCTGAGGCCACCGTCGCCGGAGTCCTCCACAAGTGGACGAATTACAGCAAGGGGTGGAGATCCAGGTGGTTCCTCCTCCGCAACGGCGTCCTCTCCTACGCCAAGATACGACGACCCGAAACGCTGAGCCTGTTGACCCCAAACGACGACGTTCGACTCATTGGCGAGATCTCCACTCACCGACTTTCCCAGATGGACAGCGGGACCGGAAGGCGGAGCAACAAGCCCCCGAAAAACGTTGGAATCGTCTACCTCAAGATCTCATCCTTCAGAGAAAGCAAGTCTGACGATCGGAAGTTTTACATATTTACTGCTACGAAGACCCTTCATTTAAGGACTAATTCAAAGAGCGAGCGGGCGGCTTGGCTACAAGCTTTGGTCTCAACTCGGAGTCTTTTTCCTCATAGATCCTTGAATGATAACCTCTCTCTTGTACCAACTGATTTATCTGTATCAACCGAAGGACTGAAAAAGCGCCTGCTTGAAGAAGGAACCGGTGAGGGTGTTGTACAGGATTGCGAGCGGATAATGCTCTCGGAATTCTCTCAATTACATGGACAGCTGAAGGTTCTATGTGAAGAACGATCAAATTTACTGGACACATTAAGGCAATTCGAGGCAGCCAATTATGAAGCTGAAGCCTCTGGAATTCATGATGGGGAATACCAATTGACGAAGCATGAGTTTTCTAGTTTGGGACAGGGAAAATATAGTGAGTGCAGCACAACTGAGTCATCTGACGATATTGAGAAACAAGAGCATGAGGAAGTGTCAGATGAAGACGAAATGCCATTTCATGACACACAAGAGTATTTCACTGAACCCATCATAAGTTGTGGGTCTATGAAAGGAGTGGTAACTAACAGTAATAAGCAAAGTGACACTCAAAGTCAATCTAATAAAGGTGAGGAGACACAAACTGAGAAGGAAGTTCAGGATTATAAATACCCACACGTTGAAAGAAGAAAAGAGCTTCCCACTCCAGTTGAGAAGGAGAAAGGGGTCAGCCTTTGGTCTATGATTAAAGACAATGTGGGAAAGGATCTCACTCGAGTATGCCTTCCTGTGTACTTTAATGAGCCAATATCATCTCTTCAGAAGTGTTGTGAGGATTTGGAGTACTCGTATCTTTTGGACCGTGCATATGAGTATGGGAAAATGGGAAACAGTCTCCAAAGGGTTCTAAATGTTGCTGCATTTGCGGTTTCTGGATATGCATCATCTGTAGGGCGGCACTGCAAGCCATTCAATCCCTTGTTAGGGGAAACTTATGAAGCTGACTATCCTGATAAAGGAATTCGCTTCTTCTCCGAAAAGGTTAGTCACCATCCAACACTCATTGCCTGCCATTGTGAAGGTAGAGGGTGGAAGTTCTGGGCTGACAGCAATATCCGCACTAAATTTTGGGGGCGATCAATTCAGCTTGACCCTGTTGGAGTTCTGAGCTTGCAGTTTGATGATGGTGAAATTTTCCAGTGGAGCAAGGTCACAACAAGCATATATAATCTAATTCTTGGTAAAGTGTATTGTGATCACCATGGTACAATGCATATACGTGGTAATTGTCAGCATTCATGCAAACTGAAGTTCAAAGAGCAATCGATTCTTGACCGAAATCCTCACCAGGTCCATGGATTTGTGGAAGATGTCATGGGGCAAAAGGTTGCTACGTTATTTGGGAAGTGGGATGAAAGCATGCATTATGTTAATGGTGATGGGAGTGGCAAGTTAAATCCTTCAGATGCTTCGTTGTTGTGGAAAAGTAGTAAACCTCCTGATGTCACACGATACAACTTAACGTCATTCGCAATGACACTGAATGAACTAACACCTGGACTGCAGGAGAAGCTCCCACCCACTGATTCCAGGCTCAGGCCAGACCAACGGCATCTGGAGAATGGAGAGTACGAAAAGGCAAATGCAGAGAAACAGCGGCTGGAAAAAAGGCAAAGAATGTCGAGGAAACTACAAGAAAACGGGTGGAAACCCAGGTGGTTCGAGAGAGAAGGTGAAGGTGGACCTTTCCGCTATGTTGGTGGCTATTGGGAAACACGGAAGCAGGGAAAATGGGATGAATGCCCAGACATTTTTGGTGAATTTAACGAAGGCCTTGATGATCAAACAGAAGGGAAACATAAATTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oxysterol_BP PH_11

Protein Analysis

771

Amino Acids

87.4

Weight (kDa)

6.28

Isoelectric Point (pI)

47.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 61 - 183 2e-09 PH domain
PH_11 PF15413 66 - 181 9.9e-09 Pleckstrin homology domain
Oxysterol_BP PF01237 391 - 741 9.6e-128 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1107
Acc36I ACCTGC 1 cut(s) 115
AccBSI CCGCTC 3 cut(s) 25, 520, 688
AccI GTMKAC 1 cut(s) 414
AccII CGCG 3 cut(s) 50, 88, 119
AclI AACGTT 1 cut(s) 402
AclWI GGATC 5 cut(s) 139, 172, 219, 567, 1215
AcoI YGGCCR 1 cut(s) 114
AcsI RAATTY 7 cut(s) 704, 754, 813, 1461, 1565, 1632, 2261
AcuI CTGAAG 6 cut(s) 418, 749, 822, 1244, 1769, 1887
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 6 cut(s) 600, 675, 1235, 1286, 1708, 2072
AfiI CCNNNNNNNGG 7 cut(s) 70, 153, 378, 1424, 1425, 1457, 2037
AflIII ACRYGT 1 cut(s) 1117
AgeI ACCGGT 1 cut(s) 659
AgsI TTSAA 6 cut(s) 510, 580, 650, 1124, 1414, 1756
AjnI CCWGG 5 cut(s) 227, 1788, 1996, 2030, 2153
AluBI AGCT 8 cut(s) 539, 727, 800, 1138, 1442, 1588, 1611, 2014
AluI AGCT 8 cut(s) 539, 727, 800, 1138, 1442, 1588, 1611, 2014
Alw26I GTCTC 4 cut(s) 59, 550, 1070, 1340
AlwI GGATC 5 cut(s) 139, 172, 219, 567, 1215
AlwNI CAGNNNCTG 1 cut(s) 2101
Ama87I CYCGRG 2 cut(s) 41, 1215
AoxI GGCC 4 cut(s) 114, 171, 2039, 2272
ApeKI GCWGC 4 cut(s) 30, 785, 885, 1358
ApoI RAATTY 7 cut(s) 704, 754, 813, 1461, 1565, 1632, 2261
AsiGI ACCGGT 1 cut(s) 659
AspLEI GCGC 3 cut(s) 50, 124, 642
AspS9I GGNCC 5 cut(s) 90, 369, 1300, 1792, 2183
AsuC2I CCSGG 1 cut(s) 94
AvaI CYCGRG 2 cut(s) 41, 1215
AvaII GGWCC 5 cut(s) 90, 369, 1300, 1792, 2183
BbsI GAAGAC 2 cut(s) 491, 951
BbvI GCAGC 4 cut(s) 17, 797, 897, 1345
BccI CCATC 7 cut(s) 158, 352, 815, 998, 1500, 1619, 1874
BceAI ACGGC 2 cut(s) 265, 2066
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BciT130I CCWGG 5 cut(s) 229, 1790, 1998, 2032, 2155
BclI TGATCA 2 cut(s) 1696, 2281
BcnI CCSGG 1 cut(s) 94
BcoDI GTCTC 4 cut(s) 59, 550, 1070, 1340
BfaI CTAG 1 cut(s) 855
BfmI CTRYAG 2 cut(s) 1389, 2003
BfoI RGCGCY 1 cut(s) 643
BfuAI ACCTGC 1 cut(s) 115
BglI GCCNNNNNGGC 1 cut(s) 155
BglII AGATCT 2 cut(s) 333, 423
BlpI GCTNAGC 1 cut(s) 290
BmcAI AGTACT 1 cut(s) 1286
Bme1390I CCNGG 6 cut(s) 94, 229, 1790, 1998, 2032, 2155
Bme18I GGWCC 5 cut(s) 90, 369, 1300, 1792, 2183
BmeT110I CYCGRG 2 cut(s) 41, 1215
BmgT120I GGNCC 5 cut(s) 90, 369, 1300, 1792, 2183
BmiI GGNNCC 5 cut(s) 92, 98, 236, 370, 658
BmrFI CCNGG 6 cut(s) 94, 229, 1790, 1998, 2032, 2155
BmsI GCATC 6 cut(s) 6, 42, 167, 1391, 1897, 2062
BpiI GAAGAC 2 cut(s) 491, 951
BpmI CTGGAG 2 cut(s) 1131, 2078
Bpu10I CCTNAGC 1 cut(s) 2036
Bpu1102I GCTNAGC 1 cut(s) 290
BpuEI CTTGAG 1 cut(s) 404
BpuMI CCSGG 1 cut(s) 94
Bsa29I ATCGAT 1 cut(s) 1766
BsaAI YACGTR 1 cut(s) 1721
BsaBI GATNNNNATC 1 cut(s) 1381
BsaHI GRCGYC 1 cut(s) 252
BsaI GGTCTC 2 cut(s) 59, 550
BsaJI CCNNGG 4 cut(s) 117, 1702, 1795, 2153
BsaWI WCCGGW 2 cut(s) 371, 659
BsaXI ACNNNNNCTCC 2 cut(s) 1274, 1304
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 153, 378, 1424, 1425, 1457, 2037
Bse118I RCCGGY 1 cut(s) 659
Bse1I ACTGG 4 cut(s) 64, 765, 1148, 1639
Bse3DI GCAATG 2 cut(s) 1503, 1980
Bse8I GATNNNNATC 1 cut(s) 1381
BseBI CCWGG 5 cut(s) 229, 1790, 1998, 2032, 2155
BseCI ATCGAT 1 cut(s) 1766
BseDI CCNNGG 4 cut(s) 117, 1702, 1795, 2153
BseGI GGATG 4 cut(s) 428, 1492, 1858, 2242
BseJI GATNNNNATC 1 cut(s) 1381
BseLI CCNNNNNNNGG 7 cut(s) 70, 153, 378, 1424, 1425, 1457, 2037
BseMI GCAATG 2 cut(s) 1503, 1980
BseMII CTCAG 6 cut(s) 159, 281, 885, 1077, 1598, 2050
BseNI ACTGG 4 cut(s) 64, 765, 1148, 1639
BseRI GAGGAG 2 cut(s) 230, 1088
BseXI GCAGC 4 cut(s) 17, 797, 897, 1345
BsgI GTGCAG 1 cut(s) 904
Bsh1236I CGCG 3 cut(s) 50, 88, 119
Bsh1285I CGRYCG 1 cut(s) 457
BshFI GGCC 4 cut(s) 116, 173, 2041, 2274
BshTI ACCGGT 1 cut(s) 659
BshVI ATCGAT 1 cut(s) 1766
BsiEI CGRYCG 1 cut(s) 457
BsiHKCI CYCGRG 2 cut(s) 41, 1215
BsiSI CCGG 5 cut(s) 71, 94, 183, 372, 660
BslFI GGGAC 2 cut(s) 382, 876
BslI CCNNNNNNNGG 7 cut(s) 70, 153, 378, 1424, 1425, 1457, 2037
BsmAI GTCTC 4 cut(s) 59, 550, 1070, 1340
BsmFI GGGAC 2 cut(s) 382, 876
BsmI GAATGC 2 cut(s) 1735, 2246
BsnI GGCC 4 cut(s) 116, 173, 2041, 2274
Bso31I GGTCTC 2 cut(s) 59, 550
BsoBI CYCGRG 2 cut(s) 41, 1215
Bsp1407I TGTACA 1 cut(s) 673
Bsp1720I GCTNAGC 1 cut(s) 290
Bsp19I CCATGG 2 cut(s) 1702, 1795
BspANI GGCC 4 cut(s) 116, 173, 2041, 2274
BspCNI CTCAG 6 cut(s) 160, 282, 886, 1078, 1599, 2049
BspDI ATCGAT 1 cut(s) 1766
BspFNI CGCG 3 cut(s) 50, 88, 119
BspHI TCATGA 2 cut(s) 817, 961
BspLI GGNNCC 5 cut(s) 92, 98, 236, 370, 658
BspMAI CTGCAG 1 cut(s) 2007
BspMI ACCTGC 1 cut(s) 115
BspPI GGATC 5 cut(s) 139, 172, 219, 567, 1215
BspTNI GGTCTC 2 cut(s) 59, 550
BsrBI CCGCTC 3 cut(s) 25, 520, 688
BsrDI GCAATG 2 cut(s) 1503, 1980
BsrFI RCCGGY 1 cut(s) 659
BsrGI TGTACA 1 cut(s) 673
BsrI ACTGG 4 cut(s) 64, 765, 1148, 1639
BssAI RCCGGY 1 cut(s) 659
BssECI CCNNGG 4 cut(s) 117, 1702, 1795, 2153
BssNI GRCGYC 1 cut(s) 252
BssT1I CCWWGG 2 cut(s) 1702, 1795
Bst2UI CCWGG 5 cut(s) 229, 1790, 1998, 2032, 2155
Bst4CI ACNGT 4 cut(s) 178, 1031, 1304, 1334
Bst6I CTCTTC 1 cut(s) 1263
BstACI GRCGYC 1 cut(s) 252
BstAUI TGTACA 1 cut(s) 673
BstBAI YACGTR 1 cut(s) 1721
BstC8I GCNNGC 8 cut(s) 518, 522, 644, 686, 1406, 1510, 1613, 1862
BstDEI CTNAG 6 cut(s) 168, 290, 894, 1086, 1607, 2036
BstDSI CCRYGG 3 cut(s) 117, 1702, 1795
BstENI CCTNNNNNAGG 1 cut(s) 1455
BstF5I GGATG 4 cut(s) 428, 1492, 1858, 2242
BstFNI CGCG 3 cut(s) 50, 88, 119
BstH2I RGCGCY 1 cut(s) 643
BstHHI GCGC 3 cut(s) 50, 124, 642
BstMAI GTCTC 4 cut(s) 59, 550, 1070, 1340
BstMCI CGRYCG 1 cut(s) 457
BstNI CCWGG 5 cut(s) 229, 1790, 1998, 2032, 2155
BstNSI RCATGY 2 cut(s) 79, 1864
BstSCI CCNGG 6 cut(s) 92, 227, 1788, 1996, 2030, 2153
BstSFI CTRYAG 2 cut(s) 1389, 2003
BstUI CGCG 3 cut(s) 50, 88, 119
BstV1I GCAGC 4 cut(s) 17, 797, 897, 1345
BstV2I GAAGAC 2 cut(s) 491, 951
BstX2I RGATCY 7 cut(s) 131, 164, 224, 333, 423, 572, 1207
BstXI CCANNNNNNTGG 1 cut(s) 1796
BstYI RGATCY 7 cut(s) 131, 164, 224, 333, 423, 572, 1207
Bsu15I ATCGAT 1 cut(s) 1766
BsuRI GGCC 4 cut(s) 116, 173, 2041, 2274
BsuTUI ATCGAT 1 cut(s) 1766
BtgI CCRYGG 3 cut(s) 117, 1702, 1795
BtsCI GGATG 4 cut(s) 428, 1492, 1858, 2242
BtsI GCAGTG 1 cut(s) 1399
BtsIMutI CAGTG 5 cut(s) 981, 1399, 1646, 1979, 2022
BveI ACCTGC 1 cut(s) 115
Cac8I GCNNGC 8 cut(s) 518, 522, 644, 686, 1406, 1510, 1613, 1862
CaiI CAGNNNCTG 1 cut(s) 2101
CciI TCATGA 2 cut(s) 817, 961
CfoI GCGC 3 cut(s) 50, 124, 642
Cfr10I RCCGGY 1 cut(s) 659
Cfr13I GGNCC 5 cut(s) 90, 369, 1300, 1792, 2183
Cfr42I CCGCGG 1 cut(s) 120
ClaI ATCGAT 1 cut(s) 1766
CseI GACGC 2 cut(s) 146, 241
CsiI ACCWGGT 1 cut(s) 1788
Csp6I GTAC 6 cut(s) 599, 674, 1234, 1285, 1707, 2071
CspAI ACCGGT 1 cut(s) 659
CspCI CAANNNNNGTGG 2 cut(s) 1132, 1167
CviQI GTAC 6 cut(s) 599, 674, 1234, 1285, 1707, 2071
DdeI CTNAG 6 cut(s) 168, 290, 894, 1086, 1607, 2036
EaeI YGGCCR 1 cut(s) 114
Eam1104I CTCTTC 1 cut(s) 1263
EarI CTCTTC 1 cut(s) 1263
EciI GGCGGA 1 cut(s) 394
Eco130I CCWWGG 2 cut(s) 1702, 1795
Eco147I AGGCCT 1 cut(s) 2274
Eco31I GGTCTC 2 cut(s) 59, 550
Eco47I GGWCC 5 cut(s) 90, 369, 1300, 1792, 2183
Eco57I CTGAAG 6 cut(s) 418, 749, 822, 1244, 1769, 1887
Eco88I CYCGRG 2 cut(s) 41, 1215
EcoNI CCTNNNNNAGG 1 cut(s) 1455
EcoRI GAATTC 3 cut(s) 704, 813, 1461
EcoRII CCWGG 5 cut(s) 227, 1788, 1996, 2030, 2153
EcoT14I CCWWGG 2 cut(s) 1702, 1795
EcoT22I ATGCAT 3 cut(s) 1384, 1716, 1866
ErhI CCWWGG 2 cut(s) 1702, 1795
FaqI GGGAC 2 cut(s) 382, 876
FauI CCCGC 4 cut(s) 30, 43, 359, 513
FauNDI CATATG 1 cut(s) 1309
FbaI TGATCA 2 cut(s) 1696, 2281
FblI GTMKAC 1 cut(s) 414
FokI GGATG 4 cut(s) 415, 1479, 1865, 2249
FspBI CTAG 1 cut(s) 855
GlaI GCGC 3 cut(s) 49, 123, 641
GsuI CTGGAG 2 cut(s) 1131, 2078
HaeII RGCGCY 1 cut(s) 643
HaeIII GGCC 4 cut(s) 116, 173, 2041, 2274
HapII CCGG 5 cut(s) 71, 94, 183, 372, 660
HgaI GACGC 2 cut(s) 146, 241
HhaI GCGC 3 cut(s) 50, 124, 642
Hin1I GRCGYC 1 cut(s) 252
Hin6I GCGC 3 cut(s) 48, 122, 640
HinP1I GCGC 3 cut(s) 48, 122, 640
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HindIII AAGCTT 1 cut(s) 537
HinfI GANTC 8 cut(s) 109, 186, 321, 408, 556, 896, 1768, 2027
HpaII CCGG 5 cut(s) 71, 94, 183, 372, 660
Hpy166II GTNNAC 6 cut(s) 201, 300, 415, 1234, 1934, 2183
Hpy8I GTNNAC 6 cut(s) 201, 300, 415, 1234, 1934, 2183
Hpy99I CGWCG 4 cut(s) 182, 279, 314, 317
HpyCH4III ACNGT 4 cut(s) 178, 1031, 1304, 1334
HpyCH4IV ACGT 6 cut(s) 315, 402, 1119, 1720, 1835, 1964
HpyF3I CTNAG 6 cut(s) 168, 290, 894, 1086, 1607, 2036
HpySE526I ACGT 6 cut(s) 315, 402, 1119, 1720, 1835, 1964
Hsp92I GRCGYC 1 cut(s) 252
HspAI GCGC 3 cut(s) 48, 122, 640
Ksp22I TGATCA 2 cut(s) 1696, 2281
KspI CCGCGG 1 cut(s) 120
LmnI GCTCC 4 cut(s) 96, 381, 1644, 2019
Lsp1109I GCAGC 4 cut(s) 17, 797, 897, 1345
LweI GCATC 6 cut(s) 6, 42, 167, 1391, 1897, 2062
MabI ACCWGGT 1 cut(s) 1788
MaeI CTAG 1 cut(s) 855
MaeII ACGT 6 cut(s) 315, 402, 1119, 1720, 1835, 1964
MaeIII GTNAC 5 cut(s) 1021, 1043, 1487, 1651, 1945
MbiI CCGCTC 3 cut(s) 25, 520, 688
MboII GAAGA 8 cut(s) 496, 662, 755, 956, 1140, 1250, 1462, 1820
MfeI CAATTG 1 cut(s) 833
MflI RGATCY 7 cut(s) 131, 164, 224, 333, 423, 572, 1207
MlyI GAGTC 5 cut(s) 118, 195, 315, 565, 905
MmeI TCCRAC 4 cut(s) 109, 385, 1520, 1579
Mph1103I ATGCAT 3 cut(s) 1384, 1716, 1866
MseI TTAA 8 cut(s) 497, 770, 1188, 1239, 1872, 1895, 1961, 2265
MslI CAYNNNNRTG 1 cut(s) 1719
MspA1I CMGCKG 4 cut(s) 119, 366, 727, 2098
MspI CCGG 5 cut(s) 71, 94, 183, 372, 660
MspR9I CCNGG 6 cut(s) 94, 229, 1790, 1998, 2032, 2155
MunI CAATTG 1 cut(s) 833
Mva1269I GAATGC 2 cut(s) 1735, 2246
MvaI CCWGG 5 cut(s) 229, 1790, 1998, 2032, 2155
MvnI CGCG 3 cut(s) 50, 88, 119
NciI CCSGG 1 cut(s) 94
NcoI CCATGG 2 cut(s) 1702, 1795
NdeI CATATG 1 cut(s) 1309
NlaIV GGNNCC 5 cut(s) 92, 98, 236, 370, 658
NmeAIII GCCGAG 1 cut(s) 36
NmuCI GTSAC 4 cut(s) 1043, 1487, 1651, 1945
NsiI ATGCAT 3 cut(s) 1384, 1716, 1866
NspI RCATGY 2 cut(s) 79, 1864
PaeI GCATGC 1 cut(s) 1864
PaeR7I CTCGAG 2 cut(s) 41, 1215
PagI TCATGA 2 cut(s) 817, 961
PceI AGGCCT 1 cut(s) 2274
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PctI GAATGC 2 cut(s) 1735, 2246
PfeI GAWTC 3 cut(s) 408, 1768, 2027
PinAI ACCGGT 1 cut(s) 659
Ple19I CGATCG 1 cut(s) 457
PleI GAGTC 5 cut(s) 117, 194, 315, 564, 904
PpsI GAGTC 5 cut(s) 117, 194, 315, 564, 904
Ppu21I YACGTR 1 cut(s) 1721
PsiI TTATAA 1 cut(s) 1107
Psp1406I AACGTT 1 cut(s) 402
Psp6I CCWGG 5 cut(s) 227, 1788, 1996, 2030, 2153
PspGI CCWGG 5 cut(s) 227, 1788, 1996, 2030, 2153
PspN4I GGNNCC 5 cut(s) 92, 98, 236, 370, 658
PspPI GGNCC 5 cut(s) 90, 369, 1300, 1792, 2183
PspXI VCTCGAGB 1 cut(s) 1215
PstI CTGCAG 1 cut(s) 2007
PstNI CAGNNNCTG 1 cut(s) 2101
PsuI RGATCY 7 cut(s) 131, 164, 224, 333, 423, 572, 1207
PvuI CGATCG 1 cut(s) 457
PvuII CAGCTG 1 cut(s) 727
RsaI GTAC 6 cut(s) 600, 675, 1235, 1286, 1708, 2072
RsaNI GTAC 6 cut(s) 599, 674, 1234, 1285, 1707, 2071
RseI CAYNNNNRTG 1 cut(s) 1719
SacII CCGCGG 1 cut(s) 120
SaqAI TTAA 8 cut(s) 497, 770, 1188, 1239, 1872, 1895, 1961, 2265
Sau96I GGNCC 5 cut(s) 90, 369, 1300, 1792, 2183
ScaI AGTACT 1 cut(s) 1286
SchI GAGTC 5 cut(s) 118, 195, 315, 565, 905
ScrFI CCNGG 6 cut(s) 94, 229, 1790, 1998, 2032, 2155
SexAI ACCWGGT 1 cut(s) 1788
SfaNI GCATC 6 cut(s) 6, 42, 167, 1391, 1897, 2062
SfcI CTRYAG 2 cut(s) 1389, 2003
Sfr274I CTCGAG 2 cut(s) 41, 1215
Sfr303I CCGCGG 1 cut(s) 120
SgrBI CCGCGG 1 cut(s) 120
SinI GGWCC 5 cut(s) 90, 369, 1300, 1792, 2183
SlaI CTCGAG 2 cut(s) 41, 1215
SmiMI CAYNNNNRTG 1 cut(s) 1719
SmlI CTYRAG 3 cut(s) 41, 419, 1215
SmoI CTYRAG 3 cut(s) 41, 419, 1215
SphI GCATGC 1 cut(s) 1864
SseBI AGGCCT 1 cut(s) 2274
SspMI CTAG 1 cut(s) 855
StuI AGGCCT 1 cut(s) 2274
StyD4I CCNGG 6 cut(s) 92, 227, 1788, 1996, 2030, 2153
StyI CCWWGG 2 cut(s) 1702, 1795
TaaI ACNGT 4 cut(s) 178, 1031, 1304, 1334
TaiI ACGT 6 cut(s) 318, 405, 1122, 1723, 1838, 1967
TaqI TCGA 7 cut(s) 42, 319, 780, 1216, 1766, 2123, 2163
TaqII GACCGA 1 cut(s) 1791
TatI WGTACW 3 cut(s) 673, 1233, 1284
TauI GCSGC 6 cut(s) 119, 122, 149, 527, 1399, 2101
TfiI GAWTC 3 cut(s) 408, 1768, 2027
Tru1I TTAA 8 cut(s) 497, 770, 1188, 1239, 1872, 1895, 1961, 2265
Tru9I TTAA 8 cut(s) 497, 770, 1188, 1239, 1872, 1895, 1961, 2265
TscAI CASTG 5 cut(s) 988, 1406, 1646, 1986, 2029
TseFI GTSAC 4 cut(s) 1043, 1487, 1651, 1945
TseI GCWGC 4 cut(s) 30, 785, 885, 1358
Tsp45I GTSAC 4 cut(s) 1043, 1487, 1651, 1945
TspGWI ACGGA 1 cut(s) 2233
TspRI CASTG 5 cut(s) 988, 1406, 1646, 1986, 2029
VpaK11BI GGWCC 5 cut(s) 90, 369, 1300, 1792, 2183
XagI CCTNNNNNAGG 1 cut(s) 1455
XapI RAATTY 7 cut(s) 704, 754, 813, 1461, 1565, 1632, 2261
XceI RCATGY 2 cut(s) 79, 1864
XhoI CTCGAG 2 cut(s) 41, 1215
XmiI GTMKAC 1 cut(s) 414
XspI CTAG 1 cut(s) 855
ZrmI AGTACT 1 cut(s) 1286
Zsp2I ATGCAT 3 cut(s) 1384, 1716, 1866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.