Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0011401

Physical Location & Seq

Genomic Coordinates Reverse (-)

19204873 .. 19212457

Gene Structure 7585 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1104600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4572 bp

ATGGAGAGTCGAGGTCAAAAGCGTACTGAGCTTGATGATGAATTGCCCGCAGATAAGCGGGTGTGTAATTCAATGGAGTTTAGAGCTAGTTCATCGAACTCTTCAGCTCAAACCCATATGAATTCTGTGAACTCCACACCTGAAACCAATGGCCATGATATGGATACTTCCTCTTCTGCTTCGGCTAATAGTCGCTCAGAGGGAGAACGTGAGAGGGATTCAGCATATGGGTCTTGTGATTCAGATGATGCAGACGAAAGACACAGTGAACTCAAGGACTACCAGAGACGGAGATCTTCGGGTGATCATGGAAAGTTTAAGAGGTTCATATCATCTTTAAGTGAAGAGACTGACCCTTCTGGGCAGTTAGCTGTGCTCACCGAGCTATGTGAATTCCTGTCATTTTGTACAGAGGATTCACTATCCGGGATGACATCAGACTCATTATCACCGCTTCTTGTTAGACTTGCTAGGCATGAGACTAACCCGAATATCATGCTATTGGCTATAAGGGCTATAACTTATTTATGCGATGTGTATCCGCATTCATCGGGCTTCCTTGTTAGGCATGATGCAGTTCCTGCTCTTTGCGACAGACTAATGGCTATTGAATACTTGGATGTAGCTGAACAGTGCCTACAAGCATTGGAAAAAATGTCACAGGAGCAGCCACTTGAGTGCTTACAGTCTGGGGCAATAATGGCTGTCCTGAATTATATAGATTTCTTTTCGACAAGTATACAGAGAGTTGCCCTTTCTACTGTGGTGAACATATGTAAGAAACTTCCTTCCGAATGTCCTTCACCCGTCATGGAGGCTGTTCCCATTCTTTGCAATCTTCTCCAGTGTGAGGATCCACAGCTTGTCGAAAATGTTGCTGTTTGCTTGATTAAAATTACAGAACGAGTGAGTGAGTCTACGGAGATGCTGGATGAACTTTGTAAGCATGGGCTGATTCGTCAAGTCACACATTTTATGAGCTTGAATACCCGGCCAACTGTATCCCAGCTAATTAGTAATGGTTTGATGGGGGTACTTGTCAAACTTTCTTCTGGTTCAGTTGTAGCCTTCAGGACGCTGTATGAGCTTAATATTAGCAGCATACTGAAGGATATCTTATCTACATATGACCTCTCGCATGGAATGTTCTCTAATCATGTGGTGGATGGGCATTGCAATCAGGTATATGAAGTTTTGAAATTGCTAAATGAGCTTCTTCCAACTTCGACCAGAGATCAAGAGAATCCACAGTTGTCAGATAAAGAATCTCTTTTAGTTAATCAACCTGATCTTCTGCAGAAGTTTGGGATGGATATACTTCCATTGTTGATTCAGGTTGTAAACTCCGGTGCCAATTTATATATTTGCTATGGCTGCTTATCTGTTATCGACAAGTTAATTTATTTGAGCACACCTGGCATGCTTGTAGAACTGCTTCAGAAGGCCAACATTTCAAGTTTTTTGGCTGGAGTGTTTACACGAAAGGATCCCCATGTGTTGATATTAGCTCTACAGATTGCTGAGCTAATCCTGCAGAAACTTTCTGATTATTTCTTGGACTCTTTCATAAAAGAAGGCGTCTTTTTTGCCATCGATGAACTGTTAACACCGGACAAGTGTCAATTGGTGACCCTGGAAAAATGCTCGAGGTTGTTGTTCCCTGTGTCTAGTGGCTCACAGATTTTGTTGGATCCTCGTCAGAAGTCTGCCTCGAGTGAGGTCTTGAGGTGCTTATGTTATGCTTTTGCTACTGATAAATCACCATCAGTGTCAGAAAAAGGAAGTTGCATGCTTGAAAAGGATTCTGTTTATAATCTTGCAAAGCATATAAGGACCAAGTACTTTGCACAAGAATTATGTGACCCTGAGAAAGCGTTAACCGATGTCCTTCAGAAGCTTAGAAAATGTTCTTCTGCATTAAGTGATTTGACCATGTCCATGAATGATGACGCTCTTGATCAGCATGAAGAAAGGTTCTATAGTATCATGCATCAAGTAATGGAAAAGCTTGGTGGAGGAGAACCCATTTCCACGTTTGAATTTATTGAATCTGGAATTCTAAGGTCCTTAATGACTTACCTGTCCAATGGCCAGTACTTGAAACAAAAAGGGGAGCTTAGTAGTGGTAACAGTGATATTTATTCTGTAGAAAAAAGATTTGAGGTGTTTGCAAGGCTGTTATTTTCTCCTTTAGACATGATCCCTGCTGACCTGCCCACGATAACATTAATACGCAAACTGCAAAATGCGCTATCTTCTTTGGAAAACTTCCCTGTTATTTTGAGCCATGTGCCCAAAATAAGTTCTTATGCTACAGTTCCACATGGGCGTCGCACACCATATCCATGTATTGAAGTTCGTTTTGTGAAAGACAAAGCAGAGACATGCCTTTGTGATTACTGTGAAGATGTTCTGACTGTGGACCCTTTTTCCTCATTGCATGCCATTGAAGAATTTCTGTGGCCTAAAGTCAATGCAAAAACAACCGGCCACATTAATTCACCTACACAAGTTAATGACCAATCTGAAAGTCCACTGGATCAATCACCATCAAATGCATGCTCTAGTCAAGGGCGAAGTCCACATCCTATGGAGCCTGAAAGCATGTCGACAGACTTGCCTGAGTTGCAGGAACCTGTGGAAAGAGAAGCACAATGTCCTTCAGAGGAAGACACTGAGATGGAAGAACAGTATCCTGCATCATTTAGCAATGAAGATTCTTCTTCGAAACTGTTACTTTACCTTGAAGGGCAACAGCTGGAGCCGTCATTGACATTATACCAAGCAATTCTACAACAACAAATGAAGGAACATGAAATTGTCATTGGTTCAAAACTGTGGAGTCAAGAATACACATTAACATACAGAAAAGCTGAAGGTCATCATGGTACACGTACAGAAAGTTCTGCTGAGAAAGCTGGGGTACATGAGTTGTACACTTCATTTTTCTCCAGCATGTTTGCTCATGAGCTTGCTTCTGACTTGGATAAGTCAAGTCCTATTTATGATATAATATATATCCTCAAAAGCTTGGAAGGCATGAATAAGTTTATATTTCATCTGATATCTCGAGAAAGAATATGTGCTTTTGCTAAAAGGAAAATCGATGACTTGGATAATTTTCAGACAGCAGCTATTCCAGTGCCACAGAATGAGTTTGTGAGCAAAAAATTGACAGAAAAACTGGAACAACAGATGCGGGATACTTTAGCAGTGTCCGTTGGTGGTATGCCTTTGTGGTGTAATCAGTTAATGGAATCGTGTCCCTTTTTATTTAGTTTTGAAGTTAAATGTAAGTACTTCCGCTTGGTGGCATTTAGTCCACTGCTAGTTCAGCCTCATTCACCATCTTATAGGGATTCAGGGGTGACAAGTGACAGGCGACCAAGCTCTGGAAGCATGCCTCGAAAAAAGTTTTTGGTTTTCCGCAACCAAATTTTGGATTCTGCTGCCCAAATGATGGACCTGCATGCCAGTCAGAAGGTACTCCTTGAGGTGGAATACAGTGAAGAAGTTGGTACTGGCCTTGGTCCAACCTTGGAATTCTATACCCTGGTTAGTCATGAGTTTCAGAAGTCTGGTCTAGGCATGTGGAGAGAGGATCATGGGTCTTTTGCCTCTGGTGATGGGAATACTGGAATTTTGATATGTCCTTTTGGTCTTTTCCCTCGTCCATGGTTGGCTACATCTGATGAAGTAATTAAGAAGTTTGTTCTTTTGGGGCAAATCGTAGGTAGAGCGCTCCAAGATGGGCGGGTCTTGGATGTGCATTTCTCCAAAGCCTTCTATAAGCTTATTCTTGGCAAGGAGCTTGGTGTGTATGACATCCTGTCATTTGATCCTGAGCTTGGGAAGACACTGCTAGAGTTTAAGGCTCTAGTAGACAGGAAAAGATTTTCGGAATCTGTTCCTGGAGGAAGTACAACATTGGAATTCGACTCATGCTTTCGGAAAACCCAAATTGAGGATCTTTGTCTTGATTTTACACTTCCTGGTTATCCTGATTTTGTTCTTTCCTCTCGGCCTGATCATAAAATGGTAAATATGACAAACTTGGAAGATTACGTCTCACTTGTTGCGGATACAACGGTACATGCTGGTATTTCTAGACAAGTGGAAGCTTTCAGATCTGGTTTTAATCAGGTTTTCCCTATTGAACATCTTCAGATTTTCACTGAAGAAGAATTAGAGCGTTTGCTATGTGGAGAACGTGATTCTTGGGCTTTCAATGAGCTCCTTGATCATATCAAGTTCGACCATGGTTACACAGTTAGCAGTCCTCCCATTATCAATTTGCTGGAAATCATACACAAGTTTGACCATGAACAGCGAAGAGCGTTTCTGCAATTTGTGACCGGGGCACCTCGCTTACCTCCTGGAGGTTTTGCATCTCTCAGTCCGAAGTTAACTATTGTTCGAAAGCACTCTGGCAACTGCGAAGACTTAGACCTCCCAAGCGTGATGACTTGTGCAAATTATCTTAAGCTACCACCTTACTCTTCGCAAGAGACGATGAGAGAGAAGCTCTTATATGCCATTACAGAAGGGCAGGGATCATTCCACCTTTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

HECT

Protein Analysis

1524

Amino Acids

170.95

Weight (kDa)

5.15

Isoelectric Point (pI)

53.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_TRIP12_N	PF25579	4 - 229	1.5e-114	E3 ubiquitin-protein ligase TRIP12-like, TPR repeats
HECT	PF00632	1186 - 1523	1.5e-79	HECT-domain (ubiquitin-transferase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011401)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02880 AT5G02880
fragaria_vesca	FvH4_6g15270 FvH4_6g15270 FvH4_6g15270 FvH4_6g15270
malus_domestica	MD04G1104600.v1.1 MD12G1124500.v1.1
prunus_persica	Prupe.6G241700_v2.0.a1 Prupe.6G241700_v2.0.a1
pyrus_communis	pycom12g12310
rosa_chinensis	RchiOBHm_Chr3g0468671
rosa_laevigata	RLG00000024416
rosa_multiflora	Rmu_sc0011538.1_g000007
rosa_roxburghii	Rroxscaffold_6G00412660
rosa_rugosa	Rorug03G0096200
rosa_samantha	Rh3AG145000 Rh3BG167500 Rh3DG166300
rosa_wichuraiana	Rw3G013670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1812
AasI	GACNNNNNNGTC	1 cut(s)	2080
Acc36I	ACCTGC	2 cut(s)	2220, 3474
AccB1I	GGYRCC	2 cut(s)	1349, 4360
AccB7I	CCANNNNNTGG	4 cut(s)	160, 3392, 3439, 3460
AccI	GTMKAC	4 cut(s)	739, 917, 2609, 3882
AciI	CCGC	9 cut(s)	48, 58, 452, 542, 3199, 3304, 3427, 3754, 4079
AcoI	YGGCCR	4 cut(s)	151, 992, 2089, 2488
AcsI	RAATTY	9 cut(s)	121, 392, 2039, 2055, 2453, 3435, 3542, 3639, 3932
AcuI	CTGAAG	9 cut(s)	87, 1054, 1127, 1421, 1874, 2646, 2895, 4148, 4197
AcyI	GRCGYC	2 cut(s)	1578, 2329
AfeI	AGCGCT	1 cut(s)	3741
AflII	CTTAAG	1 cut(s)	4481
AflIII	ACRYGT	1 cut(s)	2891
AhdI	GACNNNNNGTC	1 cut(s)	3829
AjnI	CCWGG	6 cut(s)	1414, 1632, 3552, 3910, 3991, 4375
AjuI	GAANNNNNNNTTGG	2 cut(s)	2808, 2840
AleI	CACNNNNGTG	1 cut(s)	676
Alw21I	GWGCWC	3 cut(s)	378, 1412, 4236
Alw26I	GTCTC	6 cut(s)	280, 341, 473, 2375, 4072, 4502
AlwNI	CAGNNNCTG	1 cut(s)	581
Ama87I	CYCGRG	3 cut(s)	1645, 1711, 3069
Aor51HI	AGCGCT	1 cut(s)	3741
AoxI	GGCC	8 cut(s)	151, 992, 1443, 2089, 2462, 2488, 3523, 4022
ApeKI	GCWGC	5 cut(s)	667, 1098, 1374, 3131, 3449
ApoI	RAATTY	9 cut(s)	121, 392, 2039, 2055, 2453, 3435, 3542, 3639, 3932
AseI	ATTAAT	2 cut(s)	2228, 2496
Asp700I	GAANNNNTTC	6 cut(s)	1434, 1906, 2409, 2454, 3906, 4161
AspLEI	GCGC	2 cut(s)	2251, 3742
AspS9I	GGNCC	5 cut(s)	1833, 2064, 2422, 3463, 3530
AsuC2I	CCSGG	3 cut(s)	427, 991, 4357
AsuII	TTCGAA	2 cut(s)	2726, 4417
AvaI	CYCGRG	3 cut(s)	1645, 1711, 3069
AvaII	GGWCC	5 cut(s)	1833, 2064, 2422, 3463, 3530
BaeGI	GKGCMC	2 cut(s)	2295, 4363
BaeI	ACNNNNGTAYC	4 cut(s)	2915, 2948, 4074, 4107
BalI	TGGCCA	2 cut(s)	153, 2091
BamHI	GGATCC	3 cut(s)	853, 1486, 1690
BanI	GGYRCC	2 cut(s)	1349, 4360
BanII	GRGCYC	1 cut(s)	4236
BarI	GAAGNNNNNNTAC	2 cut(s)	1419, 1451
BbsI	GAAGAC	3 cut(s)	2676, 3860, 4446
Bbv12I	GWGCWC	3 cut(s)	378, 1412, 4236
BbvI	GCAGC	5 cut(s)	679, 1110, 1361, 3143, 3436
BceAI	ACGGC	1 cut(s)	2749
BcgI	CGANNNNNNTGC	6 cut(s)	478, 512, 857, 891, 2599, 2633
BciT130I	CCWGG	6 cut(s)	1416, 1634, 3554, 3912, 3993, 4377
BciVI	GTATCC	6 cut(s)	157, 549, 1012, 2703, 3196, 4075
BclI	TGATCA	4 cut(s)	304, 1957, 4027, 4240
BcnI	CCSGG	3 cut(s)	427, 991, 4357
BcoDI	GTCTC	6 cut(s)	280, 341, 473, 2375, 4072, 4502
BfaI	CTAG	9 cut(s)	87, 471, 1668, 2565, 3329, 3584, 3863, 3878, 4107
BfmI	CTRYAG	6 cut(s)	1295, 1511, 1532, 1978, 2145, 2313
BfoI	RGCGCY	1 cut(s)	3743
BfrI	CTTAAG	1 cut(s)	4481
BfuAI	ACCTGC	2 cut(s)	2220, 3474
BfuI	GTATCC	6 cut(s)	157, 549, 1012, 2703, 3196, 4075
BglII	AGATCT	2 cut(s)	293, 4127
BisI	GCNGC	5 cut(s)	668, 1099, 1375, 3132, 3450
BlpI	GCTNAGC	1 cut(s)	1521
BlsI	GCNGC	5 cut(s)	669, 1100, 1376, 3133, 3451
BmcAI	AGTACT	3 cut(s)	1841, 2096, 3299
Bme1390I	CCNGG	9 cut(s)	427, 991, 1416, 1634, 3554, 3912, 3993, 4357, 4377
Bme18I	GGWCC	5 cut(s)	1833, 2064, 2422, 3463, 3530
BmeRI	GACNNNNNGTC	1 cut(s)	3829
BmeT110I	CYCGRG	3 cut(s)	1645, 1711, 3069
BmgT120I	GGNCC	5 cut(s)	1833, 2064, 2422, 3463, 3530
BmiI	GGNNCC	9 cut(s)	855, 1351, 1488, 1692, 2424, 2595, 2634, 2763, 4362
BmrFI	CCNGG	9 cut(s)	427, 991, 1416, 1634, 3554, 3912, 3993, 4357, 4377
BmsI	GCATC	7 cut(s)	238, 562, 915, 1999, 2708, 3186, 4397
BoxI	GACNNNNGTC	1 cut(s)	1617
BpiI	GAAGAC	3 cut(s)	2676, 3860, 4446
BplI	GAGNNNNNCTC	2 cut(s)	4509, 4541
BpmI	CTGGAG	6 cut(s)	827, 1488, 2780, 2935, 3933, 4398
Bpu10I	CCTNAGC	1 cut(s)	3843
Bpu1102I	GCTNAGC	1 cut(s)	1521
Bpu14I	TTCGAA	2 cut(s)	2726, 4417
BpuEI	CTTGAG	4 cut(s)	257, 695, 1744, 3512
BpuMI	CCSGG	3 cut(s)	427, 991, 4357
Bsa29I	ATCGAT	2 cut(s)	1593, 3105
BsaAI	YACGTR	1 cut(s)	2894
BsaBI	GATNNNNATC	1 cut(s)	537
BsaHI	GRCGYC	2 cut(s)	1578, 2329
BsaJI	CCNNGG	7 cut(s)	1632, 3526, 3537, 3552, 3674, 4258, 4356
BsaWI	WCCGGW	2 cut(s)	1346, 1609
Bse118I	RCCGGY	1 cut(s)	2486
Bse1I	ACTGG	8 cut(s)	844, 2092, 2541, 3140, 3189, 3474, 3526, 3640
Bse3DI	GCAATG	3 cut(s)	1171, 2435, 2716
Bse8I	GATNNNNATC	1 cut(s)	537
BseBI	CCWGG	6 cut(s)	1416, 1634, 3554, 3912, 3993, 4377
BseCI	ATCGAT	2 cut(s)	1593, 3105
BseDI	CCNNGG	7 cut(s)	1632, 3526, 3537, 3552, 3674, 4258, 4356
BseGI	GGATG	8 cut(s)	435, 625, 937, 1171, 1314, 2584, 3769, 3825
BseJI	GATNNNNATC	1 cut(s)	537
BseMI	GCAATG	3 cut(s)	1171, 2435, 2716
BseMII	CTCAG	9 cut(s)	18, 210, 1512, 1857, 2613, 2667, 2901, 3834, 4408
BseNI	ACTGG	8 cut(s)	844, 2092, 2541, 3140, 3189, 3474, 3526, 3640
BseRI	GAGGAG	1 cut(s)	2031
BseSI	GKGCMC	2 cut(s)	2295, 4363
BseXI	GCAGC	5 cut(s)	679, 1110, 1361, 3143, 3436
BseYI	CCCAGC	2 cut(s)	1005, 2918
BshFI	GGCC	8 cut(s)	153, 994, 1445, 2091, 2464, 2490, 3525, 4024
BshNI	GGYRCC	2 cut(s)	1349, 4360
BshVI	ATCGAT	2 cut(s)	1593, 3105
BsiHKAI	GWGCWC	3 cut(s)	378, 1412, 4236
BsiHKCI	CYCGRG	3 cut(s)	1645, 1711, 3069
BsiSI	CCGG	6 cut(s)	426, 991, 1347, 1610, 2487, 4356
BslFI	GGGAC	1 cut(s)	3249
BsmAI	GTCTC	6 cut(s)	280, 341, 473, 2375, 4072, 4502
BsmBI	CGTCTC	3 cut(s)	280, 4072, 4502
BsmFI	GGGAC	1 cut(s)	3249
BsmI	GAATGC	1 cut(s)	544
BsnI	GGCC	8 cut(s)	153, 994, 1445, 2091, 2464, 2490, 3525, 4024
BsoBI	CYCGRG	3 cut(s)	1645, 1711, 3069
Bsp119I	TTCGAA	2 cut(s)	2726, 4417
Bsp1286I	GDGCHC	5 cut(s)	378, 1412, 2295, 4236, 4363
Bsp1407I	TGTACA	2 cut(s)	407, 2934
Bsp1720I	GCTNAGC	1 cut(s)	1521
Bsp19I	CCATGG	2 cut(s)	3674, 4258
BspACI	CCGC	9 cut(s)	48, 58, 452, 542, 3199, 3304, 3427, 3754, 4079
BspANI	GGCC	8 cut(s)	153, 994, 1445, 2091, 2464, 2490, 3525, 4024
BspCNI	CTCAG	9 cut(s)	19, 209, 1513, 1858, 2614, 2668, 2902, 3835, 4407
BspDI	ATCGAT	2 cut(s)	1593, 3105
BspHI	TCATGA	2 cut(s)	2965, 3562
BspLI	GGNNCC	9 cut(s)	855, 1351, 1488, 1692, 2424, 2595, 2634, 2763, 4362
BspMAI	CTGCAG	2 cut(s)	1299, 1536
BspMI	ACCTGC	2 cut(s)	2220, 3474
BspQI	GCTCTTC	1 cut(s)	4327
BspT104I	TTCGAA	2 cut(s)	2726, 4417
BspT107I	GGYRCC	2 cut(s)	1349, 4360
BspTI	CTTAAG	1 cut(s)	4481
BsrDI	GCAATG	3 cut(s)	1171, 2435, 2716
BsrFI	RCCGGY	1 cut(s)	2486
BsrGI	TGTACA	2 cut(s)	407, 2934
BsrI	ACTGG	8 cut(s)	844, 2092, 2541, 3140, 3189, 3474, 3526, 3640
BssAI	RCCGGY	1 cut(s)	2486
BssECI	CCNNGG	7 cut(s)	1632, 3526, 3537, 3552, 3674, 4258, 4356
BssNAI	GTATAC	1 cut(s)	740
BssNI	GRCGYC	2 cut(s)	1578, 2329
BssT1I	CCWWGG	4 cut(s)	3526, 3537, 3674, 4258
Bst1107I	GTATAC	1 cut(s)	740
Bst2UI	CCWGG	6 cut(s)	1416, 1634, 3554, 3912, 3993, 4377
Bst6I	CTCTTC	5 cut(s)	106, 178, 339, 4327, 4504
BstACI	GRCGYC	2 cut(s)	1578, 2329
BstAFI	CTTAAG	1 cut(s)	4481
BstAPI	GCANNNNNTGC	1 cut(s)	581
BstAUI	TGTACA	2 cut(s)	407, 2934
BstBAI	YACGTR	1 cut(s)	2894
BstBI	TTCGAA	2 cut(s)	2726, 4417
BstC8I	GCNNGC	8 cut(s)	48, 1421, 1790, 2442, 2560, 2973, 3401, 3471
BstDSI	CCRYGG	2 cut(s)	3674, 4258
BstEII	GGTNACC	1 cut(s)	1627
BstENI	CCTNNNNNAGG	1 cut(s)	4377
BstF5I	GGATG	8 cut(s)	435, 625, 937, 1171, 1314, 2584, 3769, 3825
BstH2I	RGCGCY	1 cut(s)	3743
BstHHI	GCGC	2 cut(s)	2251, 3742
BstMAI	GTCTC	6 cut(s)	280, 341, 473, 2375, 4072, 4502
BstNI	CCWGG	6 cut(s)	1416, 1634, 3554, 3912, 3993, 4377
BstPAI	GACNNNNGTC	1 cut(s)	1617
BstPI	GGTNACC	1 cut(s)	1627
BstSCI	CCNGG	9 cut(s)	425, 989, 1414, 1632, 3552, 3910, 3991, 4355, 4375
BstSFI	CTRYAG	6 cut(s)	1295, 1511, 1532, 1978, 2145, 2313
BstSLI	GKGCMC	2 cut(s)	2295, 4363
BstV1I	GCAGC	5 cut(s)	679, 1110, 1361, 3143, 3436
BstV2I	GAAGAC	3 cut(s)	2676, 3860, 4446
BstX2I	RGATCY	6 cut(s)	293, 853, 1486, 1690, 3967, 4127
BstYI	RGATCY	6 cut(s)	293, 853, 1486, 1690, 3967, 4127
BstZ17I	GTATAC	1 cut(s)	740
Bsu15I	ATCGAT	2 cut(s)	1593, 3105
BsuI	GTATCC	6 cut(s)	157, 549, 1012, 2703, 3196, 4075
BsuRI	GGCC	8 cut(s)	153, 994, 1445, 2091, 2464, 2490, 3525, 4024
BsuTUI	ATCGAT	2 cut(s)	1593, 3105
BtgI	CCRYGG	2 cut(s)	3674, 4258
BtgZI	GCGATG	1 cut(s)	546
BtsCI	GGATG	8 cut(s)	435, 625, 937, 1171, 1314, 2584, 3769, 3825
BtsI	GCAGTG	3 cut(s)	3219, 3323, 3857
BveI	ACCTGC	2 cut(s)	2220, 3474
Cac8I	GCNNGC	8 cut(s)	48, 1421, 1790, 2442, 2560, 2973, 3401, 3471
CaiI	CAGNNNCTG	1 cut(s)	581
CciI	TCATGA	2 cut(s)	2965, 3562
CfoI	GCGC	2 cut(s)	2251, 3742
Cfr10I	RCCGGY	1 cut(s)	2486
Cfr13I	GGNCC	5 cut(s)	1833, 2064, 2422, 3463, 3530
ClaI	ATCGAT	2 cut(s)	1593, 3105
CseI	GACGC	4 cut(s)	1084, 1567, 1958, 2318
DrdI	GACNNNNNNGTC	1 cut(s)	2080
DriI	GACNNNNNGTC	1 cut(s)	3829
DseDI	GACNNNNNNGTC	1 cut(s)	2080
EaeI	YGGCCR	4 cut(s)	151, 992, 2089, 2488
Eam1104I	CTCTTC	5 cut(s)	106, 178, 339, 4327, 4504
Eam1105I	GACNNNNNGTC	1 cut(s)	3829
EarI	CTCTTC	5 cut(s)	106, 178, 339, 4327, 4504
Ecl136II	GAGCTC	1 cut(s)	4234
Eco130I	CCWWGG	4 cut(s)	3526, 3537, 3674, 4258
Eco24I	GRGCYC	1 cut(s)	4236
Eco32I	GATATC	2 cut(s)	1114, 3066
Eco47I	GGWCC	5 cut(s)	1833, 2064, 2422, 3463, 3530
Eco47III	AGCGCT	1 cut(s)	3741
Eco53kI	GAGCTC	1 cut(s)	4234
Eco57I	CTGAAG	9 cut(s)	87, 1054, 1127, 1421, 1874, 2646, 2895, 4148, 4197
Eco88I	CYCGRG	3 cut(s)	1645, 1711, 3069
Eco91I	GGTNACC	1 cut(s)	1627
EcoICRI	GAGCTC	1 cut(s)	4234
EcoNI	CCTNNNNNAGG	1 cut(s)	4377
EcoO109I	RGGNCCY	1 cut(s)	2064
EcoO65I	GGTNACC	1 cut(s)	1627
EcoRI	GAATTC	5 cut(s)	121, 392, 2055, 3542, 3932
EcoRII	CCWGG	6 cut(s)	1414, 1632, 3552, 3910, 3991, 4375
EcoRV	GATATC	2 cut(s)	1114, 3066
EcoT14I	CCWWGG	4 cut(s)	3526, 3537, 3674, 4258
EcoT22I	ATGCAT	2 cut(s)	1992, 2560
EcoT38I	GRGCYC	1 cut(s)	4236
ErhI	CCWWGG	4 cut(s)	3526, 3537, 3674, 4258
Esp3I	CGTCTC	3 cut(s)	280, 4072, 4502
FalI	AAGNNNNNCTT	6 cut(s)	1100, 1132, 1254, 1286, 2100, 2132
FaqI	GGGAC	1 cut(s)	3249
FauI	CCCGC	4 cut(s)	51, 55, 3192, 3747
FauNDI	CATATG	4 cut(s)	117, 226, 773, 1126
FbaI	TGATCA	4 cut(s)	304, 1957, 4027, 4240
FblI	GTMKAC	4 cut(s)	739, 917, 2609, 3882
Fnu4HI	GCNGC	5 cut(s)	668, 1099, 1375, 3132, 3450
FokI	GGATG	8 cut(s)	442, 632, 944, 1178, 1321, 2571, 3776, 3812
FriOI	GRGCYC	1 cut(s)	4236
Fsp4HI	GCNGC	5 cut(s)	668, 1099, 1375, 3132, 3450
FspBI	CTAG	9 cut(s)	87, 471, 1668, 2565, 3329, 3584, 3863, 3878, 4107
GlaI	GCGC	2 cut(s)	2250, 3741
GluI	GCNGC	5 cut(s)	668, 1099, 1375, 3132, 3450
GsaI	CCCAGC	2 cut(s)	1009, 2922
GsuI	CTGGAG	6 cut(s)	827, 1488, 2780, 2935, 3933, 4398
HaeII	RGCGCY	1 cut(s)	3743
HaeIII	GGCC	8 cut(s)	153, 994, 1445, 2091, 2464, 2490, 3525, 4024
HapII	CCGG	6 cut(s)	426, 991, 1347, 1610, 2487, 4356
HgaI	GACGC	4 cut(s)	1084, 1567, 1958, 2318
HhaI	GCGC	2 cut(s)	2251, 3742
Hin1I	GRCGYC	2 cut(s)	1578, 2329
Hin6I	GCGC	2 cut(s)	2249, 3740
HinP1I	GCGC	2 cut(s)	2249, 3740
HincII	GTYRAC	4 cut(s)	1605, 1878, 2610, 4407
HindII	GTYRAC	4 cut(s)	1605, 1878, 2610, 4407
HindIII	AAGCTT	5 cut(s)	1895, 2006, 3028, 3791, 4119
HpaI	GTTAAC	3 cut(s)	1605, 1878, 4407
HpaII	CCGG	6 cut(s)	426, 991, 1347, 1610, 2487, 4356
Hpy99I	CGWCG	1 cut(s)	2334
HpyCH4IV	ACGT	5 cut(s)	208, 2033, 2893, 4065, 4210
HpySE526I	ACGT	5 cut(s)	208, 2033, 2893, 4065, 4210
Hsp92I	GRCGYC	2 cut(s)	1578, 2329
HspAI	GCGC	2 cut(s)	2249, 3740
Ksp22I	TGATCA	4 cut(s)	304, 1957, 4027, 4240
KspAI	GTTAAC	3 cut(s)	1605, 1878, 4407
LguI	GCTCTTC	1 cut(s)	4327
LmnI	GCTCC	7 cut(s)	664, 2113, 2593, 2761, 3747, 3808, 4239
Lsp1109I	GCAGC	5 cut(s)	679, 1110, 1361, 3143, 3436
LweI	GCATC	7 cut(s)	238, 562, 915, 1999, 2708, 3186, 4397
MaeI	CTAG	9 cut(s)	87, 471, 1668, 2565, 3329, 3584, 3863, 3878, 4107
MaeII	ACGT	5 cut(s)	208, 2033, 2893, 4065, 4210
MfeI	CAATTG	1 cut(s)	1622
MflI	RGATCY	6 cut(s)	293, 853, 1486, 1690, 3967, 4127
MhlI	GDGCHC	5 cut(s)	378, 1412, 2295, 4236, 4363
MlsI	TGGCCA	2 cut(s)	153, 2091
MluNI	TGGCCA	2 cut(s)	153, 2091
MlyI	GAGTC	6 cut(s)	16, 434, 923, 1553, 2851, 3932
MmeI	TCCRAC	3 cut(s)	1244, 1668, 3557
Mox20I	TGGCCA	2 cut(s)	153, 2091
Mph1103I	ATGCAT	2 cut(s)	1992, 2560
MroXI	GAANNNNTTC	6 cut(s)	1434, 1906, 2409, 2454, 3906, 4161
MscI	TGGCCA	2 cut(s)	153, 2091
MslI	CAYNNNNRTG	4 cut(s)	676, 1937, 2344, 2678
Msp20I	TGGCCA	2 cut(s)	153, 2091
MspA1I	CMGCKG	1 cut(s)	2758
MspCI	CTTAAG	1 cut(s)	4481
MspI	CCGG	6 cut(s)	426, 991, 1347, 1610, 2487, 4356
MspR9I	CCNGG	9 cut(s)	427, 991, 1416, 1634, 3554, 3912, 3993, 4357, 4377
MunI	CAATTG	1 cut(s)	1622
Mva1269I	GAATGC	1 cut(s)	544
MvaI	CCWGG	6 cut(s)	1416, 1634, 3554, 3912, 3993, 4377
NciI	CCSGG	3 cut(s)	427, 991, 4357
NcoI	CCATGG	2 cut(s)	3674, 4258
NdeI	CATATG	4 cut(s)	117, 226, 773, 1126
NlaIV	GGNNCC	9 cut(s)	855, 1351, 1488, 1692, 2424, 2595, 2634, 2763, 4362
NmeAIII	GCCGAG	1 cut(s)	4000
NmuCI	GTSAC	7 cut(s)	657, 964, 1627, 1859, 3367, 3374, 4351
NsiI	ATGCAT	2 cut(s)	1992, 2560
NspV	TTCGAA	2 cut(s)	2726, 4417
OliI	CACNNNNGTG	1 cut(s)	676
PaeI	GCATGC	6 cut(s)	1423, 1792, 2444, 2562, 3403, 3473
PaeR7I	CTCGAG	3 cut(s)	1645, 1711, 3069
PagI	TCATGA	2 cut(s)	2965, 3562
PciSI	GCTCTTC	1 cut(s)	4327
PcsI	WCGNNNNNNNCGW	1 cut(s)	4508
PctI	GAATGC	1 cut(s)	544
PdmI	GAANNNNTTC	6 cut(s)	1434, 1906, 2409, 2454, 3906, 4161
PflFI	GACNNNGTC	1 cut(s)	1933
PflMI	CCANNNNNTGG	4 cut(s)	160, 3392, 3439, 3460
PfoI	TCCNGGA	3 cut(s)	425, 3910, 4375
PkrI	GCNGC	5 cut(s)	669, 1100, 1376, 3133, 3451
PleI	GAGTC	6 cut(s)	15, 434, 922, 1553, 2850, 3932
PpsI	GAGTC	6 cut(s)	15, 434, 922, 1553, 2850, 3932
Ppu21I	YACGTR	1 cut(s)	2894
PpuMI	RGGWCCY	1 cut(s)	2064
PshAI	GACNNNNGTC	1 cut(s)	1617
PshBI	ATTAAT	2 cut(s)	2228, 2496
PsiI	TTATAA	1 cut(s)	1812
Psp124BI	GAGCTC	1 cut(s)	4236
Psp5II	RGGWCCY	1 cut(s)	2064
Psp6I	CCWGG	6 cut(s)	1414, 1632, 3552, 3910, 3991, 4375
PspEI	GGTNACC	1 cut(s)	1627
PspFI	CCCAGC	2 cut(s)	1005, 2918
PspGI	CCWGG	6 cut(s)	1414, 1632, 3552, 3910, 3991, 4375
PspN4I	GGNNCC	9 cut(s)	855, 1351, 1488, 1692, 2424, 2595, 2634, 2763, 4362
PspPI	GGNCC	5 cut(s)	1833, 2064, 2422, 3463, 3530
PspPPI	RGGWCCY	1 cut(s)	2064
PspXI	VCTCGAGB	2 cut(s)	1645, 1711
PsrI	GAACNNNNNNTAC	4 cut(s)	621, 653, 2887, 2919
PstI	CTGCAG	2 cut(s)	1299, 1536
PstNI	CAGNNNCTG	1 cut(s)	581
PsuI	RGATCY	6 cut(s)	293, 853, 1486, 1690, 3967, 4127
PsyI	GACNNNGTC	1 cut(s)	1933
PvuII	CAGCTG	1 cut(s)	2758
RseI	CAYNNNNRTG	4 cut(s)	676, 1937, 2344, 2678
SacI	GAGCTC	1 cut(s)	4236
SalI	GTCGAC	1 cut(s)	2608
SapI	GCTCTTC	1 cut(s)	4327
SatI	GCNGC	5 cut(s)	668, 1099, 1375, 3132, 3450
Sau96I	GGNCC	5 cut(s)	1833, 2064, 2422, 3463, 3530
ScaI	AGTACT	3 cut(s)	1841, 2096, 3299
SchI	GAGTC	6 cut(s)	16, 434, 923, 1553, 2851, 3932
ScrFI	CCNGG	9 cut(s)	427, 991, 1416, 1634, 3554, 3912, 3993, 4357, 4377
SduI	GDGCHC	5 cut(s)	378, 1412, 2295, 4236, 4363
SfaNI	GCATC	7 cut(s)	238, 562, 915, 1999, 2708, 3186, 4397
SfcI	CTRYAG	6 cut(s)	1295, 1511, 1532, 1978, 2145, 2313
Sfr274I	CTCGAG	3 cut(s)	1645, 1711, 3069
SfuI	TTCGAA	2 cut(s)	2726, 4417
SinI	GGWCC	5 cut(s)	1833, 2064, 2422, 3463, 3530
SlaI	CTCGAG	3 cut(s)	1645, 1711, 3069
SmiMI	CAYNNNNRTG	4 cut(s)	676, 1937, 2344, 2678
SmlI	CTYRAG	8 cut(s)	272, 674, 1645, 1711, 1723, 3069, 3491, 4481
SmoI	CTYRAG	8 cut(s)	272, 674, 1645, 1711, 1723, 3069, 3491, 4481
SphI	GCATGC	6 cut(s)	1423, 1792, 2444, 2562, 3403, 3473
SsiI	CCGC	9 cut(s)	48, 58, 452, 542, 3199, 3304, 3427, 3754, 4079
SspI	AATATT	1 cut(s)	1093
SspMI	CTAG	9 cut(s)	87, 471, 1668, 2565, 3329, 3584, 3863, 3878, 4107
SstI	GAGCTC	1 cut(s)	4236
StyD4I	CCNGG	9 cut(s)	425, 989, 1414, 1632, 3552, 3910, 3991, 4355, 4375
StyI	CCWWGG	4 cut(s)	3526, 3537, 3674, 4258
TaiI	ACGT	5 cut(s)	211, 2036, 2896, 4068, 4213
TatI	WGTACW	6 cut(s)	407, 1839, 2094, 2934, 3297, 3920
TseFI	GTSAC	7 cut(s)	657, 964, 1627, 1859, 3367, 3374, 4351
TseI	GCWGC	5 cut(s)	667, 1098, 1374, 3131, 3449
Tsp45I	GTSAC	7 cut(s)	657, 964, 1627, 1859, 3367, 3374, 4351
TspGWI	ACGGA	3 cut(s)	304, 935, 3208
Tth111I	GACNNNGTC	1 cut(s)	1933
Van91I	CCANNNNNTGG	4 cut(s)	160, 3392, 3439, 3460
Vha464I	CTTAAG	1 cut(s)	4481
VpaK11BI	GGWCC	5 cut(s)	1833, 2064, 2422, 3463, 3530
VspI	ATTAAT	2 cut(s)	2228, 2496
XagI	CCTNNNNNAGG	1 cut(s)	4377
XapI	RAATTY	9 cut(s)	121, 392, 2039, 2055, 2453, 3435, 3542, 3639, 3932
XbaI	TCTAGA	1 cut(s)	4106
XhoI	CTCGAG	3 cut(s)	1645, 1711, 3069
XmiI	GTMKAC	4 cut(s)	739, 917, 2609, 3882
XmnI	GAANNNNTTC	6 cut(s)	1434, 1906, 2409, 2454, 3906, 4161
XspI	CTAG	9 cut(s)	87, 471, 1668, 2565, 3329, 3584, 3863, 3878, 4107
ZrmI	AGTACT	3 cut(s)	1841, 2096, 3299
Zsp2I	ATGCAT	2 cut(s)	1992, 2560

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.