Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0011401

Physical Location & Seq

Genomic Coordinates Reverse (-)

35609472 .. 35615969

Gene Structure 6498 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000024416

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4719 bp

ATGGAAAGTCGTGGCCAAAAGCGGTCTGAGATTGATGATGAATTGCCGGCCGATAAGAGGGCCTGTAGTTCTTTGGAGTTTAGACCGAGTACATCCAGCTCGTCTCAAACCCCTATCAATTCTGTGAGCTCCACGCCCGAACGCAATGATCAAAACAATGATCAGAACAATAATTATCATGCTGATCATATGGATACTGAATCCGAAGGAGGAGAGCCTGAGAAGGATTCGGCGTATGATTCTTGTGATGAGGATGAGGAAGATCAAAGACACAGTGATCTTAGGGACTATCAGAGGCGGAGAGCATCCGGTGATCATGGAAGGTTTCAGACCATAATATCATCTCTGAGTGAAGAAGTCGATCTTTCTCAGCACCTGGTTATGCTCACCGAGTTATGCGAAGTGTTATCTTTTTGTACAGAGGATTCACTGTCAGGTGATACATCGAACTCGTTGTCACCGATTCTTGTTAAGCTGGCAAGGGATGAGACTAGCGCGGATATCATGCTATTGGCTATAAGGGCAATGACTTATTTATGTGATGTATATCCAAAATCATCGGTGTACTTGGTCCGACATGATGCAGTTCCTGCTCTTTGCCAAAGACTTTTGGCTATTGAGTATTTGGATGTAGCTGAGCAATGTCTACAAGCATTGGAGAAGTTGTCACGGGAGCAACCACTTGCATGCTTACAGGCTGGAGCAATAATGGCTGTTCTGAATTATATAGACTTCTTCTCTACAAGCATACAGAGAGTTGCCCTTTCTACTGTGGTGAACATTTGTAAGAAACTTCCTTCTGAAGGTCCTTCACCTTTCATGGATGCTGTTCCTATATTGTGCAACCTTCTTCAATATGAGGATCCACAGCTTGTCGAGAATGTTGCTATTTGCTTGATTAGAATTACAGAGCGAGTGAGTCAGTCTTCTGAGATGCTGGATGAACTTTGTAAGCATGGGCTGATTCGTCAAGCCACACATTTTATGAGCTTAAATAGCCGAACTACTTTGTCCCAACCAATTCATAATGGTTTGATAGGGCTACTTGTCAAACTTTCTTCCGGTTCAGTTTTAGCCTTCAGAACTCTATATGAGCTTAATATAAGCAGCATATTAAAGGATATATTATCCACTTATGACCTCTCACATGGAATGTCCTCTACTCATGTTGTGGATGGGCATTGCTATCAGGTATATGAAGTTCTGAAGTTACTAAATGAGCTTCTCCCAACTTCAACCAGAGATCAAGATGCTCCACAGTTGTCAGAGAAGGAATCTTTTTTAATAAATCAGCCTGAACTTCTGGAGAACTTTGGGAGGGATATACTTCCGTTGCTGATACAGGTTGTAAATTCTGGTGCCAATTTATATATTTGCCATGGATGCTTGTCAGTCATCAACAAGTTAATTTATTTGAGCACATCTGACATGCTCGTTGAACTGCTTAAGAATGCCAACATCTCAAGTTTTCTGGCTGGAGTCTTTACTCGAAAGGATCCTCATGTGTTGGTATCAACCCTACGGATTGCTGAGTTAATCCTGCAAAAGTTCTCTGATAATTTCTTGGACTCATTTATTAAAGAAGGTGTATTTTTTGCCATTGATGCTTTGCTAACCCCAGAAAAATGCTCACTAGTAACACTGAACAAATGTTCGAAGCTGGTGTTTCCAGTGTCTAGTGGAGCAAGGCTGCTGTCTGACTCCAGTCAGAAGTCTGCTTCAAAAGAGGTCCTGAGATGCTTATGTTATGCTTTTCCTAGCAGTTCCCCATCAGGGTCAGAAAAAGGAAGTTGCATGCTTGAAAAGGATTCTGTTTATAGTCTTGCAAAGCATGTTAGAACCAAGTACTTTGCACCAGAATTATGTGACCCTGATAAATCATTAACTGATGTTCTTCAGAAGCTTAGAACTTTTTCTGCTTCATTAAGTGATTTGATGAACATGTCCTTGGATACTTGTGCTCCTAATCAGCATGAAGACAGCTTCTATGGTATAATGCATCAAGTAATGGAAAAGCTTAGTGGAAGAGAACCCATTTCCACTTTTGAATTGATTGAAAGTGGAATTCTGAAGTCCTTGATGACTTACCTGTCCAATGACCGGTATTTGAGACAGAAGGGTGATCTTGTAGCTGCTAAAGGTGATATCTATGCTGTAGAAAAAAGATTTGAGGTGTTCGCAAGGCTATTATTTTCTTCGCCAGATCCGTTCTCTGGTGACCTGCCCATTGTAACATTGATACGGAGATTACAAAGCTCACTATCTACTTTGGAAAATTTCCCCGTTATCTTGAGCCATATACCCAAGCAGAGGAACTCGTATGCTACAGTTCCGTATGATCGATACACTGCATATCCATGTATGAGAGCTCGTTTTGTTAGAGACAAGGAAGAGACTACCCTTGGTGATTGCTGTGAAGATGTTTTGACAGTTGACCCTTTTTCTTCATTGGATGCCATTGAACGATATGTGTGGCCTAAAGTCAATGCAAAAGGAACCAGCCGCATCAAATCAGCTACAGTTGAGGGTCAATCAGAATGCACACCATCAAATGCCAGCTCTAGTCAAGGAGGGTGTCCAAATACTGGTGAGCCTGAGAGCATGTCCGCAGACTTGCCTGAGTTGAAGGCAGATGAGGTCAATTTAACACAGCCGGAACCTGAACAAGAACCAAGTTTTGGACAGACAAATCCTGGAACATCTTTAGATGAAACTTATACTGATACTGTGGAGAAGGAAGTAGAAGCTCAATCTGAGGAAGACACACAGATGGAAGAACAGTGTCCTTTATCTTGTAGCAATGAAGATGCTTCACCGAAATTGTTGTTTTACCTTGAAGGGAAACAGCTGGAACGGTCATTGACGTTGTATCAGGCAATTCTTCAACAGCAAATGAAAGAACATGAAATTGTCATTGGTTCAAAACTGTGGAGTAAAGTATACACATTGACATATAGAAAAGCTGTAGGTCAAGAAGGTGCTCGTAATGAAGGTGTTGACTCGGCAGAGAGTTCCACTGTATCAGATAAAGTTGGGGTGTATACGTTGTATACTTCACTTTTCTCCAACATGTTTCCTTGTGAGCTTGCTTCTGACTTGGAGAAGTTGAATCCTACCTATGACATTGTATATCTGCTCAAAAGCTTGGAAAGTATGAACAAGTTTATTTTTCATCTGATGTCTCGTCAAAGAATATGTGCTTTTGTTGAAGGGAAAATCAATGACTTGGATGCATTTCAGATAGCAGTTACACCAGTGCCACAGAATGAGTTTTTGAGCAGTAAACTGACCGAAAAACTAGAACAGCAGATGCGGGATGGTTTGGCTGTGTCCGTTGGTGGTATGCCTCTGTGGTGTAATCAATTAATGGCATCGTGTCCTTTTTTGTTTAGTTTTGAGGTCAAATGCAAGTATTTCCGCTTGGCAGCCTTTGTTCCACTACTAGGTCAGCCTCCTTCACCGTCTCACGGTGATTCAGGTATGACAAGTGACAGGCGACAAAGCTCTGGTGGCTTACCTCGACAAAAGTTTTTAGTTTTCCGCAACCGCATTCTGGATTCTGCTGCCCAAATGATGGAGCTGCATGCCTGTCAGAAGGTACTTCTTGAGGTGGAATATGATGAAGAAGTTGGTACTGGTCTTGGTCCAACATTGGAATTTTATACCCTGGTTAGTCATGAGTTTCAGAAATCTGGCCTGGGCATGTGGAGAGAGGATGGTGGATCTTTTACCTCTGGTATAAGCCATGCTGAGGATACTGGAATTTTGATGAGTCCTTGTGGTCTCTTTCCTCGTCCGTGGTCATCTACTATGGATGCATCTGATGGGACACAGTTCAGTGAAGTAATCAAGAAGTTTGCGCTTTTGGGAAAAATTGTAGGGAAAGCGCTTCAGGATGGGAGGGTCTTGGATCTGCATTTCTCCAAAGCCTTTTATAAACTAATTCTTGGACAGGACCTTGGTTTGTATGACATCCAGTCATTTGATCCTGTGCTTGGAAGGACACTACTAGAGTTCAAGGCTCTTGTGGAAAGGAAAAGGTTTTTGGAATCTGTACATGGAGAAAATACAACATTTGAGTCTGACTTGTGCTTCAGGAAAACTAGAATTGAGGATCTTTGCCTTGATTTTACACTTCCTGGGTATCCTGATTTTGTCCTTGCATCCGGGTTTGATCATAAAATGGTAAATTCCACGAACTTGGAAGATTATGTTTCACTTATGGCAGATGCAACTATAAATTCTGGAATTTCCAGACAAGTGGAAGCTTTCAAGTCTGGTTTTAACCAGGTGTTCCCTATTGAGCATCTTCAAATTTTCACTGAAGAAGAACTAGAGCGTCTACTATGTGGAGAACGTGATTCTTGGGCTTTCAATGAGCTTCTTGATCATATCAAATTTGACCATGGTTACACTGCTAGCAGTCCTCCCATTGTTAATTTGCTGGAAATCATACACGAGTTTGACCAAGAACAGCGAAGAGCGTTTCTGCAATTTGTGACTGGGGCACCTCGCTTACCTCCAGGAGGTTTTGCATCCCTCAATCCGAAGTTAACTATTGTTCGAAAGCACTCTAGCAACTCGGCGGACTTGGACCTACCAAGTGTGATGACTTGTGCAAACTATCTTAAGCTGCCACCGTACTCTTCAAAAGAGATGATGAAGGAGAAGCTCGTGTATGCCATTAAAGAAGGGCAGGGATCATTCCACCTTTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

HECT

Protein Analysis

1573

Amino Acids

175.64

Weight (kDa)

5.0

Isoelectric Point (pI)

50.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_TRIP12_N	PF25579	4 - 232	1.5e-102	E3 ubiquitin-protein ligase TRIP12-like, TPR repeats
HECT	PF00632	1259 - 1572	3.9e-82	HECT-domain (ubiquitin-transferase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011401)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02880 AT5G02880
fragaria_vesca	FvH4_6g15270 FvH4_6g15270 FvH4_6g15270 FvH4_6g15270
malus_domestica	MD04G1104600.v1.1 MD12G1124500.v1.1
prunus_persica	Prupe.6G241700_v2.0.a1 Prupe.6G241700_v2.0.a1
pyrus_communis	pycom12g12310
rosa_chinensis	RchiOBHm_Chr3g0468671
rosa_laevigata	RLG00000024416
rosa_multiflora	Rmu_sc0011538.1_g000007
rosa_roxburghii	Rroxscaffold_6G00412660
rosa_rugosa	Rorug03G0096200
rosa_samantha	Rh3AG145000 Rh3BG167500 Rh3DG166300
rosa_wichuraiana	Rw3G013670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3936
AasI	GACNNNNNNGTC	1 cut(s)	2089
Acc36I	ACCTGC	1 cut(s)	2229
AccB1I	GGYRCC	2 cut(s)	1358, 4507
AccB7I	CCANNNNNTGG	3 cut(s)	2585, 2678, 3574
AccI	GTMKAC	5 cut(s)	646, 2940, 3041, 3050, 4342
AccII	CGCG	1 cut(s)	497
AcoI	YGGCCR	2 cut(s)	13, 48
AcuI	CTGAAG	8 cut(s)	822, 1063, 1226, 1880, 2090, 3875, 4078, 4344
AfaI	GTAC	8 cut(s)	91, 418, 566, 1847, 3600, 3634, 4056, 4643
AfeI	AGCGCT	1 cut(s)	3888
AflII	CTTAAG	2 cut(s)	1445, 4628
AflIII	ACRYGT	2 cut(s)	1941, 3069
AgeI	ACCGGT	1 cut(s)	2100
AhdI	GACNNNNNGTC	1 cut(s)	3976
AhlI	ACTAGT	1 cut(s)	1633
AjnI	CCWGG	7 cut(s)	375, 2692, 3666, 3696, 4138, 4287, 4522
AjuI	GAANNNNNNNTTGG	8 cut(s)	1931, 1963, 2661, 2693, 2898, 2930, 4028, 4060
Alw21I	GWGCWC	5 cut(s)	131, 1421, 1963, 2371, 2983
Alw26I	GTCTC	8 cut(s)	108, 482, 2104, 2376, 2387, 3186, 3468, 3788
AlwNI	CAGNNNCTG	2 cut(s)	376, 590
Aor51HI	AGCGCT	1 cut(s)	3888
AoxI	GGCC	5 cut(s)	13, 48, 60, 2474, 3694
ApeKI	GCWGC	7 cut(s)	1107, 1690, 2132, 3425, 3563, 3580, 4633
ArsI	GACNNNNNNTTYG	2 cut(s)	440, 472
AseI	ATTAAT	1 cut(s)	3365
AsiGI	ACCGGT	1 cut(s)	2100
Asp700I	GAANNNNTTC	1 cut(s)	1912
AspLEI	GCGC	3 cut(s)	497, 3862, 3889
AspS9I	GGNCC	7 cut(s)	60, 571, 806, 1729, 3644, 3955, 4594
AsuC2I	CCSGG	1 cut(s)	4168
AsuII	TTCGAA	2 cut(s)	1655, 4564
AsuNHI	GCTAGC	1 cut(s)	4418
AvaII	GGWCC	6 cut(s)	571, 806, 1729, 3644, 3955, 4594
BaeGI	GKGCMC	1 cut(s)	4510
BalI	TGGCCA	1 cut(s)	15
BamHI	GGATCC	2 cut(s)	862, 1495
BanI	GGYRCC	2 cut(s)	1358, 4507
BanII	GRGCYC	2 cut(s)	131, 2371
BauI	CACGAG	2 cut(s)	4457, 4673
BbsI	GAAGAC	3 cut(s)	918, 1983, 2766
Bbv12I	GWGCWC	5 cut(s)	131, 1421, 1963, 2371, 2983
BbvCI	CCTCAGC	1 cut(s)	3750
BbvI	GCAGC	7 cut(s)	1119, 1677, 2119, 3437, 3550, 3567, 4620
BccI	CCATC	9 cut(s)	1169, 1777, 2554, 2764, 3311, 3568, 3710, 3818, 3890
BcgI	CGANNNNNNTGC	2 cut(s)	866, 900
BciT130I	CCWGG	7 cut(s)	377, 2694, 3668, 3698, 4140, 4289, 4524
BciVI	GTATCC	4 cut(s)	187, 1945, 3748, 4155
BclI	TGATCA	6 cut(s)	148, 160, 184, 313, 4174, 4387
BcnI	CCSGG	1 cut(s)	4168
BcoDI	GTCTC	8 cut(s)	108, 482, 2104, 2376, 2387, 3186, 3468, 3788
BcuI	ACTAGT	1 cut(s)	1633
BfmI	CTRYAG	5 cut(s)	64, 2154, 2325, 2517, 2964
BfoI	RGCGCY	1 cut(s)	3890
BfrI	CTTAAG	2 cut(s)	1445, 4628
BfuAI	ACCTGC	1 cut(s)	2229
BfuI	GTATCC	4 cut(s)	187, 1945, 3748, 4155
BisI	GCNGC	8 cut(s)	1108, 1691, 2133, 2503, 3426, 3564, 3581, 4634
BlpI	GCTNAGC	1 cut(s)	636
BlsI	GCNGC	8 cut(s)	1109, 1692, 2134, 2504, 3427, 3565, 3582, 4635
BmcAI	AGTACT	1 cut(s)	1847
Bme1390I	CCNGG	8 cut(s)	377, 2694, 3668, 3698, 4140, 4168, 4289, 4524
Bme18I	GGWCC	6 cut(s)	571, 806, 1729, 3644, 3955, 4594
BmeRI	GACNNNNNGTC	1 cut(s)	3976
BmgT120I	GGNCC	7 cut(s)	60, 571, 806, 1729, 3644, 3955, 4594
BmiI	GGNNCC	6 cut(s)	864, 1360, 1497, 2497, 2658, 4509
BmrFI	CCNGG	8 cut(s)	377, 2694, 3668, 3698, 4140, 4168, 4289, 4524
BmrI	ACTGGG	1 cut(s)	4512
BmtI	GCTAGC	1 cut(s)	4422
BmuI	ACTGGG	1 cut(s)	4512
BoxI	GACNNNNGTC	1 cut(s)	1704
BpiI	GAAGAC	3 cut(s)	918, 1983, 2766
BpmI	CTGGAG	5 cut(s)	720, 1325, 1497, 1687, 4506
Bpu10I	CCTNAGC	1 cut(s)	3750
Bpu1102I	GCTNAGC	1 cut(s)	636
Bpu14I	TTCGAA	2 cut(s)	1655, 4564
BpuEI	CTTGAG	3 cut(s)	1447, 2311, 3626
BpuMI	CCSGG	1 cut(s)	4168
Bsa29I	ATCGAT	1 cut(s)	2341
BsaBI	GATNNNNATC	1 cut(s)	546
BsaI	GGTCTC	1 cut(s)	3788
BsaJI	CCNNGG	9 cut(s)	1378, 1947, 2401, 3666, 3697, 3797, 3958, 4139, 4405
BsaWI	WCCGGW	3 cut(s)	308, 1061, 2100
BsaXI	ACNNNNNCTCC	4 cut(s)	650, 680, 1945, 1975
Bse118I	RCCGGY	2 cut(s)	46, 2100
Bse1I	ACTGG	8 cut(s)	1670, 1704, 2590, 3254, 3640, 3763, 3976, 4507
Bse3DI	GCAATG	5 cut(s)	151, 531, 647, 1180, 2806
Bse8I	GATNNNNATC	1 cut(s)	546
BseBI	CCWGG	7 cut(s)	377, 2694, 3668, 3698, 4140, 4289, 4524
BseCI	ATCGAT	1 cut(s)	2341
BseDI	CCNNGG	9 cut(s)	1378, 1947, 2401, 3666, 3697, 3797, 3958, 4139, 4405
BseJI	GATNNNNATC	1 cut(s)	546
BseMI	GCAATG	5 cut(s)	151, 531, 647, 1180, 2806
BseNI	ACTGG	8 cut(s)	1670, 1704, 2590, 3254, 3640, 3763, 3976, 4507
BseRI	GAGGAG	1 cut(s)	225
BseSI	GKGCMC	1 cut(s)	4510
BseX3I	CGGCCG	1 cut(s)	48
BseXI	GCAGC	7 cut(s)	1119, 1677, 2119, 3437, 3550, 3567, 4620
Bsh1236I	CGCG	1 cut(s)	497
Bsh1285I	CGRYCG	1 cut(s)	51
BshFI	GGCC	5 cut(s)	15, 50, 62, 2476, 3696
BshNI	GGYRCC	2 cut(s)	1358, 4507
BshTI	ACCGGT	1 cut(s)	2100
BshVI	ATCGAT	1 cut(s)	2341
BsiEI	CGRYCG	1 cut(s)	51
BsiHKAI	GWGCWC	5 cut(s)	131, 1421, 1963, 2371, 2983
BsiSI	CCGG	6 cut(s)	47, 309, 1062, 2101, 2654, 4167
BslFI	GGGAC	3 cut(s)	299, 997, 3841
BsmAI	GTCTC	8 cut(s)	108, 482, 2104, 2376, 2387, 3186, 3468, 3788
BsmBI	CGTCTC	2 cut(s)	108, 3468
BsmFI	GGGAC	3 cut(s)	299, 997, 3841
BsmI	GAATGC	3 cut(s)	1456, 2543, 3549
BsnI	GGCC	5 cut(s)	15, 50, 62, 2476, 3696
Bso31I	GGTCTC	1 cut(s)	3788
Bsp119I	TTCGAA	2 cut(s)	1655, 4564
Bsp1286I	GDGCHC	6 cut(s)	131, 1421, 1963, 2371, 2983, 4510
Bsp1407I	TGTACA	2 cut(s)	416, 4054
Bsp1720I	GCTNAGC	1 cut(s)	636
Bsp19I	CCATGG	2 cut(s)	1378, 4405
BspANI	GGCC	5 cut(s)	15, 50, 62, 2476, 3696
BspDI	ATCGAT	1 cut(s)	2341
BspFNI	CGCG	1 cut(s)	497
BspHI	TCATGA	1 cut(s)	3676
BspLI	GGNNCC	6 cut(s)	864, 1360, 1497, 2497, 2658, 4509
BspMI	ACCTGC	1 cut(s)	2229
BspOI	GCTAGC	1 cut(s)	4422
BspQI	GCTCTTC	1 cut(s)	4474
BspT104I	TTCGAA	2 cut(s)	1655, 4564
BspT107I	GGYRCC	2 cut(s)	1358, 4507
BspTI	CTTAAG	2 cut(s)	1445, 4628
BspTNI	GGTCTC	1 cut(s)	3788
BsrDI	GCAATG	5 cut(s)	151, 531, 647, 1180, 2806
BsrFI	RCCGGY	2 cut(s)	46, 2100
BsrGI	TGTACA	2 cut(s)	416, 4054
BsrI	ACTGG	8 cut(s)	1670, 1704, 2590, 3254, 3640, 3763, 3976, 4507
BssAI	RCCGGY	2 cut(s)	46, 2100
BssECI	CCNNGG	9 cut(s)	1378, 1947, 2401, 3666, 3697, 3797, 3958, 4139, 4405
BssNAI	GTATAC	3 cut(s)	2941, 3042, 3051
BssSI	CACGAG	2 cut(s)	4457, 4673
BssT1I	CCWWGG	5 cut(s)	1378, 1947, 2401, 3958, 4405
Bst1107I	GTATAC	3 cut(s)	2941, 3042, 3051
Bst2BI	CACGAG	2 cut(s)	4457, 4673
Bst2UI	CCWGG	7 cut(s)	377, 2694, 3668, 3698, 4140, 4289, 4524
Bst6I	CTCTTC	4 cut(s)	2020, 2385, 4474, 4651
BstAFI	CTTAAG	2 cut(s)	1445, 4628
BstAPI	GCANNNNNTGC	1 cut(s)	590
BstAUI	TGTACA	2 cut(s)	416, 4054
BstBI	TTCGAA	2 cut(s)	1655, 4564
BstC8I	GCNNGC	8 cut(s)	48, 477, 688, 1796, 2557, 3087, 3585, 4420
BstDSI	CCRYGG	3 cut(s)	1378, 3797, 4405
BstEII	GGTNACC	1 cut(s)	2216
BstENI	CCTNNNNNAGG	2 cut(s)	801, 4524
BstFNI	CGCG	1 cut(s)	497
BstH2I	RGCGCY	1 cut(s)	3890
BstHHI	GCGC	3 cut(s)	497, 3862, 3889
BstMAI	GTCTC	8 cut(s)	108, 482, 2104, 2376, 2387, 3186, 3468, 3788
BstMCI	CGRYCG	1 cut(s)	51
BstMWI	GCNNNNNNNGC	7 cut(s)	521, 590, 710, 996, 1383, 1604, 3510
BstNI	CCWGG	7 cut(s)	377, 2694, 3668, 3698, 4140, 4289, 4524
BstNSI	RCATGY	9 cut(s)	690, 1432, 1798, 1835, 1945, 2605, 3073, 3587, 3706
BstPAI	GACNNNNGTC	1 cut(s)	1704
BstPI	GGTNACC	1 cut(s)	2216
BstSCI	CCNGG	8 cut(s)	375, 2692, 3666, 3696, 4138, 4166, 4287, 4522
BstSFI	CTRYAG	5 cut(s)	64, 2154, 2325, 2517, 2964
BstSLI	GKGCMC	1 cut(s)	4510
BstUI	CGCG	1 cut(s)	497
BstV1I	GCAGC	7 cut(s)	1119, 1677, 2119, 3437, 3550, 3567, 4620
BstV2I	GAAGAC	3 cut(s)	918, 1983, 2766
BstX2I	RGATCY	6 cut(s)	862, 1495, 2203, 3722, 3910, 4114
BstXI	CCANNNNNNTGG	2 cut(s)	4201, 4261
BstYI	RGATCY	6 cut(s)	862, 1495, 2203, 3722, 3910, 4114
BstZ17I	GTATAC	3 cut(s)	2941, 3042, 3051
BstZI	CGGCCG	1 cut(s)	48
Bsu15I	ATCGAT	1 cut(s)	2341
BsuI	GTATCC	4 cut(s)	187, 1945, 3748, 4155
BsuRI	GGCC	5 cut(s)	15, 50, 62, 2476, 3696
BsuTUI	ATCGAT	1 cut(s)	2341
BtgI	CCRYGG	3 cut(s)	1378, 3797, 4405
BtsI	GCAGTG	2 cut(s)	2346, 4413
BveI	ACCTGC	1 cut(s)	2229
Cac8I	GCNNGC	8 cut(s)	48, 477, 688, 1796, 2557, 3087, 3585, 4420
CaiI	CAGNNNCTG	2 cut(s)	376, 590
CciI	TCATGA	1 cut(s)	3676
CfoI	GCGC	3 cut(s)	497, 3862, 3889
Cfr10I	RCCGGY	2 cut(s)	46, 2100
Cfr13I	GGNCC	7 cut(s)	60, 571, 806, 1729, 3644, 3955, 4594
ClaI	ATCGAT	1 cut(s)	2341
CseI	GACGC	1 cut(s)	4328
CsiI	ACCWGGT	2 cut(s)	375, 4287
Csp6I	GTAC	8 cut(s)	90, 417, 565, 1846, 3599, 3633, 4055, 4642
CspAI	ACCGGT	1 cut(s)	2100
CviQI	GTAC	8 cut(s)	90, 417, 565, 1846, 3599, 3633, 4055, 4642
DrdI	GACNNNNNNGTC	1 cut(s)	2089
DriI	GACNNNNNGTC	1 cut(s)	3976
DseDI	GACNNNNNNGTC	1 cut(s)	2089
EaeI	YGGCCR	2 cut(s)	13, 48
EagI	CGGCCG	1 cut(s)	48
Eam1104I	CTCTTC	4 cut(s)	2020, 2385, 4474, 4651
Eam1105I	GACNNNNNGTC	1 cut(s)	3976
EarI	CTCTTC	4 cut(s)	2020, 2385, 4474, 4651
EciI	GGCGGA	2 cut(s)	313, 4601
Ecl136II	GAGCTC	2 cut(s)	129, 2369
EclXI	CGGCCG	1 cut(s)	48
Eco130I	CCWWGG	5 cut(s)	1378, 1947, 2401, 3958, 4405
Eco24I	GRGCYC	2 cut(s)	131, 2371
Eco31I	GGTCTC	1 cut(s)	3788
Eco32I	GATATC	2 cut(s)	502, 2146
Eco47I	GGWCC	6 cut(s)	571, 806, 1729, 3644, 3955, 4594
Eco47III	AGCGCT	1 cut(s)	3888
Eco52I	CGGCCG	1 cut(s)	48
Eco53kI	GAGCTC	2 cut(s)	129, 2369
Eco57I	CTGAAG	8 cut(s)	822, 1063, 1226, 1880, 2090, 3875, 4078, 4344
Eco91I	GGTNACC	1 cut(s)	2216
EcoICRI	GAGCTC	2 cut(s)	129, 2369
EcoNI	CCTNNNNNAGG	2 cut(s)	801, 4524
EcoO109I	RGGNCCY	4 cut(s)	60, 806, 1729, 3955
EcoO65I	GGTNACC	1 cut(s)	2216
EcoRI	GAATTC	1 cut(s)	2064
EcoRII	CCWGG	7 cut(s)	375, 2692, 3666, 3696, 4138, 4287, 4522
EcoRV	GATATC	2 cut(s)	502, 2146
EcoT14I	CCWWGG	5 cut(s)	1378, 1947, 2401, 3958, 4405
EcoT22I	ATGCAT	3 cut(s)	2001, 3235, 3820
EcoT38I	GRGCYC	2 cut(s)	131, 2371
ErhI	CCWWGG	5 cut(s)	1378, 1947, 2401, 3958, 4405
Esp3I	CGTCTC	2 cut(s)	108, 3468
FalI	AAGNNNNNCTT	2 cut(s)	348, 380
FaqI	GGGAC	3 cut(s)	299, 997, 3841
FauI	CCCGC	1 cut(s)	3306
FauNDI	CATATG	1 cut(s)	189
FbaI	TGATCA	6 cut(s)	148, 160, 184, 313, 4174, 4387
FblI	GTMKAC	5 cut(s)	646, 2940, 3041, 3050, 4342
Fnu4HI	GCNGC	8 cut(s)	1108, 1691, 2133, 2503, 3426, 3564, 3581, 4634
FriOI	GRGCYC	2 cut(s)	131, 2371
Fsp4HI	GCNGC	8 cut(s)	1108, 1691, 2133, 2503, 3426, 3564, 3581, 4634
GlaI	GCGC	3 cut(s)	496, 3861, 3888
GluI	GCNGC	8 cut(s)	1108, 1691, 2133, 2503, 3426, 3564, 3581, 4634
GsuI	CTGGAG	5 cut(s)	720, 1325, 1497, 1687, 4506
HaeII	RGCGCY	1 cut(s)	3890
HaeIII	GGCC	5 cut(s)	15, 50, 62, 2476, 3696
HapII	CCGG	6 cut(s)	47, 309, 1062, 2101, 2654, 4167
HgaI	GACGC	1 cut(s)	4328
HhaI	GCGC	3 cut(s)	497, 3862, 3889
Hin6I	GCGC	3 cut(s)	495, 3860, 3887
HinP1I	GCGC	3 cut(s)	495, 3860, 3887
HincII	GTYRAC	3 cut(s)	2434, 2998, 4554
HindII	GTYRAC	3 cut(s)	2434, 2998, 4554
HindIII	AAGCTT	4 cut(s)	1901, 2015, 3142, 4266
HpaI	GTTAAC	1 cut(s)	4554
HpaII	CCGG	6 cut(s)	47, 309, 1062, 2101, 2654, 4167
HpyCH4IV	ACGT	3 cut(s)	2864, 3044, 4357
HpyF10VI	GCNNNNNNNGC	7 cut(s)	521, 590, 710, 996, 1383, 1604, 3510
HpySE526I	ACGT	3 cut(s)	2864, 3044, 4357
HspAI	GCGC	3 cut(s)	495, 3860, 3887
KroI	GCCGGC	1 cut(s)	46
KroNI	GCCGGC	1 cut(s)	48
Ksp22I	TGATCA	6 cut(s)	148, 160, 184, 313, 4174, 4387
KspAI	GTTAAC	1 cut(s)	4554
LguI	GCTCTTC	1 cut(s)	4474
LmnI	GCTCC	7 cut(s)	134, 673, 701, 1258, 1682, 1966, 3577
Lsp1109I	GCAGC	7 cut(s)	1119, 1677, 2119, 3437, 3550, 3567, 4620
MabI	ACCWGGT	2 cut(s)	375, 4287
MaeII	ACGT	3 cut(s)	2864, 3044, 4357
MflI	RGATCY	6 cut(s)	862, 1495, 2203, 3722, 3910, 4114
MhlI	GDGCHC	6 cut(s)	131, 1421, 1963, 2371, 2983, 4510
MlsI	TGGCCA	1 cut(s)	15
MluNI	TGGCCA	1 cut(s)	15
MlyI	GAGTC	7 cut(s)	928, 1488, 1562, 1694, 2993, 3781, 4088
MmeI	TCCRAC	3 cut(s)	598, 3090, 3671
Mox20I	TGGCCA	1 cut(s)	15
Mph1103I	ATGCAT	3 cut(s)	2001, 3235, 3820
MroNI	GCCGGC	1 cut(s)	46
MroXI	GAANNNNTTC	1 cut(s)	1912
MscI	TGGCCA	1 cut(s)	15
MslI	CAYNNNNRTG	3 cut(s)	685, 2356, 2768
Msp20I	TGGCCA	1 cut(s)	15
MspA1I	CMGCKG	1 cut(s)	2848
MspCI	CTTAAG	2 cut(s)	1445, 4628
MspI	CCGG	6 cut(s)	47, 309, 1062, 2101, 2654, 4167
MspR9I	CCNGG	8 cut(s)	377, 2694, 3668, 3698, 4140, 4168, 4289, 4524
Mva1269I	GAATGC	3 cut(s)	1456, 2543, 3549
MvaI	CCWGG	7 cut(s)	377, 2694, 3668, 3698, 4140, 4289, 4524
MvnI	CGCG	1 cut(s)	497
MwoI	GCNNNNNNNGC	7 cut(s)	521, 590, 710, 996, 1383, 1604, 3510
NaeI	GCCGGC	1 cut(s)	48
NciI	CCSGG	1 cut(s)	4168
NcoI	CCATGG	2 cut(s)	1378, 4405
NdeI	CATATG	1 cut(s)	189
NgoMIV	GCCGGC	1 cut(s)	46
NheI	GCTAGC	1 cut(s)	4418
NlaIV	GGNNCC	6 cut(s)	864, 1360, 1497, 2497, 2658, 4509
NmeAIII	GCCGAG	2 cut(s)	2981, 4562
NmuCI	GTSAC	6 cut(s)	456, 666, 1865, 2216, 3488, 4498
NsiI	ATGCAT	3 cut(s)	2001, 3235, 3820
NspI	RCATGY	9 cut(s)	690, 1432, 1798, 1835, 1945, 2605, 3073, 3587, 3706
NspV	TTCGAA	2 cut(s)	1655, 4564
PaeI	GCATGC	3 cut(s)	690, 1798, 3587
PagI	TCATGA	1 cut(s)	3676
PciI	ACATGT	2 cut(s)	1941, 3069
PciSI	GCTCTTC	1 cut(s)	4474
PcsI	WCGNNNNNNNCGW	2 cut(s)	458, 2204
PctI	GAATGC	3 cut(s)	1456, 2543, 3549
PdiI	GCCGGC	1 cut(s)	48
PdmI	GAANNNNTTC	1 cut(s)	1912
PflMI	CCANNNNNTGG	3 cut(s)	2585, 2678, 3574
PfoI	TCCNGGA	2 cut(s)	2692, 4522
PinAI	ACCGGT	1 cut(s)	2100
PkrI	GCNGC	8 cut(s)	1109, 1692, 2134, 2504, 3427, 3565, 3582, 4635
PleI	GAGTC	7 cut(s)	927, 1487, 1562, 1694, 2993, 3780, 4087
PpsI	GAGTC	7 cut(s)	927, 1487, 1562, 1694, 2993, 3780, 4087
PpuMI	RGGWCCY	3 cut(s)	806, 1729, 3955
PscI	ACATGT	2 cut(s)	1941, 3069
PshAI	GACNNNNGTC	1 cut(s)	1704
PshBI	ATTAAT	1 cut(s)	3365
PsiI	TTATAA	1 cut(s)	3936
Psp124BI	GAGCTC	2 cut(s)	131, 2371
Psp5II	RGGWCCY	3 cut(s)	806, 1729, 3955
Psp6I	CCWGG	7 cut(s)	375, 2692, 3666, 3696, 4138, 4287, 4522
PspEI	GGTNACC	1 cut(s)	2216
PspGI	CCWGG	7 cut(s)	375, 2692, 3666, 3696, 4138, 4287, 4522
PspN4I	GGNNCC	6 cut(s)	864, 1360, 1497, 2497, 2658, 4509
PspPI	GGNCC	7 cut(s)	60, 571, 806, 1729, 3644, 3955, 4594
PspPPI	RGGWCCY	3 cut(s)	806, 1729, 3955
PsrI	GAACNNNNNNTAC	2 cut(s)	1185, 1217
PstNI	CAGNNNCTG	2 cut(s)	376, 590
PsuI	RGATCY	6 cut(s)	862, 1495, 2203, 3722, 3910, 4114
PvuII	CAGCTG	1 cut(s)	2848
RsaI	GTAC	8 cut(s)	91, 418, 566, 1847, 3600, 3634, 4056, 4643
RsaNI	GTAC	8 cut(s)	90, 417, 565, 1846, 3599, 3633, 4055, 4642
RseI	CAYNNNNRTG	3 cut(s)	685, 2356, 2768
SacI	GAGCTC	2 cut(s)	131, 2371
SapI	GCTCTTC	1 cut(s)	4474
SatI	GCNGC	8 cut(s)	1108, 1691, 2133, 2503, 3426, 3564, 3581, 4634
Sau96I	GGNCC	7 cut(s)	60, 571, 806, 1729, 3644, 3955, 4594
ScaI	AGTACT	1 cut(s)	1847
SchI	GAGTC	7 cut(s)	928, 1488, 1562, 1694, 2993, 3781, 4088
ScrFI	CCNGG	8 cut(s)	377, 2694, 3668, 3698, 4140, 4168, 4289, 4524
SduI	GDGCHC	6 cut(s)	131, 1421, 1963, 2371, 2983, 4510
SexAI	ACCWGGT	2 cut(s)	375, 4287
SfcI	CTRYAG	5 cut(s)	64, 2154, 2325, 2517, 2964
SfuI	TTCGAA	2 cut(s)	1655, 4564
SinI	GGWCC	6 cut(s)	571, 806, 1729, 3644, 3955, 4594
SmiMI	CAYNNNNRTG	3 cut(s)	685, 2356, 2768
SmlI	CTYRAG	5 cut(s)	1445, 1462, 2290, 3605, 4628
SmoI	CTYRAG	5 cut(s)	1445, 1462, 2290, 3605, 4628
SpeI	ACTAGT	1 cut(s)	1633
SphI	GCATGC	3 cut(s)	690, 1798, 3587
SstI	GAGCTC	2 cut(s)	131, 2371
StyD4I	CCNGG	8 cut(s)	375, 2692, 3666, 3696, 4138, 4166, 4287, 4522
StyI	CCWWGG	5 cut(s)	1378, 1947, 2401, 3958, 4405
TaiI	ACGT	3 cut(s)	2867, 3047, 4360
TaqI	TCGA	8 cut(s)	360, 446, 876, 1489, 1655, 2341, 3520, 4564
TaqII	GACCGA	2 cut(s)	100, 3305
TatI	WGTACW	5 cut(s)	89, 416, 564, 1845, 4054
TauI	GCSGC	1 cut(s)	2505
TseFI	GTSAC	6 cut(s)	456, 666, 1865, 2216, 3488, 4498
TseI	GCWGC	7 cut(s)	1107, 1690, 2132, 3425, 3563, 3580, 4633
Tsp45I	GTSAC	6 cut(s)	456, 666, 1865, 2216, 3488, 4498
TspGWI	ACGGA	7 cut(s)	1320, 1537, 2196, 2257, 2322, 3322, 3786
Van91I	CCANNNNNTGG	3 cut(s)	2585, 2678, 3574
Vha464I	CTTAAG	2 cut(s)	1445, 4628
VpaK11BI	GGWCC	6 cut(s)	571, 806, 1729, 3644, 3955, 4594
VspI	ATTAAT	1 cut(s)	3365
XagI	CCTNNNNNAGG	2 cut(s)	801, 4524
XceI	RCATGY	9 cut(s)	690, 1432, 1798, 1835, 1945, 2605, 3073, 3587, 3706
XmiI	GTMKAC	5 cut(s)	646, 2940, 3041, 3050, 4342
XmnI	GAANNNNTTC	1 cut(s)	1912
ZrmI	AGTACT	1 cut(s)	1847
Zsp2I	ATGCAT	3 cut(s)	2001, 3235, 3820

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.