Rw3G013670

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0011401
Physical Location & Seq
Forward (+)
13510408 .. 13516882
6475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G013670.1

Sequence Viewer

Length: 4623 bp
ATGGAAAGTCGTGGCCAAAAGCGGTCTGAGATTGATGATGAATTGCCGGCCGATAAGAGGGCCTGTAGTTCTTTGGAGTTTAGACCGAGTACATCCAGCTCGTCTCAAACCCCTATCAATTCTGTGAGCTCCACGCCCGAACGCAATGATCAGAACAATCATGATCATGCTGATCATATGGATACTGAATCCGAAGGAGGAGAGCCTGAGAAGGATTCGGCGTATGATTCTTGTGATGAGGATGAGGAAGATCAAAGACACAGTGATCTTAGGGACTATCAGAGGCGGAGAGCATCCGGTGATCATGGAAGGTTTCAGACCATAATATCATCTCTGAGTGAAGAAGTCGATCTTTCTCAGCACCTGGTTATGCTCACCGAGTTATGCGAAGTGTTATCTTTTTGTACAGAGGATTCACTGTCAGGCGATACATCGAACTCGTTGTCACCGATTCTTGTTAAGCTGTCAAGGGATGAGACTAGTGCGGATATCATGCTATTGGCTATAAGGGCAATGACTTATTTATGTGATGTATATCCGAAATCATCAGTGTACTTGGTCCGACATGATGCAGTTCCTGCTCTTTGCCAAAGACTTTTGGCTATTGAGTATTTGGATGTAGCTGAGCAATGTCTACAAGCATTGGAGAAGTTGTCACGGGAGCAACCACTTGCATGCTTACAGGCTGGAGCAATAATGGCTGTTCTGAATTATATAGACTTCTTCTCTACAAGCATACAGAGAGTTGCCCTTTCTACTGTGGTGAACATTTGTAAGAAACTTCCTTCTGAAGGTCCTTCACCTTTCATGGATGCTGTTCCTACATTGTGCAACCTTCTTCAGTATGAGGATCCACAGCTTGTCGAAAATGTTGCTATTTGCTTGATTAGAATTACAGAGCGAGTGAGTCAGTCTTCTGAGATGCTGGATGAACTTTGTAAGCATGGGCTGATTCGTCAAGCCACACATTTTATGAGCTTAAATAGCCGAACTACTCTGTCCCAACCAATTCATAATGGTTTGATAGGGCTACTTGTCAAACTTTCTTCCGGTTCAGTTGTAGCCTTCAGGACTCTATATGAGCTTAATATAAGCAGCATATTAAAGGATATATTATCCACTTATGACCTCTCACATGGAATGTCCTCTACTCATGTTGTGGATGGGCATTGCTATCAGGATCAAGATGCTCCACAGTTGTCAGAGAAAGAATCTTTTTTAATAAATCGGCCTGAGCTTCTGGAAAAGTTTGGGAGGGATATACTTCCGTCTCTGATACAGGTTTGGCTTCTGATATTCATCAACAAGTTAATTTATTTGAGCACATCTGACATGCTCGTTGAACTGCTTAAGAATGCCAACATTTCAAGTTTTCTGGCTGGAGTCTTTACTCGAAAGGATCCTCATGTGTTGGTATCAACCCTACGGATTGCTGAGTTAATCCTGCAAAAGTTCTCTGATAATTTCTTGGACTCATTTATTAAAGAAGGTGTCTTTTTTGCCATTGATGCTTTGCTAACCCCTGAAAAAAGCTCACTAGTAACACTGAACAAATGTTCGAAGCTGGTGTTTCCAGTGTCTAGTGGAACAAGGCTGCTGTCTGACTCTAGTCAGAAGTCTGCTTCAAAAGAGGTCCTGAGATGCTTATGCTATGCTTTTCCTAGCAGTTCCCCATCAGGGTCAGAAAAAGGAAGTTGCATGCTTGAAAAGGATTCTGTTTATAGTCTTGCAAAGCATGTTAGAACCAAGTACTTTGCACCAGAATTATGTGACCCTGATAAATCATTAACTGATGTTCTTCAGAAGCTTAGAACTTTTTCTGCTTCATTAAGTGATTTGATGAACATGTCCTTGGGTACTTGTGCTCCTAATCAGCATGAAGAGAGCTTCTATGGTATAATGCATCAAGTAATGGAAAAGCTTGGCGGAAGAGAACCCATTTCCACTTTTGAATTGATTGAAAGTGGAATTCTGAAGTCCTTGATGACTTACTTGTCCAATGACCGGTATTTGAGACAGAAGGGTGATCTTGTAGCTGCTAAAGGTGATATCTATGCTGTAGAAAAAAGATTTGAGGTGTTCGCAAGGCTATTATTTTCTTCGCCAGATCCGTTCTCTGGTGACCTGCCCATTGTAACATTGATACGGAGATTACAAAGCTCACTATCTACTTTGGAAAATTTCCCCGTTATCTTGAGCCATATACCCAAGCAGAGGAACTCATATGCTACAGTTCCATATGAGCGATACACTGCATATCCATGTATGAGAGCTCGTTTTGTTAGAGACAAGGAAGAGACTACCCTTGGTGATTGCTGTGAAGATGTTTTGACCGTTGACCCTTTTTCTTCATTGGATGCCATTGAAGGATATGTGTGGCCTAAAGTCAATGCAAAAGGAACCAGCTGCATCAAATCAACTACAATTGAGGGTCAATCAGAATGCACACTATCAAATGCCAGCTCTAGTCAAGGAGGGTGTCCAAATACTGGTGAGCCTGAGAGCATGTCGGCAGACTTGCCTGAGTTGAAGGCAGATGAGGTCAATTTAACACAGCCGGAACCTGAACAAGAACCAAGTTTTGGACAGACAAATCCTGGAACATCTTTAGATGAAACTTATACTGATACTGTGGAGAAAGAAGTAGAAGCTCAATCTGAGGAAGACACACAGATGGAAGAACAGTGTCCTTTATCTTGTAGCAATGAAGATGCTTCACCGAAATTGTTGTTTTACCTTGAAGGGAAACAGCTGGAACGGTCATTGACATTGTATCAGGCAATTCTTCAACAACAAATGAAAGAACATGAAATTGTCATTGGTTCAAAACTGTGGAGTAAAGTATACACACTGACATATAGAAAAGCTGTAGGTCAAGAAGGTGCTCATAATGAAGGTGTTGACTCGGCAGAGAGTTCCACTGTATCAGATAAAGTTGGGGTGTATACGTTGTATACTTCACTTTTCTCCAACATGTTTCCTTGTGAGCTTGCTTCTGACTTGGAGAAGTCGAATCCTACCTATGACATTGTATATCTGCTCAAAAGCTTGGAAAGTATGAACAAGTTTATTTTTCATCTGATGTCTCGTCAAAGAATATGTGCTTTTGCCGAAGGGAAAATCAATGACTTGGATGCATTTCAGATAGCAGTTACACCAGTGCCACAGAATGAGTTTTTGAGCAGTAAACTGACCGAAAAACTAGAACAGCAGATGCGGGATGGTTTGGCTGTGTCCGTTGGTGGTATGCCCCTGTGGTGTAATCAATTAATGGCATCGTGTCCTTTTTTGTTTAGTTTTGAGGTCAAATGCAAGTATTTCCGCTTGGCAGCCTTTGTTCCACTACTAGGTCAGCCTCCTTCACCGTCTCACAGTGATTCAGGTATGACGAGTGACAGGCGACAAAGCTCTGGTGGCTTACCTCGACAAAAGTTTTTAGTTTTCCGCAACCGCATTCTGGATTCTGCTGCCCAAATGATGGAGCTGCATGCCTGTCAGAAGGTACTTCTTGAGGTGGAATATGATGAAGAAGTTGGTACTGGTCTTGGTCCAACATTGGAATTTTATACCCTGGTTAGTCATGAGTTTCAGAAATCTGGCCTGGGCATGTGGAGAGAGGATGGTGGATCTTTTACCTCTGGTACAAGCCATGCTGAGGATACTGGAATTTTGATAAGTCCTTGTGGTCTCTTTCCTCGTCCGTGGTCATCTACTATGGATGCATCAGATGGGACACAGTTCAGTGAAGTAATCAAGAAGTTTGCGCTTTTGGGGAAAATTGTAGGGAAAGCGCTTCAGGATGGGAGGGTCTTGGATCTGCATTTCTCCAAAGCCTTTTATAAACTAATTCTTGGACAGGACCTTGGTCTGTATGACATCCAGTCATTTGATCCTGTGCTTGGAAGGACACTACTAGAGTTCAAGGCTCTTGTGGAAAGGAAAAGGTTTTTGGAATCTGTACATGGAGAAAATACAACATTTGAGTTTGACTCGTGCTTCAGGGAAACTAGAATTGAGGATCTTTGCCTTGATTTTACACTTCCTGGGTATCCTGATTTTGTCCTTGCATCCGGGTTTGATCATAAAATGGTAAATTCCACGAACTTGGAAGATTATGTTTCACTTATGGCAGATGCAACTATAAATTCTGGAATTTCCAGACAAGTGGAAGCTTTCAAGTCTGGTTTTAACCAGGTGTTCCCTATTGAGCATCTTCAGATTTTCACTGAAGAAGAACTGGAGCGTCTACTATGTGGAGAACGTGATTCTTGGGCTTTCAATGAGCTCCTTGATCATATCAAATTTGACCATGGTTACACTGCTAGCAGTCCTCCCATTGTTAATTTGCTGGAAATCATACATGAGTTTGACCAAGAACAGCGAAGAGCGTTTCTGCAATTTGTGACTGGGGCACCTCGCTTACCCCCAGGGGGTTTTGCATCCCTCAATCCGAAGTTAACTATTGTTCGAAAGCACTCTAGCAACTCGGCGGACTTGGACCTACCAAGTGTGATGACTTGTGCAAACTATCTTAAGCTGCCACCATACTCTTTGAAAGAGATGATGAAGGAGAAGCTCGTGTATGCCATTAAAGAAGGGCAGGGATCATTCCACCTTTCATAG

Protein Analysis

1540

Amino Acids

172.07

Weight (kDa)

4.98

Isoelectric Point (pI)

52.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_TRIP12_N PF25579 4 - 228 1.1e-102 E3 ubiquitin-protein ligase TRIP12-like, TPR repeats
HECT PF00632 1192 - 1540 3.6e-81 HECT-domain (ubiquitin-transferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011401)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02880 AT5G02880
fragaria_vesca FvH4_6g15270 FvH4_6g15270 FvH4_6g15270 FvH4_6g15270
malus_domestica MD04G1104600.v1.1 MD12G1124500.v1.1
prunus_persica Prupe.6G241700_v2.0.a1 Prupe.6G241700_v2.0.a1
pyrus_communis pycom12g12310
rosa_chinensis RchiOBHm_Chr3g0468671
rosa_laevigata RLG00000024416
rosa_multiflora Rmu_sc0011538.1_g000007
rosa_roxburghii Rroxscaffold_6G00412660
rosa_rugosa Rorug03G0096200
rosa_samantha Rh3AG145000 Rh3BG167500 Rh3DG166300
rosa_wichuraiana Rw3G013670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3840
AasI GACNNNNNNGTC 1 cut(s) 1993
Acc36I ACCTGC 1 cut(s) 2133
AccB1I GGYRCC 1 cut(s) 4411
AccB7I CCANNNNNTGG 3 cut(s) 2489, 2582, 3478
AccI GTMKAC 5 cut(s) 634, 2844, 2945, 2954, 4246
AciI CCGC 9 cut(s) 22, 286, 485, 1926, 3217, 3322, 3445, 3451, 4490
AcoI YGGCCR 2 cut(s) 13, 48
AcsI RAATTY 8 cut(s) 1968, 2179, 3560, 3666, 4093, 4144, 4152, 4301
AcuI CTGAAG 9 cut(s) 810, 824, 1051, 1784, 1994, 3779, 3982, 4199, 4248
AfaI GTAC 9 cut(s) 91, 406, 554, 1751, 1858, 3504, 3538, 3643, 3960
AfeI AGCGCT 1 cut(s) 3792
AflII CTTAAG 2 cut(s) 1349, 4532
AflIII ACRYGT 2 cut(s) 1845, 2973
AgeI ACCGGT 1 cut(s) 2004
AhdI GACNNNNNGTC 1 cut(s) 3880
AhlI ACTAGT 2 cut(s) 479, 1537
AjnI CCWGG 7 cut(s) 363, 2596, 3570, 3600, 4042, 4191, 4426
AjuI GAANNNNNNNTTGG 8 cut(s) 1835, 1867, 2565, 2597, 2802, 2834, 3932, 3964
Alw21I GWGCWC 6 cut(s) 131, 1325, 1867, 2275, 2887, 4287
Alw26I GTCTC 9 cut(s) 108, 470, 1275, 2008, 2280, 2291, 3090, 3372, 3692
AlwNI CAGNNNCTG 2 cut(s) 364, 578
Aor51HI AGCGCT 1 cut(s) 3792
AoxI GGCC 6 cut(s) 13, 48, 60, 1229, 2378, 3598
ApeKI GCWGC 8 cut(s) 1095, 1594, 2036, 2406, 3329, 3467, 3484, 4537
ApoI RAATTY 8 cut(s) 1968, 2179, 3560, 3666, 4093, 4144, 4152, 4301
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
AseI ATTAAT 1 cut(s) 3269
AsiGI ACCGGT 1 cut(s) 2004
Asp700I GAANNNNTTC 1 cut(s) 1816
AspLEI GCGC 2 cut(s) 3766, 3793
AspS9I GGNCC 7 cut(s) 60, 559, 794, 1633, 3548, 3859, 4498
AsuC2I CCSGG 1 cut(s) 4072
AsuII TTCGAA 2 cut(s) 1559, 4468
AsuNHI GCTAGC 1 cut(s) 4322
AvaII GGWCC 6 cut(s) 559, 794, 1633, 3548, 3859, 4498
BaeGI GKGCMC 1 cut(s) 4414
BalI TGGCCA 1 cut(s) 15
BamHI GGATCC 2 cut(s) 850, 1399
BanI GGYRCC 1 cut(s) 4411
BanII GRGCYC 3 cut(s) 131, 2275, 4287
BauI CACGAG 2 cut(s) 3991, 4577
BbsI GAAGAC 2 cut(s) 906, 2670
Bbv12I GWGCWC 6 cut(s) 131, 1325, 1867, 2275, 2887, 4287
BbvCI CCTCAGC 1 cut(s) 3654
BbvI GCAGC 8 cut(s) 1107, 1581, 2023, 2393, 3341, 3454, 3471, 4524
BccI CCATC 8 cut(s) 1157, 1681, 2668, 3215, 3472, 3614, 3722, 3794
BcgI CGANNNNNNTGC 2 cut(s) 854, 888
BciT130I CCWGG 7 cut(s) 365, 2598, 3572, 3602, 4044, 4193, 4428
BciVI GTATCC 3 cut(s) 175, 3652, 4059
BclI TGATCA 6 cut(s) 148, 163, 172, 301, 4078, 4291
BcnI CCSGG 1 cut(s) 4072
BcoDI GTCTC 9 cut(s) 108, 470, 1275, 2008, 2280, 2291, 3090, 3372, 3692
BcuI ACTAGT 2 cut(s) 479, 1537
BfmI CTRYAG 4 cut(s) 64, 2058, 2229, 2868
BfoI RGCGCY 1 cut(s) 3794
BfrI CTTAAG 2 cut(s) 1349, 4532
BfuAI ACCTGC 1 cut(s) 2133
BfuI GTATCC 3 cut(s) 175, 3652, 4059
BisI GCNGC 8 cut(s) 1096, 1595, 2037, 2407, 3330, 3468, 3485, 4538
BlpI GCTNAGC 1 cut(s) 624
BlsI GCNGC 8 cut(s) 1097, 1596, 2038, 2408, 3331, 3469, 3486, 4539
BmcAI AGTACT 1 cut(s) 1751
Bme1390I CCNGG 8 cut(s) 365, 2598, 3572, 3602, 4044, 4072, 4193, 4428
Bme18I GGWCC 6 cut(s) 559, 794, 1633, 3548, 3859, 4498
BmeRI GACNNNNNGTC 1 cut(s) 3880
BmgT120I GGNCC 7 cut(s) 60, 559, 794, 1633, 3548, 3859, 4498
BmiI GGNNCC 5 cut(s) 852, 1401, 2401, 2562, 4413
BmrFI CCNGG 8 cut(s) 365, 2598, 3572, 3602, 4044, 4072, 4193, 4428
BmrI ACTGGG 1 cut(s) 4416
BmtI GCTAGC 1 cut(s) 4326
BmuI ACTGGG 1 cut(s) 4416
BoxI GACNNNNGTC 2 cut(s) 1608, 3864
BpiI GAAGAC 2 cut(s) 906, 2670
BplI GAGNNNNNCTC 2 cut(s) 3974, 4006
BpmI CTGGAG 3 cut(s) 708, 1401, 4259
Bpu10I CCTNAGC 2 cut(s) 1233, 3654
Bpu1102I GCTNAGC 1 cut(s) 624
Bpu14I TTCGAA 2 cut(s) 1559, 4468
BpuEI CTTGAG 2 cut(s) 2215, 3530
BpuMI CCSGG 1 cut(s) 4072
BsaBI GATNNNNATC 2 cut(s) 534, 1298
BsaI GGTCTC 1 cut(s) 3692
BsaWI WCCGGW 3 cut(s) 296, 1049, 2004
BsaXI ACNNNNNCTCC 4 cut(s) 638, 668, 1849, 1879
Bse118I RCCGGY 2 cut(s) 46, 2004
Bse1I ACTGG 8 cut(s) 1574, 2494, 3158, 3544, 3667, 3880, 4242, 4411
Bse3DI GCAATG 5 cut(s) 151, 519, 635, 1168, 2710
Bse8I GATNNNNATC 2 cut(s) 534, 1298
BseBI CCWGG 7 cut(s) 365, 2598, 3572, 3602, 4044, 4193, 4428
BseJI GATNNNNATC 2 cut(s) 534, 1298
BseMI GCAATG 5 cut(s) 151, 519, 635, 1168, 2710
BseNI ACTGG 8 cut(s) 1574, 2494, 3158, 3544, 3667, 3880, 4242, 4411
BseRI GAGGAG 1 cut(s) 213
BseSI GKGCMC 1 cut(s) 4414
BseX3I CGGCCG 1 cut(s) 48
BseXI GCAGC 8 cut(s) 1107, 1581, 2023, 2393, 3341, 3454, 3471, 4524
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 6 cut(s) 15, 50, 62, 1231, 2380, 3600
BshNI GGYRCC 1 cut(s) 4411
BshTI ACCGGT 1 cut(s) 2004
BsiEI CGRYCG 1 cut(s) 51
BsiHKAI GWGCWC 6 cut(s) 131, 1325, 1867, 2275, 2887, 4287
BsiSI CCGG 6 cut(s) 47, 297, 1050, 2005, 2558, 4071
BslFI GGGAC 3 cut(s) 287, 985, 3745
BsmAI GTCTC 9 cut(s) 108, 470, 1275, 2008, 2280, 2291, 3090, 3372, 3692
BsmBI CGTCTC 3 cut(s) 108, 1275, 3372
BsmFI GGGAC 3 cut(s) 287, 985, 3745
BsmI GAATGC 3 cut(s) 1360, 2447, 3453
BsnI GGCC 6 cut(s) 15, 50, 62, 1231, 2380, 3600
Bso31I GGTCTC 1 cut(s) 3692
Bsp119I TTCGAA 2 cut(s) 1559, 4468
Bsp1286I GDGCHC 7 cut(s) 131, 1325, 1867, 2275, 2887, 4287, 4414
Bsp1407I TGTACA 2 cut(s) 404, 3958
Bsp1720I GCTNAGC 1 cut(s) 624
Bsp19I CCATGG 1 cut(s) 4309
BspACI CCGC 9 cut(s) 22, 286, 485, 1926, 3217, 3322, 3445, 3451, 4490
BspANI GGCC 6 cut(s) 15, 50, 62, 1231, 2380, 3600
BspHI TCATGA 2 cut(s) 160, 3580
BspLI GGNNCC 5 cut(s) 852, 1401, 2401, 2562, 4413
BspMI ACCTGC 1 cut(s) 2133
BspOI GCTAGC 1 cut(s) 4326
BspQI GCTCTTC 1 cut(s) 4378
BspT104I TTCGAA 2 cut(s) 1559, 4468
BspT107I GGYRCC 1 cut(s) 4411
BspTI CTTAAG 2 cut(s) 1349, 4532
BspTNI GGTCTC 1 cut(s) 3692
BsrDI GCAATG 5 cut(s) 151, 519, 635, 1168, 2710
BsrFI RCCGGY 2 cut(s) 46, 2004
BsrGI TGTACA 2 cut(s) 404, 3958
BsrI ACTGG 8 cut(s) 1574, 2494, 3158, 3544, 3667, 3880, 4242, 4411
BssAI RCCGGY 2 cut(s) 46, 2004
BssNAI GTATAC 3 cut(s) 2845, 2946, 2955
BssSI CACGAG 2 cut(s) 3991, 4577
BssT1I CCWWGG 4 cut(s) 1851, 2305, 3862, 4309
Bst1107I GTATAC 3 cut(s) 2845, 2946, 2955
Bst2BI CACGAG 2 cut(s) 3991, 4577
Bst2UI CCWGG 7 cut(s) 365, 2598, 3572, 3602, 4044, 4193, 4428
Bst6I CTCTTC 4 cut(s) 1875, 1924, 2289, 4378
BstAFI CTTAAG 2 cut(s) 1349, 4532
BstAPI GCANNNNNTGC 1 cut(s) 578
BstAUI TGTACA 2 cut(s) 404, 3958
BstBI TTCGAA 2 cut(s) 1559, 4468
BstC8I GCNNGC 7 cut(s) 48, 676, 1700, 2461, 2991, 3489, 4324
BstDSI CCRYGG 2 cut(s) 3701, 4309
BstEII GGTNACC 1 cut(s) 2120
BstENI CCTNNNNNAGG 1 cut(s) 789
BstH2I RGCGCY 1 cut(s) 3794
BstHHI GCGC 2 cut(s) 3766, 3793
BstMAI GTCTC 9 cut(s) 108, 470, 1275, 2008, 2280, 2291, 3090, 3372, 3692
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 6 cut(s) 509, 578, 698, 984, 1508, 3414
BstNI CCWGG 7 cut(s) 365, 2598, 3572, 3602, 4044, 4193, 4428
BstNSI RCATGY 9 cut(s) 678, 1336, 1702, 1739, 1849, 2509, 2977, 3491, 3610
BstPAI GACNNNNGTC 2 cut(s) 1608, 3864
BstPI GGTNACC 1 cut(s) 2120
BstSCI CCNGG 8 cut(s) 363, 2596, 3570, 3600, 4042, 4070, 4191, 4426
BstSFI CTRYAG 4 cut(s) 64, 2058, 2229, 2868
BstSLI GKGCMC 1 cut(s) 4414
BstV1I GCAGC 8 cut(s) 1107, 1581, 2023, 2393, 3341, 3454, 3471, 4524
BstV2I GAAGAC 2 cut(s) 906, 2670
BstX2I RGATCY 6 cut(s) 850, 1399, 2107, 3626, 3814, 4018
BstXI CCANNNNNNTGG 2 cut(s) 4105, 4165
BstYI RGATCY 6 cut(s) 850, 1399, 2107, 3626, 3814, 4018
BstZ17I GTATAC 3 cut(s) 2845, 2946, 2955
BstZI CGGCCG 1 cut(s) 48
BsuI GTATCC 3 cut(s) 175, 3652, 4059
BsuRI GGCC 6 cut(s) 15, 50, 62, 1231, 2380, 3600
BtgI CCRYGG 2 cut(s) 3701, 4309
BtsI GCAGTG 2 cut(s) 2250, 4317
BveI ACCTGC 1 cut(s) 2133
Cac8I GCNNGC 7 cut(s) 48, 676, 1700, 2461, 2991, 3489, 4324
CaiI CAGNNNCTG 2 cut(s) 364, 578
CciI TCATGA 2 cut(s) 160, 3580
CfoI GCGC 2 cut(s) 3766, 3793
Cfr10I RCCGGY 2 cut(s) 46, 2004
Cfr13I GGNCC 7 cut(s) 60, 559, 794, 1633, 3548, 3859, 4498
CseI GACGC 1 cut(s) 4232
CsiI ACCWGGT 2 cut(s) 363, 4191
Csp6I GTAC 9 cut(s) 90, 405, 553, 1750, 1857, 3503, 3537, 3642, 3959
CspAI ACCGGT 1 cut(s) 2004
CviQI GTAC 9 cut(s) 90, 405, 553, 1750, 1857, 3503, 3537, 3642, 3959
DrdI GACNNNNNNGTC 1 cut(s) 1993
DriI GACNNNNNGTC 1 cut(s) 3880
DseDI GACNNNNNNGTC 1 cut(s) 1993
EaeI YGGCCR 2 cut(s) 13, 48
EagI CGGCCG 1 cut(s) 48
Eam1104I CTCTTC 4 cut(s) 1875, 1924, 2289, 4378
Eam1105I GACNNNNNGTC 1 cut(s) 3880
EarI CTCTTC 4 cut(s) 1875, 1924, 2289, 4378
EciI GGCGGA 3 cut(s) 301, 1941, 4505
Ecl136II GAGCTC 3 cut(s) 129, 2273, 4285
EclXI CGGCCG 1 cut(s) 48
Eco130I CCWWGG 4 cut(s) 1851, 2305, 3862, 4309
Eco24I GRGCYC 3 cut(s) 131, 2275, 4287
Eco31I GGTCTC 1 cut(s) 3692
Eco32I GATATC 2 cut(s) 490, 2050
Eco47I GGWCC 6 cut(s) 559, 794, 1633, 3548, 3859, 4498
Eco47III AGCGCT 1 cut(s) 3792
Eco52I CGGCCG 1 cut(s) 48
Eco53kI GAGCTC 3 cut(s) 129, 2273, 4285
Eco57I CTGAAG 9 cut(s) 810, 824, 1051, 1784, 1994, 3779, 3982, 4199, 4248
Eco91I GGTNACC 1 cut(s) 2120
EcoICRI GAGCTC 3 cut(s) 129, 2273, 4285
EcoNI CCTNNNNNAGG 1 cut(s) 789
EcoO109I RGGNCCY 4 cut(s) 60, 794, 1633, 3859
EcoO65I GGTNACC 1 cut(s) 2120
EcoRI GAATTC 1 cut(s) 1968
EcoRII CCWGG 7 cut(s) 363, 2596, 3570, 3600, 4042, 4191, 4426
EcoRV GATATC 2 cut(s) 490, 2050
EcoT14I CCWWGG 4 cut(s) 1851, 2305, 3862, 4309
EcoT22I ATGCAT 3 cut(s) 1905, 3139, 3724
EcoT38I GRGCYC 3 cut(s) 131, 2275, 4287
ErhI CCWWGG 4 cut(s) 1851, 2305, 3862, 4309
Esp3I CGTCTC 3 cut(s) 108, 1275, 3372
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FaqI GGGAC 3 cut(s) 287, 985, 3745
FauI CCCGC 1 cut(s) 3210
FauNDI CATATG 3 cut(s) 177, 2224, 2239
FbaI TGATCA 6 cut(s) 148, 163, 172, 301, 4078, 4291
FblI GTMKAC 5 cut(s) 634, 2844, 2945, 2954, 4246
Fnu4HI GCNGC 8 cut(s) 1096, 1595, 2037, 2407, 3330, 3468, 3485, 4538
FriOI GRGCYC 3 cut(s) 131, 2275, 4287
Fsp4HI GCNGC 8 cut(s) 1096, 1595, 2037, 2407, 3330, 3468, 3485, 4538
GlaI GCGC 2 cut(s) 3765, 3792
GluI GCNGC 8 cut(s) 1096, 1595, 2037, 2407, 3330, 3468, 3485, 4538
GsuI CTGGAG 3 cut(s) 708, 1401, 4259
HaeII RGCGCY 1 cut(s) 3794
HaeIII GGCC 6 cut(s) 15, 50, 62, 1231, 2380, 3600
HapII CCGG 6 cut(s) 47, 297, 1050, 2005, 2558, 4071
HgaI GACGC 1 cut(s) 4232
HhaI GCGC 2 cut(s) 3766, 3793
Hin6I GCGC 2 cut(s) 3764, 3791
HinP1I GCGC 2 cut(s) 3764, 3791
HincII GTYRAC 3 cut(s) 2338, 2902, 4458
HindII GTYRAC 3 cut(s) 2338, 2902, 4458
HindIII AAGCTT 4 cut(s) 1805, 1919, 3046, 4170
HpaI GTTAAC 1 cut(s) 4458
HpaII CCGG 6 cut(s) 47, 297, 1050, 2005, 2558, 4071
HpyCH4IV ACGT 2 cut(s) 2948, 4261
HpyF10VI GCNNNNNNNGC 6 cut(s) 509, 578, 698, 984, 1508, 3414
HpySE526I ACGT 2 cut(s) 2948, 4261
HspAI GCGC 2 cut(s) 3764, 3791
KroI GCCGGC 1 cut(s) 46
KroNI GCCGGC 1 cut(s) 48
Ksp22I TGATCA 6 cut(s) 148, 163, 172, 301, 4078, 4291
KspAI GTTAAC 1 cut(s) 4458
LguI GCTCTTC 1 cut(s) 4378
LmnI GCTCC 8 cut(s) 134, 661, 689, 1195, 1870, 3481, 4240, 4290
Lsp1109I GCAGC 8 cut(s) 1107, 1581, 2023, 2393, 3341, 3454, 3471, 4524
MabI ACCWGGT 2 cut(s) 363, 4191
MaeII ACGT 2 cut(s) 2948, 4261
MfeI CAATTG 1 cut(s) 2424
MflI RGATCY 6 cut(s) 850, 1399, 2107, 3626, 3814, 4018
MhlI GDGCHC 7 cut(s) 131, 1325, 1867, 2275, 2887, 4287, 4414
MlsI TGGCCA 1 cut(s) 15
MluNI TGGCCA 1 cut(s) 15
MlyI GAGTC 7 cut(s) 916, 1066, 1392, 1466, 1598, 2897, 3983
MmeI TCCRAC 3 cut(s) 586, 2994, 3575
Mox20I TGGCCA 1 cut(s) 15
Mph1103I ATGCAT 3 cut(s) 1905, 3139, 3724
MroNI GCCGGC 1 cut(s) 46
MroXI GAANNNNTTC 1 cut(s) 1816
MscI TGGCCA 1 cut(s) 15
MslI CAYNNNNRTG 4 cut(s) 165, 673, 2260, 2672
Msp20I TGGCCA 1 cut(s) 15
MspA1I CMGCKG 2 cut(s) 2406, 2752
MspCI CTTAAG 2 cut(s) 1349, 4532
MspI CCGG 6 cut(s) 47, 297, 1050, 2005, 2558, 4071
MspR9I CCNGG 8 cut(s) 365, 2598, 3572, 3602, 4044, 4072, 4193, 4428
MunI CAATTG 1 cut(s) 2424
Mva1269I GAATGC 3 cut(s) 1360, 2447, 3453
MvaI CCWGG 7 cut(s) 365, 2598, 3572, 3602, 4044, 4193, 4428
MwoI GCNNNNNNNGC 6 cut(s) 509, 578, 698, 984, 1508, 3414
NaeI GCCGGC 1 cut(s) 48
NciI CCSGG 1 cut(s) 4072
NcoI CCATGG 1 cut(s) 4309
NdeI CATATG 3 cut(s) 177, 2224, 2239
NgoMIV GCCGGC 1 cut(s) 46
NheI GCTAGC 1 cut(s) 4322
NlaIV GGNNCC 5 cut(s) 852, 1401, 2401, 2562, 4413
NmeAIII GCCGAG 2 cut(s) 2885, 4466
NmuCI GTSAC 6 cut(s) 444, 654, 1769, 2120, 3392, 4402
NsiI ATGCAT 3 cut(s) 1905, 3139, 3724
NspI RCATGY 9 cut(s) 678, 1336, 1702, 1739, 1849, 2509, 2977, 3491, 3610
NspV TTCGAA 2 cut(s) 1559, 4468
PaeI GCATGC 3 cut(s) 678, 1702, 3491
PagI TCATGA 2 cut(s) 160, 3580
PasI CCCWGGG 1 cut(s) 4427
PciI ACATGT 2 cut(s) 1845, 2973
PciSI GCTCTTC 1 cut(s) 4378
PcsI WCGNNNNNNNCGW 2 cut(s) 446, 2108
PctI GAATGC 3 cut(s) 1360, 2447, 3453
PdiI GCCGGC 1 cut(s) 48
PdmI GAANNNNTTC 1 cut(s) 1816
PflMI CCANNNNNTGG 3 cut(s) 2489, 2582, 3478
PfoI TCCNGGA 1 cut(s) 2596
PinAI ACCGGT 1 cut(s) 2004
PkrI GCNGC 8 cut(s) 1097, 1596, 2038, 2408, 3331, 3469, 3486, 4539
PleI GAGTC 7 cut(s) 915, 1066, 1391, 1466, 1598, 2897, 3983
PpsI GAGTC 7 cut(s) 915, 1066, 1391, 1466, 1598, 2897, 3983
PpuMI RGGWCCY 3 cut(s) 794, 1633, 3859
PscI ACATGT 2 cut(s) 1845, 2973
PshAI GACNNNNGTC 2 cut(s) 1608, 3864
PshBI ATTAAT 1 cut(s) 3269
PsiI TTATAA 1 cut(s) 3840
Psp124BI GAGCTC 3 cut(s) 131, 2275, 4287
Psp5II RGGWCCY 3 cut(s) 794, 1633, 3859
Psp6I CCWGG 7 cut(s) 363, 2596, 3570, 3600, 4042, 4191, 4426
PspEI GGTNACC 1 cut(s) 2120
PspGI CCWGG 7 cut(s) 363, 2596, 3570, 3600, 4042, 4191, 4426
PspN4I GGNNCC 5 cut(s) 852, 1401, 2401, 2562, 4413
PspPI GGNCC 7 cut(s) 60, 559, 794, 1633, 3548, 3859, 4498
PspPPI RGGWCCY 3 cut(s) 794, 1633, 3859
PstNI CAGNNNCTG 2 cut(s) 364, 578
PsuI RGATCY 6 cut(s) 850, 1399, 2107, 3626, 3814, 4018
PvuII CAGCTG 2 cut(s) 2406, 2752
RsaI GTAC 9 cut(s) 91, 406, 554, 1751, 1858, 3504, 3538, 3643, 3960
RsaNI GTAC 9 cut(s) 90, 405, 553, 1750, 1857, 3503, 3537, 3642, 3959
RseI CAYNNNNRTG 4 cut(s) 165, 673, 2260, 2672
SacI GAGCTC 3 cut(s) 131, 2275, 4287
SapI GCTCTTC 1 cut(s) 4378
SatI GCNGC 8 cut(s) 1096, 1595, 2037, 2407, 3330, 3468, 3485, 4538
Sau96I GGNCC 7 cut(s) 60, 559, 794, 1633, 3548, 3859, 4498
ScaI AGTACT 1 cut(s) 1751
SchI GAGTC 7 cut(s) 916, 1066, 1392, 1466, 1598, 2897, 3983
ScrFI CCNGG 8 cut(s) 365, 2598, 3572, 3602, 4044, 4072, 4193, 4428
SduI GDGCHC 7 cut(s) 131, 1325, 1867, 2275, 2887, 4287, 4414
SexAI ACCWGGT 2 cut(s) 363, 4191
SfcI CTRYAG 4 cut(s) 64, 2058, 2229, 2868
SfuI TTCGAA 2 cut(s) 1559, 4468
SinI GGWCC 6 cut(s) 559, 794, 1633, 3548, 3859, 4498
SmiMI CAYNNNNRTG 4 cut(s) 165, 673, 2260, 2672
SmlI CTYRAG 4 cut(s) 1349, 2194, 3509, 4532
SmoI CTYRAG 4 cut(s) 1349, 2194, 3509, 4532
SpeI ACTAGT 2 cut(s) 479, 1537
SphI GCATGC 3 cut(s) 678, 1702, 3491
SsiI CCGC 9 cut(s) 22, 286, 485, 1926, 3217, 3322, 3445, 3451, 4490
SstI GAGCTC 3 cut(s) 131, 2275, 4287
StyD4I CCNGG 8 cut(s) 363, 2596, 3570, 3600, 4042, 4070, 4191, 4426
StyI CCWWGG 4 cut(s) 1851, 2305, 3862, 4309
TaiI ACGT 2 cut(s) 2951, 4264
TaqI TCGA 8 cut(s) 348, 434, 864, 1393, 1559, 3011, 3424, 4468
TaqII GACCGA 2 cut(s) 100, 3209
TatI WGTACW 5 cut(s) 89, 404, 552, 1749, 3958
TseFI GTSAC 6 cut(s) 444, 654, 1769, 2120, 3392, 4402
TseI GCWGC 8 cut(s) 1095, 1594, 2036, 2406, 3329, 3467, 3484, 4537
Tsp45I GTSAC 6 cut(s) 444, 654, 1769, 2120, 3392, 4402
TspGWI ACGGA 6 cut(s) 1257, 1441, 2100, 2161, 3226, 3690
Van91I CCANNNNNTGG 3 cut(s) 2489, 2582, 3478
Vha464I CTTAAG 2 cut(s) 1349, 4532
VpaK11BI GGWCC 6 cut(s) 559, 794, 1633, 3548, 3859, 4498
VspI ATTAAT 1 cut(s) 3269
XagI CCTNNNNNAGG 1 cut(s) 789
XapI RAATTY 8 cut(s) 1968, 2179, 3560, 3666, 4093, 4144, 4152, 4301
XceI RCATGY 9 cut(s) 678, 1336, 1702, 1739, 1849, 2509, 2977, 3491, 3610
XmiI GTMKAC 5 cut(s) 634, 2844, 2945, 2954, 4246
XmnI GAANNNNTTC 1 cut(s) 1816
ZrmI AGTACT 1 cut(s) 1751
Zsp2I ATGCAT 3 cut(s) 1905, 3139, 3724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.