MD12G1124500.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011401
Physical Location & Seq
Reverse (-)
19953224 .. 19960958
7735 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1124500.v1.1.491

Sequence Viewer

Length: 4623 bp
ATGGAGAATCGAGGTCAAAAGCGCACTGAGCTTGATGATGAATTGCCGGCAGATAAGCGGGCGTGCAATTCATTGGAGTTTAGAGCGAGTTCATCAAACTCTTCAGCTCAAACCCATATGAATTCTGTGAACTCTGCACCTGAAACCAATGATCATGATATGGACACTTCATCCTCTGCTTCGGCTAATAGTCGCTCAGAGGGAGAACATGAGAGGGATTCAGCATATGGGTCATGTGATTCAGATGATGCAGACGAAAGACACAGTGAACTCAAGGACTATCAGAGACGGAGATCTTCGGGTGATCATGGAAAGTTTAAGAGGATCTTATCGTCTTTAAGTGAAGACACTGACCCTTCTGGGCAGTTAGCTGTGCTCACCGAGCTATGTGAAGTCCTGTCATTCTGTACAGAGGACTCACTATCTGGGATGACATCAGACGCATTATCACCGCTTCTTGTTAGACTTGCTAAGCATGAGACTAACCTGGATATCATGCTATTGGCTATAAGGGCTATAACTTATTTATGCGATGTGTATCCAAAATCATCTGGCTTCCTTGTTAGGCATGATGCAGTTCCTGCTCTTTGCCAAAGACTAATGGCTATTGAATACTTGGATGTAGCTGAGCAGTGTCTACAAGCATTAGAGAAAATGTCACGGGAGCAGCCACTTGCGTGCTTACAGTCTGGGGCAATAATGGCTGTTCTGAATTATATCGATTTTTTTTCGACAAGTATACAGAGAGTTGCCCTTTCTACTGTGGTGAACATTTGTAAGAAACTTCCTTCTGAATGCCCTTCACCCTTCATGGAGGCGGTCCCCATACTGTGCAATCTTCTCCAGTATGAGGATCCACAGCTTGTCGAAAATGTTGCTGTTTGCTTGATTAAAATTACAGAACGAGTGAGCCAGTCCACTGAGATGCTGGATGAACTTTGTAAGCATGGGATTATTCGTCAAGTCACACATTTTATGAGCTTGAATACCCGACCAACTTTATCCCAGCCGCTTAGTAATGGTTTGATGGGGGTACTTGCGAAACTTTCTTCTGGTTCGGTTGTAGCCTTCAGGACTCTGTATGAGCTTAATATAAGCAGCATATTGAAGGATATCTTATCTACATATGACCTCTCTCATGGAATGTCCTCTAATCATGTGGTGGATGGGCATTGCAATCAGGTATATGAAGTTTTGAAATTGCTAAATGAGCTTCTTCCAACTTCGACCAGAGATCAAGAGAATTCACAGTTATCAGATAAGGAATCTCTTTTAGTTAATCAACCTGATCTTCTGCAAAAGTTTGGAATGGATATACTTCCATTGTTGACTCAGTTCGTAAACTCCGGTGCCAATTTATATATTTGCTATGGCTGCTTATCTGTTATTGACAAGTTAATTTATTTGAGCACATCTGACATGCTTGTAGAATTGCTTCAGAAGGCCAACATTTCAAGTTTTTTGGCTGGAGTGTTTACACGAAAGGATCCCCATGTGTTGATATTAGCTCTACATATTGCTGAGCTAATCCTGCAGAAACTTTCTGATTATTTCTTGGACTCTTTCATAAAAGAAGGCGTCTTTTTTGCCATCGAGGCACTGTTAACACCTGATAAGTGTCAATTGGTAACCCTGGAAAAATGTTCGAGGTTGTTGTTCCCTGTGTTTAGTGGCTCACAGATTTTGTTGGATCCTCGTCAGAAGTCTACTTCAAGGGAGGTCCTGAGGTGCTTGTGTTATGCTTTTGCTACTGATAGATCACCATCAGTATCAGAAAAAGGAAGTTGCATGCTTGAAAAGGATTCCGTTTATAATCTTGCAAAGCATATAAGGACCAAGTACTTTGCACAAGAATTGTATGACCCTGAGAAAGCATTAACTGATGTCCTTCAAAAGCTTAGAAAATTTTCTTCTGCAATAAGTGATTTGAACACGTCCATGAATAATGATGCTCTTGATCAGCATGAAGAAGGGTTCTATAGTATCATGCGTCAAGTTATGGAAAAGCTTGGTGGAGTAGAACCCATTTCCACTTTTGAATTTATCGAATCTGGAATTCTAAGGTCCTTATTGACTTATCTGTCCAATGGCCAGTACTTGAAACAAAAGGGGGAACTTAGTGCTGGTAACAGTGATATTTATTCTGTAGAAAAAAGATTTGAGGTGTTTGCAAGGCTATTATTTTCTCCTTTAGACATGATCACTGCGGACCTGCCCATGATAACATTAATACGCAAACTGCAAAATGCGCTATCTTCTTTGGAAAATTTCCCTGTTATTTTGAGCAATGTGCCCAAGCTAAGAAGTTCATATGCTACACTTCCATATGGGCGTCGCACAACATATCCATGTTTTAAAGTTCGTTTTGTGAAAGACAAGGGGGAGACGCATCTTTGTGATTACCGTGAAGGTGTTCTGACTGTGGACCCTTTTTCCTCATTGCATGCCATTGAAGAAGTTTTGTGGCCTAAAGTCAAAGCAAAAAGAACCAGCCACATTAAATCACCTACACAAGTTAAGGACCAATCTGAAAGTCTACCGGATCAATCACCATCAAATGCAAGCTCTAGTCAAGGGGGAAGTCCACATCCTATGGAGCCTGAAAGCATGTCGACAGACTTGCCTGAGTTGCAGGAACCTGTGGAAAAAGAAGCACAATGTGCTTCAGAGGAAGACACTGAGATGGAAGAACAGTATCCAGTATCTTGTAGCAAAGAAGATTCTTCTTCAAAACTGTTATTTTATCTTGAAGGGCAACAGCTGGAGCCGTCATTGACATTGTACCAAGCAATTCTGCAACAACAAATGAACGAACATGAAATTGTCATTGGTTCAAAACTTTGGAGTCAAGAATACACATTAACATACAGTAAGGCTGAAGGTCAATACGGTACACGTAAAGAATGTCTTTGTTCAGCAGAAAGTTCTGCTGAGAAAGTTGACGTACATGAGTTGTATACTTCATTTTTCTCCAGCATGTTTGCTTATGATCTTGCTTCTGACTTGGAGAAGTCAAGTCCTGTTTATGATATAATATACATCCTCAAAAGCTTGGAAAGGATGAATAAAGTTATATTTCATCTGATGTCTCGTGAAAGAATATGTGCTTTTGCTAAAGGGAAAATCAATGACTTGGATAATTTTCAGACAGCAGTTATTCCAGTGCCACAGAATGAGTTCGTGAGCAGTAAATTGACAGAAAAATTGGAACAACAGATGCGGGATGCTTTAGCAGTGTCCATTGGTGGTATGCCTTTGTGGTGTAATCAGTTAATGGAATCGTGTCCCTTTTTATTTAGTTTTGAGGTCAAATGTAAGTACTTCCGTTTGGCAGCATTTGGTCCACTGCTAGGTCAGCCTCATTCACCGTCTTATAGGGATTCAGGGGTGACCAGTGACAGGAGACTAAGCTCCGGAAGCATGCCTCGAAAAAAGTTTTTGGTTTTCCGCAACCAAATTCTGGATTCTGCTGCCCAAATGATGGACCTGCATGCCAGGCAGAAGGTGCTCCTTGAGGTGGAATACAATGAAGAAGTTGGTACTGGTCTTGGTCCAACCTTGGAATTCTACACCTTGGTTAGTCACGAGTTTCAGAAGTCTGGTCTAGGCATGTGGAGAGATGATCGTGGGTCTTTTACCACTGGTACAAGCCATGCTGGGGACAGTGGAATTTTGATATGTCCTTTTGGTCTTTTCCCCTGTCCATGGTTGGGTACATCTGATGAGATGCAGATACAGTTCAGTGAAGTAATTAAGAAGTTTGTCCTTTTGGGGCAAATCGTAGGAAAAGCGCTCCAAGATGGGCGTGTCTTGGATGTGCATTTCTCCAAAGCCTTCTATAAACTTCTTCTTGGCAAGGAACTTGGTGTGTATGACATCCTGTCATTTGATCCTGAGCTTGGCAAGACACTGCTAGAGTTTAAGGCTCTCGTAGACAGGAAAAGATTTTCGGAGTCCATTCATGGAGAAAGTACAACATTGAAATTTGACTCATGCTTTCGGAAAACCCAAATTGAGGATCTTTGTCTTGATTTTACACTTCCTGGCTATCCTGATTTTATTCTTTCTTCTAGGCCTGATCATAAAATGGTAAATATTACAAACTTGGAAGATTATGTTTCACTTGTGGCGGATGCAACTGTAACTGCTGGAATTTCTAGACAAGTGGAAGCTTTCAAATCTGGCTTTAACCAGGTTTTCCCTATTGAGCATCTTCAGATTTTCACCGAAGAAGAATTAGAGCGTTTATTATGTGGAGAACGTGATTCTTGGGCTTTCAATGAGCTCCTTGATCATATCAAATTCGACCATGGTTACACAGTTAGCAGTCCTCCCATTATCAATTTGCTGGAAATCATACACAAGTTCGACCAAGAACAGCGAAGAGCGTTTTTGCAATTTGTGACTGGGGCACCTCGCTTACCTCCTGGAGGTTTTGCATCTCTCAGTCCGAAGTTAACTATTGTTCGAAAGCAATCTAGCAACTGCGCAGACTTAGACCTACCAAGTGTGATGACCTGTGCAAATTATCTTAAGCTGCCGCCTTACTCTTCACAAGAGACGATGAAAGAGAAGCTCTTATATGCCATTACAGAAGGGCAGGGATCGTTCCACCTTTCGTAG

Protein Analysis

1541

Amino Acids

172.9

Weight (kDa)

5.26

Isoelectric Point (pI)

52.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_TRIP12_N PF25579 4 - 229 4.2e-118 E3 ubiquitin-protein ligase TRIP12-like, TPR repeats
HECT PF00632 1228 - 1540 8.5e-80 HECT-domain (ubiquitin-transferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011401)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02880 AT5G02880
fragaria_vesca FvH4_6g15270 FvH4_6g15270 FvH4_6g15270 FvH4_6g15270
malus_domestica MD04G1104600.v1.1 MD12G1124500.v1.1
prunus_persica Prupe.6G241700_v2.0.a1 Prupe.6G241700_v2.0.a1
pyrus_communis pycom12g12310
rosa_chinensis RchiOBHm_Chr3g0468671
rosa_laevigata RLG00000024416
rosa_multiflora Rmu_sc0011538.1_g000007
rosa_roxburghii Rroxscaffold_6G00412660
rosa_rugosa Rorug03G0096200
rosa_samantha Rh3AG145000 Rh3BG167500 Rh3DG166300
rosa_wichuraiana Rw3G013670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1812
AasI GACNNNNNNGTC 1 cut(s) 2080
Acc16I TGCGCA 1 cut(s) 4489
Acc36I ACCTGC 2 cut(s) 2220, 3495
AccB1I GGYRCC 2 cut(s) 1349, 4411
AccB7I CCANNNNNTGG 2 cut(s) 3460, 3481
AccI GTMKAC 7 cut(s) 637, 739, 1706, 2536, 2612, 2957, 3933
AccIII TCCGGA 1 cut(s) 3413
AciI CCGC 9 cut(s) 58, 452, 818, 1010, 2207, 3220, 3448, 4130, 4541
AcoI YGGCCR 1 cut(s) 2089
AcuI CTGAAG 6 cut(s) 87, 1054, 1421, 2649, 2898, 4199
AcyI GRCGYC 2 cut(s) 1578, 2332
AdeI CACNNNGTG 1 cut(s) 2660
AfeI AGCGCT 1 cut(s) 3792
AflII CTTAAG 1 cut(s) 4532
AflIII ACRYGT 2 cut(s) 1932, 2894
AhdI GACNNNNNGTC 1 cut(s) 3880
AjiI CACGTC 1 cut(s) 1935
AjnI CCWGG 6 cut(s) 486, 1632, 3494, 4042, 4191, 4426
AjuI GAANNNNNNNTTGG 2 cut(s) 2811, 2843
AleI CACNNNNGTG 1 cut(s) 676
Alw21I GWGCWC 4 cut(s) 378, 1412, 3510, 4287
Alw26I GTCTC 6 cut(s) 280, 473, 2378, 3093, 3397, 4553
AlwNI CAGNNNCTG 1 cut(s) 581
Aor13HI TCCGGA 1 cut(s) 3413
Aor51HI AGCGCT 1 cut(s) 3792
AoxI GGCC 4 cut(s) 1443, 2089, 2465, 4073
ApeKI GCWGC 6 cut(s) 667, 1098, 1374, 3332, 3470, 4537
AseI ATTAAT 1 cut(s) 2228
Asp700I GAANNNNTTC 4 cut(s) 1434, 1906, 2412, 3176
AspLEI GCGC 4 cut(s) 24, 2251, 3793, 4490
AsuII TTCGAA 1 cut(s) 4468
AxyI CCTNAGG 1 cut(s) 1724
BaeGI GKGCMC 2 cut(s) 2295, 4414
BalI TGGCCA 1 cut(s) 2091
BamHI GGATCC 3 cut(s) 853, 1486, 1690
BanI GGYRCC 2 cut(s) 1349, 4411
BanII GRGCYC 1 cut(s) 4287
BarI GAAGNNNNNNTAC 2 cut(s) 1419, 1451
BauI CACGAG 2 cut(s) 3090, 3584
BbsI GAAGAC 2 cut(s) 351, 2679
Bbv12I GWGCWC 4 cut(s) 378, 1412, 3510, 4287
BbvI GCAGC 6 cut(s) 679, 1110, 1361, 3344, 3457, 4524
BccI CCATC 8 cut(s) 1021, 1160, 1598, 1771, 2560, 2677, 3475, 3794
BceAI ACGGC 1 cut(s) 2752
BcgI CGANNNNNNTGC 4 cut(s) 857, 891, 2602, 2636
BciT130I CCWGG 6 cut(s) 488, 1634, 3496, 4044, 4193, 4428
BciVI GTATCC 2 cut(s) 549, 2706
BclI TGATCA 6 cut(s) 151, 304, 1957, 2199, 4078, 4291
BcoDI GTCTC 6 cut(s) 280, 473, 2378, 3093, 3397, 4553
BfaI CTAG 7 cut(s) 2568, 3350, 3605, 3914, 4071, 4158, 4479
BfmI CTRYAG 3 cut(s) 1532, 1978, 2145
BfoI RGCGCY 1 cut(s) 3794
BfrI CTTAAG 1 cut(s) 4532
BfuAI ACCTGC 2 cut(s) 2220, 3495
BfuI GTATCC 2 cut(s) 549, 2706
BglI GCCNNNNNGGC 1 cut(s) 1595
BglII AGATCT 1 cut(s) 293
BisI GCNGC 8 cut(s) 668, 1010, 1099, 1375, 3333, 3471, 4538, 4541
BlpI GCTNAGC 3 cut(s) 471, 627, 1521
BlsI GCNGC 8 cut(s) 669, 1011, 1100, 1376, 3334, 3472, 4539, 4542
BmcAI AGTACT 3 cut(s) 1841, 2096, 3320
Bme1390I CCNGG 6 cut(s) 488, 1634, 3496, 4044, 4193, 4428
BmeRI GACNNNNNGTC 1 cut(s) 3880
BmgBI CACGTC 1 cut(s) 1935
BmrFI CCNGG 6 cut(s) 488, 1634, 3496, 4044, 4193, 4428
BmrI ACTGGG 1 cut(s) 4416
BmuI ACTGGG 1 cut(s) 4416
BpiI GAAGAC 2 cut(s) 351, 2679
BpmI CTGGAG 5 cut(s) 827, 1488, 2783, 2956, 4449
Bpu10I CCTNAGC 1 cut(s) 3894
Bpu1102I GCTNAGC 3 cut(s) 471, 627, 1521
Bpu14I TTCGAA 1 cut(s) 4468
BpuEI CTTGAG 2 cut(s) 257, 3533
Bsa29I ATCGAT 1 cut(s) 720
BsaAI YACGTR 1 cut(s) 2897
BsaBI GATNNNNATC 2 cut(s) 537, 1762
BsaHI GRCGYC 2 cut(s) 1578, 2332
BsaJI CCNNGG 5 cut(s) 1632, 3558, 3573, 3704, 4309
BsaWI WCCGGW 3 cut(s) 1346, 2539, 3413
Bse118I RCCGGY 1 cut(s) 46
Bse1I ACTGG 9 cut(s) 844, 913, 2092, 2699, 3161, 3393, 3547, 3646, 4411
Bse21I CCTNAGG 1 cut(s) 1724
Bse3DI GCAATG 3 cut(s) 1171, 2293, 2438
Bse8I GATNNNNATC 2 cut(s) 537, 1762
BseAI TCCGGA 1 cut(s) 3413
BseBI CCWGG 6 cut(s) 488, 1634, 3496, 4044, 4193, 4428
BseCI ATCGAT 1 cut(s) 720
BseDI CCNNGG 5 cut(s) 1632, 3558, 3573, 3704, 4309
BseJI GATNNNNATC 2 cut(s) 537, 1762
BseMI GCAATG 3 cut(s) 1171, 2293, 2438
BseNI ACTGG 9 cut(s) 844, 913, 2092, 2699, 3161, 3393, 3547, 3646, 4411
BseSI GKGCMC 2 cut(s) 2295, 4414
BseXI GCAGC 6 cut(s) 679, 1110, 1361, 3344, 3457, 4524
BseYI CCCAGC 2 cut(s) 1005, 3656
BsgI GTGCAG 1 cut(s) 120
BshFI GGCC 4 cut(s) 1445, 2091, 2467, 4075
BshNI GGYRCC 2 cut(s) 1349, 4411
BshVI ATCGAT 1 cut(s) 720
BsiHKAI GWGCWC 4 cut(s) 378, 1412, 3510, 4287
BsiSI CCGG 4 cut(s) 47, 1347, 2540, 3414
BslFI GGGAC 3 cut(s) 806, 3270, 3674
BsmAI GTCTC 6 cut(s) 280, 473, 2378, 3093, 3397, 4553
BsmBI CGTCTC 3 cut(s) 280, 2378, 4553
BsmFI GGGAC 3 cut(s) 806, 3270, 3674
BsmI GAATGC 1 cut(s) 800
BsnI GGCC 4 cut(s) 1445, 2091, 2467, 4075
Bsp119I TTCGAA 1 cut(s) 4468
Bsp1286I GDGCHC 6 cut(s) 378, 1412, 2295, 3510, 4287, 4414
Bsp13I TCCGGA 1 cut(s) 3413
Bsp1407I TGTACA 1 cut(s) 407
Bsp1720I GCTNAGC 3 cut(s) 471, 627, 1521
Bsp19I CCATGG 2 cut(s) 3704, 4309
BspACI CCGC 9 cut(s) 58, 452, 818, 1010, 2207, 3220, 3448, 4130, 4541
BspANI GGCC 4 cut(s) 1445, 2091, 2467, 4075
BspDI ATCGAT 1 cut(s) 720
BspEI TCCGGA 1 cut(s) 3413
BspHI TCATGA 1 cut(s) 154
BspMAI CTGCAG 1 cut(s) 1536
BspMI ACCTGC 2 cut(s) 2220, 3495
BspQI GCTCTTC 1 cut(s) 4378
BspT104I TTCGAA 1 cut(s) 4468
BspT107I GGYRCC 2 cut(s) 1349, 4411
BspTI CTTAAG 1 cut(s) 4532
BsrDI GCAATG 3 cut(s) 1171, 2293, 2438
BsrFI RCCGGY 1 cut(s) 46
BsrGI TGTACA 1 cut(s) 407
BsrI ACTGG 9 cut(s) 844, 913, 2092, 2699, 3161, 3393, 3547, 3646, 4411
BssAI RCCGGY 1 cut(s) 46
BssECI CCNNGG 5 cut(s) 1632, 3558, 3573, 3704, 4309
BssNAI GTATAC 2 cut(s) 740, 2958
BssNI GRCGYC 2 cut(s) 1578, 2332
BssSI CACGAG 2 cut(s) 3090, 3584
BssT1I CCWWGG 4 cut(s) 3558, 3573, 3704, 4309
Bst1107I GTATAC 2 cut(s) 740, 2958
Bst2BI CACGAG 2 cut(s) 3090, 3584
Bst2UI CCWGG 6 cut(s) 488, 1634, 3496, 4044, 4193, 4428
Bst6I CTCTTC 3 cut(s) 106, 4378, 4555
BstACI GRCGYC 2 cut(s) 1578, 2332
BstAFI CTTAAG 1 cut(s) 4532
BstAPI GCANNNNNTGC 3 cut(s) 581, 2660, 3505
BstAUI TGTACA 1 cut(s) 407
BstBAI YACGTR 1 cut(s) 2897
BstBI TTCGAA 1 cut(s) 4468
BstC8I GCNNGC 9 cut(s) 48, 60, 64, 679, 1790, 2445, 2563, 3422, 3492
BstDSI CCRYGG 2 cut(s) 3704, 4309
BstEII GGTNACC 2 cut(s) 1627, 3388
BstENI CCTNNNNNAGG 1 cut(s) 4428
BstH2I RGCGCY 1 cut(s) 3794
BstHHI GCGC 4 cut(s) 24, 2251, 3793, 4490
BstMAI GTCTC 6 cut(s) 280, 473, 2378, 3093, 3397, 4553
BstNI CCWGG 6 cut(s) 488, 1634, 3496, 4044, 4193, 4428
BstNSI RCATGY 8 cut(s) 1423, 1792, 2447, 2611, 2980, 3424, 3494, 3613
BstPI GGTNACC 2 cut(s) 1627, 3388
BstSCI CCNGG 6 cut(s) 486, 1632, 3494, 4042, 4191, 4426
BstSFI CTRYAG 3 cut(s) 1532, 1978, 2145
BstSLI GKGCMC 2 cut(s) 2295, 4414
BstV1I GCAGC 6 cut(s) 679, 1110, 1361, 3344, 3457, 4524
BstV2I GAAGAC 2 cut(s) 351, 2679
BstX2I RGATCY 6 cut(s) 293, 324, 853, 1486, 1690, 4018
BstYI RGATCY 6 cut(s) 293, 324, 853, 1486, 1690, 4018
BstZ17I GTATAC 2 cut(s) 740, 2958
Bsu15I ATCGAT 1 cut(s) 720
Bsu36I CCTNAGG 1 cut(s) 1724
BsuI GTATCC 2 cut(s) 549, 2706
BsuRI GGCC 4 cut(s) 1445, 2091, 2467, 4075
BsuTUI ATCGAT 1 cut(s) 720
BtgI CCRYGG 2 cut(s) 3704, 4309
BtgZI GCGATG 1 cut(s) 546
BtrI CACGTC 1 cut(s) 1935
BtsI GCAGTG 5 cut(s) 638, 2202, 3240, 3344, 3908
BveI ACCTGC 2 cut(s) 2220, 3495
Cac8I GCNNGC 9 cut(s) 48, 60, 64, 679, 1790, 2445, 2563, 3422, 3492
CaiI CAGNNNCTG 1 cut(s) 581
CciI TCATGA 1 cut(s) 154
CfoI GCGC 4 cut(s) 24, 2251, 3793, 4490
Cfr10I RCCGGY 1 cut(s) 46
ClaI ATCGAT 1 cut(s) 720
CseI GACGC 5 cut(s) 449, 1567, 1979, 2321, 2395
CsiI ACCWGGT 1 cut(s) 4191
DraI TTTAAA 1 cut(s) 2356
DraIII CACNNNGTG 1 cut(s) 2660
DrdI GACNNNNNNGTC 1 cut(s) 2080
DriI GACNNNNNGTC 1 cut(s) 3880
DseDI GACNNNNNNGTC 1 cut(s) 2080
EaeI YGGCCR 1 cut(s) 2089
Eam1104I CTCTTC 3 cut(s) 106, 4378, 4555
Eam1105I GACNNNNNGTC 1 cut(s) 3880
EarI CTCTTC 3 cut(s) 106, 4378, 4555
EciI GGCGGA 1 cut(s) 4145
Ecl136II GAGCTC 1 cut(s) 4285
Eco130I CCWWGG 4 cut(s) 3558, 3573, 3704, 4309
Eco147I AGGCCT 1 cut(s) 4075
Eco24I GRGCYC 1 cut(s) 4287
Eco32I GATATC 2 cut(s) 493, 1114
Eco47III AGCGCT 1 cut(s) 3792
Eco53kI GAGCTC 1 cut(s) 4285
Eco57I CTGAAG 6 cut(s) 87, 1054, 1421, 2649, 2898, 4199
Eco81I CCTNAGG 1 cut(s) 1724
Eco91I GGTNACC 2 cut(s) 1627, 3388
EcoICRI GAGCTC 1 cut(s) 4285
EcoNI CCTNNNNNAGG 1 cut(s) 4428
EcoO109I RGGNCCY 2 cut(s) 1720, 2064
EcoO65I GGTNACC 2 cut(s) 1627, 3388
EcoRI GAATTC 4 cut(s) 121, 1243, 2055, 3563
EcoRII CCWGG 6 cut(s) 486, 1632, 3494, 4042, 4191, 4426
EcoRV GATATC 2 cut(s) 493, 1114
EcoT14I CCWWGG 4 cut(s) 3558, 3573, 3704, 4309
EcoT38I GRGCYC 1 cut(s) 4287
ErhI CCWWGG 4 cut(s) 3558, 3573, 3704, 4309
Esp3I CGTCTC 3 cut(s) 280, 2378, 4553
FalI AAGNNNNNCTT 6 cut(s) 311, 343, 1100, 1132, 2892, 2924
FaqI GGGAC 3 cut(s) 806, 3270, 3674
FauI CCCGC 2 cut(s) 51, 3213
FauNDI CATATG 5 cut(s) 117, 226, 1126, 2311, 2326
FbaI TGATCA 6 cut(s) 151, 304, 1957, 2199, 4078, 4291
FblI GTMKAC 7 cut(s) 637, 739, 1706, 2536, 2612, 2957, 3933
Fnu4HI GCNGC 8 cut(s) 668, 1010, 1099, 1375, 3333, 3471, 4538, 4541
FriOI GRGCYC 1 cut(s) 4287
Fsp4HI GCNGC 8 cut(s) 668, 1010, 1099, 1375, 3333, 3471, 4538, 4541
FspBI CTAG 7 cut(s) 2568, 3350, 3605, 3914, 4071, 4158, 4479
FspI TGCGCA 1 cut(s) 4489
GlaI GCGC 4 cut(s) 23, 2250, 3792, 4489
GluI GCNGC 8 cut(s) 668, 1010, 1099, 1375, 3333, 3471, 4538, 4541
GsaI CCCAGC 2 cut(s) 1009, 3660
GsuI CTGGAG 5 cut(s) 827, 1488, 2783, 2956, 4449
HaeII RGCGCY 1 cut(s) 3794
HaeIII GGCC 4 cut(s) 1445, 2091, 2467, 4075
HapII CCGG 4 cut(s) 47, 1347, 2540, 3414
HgaI GACGC 5 cut(s) 449, 1567, 1979, 2321, 2395
HhaI GCGC 4 cut(s) 24, 2251, 3793, 4490
Hin1I GRCGYC 2 cut(s) 1578, 2332
Hin6I GCGC 4 cut(s) 22, 2249, 3791, 4488
HinP1I GCGC 4 cut(s) 22, 2249, 3791, 4488
HincII GTYRAC 5 cut(s) 1329, 1605, 2613, 2941, 4458
HindII GTYRAC 5 cut(s) 1329, 1605, 2613, 2941, 4458
HindIII AAGCTT 4 cut(s) 1895, 2006, 3049, 4170
HpaI GTTAAC 2 cut(s) 1605, 4458
HpaII CCGG 4 cut(s) 47, 1347, 2540, 3414
Hpy99I CGWCG 1 cut(s) 2337
HpyCH4IV ACGT 4 cut(s) 1934, 2896, 2943, 4261
HpySE526I ACGT 4 cut(s) 1934, 2896, 2943, 4261
Hsp92I GRCGYC 2 cut(s) 1578, 2332
HspAI GCGC 4 cut(s) 22, 2249, 3791, 4488
Kpn2I TCCGGA 1 cut(s) 3413
KroI GCCGGC 1 cut(s) 46
KroNI GCCGGC 1 cut(s) 48
Ksp22I TGATCA 6 cut(s) 151, 304, 1957, 2199, 4078, 4291
KspAI GTTAAC 2 cut(s) 1605, 4458
LguI GCTCTTC 1 cut(s) 4378
LmnI GCTCC 7 cut(s) 664, 2596, 2764, 3416, 3513, 3798, 4290
Lsp1109I GCAGC 6 cut(s) 679, 1110, 1361, 3344, 3457, 4524
MabI ACCWGGT 1 cut(s) 4191
MaeI CTAG 7 cut(s) 2568, 3350, 3605, 3914, 4071, 4158, 4479
MaeII ACGT 4 cut(s) 1934, 2896, 2943, 4261
MfeI CAATTG 1 cut(s) 1622
MflI RGATCY 6 cut(s) 293, 324, 853, 1486, 1690, 4018
MhlI GDGCHC 6 cut(s) 378, 1412, 2295, 3510, 4287, 4414
MlsI TGGCCA 1 cut(s) 2091
MluNI TGGCCA 1 cut(s) 2091
MlyI GAGTC 7 cut(s) 410, 1069, 1324, 1553, 2854, 3962, 3983
MmeI TCCRAC 3 cut(s) 1244, 1668, 3578
Mox20I TGGCCA 1 cut(s) 2091
MroI TCCGGA 1 cut(s) 3413
MroNI GCCGGC 1 cut(s) 46
MroXI GAANNNNTTC 4 cut(s) 1434, 1906, 2412, 3176
MscI TGGCCA 1 cut(s) 2091
MslI CAYNNNNRTG 6 cut(s) 676, 923, 1937, 2347, 2394, 2681
Msp20I TGGCCA 1 cut(s) 2091
MspA1I CMGCKG 1 cut(s) 2761
MspCI CTTAAG 1 cut(s) 4532
MspI CCGG 4 cut(s) 47, 1347, 2540, 3414
MspR9I CCNGG 6 cut(s) 488, 1634, 3496, 4044, 4193, 4428
MunI CAATTG 1 cut(s) 1622
Mva1269I GAATGC 1 cut(s) 800
MvaI CCWGG 6 cut(s) 488, 1634, 3496, 4044, 4193, 4428
NaeI GCCGGC 1 cut(s) 48
NcoI CCATGG 2 cut(s) 3704, 4309
NdeI CATATG 5 cut(s) 117, 226, 1126, 2311, 2326
NgoMIV GCCGGC 1 cut(s) 46
NmuCI GTSAC 6 cut(s) 657, 964, 3388, 3395, 3581, 4402
NsbI TGCGCA 1 cut(s) 4489
NspI RCATGY 8 cut(s) 1423, 1792, 2447, 2611, 2980, 3424, 3494, 3613
NspV TTCGAA 1 cut(s) 4468
OliI CACNNNNGTG 1 cut(s) 676
PaeI GCATGC 4 cut(s) 1792, 2447, 3424, 3494
PagI TCATGA 1 cut(s) 154
PceI AGGCCT 1 cut(s) 4075
PciSI GCTCTTC 1 cut(s) 4378
PctI GAATGC 1 cut(s) 800
PdiI GCCGGC 1 cut(s) 48
PdmI GAANNNNTTC 4 cut(s) 1434, 1906, 2412, 3176
PflMI CCANNNNNTGG 2 cut(s) 3460, 3481
PfoI TCCNGGA 1 cut(s) 4426
PkrI GCNGC 8 cut(s) 669, 1011, 1100, 1376, 3334, 3472, 4539, 4542
PleI GAGTC 7 cut(s) 410, 1069, 1324, 1553, 2853, 3961, 3983
PpsI GAGTC 7 cut(s) 410, 1069, 1324, 1553, 2853, 3961, 3983
Ppu21I YACGTR 1 cut(s) 2897
PpuMI RGGWCCY 2 cut(s) 1720, 2064
PshBI ATTAAT 1 cut(s) 2228
PsiI TTATAA 1 cut(s) 1812
Psp124BI GAGCTC 1 cut(s) 4287
Psp5II RGGWCCY 2 cut(s) 1720, 2064
Psp6I CCWGG 6 cut(s) 486, 1632, 3494, 4042, 4191, 4426
PspEI GGTNACC 2 cut(s) 1627, 3388
PspFI CCCAGC 2 cut(s) 1005, 3656
PspGI CCWGG 6 cut(s) 486, 1632, 3494, 4042, 4191, 4426
PspPPI RGGWCCY 2 cut(s) 1720, 2064
PstI CTGCAG 1 cut(s) 1536
PstNI CAGNNNCTG 1 cut(s) 581
PsuI RGATCY 6 cut(s) 293, 324, 853, 1486, 1690, 4018
PvuII CAGCTG 1 cut(s) 2761
RseI CAYNNNNRTG 6 cut(s) 676, 923, 1937, 2347, 2394, 2681
SacI GAGCTC 1 cut(s) 4287
SalI GTCGAC 1 cut(s) 2611
SapI GCTCTTC 1 cut(s) 4378
SatI GCNGC 8 cut(s) 668, 1010, 1099, 1375, 3333, 3471, 4538, 4541
ScaI AGTACT 3 cut(s) 1841, 2096, 3320
SchI GAGTC 7 cut(s) 410, 1069, 1324, 1553, 2854, 3962, 3983
ScrFI CCNGG 6 cut(s) 488, 1634, 3496, 4044, 4193, 4428
SduI GDGCHC 6 cut(s) 378, 1412, 2295, 3510, 4287, 4414
SexAI ACCWGGT 1 cut(s) 4191
SfcI CTRYAG 3 cut(s) 1532, 1978, 2145
SfuI TTCGAA 1 cut(s) 4468
SmiMI CAYNNNNRTG 6 cut(s) 676, 923, 1937, 2347, 2394, 2681
SmlI CTYRAG 3 cut(s) 272, 3512, 4532
SmoI CTYRAG 3 cut(s) 272, 3512, 4532
SphI GCATGC 4 cut(s) 1792, 2447, 3424, 3494
SseBI AGGCCT 1 cut(s) 4075
SsiI CCGC 9 cut(s) 58, 452, 818, 1010, 2207, 3220, 3448, 4130, 4541
SspI AATATT 1 cut(s) 4096
SspMI CTAG 7 cut(s) 2568, 3350, 3605, 3914, 4071, 4158, 4479
SstI GAGCTC 1 cut(s) 4287
StuI AGGCCT 1 cut(s) 4075
StyD4I CCNGG 6 cut(s) 486, 1632, 3494, 4042, 4191, 4426
StyI CCWWGG 4 cut(s) 3558, 3573, 3704, 4309
TaiI ACGT 4 cut(s) 1937, 2899, 2946, 4264
TatI WGTACW 5 cut(s) 407, 1839, 2094, 3318, 3971
TauI GCSGC 2 cut(s) 1012, 4543
TseFI GTSAC 6 cut(s) 657, 964, 3388, 3395, 3581, 4402
TseI GCWGC 6 cut(s) 667, 1098, 1374, 3332, 3470, 4537
Tsp45I GTSAC 6 cut(s) 657, 964, 3388, 3395, 3581, 4402
TspGWI ACGGA 3 cut(s) 304, 1795, 3314
Van91I CCANNNNNTGG 2 cut(s) 3460, 3481
Vha464I CTTAAG 1 cut(s) 4532
VspI ATTAAT 1 cut(s) 2228
XagI CCTNNNNNAGG 1 cut(s) 4428
XbaI TCTAGA 1 cut(s) 4157
XceI RCATGY 8 cut(s) 1423, 1792, 2447, 2611, 2980, 3424, 3494, 3613
XcmI CCANNNNNNNNNTGG 1 cut(s) 925
XmiI GTMKAC 7 cut(s) 637, 739, 1706, 2536, 2612, 2957, 3933
XmnI GAANNNNTTC 4 cut(s) 1434, 1906, 2412, 3176
XspI CTAG 7 cut(s) 2568, 3350, 3605, 3914, 4071, 4158, 4479
ZrmI AGTACT 3 cut(s) 1841, 2096, 3320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.