Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0011401

Physical Location & Seq

Genomic Coordinates Forward (+)

14482081 .. 14489461

Gene Structure 7381 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG166300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4416 bp

ATGGAAAGTCGTGGCCAAAAGCGGTCTGAGATTGATGATGAATTGCCGGCCGATAAGAGGGCCTGTAGTTCTTTGGAGTTTAGACCGAGTACATCCAGCTCGTCTCAAACCCCTATCAATTCTGTGAGCTCCACGCCCGAACGCAATGATCAGAACAATCATGATCATGCTGATCATATGGATACTGAATCCGAAGGAGGAGAGCCTGAGAAGGATTCAGCGTATGATTCTTGTGATGAGGATGAGGAAGATCAGAGACACAGTGATCTTAGGGACTATCAGAGGCGGAGAGCATCCGGTGATCATGGAAAGTTTCAGACCATAATATCATCTCTGAGTGAAGAAGTCGATCTTTCTCAGCACCTGGTTATGCTCACCGAGTTATGCGAAGTGTTATCTTTTTGTACAGAGGATTCACTGTCAGGCGATACATCGAACTCGTTGTCACCGATTCTTGTTAAGCTGTCAAGGGATGAGACTAGCGCGGATATCATGCTATTGGCTATAAGGGCAATGACTTATTTATGTGATGTATATCCGAAATCATCGGTGTACTTGGTCCGACATGATGCAGTGTCTGCTCTTTGCCAAAGACTTTTGGCTATTGAGTATTTGGATGTAGCTGAGCAATGTCTACAAGCATTGGAGAAGTTGTCACGGGAGCAACCACTTGCATGCTTACAGGCTGGAGCAATAATGGCTGTTCTGAATTATATAGACTTCTTCTCTACAAGCATACAGAGAGTTGCCCTTTCTACTGTGGTGAACATTTGTAAGAAACTTCCTTCTGAAGGTCCTTCACCTTTCATGGACGCTGTTCCTACATTGTGCAACCTTCTTCAATATGAGGATCCACAGCTTGTCGAAAATGTTGCTATTTGCTTGATTAAAATTACAGAGCGAGTGAGTCAGTCTTCTGAGATGCTGGATGAACTTTGTAAGCATGGGCTGATTCGTCAAGCCACACATTTTATGAGCTTAAATAGCCGAACTACTCTGTCCCAACCAATTCATAATGGTTTGATAGGGCTACTTGTCAAACTTTCTTCCGGTTCAGTTGTAGCCTTCAGGACTCTATATGAGCTTAATATAAGCAGCATATTAAAGGATATATTATCCACTTATGACCTCTCACATGGAATGTCCTCTACTCATGTTGTGGATGGGCATTGCTATCAGGTATATGAAGTTCTGAAGTTACTAAATGAGCTTCTCCCAACTTCAGCCAGAGATCAAGATGCTCCACAGTTGTCAGAGAAAGAATCTTTTTTAATAAATCGGCCTGAACTTCTGGAAGACTTTGGGAGGGATATACTTCCGTCTCTGATACAGGTTGTAAATTCCGGTGCCAATTTATATATTTGCTATGGATGCTTGTCAGTCATCAACAAGTTAATTTATTTGAGCACATCTGACATGCTCGTTGAACTGCTTAAGAATGCCAACATTTCAAGTTTTCTGGCTGGAGTCTTTACTCGAAAGGATCCTCATGTGTTGGTATCAACCCTACGGATTGCTGAGTTAATCCTGCAAAAGTTCTCTGATAATTTCTTGGACTCATTTATTAAAGAAGGTGTCTTTTTCGCCATTGATGCTTTGCTAACCCCTGAAAAAAGCTCACTAGTAACACTGAACAAATGTTCGAAGCTGGTGTTTCCAGTGTCTAGTGGAACAAGGCTGCTGTCTGACTCTAGTCAGAAGTCTGCTTCAAAAGAGGTCCTGAGATGCTTATGTTATGCTTTTCCTAGCAGTTCCCCATCAGGGTCAGAAAAAGGAAGTTGCATGCTTGAAAAGGATTCTGTTTATAGTCTTGCAAAGCATGTTAGAACCAAGTACTTTGCACCAGAATTATGTGACCCTGATAAATCATTAACTGATGTTCTTCAGAAGCTTAGAACTTTTTCTGCTTCATTAAGTGATTTGATGAACACGTCCTTGGATACTTGTGCTCCTAATCAGCATGAAGAGAGCTTCTATGGTATAATGCATCAAGTAATGGAAAAGCTTGGTGGAAGAGAACCCATTTCCACTTTTGAATTGATTGAAAGTGGAATTCTGAAGTCCTTGATGACTTACTTGTCCAATGACCGGTATTTGAGACAGAAGCGTGATCTTGTAGCTGCTAAAGGTGATATCTATGCTGTAGAAAAAAGATTTGAGGTGTTCGCAAGGCTATTATTTTCTTCGCCAGATCCGTTCTCTGGTGACCTGCCCATTGTAACATTGATACGGAGATTACAAAGCTCATTATCTACTTTGGAAAATTTCCCCGTTATCTTAAGCCATATACCCAAGCAGAGGAACTCATATGCTACAGTTCCATATGAGCGATACACTGCATATCCATGTATGAGAGCTCGTTTTGTTAGAGACAAGGAAGAGACTACCCTTGGTGATTGCTGTGAAGATGTTTTGACCGTTGACCCTTTTTCTTCATTGGATGCCATTGAAGGATATGTGTGGCCTAAAGTCAATGCAAAAGGAACCAGCCGCATCAAATCAGCTACAATTGAGGGTCAATCAGAATGCACACCATCAAATGCCAGCTCTAGTCAAGGAGGGTGTCCAAATACTGGTGAGCCTGAGAGCATGTCCGCAGACTTGCCTGAGTTGAAGGCAGATGAGGTCAATTTAACACAGCCGGAACCTGAACAAGAACCAAGTTTTGGACAGACAAATCCTGGAACATCTTTAGATGAAACTTATACTGATACTGTGGAGAAGGAAGTAGAAGCTCAATCTGAGGAAGACACACAGATGGAAGAACAGTGTCCTTTATCTTGTAGCAATGAAGATGCTTCACTGAAATTGTTGTTTTACCTTGAAGGGAAACAGCTGGAACGGTCATTGACATTGTATCAGGCAATTCTTCAACAACAAATGAAAGAACATGAAATTGTCATTGGTTCAAAACTGTGGAGTAAAGTATACACACTGACATATAGAAAAGCTGTAGGTCAAGAAGGTGCTCATAATGAAGGTGTTGACTCGGCAGAGAGTTCCACTGTATCAGATAAAGTTGGGGTGTATACGTTGTATACTTCACTTTTCTCCAACATGTTTCCTTGTGAGCTTGCTTCTGACTTGGAGAAGTTGAATCCTACCTATGACATTGTATATCTGCTCAAAAGCTTGGAAAGTATGAACAAGTTTATTTTTCATCTGATGTCTCGTCAAAGAATATGTGCTTTTGCTGAAGGGAAAATCAATGACTTGGATGCATTTCAGATAGCAGTTACACCAGTGCCACAGAATGAGTTTTTGAGCAGTAAACTGACCGAAAAACTAGAACAGCAGATGCGGGATGGTTTGGCTGTGTCCGTTGGTGGTATGCCCCTGTGGTGTAATCAATTAATGGCATCGTGTCCTTTTTTGTTTAGTTTTGAGGTCAAATGCAAGTATTTCCGATTGGCAGCCTTTGTTCCACTACTAGGTCAGCCTCCTTCACCGTCTCACAGTGATTCAGGTATGACGAGTGACAGGCGACAAAGCTCTGGTGGCTTACCTCGACAAAAGTTTTTAGTTTTCCGCAACCGCATTCTGGATTCTGCTGCCCAAATGATGGAGCTGCATGCCTGTCAGAAGGTACTTCTTGAGGTGGAATATGATGAAGAAGTTGGTACTGGTCTTGGTCCAACATTGGAATTTTATACCCTGGTTAGTCATGAGTTTCAGAAATCTGGCCTGGGCATGTGGAGAGAGGATGGTGGATCTTTTACCTCTGGTACAAGCCATGCTGAGGATACTGGAATTTTGATAAGTCCTTGTGGTCTCTTTCCTCGTCCGTGGTCATCTACTATGGATGCATCAGATGGGACACAGTTCAGTGAAGTAATCAAAAAGTTTGCCCTTTTGGGGAAAATTGTAGGGAAATCGCTTCAGGATGGGAGGGTCTTGGATCTGCATTTCTCCAAAGCCTTTTATAAACTAATTCTTGGACAGGACCTTGGTTTGTATGACATCCAGTCATTTGATCCTGTGCTTGGAAGGACACTACTAGAGTTCAAGGCTCTTGTGGAAAGGAAAAGGTTTTTGGAATCTGTACATGGAGAAAATACAACATTTGAGTTTGACTTGTGCTTCAGGAAAACTAGAATTGAGGATCTTTGCCTTGATTTTACGCTTCCTGGGTATCCTGATTTTGTCCTTGCATTCGGGTTTGATCATAAAATGGTAAATTCCACGAACTTGGAAGATTATGTTTCACTTATGGCAGATGCAACTATAAATTCTGGAATTTCCAGACAAGTGGAAGCTTTCAAGTCTGGTTTTAACCAGGTGTTCCCCATTGAGCATCTTCAGATTTTCACTGAAGAAGAACTAGAGCGTCTGCTATGTGGAGAACATGATTCTTGGGCTGTATGTTTACTACCACTTAATCTTGATTGTTTTAAGTACTACCAAGTATATTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

HECT

Protein Analysis

1471

Amino Acids

164.47

Weight (kDa)

4.91

Isoelectric Point (pI)

50.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_TRIP12_N	PF25579	4 - 228	3.8e-102	E3 ubiquitin-protein ligase TRIP12-like, TPR repeats
HECT	PF00632	1220 - 1454	1.4e-35	HECT-domain (ubiquitin-transferase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011401)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02880 AT5G02880
fragaria_vesca	FvH4_6g15270 FvH4_6g15270 FvH4_6g15270 FvH4_6g15270
malus_domestica	MD04G1104600.v1.1 MD12G1124500.v1.1
prunus_persica	Prupe.6G241700_v2.0.a1 Prupe.6G241700_v2.0.a1
pyrus_communis	pycom12g12310
rosa_chinensis	RchiOBHm_Chr3g0468671
rosa_laevigata	RLG00000024416
rosa_multiflora	Rmu_sc0011538.1_g000007
rosa_roxburghii	Rroxscaffold_6G00412660
rosa_rugosa	Rorug03G0096200
rosa_samantha	Rh3AG145000 Rh3BG167500 Rh3DG166300
rosa_wichuraiana	Rw3G013670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3924
AasI	GACNNNNNNGTC	1 cut(s)	2077
Acc36I	ACCTGC	1 cut(s)	2217
AccB1I	GGYRCC	1 cut(s)	1346
AccB7I	CCANNNNNTGG	3 cut(s)	2573, 2666, 3562
AccI	GTMKAC	4 cut(s)	634, 2928, 3029, 3038
AccII	CGCG	1 cut(s)	485
AciI	CCGC	8 cut(s)	22, 286, 485, 2491, 2595, 3301, 3529, 3535
AclWI	GGATC	9 cut(s)	845, 858, 1478, 1491, 2186, 3718, 3906, 3968, 4110
AcoI	YGGCCR	2 cut(s)	13, 48
AcsI	RAATTY	8 cut(s)	1339, 2052, 2263, 3644, 3750, 4177, 4228, 4236
AfaI	GTAC	9 cut(s)	91, 406, 554, 1835, 3588, 3622, 3727, 4044, 4397
AflII	CTTAAG	2 cut(s)	1433, 2278
AflIII	ACRYGT	2 cut(s)	1929, 3057
AgeI	ACCGGT	1 cut(s)	2088
AhdI	GACNNNNNGTC	1 cut(s)	3964
AhlI	ACTAGT	1 cut(s)	1621
AjiI	CACGTC	1 cut(s)	1932
AjnI	CCWGG	6 cut(s)	363, 2680, 3654, 3684, 4126, 4275
AjuI	GAANNNNNNNTTGG	8 cut(s)	1919, 1951, 2649, 2681, 2886, 2918, 4016, 4048
Alw21I	GWGCWC	5 cut(s)	131, 1409, 1951, 2359, 2971
AlwI	GGATC	9 cut(s)	845, 858, 1478, 1491, 2186, 3718, 3906, 3968, 4110
AlwNI	CAGNNNCTG	2 cut(s)	364, 578
AoxI	GGCC	6 cut(s)	13, 48, 60, 1280, 2462, 3682
ApeKI	GCWGC	6 cut(s)	1095, 1678, 2120, 3413, 3551, 3568
ApoI	RAATTY	8 cut(s)	1339, 2052, 2263, 3644, 3750, 4177, 4228, 4236
ArsI	GACNNNNNNTTYG	2 cut(s)	428, 460
AseI	ATTAAT	1 cut(s)	3353
AsiGI	ACCGGT	1 cut(s)	2088
Asp700I	GAANNNNTTC	1 cut(s)	1900
AspLEI	GCGC	1 cut(s)	485
AspS9I	GGNCC	6 cut(s)	60, 559, 794, 1717, 3632, 3943
AsuII	TTCGAA	1 cut(s)	1643
AvaII	GGWCC	5 cut(s)	559, 794, 1717, 3632, 3943
BalI	TGGCCA	1 cut(s)	15
BamHI	GGATCC	2 cut(s)	850, 1483
BanI	GGYRCC	1 cut(s)	1346
BanII	GRGCYC	2 cut(s)	131, 2359
BbsI	GAAGAC	3 cut(s)	906, 1302, 2754
Bbv12I	GWGCWC	5 cut(s)	131, 1409, 1951, 2359, 2971
BbvCI	CCTCAGC	1 cut(s)	3738
BbvI	GCAGC	6 cut(s)	1107, 1665, 2107, 3425, 3538, 3555
BccI	CCATC	9 cut(s)	1157, 1765, 2542, 2752, 3299, 3556, 3698, 3806, 3878
BcgI	CGANNNNNNTGC	2 cut(s)	854, 888
BciT130I	CCWGG	6 cut(s)	365, 2682, 3656, 3686, 4128, 4277
BciVI	GTATCC	4 cut(s)	175, 1933, 3736, 4143
BclI	TGATCA	5 cut(s)	148, 163, 172, 301, 4162
BcuI	ACTAGT	1 cut(s)	1621
BfmI	CTRYAG	4 cut(s)	64, 2142, 2313, 2952
BfrI	CTTAAG	2 cut(s)	1433, 2278
BfuAI	ACCTGC	1 cut(s)	2217
BfuI	GTATCC	4 cut(s)	175, 1933, 3736, 4143
BisI	GCNGC	7 cut(s)	1096, 1679, 2121, 2491, 3414, 3552, 3569
BlpI	GCTNAGC	1 cut(s)	624
BlsI	GCNGC	7 cut(s)	1097, 1680, 2122, 2492, 3415, 3553, 3570
BmcAI	AGTACT	2 cut(s)	1835, 4397
Bme1390I	CCNGG	6 cut(s)	365, 2682, 3656, 3686, 4128, 4277
Bme18I	GGWCC	5 cut(s)	559, 794, 1717, 3632, 3943
BmeRI	GACNNNNNGTC	1 cut(s)	3964
BmgBI	CACGTC	1 cut(s)	1932
BmgT120I	GGNCC	6 cut(s)	60, 559, 794, 1717, 3632, 3943
BmiI	GGNNCC	5 cut(s)	852, 1348, 1485, 2485, 2646
BmrFI	CCNGG	6 cut(s)	365, 2682, 3656, 3686, 4128, 4277
BoxI	GACNNNNGTC	1 cut(s)	1692
BpiI	GAAGAC	3 cut(s)	906, 1302, 2754
BpmI	CTGGAG	2 cut(s)	708, 1485
Bpu10I	CCTNAGC	1 cut(s)	3738
Bpu1102I	GCTNAGC	1 cut(s)	624
Bpu14I	TTCGAA	1 cut(s)	1643
BpuEI	CTTGAG	1 cut(s)	3614
BsaBI	GATNNNNATC	1 cut(s)	534
BsaI	GGTCTC	1 cut(s)	3776
BsaJI	CCNNGG	7 cut(s)	1935, 2389, 3654, 3685, 3785, 3946, 4127
BsaWI	WCCGGW	4 cut(s)	296, 1049, 1343, 2088
BsaXI	ACNNNNNCTCC	4 cut(s)	638, 668, 1933, 1963
Bse118I	RCCGGY	2 cut(s)	46, 2088
Bse1I	ACTGG	6 cut(s)	1658, 2578, 3242, 3628, 3751, 3964
Bse3DI	GCAATG	5 cut(s)	151, 519, 635, 1168, 2794
Bse8I	GATNNNNATC	1 cut(s)	534
BseBI	CCWGG	6 cut(s)	365, 2682, 3656, 3686, 4128, 4277
BseDI	CCNNGG	7 cut(s)	1935, 2389, 3654, 3685, 3785, 3946, 4127
BseJI	GATNNNNATC	1 cut(s)	534
BseMI	GCAATG	5 cut(s)	151, 519, 635, 1168, 2794
BseNI	ACTGG	6 cut(s)	1658, 2578, 3242, 3628, 3751, 3964
BseRI	GAGGAG	1 cut(s)	213
BseX3I	CGGCCG	1 cut(s)	48
BseXI	GCAGC	6 cut(s)	1107, 1665, 2107, 3425, 3538, 3555
Bsh1236I	CGCG	1 cut(s)	485
Bsh1285I	CGRYCG	1 cut(s)	51
BshFI	GGCC	6 cut(s)	15, 50, 62, 1282, 2464, 3684
BshNI	GGYRCC	1 cut(s)	1346
BshTI	ACCGGT	1 cut(s)	2088
BsiEI	CGRYCG	1 cut(s)	51
BsiHKAI	GWGCWC	5 cut(s)	131, 1409, 1951, 2359, 2971
BsiSI	CCGG	6 cut(s)	47, 297, 1050, 1344, 2089, 2642
BslFI	GGGAC	3 cut(s)	287, 985, 3829
BsmBI	CGTCTC	3 cut(s)	108, 1326, 3456
BsmFI	GGGAC	3 cut(s)	287, 985, 3829
BsmI	GAATGC	4 cut(s)	1444, 2531, 3537, 4151
BsnI	GGCC	6 cut(s)	15, 50, 62, 1282, 2464, 3684
Bso31I	GGTCTC	1 cut(s)	3776
Bsp119I	TTCGAA	1 cut(s)	1643
Bsp1286I	GDGCHC	5 cut(s)	131, 1409, 1951, 2359, 2971
Bsp1407I	TGTACA	2 cut(s)	404, 4042
Bsp1720I	GCTNAGC	1 cut(s)	624
BspACI	CCGC	8 cut(s)	22, 286, 485, 2491, 2595, 3301, 3529, 3535
BspANI	GGCC	6 cut(s)	15, 50, 62, 1282, 2464, 3684
BspFNI	CGCG	1 cut(s)	485
BspHI	TCATGA	2 cut(s)	160, 3664
BspLI	GGNNCC	5 cut(s)	852, 1348, 1485, 2485, 2646
BspMI	ACCTGC	1 cut(s)	2217
BspPI	GGATC	9 cut(s)	845, 858, 1478, 1491, 2186, 3718, 3906, 3968, 4110
BspT104I	TTCGAA	1 cut(s)	1643
BspT107I	GGYRCC	1 cut(s)	1346
BspTI	CTTAAG	2 cut(s)	1433, 2278
BspTNI	GGTCTC	1 cut(s)	3776
BsrDI	GCAATG	5 cut(s)	151, 519, 635, 1168, 2794
BsrFI	RCCGGY	2 cut(s)	46, 2088
BsrGI	TGTACA	2 cut(s)	404, 4042
BsrI	ACTGG	6 cut(s)	1658, 2578, 3242, 3628, 3751, 3964
BssAI	RCCGGY	2 cut(s)	46, 2088
BssECI	CCNNGG	7 cut(s)	1935, 2389, 3654, 3685, 3785, 3946, 4127
BssNAI	GTATAC	3 cut(s)	2929, 3030, 3039
BssT1I	CCWWGG	3 cut(s)	1935, 2389, 3946
Bst1107I	GTATAC	3 cut(s)	2929, 3030, 3039
Bst2UI	CCWGG	6 cut(s)	365, 2682, 3656, 3686, 4128, 4277
Bst6I	CTCTTC	3 cut(s)	1959, 2008, 2373
BstAFI	CTTAAG	2 cut(s)	1433, 2278
BstAPI	GCANNNNNTGC	1 cut(s)	578
BstAUI	TGTACA	2 cut(s)	404, 4042
BstBI	TTCGAA	1 cut(s)	1643
BstC8I	GCNNGC	6 cut(s)	48, 676, 1784, 2545, 3075, 3573
BstDSI	CCRYGG	1 cut(s)	3785
BstEII	GGTNACC	1 cut(s)	2204
BstENI	CCTNNNNNAGG	1 cut(s)	789
BstFNI	CGCG	1 cut(s)	485
BstHHI	GCGC	1 cut(s)	485
BstMCI	CGRYCG	1 cut(s)	51
BstMWI	GCNNNNNNNGC	7 cut(s)	509, 578, 698, 984, 1371, 1592, 3498
BstNI	CCWGG	6 cut(s)	365, 2682, 3656, 3686, 4128, 4277
BstNSI	RCATGY	8 cut(s)	678, 1420, 1786, 1823, 2593, 3061, 3575, 3694
BstPAI	GACNNNNGTC	1 cut(s)	1692
BstPI	GGTNACC	1 cut(s)	2204
BstSCI	CCNGG	6 cut(s)	363, 2680, 3654, 3684, 4126, 4275
BstSFI	CTRYAG	4 cut(s)	64, 2142, 2313, 2952
BstUI	CGCG	1 cut(s)	485
BstV1I	GCAGC	6 cut(s)	1107, 1665, 2107, 3425, 3538, 3555
BstV2I	GAAGAC	3 cut(s)	906, 1302, 2754
BstX2I	RGATCY	6 cut(s)	850, 1483, 2191, 3710, 3898, 4102
BstXI	CCANNNNNNTGG	2 cut(s)	4189, 4249
BstYI	RGATCY	6 cut(s)	850, 1483, 2191, 3710, 3898, 4102
BstZ17I	GTATAC	3 cut(s)	2929, 3030, 3039
BstZI	CGGCCG	1 cut(s)	48
BsuI	GTATCC	4 cut(s)	175, 1933, 3736, 4143
BsuRI	GGCC	6 cut(s)	15, 50, 62, 1282, 2464, 3684
BtgI	CCRYGG	1 cut(s)	3785
BtrI	CACGTC	1 cut(s)	1932
BtsI	GCAGTG	2 cut(s)	579, 2334
BveI	ACCTGC	1 cut(s)	2217
Cac8I	GCNNGC	6 cut(s)	48, 676, 1784, 2545, 3075, 3573
CaiI	CAGNNNCTG	2 cut(s)	364, 578
CciI	TCATGA	2 cut(s)	160, 3664
CfoI	GCGC	1 cut(s)	485
Cfr10I	RCCGGY	2 cut(s)	46, 2088
Cfr13I	GGNCC	6 cut(s)	60, 559, 794, 1717, 3632, 3943
CseI	GACGC	2 cut(s)	821, 4316
CsiI	ACCWGGT	2 cut(s)	363, 4275
Csp6I	GTAC	9 cut(s)	90, 405, 553, 1834, 3587, 3621, 3726, 4043, 4396
CspAI	ACCGGT	1 cut(s)	2088
CviQI	GTAC	9 cut(s)	90, 405, 553, 1834, 3587, 3621, 3726, 4043, 4396
DrdI	GACNNNNNNGTC	1 cut(s)	2077
DriI	GACNNNNNGTC	1 cut(s)	3964
DseDI	GACNNNNNNGTC	1 cut(s)	2077
EaeI	YGGCCR	2 cut(s)	13, 48
EagI	CGGCCG	1 cut(s)	48
Eam1104I	CTCTTC	3 cut(s)	1959, 2008, 2373
Eam1105I	GACNNNNNGTC	1 cut(s)	3964
EarI	CTCTTC	3 cut(s)	1959, 2008, 2373
EciI	GGCGGA	1 cut(s)	301
Ecl136II	GAGCTC	2 cut(s)	129, 2357
EclXI	CGGCCG	1 cut(s)	48
Eco130I	CCWWGG	3 cut(s)	1935, 2389, 3946
Eco24I	GRGCYC	2 cut(s)	131, 2359
Eco31I	GGTCTC	1 cut(s)	3776
Eco32I	GATATC	2 cut(s)	490, 2134
Eco47I	GGWCC	5 cut(s)	559, 794, 1717, 3632, 3943
Eco52I	CGGCCG	1 cut(s)	48
Eco53kI	GAGCTC	2 cut(s)	129, 2357
Eco91I	GGTNACC	1 cut(s)	2204
EcoICRI	GAGCTC	2 cut(s)	129, 2357
EcoNI	CCTNNNNNAGG	1 cut(s)	789
EcoO109I	RGGNCCY	4 cut(s)	60, 794, 1717, 3943
EcoO65I	GGTNACC	1 cut(s)	2204
EcoRI	GAATTC	1 cut(s)	2052
EcoRII	CCWGG	6 cut(s)	363, 2680, 3654, 3684, 4126, 4275
EcoRV	GATATC	2 cut(s)	490, 2134
EcoT14I	CCWWGG	3 cut(s)	1935, 2389, 3946
EcoT22I	ATGCAT	3 cut(s)	1989, 3223, 3808
EcoT38I	GRGCYC	2 cut(s)	131, 2359
ErhI	CCWWGG	3 cut(s)	1935, 2389, 3946
Esp3I	CGTCTC	3 cut(s)	108, 1326, 3456
FalI	AAGNNNNNCTT	2 cut(s)	336, 368
FaqI	GGGAC	3 cut(s)	287, 985, 3829
FauI	CCCGC	1 cut(s)	3294
FauNDI	CATATG	3 cut(s)	177, 2308, 2323
FbaI	TGATCA	5 cut(s)	148, 163, 172, 301, 4162
FblI	GTMKAC	4 cut(s)	634, 2928, 3029, 3038
Fnu4HI	GCNGC	7 cut(s)	1096, 1679, 2121, 2491, 3414, 3552, 3569
FriOI	GRGCYC	2 cut(s)	131, 2359
Fsp4HI	GCNGC	7 cut(s)	1096, 1679, 2121, 2491, 3414, 3552, 3569
GlaI	GCGC	1 cut(s)	484
GluI	GCNGC	7 cut(s)	1096, 1679, 2121, 2491, 3414, 3552, 3569
GsuI	CTGGAG	2 cut(s)	708, 1485
HaeIII	GGCC	6 cut(s)	15, 50, 62, 1282, 2464, 3684
HapII	CCGG	6 cut(s)	47, 297, 1050, 1344, 2089, 2642
HgaI	GACGC	2 cut(s)	821, 4316
HhaI	GCGC	1 cut(s)	485
Hin6I	GCGC	1 cut(s)	483
HinP1I	GCGC	1 cut(s)	483
HincII	GTYRAC	2 cut(s)	2422, 2986
HindII	GTYRAC	2 cut(s)	2422, 2986
HindIII	AAGCTT	4 cut(s)	1889, 2003, 3130, 4254
HpaII	CCGG	6 cut(s)	47, 297, 1050, 1344, 2089, 2642
HpyCH4IV	ACGT	2 cut(s)	1931, 3032
HpyF10VI	GCNNNNNNNGC	7 cut(s)	509, 578, 698, 984, 1371, 1592, 3498
HpySE526I	ACGT	2 cut(s)	1931, 3032
HspAI	GCGC	1 cut(s)	483
KroI	GCCGGC	1 cut(s)	46
KroNI	GCCGGC	1 cut(s)	48
Ksp22I	TGATCA	5 cut(s)	148, 163, 172, 301, 4162
LmnI	GCTCC	6 cut(s)	134, 661, 689, 1246, 1954, 3565
Lsp1109I	GCAGC	6 cut(s)	1107, 1665, 2107, 3425, 3538, 3555
MabI	ACCWGGT	2 cut(s)	363, 4275
MaeII	ACGT	2 cut(s)	1931, 3032
MaeIII	GTNAC	9 cut(s)	444, 654, 1197, 1624, 1853, 2204, 2218, 3235, 3476
MfeI	CAATTG	1 cut(s)	2508
MflI	RGATCY	6 cut(s)	850, 1483, 2191, 3710, 3898, 4102
MhlI	GDGCHC	5 cut(s)	131, 1409, 1951, 2359, 2971
MlsI	TGGCCA	1 cut(s)	15
MluNI	TGGCCA	1 cut(s)	15
MlyI	GAGTC	6 cut(s)	916, 1066, 1476, 1550, 1682, 2981
MmeI	TCCRAC	3 cut(s)	586, 3078, 3659
Mox20I	TGGCCA	1 cut(s)	15
Mph1103I	ATGCAT	3 cut(s)	1989, 3223, 3808
MroNI	GCCGGC	1 cut(s)	46
MroXI	GAANNNNTTC	1 cut(s)	1900
MscI	TGGCCA	1 cut(s)	15
MslI	CAYNNNNRTG	4 cut(s)	165, 673, 2344, 2756
Msp20I	TGGCCA	1 cut(s)	15
MspA1I	CMGCKG	1 cut(s)	2836
MspCI	CTTAAG	2 cut(s)	1433, 2278
MspI	CCGG	6 cut(s)	47, 297, 1050, 1344, 2089, 2642
MspR9I	CCNGG	6 cut(s)	365, 2682, 3656, 3686, 4128, 4277
MunI	CAATTG	1 cut(s)	2508
Mva1269I	GAATGC	4 cut(s)	1444, 2531, 3537, 4151
MvaI	CCWGG	6 cut(s)	365, 2682, 3656, 3686, 4128, 4277
MvnI	CGCG	1 cut(s)	485
MwoI	GCNNNNNNNGC	7 cut(s)	509, 578, 698, 984, 1371, 1592, 3498
NaeI	GCCGGC	1 cut(s)	48
NdeI	CATATG	3 cut(s)	177, 2308, 2323
NgoMIV	GCCGGC	1 cut(s)	46
NlaIV	GGNNCC	5 cut(s)	852, 1348, 1485, 2485, 2646
NmeAIII	GCCGAG	1 cut(s)	2969
NmuCI	GTSAC	5 cut(s)	444, 654, 1853, 2204, 3476
NsiI	ATGCAT	3 cut(s)	1989, 3223, 3808
NspI	RCATGY	8 cut(s)	678, 1420, 1786, 1823, 2593, 3061, 3575, 3694
NspV	TTCGAA	1 cut(s)	1643
PaeI	GCATGC	3 cut(s)	678, 1786, 3575
PagI	TCATGA	2 cut(s)	160, 3664
PciI	ACATGT	1 cut(s)	3057
PcsI	WCGNNNNNNNCGW	2 cut(s)	446, 2192
PctI	GAATGC	4 cut(s)	1444, 2531, 3537, 4151
PdiI	GCCGGC	1 cut(s)	48
PdmI	GAANNNNTTC	1 cut(s)	1900
PflMI	CCANNNNNTGG	3 cut(s)	2573, 2666, 3562
PfoI	TCCNGGA	1 cut(s)	2680
PinAI	ACCGGT	1 cut(s)	2088
PkrI	GCNGC	7 cut(s)	1097, 1680, 2122, 2492, 3415, 3553, 3570
PleI	GAGTC	6 cut(s)	915, 1066, 1475, 1550, 1682, 2981
PpsI	GAGTC	6 cut(s)	915, 1066, 1475, 1550, 1682, 2981
PpuMI	RGGWCCY	3 cut(s)	794, 1717, 3943
PscI	ACATGT	1 cut(s)	3057
PshAI	GACNNNNGTC	1 cut(s)	1692
PshBI	ATTAAT	1 cut(s)	3353
PsiI	TTATAA	1 cut(s)	3924
Psp124BI	GAGCTC	2 cut(s)	131, 2359
Psp5II	RGGWCCY	3 cut(s)	794, 1717, 3943
Psp6I	CCWGG	6 cut(s)	363, 2680, 3654, 3684, 4126, 4275
PspEI	GGTNACC	1 cut(s)	2204
PspGI	CCWGG	6 cut(s)	363, 2680, 3654, 3684, 4126, 4275
PspN4I	GGNNCC	5 cut(s)	852, 1348, 1485, 2485, 2646
PspPI	GGNCC	6 cut(s)	60, 559, 794, 1717, 3632, 3943
PspPPI	RGGWCCY	3 cut(s)	794, 1717, 3943
PsrI	GAACNNNNNNTAC	2 cut(s)	1173, 1205
PstNI	CAGNNNCTG	2 cut(s)	364, 578
PsuI	RGATCY	6 cut(s)	850, 1483, 2191, 3710, 3898, 4102
PvuII	CAGCTG	1 cut(s)	2836
RsaI	GTAC	9 cut(s)	91, 406, 554, 1835, 3588, 3622, 3727, 4044, 4397
RsaNI	GTAC	9 cut(s)	90, 405, 553, 1834, 3587, 3621, 3726, 4043, 4396
RseI	CAYNNNNRTG	4 cut(s)	165, 673, 2344, 2756
SacI	GAGCTC	2 cut(s)	131, 2359
SatI	GCNGC	7 cut(s)	1096, 1679, 2121, 2491, 3414, 3552, 3569
Sau96I	GGNCC	6 cut(s)	60, 559, 794, 1717, 3632, 3943
ScaI	AGTACT	2 cut(s)	1835, 4397
SchI	GAGTC	6 cut(s)	916, 1066, 1476, 1550, 1682, 2981
ScrFI	CCNGG	6 cut(s)	365, 2682, 3656, 3686, 4128, 4277
SduI	GDGCHC	5 cut(s)	131, 1409, 1951, 2359, 2971
SexAI	ACCWGGT	2 cut(s)	363, 4275
SfcI	CTRYAG	4 cut(s)	64, 2142, 2313, 2952
SfuI	TTCGAA	1 cut(s)	1643
SinI	GGWCC	5 cut(s)	559, 794, 1717, 3632, 3943
SmiMI	CAYNNNNRTG	4 cut(s)	165, 673, 2344, 2756
SmlI	CTYRAG	3 cut(s)	1433, 2278, 3593
SmoI	CTYRAG	3 cut(s)	1433, 2278, 3593
SpeI	ACTAGT	1 cut(s)	1621
SphI	GCATGC	3 cut(s)	678, 1786, 3575
SsiI	CCGC	8 cut(s)	22, 286, 485, 2491, 2595, 3301, 3529, 3535
SstI	GAGCTC	2 cut(s)	131, 2359
StyD4I	CCNGG	6 cut(s)	363, 2680, 3654, 3684, 4126, 4275
StyI	CCWWGG	3 cut(s)	1935, 2389, 3946
TaiI	ACGT	2 cut(s)	1934, 3035
TaqI	TCGA	6 cut(s)	348, 434, 864, 1477, 1643, 3508
TaqII	GACCGA	2 cut(s)	100, 3293
TatI	WGTACW	6 cut(s)	89, 404, 552, 1833, 4042, 4395
TauI	GCSGC	1 cut(s)	2493
TseFI	GTSAC	5 cut(s)	444, 654, 1853, 2204, 3476
TseI	GCWGC	6 cut(s)	1095, 1678, 2120, 3413, 3551, 3568
Tsp45I	GTSAC	5 cut(s)	444, 654, 1853, 2204, 3476
TspGWI	ACGGA	6 cut(s)	1308, 1525, 2184, 2245, 3310, 3774
Van91I	CCANNNNNTGG	3 cut(s)	2573, 2666, 3562
Vha464I	CTTAAG	2 cut(s)	1433, 2278
VpaK11BI	GGWCC	5 cut(s)	559, 794, 1717, 3632, 3943
VspI	ATTAAT	1 cut(s)	3353
XagI	CCTNNNNNAGG	1 cut(s)	789
XapI	RAATTY	8 cut(s)	1339, 2052, 2263, 3644, 3750, 4177, 4228, 4236
XceI	RCATGY	8 cut(s)	678, 1420, 1786, 1823, 2593, 3061, 3575, 3694
XmiI	GTMKAC	4 cut(s)	634, 2928, 3029, 3038
XmnI	GAANNNNTTC	1 cut(s)	1900
ZrmI	AGTACT	2 cut(s)	1835, 4397
Zsp2I	ATGCAT	3 cut(s)	1989, 3223, 3808

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.