MD04G1119000.v1.1

Plant protein 1589 of unknown function (A_thal_3526)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009837
Physical Location & Seq
Reverse (-)
20531398 .. 20532821
1424 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1119000.v1.1.491

Sequence Viewer

Length: 324 bp
ATGGGAGACTCTTCTGCTTCATACATCCACTTGGTGCACCATTTAATCGAGGAGTGCATCATATTCAACATGAGCAAAGAGGAGTGCATGGAAACCCTCTCTAAGCATGCAAACATAAAACCCATCATTACCTCCACAGTGTGGAAGGAGTTAGAGAAGGAGAACAGAGAGTTCTTTGAGGCCTACACAAAGAGCAGGGAAGCAGGATCCTCTGAGATGGTCACAAAGCAAAGAATCCAGAAGATGCTTTTTGATCTTACCCAGAGCGGCGACAACTCCACCACCACCGACGACGACGGTGATGAATCTGAATCTGATGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
A_thal_3526

Protein Analysis

108

Amino Acids

12.24

Weight (kDa)

4.61

Isoelectric Point (pI)

61.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
A_thal_3526 PF09713 12 - 63 1.2e-26 Plant protein 1589 of unknown function (A_thal_3526)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 141
AccBSI CCGCTC 1 cut(s) 267
AciI CCGC 1 cut(s) 267
AclWI GGATC 2 cut(s) 201, 214
AdeI CACNNNGTG 2 cut(s) 34, 141
AfiI CCNNNNNNNGG 1 cut(s) 141
AgsI TTSAA 1 cut(s) 67
Alw21I GWGCWC 1 cut(s) 39
Alw44I GTGCAC 1 cut(s) 35
AlwI GGATC 2 cut(s) 201, 214
AoxI GGCC 1 cut(s) 180
ApaLI GTGCAC 1 cut(s) 35
AsuHPI GGTGA 1 cut(s) 311
BaeGI GKGCMC 1 cut(s) 39
BamHI GGATCC 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 39
BccI CCATC 2 cut(s) 131, 211
BfaI CTAG 1 cut(s) 322
BisI GCNGC 1 cut(s) 268
BlsI GCNGC 1 cut(s) 269
BmiI GGNNCC 1 cut(s) 208
BmsI GCATC 2 cut(s) 66, 234
Bsc4I CCNNNNNNNGG 1 cut(s) 141
BseGI GGATG 1 cut(s) 24
BseLI CCNNNNNNNGG 1 cut(s) 141
BseMII CTCAG 1 cut(s) 204
BseRI GAGGAG 2 cut(s) 65, 95
BseSI GKGCMC 1 cut(s) 39
BshFI GGCC 1 cut(s) 182
BsiHKAI GWGCWC 1 cut(s) 39
BslI CCNNNNNNNGG 1 cut(s) 141
BsnI GGCC 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 2 cut(s) 206, 253
BspACI CCGC 1 cut(s) 267
BspANI GGCC 1 cut(s) 182
BspCNI CTCAG 1 cut(s) 205
BspLI GGNNCC 1 cut(s) 208
BspPI GGATC 2 cut(s) 201, 214
BsrBI CCGCTC 1 cut(s) 267
BssMI GATC 2 cut(s) 206, 253
Bst4CI ACNGT 2 cut(s) 139, 299
Bst6I CTCTTC 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 2 cut(s) 102, 213
BstF5I GGATG 1 cut(s) 24
BstKTI GATC 2 cut(s) 209, 256
BstMBI GATC 2 cut(s) 206, 253
BstNSI RCATGY 1 cut(s) 110
BstSLI GKGCMC 1 cut(s) 39
BstX2I RGATCY 1 cut(s) 206
BstYI RGATCY 1 cut(s) 206
BsuRI GGCC 1 cut(s) 182
BtsCI GGATG 1 cut(s) 24
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 108
CviAII CATG 3 cut(s) 70, 88, 107
CviJI RGCY 1 cut(s) 182
CviKI_1 RGCY 1 cut(s) 182
DdeI CTNAG 2 cut(s) 102, 213
DpnI GATC 2 cut(s) 208, 255
DpnII GATC 2 cut(s) 206, 253
DraIII CACNNNGTG 2 cut(s) 34, 141
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Eco147I AGGCCT 1 cut(s) 182
FaeI CATG 3 cut(s) 73, 91, 110
FaiI YATR 6 cut(s) 22, 62, 71, 89, 108, 116
FatI CATG 3 cut(s) 69, 87, 106
Fnu4HI GCNGC 1 cut(s) 268
FokI GGATG 1 cut(s) 11
Fsp4HI GCNGC 1 cut(s) 268
FspBI CTAG 1 cut(s) 322
GluI GCNGC 1 cut(s) 268
HaeIII GGCC 1 cut(s) 182
Hin1II CATG 3 cut(s) 73, 91, 110
HinfI GANTC 4 cut(s) 8, 234, 305, 311
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 3 cut(s) 214, 310, 316
Hpy188III TCNNGA 1 cut(s) 238
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 3 cut(s) 293, 296, 299
HpyAV CCTTC 2 cut(s) 139, 151
HpyCH4III ACNGT 2 cut(s) 139, 299
HpyCH4V TGCA 4 cut(s) 37, 57, 87, 110
HpyF3I CTNAG 2 cut(s) 102, 213
Hsp92II CATG 3 cut(s) 73, 91, 110
Kzo9I GATC 2 cut(s) 206, 253
LpnPI CCDG 4 cut(s) 181, 189, 251, 275
LweI GCATC 2 cut(s) 66, 234
MaeI CTAG 1 cut(s) 322
MaeIII GTNAC 1 cut(s) 220
MalI GATC 2 cut(s) 208, 255
MbiI CCGCTC 1 cut(s) 267
MboI GATC 2 cut(s) 206, 253
MboII GAAGA 2 cut(s) 3, 253
MflI RGATCY 1 cut(s) 206
MhlI GDGCHC 1 cut(s) 39
MlyI GAGTC 1 cut(s) 2
MnlI CCTC 6 cut(s) 43, 73, 107, 142, 172, 220
MseI TTAA 1 cut(s) 44
NdeII GATC 2 cut(s) 206, 253
NlaIII CATG 3 cut(s) 73, 91, 110
NlaIV GGNNCC 1 cut(s) 208
NmuCI GTSAC 1 cut(s) 220
NspI RCATGY 1 cut(s) 110
PaeI GCATGC 1 cut(s) 110
PceI AGGCCT 1 cut(s) 182
PfeI GAWTC 3 cut(s) 234, 305, 311
PflMI CCANNNNNTGG 1 cut(s) 141
PkrI GCNGC 1 cut(s) 269
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
PspN4I GGNNCC 1 cut(s) 208
PsuI RGATCY 1 cut(s) 206
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 1 cut(s) 268
Sau3AI GATC 2 cut(s) 206, 253
SchI GAGTC 1 cut(s) 2
SduI GDGCHC 1 cut(s) 39
SetI ASST 1 cut(s) 134
SfaNI GCATC 2 cut(s) 66, 234
SgeI CNNG 9 cut(s) 43, 61, 82, 100, 119, 208, 216, 250, 274
SphI GCATGC 1 cut(s) 110
SseBI AGGCCT 1 cut(s) 182
SsiI CCGC 1 cut(s) 267
SspMI CTAG 1 cut(s) 322
StuI AGGCCT 1 cut(s) 182
TaaI ACNGT 2 cut(s) 139, 299
TaqI TCGA 1 cut(s) 48
TauI GCSGC 1 cut(s) 270
TfiI GAWTC 3 cut(s) 234, 305, 311
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TscAI CASTG 1 cut(s) 144
TseFI GTSAC 1 cut(s) 220
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 2 cut(s) 9, 318
TspRI CASTG 1 cut(s) 144
Van91I CCANNNNNTGG 1 cut(s) 141
VneI GTGCAC 1 cut(s) 35
XceI RCATGY 1 cut(s) 110
XspI CTAG 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.