Rorug03G0089100

Plant protein 1589 of unknown function (A_thal_3526)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0009837
Physical Location & Seq
Forward (+)
6950274 .. 6951709
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0089100.1

Sequence Viewer

Length: 966 bp
ATGGAGTGTCCTTGGCCTGAAAGTGTTTTCTCTTCGGAGAATCGGCAAAGGGCGATGGAAGAAGAGCTGGTTCGAGAAGAGGAATTTGCGAATCAACTTCGCGAGTTTCTTGATGATCATGGTGATCATGGTCGATGGGTGGTGGCCCAAGCTCTTGGTACGAAGATCTCGAGCTCTTTTACGAATTCGAATAGCCTTTCAATCTTAAAAGGGAATCATCAGGGGGAGCTAGTTCCTCATATTATTCGAGCCAACACTACTTTTGCTGCGCCTACTATTGTGAATTCATCGTCTTGGAATGCACAGGAGGGACGACATAGGGCTATTATGAGTACCGAAACGTCCAAGGACCGTAGAGGTTGTTATAAGAGAAGAAAGACTCCATATTCATGGACCGGAAACACTCCAGATTTGGTTAACGATGGTCATGCATGGAGAAAGAATGGACAGAAACAGATCCTCAATGCCACCCACCCAAGGTGCAGCCATAAGTACGACCAAGGCTGCAGAGCAACCAAGCAGGTGCAGAAAGTTGAAGATTATCCCTCTCTGTTTCGGACTGCATATTTTGGAAATCACACATGCACTTATCACTTCCTCAAAGCTCCTGAGCTCCTCCTGCAGGATGGTCTAAGTAGTACCCCTAGTCCTACAGAAGACTGTAATTCAAAGTTTACTATTACATTTGGCAACACAAACTACAGCCTCGCAAACCAACAAGAAGACCCTTTTTTCTCATCCAACATATCAACCAGAGAGGAGATGATCAACTCTAATCAAATAGCCACCAACCAATCATCAATATTTGATGATCTCGTGTCACTTGATCAACTGCCGGTGCTTGATTCATCTGGGGTCATCAGCAGGTTGCCATCAATATTGTCTGAGCTTGATCACGAGGATACGACGTACAGGACAGTGACGGGTTATGTGTATCATGATGGTATCTTCCCATATGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
A_thal_3526

Protein Analysis

321

Amino Acids

36.41

Weight (kDa)

5.74

Isoelectric Point (pI)

51.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 140 - 195 8.7e-16 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 366
AarI CACCTGC 1 cut(s) 511
Acc36I ACCTGC 2 cut(s) 511, 855
AccII CGCG 1 cut(s) 102
AclWI GGATC 1 cut(s) 451
AcsI RAATTY 4 cut(s) 83, 184, 283, 960
AfaI GTAC 5 cut(s) 160, 334, 494, 640, 911
AfiI CCNNNNNNNGG 2 cut(s) 477, 622
AgsI TTSAA 3 cut(s) 201, 536, 669
AluBI AGCT 7 cut(s) 67, 152, 174, 229, 605, 613, 889
AluI AGCT 7 cut(s) 67, 152, 174, 229, 605, 613, 889
Alw21I GWGCWC 2 cut(s) 176, 615
AlwI GGATC 1 cut(s) 451
Ama87I CYCGRG 1 cut(s) 169
AoxI GGCC 2 cut(s) 14, 144
ApeKI GCWGC 3 cut(s) 266, 483, 504
ApoI RAATTY 4 cut(s) 83, 184, 283, 960
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 3 cut(s) 145, 349, 393
AsuHPI GGTGA 1 cut(s) 134
AsuII TTCGAA 1 cut(s) 188
AvaI CYCGRG 1 cut(s) 169
AvaII GGWCC 2 cut(s) 349, 393
BanII GRGCYC 2 cut(s) 176, 615
BauI CACGAG 2 cut(s) 815, 896
BbsI GAAGAC 2 cut(s) 663, 729
Bbv12I GWGCWC 2 cut(s) 176, 615
BbvI GCAGC 3 cut(s) 253, 491, 495
BccI CCATC 6 cut(s) 49, 129, 416, 620, 880, 935
BcgI CGANNNNNNTGC 2 cut(s) 410, 444
BciVI GTATCC 1 cut(s) 895
BclI TGATCA 5 cut(s) 115, 124, 765, 826, 892
BfaI CTAG 2 cut(s) 230, 645
BfmI CTRYAG 4 cut(s) 505, 620, 651, 700
BfuAI ACCTGC 2 cut(s) 511, 855
BfuI GTATCC 1 cut(s) 895
BglII AGATCT 1 cut(s) 165
BisI GCNGC 3 cut(s) 267, 484, 505
BlsI GCNGC 3 cut(s) 268, 485, 506
Bme18I GGWCC 2 cut(s) 349, 393
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 3 cut(s) 145, 349, 393
BpiI GAAGAC 2 cut(s) 663, 729
BpmI CTGGAG 1 cut(s) 390
Bpu10I CCTNAGC 1 cut(s) 609
Bpu14I TTCGAA 1 cut(s) 188
BsaJI CCNNGG 4 cut(s) 11, 345, 476, 499
BsaWI WCCGGW 1 cut(s) 395
Bsc4I CCNNNNNNNGG 2 cut(s) 477, 622
Bse118I RCCGGY 1 cut(s) 835
BseDI CCNNGG 4 cut(s) 11, 345, 476, 499
BseGI GGATG 2 cut(s) 631, 737
BseLI CCNNNNNNNGG 2 cut(s) 477, 622
BseMII CTCAG 2 cut(s) 600, 876
BseRI GAGGAG 2 cut(s) 605, 773
BseXI GCAGC 3 cut(s) 253, 491, 495
BsgI GTGCAG 2 cut(s) 502, 545
Bsh1236I CGCG 1 cut(s) 102
BshFI GGCC 2 cut(s) 16, 146
BsiHKAI GWGCWC 2 cut(s) 176, 615
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 2 cut(s) 396, 836
BslFI GGGAC 1 cut(s) 324
BslI CCNNNNNNNGG 2 cut(s) 477, 622
BsmFI GGGAC 1 cut(s) 324
BsmI GAATGC 1 cut(s) 304
BsnI GGCC 2 cut(s) 16, 146
BsoBI CYCGRG 1 cut(s) 169
Bsp119I TTCGAA 1 cut(s) 188
Bsp1286I GDGCHC 2 cut(s) 176, 615
Bsp143I GATC 8 cut(s) 115, 124, 165, 456, 765, 811, 826, 892
Bsp68I TCGCGA 1 cut(s) 102
BspANI GGCC 2 cut(s) 16, 146
BspCNI CTCAG 2 cut(s) 601, 877
BspFNI CGCG 1 cut(s) 102
BspHI TCATGA 1 cut(s) 937
BspMAI CTGCAG 2 cut(s) 509, 624
BspMI ACCTGC 2 cut(s) 511, 855
BspPI GGATC 1 cut(s) 451
BspQI GCTCTTC 1 cut(s) 57
BspT104I TTCGAA 1 cut(s) 188
BsrFI RCCGGY 1 cut(s) 835
BssAI RCCGGY 1 cut(s) 835
BssECI CCNNGG 4 cut(s) 11, 345, 476, 499
BssMI GATC 8 cut(s) 115, 124, 165, 456, 765, 811, 826, 892
BssSI CACGAG 2 cut(s) 815, 896
BssT1I CCWWGG 4 cut(s) 11, 345, 476, 499
Bst2BI CACGAG 2 cut(s) 815, 896
Bst4CI ACNGT 3 cut(s) 353, 662, 919
Bst6I CTCTTC 3 cut(s) 37, 57, 72
BstBI TTCGAA 1 cut(s) 188
BstDEI CTNAG 3 cut(s) 609, 632, 885
BstENI CCTNNNNNAGG 1 cut(s) 620
BstF5I GGATG 2 cut(s) 631, 737
BstFNI CGCG 1 cut(s) 102
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 8 cut(s) 118, 127, 168, 459, 768, 814, 829, 895
BstMBI GATC 8 cut(s) 115, 124, 165, 456, 765, 811, 826, 892
BstMWI GCNNNNNNNGC 1 cut(s) 619
BstNSI RCATGY 1 cut(s) 585
BstSFI CTRYAG 4 cut(s) 505, 620, 651, 700
BstUI CGCG 1 cut(s) 102
BstV1I GCAGC 3 cut(s) 253, 491, 495
BstV2I GAAGAC 2 cut(s) 663, 729
BstX2I RGATCY 2 cut(s) 165, 456
BstXI CCANNNNNNTGG 2 cut(s) 155, 390
BstYI RGATCY 2 cut(s) 165, 456
BsuI GTATCC 1 cut(s) 895
BsuRI GGCC 2 cut(s) 16, 146
BtgZI GCGATG 1 cut(s) 68
BtsCI GGATG 2 cut(s) 631, 737
BtsIMutI CAGTG 1 cut(s) 924
BtuMI TCGCGA 1 cut(s) 102
BveI ACCTGC 2 cut(s) 511, 855
CciI TCATGA 1 cut(s) 937
CfoI GCGC 1 cut(s) 271
Cfr10I RCCGGY 1 cut(s) 835
Cfr13I GGNCC 3 cut(s) 145, 349, 393
Csp6I GTAC 5 cut(s) 159, 333, 493, 639, 910
CviAII CATG 7 cut(s) 119, 128, 390, 428, 432, 582, 938
CviQI GTAC 5 cut(s) 159, 333, 493, 639, 910
DdeI CTNAG 3 cut(s) 609, 632, 885
DpnI GATC 8 cut(s) 117, 126, 167, 458, 767, 813, 828, 894
DpnII GATC 8 cut(s) 115, 124, 165, 456, 765, 811, 826, 892
Eam1104I CTCTTC 3 cut(s) 37, 57, 72
EarI CTCTTC 3 cut(s) 37, 57, 72
Ecl136II GAGCTC 2 cut(s) 174, 613
Eco130I CCWWGG 4 cut(s) 11, 345, 476, 499
Eco24I GRGCYC 2 cut(s) 176, 615
Eco47I GGWCC 2 cut(s) 349, 393
Eco53kI GAGCTC 2 cut(s) 174, 613
Eco88I CYCGRG 1 cut(s) 169
EcoICRI GAGCTC 2 cut(s) 174, 613
EcoNI CCTNNNNNAGG 1 cut(s) 620
EcoRI GAATTC 2 cut(s) 184, 283
EcoT14I CCWWGG 4 cut(s) 11, 345, 476, 499
EcoT22I ATGCAT 1 cut(s) 433
EcoT38I GRGCYC 2 cut(s) 176, 615
ErhI CCWWGG 4 cut(s) 11, 345, 476, 499
FaeI CATG 7 cut(s) 122, 131, 393, 431, 435, 585, 941
FaqI GGGAC 1 cut(s) 324
FatI CATG 7 cut(s) 118, 127, 389, 427, 431, 581, 937
FauNDI CATATG 1 cut(s) 955
FbaI TGATCA 5 cut(s) 115, 124, 765, 826, 892
Fnu4HI GCNGC 3 cut(s) 267, 484, 505
FokI GGATG 2 cut(s) 638, 724
FriOI GRGCYC 2 cut(s) 176, 615
Fsp4HI GCNGC 3 cut(s) 267, 484, 505
FspBI CTAG 2 cut(s) 230, 645
GlaI GCGC 1 cut(s) 270
GluI GCNGC 3 cut(s) 267, 484, 505
GsuI CTGGAG 1 cut(s) 390
HaeIII GGCC 2 cut(s) 16, 146
HapII CCGG 2 cut(s) 396, 836
HhaI GCGC 1 cut(s) 271
Hin1II CATG 7 cut(s) 122, 131, 393, 431, 435, 585, 941
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 5 cut(s) 40, 91, 214, 379, 845
HpaI GTTAAC 1 cut(s) 418
HpaII CCGG 2 cut(s) 396, 836
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 2 cut(s) 418, 675
Hpy188I TCNGA 3 cut(s) 37, 558, 886
Hpy188III TCNNGA 8 cut(s) 74, 101, 110, 169, 407, 608, 896, 938
Hpy8I GTNNAC 2 cut(s) 418, 675
Hpy99I CGWCG 1 cut(s) 910
HpyCH4III ACNGT 3 cut(s) 353, 662, 919
HpyCH4IV ACGT 2 cut(s) 341, 908
HpyCH4V TGCA 8 cut(s) 302, 431, 483, 507, 526, 563, 585, 622
HpyF10VI GCNNNNNNNGC 1 cut(s) 619
HpyF3I CTNAG 3 cut(s) 609, 632, 885
HpySE526I ACGT 2 cut(s) 341, 908
Hsp92II CATG 7 cut(s) 122, 131, 393, 431, 435, 585, 941
HspAI GCGC 1 cut(s) 269
Ksp22I TGATCA 5 cut(s) 115, 124, 765, 826, 892
KspAI GTTAAC 1 cut(s) 418
Kzo9I GATC 8 cut(s) 115, 124, 165, 456, 765, 811, 826, 892
LguI GCTCTTC 1 cut(s) 57
LmnI GCTCC 3 cut(s) 226, 610, 618
Lsp1109I GCAGC 3 cut(s) 253, 491, 495
MaeI CTAG 2 cut(s) 230, 645
MaeII ACGT 2 cut(s) 341, 908
MaeIII GTNAC 2 cut(s) 819, 919
MalI GATC 8 cut(s) 117, 126, 167, 458, 767, 813, 828, 894
MboI GATC 8 cut(s) 115, 124, 165, 456, 765, 811, 826, 892
MflI RGATCY 2 cut(s) 165, 456
MhlI GDGCHC 2 cut(s) 176, 615
MluCI AATT 5 cut(s) 83, 184, 283, 664, 960
MlyI GAGTC 1 cut(s) 373
MmeI TCCRAC 1 cut(s) 765
Mph1103I ATGCAT 1 cut(s) 433
MseI TTAA 2 cut(s) 206, 417
MslI CAYNNNNRTG 1 cut(s) 388
MspI CCGG 2 cut(s) 396, 836
Mva1269I GAATGC 1 cut(s) 304
MvnI CGCG 1 cut(s) 102
MwoI GCNNNNNNNGC 1 cut(s) 619
NdeI CATATG 1 cut(s) 955
NdeII GATC 8 cut(s) 115, 124, 165, 456, 765, 811, 826, 892
NlaIII CATG 7 cut(s) 122, 131, 393, 431, 435, 585, 941
NmuCI GTSAC 2 cut(s) 819, 919
NruI TCGCGA 1 cut(s) 102
NsiI ATGCAT 1 cut(s) 433
NspI RCATGY 1 cut(s) 585
NspV TTCGAA 1 cut(s) 188
PaeR7I CTCGAG 1 cut(s) 169
PagI TCATGA 1 cut(s) 937
PaqCI CACCTGC 1 cut(s) 511
PciSI GCTCTTC 1 cut(s) 57
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PctI GAATGC 1 cut(s) 304
PfeI GAWTC 4 cut(s) 40, 91, 214, 845
PkrI GCNGC 3 cut(s) 268, 485, 506
PleI GAGTC 1 cut(s) 373
PpsI GAGTC 1 cut(s) 373
PsiI TTATAA 1 cut(s) 366
Psp124BI GAGCTC 2 cut(s) 176, 615
PspPI GGNCC 3 cut(s) 145, 349, 393
PstI CTGCAG 2 cut(s) 509, 624
PsuI RGATCY 2 cut(s) 165, 456
RruI TCGCGA 1 cut(s) 102
RsaI GTAC 5 cut(s) 160, 334, 494, 640, 911
RsaNI GTAC 5 cut(s) 159, 333, 493, 639, 910
RseI CAYNNNNRTG 1 cut(s) 388
SacI GAGCTC 2 cut(s) 176, 615
SapI GCTCTTC 1 cut(s) 57
SaqAI TTAA 2 cut(s) 206, 417
SatI GCNGC 3 cut(s) 267, 484, 505
Sau3AI GATC 8 cut(s) 115, 124, 165, 456, 765, 811, 826, 892
Sau96I GGNCC 3 cut(s) 145, 349, 393
SbfI CCTGCAGG 1 cut(s) 624
SchI GAGTC 1 cut(s) 373
SdaI CCTGCAGG 1 cut(s) 624
SduI GDGCHC 2 cut(s) 176, 615
SfcI CTRYAG 4 cut(s) 505, 620, 651, 700
Sfr274I CTCGAG 1 cut(s) 169
SfuI TTCGAA 1 cut(s) 188
SinI GGWCC 2 cut(s) 349, 393
SlaI CTCGAG 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 388
SmlI CTYRAG 1 cut(s) 169
SmoI CTYRAG 1 cut(s) 169
Sse8387I CCTGCAGG 1 cut(s) 624
Sse9I AATT 5 cut(s) 83, 184, 283, 664, 960
SspI AATATT 2 cut(s) 804, 879
SspMI CTAG 2 cut(s) 230, 645
SstI GAGCTC 2 cut(s) 176, 615
StyI CCWWGG 4 cut(s) 11, 345, 476, 499
TaaI ACNGT 3 cut(s) 353, 662, 919
TaiI ACGT 2 cut(s) 344, 911
TaqI TCGA 5 cut(s) 73, 133, 170, 188, 247
TasI AATT 5 cut(s) 83, 184, 283, 664, 960
TfiI GAWTC 4 cut(s) 40, 91, 214, 845
Tru1I TTAA 2 cut(s) 206, 417
Tru9I TTAA 2 cut(s) 206, 417
TscAI CASTG 1 cut(s) 924
TseFI GTSAC 2 cut(s) 819, 919
TseI GCWGC 3 cut(s) 266, 483, 504
Tsp45I GTSAC 2 cut(s) 819, 919
TspDTI ATGAA 3 cut(s) 276, 378, 837
TspRI CASTG 1 cut(s) 924
VpaK11BI GGWCC 2 cut(s) 349, 393
XagI CCTNNNNNAGG 1 cut(s) 620
XapI RAATTY 4 cut(s) 83, 184, 283, 960
XceI RCATGY 1 cut(s) 585
XhoI CTCGAG 1 cut(s) 169
XspI CTAG 2 cut(s) 230, 645
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.