MD12G1136300.v1.1

Plant protein 1589 of unknown function (A_thal_3526)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009837
Physical Location & Seq
Reverse (-)
21280051 .. 21281314
1264 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1136300.v1.1.491

Sequence Viewer

Length: 318 bp
ATGGGAGACTCTTCTGCTTCATACATTCACTTGGTGCACCATTTAATCGAGGAGTGCATCATATTTTATATGAGCAAAGAGGAGTGCATGGAAGCCCTCTCTAAGCGTGCAAACATAAAACCCATCATTACCTCCACAGTGTGGAAGGAGTTAGAGAAGGAGAACAAGGAGTTCTTTGAGGCCTACAACAAGAGCAGGGAAGCTAGATCCTCTGAGATGGTCACAGAGCAAAGAAGCCAGAAGATGCTTTTTGATCTTTCTCAGAGGGGCGACAACTCCACCACCACCGATGACGGCGGCGATGAATCTGATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
A_thal_3526

Protein Analysis

106

Amino Acids

12.13

Weight (kDa)

4.83

Isoelectric Point (pI)

85.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
A_thal_3526 PF09713 12 - 63 2.6e-27 Plant protein 1589 of unknown function (A_thal_3526)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 141
AciI CCGC 1 cut(s) 297
AclWI GGATC 1 cut(s) 201
AdeI CACNNNGTG 2 cut(s) 34, 141
AfiI CCNNNNNNNGG 1 cut(s) 141
AluBI AGCT 1 cut(s) 203
AluI AGCT 1 cut(s) 203
Alw21I GWGCWC 1 cut(s) 39
Alw44I GTGCAC 1 cut(s) 35
AlwI GGATC 1 cut(s) 201
AoxI GGCC 1 cut(s) 180
ApaLI GTGCAC 1 cut(s) 35
BaeGI GKGCMC 1 cut(s) 39
Bbv12I GWGCWC 1 cut(s) 39
BccI CCATC 2 cut(s) 131, 211
BceAI ACGGC 1 cut(s) 310
BclI TGATCA 1 cut(s) 310
BfaI CTAG 1 cut(s) 204
BisI GCNGC 1 cut(s) 298
BlsI GCNGC 1 cut(s) 299
BmsI GCATC 2 cut(s) 66, 234
Bsc4I CCNNNNNNNGG 1 cut(s) 141
BseLI CCNNNNNNNGG 1 cut(s) 141
BseMII CTCAG 2 cut(s) 204, 275
BseRI GAGGAG 2 cut(s) 65, 95
BseSI GKGCMC 1 cut(s) 39
BshFI GGCC 1 cut(s) 182
BsiHKAI GWGCWC 1 cut(s) 39
BslI CCNNNNNNNGG 1 cut(s) 141
BsnI GGCC 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 3 cut(s) 206, 253, 310
BspACI CCGC 1 cut(s) 297
BspANI GGCC 1 cut(s) 182
BspCNI CTCAG 2 cut(s) 205, 274
BspPI GGATC 1 cut(s) 201
BssMI GATC 3 cut(s) 206, 253, 310
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 3 cut(s) 102, 213, 261
BstKTI GATC 3 cut(s) 209, 256, 313
BstMBI GATC 3 cut(s) 206, 253, 310
BstSLI GKGCMC 1 cut(s) 39
BstX2I RGATCY 1 cut(s) 206
BstYI RGATCY 1 cut(s) 206
BsuRI GGCC 1 cut(s) 182
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 108
CviAII CATG 1 cut(s) 88
CviJI RGCY 4 cut(s) 95, 182, 203, 237
CviKI_1 RGCY 4 cut(s) 95, 182, 203, 237
DdeI CTNAG 3 cut(s) 102, 213, 261
DpnI GATC 3 cut(s) 208, 255, 312
DpnII GATC 3 cut(s) 206, 253, 310
DraIII CACNNNGTG 2 cut(s) 34, 141
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Eco147I AGGCCT 1 cut(s) 182
FaeI CATG 1 cut(s) 91
FaiI YATR 6 cut(s) 22, 62, 69, 71, 89, 116
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FatI CATG 1 cut(s) 87
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 1 cut(s) 298
Fsp4HI GCNGC 1 cut(s) 298
FspBI CTAG 1 cut(s) 204
GluI GCNGC 1 cut(s) 298
HaeIII GGCC 1 cut(s) 182
Hin1II CATG 1 cut(s) 91
HinfI GANTC 2 cut(s) 8, 305
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 3 cut(s) 214, 264, 310
Hpy8I GTNNAC 1 cut(s) 37
HpyAV CCTTC 2 cut(s) 139, 151
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4V TGCA 4 cut(s) 37, 57, 87, 110
HpyF3I CTNAG 3 cut(s) 102, 213, 261
Hsp92II CATG 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 3 cut(s) 206, 253, 310
LpnPI CCDG 2 cut(s) 181, 251
LweI GCATC 2 cut(s) 66, 234
MaeI CTAG 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 220
MalI GATC 3 cut(s) 208, 255, 312
MboI GATC 3 cut(s) 206, 253, 310
MboII GAAGA 2 cut(s) 3, 253
MflI RGATCY 1 cut(s) 206
MhlI GDGCHC 1 cut(s) 39
MlyI GAGTC 1 cut(s) 2
MnlI CCTC 7 cut(s) 43, 73, 107, 142, 172, 220, 258
MseI TTAA 1 cut(s) 44
NdeII GATC 3 cut(s) 206, 253, 310
NlaIII CATG 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 220
PceI AGGCCT 1 cut(s) 182
PfeI GAWTC 1 cut(s) 305
PflMI CCANNNNNTGG 1 cut(s) 141
PkrI GCNGC 1 cut(s) 299
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
PsuI RGATCY 1 cut(s) 206
SaqAI TTAA 1 cut(s) 44
SatI GCNGC 1 cut(s) 298
Sau3AI GATC 3 cut(s) 206, 253, 310
SchI GAGTC 1 cut(s) 2
SduI GDGCHC 1 cut(s) 39
SetI ASST 2 cut(s) 134, 205
SfaNI GCATC 2 cut(s) 66, 234
SgeI CNNG 9 cut(s) 43, 61, 100, 119, 178, 202, 208, 216, 250
SseBI AGGCCT 1 cut(s) 182
SsiI CCGC 1 cut(s) 297
SspMI CTAG 1 cut(s) 204
StuI AGGCCT 1 cut(s) 182
TaaI ACNGT 1 cut(s) 139
TaqI TCGA 1 cut(s) 48
TauI GCSGC 1 cut(s) 300
TfiI GAWTC 1 cut(s) 305
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TscAI CASTG 1 cut(s) 144
TseFI GTSAC 1 cut(s) 220
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 2 cut(s) 9, 318
TspRI CASTG 1 cut(s) 144
Van91I CCANNNNNTGG 1 cut(s) 141
VneI GTGCAC 1 cut(s) 35
XspI CTAG 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.