MD04G1121200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010400
Physical Location & Seq
Forward (+)
20747290 .. 20750319
3030 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1121200.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGATTTGGTTAACTATGGACATAAGCTCTTCTAGTGGGAAATCGGTGTTGCGAAATGTAGACAGTTTACAAGGTTTGCAGCCTTCTGCGAACCAAGAGCAAATTCGTACTTTGCTTGAAGATTATTTAGTAAGCGACCTTGTAAGTGCTGTTGGTGAGTCCTTGAATATCAGAGGTATTGGAGAAGAGTCGATTGATGTTAAAGATGTTAGTGAAGAGTCTATTGATTCCAAAGATGTTGGAAAAGAGTCGATTGATGCCAAAGGTGTTGGAGAAGAGTCGATTGATGCCAAAGGTGTTGGAGAAGAGTCGATCGATGCCAAAGATGTTGGAGAAGTGTCCATCGATGCCAAAGATGTTGGAGAAGTGTCCGTCGATGCCAAAGATGTTGGAAAAGTGTCGATTGATGCCAAAGATGTTGGAAAAGAGTCGATTGAGATCAAAGGTGTTGGAGAAGTGGAACATGCACCTGATGGGGATAATACTAGTGGTGTGGAAAAAAGAAACACATATGAAGTTCCTAATGCACAACAGGGAAACGTCACTCGAAAATGCTTGAACAAGTGTGCAACCTTTCCTATTCCTACTGGTGTGCTCTCTCCTAATGCCTCAAGCATAGTAAAGGAGGAACCAGAAGAAGTGCTATGCGGGGATGAAAATCATCACCATGCCCACTCGCGCTCAGTGTCTTTGCCTTCTCCTTCGAAGCTACTTTCTGCCCTAAAAGGTAGCCGGGCGAAAGAGGGATTATCACCCGGGAAACTTTCTGTCAAATGGGCCCCTGATGTGTACGATCCGCCTGCTACATCAATGTCACATACAGTGACAGCCAAGAAACAGCAGAAGTCAAAGAACAAGAAAAATTGTAAGAAAGATGGAAAGAAATGGCAAAAGAGCAGTTCTTCACGAGGAAAAGATAAGAAGCAATATCGCAAATCTGGGTCTTCTGGGAGGTGTTACAGATCAATGGATCCCCGTGAAACATTGGTTGACACAAACAATGGTTTTGGTGAGCTTGTGGTAGGCAGCCCAGACCAGCATTCTTATTGTGGGAGCTCCTTCTTGAAAAATTCGCTCGCTAACATGCATTACCCGGTTGCTGAAGCCTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

371

Amino Acids

39.68

Weight (kDa)

5.74

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010400)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37480 AT2G37480 AT3G53670 AT3G53670
fragaria_vesca FvH4_6g14310
malus_domestica MD04G1121200.v1.1 MD12G1138400.v1.1
prunus_persica Prupe.6G250300_v2.0.a1
pyrus_communis pycom04g11040 pycom12g13240
rosa_chinensis RchiOBHm_Chr3g0467301
rosa_laevigata RLG00000024512
rosa_multiflora Rmu_sc0000962.1_g000068 Rmu_sc0012351.1_g000001
rosa_roxburghii Rroxscaffold_6G00413750
rosa_rugosa Rorug03G0086400
rosa_samantha Rh3AG134800 Rh3BG156100 Rh3CG155700 Rh3DG156900
rosa_wichuraiana Rw3G012760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 60
AccII CGCG 1 cut(s) 679
AciI CCGC 2 cut(s) 648, 797
AclWI GGATC 3 cut(s) 788, 965, 978
AcsI RAATTY 2 cut(s) 102, 1069
AfaI GTAC 2 cut(s) 109, 791
AgsI TTSAA 4 cut(s) 119, 166, 559, 1066
AhlI ACTAGT 1 cut(s) 485
AjuI GAANNNNNNNTTGG 2 cut(s) 87, 119
AluBI AGCT 4 cut(s) 27, 709, 1015, 1056
AluI AGCT 4 cut(s) 27, 709, 1015, 1056
Alw21I GWGCWC 2 cut(s) 597, 1058
AlwI GGATC 3 cut(s) 788, 965, 978
Ama87I CYCGRG 1 cut(s) 755
AoxI GGCC 1 cut(s) 777
ApaI GGGCCC 1 cut(s) 781
ApeKI GCWGC 2 cut(s) 79, 1026
ApoI RAATTY 2 cut(s) 102, 1069
AspLEI GCGC 1 cut(s) 681
AspS9I GGNCC 2 cut(s) 777, 778
AsuC2I CCSGG 4 cut(s) 734, 756, 757, 1094
AsuHPI GGTGA 4 cut(s) 167, 656, 744, 1022
AsuII TTCGAA 1 cut(s) 704
AvaI CYCGRG 1 cut(s) 755
BaeGI GKGCMC 1 cut(s) 781
BamHI GGATCC 1 cut(s) 970
BanII GRGCYC 2 cut(s) 781, 1058
BauI CACGAG 1 cut(s) 906
BbsI GAAGAC 1 cut(s) 936
Bbv12I GWGCWC 2 cut(s) 597, 1058
BbvI GCAGC 2 cut(s) 91, 1038
BccI CCATC 3 cut(s) 350, 467, 869
BcgI CGANNNNNNTGC 2 cut(s) 782, 816
BcnI CCSGG 4 cut(s) 734, 756, 757, 1094
BcuI ACTAGT 1 cut(s) 485
BfaI CTAG 2 cut(s) 33, 486
BisI GCNGC 2 cut(s) 80, 1027
BlsI GCNGC 2 cut(s) 81, 1028
Bme1390I CCNGG 4 cut(s) 734, 756, 757, 1094
BmeT110I CYCGRG 1 cut(s) 755
BmgT120I GGNCC 2 cut(s) 777, 778
BmiI GGNNCC 4 cut(s) 630, 779, 780, 972
BmrFI CCNGG 4 cut(s) 734, 756, 757, 1094
BmsI GCATC 6 cut(s) 247, 277, 307, 337, 367, 397
BpiI GAAGAC 1 cut(s) 936
Bpu14I TTCGAA 1 cut(s) 704
BpuEI CTTGAG 1 cut(s) 595
BpuMI CCSGG 4 cut(s) 734, 756, 757, 1094
Bsa29I ATCGAT 2 cut(s) 315, 345
BsaBI GATNNNNATC 2 cut(s) 437, 657
BsaJI CCNNGG 1 cut(s) 755
Bse1I ACTGG 1 cut(s) 592
Bse8I GATNNNNATC 2 cut(s) 437, 657
BseCI ATCGAT 2 cut(s) 315, 345
BseDI CCNNGG 1 cut(s) 755
BseGI GGATG 1 cut(s) 658
BseJI GATNNNNATC 2 cut(s) 437, 657
BseMII CTCAG 1 cut(s) 696
BseNI ACTGG 1 cut(s) 592
BseSI GKGCMC 1 cut(s) 781
BseXI GCAGC 2 cut(s) 91, 1038
Bsh1236I CGCG 1 cut(s) 679
Bsh1285I CGRYCG 1 cut(s) 315
BshFI GGCC 1 cut(s) 779
BshVI ATCGAT 2 cut(s) 315, 345
BsiEI CGRYCG 1 cut(s) 315
BsiHKAI GWGCWC 2 cut(s) 597, 1058
BsiHKCI CYCGRG 1 cut(s) 755
BsiSI CCGG 3 cut(s) 733, 756, 1094
BsmI GAATGC 1 cut(s) 1039
BsnI GGCC 1 cut(s) 779
BsoBI CYCGRG 1 cut(s) 755
Bsp119I TTCGAA 1 cut(s) 704
Bsp120I GGGCCC 1 cut(s) 777
Bsp1286I GDGCHC 3 cut(s) 597, 781, 1058
Bsp143I GATC 5 cut(s) 312, 438, 793, 962, 970
BspACI CCGC 2 cut(s) 648, 797
BspANI GGCC 1 cut(s) 779
BspCNI CTCAG 1 cut(s) 695
BspDI ATCGAT 2 cut(s) 315, 345
BspFNI CGCG 1 cut(s) 679
BspLI GGNNCC 4 cut(s) 630, 779, 780, 972
BspPI GGATC 3 cut(s) 788, 965, 978
BspQI GCTCTTC 1 cut(s) 34
BspT104I TTCGAA 1 cut(s) 704
BsrI ACTGG 1 cut(s) 592
BssECI CCNNGG 1 cut(s) 755
BssMI GATC 5 cut(s) 312, 438, 793, 962, 970
BssSI CACGAG 1 cut(s) 906
Bst2BI CACGAG 1 cut(s) 906
Bst4CI ACNGT 2 cut(s) 65, 823
Bst6I CTCTTC 5 cut(s) 34, 180, 210, 270, 300
BstBI TTCGAA 1 cut(s) 704
BstC8I GCNNGC 2 cut(s) 801, 1077
BstDEI CTNAG 1 cut(s) 682
BstF5I GGATG 1 cut(s) 658
BstFNI CGCG 1 cut(s) 679
BstHHI GCGC 1 cut(s) 681
BstKTI GATC 5 cut(s) 315, 441, 796, 965, 973
BstMBI GATC 5 cut(s) 312, 438, 793, 962, 970
BstMCI CGRYCG 1 cut(s) 315
BstNSI RCATGY 2 cut(s) 467, 1087
BstSCI CCNGG 4 cut(s) 732, 754, 755, 1092
BstSLI GKGCMC 1 cut(s) 781
BstUI CGCG 1 cut(s) 679
BstV1I GCAGC 2 cut(s) 91, 1038
BstV2I GAAGAC 1 cut(s) 936
BstX2I RGATCY 1 cut(s) 970
BstYI RGATCY 1 cut(s) 970
Bsu15I ATCGAT 2 cut(s) 315, 345
BsuRI GGCC 1 cut(s) 779
BsuTUI ATCGAT 2 cut(s) 315, 345
BtsCI GGATG 1 cut(s) 658
BtsIMutI CAGTG 2 cut(s) 690, 828
Cac8I GCNNGC 2 cut(s) 801, 1077
CfoI GCGC 1 cut(s) 681
Cfr13I GGNCC 2 cut(s) 777, 778
Cfr9I CCCGGG 1 cut(s) 755
ClaI ATCGAT 2 cut(s) 315, 345
Csp6I GTAC 2 cut(s) 108, 790
CviAII CATG 3 cut(s) 464, 668, 1084
CviQI GTAC 2 cut(s) 108, 790
DdeI CTNAG 1 cut(s) 682
DpnI GATC 5 cut(s) 314, 440, 795, 964, 972
DpnII GATC 5 cut(s) 312, 438, 793, 962, 970
Eam1104I CTCTTC 5 cut(s) 34, 180, 210, 270, 300
EarI CTCTTC 5 cut(s) 34, 180, 210, 270, 300
EciI GGCGGA 1 cut(s) 786
Ecl136II GAGCTC 1 cut(s) 1056
Eco24I GRGCYC 2 cut(s) 781, 1058
Eco53kI GAGCTC 1 cut(s) 1056
Eco88I CYCGRG 1 cut(s) 755
EcoICRI GAGCTC 1 cut(s) 1056
EcoO109I RGGNCCY 1 cut(s) 778
EcoT22I ATGCAT 1 cut(s) 1089
EcoT38I GRGCYC 2 cut(s) 781, 1058
FaeI CATG 3 cut(s) 467, 671, 1087
FatI CATG 3 cut(s) 463, 667, 1083
FauI CCCGC 1 cut(s) 641
FauNDI CATATG 1 cut(s) 511
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 2 cut(s) 80, 1027
FokI GGATG 1 cut(s) 665
FriOI GRGCYC 2 cut(s) 781, 1058
Fsp4HI GCNGC 2 cut(s) 80, 1027
FspBI CTAG 2 cut(s) 33, 486
GlaI GCGC 1 cut(s) 680
GluI GCNGC 2 cut(s) 80, 1027
HaeIII GGCC 1 cut(s) 779
HapII CCGG 3 cut(s) 733, 756, 1094
HhaI GCGC 1 cut(s) 681
Hin1II CATG 3 cut(s) 467, 671, 1087
Hin6I GCGC 1 cut(s) 679
HinP1I GCGC 1 cut(s) 679
HincII GTYRAC 2 cut(s) 12, 991
HindII GTYRAC 2 cut(s) 12, 991
HinfI GANTC 8 cut(s) 158, 188, 218, 227, 248, 278, 308, 428
HpaI GTTAAC 1 cut(s) 12
HpaII CCGG 3 cut(s) 733, 756, 1094
HphI GGTGA 4 cut(s) 167, 656, 744, 1022
Hpy166II GTNNAC 5 cut(s) 12, 61, 68, 790, 991
Hpy188I TCNGA 1 cut(s) 173
Hpy188III TCNNGA 2 cut(s) 906, 1063
Hpy8I GTNNAC 5 cut(s) 12, 61, 68, 790, 991
Hpy99I CGWCG 1 cut(s) 377
HpyAV CCTTC 4 cut(s) 93, 705, 711, 1069
HpyCH4III ACNGT 2 cut(s) 65, 823
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 5 cut(s) 79, 467, 527, 569, 1087
HpyF3I CTNAG 1 cut(s) 682
HpySE526I ACGT 1 cut(s) 540
Hsp92II CATG 3 cut(s) 467, 671, 1087
HspAI GCGC 1 cut(s) 679
KspAI GTTAAC 1 cut(s) 12
Kzo9I GATC 5 cut(s) 312, 438, 793, 962, 970
LguI GCTCTTC 1 cut(s) 34
LmnI GCTCC 2 cut(s) 1053, 1061
Lsp1109I GCAGC 2 cut(s) 91, 1038
LweI GCATC 6 cut(s) 247, 277, 307, 337, 367, 397
MaeI CTAG 2 cut(s) 33, 486
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 4 cut(s) 541, 813, 823, 956
MalI GATC 5 cut(s) 314, 440, 795, 964, 972
MboI GATC 5 cut(s) 312, 438, 793, 962, 970
MboII GAAGA 9 cut(s) 21, 131, 197, 227, 287, 317, 647, 894, 936
MflI RGATCY 1 cut(s) 970
MhlI GDGCHC 3 cut(s) 597, 781, 1058
MluCI AATT 3 cut(s) 102, 862, 1069
MlyI GAGTC 7 cut(s) 167, 197, 227, 257, 287, 317, 437
MmeI TCCRAC 8 cut(s) 220, 250, 280, 310, 340, 370, 400, 430
MnlI CCTC 6 cut(s) 167, 619, 619, 736, 902, 945
Mph1103I ATGCAT 1 cut(s) 1089
MseI TTAA 2 cut(s) 11, 201
MslI CAYNNNNRTG 1 cut(s) 666
MspI CCGG 3 cut(s) 733, 756, 1094
MspR9I CCNGG 4 cut(s) 734, 756, 757, 1094
Mva1269I GAATGC 1 cut(s) 1039
MvnI CGCG 1 cut(s) 679
NciI CCSGG 4 cut(s) 734, 756, 757, 1094
NdeI CATATG 1 cut(s) 511
NdeII GATC 5 cut(s) 312, 438, 793, 962, 970
NlaIII CATG 3 cut(s) 467, 671, 1087
NlaIV GGNNCC 4 cut(s) 630, 779, 780, 972
NmuCI GTSAC 3 cut(s) 541, 813, 823
NsiI ATGCAT 1 cut(s) 1089
NspI RCATGY 2 cut(s) 467, 1087
NspV TTCGAA 1 cut(s) 704
PciSI GCTCTTC 1 cut(s) 34
PctI GAATGC 1 cut(s) 1039
PfeI GAWTC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 81, 1028
Ple19I CGATCG 1 cut(s) 315
PleI GAGTC 7 cut(s) 166, 196, 226, 256, 286, 316, 436
PpsI GAGTC 7 cut(s) 166, 196, 226, 256, 286, 316, 436
Psp124BI GAGCTC 1 cut(s) 1058
PspN4I GGNNCC 4 cut(s) 630, 779, 780, 972
PspOMI GGGCCC 1 cut(s) 777
PspPI GGNCC 2 cut(s) 777, 778
PsuI RGATCY 1 cut(s) 970
PvuI CGATCG 1 cut(s) 315
RsaI GTAC 2 cut(s) 109, 791
RsaNI GTAC 2 cut(s) 108, 790
RseI CAYNNNNRTG 1 cut(s) 666
SacI GAGCTC 1 cut(s) 1058
SapI GCTCTTC 1 cut(s) 34
SaqAI TTAA 2 cut(s) 11, 201
SatI GCNGC 2 cut(s) 80, 1027
Sau3AI GATC 5 cut(s) 312, 438, 793, 962, 970
Sau96I GGNCC 2 cut(s) 777, 778
SchI GAGTC 7 cut(s) 167, 197, 227, 257, 287, 317, 437
ScrFI CCNGG 4 cut(s) 734, 756, 757, 1094
SduI GDGCHC 3 cut(s) 597, 781, 1058
SfaNI GCATC 6 cut(s) 247, 277, 307, 337, 367, 397
SfuI TTCGAA 1 cut(s) 704
SmaI CCCGGG 1 cut(s) 757
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 1 cut(s) 610
SmoI CTYRAG 1 cut(s) 610
SpeI ACTAGT 1 cut(s) 485
Sse9I AATT 3 cut(s) 102, 862, 1069
SsiI CCGC 2 cut(s) 648, 797
SspMI CTAG 2 cut(s) 33, 486
SstI GAGCTC 1 cut(s) 1058
StyD4I CCNGG 4 cut(s) 732, 754, 755, 1092
TaaI ACNGT 2 cut(s) 65, 823
TaiI ACGT 1 cut(s) 543
TasI AATT 3 cut(s) 102, 862, 1069
TfiI GAWTC 1 cut(s) 227
Tru1I TTAA 2 cut(s) 11, 201
Tru9I TTAA 2 cut(s) 11, 201
TscAI CASTG 2 cut(s) 690, 828
TseFI GTSAC 3 cut(s) 541, 813, 823
TseI GCWGC 2 cut(s) 79, 1026
Tsp45I GTSAC 3 cut(s) 541, 813, 823
TspDTI ATGAA 2 cut(s) 528, 669
TspGWI ACGGA 1 cut(s) 361
TspMI CCCGGG 1 cut(s) 755
TspRI CASTG 2 cut(s) 690, 828
XapI RAATTY 2 cut(s) 102, 1069
XceI RCATGY 2 cut(s) 467, 1087
XmaI CCCGGG 1 cut(s) 755
XmiI GTMKAC 1 cut(s) 60
XspI CTAG 2 cut(s) 33, 486
Zsp2I ATGCAT 1 cut(s) 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.