pycom12g13240

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010400
Physical Location & Seq
Forward (+)
15702046 .. 15703733
1688 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g13240.3

Sequence Viewer

Length: 954 bp
ATGGACATAAGCTTTCCTCGTGGGAAATCGGTGTTGCGAAATGTAGACAGTTTGCAAGGTTTGCAGCCCTCTGTGAACCAAGAGCAAATTCGTACTAGTTTGCTTGAAGATTATTCGGTAAGCGGTCTTGTAAGTGCTGTTGGCGAGTCCTTGAATATCAACGTTGGAGAAGACTCGATTGATGTTAAAGATGTTGGAAAAGAGTCGACTGATGTCAAAGATGTTGGAAAAGAGTCGACTGATGTCAAAGATGTTGGAAAAGAACATTCGACTGATGTCAAACATGTTGGAGAAGTGGAACTTGCTTCTGATGGGTATAATACTGTCGGTGTGGAGAAGAGAAACACACATGAAGATCCTAAAGCACAGCAGGGAAAAGTCACTCGAAAATGCTTGAACAAGTGTGCAACCTTTCCTAATCCTACTGGTGTGCTCTCTCCTAATGCCTCCGCAAGCATAGTAAAGGAGGAACCTAAAGAAGCACTATGCGGGGATGAACATCATCACCAAGCCTGCTTGCGCTCGGTGTCTTTGCCTGCTCCTTCGAAGCTACTTTCTGCTCTGAAAGGTAGCCGGGAGAAAGAGGGCTTATCACCCGGGAAATTTTCTGTCACTTGGGCTCCTGATGTGTACGATCCGCCTGCTACATCAATGTCGCACACAGTTACAGGCAAGAAACAGCAGAAGTCAAAGAACAAGAAGAATTGGAAGAAAGATGGAAAGAAGGGGCAAAAGAGCAGCTCTTCACGAGGAAAAGATAAGAAGCAATATCGCAAATCTGGGGCTTCTGAGAGGTGTCGCAGATCTCTGGATCCCCGTGAAACATTGGTCGACACAAACAATGGTTTTGGTGAGCTTGTGGTGGGCAGCCCAGACCAGCATTCTTATTGTGGGAGCTCCTTCTTGAAAAATTCGCTCACTAACGTGCATTACCCAGTTGCAGAAGCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

318

Amino Acids

34.37

Weight (kDa)

8.83

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010400)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37480 AT2G37480 AT3G53670 AT3G53670
fragaria_vesca FvH4_6g14310
malus_domestica MD04G1121200.v1.1 MD12G1138400.v1.1
prunus_persica Prupe.6G250300_v2.0.a1
pyrus_communis pycom04g11040 pycom12g13240
rosa_chinensis RchiOBHm_Chr3g0467301
rosa_laevigata RLG00000024512
rosa_multiflora Rmu_sc0000962.1_g000068 Rmu_sc0012351.1_g000001
rosa_roxburghii Rroxscaffold_6G00413750
rosa_rugosa Rorug03G0086400
rosa_samantha Rh3AG134800 Rh3BG156100 Rh3CG155700 Rh3DG156900
rosa_wichuraiana Rw3G012760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 4 cut(s) 45, 206, 236, 831
AciI CCGC 4 cut(s) 123, 450, 489, 638
AclI AACGTT 1 cut(s) 162
AclWI GGATC 4 cut(s) 350, 629, 806, 819
AcsI RAATTY 3 cut(s) 87, 602, 910
AfaI GTAC 2 cut(s) 94, 632
AflIII ACRYGT 1 cut(s) 283
AgsI TTSAA 4 cut(s) 107, 154, 397, 907
AhlI ACTAGT 1 cut(s) 95
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
AleI CACNNNNGTG 1 cut(s) 923
AluBI AGCT 5 cut(s) 12, 550, 741, 856, 897
AluI AGCT 5 cut(s) 12, 550, 741, 856, 897
Alw21I GWGCWC 2 cut(s) 435, 899
AlwI GGATC 4 cut(s) 350, 629, 806, 819
Ama87I CYCGRG 1 cut(s) 596
ApeKI GCWGC 3 cut(s) 64, 738, 867
ApoI RAATTY 3 cut(s) 87, 602, 910
AspLEI GCGC 1 cut(s) 522
AsuC2I CCSGG 3 cut(s) 575, 597, 598
AsuHPI GGTGA 3 cut(s) 497, 585, 863
AsuII TTCGAA 1 cut(s) 545
AvaI CYCGRG 1 cut(s) 596
BamHI GGATCC 1 cut(s) 811
BanII GRGCYC 2 cut(s) 622, 899
BauI CACGAG 2 cut(s) 18, 747
BbsI GAAGAC 1 cut(s) 177
Bbv12I GWGCWC 2 cut(s) 435, 899
BbvI GCAGC 3 cut(s) 76, 750, 879
BccI CCATC 2 cut(s) 305, 710
BcgI CGANNNNNNTGC 2 cut(s) 623, 657
BcnI CCSGG 3 cut(s) 575, 597, 598
BcuI ACTAGT 1 cut(s) 95
BfaI CTAG 1 cut(s) 96
BglII AGATCT 1 cut(s) 803
BisI GCNGC 3 cut(s) 65, 739, 868
BlsI GCNGC 3 cut(s) 66, 740, 869
Bme1390I CCNGG 3 cut(s) 575, 597, 598
BmeT110I CYCGRG 1 cut(s) 596
BmiI GGNNCC 3 cut(s) 471, 621, 813
BmrFI CCNGG 3 cut(s) 575, 597, 598
BmrI ACTGGG 1 cut(s) 929
BmuI ACTGGG 1 cut(s) 929
BoxI GACNNNNGTC 3 cut(s) 212, 242, 275
BpiI GAAGAC 1 cut(s) 177
Bpu14I TTCGAA 1 cut(s) 545
BpuMI CCSGG 3 cut(s) 575, 597, 598
BsaBI GATNNNNATC 1 cut(s) 498
BsaJI CCNNGG 1 cut(s) 596
BsaXI ACNNNNNCTCC 2 cut(s) 604, 634
Bse1I ACTGG 2 cut(s) 430, 935
Bse8I GATNNNNATC 1 cut(s) 498
BseDI CCNNGG 1 cut(s) 596
BseGI GGATG 1 cut(s) 499
BseJI GATNNNNATC 1 cut(s) 498
BseMII CTCAG 1 cut(s) 780
BseNI ACTGG 2 cut(s) 430, 935
BseXI GCAGC 3 cut(s) 76, 750, 879
BsiHKAI GWGCWC 2 cut(s) 435, 899
BsiHKCI CYCGRG 1 cut(s) 596
BsiSI CCGG 2 cut(s) 574, 597
BsmI GAATGC 1 cut(s) 880
BsoBI CYCGRG 1 cut(s) 596
Bsp119I TTCGAA 1 cut(s) 545
Bsp1286I GDGCHC 3 cut(s) 435, 622, 899
Bsp143I GATC 4 cut(s) 355, 634, 803, 811
BspACI CCGC 4 cut(s) 123, 450, 489, 638
BspCNI CTCAG 1 cut(s) 781
BspLI GGNNCC 3 cut(s) 471, 621, 813
BspPI GGATC 4 cut(s) 350, 629, 806, 819
BspQI GCTCTTC 1 cut(s) 748
BspT104I TTCGAA 1 cut(s) 545
BsrI ACTGG 2 cut(s) 430, 935
BssECI CCNNGG 1 cut(s) 596
BssMI GATC 4 cut(s) 355, 634, 803, 811
BssSI CACGAG 2 cut(s) 18, 747
Bst2BI CACGAG 2 cut(s) 18, 747
Bst4CI ACNGT 3 cut(s) 50, 325, 664
Bst6I CTCTTC 2 cut(s) 332, 748
BstAPI GCANNNNNTGC 1 cut(s) 61
BstBI TTCGAA 1 cut(s) 545
BstC8I GCNNGC 5 cut(s) 454, 514, 518, 537, 642
BstDEI CTNAG 1 cut(s) 789
BstF5I GGATG 1 cut(s) 499
BstHHI GCGC 1 cut(s) 522
BstKTI GATC 4 cut(s) 358, 637, 806, 814
BstMBI GATC 4 cut(s) 355, 634, 803, 811
BstMWI GCNNNNNNNGC 1 cut(s) 61
BstNSI RCATGY 1 cut(s) 287
BstPAI GACNNNNGTC 3 cut(s) 212, 242, 275
BstSCI CCNGG 3 cut(s) 573, 595, 596
BstV1I GCAGC 3 cut(s) 76, 750, 879
BstV2I GAAGAC 1 cut(s) 177
BstX2I RGATCY 3 cut(s) 355, 803, 811
BstYI RGATCY 3 cut(s) 355, 803, 811
BtsCI GGATG 1 cut(s) 499
Cac8I GCNNGC 5 cut(s) 454, 514, 518, 537, 642
CfoI GCGC 1 cut(s) 522
Cfr9I CCCGGG 1 cut(s) 596
Csp6I GTAC 2 cut(s) 93, 631
CviAII CATG 2 cut(s) 284, 350
CviQI GTAC 2 cut(s) 93, 631
DdeI CTNAG 1 cut(s) 789
DpnI GATC 4 cut(s) 357, 636, 805, 813
DpnII GATC 4 cut(s) 355, 634, 803, 811
Eam1104I CTCTTC 2 cut(s) 332, 748
EarI CTCTTC 2 cut(s) 332, 748
EciI GGCGGA 1 cut(s) 627
Ecl136II GAGCTC 1 cut(s) 897
Eco24I GRGCYC 2 cut(s) 622, 899
Eco53kI GAGCTC 1 cut(s) 897
Eco88I CYCGRG 1 cut(s) 596
EcoICRI GAGCTC 1 cut(s) 897
EcoT38I GRGCYC 2 cut(s) 622, 899
FaeI CATG 2 cut(s) 287, 353
FaiI YATR 6 cut(s) 8, 285, 318, 351, 458, 487
FalI AAGNNNNNCTT 2 cut(s) 285, 317
FatI CATG 2 cut(s) 283, 349
FauI CCCGC 1 cut(s) 482
FblI GTMKAC 4 cut(s) 45, 206, 236, 831
Fnu4HI GCNGC 3 cut(s) 65, 739, 868
FokI GGATG 1 cut(s) 506
FriOI GRGCYC 2 cut(s) 622, 899
Fsp4HI GCNGC 3 cut(s) 65, 739, 868
FspBI CTAG 1 cut(s) 96
GlaI GCGC 1 cut(s) 521
GluI GCNGC 3 cut(s) 65, 739, 868
HapII CCGG 2 cut(s) 574, 597
HhaI GCGC 1 cut(s) 522
Hin1II CATG 2 cut(s) 287, 353
Hin6I GCGC 1 cut(s) 520
HinP1I GCGC 1 cut(s) 520
HincII GTYRAC 3 cut(s) 207, 237, 832
HindII GTYRAC 3 cut(s) 207, 237, 832
HindIII AAGCTT 1 cut(s) 10
HinfI GANTC 4 cut(s) 146, 173, 203, 233
HpaII CCGG 2 cut(s) 574, 597
HphI GGTGA 3 cut(s) 497, 585, 863
Hpy166II GTNNAC 6 cut(s) 46, 76, 207, 237, 631, 832
Hpy188I TCNGA 3 cut(s) 310, 564, 790
Hpy188III TCNNGA 4 cut(s) 623, 747, 809, 904
Hpy8I GTNNAC 6 cut(s) 46, 76, 207, 237, 631, 832
HpyAV CCTTC 3 cut(s) 552, 718, 910
HpyCH4III ACNGT 3 cut(s) 50, 325, 664
HpyCH4IV ACGT 2 cut(s) 162, 924
HpyCH4V TGCA 5 cut(s) 55, 64, 407, 928, 941
HpyF10VI GCNNNNNNNGC 1 cut(s) 61
HpyF3I CTNAG 1 cut(s) 789
HpySE526I ACGT 2 cut(s) 162, 924
Hsp92II CATG 2 cut(s) 287, 353
HspAI GCGC 1 cut(s) 520
Kzo9I GATC 4 cut(s) 355, 634, 803, 811
LguI GCTCTTC 1 cut(s) 748
LmnI GCTCC 4 cut(s) 544, 625, 894, 902
Lsp1109I GCAGC 3 cut(s) 76, 750, 879
MaeI CTAG 1 cut(s) 96
MaeII ACGT 2 cut(s) 162, 924
MaeIII GTNAC 3 cut(s) 379, 610, 664
MalI GATC 4 cut(s) 357, 636, 805, 813
MboI GATC 4 cut(s) 355, 634, 803, 811
MboII GAAGA 7 cut(s) 119, 182, 349, 365, 712, 721, 735
MflI RGATCY 3 cut(s) 355, 803, 811
MhlI GDGCHC 3 cut(s) 435, 622, 899
MluCI AATT 4 cut(s) 87, 602, 703, 910
MlyI GAGTC 4 cut(s) 155, 167, 212, 242
MmeI TCCRAC 5 cut(s) 145, 175, 205, 235, 268
MnlI CCTC 7 cut(s) 27, 79, 457, 460, 577, 743, 786
MseI TTAA 1 cut(s) 186
MslI CAYNNNNRTG 1 cut(s) 923
MspI CCGG 2 cut(s) 574, 597
MspR9I CCNGG 3 cut(s) 575, 597, 598
Mva1269I GAATGC 1 cut(s) 880
MwoI GCNNNNNNNGC 1 cut(s) 61
NciI CCSGG 3 cut(s) 575, 597, 598
NdeII GATC 4 cut(s) 355, 634, 803, 811
NlaIII CATG 2 cut(s) 287, 353
NlaIV GGNNCC 3 cut(s) 471, 621, 813
NmuCI GTSAC 2 cut(s) 379, 610
NspI RCATGY 1 cut(s) 287
NspV TTCGAA 1 cut(s) 545
OliI CACNNNNGTG 1 cut(s) 923
PciI ACATGT 1 cut(s) 283
PciSI GCTCTTC 1 cut(s) 748
PctI GAATGC 1 cut(s) 880
PkrI GCNGC 3 cut(s) 66, 740, 869
PleI GAGTC 4 cut(s) 154, 167, 211, 241
PpsI GAGTC 4 cut(s) 154, 167, 211, 241
PscI ACATGT 1 cut(s) 283
PshAI GACNNNNGTC 3 cut(s) 212, 242, 275
Psp124BI GAGCTC 1 cut(s) 899
Psp1406I AACGTT 1 cut(s) 162
PspN4I GGNNCC 3 cut(s) 471, 621, 813
PsuI RGATCY 3 cut(s) 355, 803, 811
RsaI GTAC 2 cut(s) 94, 632
RsaNI GTAC 2 cut(s) 93, 631
RseI CAYNNNNRTG 1 cut(s) 923
SacI GAGCTC 1 cut(s) 899
SalI GTCGAC 3 cut(s) 205, 235, 830
SapI GCTCTTC 1 cut(s) 748
SaqAI TTAA 1 cut(s) 186
SatI GCNGC 3 cut(s) 65, 739, 868
Sau3AI GATC 4 cut(s) 355, 634, 803, 811
SchI GAGTC 4 cut(s) 155, 167, 212, 242
ScrFI CCNGG 3 cut(s) 575, 597, 598
SduI GDGCHC 3 cut(s) 435, 622, 899
SfuI TTCGAA 1 cut(s) 545
SmaI CCCGGG 1 cut(s) 598
SmiMI CAYNNNNRTG 1 cut(s) 923
SpeI ACTAGT 1 cut(s) 95
Sse9I AATT 4 cut(s) 87, 602, 703, 910
SsiI CCGC 4 cut(s) 123, 450, 489, 638
SspMI CTAG 1 cut(s) 96
SstI GAGCTC 1 cut(s) 899
StyD4I CCNGG 3 cut(s) 573, 595, 596
TaaI ACNGT 3 cut(s) 50, 325, 664
TaiI ACGT 2 cut(s) 165, 927
TaqI TCGA 7 cut(s) 176, 206, 236, 269, 385, 545, 831
TasI AATT 4 cut(s) 87, 602, 703, 910
Tru1I TTAA 1 cut(s) 186
Tru9I TTAA 1 cut(s) 186
TseFI GTSAC 2 cut(s) 379, 610
TseI GCWGC 3 cut(s) 64, 738, 867
Tsp45I GTSAC 2 cut(s) 379, 610
TspDTI ATGAA 2 cut(s) 366, 510
TspMI CCCGGG 1 cut(s) 596
XapI RAATTY 3 cut(s) 87, 602, 910
XceI RCATGY 1 cut(s) 287
XmaI CCCGGG 1 cut(s) 596
XmiI GTMKAC 4 cut(s) 45, 206, 236, 831
XspI CTAG 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.