MD12G1138400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010400
Physical Location & Seq
Forward (+)
21586673 .. 21589528
2856 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1138400.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGATGTTTGATATGGACATAAGCTCTCCTCGTGGGAAATCGGTGTTGCGAAATGTAGACAGTTTGCAAGGTTTGCAGCCCTCTGTGAACCAAGAGCAAATTCGTACTAGTTTGCTTGAAGATTATTCGGTAAGCGGTCTTGTAAGTGCTGTCGGTGAGTCCTTGAATATCAGCGTTGGAGAAGTCTCGACTGATGTTAAAGATGTTGGAAGAGGGTCGACTGATATCAAAGATGTTGGAAGAGAGTCGACTGATGTCAAAGATGTTGGAAAAGAGTCGAGGATTGATGTTAAAGATGTTGGAAGAGGGTCAATTGATGTCAAACATGTTGGAAAAGAGTCGATTGATGTCAAACATGTTGGAGAAGTGGAACATGCTTCTGATGGGTATAATACTATTGGTGTGGAGAAGAGAAACACACATGAAGATCCTAATGCACAGCAGGGAAAAGTCACTCGAAAATGCTTGAACAAGTGTGCAATCTTTCCTAATCCTACTGGCGTGCTCTCTCCTAATGCCTCCGCAAGCATAGTAAAGGAGGAACCTAAAGAAGCACTATGCGGGGATGAACATCATCACCAAGCCTGCTTGTGCTCGGTGTCTTTGCCTGCTCCTACGAAGCTACTTTCTGCTCTGAAAGGTAGCCGGGAGAAAGAGGGCTTATCACCCGGGAAACTTTCTGTCACTTGGGCCCCTGATGTGTACGATCCGCCTGCTACATCAATGTCGCACACAGTTACAGGCAAGAAACAGCAGAAGTCAAAGAACAAGAAGAATTGGAAGAAAGATGGAAAGAAGGGGCAAAAGAGCAGCTCATCACGAGGAAAAGATAAGAAGCAATATCGCAAATCTGGGGCTTCTGAGAGGTGTCACAGATCTCTGGATCCCCGTGAAACATTGGTCGACCCAAACAATGGTTTTGGTGAGCTTGTGGTAGGCAGCCCAGACCAGCATTCTTATTGTGGGAGCTCCTTCTTGAAAAATTCGCTTACTAACGTGCATTACCCAGTTGCTGAAGCCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

342

Amino Acids

36.87

Weight (kDa)

8.67

Isoelectric Point (pI)

33.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010400)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37480 AT2G37480 AT3G53670 AT3G53670
fragaria_vesca FvH4_6g14310
malus_domestica MD04G1121200.v1.1 MD12G1138400.v1.1
prunus_persica Prupe.6G250300_v2.0.a1
pyrus_communis pycom04g11040 pycom12g13240
rosa_chinensis RchiOBHm_Chr3g0467301
rosa_laevigata RLG00000024512
rosa_multiflora Rmu_sc0000962.1_g000068 Rmu_sc0012351.1_g000001
rosa_roxburghii Rroxscaffold_6G00413750
rosa_rugosa Rorug03G0086400
rosa_samantha Rh3AG134800 Rh3BG156100 Rh3CG155700 Rh3DG156900
rosa_wichuraiana Rw3G012760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 914
AccI GTMKAC 4 cut(s) 57, 218, 248, 903
AciI CCGC 4 cut(s) 135, 522, 561, 710
AclWI GGATC 4 cut(s) 422, 701, 878, 891
AcsI RAATTY 2 cut(s) 99, 982
AfaI GTAC 2 cut(s) 106, 704
AfiI CCNNNNNNNGG 1 cut(s) 914
AflIII ACRYGT 2 cut(s) 325, 355
AgsI TTSAA 4 cut(s) 119, 166, 469, 979
AhlI ACTAGT 1 cut(s) 107
AjuI GAANNNNNNNTTGG 2 cut(s) 84, 116
AluBI AGCT 5 cut(s) 24, 622, 813, 928, 969
AluI AGCT 5 cut(s) 24, 622, 813, 928, 969
Alw21I GWGCWC 3 cut(s) 507, 596, 971
Alw26I GTCTC 1 cut(s) 190
AlwI GGATC 4 cut(s) 422, 701, 878, 891
AlwNI CAGNNNCTG 1 cut(s) 1013
Ama87I CYCGRG 1 cut(s) 668
AoxI GGCC 1 cut(s) 690
ApaI GGGCCC 1 cut(s) 694
ApeKI GCWGC 3 cut(s) 76, 810, 939
ApoI RAATTY 2 cut(s) 99, 982
AspS9I GGNCC 2 cut(s) 690, 691
AsuC2I CCSGG 3 cut(s) 647, 669, 670
AsuHPI GGTGA 4 cut(s) 167, 569, 657, 935
AvaI CYCGRG 1 cut(s) 668
BaeGI GKGCMC 1 cut(s) 694
BamHI GGATCC 1 cut(s) 883
BanII GRGCYC 2 cut(s) 694, 971
BauI CACGAG 2 cut(s) 30, 819
Bbv12I GWGCWC 3 cut(s) 507, 596, 971
BbvI GCAGC 3 cut(s) 88, 822, 951
BccI CCATC 2 cut(s) 377, 782
BcgI CGANNNNNNTGC 2 cut(s) 695, 729
BcnI CCSGG 3 cut(s) 647, 669, 670
BcoDI GTCTC 1 cut(s) 190
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 1 cut(s) 108
BglII AGATCT 1 cut(s) 875
BisI GCNGC 3 cut(s) 77, 811, 940
BlsI GCNGC 3 cut(s) 78, 812, 941
Bme1390I CCNGG 3 cut(s) 647, 669, 670
BmeT110I CYCGRG 1 cut(s) 668
BmgT120I GGNCC 2 cut(s) 690, 691
BmiI GGNNCC 4 cut(s) 543, 692, 693, 885
BmrFI CCNGG 3 cut(s) 647, 669, 670
BmrI ACTGGG 1 cut(s) 1001
BmuI ACTGGG 1 cut(s) 1001
BoxI GACNNNNGTC 1 cut(s) 254
BpuMI CCSGG 3 cut(s) 647, 669, 670
BsaBI GATNNNNATC 1 cut(s) 570
BsaJI CCNNGG 1 cut(s) 668
Bsc4I CCNNNNNNNGG 1 cut(s) 914
Bse1I ACTGG 2 cut(s) 502, 1007
Bse8I GATNNNNATC 1 cut(s) 570
BseDI CCNNGG 1 cut(s) 668
BseGI GGATG 1 cut(s) 571
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 1 cut(s) 914
BseMII CTCAG 1 cut(s) 852
BseNI ACTGG 2 cut(s) 502, 1007
BseRI GAGGAG 1 cut(s) 18
BseSI GKGCMC 1 cut(s) 694
BseXI GCAGC 3 cut(s) 88, 822, 951
BshFI GGCC 1 cut(s) 692
BsiHKAI GWGCWC 3 cut(s) 507, 596, 971
BsiHKCI CYCGRG 1 cut(s) 668
BsiSI CCGG 2 cut(s) 646, 669
BslI CCNNNNNNNGG 1 cut(s) 914
BsmAI GTCTC 1 cut(s) 190
BsmI GAATGC 1 cut(s) 952
BsnI GGCC 1 cut(s) 692
BsoBI CYCGRG 1 cut(s) 668
Bsp120I GGGCCC 1 cut(s) 690
Bsp1286I GDGCHC 4 cut(s) 507, 596, 694, 971
Bsp143I GATC 4 cut(s) 427, 706, 875, 883
BspACI CCGC 4 cut(s) 135, 522, 561, 710
BspANI GGCC 1 cut(s) 692
BspCNI CTCAG 1 cut(s) 853
BspLI GGNNCC 4 cut(s) 543, 692, 693, 885
BspPI GGATC 4 cut(s) 422, 701, 878, 891
BsrI ACTGG 2 cut(s) 502, 1007
BssECI CCNNGG 1 cut(s) 668
BssMI GATC 4 cut(s) 427, 706, 875, 883
BssSI CACGAG 2 cut(s) 30, 819
Bst2BI CACGAG 2 cut(s) 30, 819
Bst4CI ACNGT 2 cut(s) 62, 736
Bst6I CTCTTC 4 cut(s) 205, 235, 298, 404
BstAPI GCANNNNNTGC 1 cut(s) 73
BstC8I GCNNGC 5 cut(s) 503, 526, 586, 609, 714
BstDEI CTNAG 1 cut(s) 861
BstF5I GGATG 1 cut(s) 571
BstKTI GATC 4 cut(s) 430, 709, 878, 886
BstMAI GTCTC 1 cut(s) 190
BstMBI GATC 4 cut(s) 427, 706, 875, 883
BstMWI GCNNNNNNNGC 1 cut(s) 73
BstNSI RCATGY 3 cut(s) 329, 359, 377
BstPAI GACNNNNGTC 1 cut(s) 254
BstSCI CCNGG 3 cut(s) 645, 667, 668
BstSLI GKGCMC 1 cut(s) 694
BstV1I GCAGC 3 cut(s) 88, 822, 951
BstX2I RGATCY 3 cut(s) 427, 875, 883
BstYI RGATCY 3 cut(s) 427, 875, 883
BsuRI GGCC 1 cut(s) 692
BtsCI GGATG 1 cut(s) 571
Cac8I GCNNGC 5 cut(s) 503, 526, 586, 609, 714
CaiI CAGNNNCTG 1 cut(s) 1013
Cfr13I GGNCC 2 cut(s) 690, 691
Cfr9I CCCGGG 1 cut(s) 668
Csp6I GTAC 2 cut(s) 105, 703
CviAII CATG 4 cut(s) 326, 356, 374, 422
CviQI GTAC 2 cut(s) 105, 703
DdeI CTNAG 1 cut(s) 861
DpnI GATC 4 cut(s) 429, 708, 877, 885
DpnII GATC 4 cut(s) 427, 706, 875, 883
Eam1104I CTCTTC 4 cut(s) 205, 235, 298, 404
EarI CTCTTC 4 cut(s) 205, 235, 298, 404
EciI GGCGGA 1 cut(s) 699
Ecl136II GAGCTC 1 cut(s) 969
Eco24I GRGCYC 2 cut(s) 694, 971
Eco32I GATATC 1 cut(s) 226
Eco53kI GAGCTC 1 cut(s) 969
Eco88I CYCGRG 1 cut(s) 668
EcoICRI GAGCTC 1 cut(s) 969
EcoO109I RGGNCCY 1 cut(s) 691
EcoRV GATATC 1 cut(s) 226
EcoT38I GRGCYC 2 cut(s) 694, 971
FaeI CATG 4 cut(s) 329, 359, 377, 425
FaiI YATR 9 cut(s) 14, 20, 327, 357, 375, 390, 423, 530, 559
FatI CATG 4 cut(s) 325, 355, 373, 421
FauI CCCGC 1 cut(s) 554
FblI GTMKAC 4 cut(s) 57, 218, 248, 903
Fnu4HI GCNGC 3 cut(s) 77, 811, 940
FokI GGATG 1 cut(s) 578
FriOI GRGCYC 2 cut(s) 694, 971
Fsp4HI GCNGC 3 cut(s) 77, 811, 940
FspBI CTAG 1 cut(s) 108
GluI GCNGC 3 cut(s) 77, 811, 940
HaeIII GGCC 1 cut(s) 692
HapII CCGG 2 cut(s) 646, 669
Hin1II CATG 4 cut(s) 329, 359, 377, 425
HincII GTYRAC 3 cut(s) 219, 249, 904
HindII GTYRAC 3 cut(s) 219, 249, 904
HinfI GANTC 4 cut(s) 158, 245, 275, 338
HpaII CCGG 2 cut(s) 646, 669
HphI GGTGA 4 cut(s) 167, 569, 657, 935
Hpy166II GTNNAC 6 cut(s) 58, 88, 219, 249, 703, 904
Hpy188I TCNGA 3 cut(s) 382, 636, 862
Hpy188III TCNNGA 4 cut(s) 187, 819, 881, 976
Hpy8I GTNNAC 6 cut(s) 58, 88, 219, 249, 703, 904
HpyAV CCTTC 2 cut(s) 790, 982
HpyCH4III ACNGT 2 cut(s) 62, 736
HpyCH4IV ACGT 1 cut(s) 996
HpyCH4V TGCA 5 cut(s) 67, 76, 437, 479, 1000
HpyF10VI GCNNNNNNNGC 1 cut(s) 73
HpyF3I CTNAG 1 cut(s) 861
HpySE526I ACGT 1 cut(s) 996
Hsp92II CATG 4 cut(s) 329, 359, 377, 425
Kzo9I GATC 4 cut(s) 427, 706, 875, 883
LmnI GCTCC 3 cut(s) 616, 966, 974
Lsp1109I GCAGC 3 cut(s) 88, 822, 951
MaeI CTAG 1 cut(s) 108
MaeII ACGT 1 cut(s) 996
MaeIII GTNAC 4 cut(s) 451, 682, 736, 869
MalI GATC 4 cut(s) 429, 708, 877, 885
MboI GATC 4 cut(s) 427, 706, 875, 883
MboII GAAGA 8 cut(s) 131, 222, 252, 315, 421, 437, 784, 793
MfeI CAATTG 1 cut(s) 312
MflI RGATCY 3 cut(s) 427, 875, 883
MhlI GDGCHC 4 cut(s) 507, 596, 694, 971
MluCI AATT 4 cut(s) 99, 312, 775, 982
MlyI GAGTC 4 cut(s) 167, 254, 284, 347
MmeI TCCRAC 7 cut(s) 157, 187, 217, 247, 280, 310, 340
MseI TTAA 2 cut(s) 198, 291
MspI CCGG 2 cut(s) 646, 669
MspR9I CCNGG 3 cut(s) 647, 669, 670
MunI CAATTG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 952
MwoI GCNNNNNNNGC 1 cut(s) 73
NciI CCSGG 3 cut(s) 647, 669, 670
NdeII GATC 4 cut(s) 427, 706, 875, 883
NlaIII CATG 4 cut(s) 329, 359, 377, 425
NlaIV GGNNCC 4 cut(s) 543, 692, 693, 885
NmuCI GTSAC 3 cut(s) 451, 682, 869
NspI RCATGY 3 cut(s) 329, 359, 377
PciI ACATGT 2 cut(s) 325, 355
PctI GAATGC 1 cut(s) 952
PflMI CCANNNNNTGG 1 cut(s) 914
PkrI GCNGC 3 cut(s) 78, 812, 941
PleI GAGTC 4 cut(s) 166, 253, 283, 346
PpsI GAGTC 4 cut(s) 166, 253, 283, 346
PscI ACATGT 2 cut(s) 325, 355
PshAI GACNNNNGTC 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 971
PspN4I GGNNCC 4 cut(s) 543, 692, 693, 885
PspOMI GGGCCC 1 cut(s) 690
PspPI GGNCC 2 cut(s) 690, 691
PstNI CAGNNNCTG 1 cut(s) 1013
PsuI RGATCY 3 cut(s) 427, 875, 883
RsaI GTAC 2 cut(s) 106, 704
RsaNI GTAC 2 cut(s) 105, 703
SacI GAGCTC 1 cut(s) 971
SalI GTCGAC 3 cut(s) 217, 247, 902
SaqAI TTAA 2 cut(s) 198, 291
SatI GCNGC 3 cut(s) 77, 811, 940
Sau3AI GATC 4 cut(s) 427, 706, 875, 883
Sau96I GGNCC 2 cut(s) 690, 691
SchI GAGTC 4 cut(s) 167, 254, 284, 347
ScrFI CCNGG 3 cut(s) 647, 669, 670
SduI GDGCHC 4 cut(s) 507, 596, 694, 971
SmaI CCCGGG 1 cut(s) 670
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 4 cut(s) 99, 312, 775, 982
SsiI CCGC 4 cut(s) 135, 522, 561, 710
SspMI CTAG 1 cut(s) 108
SstI GAGCTC 1 cut(s) 971
StyD4I CCNGG 3 cut(s) 645, 667, 668
TaaI ACNGT 2 cut(s) 62, 736
TaiI ACGT 1 cut(s) 999
TaqI TCGA 7 cut(s) 188, 218, 248, 278, 341, 457, 903
TasI AATT 4 cut(s) 99, 312, 775, 982
Tru1I TTAA 2 cut(s) 198, 291
Tru9I TTAA 2 cut(s) 198, 291
TseFI GTSAC 3 cut(s) 451, 682, 869
TseI GCWGC 3 cut(s) 76, 810, 939
Tsp45I GTSAC 3 cut(s) 451, 682, 869
TspDTI ATGAA 2 cut(s) 438, 582
TspMI CCCGGG 1 cut(s) 668
Van91I CCANNNNNTGG 1 cut(s) 914
XapI RAATTY 2 cut(s) 99, 982
XceI RCATGY 3 cut(s) 329, 359, 377
XmaI CCCGGG 1 cut(s) 668
XmiI GTMKAC 4 cut(s) 57, 218, 248, 903
XspI CTAG 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.