Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004637

Physical Location & Seq

Genomic Coordinates Forward (+)

21964042 .. 21968849

Gene Structure 4808 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1132900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1677 bp

ATGGGGCTCACGGAGACTCAGCCGGGCCTCTCTCTAGGGTTTAGTTCGCATCAGAGTACGAGCTCGTCGAATTCAATCCAATCCGTTCCGCTGCAATTGCTCGACCAGAAAGGAGATAAGATTAGGGTTGAGAATGGGGATGATCGCGAAGAGGACGAGAAGTTCAATCTCTTGGGGCACTCTATCTGCCTGAAAAGACAGCGGGATGGGTTCGGAGTGAACCCGAACCCGTCAAAGTGCTTGGCGGTTGAATCGGCTGGGCTGGAGGCGAGGCGGGCCGCTGTTCGGTCTTGGGGGAACCAGCCCCTTTCGGTTGCGGACCCGGAGGTCCATGAAATTATGGAGAAGGAAAAACTTCGGCAGTTCAAAGGTATTGAATTGGTTGCTTCTGAGAATTTTGTGTGCAGAGCTGTGATGGAAGCTCTTGGCAGCCACTTGACGAACAAGTACTCCGAGGGTATGCCCGGAGCGAGATACTACACGGGCAATCAGCATATTGATCAGATTGAATTGCTATGTCATGAACGCGCATTGGCAGCTTTTGGTCTTGATCCTGAGAAATGGGGTGTCAATGTCCAGCCTTATTCTTGTACCTCTGCGAATATCGCAGTCTACACCGGTCTTTTGCTCCCAAATGATAGGATTATGGGCTTGGATTCGCCTTCGGGAGGGCATATGAGTCATGGGTATTATACTTCAGGTGGGAAGAAGGTAACGGCTGCCTCGATATTCTTTGATAGTTTCCCTTACAAGGTAAATCCGCAAACAGGGTACATTGATTTTGATAAGCTTGAGGAGAGGGCGGTTGATTATCGTCCCAAGATACTTATTTGCGGTGGTAGCTCATACCCCAGGGAGTGGGATTATGCCAGGTTTAGGCATATTGCAGATAAATGCGGAGCTGTGTTGATGTGTGACATGGCTCATATTAGCGGTCTTGTGGCAGCCAAGGAATGTGCAAGCCCGTTTGATTATTGTGATATTGTTACGTCAACAACGCACAAAAGTCTCCGGGGTCCTAGAGGAGGTATTATTTTTTACAGAAAAGGCACAAAATTAAGGAAGCAAGGTGTACATCATACTGATGAGGGTAGTAACAATTATGATTTAGAGGAAAAGATAAACTTTGCCGTTTTCCCCTCCCTACAAGGAGGTCCTCACAACAATTATATTGCTGCTCTTGCCATCGCCTTAAAACAAGTAGCTACTCCAGAGTATAAAGCATACATGAACCAGGTGAAGAGAAATGCACAGGCCTTGGCTAATGCTTTGTTAAGAAGAAAATGCAGATTGGTGACTGGGGGTACCGATAATCACTTGCTGCTTTGGGATCTGACTGGTCTTGGTATAACAGGTAAGAACTTTGAAAAGGTCTGCGAGTTGGCCGACATCACTCTCAATAAAACTGCTATATTTGGTGATAATGGTGCTCTTTCTCTTCGGGGAGTGAGAATTGGTACTCCTGCTATGACGACAAGAGGTTGTGTGGAGACCGATTTTGAGACAATTGCAGACTTTCTTCTAAGGGCTGCTCAGATCACTGCTTCTGTTCAGAGGGAACATGGAAAATTTCAGAAAGATTTTATGCAAGGTCTCCAGAACAACAAAGATATTGTTGAGCTCAGAAATCGGGTTGAAAAATTTGCGTACCAGTTTGAAATGCCAGGATATGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004372 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005759 GO:0006730 GO:0006732 GO:0006790 GO:0008150 GO:0008152 GO:0009987 GO:0010035 GO:0010038 GO:0016740 GO:0016741 GO:0016742 GO:0017144 GO:0019219 GO:0019222 GO:0031323 GO:0031974 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044030 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046500 GO:0046686 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051052 GO:0051171 GO:0051186 GO:0055063 GO:0055081 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0072505 GO:0080090 GO:0098771

KEGG Pathways

Metabolic & Signaling

ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523

Pfam Domains

Protein Families

SHMT

Protein Analysis

559

Amino Acids

61.83

Weight (kDa)

7.23

Isoelectric Point (pI)

39.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SHMT	PF00464	104 - 506	3.6e-170	Serine hydroxymethyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004637)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22020 AT1G22020 AT1G36370
fragaria_vesca	FvH4_5g28970 FvH4_5g38450 FvH4_5g38450
malus_domestica	MD04G1132900.v1.1 MD12G1145600.v1.1
prunus_persica	Prupe.1G507500_v2.0.a1 Prupe.1G507600_v2.0.a1
pyrus_communis	pycom04g12080 pycom12g13990 pycom15g32210
rosa_chinensis	RchiOBHm_Chr7g0221291 RchiOBHm_Chr7g0240491
rosa_laevigata	RLG00000000675 RLG00000002164
rosa_multiflora	Rmu_sc0000324.1_g000020 Rmu_sc0005327.1_g000009
rosa_roxburghii	Rroxscaffold_3G00219940 Rroxscaffold_3G00238440
rosa_rugosa	Rorug07G0202000 Rorug07G0336600
rosa_samantha	Rh7AG341100 Rh7AG491000 Rh7BG332100 Rh7BG463500 Rh7CG359000 Rh7CG507100
rosa_wichuraiana	Rw7G029010 Rw7G041140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	326
Acc65I	GGTACC	1 cut(s)	1304
AccB1I	GGYRCC	1 cut(s)	1304
AccB7I	CCANNNNNTGG	1 cut(s)	858
AccI	GTMKAC	1 cut(s)	612
AccII	CGCG	2 cut(s)	147, 528
AclWI	GGATC	2 cut(s)	545, 1338
AcoI	YGGCCR	1 cut(s)	1383
AcsI	RAATTY	4 cut(s)	70, 394, 1568, 1640
AcuI	CTGAAG	1 cut(s)	681
AfaI	GTAC	8 cut(s)	58, 449, 592, 773, 1074, 1306, 1459, 1649
AfiI	CCNNNNNNNGG	7 cut(s)	285, 668, 751, 767, 858, 876, 1025
AgeI	ACCGGT	1 cut(s)	617
AgsI	TTSAA	9 cut(s)	75, 166, 251, 367, 377, 509, 1367, 1637, 1658
AjnI	CCWGG	4 cut(s)	851, 869, 1233, 1663
AloI	GAACNNNNNNTCC	4 cut(s)	146, 178, 434, 466
AluBI	AGCT	9 cut(s)	63, 410, 422, 539, 790, 843, 902, 1205, 1621
AluI	AGCT	9 cut(s)	63, 410, 422, 539, 790, 843, 902, 1205, 1621
Alw21I	GWGCWC	3 cut(s)	65, 1432, 1623
Alw26I	GTCTC	5 cut(s)	8, 1013, 1484, 1496, 1598
AlwI	GGATC	2 cut(s)	545, 1338
AoxI	GGCC	4 cut(s)	25, 276, 1254, 1383
ApeKI	GCWGC	8 cut(s)	91, 429, 536, 719, 944, 1175, 1321, 1529
ApoI	RAATTY	4 cut(s)	70, 394, 1568, 1640
AsiGI	ACCGGT	1 cut(s)	617
Asp700I	GAANNNNTTC	1 cut(s)	354
Asp718I	GGTACC	1 cut(s)	1304
AspLEI	GCGC	1 cut(s)	530
AspS9I	GGNCC	6 cut(s)	25, 276, 319, 328, 1016, 1154
AsuC2I	CCSGG	4 cut(s)	24, 323, 465, 1013
AsuHPI	GGTGA	3 cut(s)	1249, 1306, 1430
AvaII	GGWCC	4 cut(s)	319, 328, 1016, 1154
BaeGI	GKGCMC	1 cut(s)	180
BanI	GGYRCC	1 cut(s)	1304
BanII	GRGCYC	3 cut(s)	9, 65, 1623
Bbv12I	GWGCWC	3 cut(s)	65, 1432, 1623
BbvI	GCAGC	8 cut(s)	78, 441, 548, 706, 956, 1162, 1308, 1516
BccI	CCATC	3 cut(s)	200, 409, 1193
BceAI	ACGGC	2 cut(s)	732, 1115
BciT130I	CCWGG	4 cut(s)	853, 871, 1235, 1665
BclI	TGATCA	1 cut(s)	499
BcnI	CCSGG	4 cut(s)	24, 323, 465, 1013
BcoDI	GTCTC	5 cut(s)	8, 1013, 1484, 1496, 1598
BfaI	CTAG	2 cut(s)	35, 1020
BisI	GCNGC	9 cut(s)	92, 279, 430, 537, 720, 945, 1176, 1322, 1530
BlsI	GCNGC	9 cut(s)	93, 280, 431, 538, 721, 946, 1177, 1323, 1531
BmcAI	AGTACT	1 cut(s)	449
Bme1390I	CCNGG	8 cut(s)	24, 323, 465, 853, 871, 1013, 1235, 1665
Bme18I	GGWCC	4 cut(s)	319, 328, 1016, 1154
BmgT120I	GGNCC	6 cut(s)	25, 276, 319, 328, 1016, 1154
BmiI	GGNNCC	4 cut(s)	299, 321, 1017, 1306
BmrFI	CCNGG	8 cut(s)	24, 323, 465, 853, 871, 1013, 1235, 1665
BmrI	ACTGGG	1 cut(s)	1308
BmsI	GCATC	1 cut(s)	58
BmuI	ACTGGG	1 cut(s)	1308
BpmI	CTGGAG	3 cut(s)	284, 1194, 1580
BpuEI	CTTGAG	1 cut(s)	812
BpuMI	CCSGG	4 cut(s)	24, 323, 465, 1013
BsaI	GGTCTC	2 cut(s)	1484, 1598
BsaJI	CCNNGG	6 cut(s)	453, 851, 852, 948, 1012, 1257
BsaWI	WCCGGW	1 cut(s)	617
BsaXI	ACNNNNNCTCC	2 cut(s)	434, 464
Bsc4I	CCNNNNNNNGG	7 cut(s)	285, 668, 751, 767, 858, 876, 1025
Bse118I	RCCGGY	1 cut(s)	617
Bse1I	ACTGG	3 cut(s)	1303, 1342, 1651
BseBI	CCWGG	4 cut(s)	853, 871, 1235, 1665
BseDI	CCNNGG	6 cut(s)	453, 851, 852, 948, 1012, 1257
BseGI	GGATG	2 cut(s)	145, 211
BseLI	CCNNNNNNNGG	7 cut(s)	285, 668, 751, 767, 858, 876, 1025
BseMII	CTCAG	5 cut(s)	32, 381, 546, 1547, 1636
BseNI	ACTGG	3 cut(s)	1303, 1342, 1651
BseRI	GAGGAG	2 cut(s)	809, 1038
BseSI	GKGCMC	1 cut(s)	180
BseXI	GCAGC	8 cut(s)	78, 441, 548, 706, 956, 1162, 1308, 1516
BseYI	CCCAGC	1 cut(s)	257
BsgI	GTGCAG	1 cut(s)	424
Bsh1236I	CGCG	2 cut(s)	147, 528
BshFI	GGCC	4 cut(s)	27, 278, 1256, 1385
BshNI	GGYRCC	1 cut(s)	1304
BshTI	ACCGGT	1 cut(s)	617
BsiHKAI	GWGCWC	3 cut(s)	65, 1432, 1623
BsiSI	CCGG	5 cut(s)	23, 323, 465, 618, 1012
BslFI	GGGAC	1 cut(s)	801
BslI	CCNNNNNNNGG	7 cut(s)	285, 668, 751, 767, 858, 876, 1025
BsmAI	GTCTC	5 cut(s)	8, 1013, 1484, 1496, 1598
BsmFI	GGGAC	1 cut(s)	801
BsnI	GGCC	4 cut(s)	27, 278, 1256, 1385
Bso31I	GGTCTC	2 cut(s)	1484, 1598
Bsp1286I	GDGCHC	5 cut(s)	9, 65, 180, 1432, 1623
Bsp1407I	TGTACA	1 cut(s)	1072
Bsp143I	GATC	5 cut(s)	142, 499, 550, 1330, 1536
Bsp68I	TCGCGA	1 cut(s)	147
BspANI	GGCC	4 cut(s)	27, 278, 1256, 1385
BspCNI	CTCAG	5 cut(s)	31, 382, 547, 1546, 1635
BspFNI	CGCG	2 cut(s)	147, 528
BspHI	TCATGA	1 cut(s)	520
BspLI	GGNNCC	4 cut(s)	299, 321, 1017, 1306
BspPI	GGATC	2 cut(s)	545, 1338
BspT107I	GGYRCC	1 cut(s)	1304
BspTNI	GGTCTC	2 cut(s)	1484, 1598
BsrFI	RCCGGY	1 cut(s)	617
BsrGI	TGTACA	1 cut(s)	1072
BsrI	ACTGG	3 cut(s)	1303, 1342, 1651
BssAI	RCCGGY	1 cut(s)	617
BssECI	CCNNGG	6 cut(s)	453, 851, 852, 948, 1012, 1257
BssMI	GATC	5 cut(s)	142, 499, 550, 1330, 1536
BssT1I	CCWWGG	2 cut(s)	948, 1257
Bst2UI	CCWGG	4 cut(s)	853, 871, 1235, 1665
Bst6I	CTCTTC	3 cut(s)	144, 1235, 1443
BstAUI	TGTACA	1 cut(s)	1072
BstC8I	GCNNGC	2 cut(s)	276, 961
BstDEI	CTNAG	6 cut(s)	18, 390, 555, 1523, 1533, 1622
BstENI	CCTNNNNNAGG	2 cut(s)	666, 1023
BstF5I	GGATG	2 cut(s)	145, 211
BstFNI	CGCG	2 cut(s)	147, 528
BstHHI	GCGC	1 cut(s)	530
BstKTI	GATC	5 cut(s)	145, 502, 553, 1333, 1539
BstMAI	GTCTC	5 cut(s)	8, 1013, 1484, 1496, 1598
BstMBI	GATC	5 cut(s)	142, 499, 550, 1330, 1536
BstMWI	GCNNNNNNNGC	6 cut(s)	97, 275, 536, 605, 840, 1181
BstNI	CCWGG	4 cut(s)	853, 871, 1235, 1665
BstSCI	CCNGG	8 cut(s)	22, 321, 463, 851, 869, 1011, 1233, 1663
BstSLI	GKGCMC	1 cut(s)	180
BstUI	CGCG	2 cut(s)	147, 528
BstV1I	GCAGC	8 cut(s)	78, 441, 548, 706, 956, 1162, 1308, 1516
BstX2I	RGATCY	1 cut(s)	1330
BstYI	RGATCY	1 cut(s)	1330
BsuRI	GGCC	4 cut(s)	27, 278, 1256, 1385
BtgZI	GCGATG	1 cut(s)	1171
BtsCI	GGATG	2 cut(s)	145, 211
BtsI	GCAGTG	1 cut(s)	1539
BtsIMutI	CAGTG	1 cut(s)	1539
BtuMI	TCGCGA	1 cut(s)	147
Cac8I	GCNNGC	2 cut(s)	276, 961
CciI	TCATGA	1 cut(s)	520
CfoI	GCGC	1 cut(s)	530
Cfr10I	RCCGGY	1 cut(s)	617
Cfr13I	GGNCC	6 cut(s)	25, 276, 319, 328, 1016, 1154
CsiI	ACCWGGT	1 cut(s)	1233
Csp6I	GTAC	8 cut(s)	57, 448, 591, 772, 1073, 1305, 1458, 1648
CspAI	ACCGGT	1 cut(s)	617
CviAII	CATG	6 cut(s)	332, 521, 683, 919, 1228, 1562
CviQI	GTAC	8 cut(s)	57, 448, 591, 772, 1073, 1305, 1458, 1648
DdeI	CTNAG	6 cut(s)	18, 390, 555, 1523, 1533, 1622
DpnI	GATC	5 cut(s)	144, 501, 552, 1332, 1538
DpnII	GATC	5 cut(s)	142, 499, 550, 1330, 1536
DrdI	GACNNNNNNGTC	1 cut(s)	326
DseDI	GACNNNNNNGTC	1 cut(s)	326
EaeI	YGGCCR	1 cut(s)	1383
Eam1104I	CTCTTC	3 cut(s)	144, 1235, 1443
EarI	CTCTTC	3 cut(s)	144, 1235, 1443
Ecl136II	GAGCTC	2 cut(s)	63, 1621
Eco130I	CCWWGG	2 cut(s)	948, 1257
Eco147I	AGGCCT	1 cut(s)	1256
Eco24I	GRGCYC	3 cut(s)	9, 65, 1623
Eco31I	GGTCTC	2 cut(s)	1484, 1598
Eco47I	GGWCC	4 cut(s)	319, 328, 1016, 1154
Eco53kI	GAGCTC	2 cut(s)	63, 1621
Eco57I	CTGAAG	1 cut(s)	681
EcoICRI	GAGCTC	2 cut(s)	63, 1621
EcoNI	CCTNNNNNAGG	2 cut(s)	666, 1023
EcoO109I	RGGNCCY	2 cut(s)	1016, 1154
EcoRI	GAATTC	1 cut(s)	70
EcoRII	CCWGG	4 cut(s)	851, 869, 1233, 1663
EcoT14I	CCWWGG	2 cut(s)	948, 1257
EcoT38I	GRGCYC	3 cut(s)	9, 65, 1623
ErhI	CCWWGG	2 cut(s)	948, 1257
FaeI	CATG	6 cut(s)	335, 524, 686, 922, 1231, 1565
FalI	AAGNNNNNCTT	2 cut(s)	1109, 1141
FaqI	GGGAC	1 cut(s)	801
FatI	CATG	6 cut(s)	331, 520, 682, 918, 1227, 1561
FauI	CCCGC	2 cut(s)	195, 267
FauNDI	CATATG	1 cut(s)	675
FbaI	TGATCA	1 cut(s)	499
FblI	GTMKAC	1 cut(s)	612
Fnu4HI	GCNGC	9 cut(s)	92, 279, 430, 537, 720, 945, 1176, 1322, 1530
FokI	GGATG	2 cut(s)	152, 218
FriOI	GRGCYC	3 cut(s)	9, 65, 1623
Fsp4HI	GCNGC	9 cut(s)	92, 279, 430, 537, 720, 945, 1176, 1322, 1530
FspBI	CTAG	2 cut(s)	35, 1020
GlaI	GCGC	1 cut(s)	529
GluI	GCNGC	9 cut(s)	92, 279, 430, 537, 720, 945, 1176, 1322, 1530
GsaI	CCCAGC	1 cut(s)	261
GsuI	CTGGAG	3 cut(s)	284, 1194, 1580
HaeIII	GGCC	4 cut(s)	27, 278, 1256, 1385
HapII	CCGG	5 cut(s)	23, 323, 465, 618, 1012
HhaI	GCGC	1 cut(s)	530
Hin1II	CATG	6 cut(s)	335, 524, 686, 922, 1231, 1565
Hin6I	GCGC	1 cut(s)	528
HinP1I	GCGC	1 cut(s)	528
HincII	GTYRAC	1 cut(s)	993
HindII	GTYRAC	1 cut(s)	993
HindIII	AAGCTT	1 cut(s)	788
HinfI	GANTC	4 cut(s)	16, 251, 656, 679
HpaII	CCGG	5 cut(s)	23, 323, 465, 618, 1012
HphI	GGTGA	3 cut(s)	1249, 1306, 1430
Hpy166II	GTNNAC	4 cut(s)	220, 613, 993, 1073
Hpy188III	TCNNGA	7 cut(s)	146, 521, 548, 554, 666, 1211, 1597
Hpy8I	GTNNAC	4 cut(s)	220, 613, 993, 1073
Hpy99I	CGWCG	1 cut(s)	70
HpyAV	CCTTC	3 cut(s)	340, 672, 703
HpyCH4IV	ACGT	1 cut(s)	989
HpyCH4V	TGCA	8 cut(s)	94, 405, 887, 959, 1250, 1287, 1511, 1588
HpyF10VI	GCNNNNNNNGC	6 cut(s)	97, 275, 536, 605, 840, 1181
HpyF3I	CTNAG	6 cut(s)	18, 390, 555, 1523, 1533, 1622
HpySE526I	ACGT	1 cut(s)	989
Hsp92II	CATG	6 cut(s)	335, 524, 686, 922, 1231, 1565
HspAI	GCGC	1 cut(s)	528
KpnI	GGTACC	1 cut(s)	1308
Ksp22I	TGATCA	1 cut(s)	499
Kzo9I	GATC	5 cut(s)	142, 499, 550, 1330, 1536
LmnI	GCTCC	3 cut(s)	467, 633, 899
Lsp1109I	GCAGC	8 cut(s)	78, 441, 548, 706, 956, 1162, 1308, 1516
LweI	GCATC	1 cut(s)	58
MabI	ACCWGGT	1 cut(s)	1233
MaeI	CTAG	2 cut(s)	35, 1020
MaeII	ACGT	1 cut(s)	989
MaeIII	GTNAC	5 cut(s)	712, 914, 985, 1094, 1294
MalI	GATC	5 cut(s)	144, 501, 552, 1332, 1538
MboI	GATC	5 cut(s)	142, 499, 550, 1330, 1536
MboII	GAAGA	6 cut(s)	161, 718, 1252, 1290, 1430, 1511
MfeI	CAATTG	2 cut(s)	95, 1506
MflI	RGATCY	1 cut(s)	1330
MhlI	GDGCHC	5 cut(s)	9, 65, 180, 1432, 1623
MlyI	GAGTC	2 cut(s)	10, 688
MroXI	GAANNNNTTC	1 cut(s)	354
MseI	TTAA	3 cut(s)	1058, 1193, 1274
MslI	CAYNNNNRTG	1 cut(s)	1083
MspA1I	CMGCKG	3 cut(s)	91, 202, 281
MspI	CCGG	5 cut(s)	23, 323, 465, 618, 1012
MspR9I	CCNGG	8 cut(s)	24, 323, 465, 853, 871, 1013, 1235, 1665
MunI	CAATTG	2 cut(s)	95, 1506
MvaI	CCWGG	4 cut(s)	853, 871, 1235, 1665
MvnI	CGCG	2 cut(s)	147, 528
MwoI	GCNNNNNNNGC	6 cut(s)	97, 275, 536, 605, 840, 1181
NciI	CCSGG	4 cut(s)	24, 323, 465, 1013
NdeI	CATATG	1 cut(s)	675
NdeII	GATC	5 cut(s)	142, 499, 550, 1330, 1536
NlaIII	CATG	6 cut(s)	335, 524, 686, 922, 1231, 1565
NlaIV	GGNNCC	4 cut(s)	299, 321, 1017, 1306
NmuCI	GTSAC	2 cut(s)	914, 1294
NruI	TCGCGA	1 cut(s)	147
PagI	TCATGA	1 cut(s)	520
PasI	CCCWGGG	1 cut(s)	852
PceI	AGGCCT	1 cut(s)	1256
PcsI	WCGNNNNNNNCGW	2 cut(s)	65, 722
PdmI	GAANNNNTTC	1 cut(s)	354
PfeI	GAWTC	2 cut(s)	251, 656
PflMI	CCANNNNNTGG	1 cut(s)	858
PinAI	ACCGGT	1 cut(s)	617
PkrI	GCNGC	9 cut(s)	93, 280, 431, 538, 721, 946, 1177, 1323, 1531
PleI	GAGTC	2 cut(s)	10, 687
PpsI	GAGTC	2 cut(s)	10, 687
PpuMI	RGGWCCY	2 cut(s)	1016, 1154
Psp124BI	GAGCTC	2 cut(s)	65, 1623
Psp5II	RGGWCCY	2 cut(s)	1016, 1154
Psp6I	CCWGG	4 cut(s)	851, 869, 1233, 1663
PspFI	CCCAGC	1 cut(s)	257
PspGI	CCWGG	4 cut(s)	851, 869, 1233, 1663
PspN4I	GGNNCC	4 cut(s)	299, 321, 1017, 1306
PspPI	GGNCC	6 cut(s)	25, 276, 319, 328, 1016, 1154
PspPPI	RGGWCCY	2 cut(s)	1016, 1154
PsuI	RGATCY	1 cut(s)	1330
RruI	TCGCGA	1 cut(s)	147
RsaI	GTAC	8 cut(s)	58, 449, 592, 773, 1074, 1306, 1459, 1649
RsaNI	GTAC	8 cut(s)	57, 448, 591, 772, 1073, 1305, 1458, 1648
RseI	CAYNNNNRTG	1 cut(s)	1083
SacI	GAGCTC	2 cut(s)	65, 1623
SaqAI	TTAA	3 cut(s)	1058, 1193, 1274
SatI	GCNGC	9 cut(s)	92, 279, 430, 537, 720, 945, 1176, 1322, 1530
Sau3AI	GATC	5 cut(s)	142, 499, 550, 1330, 1536
Sau96I	GGNCC	6 cut(s)	25, 276, 319, 328, 1016, 1154
ScaI	AGTACT	1 cut(s)	449
SchI	GAGTC	2 cut(s)	10, 688
ScrFI	CCNGG	8 cut(s)	24, 323, 465, 853, 871, 1013, 1235, 1665
SduI	GDGCHC	5 cut(s)	9, 65, 180, 1432, 1623
SexAI	ACCWGGT	1 cut(s)	1233
SfaNI	GCATC	1 cut(s)	58
SinI	GGWCC	4 cut(s)	319, 328, 1016, 1154
SmiMI	CAYNNNNRTG	1 cut(s)	1083
SmlI	CTYRAG	1 cut(s)	791
SmoI	CTYRAG	1 cut(s)	791
SseBI	AGGCCT	1 cut(s)	1256
SspMI	CTAG	2 cut(s)	35, 1020
SstI	GAGCTC	2 cut(s)	65, 1623
StuI	AGGCCT	1 cut(s)	1256
StyD4I	CCNGG	8 cut(s)	22, 321, 463, 851, 869, 1011, 1233, 1663
StyI	CCWWGG	2 cut(s)	948, 1257
TaiI	ACGT	1 cut(s)	992
TaqI	TCGA	3 cut(s)	68, 102, 725
TaqII	GACCGA	2 cut(s)	276, 1508
TatI	WGTACW	2 cut(s)	447, 1072
TauI	GCSGC	1 cut(s)	281
TfiI	GAWTC	2 cut(s)	251, 656
Tru1I	TTAA	3 cut(s)	1058, 1193, 1274
Tru9I	TTAA	3 cut(s)	1058, 1193, 1274
TscAI	CASTG	1 cut(s)	1546
TseFI	GTSAC	2 cut(s)	914, 1294
TseI	GCWGC	8 cut(s)	91, 429, 536, 719, 944, 1175, 1321, 1529
Tsp45I	GTSAC	2 cut(s)	914, 1294
TspDTI	ATGAA	3 cut(s)	348, 537, 1244
TspGWI	ACGGA	2 cut(s)	26, 73
TspRI	CASTG	1 cut(s)	1546
Van91I	CCANNNNNTGG	1 cut(s)	858
VpaK11BI	GGWCC	4 cut(s)	319, 328, 1016, 1154
XagI	CCTNNNNNAGG	2 cut(s)	666, 1023
XapI	RAATTY	4 cut(s)	70, 394, 1568, 1640
XmiI	GTMKAC	1 cut(s)	612
XmnI	GAANNNNTTC	1 cut(s)	354
XspI	CTAG	2 cut(s)	35, 1020
ZrmI	AGTACT	1 cut(s)	449

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.