Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0004637

Physical Location & Seq

Genomic Coordinates Forward (+)

34431778 .. 34436908

Gene Structure 5131 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG332100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1440 bp

ATGGACTTATCATCATCCTCCCACCCGCAATCCAACCTCTCTTTGGGATTTTCATCCCACGCGCCGTCGTCTCAAATCGCCGATGACTCCATCGCACTCCCCGTAGCCCCATCAATCCCGCTTCAATTGCTCGAGCCACAGCCAGAGAGCCACGCAACAACAACAACAACTAATGGTGAAATTGGCGAAGACGACGACGACGACAGAGACGTCGAGGAGTTTCGGATATTGGGGCATTCCATGTGCTTGAAACGCCGCAGGGACAGAGAATCCTCCTCCTGTTCCTCCACTAATTCCAAACGCGCGGCACTCTCACCGGACCTTGAGGCCCGGAGGACTGCTGTCCGGGCCTGGGGTGACCAGCCTCTCCGAGTTGCCGACTCCGATGTGTTTGAGATTATGGACAAGGAGAAGCAGAGGCAGTTCAAAGGGATTGAATTGATTGCCTCGGAGAATTTCGTGTGCCGGGCAGTAATGGAAGCACTTGGAAGCCACTTGACCAATAAGTATTCCGAAGGAATGCCCGGTGCAAGGTACTACGGTGGTAACCAGTACATTGATGAGATCGAAACACTTTGCTGTAAGCGTGCTTTAGCCGCATTCGGTCTCGATGCTGAGAATTGGGGTGTCAATGTGCAGCCTTATTCATGTACATCTGCCAACTTTGCTGTGTACACTGGATTGTTATTGCCCGGTGACAGAATAATGGGGTTGGATACCCCATCTGGAGGAAATACTAGTCATGGATATTATACCAGCAATGGGAGGAAAGTTTCCGGAGCATCAATTTTCTTTGAGACCTTGCCTTATAAGGTCAATTTACAAACTGGGTATATTGATTATGATAAGCTTGAGGAGAGGGCGCTTGATTTTCGCCCCAAGATACTTATTTGTGGTGGGAGCTCGTATCCCCGCGAATGGGATTATGCAAGGTTTAGGCAGATTGCTGATAAATGTGGAGCTGTGCTGCTGTGTGATATGGCGCAAATTAGTGGGCTTATTGCAGCAAAGGAATGTGTAAATCCTTTTGACTACTGTGACATTGTTACTTCAACTACTCATAAAAGCCTTCGGGGTCCCAGGGGAGGTATAATTTTTTATAGGAAGGGTACAAAACCAAGGAAGAGGGGATTTCTTTTAACTCAAGGAAGTGAAAGCGACCACTATGATTTTGAGGAAAAGATCAACTTTGCCGTTTTTCCATCATTGCAAGGGGGACCGCACAATAACCACATTGCTGCTCTTGCCATAGCTTTAAAGCAAGTGGCTACACCTGAATACAAGGCATATATGCAGCAAGTGAAGAAAAATGCACAGGCTTTAGCATCATCTTTGCTGAGAAGAAAGTGCAGGTTAGTAACCGGTGGCACAGACAACCATTTGCTACTTTGGGATCTGAGGCCCCTAGGATTATCAGGTATAGTCTTTTCTAAAATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004372 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006730 GO:0008150 GO:0008152 GO:0009987 GO:0016740 GO:0016741 GO:0016742 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523

Pfam Domains

Protein Families

SHMT

Protein Analysis

479

Amino Acids

52.91

Weight (kDa)

6.61

Isoelectric Point (pI)

46.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SHMT	PF00464	124 - 475	4.9e-150	Serine hydroxymethyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004637)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22020 AT1G22020 AT1G36370
fragaria_vesca	FvH4_5g28970 FvH4_5g38450 FvH4_5g38450
malus_domestica	MD04G1132900.v1.1 MD12G1145600.v1.1
prunus_persica	Prupe.1G507500_v2.0.a1 Prupe.1G507600_v2.0.a1
pyrus_communis	pycom04g12080 pycom12g13990 pycom15g32210
rosa_chinensis	RchiOBHm_Chr7g0221291 RchiOBHm_Chr7g0240491
rosa_laevigata	RLG00000000675 RLG00000002164
rosa_multiflora	Rmu_sc0000324.1_g000020 Rmu_sc0005327.1_g000009
rosa_roxburghii	Rroxscaffold_3G00219940 Rroxscaffold_3G00238440
rosa_rugosa	Rorug07G0202000 Rorug07G0336600
rosa_samantha	Rh7AG341100 Rh7AG491000 Rh7BG332100 Rh7BG463500 Rh7CG359000 Rh7CG507100
rosa_wichuraiana	Rw7G029010 Rw7G041140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	810
AasI	GACNNNNNNGTC	1 cut(s)	209
AatII	GACGTC	1 cut(s)	213
Acc36I	ACCTGC	1 cut(s)	1341
AccII	CGCG	4 cut(s)	62, 303, 305, 915
AccIII	TCCGGA	1 cut(s)	776
AciI	CCGC	7 cut(s)	26, 119, 256, 305, 597, 913, 1220
AclWI	GGATC	1 cut(s)	1401
AcsI	RAATTY	1 cut(s)	454
AcyI	GRCGYC	1 cut(s)	210
AfaI	GTAC	5 cut(s)	536, 554, 652, 674, 1111
AfiI	CCNNNNNNNGG	8 cut(s)	43, 352, 531, 728, 762, 918, 919, 1085
AgeI	ACCGGT	1 cut(s)	1361
AgsI	TTSAA	5 cut(s)	125, 250, 427, 437, 1053
AhlI	ACTAGT	1 cut(s)	737
AjnI	CCWGG	2 cut(s)	350, 1079
AluBI	AGCT	4 cut(s)	850, 903, 962, 1253
AluI	AGCT	4 cut(s)	850, 903, 962, 1253
Alw21I	GWGCWC	1 cut(s)	905
Alw26I	GTCTC	4 cut(s)	75, 201, 611, 791
AlwI	GGATC	1 cut(s)	1401
Ama87I	CYCGRG	1 cut(s)	131
Aor13HI	TCCGGA	1 cut(s)	776
AoxI	GGCC	3 cut(s)	327, 348, 1400
ApeKI	GCWGC	5 cut(s)	637, 967, 1004, 1238, 1294
ApoI	RAATTY	1 cut(s)	454
AsiGI	ACCGGT	1 cut(s)	1361
AspA2I	CCTAGG	1 cut(s)	1405
AspLEI	GCGC	4 cut(s)	64, 305, 865, 985
AspS9I	GGNCC	6 cut(s)	319, 328, 348, 1076, 1217, 1401
AsuC2I	CCSGG	5 cut(s)	331, 347, 467, 525, 693
AsuHPI	GGTGA	4 cut(s)	188, 306, 368, 707
AvaI	CYCGRG	1 cut(s)	131
AvaII	GGWCC	3 cut(s)	319, 1076, 1217
AvrII	CCTAGG	1 cut(s)	1405
BaeI	ACNNNNGTAYC	2 cut(s)	526, 559
BanII	GRGCYC	1 cut(s)	905
BbsI	GAAGAC	1 cut(s)	195
Bbv12I	GWGCWC	1 cut(s)	905
BbvI	GCAGC	5 cut(s)	649, 954, 1016, 1225, 1306
BccI	CCATC	4 cut(s)	98, 118, 730, 1210
BceAI	ACGGC	2 cut(s)	49, 1178
BciT130I	CCWGG	2 cut(s)	352, 1081
BciVI	GTATCC	2 cut(s)	709, 918
BcnI	CCSGG	5 cut(s)	331, 347, 467, 525, 693
BcoDI	GTCTC	4 cut(s)	75, 201, 611, 791
BcuI	ACTAGT	1 cut(s)	737
BfaI	CTAG	2 cut(s)	738, 1406
BfoI	RGCGCY	1 cut(s)	866
BfuAI	ACCTGC	1 cut(s)	1341
BfuI	GTATCC	2 cut(s)	709, 918
BisI	GCNGC	8 cut(s)	256, 306, 597, 638, 968, 1005, 1239, 1295
BlnI	CCTAGG	1 cut(s)	1405
BlsI	GCNGC	8 cut(s)	257, 307, 598, 639, 969, 1006, 1240, 1296
Bme1390I	CCNGG	7 cut(s)	331, 347, 352, 467, 525, 693, 1081
Bme18I	GGWCC	3 cut(s)	319, 1076, 1217
BmeT110I	CYCGRG	1 cut(s)	131
BmgT120I	GGNCC	6 cut(s)	319, 328, 348, 1076, 1217, 1401
BmiI	GGNNCC	4 cut(s)	1077, 1078, 1218, 1403
BmrFI	CCNGG	7 cut(s)	331, 347, 352, 467, 525, 693, 1081
BmrI	ACTGGG	1 cut(s)	837
BmsI	GCATC	3 cut(s)	601, 791, 1334
BmuI	ACTGGG	1 cut(s)	837
BoxI	GACNNNNGTC	1 cut(s)	341
BpiI	GAAGAC	1 cut(s)	195
BpmI	CTGGAG	1 cut(s)	747
BpuEI	CTTGAG	3 cut(s)	344, 872, 1128
BpuMI	CCSGG	5 cut(s)	331, 347, 467, 525, 693
BsaBI	GATNNNNATC	1 cut(s)	52
BsaHI	GRCGYC	1 cut(s)	210
BsaI	GGTCTC	2 cut(s)	611, 791
BsaJI	CCNNGG	6 cut(s)	351, 447, 1079, 1080, 1118, 1405
BsaWI	WCCGGW	3 cut(s)	316, 776, 1361
Bsc4I	CCNNNNNNNGG	8 cut(s)	43, 352, 531, 728, 762, 918, 919, 1085
Bse118I	RCCGGY	1 cut(s)	1361
Bse1I	ACTGG	3 cut(s)	550, 682, 832
Bse3DI	GCAATG	3 cut(s)	766, 1205, 1233
Bse8I	GATNNNNATC	1 cut(s)	52
BseAI	TCCGGA	1 cut(s)	776
BseBI	CCWGG	2 cut(s)	352, 1081
BseDI	CCNNGG	6 cut(s)	351, 447, 1079, 1080, 1118, 1405
BseGI	GGATG	2 cut(s)	14, 53
BseJI	GATNNNNATC	1 cut(s)	52
BseLI	CCNNNNNNNGG	8 cut(s)	43, 352, 531, 728, 762, 918, 919, 1085
BseMI	GCAATG	3 cut(s)	766, 1205, 1233
BseMII	CTCAG	3 cut(s)	606, 1328, 1388
BseNI	ACTGG	3 cut(s)	550, 682, 832
BseRI	GAGGAG	3 cut(s)	230, 265, 869
BseXI	GCAGC	5 cut(s)	649, 954, 1016, 1225, 1306
BsgI	GTGCAG	2 cut(s)	656, 1369
Bsh1236I	CGCG	4 cut(s)	62, 303, 305, 915
BshFI	GGCC	3 cut(s)	329, 350, 1402
BshTI	ACCGGT	1 cut(s)	1361
BsiHKAI	GWGCWC	1 cut(s)	905
BsiHKCI	CYCGRG	1 cut(s)	131
BsiSI	CCGG	8 cut(s)	317, 331, 346, 466, 525, 693, 777, 1362
BslFI	GGGAC	3 cut(s)	275, 1062, 1230
BslI	CCNNNNNNNGG	8 cut(s)	43, 352, 531, 728, 762, 918, 919, 1085
BsmAI	GTCTC	4 cut(s)	75, 201, 611, 791
BsmBI	CGTCTC	2 cut(s)	75, 201
BsmFI	GGGAC	3 cut(s)	275, 1062, 1230
BsmI	GAATGC	3 cut(s)	235, 525, 599
BsnI	GGCC	3 cut(s)	329, 350, 1402
Bso31I	GGTCTC	2 cut(s)	611, 791
BsoBI	CYCGRG	1 cut(s)	131
Bsp1286I	GDGCHC	1 cut(s)	905
Bsp13I	TCCGGA	1 cut(s)	776
Bsp1407I	TGTACA	2 cut(s)	650, 672
Bsp143I	GATC	3 cut(s)	564, 1182, 1393
BspACI	CCGC	7 cut(s)	26, 119, 256, 305, 597, 913, 1220
BspANI	GGCC	3 cut(s)	329, 350, 1402
BspCNI	CTCAG	3 cut(s)	607, 1329, 1389
BspEI	TCCGGA	1 cut(s)	776
BspFNI	CGCG	4 cut(s)	62, 303, 305, 915
BspLI	GGNNCC	4 cut(s)	1077, 1078, 1218, 1403
BspMI	ACCTGC	1 cut(s)	1341
BspPI	GGATC	1 cut(s)	1401
BspTNI	GGTCTC	2 cut(s)	611, 791
BsrDI	GCAATG	3 cut(s)	766, 1205, 1233
BsrFI	RCCGGY	1 cut(s)	1361
BsrGI	TGTACA	2 cut(s)	650, 672
BsrI	ACTGG	3 cut(s)	550, 682, 832
BssAI	RCCGGY	1 cut(s)	1361
BssECI	CCNNGG	6 cut(s)	351, 447, 1079, 1080, 1118, 1405
BssMI	GATC	3 cut(s)	564, 1182, 1393
BssNI	GRCGYC	1 cut(s)	210
BssT1I	CCWWGG	2 cut(s)	1118, 1405
Bst2UI	CCWGG	2 cut(s)	352, 1081
Bst4CI	ACNGT	2 cut(s)	542, 1037
Bst6I	CTCTTC	1 cut(s)	1118
BstACI	GRCGYC	1 cut(s)	210
BstAUI	TGTACA	2 cut(s)	650, 672
BstC8I	GCNNGC	1 cut(s)	588
BstDEI	CTNAG	3 cut(s)	615, 1337, 1397
BstEII	GGTNACC	2 cut(s)	356, 545
BstF5I	GGATG	2 cut(s)	14, 53
BstFNI	CGCG	4 cut(s)	62, 303, 305, 915
BstH2I	RGCGCY	1 cut(s)	866
BstHHI	GCGC	4 cut(s)	64, 305, 865, 985
BstKTI	GATC	3 cut(s)	567, 1185, 1396
BstMAI	GTCTC	4 cut(s)	75, 201, 611, 791
BstMBI	GATC	3 cut(s)	564, 1182, 1393
BstMWI	GCNNNNNNNGC	6 cut(s)	127, 252, 347, 596, 665, 1244
BstNI	CCWGG	2 cut(s)	352, 1081
BstPAI	GACNNNNGTC	1 cut(s)	341
BstPI	GGTNACC	2 cut(s)	356, 545
BstSCI	CCNGG	7 cut(s)	329, 345, 350, 465, 523, 691, 1079
BstUI	CGCG	4 cut(s)	62, 303, 305, 915
BstV1I	GCAGC	5 cut(s)	649, 954, 1016, 1225, 1306
BstV2I	GAAGAC	1 cut(s)	195
BstX2I	RGATCY	1 cut(s)	1393
BstYI	RGATCY	1 cut(s)	1393
BsuI	GTATCC	2 cut(s)	709, 918
BsuRI	GGCC	3 cut(s)	329, 350, 1402
BtgZI	GCGATG	1 cut(s)	76
BtsCI	GGATG	2 cut(s)	14, 53
BtsIMutI	CAGTG	1 cut(s)	675
BveI	ACCTGC	1 cut(s)	1341
Cac8I	GCNNGC	1 cut(s)	588
CfoI	GCGC	4 cut(s)	64, 305, 865, 985
Cfr10I	RCCGGY	1 cut(s)	1361
Cfr13I	GGNCC	6 cut(s)	319, 328, 348, 1076, 1217, 1401
Csp6I	GTAC	5 cut(s)	535, 553, 651, 673, 1110
CspAI	ACCGGT	1 cut(s)	1361
CviAII	CATG	3 cut(s)	241, 648, 743
CviQI	GTAC	5 cut(s)	535, 553, 651, 673, 1110
DdeI	CTNAG	3 cut(s)	615, 1337, 1397
DpnI	GATC	3 cut(s)	566, 1184, 1395
DpnII	GATC	3 cut(s)	564, 1182, 1393
DraI	TTTAAA	1 cut(s)	1257
DrdI	GACNNNNNNGTC	1 cut(s)	209
DseDI	GACNNNNNNGTC	1 cut(s)	209
Eam1104I	CTCTTC	1 cut(s)	1118
EarI	CTCTTC	1 cut(s)	1118
Ecl136II	GAGCTC	1 cut(s)	903
Eco130I	CCWWGG	2 cut(s)	1118, 1405
Eco24I	GRGCYC	1 cut(s)	905
Eco31I	GGTCTC	2 cut(s)	611, 791
Eco47I	GGWCC	3 cut(s)	319, 1076, 1217
Eco53kI	GAGCTC	1 cut(s)	903
Eco88I	CYCGRG	1 cut(s)	131
Eco91I	GGTNACC	2 cut(s)	356, 545
EcoICRI	GAGCTC	1 cut(s)	903
EcoO109I	RGGNCCY	2 cut(s)	1076, 1401
EcoO65I	GGTNACC	2 cut(s)	356, 545
EcoRII	CCWGG	2 cut(s)	350, 1079
EcoT14I	CCWWGG	2 cut(s)	1118, 1405
EcoT38I	GRGCYC	1 cut(s)	905
ErhI	CCWWGG	2 cut(s)	1118, 1405
Esp3I	CGTCTC	2 cut(s)	75, 201
FaeI	CATG	3 cut(s)	244, 651, 746
FalI	AAGNNNNNCTT	2 cut(s)	1172, 1204
FaqI	GGGAC	3 cut(s)	275, 1062, 1230
FatI	CATG	3 cut(s)	240, 647, 742
FauI	CCCGC	3 cut(s)	33, 126, 920
Fnu4HI	GCNGC	8 cut(s)	256, 306, 597, 638, 968, 1005, 1239, 1295
FokI	GGATG	1 cut(s)	40
FriOI	GRGCYC	1 cut(s)	905
Fsp4HI	GCNGC	8 cut(s)	256, 306, 597, 638, 968, 1005, 1239, 1295
FspBI	CTAG	2 cut(s)	738, 1406
GlaI	GCGC	4 cut(s)	63, 304, 864, 984
GluI	GCNGC	8 cut(s)	256, 306, 597, 638, 968, 1005, 1239, 1295
GsuI	CTGGAG	1 cut(s)	747
HaeII	RGCGCY	1 cut(s)	866
HaeIII	GGCC	3 cut(s)	329, 350, 1402
HapII	CCGG	8 cut(s)	317, 331, 346, 466, 525, 693, 777, 1362
HhaI	GCGC	4 cut(s)	64, 305, 865, 985
Hin1I	GRCGYC	1 cut(s)	210
Hin1II	CATG	3 cut(s)	244, 651, 746
Hin6I	GCGC	4 cut(s)	62, 303, 863, 983
HinP1I	GCGC	4 cut(s)	62, 303, 863, 983
HindIII	AAGCTT	1 cut(s)	848
HinfI	GANTC	3 cut(s)	86, 269, 380
HpaII	CCGG	8 cut(s)	317, 331, 346, 466, 525, 693, 777, 1362
HphI	GGTGA	4 cut(s)	188, 306, 368, 707
Hpy166II	GTNNAC	2 cut(s)	673, 675
Hpy188I	TCNGA	6 cut(s)	225, 371, 385, 451, 514, 1398
Hpy188III	TCNNGA	3 cut(s)	608, 726, 777
Hpy8I	GTNNAC	2 cut(s)	673, 675
Hpy99I	CGWCG	5 cut(s)	70, 197, 200, 203, 215
HpyAV	CCTTC	3 cut(s)	509, 1079, 1099
HpyCH4III	ACNGT	2 cut(s)	542, 1037
HpyCH4IV	ACGT	1 cut(s)	210
HpyCH4V	TGCA	8 cut(s)	530, 637, 929, 1004, 1210, 1294, 1313, 1350
HpyF10VI	GCNNNNNNNGC	6 cut(s)	127, 252, 347, 596, 665, 1244
HpyF3I	CTNAG	3 cut(s)	615, 1337, 1397
HpySE526I	ACGT	1 cut(s)	210
Hsp92I	GRCGYC	1 cut(s)	210
Hsp92II	CATG	3 cut(s)	244, 651, 746
HspAI	GCGC	4 cut(s)	62, 303, 863, 983
KflI	GGGWCCC	1 cut(s)	1076
Kpn2I	TCCGGA	1 cut(s)	776
Kzo9I	GATC	3 cut(s)	564, 1182, 1393
LmnI	GCTCC	3 cut(s)	779, 900, 959
Lsp1109I	GCAGC	5 cut(s)	649, 954, 1016, 1225, 1306
LweI	GCATC	3 cut(s)	601, 791, 1334
MaeI	CTAG	2 cut(s)	738, 1406
MaeII	ACGT	1 cut(s)	210
MaeIII	GTNAC	6 cut(s)	356, 545, 695, 1037, 1045, 1357
MalI	GATC	3 cut(s)	566, 1184, 1395
MboI	GATC	3 cut(s)	564, 1182, 1393
MboII	GAAGA	4 cut(s)	200, 1135, 1315, 1353
MfeI	CAATTG	1 cut(s)	125
MflI	RGATCY	1 cut(s)	1393
MhlI	GDGCHC	1 cut(s)	905
MlyI	GAGTC	2 cut(s)	80, 374
MmeI	TCCRAC	2 cut(s)	57, 693
MroI	TCCGGA	1 cut(s)	776
MseI	TTAA	2 cut(s)	1139, 1256
MspI	CCGG	8 cut(s)	317, 331, 346, 466, 525, 693, 777, 1362
MspR9I	CCNGG	7 cut(s)	331, 347, 352, 467, 525, 693, 1081
MunI	CAATTG	1 cut(s)	125
Mva1269I	GAATGC	3 cut(s)	235, 525, 599
MvaI	CCWGG	2 cut(s)	352, 1081
MvnI	CGCG	4 cut(s)	62, 303, 305, 915
MwoI	GCNNNNNNNGC	6 cut(s)	127, 252, 347, 596, 665, 1244
NciI	CCSGG	5 cut(s)	331, 347, 467, 525, 693
NdeII	GATC	3 cut(s)	564, 1182, 1393
NlaIII	CATG	3 cut(s)	244, 651, 746
NlaIV	GGNNCC	4 cut(s)	1077, 1078, 1218, 1403
NmuCI	GTSAC	3 cut(s)	356, 695, 1037
PaeR7I	CTCGAG	1 cut(s)	131
PasI	CCCWGGG	1 cut(s)	1080
PcsI	WCGNNNNNNNCGW	3 cut(s)	99, 198, 207
PctI	GAATGC	3 cut(s)	235, 525, 599
PfeI	GAWTC	1 cut(s)	269
PinAI	ACCGGT	1 cut(s)	1361
PkrI	GCNGC	8 cut(s)	257, 307, 598, 639, 969, 1006, 1240, 1296
PleI	GAGTC	2 cut(s)	80, 374
PpsI	GAGTC	2 cut(s)	80, 374
PpuMI	RGGWCCY	1 cut(s)	1076
PshAI	GACNNNNGTC	1 cut(s)	341
PsiI	TTATAA	1 cut(s)	810
Psp124BI	GAGCTC	1 cut(s)	905
Psp5II	RGGWCCY	1 cut(s)	1076
Psp6I	CCWGG	2 cut(s)	350, 1079
PspEI	GGTNACC	2 cut(s)	356, 545
PspGI	CCWGG	2 cut(s)	350, 1079
PspN4I	GGNNCC	4 cut(s)	1077, 1078, 1218, 1403
PspPI	GGNCC	6 cut(s)	319, 328, 348, 1076, 1217, 1401
PspPPI	RGGWCCY	1 cut(s)	1076
PspXI	VCTCGAGB	1 cut(s)	131
PsuI	RGATCY	1 cut(s)	1393
RsaI	GTAC	5 cut(s)	536, 554, 652, 674, 1111
RsaNI	GTAC	5 cut(s)	535, 553, 651, 673, 1110
SacI	GAGCTC	1 cut(s)	905
SaqAI	TTAA	2 cut(s)	1139, 1256
SatI	GCNGC	8 cut(s)	256, 306, 597, 638, 968, 1005, 1239, 1295
Sau3AI	GATC	3 cut(s)	564, 1182, 1393
Sau96I	GGNCC	6 cut(s)	319, 328, 348, 1076, 1217, 1401
SchI	GAGTC	2 cut(s)	80, 374
ScrFI	CCNGG	7 cut(s)	331, 347, 352, 467, 525, 693, 1081
SduI	GDGCHC	1 cut(s)	905
SfaNI	GCATC	3 cut(s)	601, 791, 1334
Sfr274I	CTCGAG	1 cut(s)	131
SinI	GGWCC	3 cut(s)	319, 1076, 1217
SlaI	CTCGAG	1 cut(s)	131
SmlI	CTYRAG	4 cut(s)	131, 323, 851, 1143
SmoI	CTYRAG	4 cut(s)	131, 323, 851, 1143
SpeI	ACTAGT	1 cut(s)	737
SsiI	CCGC	7 cut(s)	26, 119, 256, 305, 597, 913, 1220
SspMI	CTAG	2 cut(s)	738, 1406
SstI	GAGCTC	1 cut(s)	905
StyD4I	CCNGG	7 cut(s)	329, 345, 350, 465, 523, 691, 1079
StyI	CCWWGG	2 cut(s)	1118, 1405
TaaI	ACNGT	2 cut(s)	542, 1037
TaiI	ACGT	1 cut(s)	213
TaqI	TCGA	4 cut(s)	132, 213, 567, 609
TaqII	GACCGA	1 cut(s)	593
TatI	WGTACW	3 cut(s)	552, 650, 672
TauI	GCSGC	3 cut(s)	258, 308, 599
TfiI	GAWTC	1 cut(s)	269
Tru1I	TTAA	2 cut(s)	1139, 1256
Tru9I	TTAA	2 cut(s)	1139, 1256
TscAI	CASTG	1 cut(s)	682
TseFI	GTSAC	3 cut(s)	356, 695, 1037
TseI	GCWGC	5 cut(s)	637, 967, 1004, 1238, 1294
Tsp45I	GTSAC	3 cut(s)	356, 695, 1037
TspDTI	ATGAA	2 cut(s)	42, 636
TspRI	CASTG	1 cut(s)	682
VpaK11BI	GGWCC	3 cut(s)	319, 1076, 1217
XapI	RAATTY	1 cut(s)	454
XcmI	CCANNNNNNNNNTGG	1 cut(s)	40
XhoI	CTCGAG	1 cut(s)	131
XmaJI	CCTAGG	1 cut(s)	1405
XspI	CTAG	2 cut(s)	738, 1406
ZraI	GACGTC	1 cut(s)	211

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.