pycom04g12080

Serine hydroxymethyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0004637
Physical Location & Seq
Forward (+)
15028281 .. 15028958
678 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g12080.1

Sequence Viewer

Length: 678 bp
ATGGGGCTCACGGAGGCTCAGCCGGGCCTCTCTCTAGGGTTTAGTTCGCATCAGAGTACGAGCTCGTCGAATTCAATCCAATCCGTTCCGCTGCAATTGCTCGACCAGAAAGGAGATAGGATTACGGTTGAGAATGGGGATGATCGCGAAGAGGACGAGAAGTTCAACCTCTTGGGGCACTCTATCTGCCTGAAAAGGCAGCGGGATGGGTTCGGAGTGAACCCGAACCCGTCAAAGTGCTTGGCGGTTGAATCGGCTGGGCTGGAGGCGAGGCGGGCCGCTGTTCGGTCTTGGGGGAACCAGCCCCTTTCGGTTGCGGACCCGGAGGTCCATGAAATTATGGAGAAGGAAAAACATCGGCAGTTCAAAGGTATTGAATTGGTTGCTTCTGAGAATTTTGTGTGCAGAGCTGTGATGGAAGCTCTTGGCAGCCACTTGACGAACAAGTACTCTGAGGGTATGCCCGGAGCGAGATACTACACGGGCAATCAGCATATTGATCGATCGAATTGCTATGTCAAGAACGCGCATTGGCAGCTTTTGGTCTTGATCCAGAGAAATGGGGTGTCAATGTCCAGCCTTATTCTTGTACCTCGGCGAATATCGCAGTCTACACCGGTCTTTTGCTCCCAAATGATAGGATTATGGGCTTGGATTCGCCTTCGGGAGGGCATATGA

Protein Analysis

226

Amino Acids

25.09

Weight (kDa)

7.7

Isoelectric Point (pI)

49.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SHMT PF00464 104 - 174 6e-27 Serine hydroxymethyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 326
AccI GTMKAC 1 cut(s) 611
AccII CGCG 2 cut(s) 147, 527
AciI CCGC 6 cut(s) 89, 202, 245, 274, 279, 317
AclWI GGATC 1 cut(s) 544
AcsI RAATTY 2 cut(s) 70, 394
AfaI GTAC 3 cut(s) 58, 449, 591
AfiI CCNNNNNNNGG 2 cut(s) 285, 667
AgeI ACCGGT 1 cut(s) 616
AgsI TTSAA 5 cut(s) 75, 166, 251, 367, 377
AloI GAACNNNNNNTCC 2 cut(s) 146, 178
AluBI AGCT 4 cut(s) 63, 410, 422, 538
AluI AGCT 4 cut(s) 63, 410, 422, 538
Alw21I GWGCWC 1 cut(s) 65
AlwI GGATC 1 cut(s) 544
AoxI GGCC 2 cut(s) 25, 276
ApeKI GCWGC 4 cut(s) 91, 199, 429, 535
ApoI RAATTY 2 cut(s) 70, 394
AsiGI ACCGGT 1 cut(s) 616
AspLEI GCGC 1 cut(s) 529
AspS9I GGNCC 4 cut(s) 25, 276, 319, 328
AsuC2I CCSGG 3 cut(s) 24, 323, 465
AvaII GGWCC 2 cut(s) 319, 328
BaeGI GKGCMC 1 cut(s) 180
BanII GRGCYC 2 cut(s) 9, 65
Bbv12I GWGCWC 1 cut(s) 65
BbvI GCAGC 4 cut(s) 78, 211, 441, 547
BccI CCATC 2 cut(s) 200, 409
BcgI CGANNNNNNTGC 4 cut(s) 482, 492, 516, 526
BcnI CCSGG 3 cut(s) 24, 323, 465
BfaI CTAG 1 cut(s) 35
BisI GCNGC 5 cut(s) 92, 200, 279, 430, 536
BlpI GCTNAGC 1 cut(s) 18
BlsI GCNGC 5 cut(s) 93, 201, 280, 431, 537
BmcAI AGTACT 1 cut(s) 449
Bme1390I CCNGG 3 cut(s) 24, 323, 465
Bme18I GGWCC 2 cut(s) 319, 328
BmgT120I GGNCC 4 cut(s) 25, 276, 319, 328
BmiI GGNNCC 2 cut(s) 299, 321
BmrFI CCNGG 3 cut(s) 24, 323, 465
BmsI GCATC 1 cut(s) 58
BpmI CTGGAG 1 cut(s) 284
Bpu1102I GCTNAGC 1 cut(s) 18
BpuMI CCSGG 3 cut(s) 24, 323, 465
Bsa29I ATCGAT 1 cut(s) 502
BsaJI CCNNGG 1 cut(s) 593
BsaWI WCCGGW 1 cut(s) 616
Bsc4I CCNNNNNNNGG 2 cut(s) 285, 667
Bse118I RCCGGY 1 cut(s) 616
BseCI ATCGAT 1 cut(s) 502
BseDI CCNNGG 1 cut(s) 593
BseGI GGATG 2 cut(s) 145, 211
BseLI CCNNNNNNNGG 2 cut(s) 285, 667
BseMII CTCAG 3 cut(s) 32, 381, 444
BseSI GKGCMC 1 cut(s) 180
BseXI GCAGC 4 cut(s) 78, 211, 441, 547
BseYI CCCAGC 1 cut(s) 257
BsgI GTGCAG 1 cut(s) 424
Bsh1236I CGCG 2 cut(s) 147, 527
Bsh1285I CGRYCG 1 cut(s) 506
BshFI GGCC 2 cut(s) 27, 278
BshTI ACCGGT 1 cut(s) 616
BshVI ATCGAT 1 cut(s) 502
BsiEI CGRYCG 1 cut(s) 506
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 4 cut(s) 23, 323, 465, 617
BslI CCNNNNNNNGG 2 cut(s) 285, 667
BsnI GGCC 2 cut(s) 27, 278
Bsp1286I GDGCHC 3 cut(s) 9, 65, 180
Bsp143I GATC 4 cut(s) 142, 499, 503, 549
Bsp1720I GCTNAGC 1 cut(s) 18
Bsp68I TCGCGA 1 cut(s) 147
BspACI CCGC 6 cut(s) 89, 202, 245, 274, 279, 317
BspANI GGCC 2 cut(s) 27, 278
BspCNI CTCAG 3 cut(s) 31, 382, 445
BspDI ATCGAT 1 cut(s) 502
BspFNI CGCG 2 cut(s) 147, 527
BspLI GGNNCC 2 cut(s) 299, 321
BspPI GGATC 1 cut(s) 544
BsrFI RCCGGY 1 cut(s) 616
BssAI RCCGGY 1 cut(s) 616
BssECI CCNNGG 1 cut(s) 593
BssMI GATC 4 cut(s) 142, 499, 503, 549
Bst4CI ACNGT 1 cut(s) 127
Bst6I CTCTTC 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 276
BstDEI CTNAG 3 cut(s) 18, 390, 453
BstENI CCTNNNNNAGG 1 cut(s) 665
BstF5I GGATG 2 cut(s) 145, 211
BstFNI CGCG 2 cut(s) 147, 527
BstHHI GCGC 1 cut(s) 529
BstKTI GATC 4 cut(s) 145, 502, 506, 552
BstMBI GATC 4 cut(s) 142, 499, 503, 549
BstMCI CGRYCG 1 cut(s) 506
BstMWI GCNNNNNNNGC 4 cut(s) 97, 275, 535, 604
BstSCI CCNGG 3 cut(s) 22, 321, 463
BstSLI GKGCMC 1 cut(s) 180
BstUI CGCG 2 cut(s) 147, 527
BstV1I GCAGC 4 cut(s) 78, 211, 441, 547
BstXI CCANNNNNNTGG 1 cut(s) 560
Bsu15I ATCGAT 1 cut(s) 502
BsuRI GGCC 2 cut(s) 27, 278
BsuTUI ATCGAT 1 cut(s) 502
BtsCI GGATG 2 cut(s) 145, 211
BtuMI TCGCGA 1 cut(s) 147
Cac8I GCNNGC 1 cut(s) 276
CfoI GCGC 1 cut(s) 529
Cfr10I RCCGGY 1 cut(s) 616
Cfr13I GGNCC 4 cut(s) 25, 276, 319, 328
ClaI ATCGAT 1 cut(s) 502
Csp6I GTAC 3 cut(s) 57, 448, 590
CspAI ACCGGT 1 cut(s) 616
CviAII CATG 1 cut(s) 332
CviQI GTAC 3 cut(s) 57, 448, 590
DdeI CTNAG 3 cut(s) 18, 390, 453
DpnI GATC 4 cut(s) 144, 501, 505, 551
DpnII GATC 4 cut(s) 142, 499, 503, 549
DrdI GACNNNNNNGTC 1 cut(s) 326
DseDI GACNNNNNNGTC 1 cut(s) 326
Eam1104I CTCTTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 144
Ecl136II GAGCTC 1 cut(s) 63
Eco24I GRGCYC 2 cut(s) 9, 65
Eco47I GGWCC 2 cut(s) 319, 328
Eco53kI GAGCTC 1 cut(s) 63
EcoICRI GAGCTC 1 cut(s) 63
EcoNI CCTNNNNNAGG 1 cut(s) 665
EcoRI GAATTC 1 cut(s) 70
EcoT38I GRGCYC 2 cut(s) 9, 65
FaeI CATG 1 cut(s) 335
FaiI YATR 8 cut(s) 333, 341, 461, 495, 516, 646, 674, 676
FatI CATG 1 cut(s) 331
FauI CCCGC 2 cut(s) 195, 267
FauNDI CATATG 1 cut(s) 674
FblI GTMKAC 1 cut(s) 611
Fnu4HI GCNGC 5 cut(s) 92, 200, 279, 430, 536
FokI GGATG 2 cut(s) 152, 218
FriOI GRGCYC 2 cut(s) 9, 65
Fsp4HI GCNGC 5 cut(s) 92, 200, 279, 430, 536
FspBI CTAG 1 cut(s) 35
GlaI GCGC 1 cut(s) 528
GluI GCNGC 5 cut(s) 92, 200, 279, 430, 536
GsaI CCCAGC 1 cut(s) 261
GsuI CTGGAG 1 cut(s) 284
HaeIII GGCC 2 cut(s) 27, 278
HapII CCGG 4 cut(s) 23, 323, 465, 617
HhaI GCGC 1 cut(s) 529
Hin1II CATG 1 cut(s) 335
Hin6I GCGC 1 cut(s) 527
HinP1I GCGC 1 cut(s) 527
HinfI GANTC 2 cut(s) 251, 655
HpaII CCGG 4 cut(s) 23, 323, 465, 617
Hpy166II GTNNAC 2 cut(s) 220, 612
Hpy188I TCNGA 4 cut(s) 54, 215, 391, 454
Hpy188III TCNNGA 5 cut(s) 146, 520, 547, 553, 665
Hpy8I GTNNAC 2 cut(s) 220, 612
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 2 cut(s) 340, 671
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4V TGCA 2 cut(s) 94, 405
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 275, 535, 604
HpyF3I CTNAG 3 cut(s) 18, 390, 453
Hsp92II CATG 1 cut(s) 335
HspAI GCGC 1 cut(s) 527
Kzo9I GATC 4 cut(s) 142, 499, 503, 549
LmnI GCTCC 2 cut(s) 467, 632
Lsp1109I GCAGC 4 cut(s) 78, 211, 441, 547
LweI GCATC 1 cut(s) 58
MaeI CTAG 1 cut(s) 35
MalI GATC 4 cut(s) 144, 501, 505, 551
MboI GATC 4 cut(s) 142, 499, 503, 549
MboII GAAGA 1 cut(s) 161
MfeI CAATTG 1 cut(s) 95
MhlI GDGCHC 3 cut(s) 9, 65, 180
MluCI AATT 6 cut(s) 70, 95, 336, 377, 394, 508
MspA1I CMGCKG 3 cut(s) 91, 202, 281
MspI CCGG 4 cut(s) 23, 323, 465, 617
MspR9I CCNGG 3 cut(s) 24, 323, 465
MunI CAATTG 1 cut(s) 95
MvnI CGCG 2 cut(s) 147, 527
MwoI GCNNNNNNNGC 4 cut(s) 97, 275, 535, 604
NciI CCSGG 3 cut(s) 24, 323, 465
NdeI CATATG 1 cut(s) 674
NdeII GATC 4 cut(s) 142, 499, 503, 549
NlaIII CATG 1 cut(s) 335
NlaIV GGNNCC 2 cut(s) 299, 321
NmeAIII GCCGAG 1 cut(s) 574
NruI TCGCGA 1 cut(s) 147
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PfeI GAWTC 2 cut(s) 251, 655
PinAI ACCGGT 1 cut(s) 616
PkrI GCNGC 5 cut(s) 93, 201, 280, 431, 537
Ple19I CGATCG 1 cut(s) 506
Psp124BI GAGCTC 1 cut(s) 65
PspFI CCCAGC 1 cut(s) 257
PspN4I GGNNCC 2 cut(s) 299, 321
PspPI GGNCC 4 cut(s) 25, 276, 319, 328
PvuI CGATCG 1 cut(s) 506
RruI TCGCGA 1 cut(s) 147
RsaI GTAC 3 cut(s) 58, 449, 591
RsaNI GTAC 3 cut(s) 57, 448, 590
SacI GAGCTC 1 cut(s) 65
SatI GCNGC 5 cut(s) 92, 200, 279, 430, 536
Sau3AI GATC 4 cut(s) 142, 499, 503, 549
Sau96I GGNCC 4 cut(s) 25, 276, 319, 328
ScaI AGTACT 1 cut(s) 449
ScrFI CCNGG 3 cut(s) 24, 323, 465
SduI GDGCHC 3 cut(s) 9, 65, 180
SetI ASST 8 cut(s) 65, 171, 330, 373, 412, 424, 540, 595
SfaNI GCATC 1 cut(s) 58
SinI GGWCC 2 cut(s) 319, 328
Sse9I AATT 6 cut(s) 70, 95, 336, 377, 394, 508
SsiI CCGC 6 cut(s) 89, 202, 245, 274, 279, 317
SspMI CTAG 1 cut(s) 35
SstI GAGCTC 1 cut(s) 65
StyD4I CCNGG 3 cut(s) 22, 321, 463
TaaI ACNGT 1 cut(s) 127
TaqI TCGA 4 cut(s) 68, 102, 502, 506
TaqII GACCGA 1 cut(s) 276
TasI AATT 6 cut(s) 70, 95, 336, 377, 394, 508
TatI WGTACW 1 cut(s) 447
TauI GCSGC 1 cut(s) 281
TfiI GAWTC 2 cut(s) 251, 655
TseI GCWGC 4 cut(s) 91, 199, 429, 535
TspDTI ATGAA 1 cut(s) 348
TspGWI ACGGA 2 cut(s) 26, 73
VpaK11BI GGWCC 2 cut(s) 319, 328
XagI CCTNNNNNAGG 1 cut(s) 665
XapI RAATTY 2 cut(s) 70, 394
XmiI GTMKAC 1 cut(s) 611
XspI CTAG 1 cut(s) 35
ZrmI AGTACT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.