Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0004637

Physical Location & Seq

Genomic Coordinates Reverse (-)

66473473 .. 66478379

Gene Structure 4907 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ21553

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1710 bp

ATGGATTTCTCCGGGGCTCAGCCGGGTCTGTTTCTAGGGCTGAACTCGCATCAGAGTTCAATTCCGACGAATTGTAATCAATCCGTGCCGCTACAGCTGCTGAAGGAGGAGAATATTAGGGCTGAGAATGGAGACGAGCGCGAGGAGGAGGAGGAGGAGGAAGAAGAAGATGAGAGTGAGAGATTCAATCTCTTAGGGCACTCAATTTGCCCCAAAAGGCCGAGGGACTTGTCGTCACCCTTCAATCAGCCTCCTGCCAAGTGCGCGGCGGTTGAATCGGAGGGGCTGGAGGCGCGGCGGGCCGCGGTGAGGGCTTGGGGGAACCAGCCGCTTGCGGTGGCGGACCCGGAGATTCATGAAATGATGGAGAAGGAGAGGCGTAGGCAGTTCAAAGGTATCGAATTGATTGCTTCGGAGAATTTCGTGTGCAGGGCTGTGATGGAAGCTCTGGGCAGCCACTTGACGAATAAGTATTCGGAGGGGTTCCCCGGAGGGAGGTACTATACTGGTAATCAGCATATTGATCAGATTGAATTGCTCTGCTGTGAGAGGGCATTGGCTGCTTTTAGTCTCGATCCTGAGAAATGGGGGGTCAATGTTCAGCCTTACTCTTGTACTTCGGCTAATTTCGCTGTATACACTGGTCTTTTGCTCCCAAATGAACGGATTATGGGTTTGGATTCTCCGTCCGGAGGACACATGAGTCATGGTTATTATACTCCTAGTGGGAAGAAGGTGTCTGCCGCATCGATTTTCTTTGACAGTCTTCCTTATAAGGTGAACCCCCGCACGGGGCTCATTGATTATGATAAGCTTGAGGAGAAGGCGCTTGATTATCGCCCCAAGATACTTATTTGCGGCGGGAGTTCGTATCCCCGGGAGTGGGATTATGCCAGGTTTAGGTATATTGCTGATAAATGCGGAGCTGTGCTGATGTGTGACATGGCTCATATCAGCGGCCTTATAGCAGCCAAGGAGTGTGTTAGTCCTTTTGATTATTGTGATATTGTTACGTCAACAACACACAAAAGCCTCCGGGGTCCTAGGGGAGGTATTATATTTTACCGGAAAGGTCCAAAAAGAAGGAAGCAAGGCATGCATCAATCAAATGGGGATGGAAATAACCTGTATGATTTTGAAGAAAAGATAAATTTCGCTGTTTTCCCATCGCTACAGGGTGGTCCTCACAACAACCATATTGCTGCTCTTGCCATAGCCTTAAAACAAGTGGCTACTCCAGAATATAAAGAATACATGAACCAGGTCAAGAAAAATGCACAGTCCTTGGCAAATGCTTTGTTAAGAAGAAAATGCAGATTGGTTACTGGGGGTACCGATAATCACTTGTTGCTTTGGGATCTTACAGCTCTTGGTATAACAGGGAAGAACTACGAAAAAGTTTGCGAGATCTGCCATATTACTCTCAATAAAAGTGCCATATTTGGTGATAACGGAGCCTTTTCTCCTGGGGGAGTTAGAATTGGTACCCCTGCTATGACTTCACGAGGTTGTGTTGAGTCTGATTTCGAGACAATTGCCGACTTTCTTCTTAGGGCTGCCCAGATTACTGTTGCTGTTAAGAGAGAACATGGAAAATATCAGAAAGAATCTCTCAAAGGTCTCCAGAACAATAAAGATATTGTTGAACTTCGAAATCGTGTTGAAACATTTGCTTCCCAATTTGAAATGCCAGGATATGATACTTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004372 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005759 GO:0006730 GO:0006732 GO:0006790 GO:0008150 GO:0008152 GO:0009987 GO:0010035 GO:0010038 GO:0016740 GO:0016741 GO:0016742 GO:0017144 GO:0019219 GO:0019222 GO:0031323 GO:0031974 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044030 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046500 GO:0046686 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051052 GO:0051171 GO:0051186 GO:0055063 GO:0055081 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0072505 GO:0080090 GO:0098771

KEGG Pathways

Metabolic & Signaling

ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523

Pfam Domains

Protein Families

SHMT

Protein Analysis

569

Amino Acids

63.38

Weight (kDa)

6.24

Isoelectric Point (pI)

52.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SHMT	PF00464	112 - 515	4.4e-172	Serine hydroxymethyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004637)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22020 AT1G22020 AT1G36370
fragaria_vesca	FvH4_5g28970 FvH4_5g38450 FvH4_5g38450
malus_domestica	MD04G1132900.v1.1 MD12G1145600.v1.1
prunus_persica	Prupe.1G507500_v2.0.a1 Prupe.1G507600_v2.0.a1
pyrus_communis	pycom04g12080 pycom12g13990 pycom15g32210
rosa_chinensis	RchiOBHm_Chr7g0221291 RchiOBHm_Chr7g0240491
rosa_laevigata	RLG00000000675 RLG00000002164
rosa_multiflora	Rmu_sc0000324.1_g000020 Rmu_sc0005327.1_g000009
rosa_roxburghii	Rroxscaffold_3G00219940 Rroxscaffold_3G00238440
rosa_rugosa	Rorug07G0202000 Rorug07G0336600
rosa_samantha	Rh7AG341100 Rh7AG491000 Rh7BG332100 Rh7BG463500 Rh7CG359000 Rh7CG507100
rosa_wichuraiana	Rw7G029010 Rw7G041140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	774
AasI	GACNNNNNNGTC	1 cut(s)	702
Acc65I	GGTACC	2 cut(s)	1331, 1484
AccB1I	GGYRCC	2 cut(s)	1331, 1484
AccI	GTMKAC	1 cut(s)	636
AccII	CGCG	4 cut(s)	141, 266, 295, 305
AccIII	TCCGGA	1 cut(s)	689
AclWI	GGATC	2 cut(s)	569, 1365
AcsI	RAATTY	2 cut(s)	418, 1150
AcuI	CTGAAG	1 cut(s)	122
AfaI	GTAC	4 cut(s)	500, 616, 1333, 1486
AhdI	GACNNNNNGTC	1 cut(s)	232
AjnI	CCWGG	4 cut(s)	893, 1260, 1465, 1690
AluBI	AGCT	5 cut(s)	97, 446, 814, 926, 1367
AluI	AGCT	5 cut(s)	97, 446, 814, 926, 1367
Alw26I	GTCTC	4 cut(s)	126, 575, 1523, 1625
AlwI	GGATC	2 cut(s)	569, 1365
AlwNI	CAGNNNCTG	1 cut(s)	100
Ama87I	CYCGRG	1 cut(s)	876
Aor13HI	TCCGGA	1 cut(s)	689
AoxI	GGCC	3 cut(s)	218, 300, 958
ApeKI	GCWGC	6 cut(s)	97, 453, 560, 968, 1202, 1556
ApoI	RAATTY	2 cut(s)	418, 1150
Asp718I	GGTACC	2 cut(s)	1331, 1484
AspA2I	CCTAGG	1 cut(s)	1043
AspLEI	GCGC	4 cut(s)	141, 266, 295, 829
AspS9I	GGNCC	5 cut(s)	300, 343, 1040, 1073, 1181
AsuC2I	CCSGG	7 cut(s)	13, 24, 347, 489, 877, 878, 1037
AsuHPI	GGTGA	4 cut(s)	228, 319, 790, 1457
AsuII	TTCGAA	1 cut(s)	1651
AvaI	CYCGRG	1 cut(s)	876
AvaII	GGWCC	4 cut(s)	343, 1040, 1073, 1181
AvrII	CCTAGG	1 cut(s)	1043
BaeGI	GKGCMC	1 cut(s)	201
BanI	GGYRCC	2 cut(s)	1331, 1484
BanII	GRGCYC	2 cut(s)	19, 798
BauI	CACGAG	1 cut(s)	1503
BbsI	GAAGAC	1 cut(s)	758
BbvI	GCAGC	6 cut(s)	84, 465, 547, 980, 1189, 1543
BccI	CCATC	4 cut(s)	358, 433, 1109, 1174
BcgI	CGANNNNNNTGC	4 cut(s)	389, 423, 1517, 1551
BciT130I	CCWGG	4 cut(s)	895, 1262, 1467, 1692
BciVI	GTATCC	1 cut(s)	882
BclI	TGATCA	1 cut(s)	523
BcnI	CCSGG	7 cut(s)	13, 24, 347, 489, 877, 878, 1037
BcoDI	GTCTC	4 cut(s)	126, 575, 1523, 1625
BfaI	CTAG	3 cut(s)	35, 723, 1044
BfmI	CTRYAG	2 cut(s)	92, 1172
BfoI	RGCGCY	1 cut(s)	830
BfuI	GTATCC	1 cut(s)	882
BglII	AGATCT	1 cut(s)	1407
BlnI	CCTAGG	1 cut(s)	1043
BlpI	GCTNAGC	1 cut(s)	18
Bme18I	GGWCC	4 cut(s)	343, 1040, 1073, 1181
BmeRI	GACNNNNNGTC	1 cut(s)	232
BmeT110I	CYCGRG	1 cut(s)	876
BmgT120I	GGNCC	5 cut(s)	300, 343, 1040, 1073, 1181
BmiI	GGNNCC	7 cut(s)	323, 345, 485, 1041, 1333, 1456, 1486
BmrI	ACTGGG	1 cut(s)	1335
BmsI	GCATC	3 cut(s)	58, 755, 1108
BmuI	ACTGGG	1 cut(s)	1335
BpiI	GAAGAC	1 cut(s)	758
BpmI	CTGGAG	3 cut(s)	308, 1221, 1607
Bpu1102I	GCTNAGC	1 cut(s)	18
Bpu14I	TTCGAA	1 cut(s)	1651
BpuEI	CTTGAG	1 cut(s)	836
BpuMI	CCSGG	7 cut(s)	13, 24, 347, 489, 877, 878, 1037
Bsa29I	ATCGAT	1 cut(s)	749
BsaI	GGTCTC	1 cut(s)	1625
BsaWI	WCCGGW	2 cut(s)	689, 1065
Bse1I	ACTGG	3 cut(s)	511, 646, 1330
BseAI	TCCGGA	1 cut(s)	689
BseBI	CCWGG	4 cut(s)	895, 1262, 1467, 1692
BseCI	ATCGAT	1 cut(s)	749
BseGI	GGATG	1 cut(s)	1120
BseMII	CTCAG	3 cut(s)	32, 114, 570
BseNI	ACTGG	3 cut(s)	511, 646, 1330
BseRI	GAGGAG	7 cut(s)	122, 158, 161, 164, 167, 170, 833
BseSI	GKGCMC	1 cut(s)	201
BseXI	GCAGC	6 cut(s)	84, 465, 547, 980, 1189, 1543
BsgI	GTGCAG	1 cut(s)	448
Bsh1236I	CGCG	4 cut(s)	141, 266, 295, 305
BshFI	GGCC	3 cut(s)	220, 302, 960
BshNI	GGYRCC	2 cut(s)	1331, 1484
BshVI	ATCGAT	1 cut(s)	749
BsiHKCI	CYCGRG	1 cut(s)	876
BsiSI	CCGG	8 cut(s)	12, 23, 347, 489, 690, 877, 1036, 1066
BslFI	GGGAC	1 cut(s)	239
BsmAI	GTCTC	4 cut(s)	126, 575, 1523, 1625
BsmBI	CGTCTC	1 cut(s)	126
BsmFI	GGGAC	1 cut(s)	239
BsnI	GGCC	3 cut(s)	220, 302, 960
Bso31I	GGTCTC	1 cut(s)	1625
BsoBI	CYCGRG	1 cut(s)	876
Bsp119I	TTCGAA	1 cut(s)	1651
Bsp1286I	GDGCHC	3 cut(s)	19, 201, 798
Bsp13I	TCCGGA	1 cut(s)	689
Bsp143I	GATC	4 cut(s)	523, 574, 1357, 1407
Bsp1720I	GCTNAGC	1 cut(s)	18
BspANI	GGCC	3 cut(s)	220, 302, 960
BspCNI	CTCAG	3 cut(s)	31, 115, 571
BspDI	ATCGAT	1 cut(s)	749
BspEI	TCCGGA	1 cut(s)	689
BspFNI	CGCG	4 cut(s)	141, 266, 295, 305
BspHI	TCATGA	1 cut(s)	355
BspLI	GGNNCC	7 cut(s)	323, 345, 485, 1041, 1333, 1456, 1486
BspPI	GGATC	2 cut(s)	569, 1365
BspT104I	TTCGAA	1 cut(s)	1651
BspT107I	GGYRCC	2 cut(s)	1331, 1484
BspTNI	GGTCTC	1 cut(s)	1625
BsrI	ACTGG	3 cut(s)	511, 646, 1330
BssMI	GATC	4 cut(s)	523, 574, 1357, 1407
BssNAI	GTATAC	1 cut(s)	637
BssSI	CACGAG	1 cut(s)	1503
BssT1I	CCWWGG	3 cut(s)	972, 1043, 1284
Bst1107I	GTATAC	1 cut(s)	637
Bst2BI	CACGAG	1 cut(s)	1503
Bst2UI	CCWGG	4 cut(s)	895, 1262, 1467, 1692
Bst4CI	ACNGT	3 cut(s)	764, 1281, 1570
BstAPI	GCANNNNNTGC	2 cut(s)	560, 1096
BstBI	TTCGAA	1 cut(s)	1651
BstC8I	GCNNGC	3 cut(s)	300, 333, 1097
BstDEI	CTNAG	5 cut(s)	18, 123, 193, 579, 1550
BstDSI	CCRYGG	1 cut(s)	303
BstENI	CCTNNNNNAGG	1 cut(s)	1047
BstF5I	GGATG	1 cut(s)	1120
BstFNI	CGCG	4 cut(s)	141, 266, 295, 305
BstH2I	RGCGCY	1 cut(s)	830
BstHHI	GCGC	4 cut(s)	141, 266, 295, 829
BstKTI	GATC	4 cut(s)	526, 577, 1360, 1410
BstMAI	GTCTC	4 cut(s)	126, 575, 1523, 1625
BstMBI	GATC	4 cut(s)	523, 574, 1357, 1407
BstNI	CCWGG	4 cut(s)	895, 1262, 1467, 1692
BstNSI	RCATGY	1 cut(s)	1099
BstSFI	CTRYAG	2 cut(s)	92, 1172
BstSLI	GKGCMC	1 cut(s)	201
BstUI	CGCG	4 cut(s)	141, 266, 295, 305
BstV1I	GCAGC	6 cut(s)	84, 465, 547, 980, 1189, 1543
BstV2I	GAAGAC	1 cut(s)	758
BstX2I	RGATCY	2 cut(s)	1357, 1407
BstYI	RGATCY	2 cut(s)	1357, 1407
BstZ17I	GTATAC	1 cut(s)	637
Bsu15I	ATCGAT	1 cut(s)	749
BsuI	GTATCC	1 cut(s)	882
BsuRI	GGCC	3 cut(s)	220, 302, 960
BsuTUI	ATCGAT	1 cut(s)	749
BtgI	CCRYGG	1 cut(s)	303
BtgZI	GCGATG	1 cut(s)	1152
BtsCI	GGATG	1 cut(s)	1120
BtsIMutI	CAGTG	1 cut(s)	639
Cac8I	GCNNGC	3 cut(s)	300, 333, 1097
CaiI	CAGNNNCTG	1 cut(s)	100
CciI	TCATGA	1 cut(s)	355
CfoI	GCGC	4 cut(s)	141, 266, 295, 829
Cfr13I	GGNCC	5 cut(s)	300, 343, 1040, 1073, 1181
Cfr42I	CCGCGG	1 cut(s)	306
Cfr9I	CCCGGG	1 cut(s)	876
ClaI	ATCGAT	1 cut(s)	749
CsiI	ACCWGGT	1 cut(s)	1260
Csp6I	GTAC	4 cut(s)	499, 615, 1332, 1485
CviAII	CATG	7 cut(s)	356, 700, 707, 943, 1096, 1255, 1589
CviQI	GTAC	4 cut(s)	499, 615, 1332, 1485
DdeI	CTNAG	5 cut(s)	18, 123, 193, 579, 1550
DpnI	GATC	4 cut(s)	525, 576, 1359, 1409
DpnII	GATC	4 cut(s)	523, 574, 1357, 1407
DrdI	GACNNNNNNGTC	1 cut(s)	702
DriI	GACNNNNNGTC	1 cut(s)	232
DseDI	GACNNNNNNGTC	1 cut(s)	702
Eam1105I	GACNNNNNGTC	1 cut(s)	232
EciI	GGCGGA	1 cut(s)	356
Eco130I	CCWWGG	3 cut(s)	972, 1043, 1284
Eco24I	GRGCYC	2 cut(s)	19, 798
Eco31I	GGTCTC	1 cut(s)	1625
Eco47I	GGWCC	4 cut(s)	343, 1040, 1073, 1181
Eco57I	CTGAAG	1 cut(s)	122
Eco88I	CYCGRG	1 cut(s)	876
EcoNI	CCTNNNNNAGG	1 cut(s)	1047
EcoO109I	RGGNCCY	1 cut(s)	1040
EcoRII	CCWGG	4 cut(s)	893, 1260, 1465, 1690
EcoT14I	CCWWGG	3 cut(s)	972, 1043, 1284
EcoT22I	ATGCAT	1 cut(s)	1101
EcoT38I	GRGCYC	2 cut(s)	19, 798
ErhI	CCWWGG	3 cut(s)	972, 1043, 1284
Esp3I	CGTCTC	1 cut(s)	126
FaeI	CATG	7 cut(s)	359, 703, 710, 946, 1099, 1258, 1592
FaqI	GGGAC	1 cut(s)	239
FatI	CATG	7 cut(s)	355, 699, 706, 942, 1095, 1254, 1588
FauI	CCCGC	3 cut(s)	291, 794, 854
FbaI	TGATCA	1 cut(s)	523
FblI	GTMKAC	1 cut(s)	636
FokI	GGATG	1 cut(s)	1127
FriOI	GRGCYC	2 cut(s)	19, 798
FspBI	CTAG	3 cut(s)	35, 723, 1044
GlaI	GCGC	4 cut(s)	140, 265, 294, 828
GsuI	CTGGAG	3 cut(s)	308, 1221, 1607
HaeII	RGCGCY	1 cut(s)	830
HaeIII	GGCC	3 cut(s)	220, 302, 960
HapII	CCGG	8 cut(s)	12, 23, 347, 489, 690, 877, 1036, 1066
HhaI	GCGC	4 cut(s)	141, 266, 295, 829
Hin1II	CATG	7 cut(s)	359, 703, 710, 946, 1099, 1258, 1592
Hin6I	GCGC	4 cut(s)	139, 264, 293, 827
HinP1I	GCGC	4 cut(s)	139, 264, 293, 827
HincII	GTYRAC	1 cut(s)	1017
HindII	GTYRAC	1 cut(s)	1017
HindIII	AAGCTT	1 cut(s)	812
HinfI	GANTC	7 cut(s)	183, 275, 352, 680, 703, 1517, 1607
HpaII	CCGG	8 cut(s)	12, 23, 347, 489, 690, 877, 1036, 1066
HphI	GGTGA	4 cut(s)	228, 319, 790, 1457
Hpy166II	GTNNAC	3 cut(s)	637, 781, 1017
Hpy188I	TCNGA	8 cut(s)	54, 66, 280, 415, 478, 528, 1522, 1602
Hpy188III	TCNNGA	9 cut(s)	356, 572, 578, 690, 1238, 1267, 1503, 1528, 1624
Hpy8I	GTNNAC	3 cut(s)	637, 781, 1017
Hpy99I	CGWCG	1 cut(s)	70
HpyAV	CCTTC	6 cut(s)	97, 250, 364, 727, 817, 1077
HpyCH4III	ACNGT	3 cut(s)	764, 1281, 1570
HpyCH4IV	ACGT	1 cut(s)	1013
HpyCH4V	TGCA	4 cut(s)	429, 1099, 1277, 1314
HpyF3I	CTNAG	5 cut(s)	18, 123, 193, 579, 1550
HpySE526I	ACGT	1 cut(s)	1013
Hsp92II	CATG	7 cut(s)	359, 703, 710, 946, 1099, 1258, 1592
HspAI	GCGC	4 cut(s)	139, 264, 293, 827
Kpn2I	TCCGGA	1 cut(s)	689
KpnI	GGTACC	2 cut(s)	1335, 1488
Ksp22I	TGATCA	1 cut(s)	523
KspI	CCGCGG	1 cut(s)	306
Kzo9I	GATC	4 cut(s)	523, 574, 1357, 1407
LmnI	GCTCC	3 cut(s)	657, 923, 1454
Lsp1109I	GCAGC	6 cut(s)	84, 465, 547, 980, 1189, 1543
LweI	GCATC	3 cut(s)	58, 755, 1108
MabI	ACCWGGT	1 cut(s)	1260
MaeI	CTAG	3 cut(s)	35, 723, 1044
MaeII	ACGT	1 cut(s)	1013
MaeIII	GTNAC	4 cut(s)	234, 938, 1009, 1321
MalI	GATC	4 cut(s)	525, 576, 1359, 1409
MboI	GATC	4 cut(s)	523, 574, 1357, 1407
MboII	GAAGA	9 cut(s)	173, 176, 179, 742, 758, 1151, 1317, 1396, 1538
MfeI	CAATTG	1 cut(s)	1533
MflI	RGATCY	2 cut(s)	1357, 1407
MhlI	GDGCHC	3 cut(s)	19, 201, 798
MlyI	GAGTC	2 cut(s)	712, 1526
MmeI	TCCRAC	1 cut(s)	89
Mph1103I	ATGCAT	1 cut(s)	1101
MroI	TCCGGA	1 cut(s)	689
MseI	TTAA	3 cut(s)	1220, 1301, 1578
MspA1I	CMGCKG	3 cut(s)	97, 305, 957
MspI	CCGG	8 cut(s)	12, 23, 347, 489, 690, 877, 1036, 1066
MunI	CAATTG	1 cut(s)	1533
MvaI	CCWGG	4 cut(s)	895, 1262, 1467, 1692
MvnI	CGCG	4 cut(s)	141, 266, 295, 305
NciI	CCSGG	7 cut(s)	13, 24, 347, 489, 877, 878, 1037
NdeII	GATC	4 cut(s)	523, 574, 1357, 1407
NlaIII	CATG	7 cut(s)	359, 703, 710, 946, 1099, 1258, 1592
NlaIV	GGNNCC	7 cut(s)	323, 345, 485, 1041, 1333, 1456, 1486
NmeAIII	GCCGAG	1 cut(s)	246
NmuCI	GTSAC	2 cut(s)	234, 938
NsiI	ATGCAT	1 cut(s)	1101
NspI	RCATGY	1 cut(s)	1099
NspV	TTCGAA	1 cut(s)	1651
PaeI	GCATGC	1 cut(s)	1099
PagI	TCATGA	1 cut(s)	355
PfeI	GAWTC	5 cut(s)	183, 275, 352, 680, 1607
PleI	GAGTC	2 cut(s)	711, 1525
PpsI	GAGTC	2 cut(s)	711, 1525
PpuMI	RGGWCCY	1 cut(s)	1040
PsiI	TTATAA	1 cut(s)	774
Psp5II	RGGWCCY	1 cut(s)	1040
Psp6I	CCWGG	4 cut(s)	893, 1260, 1465, 1690
PspGI	CCWGG	4 cut(s)	893, 1260, 1465, 1690
PspN4I	GGNNCC	7 cut(s)	323, 345, 485, 1041, 1333, 1456, 1486
PspPI	GGNCC	5 cut(s)	300, 343, 1040, 1073, 1181
PspPPI	RGGWCCY	1 cut(s)	1040
PstNI	CAGNNNCTG	1 cut(s)	100
PsuI	RGATCY	2 cut(s)	1357, 1407
PvuII	CAGCTG	1 cut(s)	97
RsaI	GTAC	4 cut(s)	500, 616, 1333, 1486
RsaNI	GTAC	4 cut(s)	499, 615, 1332, 1485
SacII	CCGCGG	1 cut(s)	306
SaqAI	TTAA	3 cut(s)	1220, 1301, 1578
Sau3AI	GATC	4 cut(s)	523, 574, 1357, 1407
Sau96I	GGNCC	5 cut(s)	300, 343, 1040, 1073, 1181
SchI	GAGTC	2 cut(s)	712, 1526
SduI	GDGCHC	3 cut(s)	19, 201, 798
SexAI	ACCWGGT	1 cut(s)	1260
SfaNI	GCATC	3 cut(s)	58, 755, 1108
SfcI	CTRYAG	2 cut(s)	92, 1172
Sfr303I	CCGCGG	1 cut(s)	306
SfuI	TTCGAA	1 cut(s)	1651
SgrBI	CCGCGG	1 cut(s)	306
SinI	GGWCC	4 cut(s)	343, 1040, 1073, 1181
SmaI	CCCGGG	1 cut(s)	878
SmlI	CTYRAG	1 cut(s)	815
SmoI	CTYRAG	1 cut(s)	815
SphI	GCATGC	1 cut(s)	1099
SspI	AATATT	1 cut(s)	115
SspMI	CTAG	3 cut(s)	35, 723, 1044
StyI	CCWWGG	3 cut(s)	972, 1043, 1284
TaaI	ACNGT	3 cut(s)	764, 1281, 1570
TaiI	ACGT	1 cut(s)	1016
TaqI	TCGA	5 cut(s)	399, 573, 749, 1527, 1651
TatI	WGTACW	1 cut(s)	614
TauI	GCSGC	8 cut(s)	91, 269, 298, 305, 331, 746, 861, 960
TfiI	GAWTC	5 cut(s)	183, 275, 352, 680, 1607
Tru1I	TTAA	3 cut(s)	1220, 1301, 1578
Tru9I	TTAA	3 cut(s)	1220, 1301, 1578
TscAI	CASTG	1 cut(s)	646
TseFI	GTSAC	2 cut(s)	234, 938
TseI	GCWGC	6 cut(s)	97, 453, 560, 968, 1202, 1556
Tsp45I	GTSAC	2 cut(s)	234, 938
TspDTI	ATGAA	4 cut(s)	344, 372, 675, 1271
TspGWI	ACGGA	4 cut(s)	73, 675, 679, 1467
TspMI	CCCGGG	1 cut(s)	876
TspRI	CASTG	1 cut(s)	646
VpaK11BI	GGWCC	4 cut(s)	343, 1040, 1073, 1181
XagI	CCTNNNNNAGG	1 cut(s)	1047
XapI	RAATTY	2 cut(s)	418, 1150
XceI	RCATGY	1 cut(s)	1099
XmaI	CCCGGG	1 cut(s)	876
XmaJI	CCTAGG	1 cut(s)	1043
XmiI	GTMKAC	1 cut(s)	636
XspI	CTAG	3 cut(s)	35, 723, 1044
Zsp2I	ATGCAT	1 cut(s)	1101

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.