Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0004637

Physical Location & Seq

Genomic Coordinates Forward (+)

3622978 .. 3627209

Gene Structure 4232 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000000675

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1713 bp

ATGGATTTCTCCGGGGCTCAGCCGGGTCTGTTTCTAGGGCTCAACTCGCATCAGAGTTCAATTCCGACGAATTGTAATCAATCCGTGCCGCTACAGCTGCTGAAGGAGGAGAATATTAGGGCTGAGAATGGAGACGATCGCGAGGAGGAGGAGGAGGAGGAGGAAGAAGAAGATGAGAGTGAGAGATTCAATCTTTTAGGGCACTCAATTTGCCCCAAAAGGCCGAGGGACCTGTCATCTCCTTTCAATCAGCCTCCTGCCAAGTGCGTGGTGGTGGAATCGGAAGGGCTGGAGGCGCGGCGGGCCGCGGTGAGGGCTTGGGGGAACCAGCCGCTTGCGGTGGCGGACCCGGAGATTCATGAAATGATGGAGAAGGAGAGGCGGAGGCAGTTCAAAGGTATCGAATTGATTGCTTCGGAGAATTTCGTGTGTAGGGCTGTGATGGAAGCTCTGGGCAGCCACTTGACGAATAAGTATTCGGAGGGTTTCCCCGGAGGGAGGTACTATACTGGTAATCAGCATATTGATCAGATTGAATTGCTCTGCTGTGAAAGGGCATTGGCTGCTTTTAGTCTTGATCCTGAGAAATGGGGTGTCAATGTTCAGCCTTACTCTTGTACTTCAGCTAATTTCGCTGTATACACTGGTCTTTTGCTCCCAAATGAACGGATTATGGGTTTGGATTCGCCGTCCGGAGGGCATATGAGTCATGGTTATTATACTCCTAGTGGGAAGAAGGTGTCTGCCGCATCAATATTCTTTGATAGTCTTCCTTATAAGGTGAACCCCCGCACGGGGCTCATTGATTATGATAAGCTTGAGGAGAAGGCGCTTGATTATCGCCCCAAGATTCTTATTTGCGGCGGGAGTTCGTATCCCCGGGAGTGGGATTATGCCAGGTTTAGGTATATTGCTGATAAATGCGGAGCTGTGCTGATGTGTGACATGGCTCATATCAGCGGCCTTATAGCAGCCAAAGAGTGTGCGAGTCCTTTTGATTATTGTGATATTGTTACGTCAACAACACACAAAAGCCTCCGGGGTCCTAGGGGAGGTATTATATTTTACCGGAAAGGTCCAAAAAGAAGGAAGCAAGGCATGCATCAATCAAATGGGGATGGAAATAACCTGTATGATTTTGAAGAAAAGATCAATTTCGCTGTTTTCCCATCGCTACAGGGTGGTCCTCACAACAACCATATTGCTGCTCTTGCCATAGCCTTAAAACAAGTGGCTACTCCAGAATATAAAGAATACATGAACCAGGTCAAGAAAAATGCACAGTCCTTGGCAAATGCTTTGTTAAGAAGAAAATGCAGATTGGTTACTGGGGGTACTGATAATCACTTGTTGCTTTGGGATCTTACAGCTCTTGGTATAACAGGGAAGAACTACGAAAAAGTTTGCGAGATCTGCCATATTACTCTAAATAAAAGTGCCATATTTGGTGATAACGGAGCCTTTTCCCCTGGGGGAGTTAGAATTGGTACCCCTGCTATGACTTCACGAGGTTGTGTGGAGTCTGATTTTGAGACAATTGCCGACTTTCTTCTTAGGGCTGCCCAGATTACTGTTGCTGTTCAGAGAGAACATGGAAAATATCAGAAAGAATTTCTCAAAGGTCTCCAGAACAATAAAGATATTGTTGAACTTCGAAATCGTGTTGAAACATTTGCTTCCCAATTTGAAATGCCTGGATACGATACTTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004372 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005759 GO:0006730 GO:0006732 GO:0006790 GO:0008150 GO:0008152 GO:0009987 GO:0010035 GO:0010038 GO:0016740 GO:0016741 GO:0016742 GO:0017144 GO:0019219 GO:0019222 GO:0031323 GO:0031974 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044030 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046500 GO:0046686 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051052 GO:0051171 GO:0051186 GO:0055063 GO:0055081 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0072505 GO:0080090 GO:0098771

KEGG Pathways

Metabolic & Signaling

ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523

Pfam Domains

Protein Families

SHMT

Protein Analysis

571

Amino Acids

63.58

Weight (kDa)

6.07

Isoelectric Point (pI)

52.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SHMT	PF00464	113 - 516	1.8e-172	Serine hydroxymethyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004637)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22020 AT1G22020 AT1G36370
fragaria_vesca	FvH4_5g28970 FvH4_5g38450 FvH4_5g38450
malus_domestica	MD04G1132900.v1.1 MD12G1145600.v1.1
prunus_persica	Prupe.1G507500_v2.0.a1 Prupe.1G507600_v2.0.a1
pyrus_communis	pycom04g12080 pycom12g13990 pycom15g32210
rosa_chinensis	RchiOBHm_Chr7g0221291 RchiOBHm_Chr7g0240491
rosa_laevigata	RLG00000000675 RLG00000002164
rosa_multiflora	Rmu_sc0000324.1_g000020 Rmu_sc0005327.1_g000009
rosa_roxburghii	Rroxscaffold_3G00219940 Rroxscaffold_3G00238440
rosa_rugosa	Rorug07G0202000 Rorug07G0336600
rosa_samantha	Rh7AG341100 Rh7AG491000 Rh7BG332100 Rh7BG463500 Rh7CG359000 Rh7CG507100
rosa_wichuraiana	Rw7G029010 Rw7G041140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	777
Acc65I	GGTACC	1 cut(s)	1487
AccB1I	GGYRCC	1 cut(s)	1487
AccI	GTMKAC	1 cut(s)	639
AccII	CGCG	3 cut(s)	141, 298, 308
AccIII	TCCGGA	1 cut(s)	692
AclWI	GGATC	2 cut(s)	572, 1368
AcsI	RAATTY	2 cut(s)	421, 1610
AcuI	CTGAAG	2 cut(s)	122, 606
AfaI	GTAC	4 cut(s)	503, 619, 1336, 1489
AgsI	TTSAA	9 cut(s)	60, 190, 247, 394, 536, 1142, 1649, 1667, 1688
AjnI	CCWGG	4 cut(s)	896, 1263, 1468, 1693
AluBI	AGCT	6 cut(s)	97, 449, 626, 817, 929, 1370
AluI	AGCT	6 cut(s)	97, 449, 626, 817, 929, 1370
Alw26I	GTCTC	3 cut(s)	126, 1526, 1628
AlwI	GGATC	2 cut(s)	572, 1368
AlwNI	CAGNNNCTG	1 cut(s)	100
Ama87I	CYCGRG	1 cut(s)	879
Aor13HI	TCCGGA	1 cut(s)	692
AoxI	GGCC	3 cut(s)	221, 303, 961
ApeKI	GCWGC	6 cut(s)	97, 456, 563, 971, 1205, 1559
ApoI	RAATTY	2 cut(s)	421, 1610
Asp718I	GGTACC	1 cut(s)	1487
AspA2I	CCTAGG	1 cut(s)	1046
AspLEI	GCGC	2 cut(s)	298, 832
AspS9I	GGNCC	6 cut(s)	229, 303, 346, 1043, 1076, 1184
AsuC2I	CCSGG	7 cut(s)	13, 24, 350, 492, 880, 881, 1040
AsuHPI	GGTGA	3 cut(s)	322, 793, 1460
AsuII	TTCGAA	1 cut(s)	1654
AvaI	CYCGRG	1 cut(s)	879
AvaII	GGWCC	5 cut(s)	229, 346, 1043, 1076, 1184
AvrII	CCTAGG	1 cut(s)	1046
BaeGI	GKGCMC	1 cut(s)	204
BanI	GGYRCC	1 cut(s)	1487
BanII	GRGCYC	3 cut(s)	19, 42, 801
BauI	CACGAG	1 cut(s)	1506
BbsI	GAAGAC	1 cut(s)	761
BbvI	GCAGC	6 cut(s)	84, 468, 550, 983, 1192, 1546
BccI	CCATC	4 cut(s)	361, 436, 1112, 1177
BceAI	ACGGC	1 cut(s)	673
BcgI	CGANNNNNNTGC	2 cut(s)	392, 426
BciT130I	CCWGG	4 cut(s)	898, 1265, 1470, 1695
BciVI	GTATCC	2 cut(s)	885, 1691
BclI	TGATCA	1 cut(s)	526
BcnI	CCSGG	7 cut(s)	13, 24, 350, 492, 880, 881, 1040
BcoDI	GTCTC	3 cut(s)	126, 1526, 1628
BfaI	CTAG	3 cut(s)	35, 726, 1047
BfmI	CTRYAG	2 cut(s)	92, 1175
BfoI	RGCGCY	1 cut(s)	833
BfuI	GTATCC	2 cut(s)	885, 1691
BglII	AGATCT	1 cut(s)	1410
BlnI	CCTAGG	1 cut(s)	1046
BlpI	GCTNAGC	1 cut(s)	18
Bme18I	GGWCC	5 cut(s)	229, 346, 1043, 1076, 1184
BmeT110I	CYCGRG	1 cut(s)	879
BmgT120I	GGNCC	6 cut(s)	229, 303, 346, 1043, 1076, 1184
BmiI	GGNNCC	6 cut(s)	230, 326, 348, 1044, 1459, 1489
BmrI	ACTGGG	1 cut(s)	1338
BmsI	GCATC	3 cut(s)	58, 758, 1111
BmuI	ACTGGG	1 cut(s)	1338
BpiI	GAAGAC	1 cut(s)	761
BpmI	CTGGAG	3 cut(s)	311, 1224, 1610
Bpu1102I	GCTNAGC	1 cut(s)	18
Bpu14I	TTCGAA	1 cut(s)	1654
BpuEI	CTTGAG	1 cut(s)	839
BpuMI	CCSGG	7 cut(s)	13, 24, 350, 492, 880, 881, 1040
BsaI	GGTCTC	1 cut(s)	1628
BsaWI	WCCGGW	2 cut(s)	692, 1068
Bse1I	ACTGG	3 cut(s)	514, 649, 1333
BseAI	TCCGGA	1 cut(s)	692
BseBI	CCWGG	4 cut(s)	898, 1265, 1470, 1695
BseGI	GGATG	1 cut(s)	1123
BseMII	CTCAG	3 cut(s)	32, 114, 573
BseNI	ACTGG	3 cut(s)	514, 649, 1333
BseRI	GAGGAG	8 cut(s)	122, 158, 161, 164, 167, 170, 173, 836
BseSI	GKGCMC	1 cut(s)	204
BseXI	GCAGC	6 cut(s)	84, 468, 550, 983, 1192, 1546
Bsh1236I	CGCG	3 cut(s)	141, 298, 308
Bsh1285I	CGRYCG	1 cut(s)	139
BshFI	GGCC	3 cut(s)	223, 305, 963
BshNI	GGYRCC	1 cut(s)	1487
BsiEI	CGRYCG	1 cut(s)	139
BsiHKCI	CYCGRG	1 cut(s)	879
BsiSI	CCGG	8 cut(s)	12, 23, 350, 492, 693, 880, 1039, 1069
BslFI	GGGAC	1 cut(s)	242
BsmAI	GTCTC	3 cut(s)	126, 1526, 1628
BsmBI	CGTCTC	1 cut(s)	126
BsmFI	GGGAC	1 cut(s)	242
BsnI	GGCC	3 cut(s)	223, 305, 963
Bso31I	GGTCTC	1 cut(s)	1628
BsoBI	CYCGRG	1 cut(s)	879
Bsp119I	TTCGAA	1 cut(s)	1654
Bsp1286I	GDGCHC	4 cut(s)	19, 42, 204, 801
Bsp13I	TCCGGA	1 cut(s)	692
Bsp143I	GATC	6 cut(s)	136, 526, 577, 1149, 1360, 1410
Bsp1720I	GCTNAGC	1 cut(s)	18
Bsp68I	TCGCGA	1 cut(s)	141
BspANI	GGCC	3 cut(s)	223, 305, 963
BspCNI	CTCAG	3 cut(s)	31, 115, 574
BspEI	TCCGGA	1 cut(s)	692
BspFNI	CGCG	3 cut(s)	141, 298, 308
BspHI	TCATGA	1 cut(s)	358
BspLI	GGNNCC	6 cut(s)	230, 326, 348, 1044, 1459, 1489
BspPI	GGATC	2 cut(s)	572, 1368
BspT104I	TTCGAA	1 cut(s)	1654
BspT107I	GGYRCC	1 cut(s)	1487
BspTNI	GGTCTC	1 cut(s)	1628
BsrI	ACTGG	3 cut(s)	514, 649, 1333
BssMI	GATC	6 cut(s)	136, 526, 577, 1149, 1360, 1410
BssNAI	GTATAC	1 cut(s)	640
BssSI	CACGAG	1 cut(s)	1506
BssT1I	CCWWGG	2 cut(s)	1046, 1287
Bst1107I	GTATAC	1 cut(s)	640
Bst2BI	CACGAG	1 cut(s)	1506
Bst2UI	CCWGG	4 cut(s)	898, 1265, 1470, 1695
Bst4CI	ACNGT	2 cut(s)	1284, 1573
BstAPI	GCANNNNNTGC	2 cut(s)	563, 1099
BstBI	TTCGAA	1 cut(s)	1654
BstC8I	GCNNGC	3 cut(s)	303, 336, 1100
BstDEI	CTNAG	4 cut(s)	18, 123, 582, 1553
BstDSI	CCRYGG	1 cut(s)	306
BstENI	CCTNNNNNAGG	1 cut(s)	1050
BstF5I	GGATG	1 cut(s)	1123
BstFNI	CGCG	3 cut(s)	141, 298, 308
BstH2I	RGCGCY	1 cut(s)	833
BstHHI	GCGC	2 cut(s)	298, 832
BstKTI	GATC	6 cut(s)	139, 529, 580, 1152, 1363, 1413
BstMAI	GTCTC	3 cut(s)	126, 1526, 1628
BstMBI	GATC	6 cut(s)	136, 526, 577, 1149, 1360, 1410
BstMCI	CGRYCG	1 cut(s)	139
BstNI	CCWGG	4 cut(s)	898, 1265, 1470, 1695
BstNSI	RCATGY	1 cut(s)	1102
BstSFI	CTRYAG	2 cut(s)	92, 1175
BstSLI	GKGCMC	1 cut(s)	204
BstUI	CGCG	3 cut(s)	141, 298, 308
BstV1I	GCAGC	6 cut(s)	84, 468, 550, 983, 1192, 1546
BstV2I	GAAGAC	1 cut(s)	761
BstX2I	RGATCY	2 cut(s)	1360, 1410
BstXI	CCANNNNNNTGG	1 cut(s)	268
BstYI	RGATCY	2 cut(s)	1360, 1410
BstZ17I	GTATAC	1 cut(s)	640
BsuI	GTATCC	2 cut(s)	885, 1691
BsuRI	GGCC	3 cut(s)	223, 305, 963
BtgI	CCRYGG	1 cut(s)	306
BtgZI	GCGATG	1 cut(s)	1155
BtsCI	GGATG	1 cut(s)	1123
BtsIMutI	CAGTG	1 cut(s)	642
BtuMI	TCGCGA	1 cut(s)	141
Cac8I	GCNNGC	3 cut(s)	303, 336, 1100
CaiI	CAGNNNCTG	1 cut(s)	100
CciI	TCATGA	1 cut(s)	358
CfoI	GCGC	2 cut(s)	298, 832
Cfr13I	GGNCC	6 cut(s)	229, 303, 346, 1043, 1076, 1184
Cfr42I	CCGCGG	1 cut(s)	309
Cfr9I	CCCGGG	1 cut(s)	879
CsiI	ACCWGGT	1 cut(s)	1263
Csp6I	GTAC	4 cut(s)	502, 618, 1335, 1488
CviAII	CATG	6 cut(s)	359, 710, 946, 1099, 1258, 1592
CviQI	GTAC	4 cut(s)	502, 618, 1335, 1488
DdeI	CTNAG	4 cut(s)	18, 123, 582, 1553
DpnI	GATC	6 cut(s)	138, 528, 579, 1151, 1362, 1412
DpnII	GATC	6 cut(s)	136, 526, 577, 1149, 1360, 1410
EciI	GGCGGA	2 cut(s)	359, 397
Eco130I	CCWWGG	2 cut(s)	1046, 1287
Eco24I	GRGCYC	3 cut(s)	19, 42, 801
Eco31I	GGTCTC	1 cut(s)	1628
Eco47I	GGWCC	5 cut(s)	229, 346, 1043, 1076, 1184
Eco57I	CTGAAG	2 cut(s)	122, 606
Eco88I	CYCGRG	1 cut(s)	879
EcoNI	CCTNNNNNAGG	1 cut(s)	1050
EcoO109I	RGGNCCY	2 cut(s)	229, 1043
EcoRII	CCWGG	4 cut(s)	896, 1263, 1468, 1693
EcoT14I	CCWWGG	2 cut(s)	1046, 1287
EcoT22I	ATGCAT	1 cut(s)	1104
EcoT38I	GRGCYC	3 cut(s)	19, 42, 801
ErhI	CCWWGG	2 cut(s)	1046, 1287
Esp3I	CGTCTC	1 cut(s)	126
FaeI	CATG	6 cut(s)	362, 713, 949, 1102, 1261, 1595
FaqI	GGGAC	1 cut(s)	242
FatI	CATG	6 cut(s)	358, 709, 945, 1098, 1257, 1591
FauI	CCCGC	3 cut(s)	294, 797, 857
FauNDI	CATATG	1 cut(s)	702
FbaI	TGATCA	1 cut(s)	526
FblI	GTMKAC	1 cut(s)	639
FokI	GGATG	1 cut(s)	1130
FriOI	GRGCYC	3 cut(s)	19, 42, 801
FspBI	CTAG	3 cut(s)	35, 726, 1047
GlaI	GCGC	2 cut(s)	297, 831
GsuI	CTGGAG	3 cut(s)	311, 1224, 1610
HaeII	RGCGCY	1 cut(s)	833
HaeIII	GGCC	3 cut(s)	223, 305, 963
HapII	CCGG	8 cut(s)	12, 23, 350, 492, 693, 880, 1039, 1069
HhaI	GCGC	2 cut(s)	298, 832
Hin1II	CATG	6 cut(s)	362, 713, 949, 1102, 1261, 1595
Hin6I	GCGC	2 cut(s)	296, 830
HinP1I	GCGC	2 cut(s)	296, 830
HincII	GTYRAC	1 cut(s)	1020
HindII	GTYRAC	1 cut(s)	1020
HindIII	AAGCTT	1 cut(s)	815
HinfI	GANTC	8 cut(s)	186, 278, 355, 683, 706, 850, 988, 1520
HpaII	CCGG	8 cut(s)	12, 23, 350, 492, 693, 880, 1039, 1069
HphI	GGTGA	3 cut(s)	322, 793, 1460
Hpy166II	GTNNAC	3 cut(s)	640, 784, 1020
Hpy188I	TCNGA	9 cut(s)	54, 66, 283, 418, 481, 531, 1525, 1584, 1605
Hpy188III	TCNNGA	9 cut(s)	140, 359, 575, 581, 693, 1241, 1270, 1506, 1627
Hpy8I	GTNNAC	3 cut(s)	640, 784, 1020
Hpy99I	CGWCG	1 cut(s)	70
HpyAV	CCTTC	6 cut(s)	97, 278, 367, 730, 820, 1080
HpyCH4III	ACNGT	2 cut(s)	1284, 1573
HpyCH4IV	ACGT	1 cut(s)	1016
HpyCH4V	TGCA	3 cut(s)	1102, 1280, 1317
HpyF3I	CTNAG	4 cut(s)	18, 123, 582, 1553
HpySE526I	ACGT	1 cut(s)	1016
Hsp92II	CATG	6 cut(s)	362, 713, 949, 1102, 1261, 1595
HspAI	GCGC	2 cut(s)	296, 830
Kpn2I	TCCGGA	1 cut(s)	692
KpnI	GGTACC	1 cut(s)	1491
Ksp22I	TGATCA	1 cut(s)	526
KspI	CCGCGG	1 cut(s)	309
Kzo9I	GATC	6 cut(s)	136, 526, 577, 1149, 1360, 1410
LmnI	GCTCC	3 cut(s)	660, 926, 1457
Lsp1109I	GCAGC	6 cut(s)	84, 468, 550, 983, 1192, 1546
LweI	GCATC	3 cut(s)	58, 758, 1111
MabI	ACCWGGT	1 cut(s)	1263
MaeI	CTAG	3 cut(s)	35, 726, 1047
MaeII	ACGT	1 cut(s)	1016
MaeIII	GTNAC	3 cut(s)	941, 1012, 1324
MalI	GATC	6 cut(s)	138, 528, 579, 1151, 1362, 1412
MboI	GATC	6 cut(s)	136, 526, 577, 1149, 1360, 1410
MboII	GAAGA	9 cut(s)	176, 179, 182, 745, 761, 1154, 1320, 1399, 1541
MfeI	CAATTG	1 cut(s)	1536
MflI	RGATCY	2 cut(s)	1360, 1410
MhlI	GDGCHC	4 cut(s)	19, 42, 204, 801
MlyI	GAGTC	3 cut(s)	715, 997, 1529
MmeI	TCCRAC	1 cut(s)	89
Mph1103I	ATGCAT	1 cut(s)	1104
MroI	TCCGGA	1 cut(s)	692
MseI	TTAA	2 cut(s)	1223, 1304
MspA1I	CMGCKG	3 cut(s)	97, 308, 960
MspI	CCGG	8 cut(s)	12, 23, 350, 492, 693, 880, 1039, 1069
MunI	CAATTG	1 cut(s)	1536
MvaI	CCWGG	4 cut(s)	898, 1265, 1470, 1695
MvnI	CGCG	3 cut(s)	141, 298, 308
NciI	CCSGG	7 cut(s)	13, 24, 350, 492, 880, 881, 1040
NdeI	CATATG	1 cut(s)	702
NdeII	GATC	6 cut(s)	136, 526, 577, 1149, 1360, 1410
NlaIII	CATG	6 cut(s)	362, 713, 949, 1102, 1261, 1595
NlaIV	GGNNCC	6 cut(s)	230, 326, 348, 1044, 1459, 1489
NmeAIII	GCCGAG	1 cut(s)	249
NmuCI	GTSAC	1 cut(s)	941
NruI	TCGCGA	1 cut(s)	141
NsiI	ATGCAT	1 cut(s)	1104
NspI	RCATGY	1 cut(s)	1102
NspV	TTCGAA	1 cut(s)	1654
PaeI	GCATGC	1 cut(s)	1102
PagI	TCATGA	1 cut(s)	358
PasI	CCCWGGG	1 cut(s)	1469
PfeI	GAWTC	5 cut(s)	186, 278, 355, 683, 850
Ple19I	CGATCG	1 cut(s)	139
PleI	GAGTC	3 cut(s)	714, 996, 1528
PpsI	GAGTC	3 cut(s)	714, 996, 1528
PpuMI	RGGWCCY	2 cut(s)	229, 1043
PsiI	TTATAA	1 cut(s)	777
Psp5II	RGGWCCY	2 cut(s)	229, 1043
Psp6I	CCWGG	4 cut(s)	896, 1263, 1468, 1693
PspGI	CCWGG	4 cut(s)	896, 1263, 1468, 1693
PspN4I	GGNNCC	6 cut(s)	230, 326, 348, 1044, 1459, 1489
PspPI	GGNCC	6 cut(s)	229, 303, 346, 1043, 1076, 1184
PspPPI	RGGWCCY	2 cut(s)	229, 1043
PstNI	CAGNNNCTG	1 cut(s)	100
PsuI	RGATCY	2 cut(s)	1360, 1410
PvuI	CGATCG	1 cut(s)	139
PvuII	CAGCTG	1 cut(s)	97
RruI	TCGCGA	1 cut(s)	141
RsaI	GTAC	4 cut(s)	503, 619, 1336, 1489
RsaNI	GTAC	4 cut(s)	502, 618, 1335, 1488
SacII	CCGCGG	1 cut(s)	309
SaqAI	TTAA	2 cut(s)	1223, 1304
Sau3AI	GATC	6 cut(s)	136, 526, 577, 1149, 1360, 1410
Sau96I	GGNCC	6 cut(s)	229, 303, 346, 1043, 1076, 1184
SchI	GAGTC	3 cut(s)	715, 997, 1529
SduI	GDGCHC	4 cut(s)	19, 42, 204, 801
SexAI	ACCWGGT	1 cut(s)	1263
SfaNI	GCATC	3 cut(s)	58, 758, 1111
SfcI	CTRYAG	2 cut(s)	92, 1175
Sfr303I	CCGCGG	1 cut(s)	309
SfuI	TTCGAA	1 cut(s)	1654
SgrBI	CCGCGG	1 cut(s)	309
SinI	GGWCC	5 cut(s)	229, 346, 1043, 1076, 1184
SmaI	CCCGGG	1 cut(s)	881
SmlI	CTYRAG	1 cut(s)	818
SmoI	CTYRAG	1 cut(s)	818
SphI	GCATGC	1 cut(s)	1102
SspI	AATATT	2 cut(s)	115, 756
SspMI	CTAG	3 cut(s)	35, 726, 1047
StyI	CCWWGG	2 cut(s)	1046, 1287
TaaI	ACNGT	2 cut(s)	1284, 1573
TaiI	ACGT	1 cut(s)	1019
TaqI	TCGA	2 cut(s)	402, 1654
TatI	WGTACW	1 cut(s)	617
TauI	GCSGC	7 cut(s)	91, 301, 308, 334, 749, 864, 963
TfiI	GAWTC	5 cut(s)	186, 278, 355, 683, 850
Tru1I	TTAA	2 cut(s)	1223, 1304
Tru9I	TTAA	2 cut(s)	1223, 1304
TscAI	CASTG	1 cut(s)	649
TseFI	GTSAC	1 cut(s)	941
TseI	GCWGC	6 cut(s)	97, 456, 563, 971, 1205, 1559
Tsp45I	GTSAC	1 cut(s)	941
TspDTI	ATGAA	4 cut(s)	347, 375, 678, 1274
TspGWI	ACGGA	3 cut(s)	73, 682, 1470
TspMI	CCCGGG	1 cut(s)	879
TspRI	CASTG	1 cut(s)	649
VpaK11BI	GGWCC	5 cut(s)	229, 346, 1043, 1076, 1184
XagI	CCTNNNNNAGG	1 cut(s)	1050
XapI	RAATTY	2 cut(s)	421, 1610
XceI	RCATGY	1 cut(s)	1102
XcmI	CCANNNNNNNNNTGG	1 cut(s)	268
XmaI	CCCGGG	1 cut(s)	879
XmaJI	CCTAGG	1 cut(s)	1046
XmiI	GTMKAC	1 cut(s)	639
XspI	CTAG	3 cut(s)	35, 726, 1047
Zsp2I	ATGCAT	1 cut(s)	1104

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.