RchiOBHm_Chr7g0221291

Interconversion of serine and glycine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004637
Physical Location & Seq
Forward (+)
42266075 .. 42270835
4761 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19800

Sequence Viewer

Length: 1773 bp
ATGGACTTATCATCATCCTCCCACCCGCAATCCAACCTCTCTTTGGGATTTTCATCCCACGCGCCGTCGTCTCAAATCGCCGATGACTCCATCGCACTCCCCGTAGCCCCATCAATCCCGCTCCAATTGCTCGAGCCACAGCCGGAGAGCCACACAACAACAACAACAACAACTAATGGTGAAATTGGCGAAGACGACGACGACGACGACGACAGAGACGTCGAGGAGTTTCGGATATTGGGGCATTCCATGTGCTTGAAACGCCGCAGGGACAGAGAATCCTCCTCCTCCTGTTCCTCCACCACCAATTCCAAACGCGCGGCACTCTCTGAACCTCCTCCACCACCGGACCTTGAGGCCCGGAGGACTGCTGTCCGGGCCTGGGGTGACCAGCCTCTCCGAGTTGCCGACCCCGATGTGTTTGGGATTATGGACAAGGAGAAGCAGAGGCAGTTCAAAGGGATTGAATTGATTGCCTCGGAGAATTTCGTGTGCCGGGCAGTAATGGAAGCACTTGGAAGCCACTTGACCAACAAGTACTCCGAAGGAATGCCCGGTGCAAGGTACTACGGTGGTAACCAGTACATTGATGAGATCGAAACACTTTGCTGTAAGCGTGCTTTAGCCGCATTTGGTCTCGATGCTGAGAATTGGGGTGTCAATGTGCAGCCTTATTCATGTACATCTGCCAACTTTGCTGTGTACACTGGATTGTTATTGCCCGGTGACAGAATAATGGGGTTGGATACCCCATCTGGAGGGAATACTAGTCATGGATATTATACCAGCAATGGGAGGAAGGTTTCCGGAGCATCAATTTTCTTTGAGACCTTGCCTTATAAGGTCAATTTACAAACTGGGTATATTGATTATGATAAGCTCGAGGAGAGGGCGCTTGATTTTCGCCCCAAGATACTTATTTGCGGTGGGAGTTCGTATCCCCGCGAATGGGATTATGCAAGGTTTAGGCAGATTGCTGATAAATGTGGAGCTGTGCTGCTTTGTGATATGGCGCAAATTAGTGGCCTTATTGCAGCAAAGGAATGTGTAAATCCTTTTGACTACTGTGACATTGTTACTTCTACTACTCATAAAAGCCTTCGGGGTCCCAGGGGAGGTATAATTTTTTATAGGAAGGGTACAAAACCAAGGAAGAGGGGATTTCTTTTAACTCAAGGAAGTGAAAGCGACCACTATGATTTTGAGGAAAAGATCAACTTTGCCGTTTTTCCATCATTGCAAGGGGGACCGCACAATAACCACATTGCTGCTCTTGCCATAGCTTTAAAGCAAGTGGCTACACCTGAATACAAGGCATATATGCAGCAAGTGAAGAAAAACGCACAGGCTTTAGCATCATCTTTGCTGAGAAGAAAGTGCAGGTTAGTAACTGGTGGCACAGACAACCATTTGCTACTTTGGGATCTGAGGCCCCTAGGATTATCAGGCAAGAGCTACGAGAAGGTATGTGAAATGTGTCACATTACTGTCAACAAGATCGCCATTTTTGGTGATAATGGAACTATTACTCCTGGAGGAGTGAGGATTGGTACCCCCGCTATGACTTCAAGAGGTTGTTTGGAGGTGGATTTTGAGACAATTGCTGATTTTCTCCTAAGAGCTGCACAGATTGCAACTTTAGTGCAAAGGGAGCATGGGAAGTTGCAGAAGCCTTTTCTGAAGGGTCTTGAGAGCAATAAGGATATTGTTGAGCTTAGGACCAGAGTTGAAACCTTTGCTTCTCAGTTTGCAATGCCGGGATATGATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523
Pfam Domains
Protein Families
SHMT

Protein Analysis

590

Amino Acids

65.06

Weight (kDa)

6.26

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SHMT PF00464 134 - 537 1.8e-169 Serine hydroxymethyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 840
AasI GACNNNNNNGTC 1 cut(s) 218
AatII GACGTC 1 cut(s) 222
Acc36I ACCTGC 1 cut(s) 1371
Acc65I GGTACC 1 cut(s) 1550
AccB1I GGYRCC 1 cut(s) 1550
AccBSI CCGCTC 1 cut(s) 121
AccII CGCG 4 cut(s) 62, 318, 320, 945
AccIII TCCGGA 1 cut(s) 806
AciI CCGC 9 cut(s) 26, 119, 265, 320, 627, 924, 943, 1250, 1557
AclWI GGATC 1 cut(s) 1431
AcsI RAATTY 1 cut(s) 484
AcuI CTGAAG 1 cut(s) 1700
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 7 cut(s) 539, 566, 584, 682, 704, 1141, 1552
AfiI CCNNNNNNNGG 8 cut(s) 43, 382, 561, 758, 792, 948, 949, 1115
AgsI TTSAA 5 cut(s) 259, 457, 467, 1569, 1730
AhlI ACTAGT 1 cut(s) 767
AjnI CCWGG 3 cut(s) 380, 1109, 1531
AloI GAACNNNNNNTCC 2 cut(s) 1513, 1545
AluBI AGCT 6 cut(s) 880, 992, 1283, 1455, 1622, 1714
AluI AGCT 6 cut(s) 880, 992, 1283, 1455, 1622, 1714
Alw26I GTCTC 5 cut(s) 75, 210, 641, 821, 1589
AlwI GGATC 1 cut(s) 1431
Ama87I CYCGRG 2 cut(s) 131, 881
Aor13HI TCCGGA 1 cut(s) 806
AoxI GGCC 4 cut(s) 357, 378, 1024, 1430
ApeKI GCWGC 6 cut(s) 667, 997, 1034, 1268, 1324, 1622
ApoI RAATTY 1 cut(s) 484
Asp718I GGTACC 1 cut(s) 1550
AspA2I CCTAGG 1 cut(s) 1435
AspLEI GCGC 4 cut(s) 64, 320, 895, 1015
AspS9I GGNCC 7 cut(s) 349, 358, 378, 1106, 1247, 1431, 1719
AsuC2I CCSGG 6 cut(s) 361, 377, 497, 555, 723, 1758
AsuHPI GGTGA 4 cut(s) 191, 398, 737, 1523
AvaI CYCGRG 2 cut(s) 131, 881
AvaII GGWCC 4 cut(s) 349, 1106, 1247, 1719
AvrII CCTAGG 1 cut(s) 1435
BaeI ACNNNNGTAYC 2 cut(s) 556, 589
BanI GGYRCC 1 cut(s) 1550
BbsI GAAGAC 1 cut(s) 198
BbvI GCAGC 6 cut(s) 679, 984, 1046, 1255, 1336, 1609
BccI CCATC 4 cut(s) 98, 118, 760, 1240
BceAI ACGGC 2 cut(s) 49, 1208
BciT130I CCWGG 3 cut(s) 382, 1111, 1533
BciVI GTATCC 2 cut(s) 739, 948
BcnI CCSGG 6 cut(s) 361, 377, 497, 555, 723, 1758
BcoDI GTCTC 5 cut(s) 75, 210, 641, 821, 1589
BcuI ACTAGT 1 cut(s) 767
BfaI CTAG 2 cut(s) 768, 1436
BfoI RGCGCY 1 cut(s) 896
BfuAI ACCTGC 1 cut(s) 1371
BfuI GTATCC 2 cut(s) 739, 948
BisI GCNGC 9 cut(s) 265, 321, 627, 668, 998, 1035, 1269, 1325, 1623
BlnI CCTAGG 1 cut(s) 1435
BlsI GCNGC 9 cut(s) 266, 322, 628, 669, 999, 1036, 1270, 1326, 1624
BmcAI AGTACT 1 cut(s) 539
Bme1390I CCNGG 9 cut(s) 361, 377, 382, 497, 555, 723, 1111, 1533, 1758
Bme18I GGWCC 4 cut(s) 349, 1106, 1247, 1719
BmeT110I CYCGRG 2 cut(s) 131, 881
BmgT120I GGNCC 7 cut(s) 349, 358, 378, 1106, 1247, 1431, 1719
BmiI GGNNCC 5 cut(s) 1107, 1108, 1248, 1433, 1552
BmrFI CCNGG 9 cut(s) 361, 377, 382, 497, 555, 723, 1111, 1533, 1758
BmrI ACTGGG 1 cut(s) 867
BmsI GCATC 3 cut(s) 631, 821, 1364
BmuI ACTGGG 1 cut(s) 867
BoxI GACNNNNGTC 1 cut(s) 371
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 2 cut(s) 777, 1554
Bpu10I CCTNAGC 1 cut(s) 1715
BpuEI CTTGAG 3 cut(s) 374, 1158, 1709
BpuMI CCSGG 6 cut(s) 361, 377, 497, 555, 723, 1758
BsaBI GATNNNNATC 1 cut(s) 52
BsaHI GRCGYC 1 cut(s) 219
BsaI GGTCTC 2 cut(s) 641, 821
BsaJI CCNNGG 6 cut(s) 381, 477, 1109, 1110, 1148, 1435
BsaWI WCCGGW 2 cut(s) 346, 806
BsaXI ACNNNNNCTCC 4 cut(s) 524, 554, 1513, 1543
Bsc4I CCNNNNNNNGG 8 cut(s) 43, 382, 561, 758, 792, 948, 949, 1115
Bse1I ACTGG 4 cut(s) 580, 712, 862, 1396
Bse3DI GCAATG 4 cut(s) 796, 1235, 1263, 1758
Bse8I GATNNNNATC 1 cut(s) 52
BseAI TCCGGA 1 cut(s) 806
BseBI CCWGG 3 cut(s) 382, 1111, 1533
BseDI CCNNGG 6 cut(s) 381, 477, 1109, 1110, 1148, 1435
BseGI GGATG 2 cut(s) 14, 53
BseJI GATNNNNATC 1 cut(s) 52
BseLI CCNNNNNNNGG 8 cut(s) 43, 382, 561, 758, 792, 948, 949, 1115
BseMI GCAATG 4 cut(s) 796, 1235, 1263, 1758
BseMII CTCAG 4 cut(s) 636, 1358, 1418, 1757
BseNI ACTGG 4 cut(s) 580, 712, 862, 1396
BseRI GAGGAG 6 cut(s) 239, 274, 277, 327, 899, 1551
BseXI GCAGC 6 cut(s) 679, 984, 1046, 1255, 1336, 1609
BsgI GTGCAG 3 cut(s) 686, 1399, 1608
Bsh1236I CGCG 4 cut(s) 62, 318, 320, 945
BshFI GGCC 4 cut(s) 359, 380, 1026, 1432
BshNI GGYRCC 1 cut(s) 1550
BsiHKCI CYCGRG 2 cut(s) 131, 881
BsiSI CCGG 9 cut(s) 143, 347, 361, 376, 496, 555, 723, 807, 1757
BslFI GGGAC 3 cut(s) 284, 1092, 1260
BslI CCNNNNNNNGG 8 cut(s) 43, 382, 561, 758, 792, 948, 949, 1115
BsmAI GTCTC 5 cut(s) 75, 210, 641, 821, 1589
BsmBI CGTCTC 2 cut(s) 75, 210
BsmFI GGGAC 3 cut(s) 284, 1092, 1260
BsmI GAATGC 2 cut(s) 244, 555
BsnI GGCC 4 cut(s) 359, 380, 1026, 1432
Bso31I GGTCTC 2 cut(s) 641, 821
BsoBI CYCGRG 2 cut(s) 131, 881
Bsp13I TCCGGA 1 cut(s) 806
Bsp1407I TGTACA 2 cut(s) 680, 702
Bsp143I GATC 4 cut(s) 594, 1212, 1423, 1497
BspACI CCGC 9 cut(s) 26, 119, 265, 320, 627, 924, 943, 1250, 1557
BspANI GGCC 4 cut(s) 359, 380, 1026, 1432
BspCNI CTCAG 4 cut(s) 637, 1359, 1419, 1756
BspEI TCCGGA 1 cut(s) 806
BspFNI CGCG 4 cut(s) 62, 318, 320, 945
BspLI GGNNCC 5 cut(s) 1107, 1108, 1248, 1433, 1552
BspMI ACCTGC 1 cut(s) 1371
BspPI GGATC 1 cut(s) 1431
BspT107I GGYRCC 1 cut(s) 1550
BspTNI GGTCTC 2 cut(s) 641, 821
BsrBI CCGCTC 1 cut(s) 121
BsrDI GCAATG 4 cut(s) 796, 1235, 1263, 1758
BsrGI TGTACA 2 cut(s) 680, 702
BsrI ACTGG 4 cut(s) 580, 712, 862, 1396
BssECI CCNNGG 6 cut(s) 381, 477, 1109, 1110, 1148, 1435
BssMI GATC 4 cut(s) 594, 1212, 1423, 1497
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 2 cut(s) 1148, 1435
Bst2UI CCWGG 3 cut(s) 382, 1111, 1533
Bst4CI ACNGT 3 cut(s) 572, 1067, 1489
Bst6I CTCTTC 1 cut(s) 1148
BstACI GRCGYC 1 cut(s) 219
BstAPI GCANNNNNTGC 1 cut(s) 1631
BstAUI TGTACA 2 cut(s) 680, 702
BstC8I GCNNGC 1 cut(s) 618
BstDEI CTNAG 6 cut(s) 645, 1367, 1427, 1616, 1715, 1743
BstEII GGTNACC 2 cut(s) 386, 575
BstF5I GGATG 2 cut(s) 14, 53
BstFNI CGCG 4 cut(s) 62, 318, 320, 945
BstH2I RGCGCY 1 cut(s) 896
BstHHI GCGC 4 cut(s) 64, 320, 895, 1015
BstKTI GATC 4 cut(s) 597, 1215, 1426, 1500
BstMAI GTCTC 5 cut(s) 75, 210, 641, 821, 1589
BstMBI GATC 4 cut(s) 594, 1212, 1423, 1497
BstMWI GCNNNNNNNGC 8 cut(s) 127, 261, 377, 626, 695, 1274, 1631, 1651
BstNI CCWGG 3 cut(s) 382, 1111, 1533
BstPAI GACNNNNGTC 1 cut(s) 371
BstPI GGTNACC 2 cut(s) 386, 575
BstSCI CCNGG 9 cut(s) 359, 375, 380, 495, 553, 721, 1109, 1531, 1756
BstUI CGCG 4 cut(s) 62, 318, 320, 945
BstV1I GCAGC 6 cut(s) 679, 984, 1046, 1255, 1336, 1609
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 1423
BstYI RGATCY 1 cut(s) 1423
BsuI GTATCC 2 cut(s) 739, 948
BsuRI GGCC 4 cut(s) 359, 380, 1026, 1432
BtgZI GCGATG 1 cut(s) 76
BtsCI GGATG 2 cut(s) 14, 53
BtsIMutI CAGTG 1 cut(s) 705
BveI ACCTGC 1 cut(s) 1371
Cac8I GCNNGC 1 cut(s) 618
CfoI GCGC 4 cut(s) 64, 320, 895, 1015
Cfr13I GGNCC 7 cut(s) 349, 358, 378, 1106, 1247, 1431, 1719
Csp6I GTAC 7 cut(s) 538, 565, 583, 681, 703, 1140, 1551
CviAII CATG 4 cut(s) 250, 678, 773, 1655
CviQI GTAC 7 cut(s) 538, 565, 583, 681, 703, 1140, 1551
DdeI CTNAG 6 cut(s) 645, 1367, 1427, 1616, 1715, 1743
DpnI GATC 4 cut(s) 596, 1214, 1425, 1499
DpnII GATC 4 cut(s) 594, 1212, 1423, 1497
DraI TTTAAA 1 cut(s) 1287
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
Eam1104I CTCTTC 1 cut(s) 1148
EarI CTCTTC 1 cut(s) 1148
Eco130I CCWWGG 2 cut(s) 1148, 1435
Eco31I GGTCTC 2 cut(s) 641, 821
Eco47I GGWCC 4 cut(s) 349, 1106, 1247, 1719
Eco57I CTGAAG 1 cut(s) 1700
Eco88I CYCGRG 2 cut(s) 131, 881
Eco91I GGTNACC 2 cut(s) 386, 575
EcoO109I RGGNCCY 2 cut(s) 1106, 1431
EcoO65I GGTNACC 2 cut(s) 386, 575
EcoRII CCWGG 3 cut(s) 380, 1109, 1531
EcoT14I CCWWGG 2 cut(s) 1148, 1435
ErhI CCWWGG 2 cut(s) 1148, 1435
Esp3I CGTCTC 2 cut(s) 75, 210
FaeI CATG 4 cut(s) 253, 681, 776, 1658
FalI AAGNNNNNCTT 2 cut(s) 1202, 1234
FaqI GGGAC 3 cut(s) 284, 1092, 1260
FatI CATG 4 cut(s) 249, 677, 772, 1654
FauI CCCGC 4 cut(s) 33, 126, 950, 1564
Fnu4HI GCNGC 9 cut(s) 265, 321, 627, 668, 998, 1035, 1269, 1325, 1623
FokI GGATG 1 cut(s) 40
Fsp4HI GCNGC 9 cut(s) 265, 321, 627, 668, 998, 1035, 1269, 1325, 1623
FspBI CTAG 2 cut(s) 768, 1436
GlaI GCGC 4 cut(s) 63, 319, 894, 1014
GluI GCNGC 9 cut(s) 265, 321, 627, 668, 998, 1035, 1269, 1325, 1623
GsuI CTGGAG 2 cut(s) 777, 1554
HaeII RGCGCY 1 cut(s) 896
HaeIII GGCC 4 cut(s) 359, 380, 1026, 1432
HapII CCGG 9 cut(s) 143, 347, 361, 376, 496, 555, 723, 807, 1757
HhaI GCGC 4 cut(s) 64, 320, 895, 1015
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 4 cut(s) 253, 681, 776, 1658
Hin6I GCGC 4 cut(s) 62, 318, 893, 1013
HinP1I GCGC 4 cut(s) 62, 318, 893, 1013
HincII GTYRAC 1 cut(s) 1492
HindII GTYRAC 1 cut(s) 1492
HinfI GANTC 2 cut(s) 86, 278
HpaII CCGG 9 cut(s) 143, 347, 361, 376, 496, 555, 723, 807, 1757
HphI GGTGA 4 cut(s) 191, 398, 737, 1523
Hpy166II GTNNAC 3 cut(s) 703, 705, 1492
Hpy188I TCNGA 7 cut(s) 234, 331, 401, 481, 544, 1428, 1680
Hpy188III TCNNGA 5 cut(s) 638, 756, 807, 1569, 1688
Hpy8I GTNNAC 3 cut(s) 703, 705, 1492
Hpy99I CGWCG 7 cut(s) 70, 200, 203, 206, 209, 212, 224
HpyAV CCTTC 6 cut(s) 539, 793, 1109, 1129, 1456, 1675
HpyCH4III ACNGT 3 cut(s) 572, 1067, 1489
HpyCH4IV ACGT 1 cut(s) 219
HpyF10VI GCNNNNNNNGC 8 cut(s) 127, 261, 377, 626, 695, 1274, 1631, 1651
HpyF3I CTNAG 6 cut(s) 645, 1367, 1427, 1616, 1715, 1743
HpySE526I ACGT 1 cut(s) 219
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 4 cut(s) 253, 681, 776, 1658
HspAI GCGC 4 cut(s) 62, 318, 893, 1013
KflI GGGWCCC 1 cut(s) 1106
Kpn2I TCCGGA 1 cut(s) 806
KpnI GGTACC 1 cut(s) 1554
Kzo9I GATC 4 cut(s) 594, 1212, 1423, 1497
LmnI GCTCC 4 cut(s) 126, 809, 989, 1651
Lsp1109I GCAGC 6 cut(s) 679, 984, 1046, 1255, 1336, 1609
LweI GCATC 3 cut(s) 631, 821, 1364
MaeI CTAG 2 cut(s) 768, 1436
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 7 cut(s) 386, 575, 725, 1067, 1075, 1387, 1478
MalI GATC 4 cut(s) 596, 1214, 1425, 1499
MbiI CCGCTC 1 cut(s) 121
MboI GATC 4 cut(s) 594, 1212, 1423, 1497
MboII GAAGA 4 cut(s) 203, 1165, 1345, 1383
MfeI CAATTG 2 cut(s) 125, 1599
MflI RGATCY 1 cut(s) 1423
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 2 cut(s) 57, 723
MroI TCCGGA 1 cut(s) 806
MseI TTAA 2 cut(s) 1169, 1286
MspI CCGG 9 cut(s) 143, 347, 361, 376, 496, 555, 723, 807, 1757
MspR9I CCNGG 9 cut(s) 361, 377, 382, 497, 555, 723, 1111, 1533, 1758
MunI CAATTG 2 cut(s) 125, 1599
Mva1269I GAATGC 2 cut(s) 244, 555
MvaI CCWGG 3 cut(s) 382, 1111, 1533
MvnI CGCG 4 cut(s) 62, 318, 320, 945
MwoI GCNNNNNNNGC 8 cut(s) 127, 261, 377, 626, 695, 1274, 1631, 1651
NciI CCSGG 6 cut(s) 361, 377, 497, 555, 723, 1758
NdeII GATC 4 cut(s) 594, 1212, 1423, 1497
NlaIII CATG 4 cut(s) 253, 681, 776, 1658
NlaIV GGNNCC 5 cut(s) 1107, 1108, 1248, 1433, 1552
NmuCI GTSAC 4 cut(s) 386, 725, 1067, 1478
PaeR7I CTCGAG 2 cut(s) 131, 881
PasI CCCWGGG 1 cut(s) 1110
PcsI WCGNNNNNNNCGW 5 cut(s) 99, 201, 204, 207, 216
PctI GAATGC 2 cut(s) 244, 555
PfeI GAWTC 1 cut(s) 278
PfoI TCCNGGA 1 cut(s) 1531
PkrI GCNGC 9 cut(s) 266, 322, 628, 669, 999, 1036, 1270, 1326, 1624
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
PpuMI RGGWCCY 1 cut(s) 1106
PshAI GACNNNNGTC 1 cut(s) 371
PsiI TTATAA 1 cut(s) 840
Psp5II RGGWCCY 1 cut(s) 1106
Psp6I CCWGG 3 cut(s) 380, 1109, 1531
PspEI GGTNACC 2 cut(s) 386, 575
PspGI CCWGG 3 cut(s) 380, 1109, 1531
PspN4I GGNNCC 5 cut(s) 1107, 1108, 1248, 1433, 1552
PspPI GGNCC 7 cut(s) 349, 358, 378, 1106, 1247, 1431, 1719
PspPPI RGGWCCY 1 cut(s) 1106
PspXI VCTCGAGB 2 cut(s) 131, 881
PsuI RGATCY 1 cut(s) 1423
RsaI GTAC 7 cut(s) 539, 566, 584, 682, 704, 1141, 1552
RsaNI GTAC 7 cut(s) 538, 565, 583, 681, 703, 1140, 1551
SaqAI TTAA 2 cut(s) 1169, 1286
SatI GCNGC 9 cut(s) 265, 321, 627, 668, 998, 1035, 1269, 1325, 1623
Sau3AI GATC 4 cut(s) 594, 1212, 1423, 1497
Sau96I GGNCC 7 cut(s) 349, 358, 378, 1106, 1247, 1431, 1719
ScaI AGTACT 1 cut(s) 539
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 9 cut(s) 361, 377, 382, 497, 555, 723, 1111, 1533, 1758
SfaNI GCATC 3 cut(s) 631, 821, 1364
Sfr274I CTCGAG 2 cut(s) 131, 881
SinI GGWCC 4 cut(s) 349, 1106, 1247, 1719
SlaI CTCGAG 2 cut(s) 131, 881
SmlI CTYRAG 5 cut(s) 131, 353, 881, 1173, 1688
SmoI CTYRAG 5 cut(s) 131, 353, 881, 1173, 1688
SpeI ACTAGT 1 cut(s) 767
SsiI CCGC 9 cut(s) 26, 119, 265, 320, 627, 924, 943, 1250, 1557
SspMI CTAG 2 cut(s) 768, 1436
StyD4I CCNGG 9 cut(s) 359, 375, 380, 495, 553, 721, 1109, 1531, 1756
StyI CCWWGG 2 cut(s) 1148, 1435
TaaI ACNGT 3 cut(s) 572, 1067, 1489
TaiI ACGT 1 cut(s) 222
TaqI TCGA 5 cut(s) 132, 222, 597, 639, 882
TatI WGTACW 4 cut(s) 537, 582, 680, 702
TauI GCSGC 3 cut(s) 267, 323, 629
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 2 cut(s) 1169, 1286
Tru9I TTAA 2 cut(s) 1169, 1286
TscAI CASTG 1 cut(s) 712
TseFI GTSAC 4 cut(s) 386, 725, 1067, 1478
TseI GCWGC 6 cut(s) 667, 997, 1034, 1268, 1324, 1622
Tsp45I GTSAC 4 cut(s) 386, 725, 1067, 1478
TspDTI ATGAA 2 cut(s) 42, 666
TspRI CASTG 1 cut(s) 712
VpaK11BI GGWCC 4 cut(s) 349, 1106, 1247, 1719
XapI RAATTY 1 cut(s) 484
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
XhoI CTCGAG 2 cut(s) 131, 881
XmaJI CCTAGG 1 cut(s) 1435
XspI CTAG 2 cut(s) 768, 1436
ZraI GACGTC 1 cut(s) 220
ZrmI AGTACT 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.