Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0004637

Physical Location & Seq

Genomic Coordinates Forward (+)

22466937 .. 22479369

Gene Structure 12433 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1145600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1677 bp

ATGGGGTTAGCGGAGGCTCAGCCGGGCCTATCTCTAGGGTTTAGCTCGCATCAGAGTACGAGCTCGTCGAATTCAATCCAATCCATTCCGCTGCAATTGCTAGACCAGAAAGAAGATAAAATTAGGGTTGAGAATGGGGATGATCGTGACGAGGACGAGAAATTCAACCTCTTAGGGCACTCAATCTGTCTGAAAAGGCAGCGGGATGGGTTCACTATGAACCCGAACCCGTCAAAGTGCTTGGCGGTGGAATCGGCGGGGCTGGAGGCGAGGCGGGCCGTGGTTCGGTCTTGGGGGAATCAGCCCCTTTCGGTTGCGGACCCGGAGGTCCATGAAATTATGGAGAAGGAGAAGCATCGGCAGTTCAAAGGTATTGAATTGGTTGCTTCTGAGAATTTCGTGTGCAGAGCTGTGATGGAAGCTCTGGGCAGTCACTTGACGAATAAGTACTCGGAGGGTATGCCCGGGGCGAGATACTACACGGGCAATCAGCATATTGATCAGATTGAATTGCTTTGTCATGAACGCGCATTGGCAGCTTTTGGTCTTGATCCAGAGAAATGGGGCGTCAATGTCCAGCCTTATTCTTGTACCTCTGCGAATTTCGCAGTCTACACCGGTCTTTTGCTCCCAAATGATAGGATTATGGGATTGGATTCGCCTTCGGGAGGGCATATGAGTCATGGGTATTATATTCCTGGTGGGAAGAATGTATCGGCTGCCTCAATATTCTTTGATAGTTTCCCTTATAAGGTGAACCCACAAACAGGGTACATTGATTATGATAAGCTTGAGGAGAGGGCGGTTGATTATCGACCCAAGATACTTATTTGCGGTGGGAGTTCATACCCCCGGGAGTGGGATTATGCCAGGTTTAGGCATGTTGCGGATAAATGCGGAGCTGTGTTGATGTGTGACATGGCTCATATCAGTGGTCTTGTGGCAGCTAAGGAATGTGCAAGCCCATTTGATTATTGTGATATTGTTACGTCAACAACGCACAAAAGTCTCCGGGGTCCTAGGGGAGGTATTATTTTTTACAGGAAAGGCACAAAATTAAGGAAGCAAGGTGTACATCATACTAATGATGGTGATAACAATTATGATTTCGAGGAAAAGATAAACTTCGCTGTTTTCCCCTCCCTACAAGGAGGTCCTCACAACAATTATATTGCTGCTCTTGCCATTGCCTTAAAACAAGTAGCCACTCCAGAGTATAAAGCATACATGAACCAGGTGAAGAGAAATGCACAAGCCTTGGCTAATGCTTTGTTAAGAAGAAAATGCAGATTGGTTACTGGGGGTACTGATAATCACTTGCTGCTTTGGGATCTGACTAGTCTTGGTATAACAGGTAAGAACTTCGAAAAGGTCTGCGAGTTGTGTGACATCACTCTCAATAAAACTGCTATATTTGGTGATAATGGTGCTCCTTCTCGTCGGGGAGTGAGAATTGGTACTCCTGCTATGACTACAAGAGGTTGTGTGGAGGCCGATTTTGAAACAATTGCAGACTTTCTTCTAAGGGCTGCGAAGATCACTGCTTCTGTCCAGCTGGAACGTGGAACATATCATAAAGATTTTATCCAAGGTCTCCAGAACAACAAAGATATTGTTGAGCTCAGAAATCGGGTCGAAACATTTGCGTACCAGTTTGAAATGCCAGGATATGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004372 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005759 GO:0006730 GO:0006732 GO:0006790 GO:0008150 GO:0008152 GO:0009987 GO:0010035 GO:0010038 GO:0016740 GO:0016741 GO:0016742 GO:0017144 GO:0019219 GO:0019222 GO:0031323 GO:0031974 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044030 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046500 GO:0046686 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051052 GO:0051171 GO:0051186 GO:0055063 GO:0055081 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0072505 GO:0080090 GO:0098771

KEGG Pathways

Metabolic & Signaling

ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523

Pfam Domains

Protein Families

SHMT

Protein Analysis

559

Amino Acids

62.03

Weight (kDa)

6.79

Isoelectric Point (pI)

39.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SHMT	PF00464	104 - 506	6e-171	Serine hydroxymethyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004637)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22020 AT1G22020 AT1G36370
fragaria_vesca	FvH4_5g28970 FvH4_5g38450 FvH4_5g38450
malus_domestica	MD04G1132900.v1.1 MD12G1145600.v1.1
prunus_persica	Prupe.1G507500_v2.0.a1 Prupe.1G507600_v2.0.a1
pyrus_communis	pycom04g12080 pycom12g13990 pycom15g32210
rosa_chinensis	RchiOBHm_Chr7g0221291 RchiOBHm_Chr7g0240491
rosa_laevigata	RLG00000000675 RLG00000002164
rosa_multiflora	Rmu_sc0000324.1_g000020 Rmu_sc0005327.1_g000009
rosa_roxburghii	Rroxscaffold_3G00219940 Rroxscaffold_3G00238440
rosa_rugosa	Rorug07G0202000 Rorug07G0336600
rosa_samantha	Rh7AG341100 Rh7AG491000 Rh7BG332100 Rh7BG463500 Rh7CG359000 Rh7CG507100
rosa_wichuraiana	Rw7G029010 Rw7G041140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	750
AasI	GACNNNNNNGTC	1 cut(s)	326
AccI	GTMKAC	1 cut(s)	612
AccII	CGCG	1 cut(s)	528
AclWI	GGATC	2 cut(s)	545, 1338
AcsI	RAATTY	4 cut(s)	70, 161, 394, 601
AcyI	GRCGYC	1 cut(s)	567
AfaI	GTAC	8 cut(s)	58, 449, 592, 773, 1074, 1306, 1459, 1649
AfiI	CCNNNNNNNGG	8 cut(s)	285, 668, 751, 767, 858, 859, 876, 1025
AgeI	ACCGGT	1 cut(s)	617
AgsI	TTSAA	7 cut(s)	75, 166, 367, 377, 509, 1502, 1658
AhlI	ACTAGT	1 cut(s)	1337
AjnI	CCWGG	4 cut(s)	697, 869, 1233, 1663
Alw21I	GWGCWC	3 cut(s)	65, 1432, 1623
Alw26I	GTCTC	2 cut(s)	1013, 1598
AlwI	GGATC	2 cut(s)	545, 1338
Ama87I	CYCGRG	2 cut(s)	464, 852
AoxI	GGCC	3 cut(s)	25, 276, 1491
ApeKI	GCWGC	8 cut(s)	91, 199, 536, 719, 944, 1175, 1321, 1529
ApoI	RAATTY	4 cut(s)	70, 161, 394, 601
AsiGI	ACCGGT	1 cut(s)	617
AspA2I	CCTAGG	1 cut(s)	1019
AspLEI	GCGC	1 cut(s)	530
AspS9I	GGNCC	6 cut(s)	25, 276, 319, 328, 1016, 1154
AsuC2I	CCSGG	7 cut(s)	24, 323, 465, 466, 853, 854, 1013
AsuHPI	GGTGA	4 cut(s)	766, 1103, 1249, 1430
AsuII	TTCGAA	1 cut(s)	1365
AvaI	CYCGRG	2 cut(s)	464, 852
AvaII	GGWCC	4 cut(s)	319, 328, 1016, 1154
AvrII	CCTAGG	1 cut(s)	1019
BaeGI	GKGCMC	1 cut(s)	180
BanII	GRGCYC	2 cut(s)	65, 1623
Bbv12I	GWGCWC	3 cut(s)	65, 1432, 1623
BbvI	GCAGC	8 cut(s)	78, 211, 548, 706, 956, 1162, 1308, 1516
BccI	CCATC	3 cut(s)	200, 409, 1082
BceAI	ACGGC	1 cut(s)	263
BcgI	CGANNNNNNTGC	2 cut(s)	1625, 1659
BciT130I	CCWGG	4 cut(s)	699, 871, 1235, 1665
BclI	TGATCA	1 cut(s)	499
BcnI	CCSGG	7 cut(s)	24, 323, 465, 466, 853, 854, 1013
BcoDI	GTCTC	2 cut(s)	1013, 1598
BcuI	ACTAGT	1 cut(s)	1337
BfaI	CTAG	4 cut(s)	35, 101, 1020, 1338
BisI	GCNGC	8 cut(s)	92, 200, 537, 720, 945, 1176, 1322, 1530
BlnI	CCTAGG	1 cut(s)	1019
BlpI	GCTNAGC	1 cut(s)	18
BlsI	GCNGC	8 cut(s)	93, 201, 538, 721, 946, 1177, 1323, 1531
BmcAI	AGTACT	1 cut(s)	449
Bme18I	GGWCC	4 cut(s)	319, 328, 1016, 1154
BmeT110I	CYCGRG	2 cut(s)	464, 852
BmgT120I	GGNCC	6 cut(s)	25, 276, 319, 328, 1016, 1154
BmiI	GGNNCC	2 cut(s)	321, 1017
BmrI	ACTGGG	1 cut(s)	1308
BmsI	GCATC	2 cut(s)	58, 364
BmuI	ACTGGG	1 cut(s)	1308
BpmI	CTGGAG	3 cut(s)	284, 1194, 1580
Bpu10I	CCTNAGC	1 cut(s)	948
Bpu1102I	GCTNAGC	1 cut(s)	18
Bpu14I	TTCGAA	1 cut(s)	1365
BpuEI	CTTGAG	1 cut(s)	812
BpuMI	CCSGG	7 cut(s)	24, 323, 465, 466, 853, 854, 1013
BsaHI	GRCGYC	1 cut(s)	567
BsaI	GGTCTC	1 cut(s)	1598
BsaJI	CCNNGG	9 cut(s)	279, 464, 465, 851, 852, 1012, 1019, 1257, 1588
BsaWI	WCCGGW	1 cut(s)	617
Bsc4I	CCNNNNNNNGG	8 cut(s)	285, 668, 751, 767, 858, 859, 876, 1025
Bse118I	RCCGGY	1 cut(s)	617
Bse1I	ACTGG	2 cut(s)	1303, 1651
Bse3DI	GCAATG	1 cut(s)	1185
BseBI	CCWGG	4 cut(s)	699, 871, 1235, 1665
BseDI	CCNNGG	9 cut(s)	279, 464, 465, 851, 852, 1012, 1019, 1257, 1588
BseGI	GGATG	2 cut(s)	145, 211
BseLI	CCNNNNNNNGG	8 cut(s)	285, 668, 751, 767, 858, 859, 876, 1025
BseMI	GCAATG	1 cut(s)	1185
BseMII	CTCAG	3 cut(s)	32, 381, 1636
BseNI	ACTGG	2 cut(s)	1303, 1651
BseRI	GAGGAG	1 cut(s)	809
BseSI	GKGCMC	1 cut(s)	180
BseXI	GCAGC	8 cut(s)	78, 211, 548, 706, 956, 1162, 1308, 1516
BsgI	GTGCAG	1 cut(s)	424
Bsh1236I	CGCG	1 cut(s)	528
BshFI	GGCC	3 cut(s)	27, 278, 1493
BshTI	ACCGGT	1 cut(s)	617
BsiHKAI	GWGCWC	3 cut(s)	65, 1432, 1623
BsiHKCI	CYCGRG	2 cut(s)	464, 852
BsiSI	CCGG	6 cut(s)	23, 323, 465, 618, 853, 1012
BslI	CCNNNNNNNGG	8 cut(s)	285, 668, 751, 767, 858, 859, 876, 1025
BsmAI	GTCTC	2 cut(s)	1013, 1598
BsnI	GGCC	3 cut(s)	27, 278, 1493
Bso31I	GGTCTC	1 cut(s)	1598
BsoBI	CYCGRG	2 cut(s)	464, 852
Bsp119I	TTCGAA	1 cut(s)	1365
Bsp1286I	GDGCHC	4 cut(s)	65, 180, 1432, 1623
Bsp1407I	TGTACA	1 cut(s)	1072
Bsp143I	GATC	5 cut(s)	142, 499, 550, 1330, 1536
Bsp1720I	GCTNAGC	1 cut(s)	18
BspANI	GGCC	3 cut(s)	27, 278, 1493
BspCNI	CTCAG	3 cut(s)	31, 382, 1635
BspFNI	CGCG	1 cut(s)	528
BspHI	TCATGA	1 cut(s)	520
BspLI	GGNNCC	2 cut(s)	321, 1017
BspPI	GGATC	2 cut(s)	545, 1338
BspT104I	TTCGAA	1 cut(s)	1365
BspTNI	GGTCTC	1 cut(s)	1598
BsrDI	GCAATG	1 cut(s)	1185
BsrFI	RCCGGY	1 cut(s)	617
BsrGI	TGTACA	1 cut(s)	1072
BsrI	ACTGG	2 cut(s)	1303, 1651
BssAI	RCCGGY	1 cut(s)	617
BssECI	CCNNGG	9 cut(s)	279, 464, 465, 851, 852, 1012, 1019, 1257, 1588
BssMI	GATC	5 cut(s)	142, 499, 550, 1330, 1536
BssNI	GRCGYC	1 cut(s)	567
BssT1I	CCWWGG	3 cut(s)	1019, 1257, 1588
Bst2UI	CCWGG	4 cut(s)	699, 871, 1235, 1665
Bst6I	CTCTTC	1 cut(s)	1235
BstACI	GRCGYC	1 cut(s)	567
BstAUI	TGTACA	1 cut(s)	1072
BstBI	TTCGAA	1 cut(s)	1365
BstC8I	GCNNGC	3 cut(s)	47, 276, 961
BstDEI	CTNAG	6 cut(s)	18, 172, 390, 948, 1523, 1622
BstDSI	CCRYGG	1 cut(s)	279
BstENI	CCTNNNNNAGG	2 cut(s)	666, 1023
BstF5I	GGATG	2 cut(s)	145, 211
BstFNI	CGCG	1 cut(s)	528
BstHHI	GCGC	1 cut(s)	530
BstKTI	GATC	5 cut(s)	145, 502, 553, 1333, 1539
BstMAI	GTCTC	2 cut(s)	1013, 1598
BstMBI	GATC	5 cut(s)	142, 499, 550, 1330, 1536
BstMWI	GCNNNNNNNGC	5 cut(s)	97, 275, 536, 605, 1181
BstNI	CCWGG	4 cut(s)	699, 871, 1235, 1665
BstNSI	RCATGY	1 cut(s)	884
BstSLI	GKGCMC	1 cut(s)	180
BstUI	CGCG	1 cut(s)	528
BstV1I	GCAGC	8 cut(s)	78, 211, 548, 706, 956, 1162, 1308, 1516
BstX2I	RGATCY	1 cut(s)	1330
BstXI	CCANNNNNNTGG	1 cut(s)	561
BstYI	RGATCY	1 cut(s)	1330
BsuRI	GGCC	3 cut(s)	27, 278, 1493
BtgI	CCRYGG	1 cut(s)	279
BtsCI	GGATG	2 cut(s)	145, 211
BtsI	GCAGTG	1 cut(s)	1539
BtsIMutI	CAGTG	2 cut(s)	937, 1539
Cac8I	GCNNGC	3 cut(s)	47, 276, 961
CciI	TCATGA	1 cut(s)	520
CfoI	GCGC	1 cut(s)	530
Cfr10I	RCCGGY	1 cut(s)	617
Cfr13I	GGNCC	6 cut(s)	25, 276, 319, 328, 1016, 1154
Cfr9I	CCCGGG	2 cut(s)	464, 852
CseI	GACGC	1 cut(s)	556
CsiI	ACCWGGT	1 cut(s)	1233
Csp6I	GTAC	8 cut(s)	57, 448, 591, 772, 1073, 1305, 1458, 1648
CspAI	ACCGGT	1 cut(s)	617
CviAII	CATG	6 cut(s)	332, 521, 683, 881, 919, 1228
CviQI	GTAC	8 cut(s)	57, 448, 591, 772, 1073, 1305, 1458, 1648
DdeI	CTNAG	6 cut(s)	18, 172, 390, 948, 1523, 1622
DpnI	GATC	5 cut(s)	144, 501, 552, 1332, 1538
DpnII	GATC	5 cut(s)	142, 499, 550, 1330, 1536
DrdI	GACNNNNNNGTC	1 cut(s)	326
DseDI	GACNNNNNNGTC	1 cut(s)	326
Eam1104I	CTCTTC	1 cut(s)	1235
EarI	CTCTTC	1 cut(s)	1235
Ecl136II	GAGCTC	2 cut(s)	63, 1621
Eco130I	CCWWGG	3 cut(s)	1019, 1257, 1588
Eco24I	GRGCYC	2 cut(s)	65, 1623
Eco31I	GGTCTC	1 cut(s)	1598
Eco47I	GGWCC	4 cut(s)	319, 328, 1016, 1154
Eco53kI	GAGCTC	2 cut(s)	63, 1621
Eco88I	CYCGRG	2 cut(s)	464, 852
EcoICRI	GAGCTC	2 cut(s)	63, 1621
EcoNI	CCTNNNNNAGG	2 cut(s)	666, 1023
EcoO109I	RGGNCCY	2 cut(s)	1016, 1154
EcoRI	GAATTC	1 cut(s)	70
EcoRII	CCWGG	4 cut(s)	697, 869, 1233, 1663
EcoT14I	CCWWGG	3 cut(s)	1019, 1257, 1588
EcoT38I	GRGCYC	2 cut(s)	65, 1623
ErhI	CCWWGG	3 cut(s)	1019, 1257, 1588
FaeI	CATG	6 cut(s)	335, 524, 686, 884, 922, 1231
FalI	AAGNNNNNCTT	2 cut(s)	1109, 1141
FatI	CATG	6 cut(s)	331, 520, 682, 880, 918, 1227
FauI	CCCGC	3 cut(s)	195, 250, 267
FauNDI	CATATG	1 cut(s)	675
FbaI	TGATCA	1 cut(s)	499
FblI	GTMKAC	1 cut(s)	612
Fnu4HI	GCNGC	8 cut(s)	92, 200, 537, 720, 945, 1176, 1322, 1530
FokI	GGATG	2 cut(s)	152, 218
FriOI	GRGCYC	2 cut(s)	65, 1623
Fsp4HI	GCNGC	8 cut(s)	92, 200, 537, 720, 945, 1176, 1322, 1530
FspBI	CTAG	4 cut(s)	35, 101, 1020, 1338
GlaI	GCGC	1 cut(s)	529
GluI	GCNGC	8 cut(s)	92, 200, 537, 720, 945, 1176, 1322, 1530
GsuI	CTGGAG	3 cut(s)	284, 1194, 1580
HaeIII	GGCC	3 cut(s)	27, 278, 1493
HapII	CCGG	6 cut(s)	23, 323, 465, 618, 853, 1012
HgaI	GACGC	1 cut(s)	556
HhaI	GCGC	1 cut(s)	530
Hin1I	GRCGYC	1 cut(s)	567
Hin1II	CATG	6 cut(s)	335, 524, 686, 884, 922, 1231
Hin6I	GCGC	1 cut(s)	528
HinP1I	GCGC	1 cut(s)	528
HincII	GTYRAC	1 cut(s)	993
HindII	GTYRAC	1 cut(s)	993
HindIII	AAGCTT	1 cut(s)	788
HinfI	GANTC	4 cut(s)	251, 298, 656, 679
HpaII	CCGG	6 cut(s)	23, 323, 465, 618, 853, 1012
HphI	GGTGA	4 cut(s)	766, 1103, 1249, 1430
Hpy166II	GTNNAC	5 cut(s)	213, 613, 757, 993, 1073
Hpy188I	TCNGA	7 cut(s)	54, 192, 391, 454, 504, 1335, 1625
Hpy188III	TCNNGA	7 cut(s)	146, 521, 548, 554, 666, 1211, 1597
Hpy8I	GTNNAC	5 cut(s)	213, 613, 757, 993, 1073
Hpy99I	CGWCG	2 cut(s)	70, 1443
HpyAV	CCTTC	3 cut(s)	340, 672, 1443
HpyCH4IV	ACGT	2 cut(s)	989, 1561
HpyCH4V	TGCA	6 cut(s)	94, 405, 959, 1250, 1287, 1511
HpyF10VI	GCNNNNNNNGC	5 cut(s)	97, 275, 536, 605, 1181
HpyF3I	CTNAG	6 cut(s)	18, 172, 390, 948, 1523, 1622
HpySE526I	ACGT	2 cut(s)	989, 1561
Hsp92I	GRCGYC	1 cut(s)	567
Hsp92II	CATG	6 cut(s)	335, 524, 686, 884, 922, 1231
HspAI	GCGC	1 cut(s)	528
Ksp22I	TGATCA	1 cut(s)	499
Kzo9I	GATC	5 cut(s)	142, 499, 550, 1330, 1536
LmnI	GCTCC	3 cut(s)	633, 899, 1435
Lsp1109I	GCAGC	8 cut(s)	78, 211, 548, 706, 956, 1162, 1308, 1516
LweI	GCATC	2 cut(s)	58, 364
MabI	ACCWGGT	1 cut(s)	1233
MaeI	CTAG	4 cut(s)	35, 101, 1020, 1338
MaeII	ACGT	2 cut(s)	989, 1561
MaeIII	GTNAC	6 cut(s)	146, 431, 914, 985, 1294, 1385
MalI	GATC	5 cut(s)	144, 501, 552, 1332, 1538
MboI	GATC	5 cut(s)	142, 499, 550, 1330, 1536
MboII	GAAGA	6 cut(s)	125, 718, 1252, 1290, 1511, 1546
MfeI	CAATTG	2 cut(s)	95, 1506
MflI	RGATCY	1 cut(s)	1330
MhlI	GDGCHC	4 cut(s)	65, 180, 1432, 1623
MlyI	GAGTC	1 cut(s)	688
MseI	TTAA	3 cut(s)	1058, 1193, 1274
MslI	CAYNNNNRTG	2 cut(s)	930, 1083
MspA1I	CMGCKG	3 cut(s)	91, 202, 1555
MspI	CCGG	6 cut(s)	23, 323, 465, 618, 853, 1012
MunI	CAATTG	2 cut(s)	95, 1506
MvaI	CCWGG	4 cut(s)	699, 871, 1235, 1665
MvnI	CGCG	1 cut(s)	528
MwoI	GCNNNNNNNGC	5 cut(s)	97, 275, 536, 605, 1181
NciI	CCSGG	7 cut(s)	24, 323, 465, 466, 853, 854, 1013
NdeI	CATATG	1 cut(s)	675
NdeII	GATC	5 cut(s)	142, 499, 550, 1330, 1536
NlaIII	CATG	6 cut(s)	335, 524, 686, 884, 922, 1231
NlaIV	GGNNCC	2 cut(s)	321, 1017
NmuCI	GTSAC	4 cut(s)	146, 431, 914, 1385
NspI	RCATGY	1 cut(s)	884
NspV	TTCGAA	1 cut(s)	1365
PagI	TCATGA	1 cut(s)	520
PcsI	WCGNNNNNNNCGW	1 cut(s)	65
PfeI	GAWTC	3 cut(s)	251, 298, 656
PinAI	ACCGGT	1 cut(s)	617
PkrI	GCNGC	8 cut(s)	93, 201, 538, 721, 946, 1177, 1323, 1531
PleI	GAGTC	1 cut(s)	687
PpsI	GAGTC	1 cut(s)	687
PpuMI	RGGWCCY	2 cut(s)	1016, 1154
PsiI	TTATAA	1 cut(s)	750
Psp124BI	GAGCTC	2 cut(s)	65, 1623
Psp5II	RGGWCCY	2 cut(s)	1016, 1154
Psp6I	CCWGG	4 cut(s)	697, 869, 1233, 1663
PspGI	CCWGG	4 cut(s)	697, 869, 1233, 1663
PspN4I	GGNNCC	2 cut(s)	321, 1017
PspPI	GGNCC	6 cut(s)	25, 276, 319, 328, 1016, 1154
PspPPI	RGGWCCY	2 cut(s)	1016, 1154
PsuI	RGATCY	1 cut(s)	1330
PvuII	CAGCTG	1 cut(s)	1555
RsaI	GTAC	8 cut(s)	58, 449, 592, 773, 1074, 1306, 1459, 1649
RsaNI	GTAC	8 cut(s)	57, 448, 591, 772, 1073, 1305, 1458, 1648
RseI	CAYNNNNRTG	2 cut(s)	930, 1083
SacI	GAGCTC	2 cut(s)	65, 1623
SaqAI	TTAA	3 cut(s)	1058, 1193, 1274
SatI	GCNGC	8 cut(s)	92, 200, 537, 720, 945, 1176, 1322, 1530
Sau3AI	GATC	5 cut(s)	142, 499, 550, 1330, 1536
Sau96I	GGNCC	6 cut(s)	25, 276, 319, 328, 1016, 1154
ScaI	AGTACT	1 cut(s)	449
SchI	GAGTC	1 cut(s)	688
SduI	GDGCHC	4 cut(s)	65, 180, 1432, 1623
SexAI	ACCWGGT	1 cut(s)	1233
SfaNI	GCATC	2 cut(s)	58, 364
SfuI	TTCGAA	1 cut(s)	1365
SinI	GGWCC	4 cut(s)	319, 328, 1016, 1154
SmaI	CCCGGG	2 cut(s)	466, 854
SmiMI	CAYNNNNRTG	2 cut(s)	930, 1083
SmlI	CTYRAG	1 cut(s)	791
SmoI	CTYRAG	1 cut(s)	791
SpeI	ACTAGT	1 cut(s)	1337
SspI	AATATT	1 cut(s)	729
SspMI	CTAG	4 cut(s)	35, 101, 1020, 1338
SstI	GAGCTC	2 cut(s)	65, 1623
StyI	CCWWGG	3 cut(s)	1019, 1257, 1588
TaiI	ACGT	2 cut(s)	992, 1564
TaqI	TCGA	5 cut(s)	68, 814, 1110, 1365, 1635
TaqII	GACCGA	1 cut(s)	276
TatI	WGTACW	2 cut(s)	447, 1072
TfiI	GAWTC	3 cut(s)	251, 298, 656
Tru1I	TTAA	3 cut(s)	1058, 1193, 1274
Tru9I	TTAA	3 cut(s)	1058, 1193, 1274
TscAI	CASTG	2 cut(s)	937, 1546
TseFI	GTSAC	4 cut(s)	146, 431, 914, 1385
TseI	GCWGC	8 cut(s)	91, 199, 536, 719, 944, 1175, 1321, 1529
Tsp45I	GTSAC	4 cut(s)	146, 431, 914, 1385
TspDTI	ATGAA	5 cut(s)	233, 348, 537, 834, 1244
TspMI	CCCGGG	2 cut(s)	464, 852
TspRI	CASTG	2 cut(s)	937, 1546
VpaK11BI	GGWCC	4 cut(s)	319, 328, 1016, 1154
XagI	CCTNNNNNAGG	2 cut(s)	666, 1023
XapI	RAATTY	4 cut(s)	70, 161, 394, 601
XceI	RCATGY	1 cut(s)	884
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1559
XmaI	CCCGGG	2 cut(s)	464, 852
XmaJI	CCTAGG	1 cut(s)	1019
XmiI	GTMKAC	1 cut(s)	612
XspI	CTAG	4 cut(s)	35, 101, 1020, 1338
ZrmI	AGTACT	1 cut(s)	449

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.