MD04G1133000.v1.1

expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010507
Physical Location & Seq
Reverse (-)
21970980 .. 21972076
1097 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1133000.v1.1.491

Sequence Viewer

Length: 990 bp
ATGGATGATCCTTCCCCCACCAACTCTGTCAATGGCTTCTACTCCTTTCTGGCTCGTGGGATGGACGATCTCGAAAGGGTCTATCTTTCGAACAACTTCATGTCAATCCAATTCCTCCAAAGGTCTCTTACCCTTCTTCGATCTTTCCACTCCCAGCTGACCCTTCTCGTCCAAAAGCTCCATCTGCCTGTTGGGGACAAGTGGCTTGACGAGTACATGGACGAAAGCTCCAAGCTTTGGGAAGCCTGCCATGTCCTCAAGTCCGCCGTCTCCGCCATGGAGAATTATTATACTTCCGGCCTCACCATCACCTCCACTCTCCTCGACTCTCACCACCATCTCAATCCTCAGCTCTCCCGCCAGGTGGTGCGCACGATTTCTCGGTGCCGACGGGAGGCGTTTGGATTGGAGGAAGAGAACAGAGCGCTGATGGAGACGAGAATCCAACCACTCTCACTGAAATTCGACGAAAGGGTCGCCGTCGAATCGAAGCTTAATGGGTTCAGCGGATTCAGGGGAGTCCTGTACGCAATGAGAAACGTGAGCTCTCTGCTTCTCATGATCTTGCTGTACGGACTGGTCTTCTGCTCGCTAGAGGACTCAACCTTTTGCAAGGAAGGAGACTACGACTACGAAGGTTGCTTGATCTTCGGATCACCGTTCATGATATCAACGGCCAGACTGCAGCAGCGGGTGGCGGCGGAGATCGGGCAGATGAGCGAGAGGCCTGGGATTCTGATGTGGGAGTTCAGGCGGTCAAAGGCGGCGATGGAGGAGCTGAGAGGTAAACTGGAAGAGAGGAGGAGCCCACAGCAGGGAGTGATGGATTGGGAGAGTGAGAGTGGGATTGGAGAGAGAGTGGAGAATTTGAGAGAGTGTTTTGGGGTGTTGAATTCTGGTTCTGAGAATATTGTGAGCCAACTTGATGATTTGTTTGATGAAATTGTTGAGGGGAGGAAGAAGCTCTTGGACTTTTGCAGTCATAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BPS1

Protein Analysis

330

Amino Acids

37.74

Weight (kDa)

5.33

Isoelectric Point (pI)

56.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 473
Acc16I TGCGCA 1 cut(s) 371
AccB1I GGYRCC 1 cut(s) 384
AccB7I CCANNNNNTGG 1 cut(s) 237
AciI CCGC 9 cut(s) 264, 273, 358, 507, 691, 698, 701, 754, 764
AclWI GGATC 2 cut(s) 2, 661
AcoI YGGCCR 1 cut(s) 675
AcsI RAATTY 3 cut(s) 461, 865, 892
AfaI GTAC 3 cut(s) 215, 527, 572
AfeI AGCGCT 1 cut(s) 426
AfiI CCNNNNNNNGG 5 cut(s) 237, 364, 394, 814, 815
AgsI TTSAA 1 cut(s) 892
AjnI CCWGG 2 cut(s) 360, 727
AjuI GAANNNNNNNTTGG 2 cut(s) 950, 982
AluBI AGCT 9 cut(s) 157, 178, 228, 235, 352, 493, 546, 778, 964
AluI AGCT 9 cut(s) 157, 178, 228, 235, 352, 493, 546, 778, 964
Alw21I GWGCWC 1 cut(s) 548
Alw26I GTCTC 4 cut(s) 129, 274, 428, 615
AlwI GGATC 2 cut(s) 2, 661
Aor51HI AGCGCT 1 cut(s) 426
AoxI GGCC 3 cut(s) 298, 675, 725
ApeKI GCWGC 2 cut(s) 685, 688
ApoI RAATTY 3 cut(s) 461, 865, 892
Asp700I GAANNNNTTC 1 cut(s) 95
AspLEI GCGC 2 cut(s) 372, 427
AsuHPI GGTGA 4 cut(s) 295, 301, 323, 648
AsuII TTCGAA 1 cut(s) 89
BanI GGYRCC 1 cut(s) 384
BanII GRGCYC 2 cut(s) 548, 809
BauI CACGAG 1 cut(s) 54
BbsI GAAGAC 1 cut(s) 574
Bbv12I GWGCWC 1 cut(s) 548
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 2 cut(s) 697, 700
BccI CCATC 7 cut(s) 55, 189, 314, 345, 424, 763, 817
BceAI ACGGC 3 cut(s) 251, 464, 690
BciT130I CCWGG 2 cut(s) 362, 729
BcoDI GTCTC 4 cut(s) 129, 274, 428, 615
BfaI CTAG 1 cut(s) 593
BfmI CTRYAG 1 cut(s) 683
BfoI RGCGCY 1 cut(s) 428
BisI GCNGC 4 cut(s) 686, 689, 699, 765
BlsI GCNGC 4 cut(s) 687, 690, 700, 766
Bme1390I CCNGG 2 cut(s) 362, 729
BmiI GGNNCC 2 cut(s) 386, 806
BmrFI CCNGG 2 cut(s) 362, 729
BpiI GAAGAC 1 cut(s) 574
Bpu10I CCTNAGC 1 cut(s) 348
Bpu14I TTCGAA 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 242
BsaI GGTCTC 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 276, 728
BsaXI ACNNNNNCTCC 4 cut(s) 212, 242, 843, 873
Bsc4I CCNNNNNNNGG 5 cut(s) 237, 364, 394, 814, 815
Bse1I ACTGG 2 cut(s) 582, 795
Bse3DI GCAATG 1 cut(s) 537
BseBI CCWGG 2 cut(s) 362, 729
BseDI CCNNGG 2 cut(s) 276, 728
BseGI GGATG 2 cut(s) 10, 66
BseLI CCNNNNNNNGG 5 cut(s) 237, 364, 394, 814, 815
BseMI GCAATG 1 cut(s) 537
BseMII CTCAG 3 cut(s) 362, 770, 894
BseNI ACTGG 2 cut(s) 582, 795
BseRI GAGGAG 4 cut(s) 311, 788, 814, 817
BseXI GCAGC 2 cut(s) 697, 700
BseYI CCCAGC 1 cut(s) 153
BshFI GGCC 3 cut(s) 300, 677, 727
BshNI GGYRCC 1 cut(s) 384
BsiHKAI GWGCWC 1 cut(s) 548
BsiSI CCGG 1 cut(s) 297
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 5 cut(s) 237, 364, 394, 814, 815
BsmAI GTCTC 4 cut(s) 129, 274, 428, 615
BsmBI CGTCTC 2 cut(s) 274, 428
BsmFI GGGAC 1 cut(s) 209
BsnI GGCC 3 cut(s) 300, 677, 727
Bso31I GGTCTC 1 cut(s) 129
Bsp119I TTCGAA 1 cut(s) 89
Bsp1286I GDGCHC 2 cut(s) 548, 809
Bsp143I GATC 7 cut(s) 7, 67, 140, 561, 645, 653, 705
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 9 cut(s) 264, 273, 358, 507, 691, 698, 701, 754, 764
BspANI GGCC 3 cut(s) 300, 677, 727
BspCNI CTCAG 3 cut(s) 361, 771, 895
BspHI TCATGA 2 cut(s) 558, 663
BspLI GGNNCC 2 cut(s) 386, 806
BspMAI CTGCAG 1 cut(s) 687
BspPI GGATC 2 cut(s) 2, 661
BspT104I TTCGAA 1 cut(s) 89
BspT107I GGYRCC 1 cut(s) 384
BspTNI GGTCTC 1 cut(s) 129
BsrDI GCAATG 1 cut(s) 537
BsrI ACTGG 2 cut(s) 582, 795
BssECI CCNNGG 2 cut(s) 276, 728
BssMI GATC 7 cut(s) 7, 67, 140, 561, 645, 653, 705
BssSI CACGAG 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 276
Bst2BI CACGAG 1 cut(s) 54
Bst2UI CCWGG 2 cut(s) 362, 729
Bst4CI ACNGT 1 cut(s) 660
Bst6I CTCTTC 2 cut(s) 408, 789
BstBI TTCGAA 1 cut(s) 89
BstC8I GCNNGC 2 cut(s) 247, 590
BstDEI CTNAG 3 cut(s) 348, 779, 903
BstDSI CCRYGG 1 cut(s) 276
BstF5I GGATG 2 cut(s) 10, 66
BstH2I RGCGCY 1 cut(s) 428
BstHHI GCGC 2 cut(s) 372, 427
BstKTI GATC 7 cut(s) 10, 70, 143, 564, 648, 656, 708
BstMAI GTCTC 4 cut(s) 129, 274, 428, 615
BstMBI GATC 7 cut(s) 7, 67, 140, 561, 645, 653, 705
BstMWI GCNNNNNNNGC 2 cut(s) 184, 272
BstNI CCWGG 2 cut(s) 362, 729
BstSCI CCNGG 2 cut(s) 360, 727
BstSFI CTRYAG 1 cut(s) 683
BstV1I GCAGC 2 cut(s) 697, 700
BstV2I GAAGAC 1 cut(s) 574
BsuRI GGCC 3 cut(s) 300, 677, 727
BtgI CCRYGG 1 cut(s) 276
BtgZI GCGATG 1 cut(s) 782
BtsCI GGATG 2 cut(s) 10, 66
BtsIMutI CAGTG 1 cut(s) 455
Cac8I GCNNGC 2 cut(s) 247, 590
CciI TCATGA 2 cut(s) 558, 663
CfoI GCGC 2 cut(s) 372, 427
Csp6I GTAC 3 cut(s) 214, 526, 571
CviAII CATG 6 cut(s) 100, 217, 251, 277, 559, 664
CviQI GTAC 3 cut(s) 214, 526, 571
DdeI CTNAG 3 cut(s) 348, 779, 903
DpnI GATC 7 cut(s) 9, 69, 142, 563, 647, 655, 707
DpnII GATC 7 cut(s) 7, 67, 140, 561, 645, 653, 705
DrdI GACNNNNNNGTC 1 cut(s) 473
DseDI GACNNNNNNGTC 1 cut(s) 473
EaeI YGGCCR 1 cut(s) 675
Eam1104I CTCTTC 2 cut(s) 408, 789
EarI CTCTTC 2 cut(s) 408, 789
EciI GGCGGA 3 cut(s) 253, 262, 716
Ecl136II GAGCTC 1 cut(s) 546
Eco130I CCWWGG 1 cut(s) 276
Eco147I AGGCCT 1 cut(s) 727
Eco24I GRGCYC 2 cut(s) 548, 809
Eco31I GGTCTC 1 cut(s) 129
Eco32I GATATC 1 cut(s) 669
Eco47III AGCGCT 1 cut(s) 426
Eco53kI GAGCTC 1 cut(s) 546
EcoICRI GAGCTC 1 cut(s) 546
EcoRI GAATTC 1 cut(s) 892
EcoRII CCWGG 2 cut(s) 360, 727
EcoRV GATATC 1 cut(s) 669
EcoT14I CCWWGG 1 cut(s) 276
EcoT38I GRGCYC 2 cut(s) 548, 809
ErhI CCWWGG 1 cut(s) 276
Esp3I CGTCTC 2 cut(s) 274, 428
FaeI CATG 6 cut(s) 103, 220, 254, 280, 562, 667
FaiI YATR 8 cut(s) 101, 218, 252, 278, 291, 560, 665, 984
FalI AAGNNNNNCTT 2 cut(s) 950, 982
FaqI GGGAC 1 cut(s) 209
FatI CATG 6 cut(s) 99, 216, 250, 276, 558, 663
FauI CCCGC 2 cut(s) 365, 684
Fnu4HI GCNGC 4 cut(s) 686, 689, 699, 765
FokI GGATG 2 cut(s) 17, 73
FriOI GRGCYC 2 cut(s) 548, 809
Fsp4HI GCNGC 4 cut(s) 686, 689, 699, 765
FspAI RTGCGCAY 1 cut(s) 371
FspBI CTAG 1 cut(s) 593
FspI TGCGCA 1 cut(s) 371
GlaI GCGC 2 cut(s) 371, 426
GluI GCNGC 4 cut(s) 686, 689, 699, 765
GsaI CCCAGC 1 cut(s) 157
HaeII RGCGCY 1 cut(s) 428
HaeIII GGCC 3 cut(s) 300, 677, 727
HapII CCGG 1 cut(s) 297
HhaI GCGC 2 cut(s) 372, 427
Hin1II CATG 6 cut(s) 103, 220, 254, 280, 562, 667
Hin6I GCGC 2 cut(s) 370, 425
HinP1I GCGC 2 cut(s) 370, 425
HindIII AAGCTT 2 cut(s) 233, 491
HinfI GANTC 7 cut(s) 326, 441, 485, 510, 519, 599, 733
HpaII CCGG 1 cut(s) 297
HphI GGTGA 4 cut(s) 295, 301, 323, 648
Hpy166II GTNNAC 1 cut(s) 788
Hpy188I TCNGA 3 cut(s) 653, 738, 904
Hpy188III TCNNGA 3 cut(s) 71, 559, 664
Hpy8I GTNNAC 1 cut(s) 788
Hpy99I CGWCG 3 cut(s) 393, 470, 485
HpyAV CCTTC 5 cut(s) 21, 143, 173, 611, 629
HpyCH4III ACNGT 1 cut(s) 660
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 3 cut(s) 612, 685, 978
HpyF10VI GCNNNNNNNGC 2 cut(s) 184, 272
HpyF3I CTNAG 3 cut(s) 348, 779, 903
HpySE526I ACGT 1 cut(s) 540
Hsp92II CATG 6 cut(s) 103, 220, 254, 280, 562, 667
HspAI GCGC 2 cut(s) 370, 425
Kzo9I GATC 7 cut(s) 7, 67, 140, 561, 645, 653, 705
LmnI GCTCC 4 cut(s) 183, 233, 775, 804
Lsp1109I GCAGC 2 cut(s) 697, 700
MaeI CTAG 1 cut(s) 593
MaeII ACGT 1 cut(s) 540
MalI GATC 7 cut(s) 9, 69, 142, 563, 647, 655, 707
MboI GATC 7 cut(s) 7, 67, 140, 561, 645, 653, 705
MboII GAAGA 6 cut(s) 128, 425, 574, 640, 806, 970
MhlI GDGCHC 2 cut(s) 548, 809
MluCI AATT 6 cut(s) 110, 283, 461, 865, 892, 942
MlyI GAGTC 3 cut(s) 320, 528, 593
MmeI TCCRAC 1 cut(s) 469
MroXI GAANNNNTTC 1 cut(s) 95
MseI TTAA 1 cut(s) 495
MspA1I CMGCKG 3 cut(s) 157, 507, 691
MspI CCGG 1 cut(s) 297
MspR9I CCNGG 2 cut(s) 362, 729
MvaI CCWGG 2 cut(s) 362, 729
MwoI GCNNNNNNNGC 2 cut(s) 184, 272
NcoI CCATGG 1 cut(s) 276
NdeII GATC 7 cut(s) 7, 67, 140, 561, 645, 653, 705
NlaIII CATG 6 cut(s) 103, 220, 254, 280, 562, 667
NlaIV GGNNCC 2 cut(s) 386, 806
NsbI TGCGCA 1 cut(s) 371
NspV TTCGAA 1 cut(s) 89
PagI TCATGA 2 cut(s) 558, 663
PceI AGGCCT 1 cut(s) 727
PdmI GAANNNNTTC 1 cut(s) 95
PfeI GAWTC 4 cut(s) 441, 485, 510, 733
PflMI CCANNNNNTGG 1 cut(s) 237
PkrI GCNGC 4 cut(s) 687, 690, 700, 766
PleI GAGTC 3 cut(s) 320, 527, 593
PpsI GAGTC 3 cut(s) 320, 527, 593
Psp124BI GAGCTC 1 cut(s) 548
Psp6I CCWGG 2 cut(s) 360, 727
PspFI CCCAGC 1 cut(s) 153
PspGI CCWGG 2 cut(s) 360, 727
PspN4I GGNNCC 2 cut(s) 386, 806
PstI CTGCAG 1 cut(s) 687
PvuII CAGCTG 1 cut(s) 157
RsaI GTAC 3 cut(s) 215, 527, 572
RsaNI GTAC 3 cut(s) 214, 526, 571
SacI GAGCTC 1 cut(s) 548
SaqAI TTAA 1 cut(s) 495
SatI GCNGC 4 cut(s) 686, 689, 699, 765
Sau3AI GATC 7 cut(s) 7, 67, 140, 561, 645, 653, 705
SchI GAGTC 3 cut(s) 320, 528, 593
ScrFI CCNGG 2 cut(s) 362, 729
SduI GDGCHC 2 cut(s) 548, 809
SfcI CTRYAG 1 cut(s) 683
SfuI TTCGAA 1 cut(s) 89
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 6 cut(s) 110, 283, 461, 865, 892, 942
SseBI AGGCCT 1 cut(s) 727
SsiI CCGC 9 cut(s) 264, 273, 358, 507, 691, 698, 701, 754, 764
SspI AATATT 1 cut(s) 910
SspMI CTAG 1 cut(s) 593
SstI GAGCTC 1 cut(s) 548
StuI AGGCCT 1 cut(s) 727
StyD4I CCNGG 2 cut(s) 360, 727
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 1 cut(s) 660
TaiI ACGT 1 cut(s) 543
TaqI TCGA 7 cut(s) 72, 89, 139, 324, 465, 483, 488
TasI AATT 6 cut(s) 110, 283, 461, 865, 892, 942
TatI WGTACW 1 cut(s) 213
TauI GCSGC 2 cut(s) 701, 767
TfiI GAWTC 4 cut(s) 441, 485, 510, 733
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TscAI CASTG 1 cut(s) 462
TseI GCWGC 2 cut(s) 685, 688
TspDTI ATGAA 3 cut(s) 88, 652, 954
TspGWI ACGGA 1 cut(s) 588
TspRI CASTG 1 cut(s) 462
Van91I CCANNNNNTGG 1 cut(s) 237
XapI RAATTY 3 cut(s) 461, 865, 892
XcmI CCANNNNNNNNNTGG 1 cut(s) 188
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.