MD08G1173600.v1.1

expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010507
Physical Location & Seq
Reverse (-)
21108987 .. 21110724
1738 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1173600.v1.1.491

Sequence Viewer

Length: 978 bp
ATGGATCCTTCGTCCACCAACTCTGTCAATGGCTTCTATTCGTTTCTGACTCGAGGGATCGACGATCTCGAAAGGGTCTATCTTTCGAACAACTTCATGTCAATCCAATTCCTCCAAAGGGCTCTTTCCCTTATTCGATCTTTCCACTCCCAGCTGACCCTTCTCGTCCAGAAGCTCCACTTGCCCGTCGGGGACAAGTGGCTCGACGAGTACATGGACGAAAGCTCCAAGCTTTGGGAAGCCTGCCATGTCCTTAAGTCCGCCGTCTCCGCTATGGAGAATTACTACACTTCCGGCCTCAACATCACCTCCACCCTCGACTCCCACCGCCATCTCACTCCCCAGCTCTCTCGCCAGGTGGTGCGCGCGATTTCTGGGTGTCGGCGAGAGGCGTTCGGATTGGAGGAGGAGAACAGGGGGCTGATGGAAACGAGAATCCAGCCGCTGTCGCTCAAATTCGACGAAAGGGTCTCCATCGAATCGAAGCTCAATGGGTTCAATGGGTTCAGGGGAGTCCTGTACGCAATGAGGAACGTGAGCTCGCTGCTACTCATGATCTTGCTGTACGGACTGGTGTTCTGCTTGCCGGAGGAGGAGTCGAGCTTCTGCAGGGGAGGGTACAACGAGGGGTGCATGATCTTCGGATCATCGTTCATGATATCGACGGCGAGGCTGCAGCAGAGGGTGGCGGCGGAGATCGGGCAGATCAGAGAGAGGCCGGGGGTTCTGATGTGGGAGTTCAGGAGGTCGAAGGGGGCGATGGAGGAGCTGAGGGGGGAGCTGGAGAGGGGGGGCCCACAGGGGGTGGTGGAGTGGGAGGTTGACGGCGGCGGGATTAGGGAGGGAGTGGACAACTTGAAAGCGTGTTTTGGGGTGCTGAGATCTGGTGCGGAGAATATTGCGAGCCAACTTGATGATTTCATTGATGAAATTGTTGAGGGGAGGAAGAAGCTTTTGGACTTTTGCAGTCATAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BPS1

Protein Analysis

326

Amino Acids

36.78

Weight (kDa)

5.66

Isoelectric Point (pI)

47.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 467
AccB7I CCANNNNNTGG 1 cut(s) 234
AccII CGCG 2 cut(s) 366, 368
AciI CCGC 9 cut(s) 261, 270, 328, 443, 689, 692, 828, 831, 890
AclWI GGATC 3 cut(s) 12, 65, 652
AcsI RAATTY 1 cut(s) 455
AfaI GTAC 4 cut(s) 212, 521, 566, 620
AfiI CCNNNNNNNGG 3 cut(s) 118, 234, 802
AflII CTTAAG 1 cut(s) 254
AgsI TTSAA 2 cut(s) 499, 859
AjnI CCWGG 1 cut(s) 354
AjuI GAANNNNNNNTTGG 2 cut(s) 938, 970
Alw21I GWGCWC 1 cut(s) 542
Alw26I GTCTC 2 cut(s) 271, 475
AlwI GGATC 3 cut(s) 12, 65, 652
AlwNI CAGNNNCTG 1 cut(s) 445
Ama87I CYCGRG 1 cut(s) 51
AoxI GGCC 3 cut(s) 295, 716, 793
ApaI GGGCCC 1 cut(s) 797
ApeKI GCWGC 3 cut(s) 544, 673, 676
ApoI RAATTY 1 cut(s) 455
Asp700I GAANNNNTTC 1 cut(s) 92
AspLEI GCGC 2 cut(s) 366, 368
AspS9I GGNCC 2 cut(s) 793, 794
AsuC2I CCSGG 1 cut(s) 720
AsuHPI GGTGA 1 cut(s) 298
AsuII TTCGAA 1 cut(s) 86
AvaI CYCGRG 1 cut(s) 51
BaeGI GKGCMC 1 cut(s) 797
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 3 cut(s) 124, 542, 797
Bbv12I GWGCWC 1 cut(s) 542
BbvCI CCTCAGC 1 cut(s) 770
BbvI GCAGC 3 cut(s) 531, 660, 688
BccI CCATC 4 cut(s) 339, 418, 482, 754
BceAI ACGGC 3 cut(s) 248, 681, 841
BcgI CGANNNNNNTGC 2 cut(s) 622, 656
BciT130I CCWGG 1 cut(s) 356
BcnI CCSGG 1 cut(s) 720
BcoDI GTCTC 2 cut(s) 271, 475
BfmI CTRYAG 2 cut(s) 607, 674
BfrI CTTAAG 1 cut(s) 254
BglII AGATCT 1 cut(s) 881
BisI GCNGC 6 cut(s) 443, 545, 674, 677, 690, 829
BlsI GCNGC 6 cut(s) 444, 546, 675, 678, 691, 830
Bme1390I CCNGG 2 cut(s) 356, 720
BmeT110I CYCGRG 1 cut(s) 51
BmgT120I GGNCC 2 cut(s) 793, 794
BmiI GGNNCC 3 cut(s) 6, 794, 795
BmrFI CCNGG 2 cut(s) 356, 720
BpmI CTGGAG 1 cut(s) 803
Bpu10I CCTNAGC 1 cut(s) 770
Bpu14I TTCGAA 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 720
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 1 cut(s) 719
BsaXI ACNNNNNCTCC 6 cut(s) 209, 239, 293, 323, 581, 611
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 234, 802
Bse1I ACTGG 1 cut(s) 576
Bse3DI GCAATG 1 cut(s) 531
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 1 cut(s) 719
BseLI CCNNNNNNNGG 3 cut(s) 118, 234, 802
BseMI GCAATG 1 cut(s) 531
BseMII CTCAG 2 cut(s) 761, 869
BseNI ACTGG 1 cut(s) 576
BsePI GCGCGC 1 cut(s) 364
BseRI GAGGAG 5 cut(s) 419, 422, 605, 608, 779
BseSI GKGCMC 1 cut(s) 797
BseXI GCAGC 3 cut(s) 531, 660, 688
BseYI CCCAGC 2 cut(s) 150, 342
Bsh1236I CGCG 2 cut(s) 366, 368
BshFI GGCC 3 cut(s) 297, 718, 795
BsiHKAI GWGCWC 1 cut(s) 542
BsiHKCI CYCGRG 1 cut(s) 51
BsiSI CCGG 3 cut(s) 294, 587, 719
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 3 cut(s) 118, 234, 802
BsmAI GTCTC 2 cut(s) 271, 475
BsmBI CGTCTC 1 cut(s) 271
BsmFI GGGAC 1 cut(s) 206
BsnI GGCC 3 cut(s) 297, 718, 795
Bso31I GGTCTC 1 cut(s) 475
BsoBI CYCGRG 1 cut(s) 51
Bsp119I TTCGAA 1 cut(s) 86
Bsp120I GGGCCC 1 cut(s) 793
Bsp1286I GDGCHC 3 cut(s) 124, 542, 797
BspACI CCGC 9 cut(s) 261, 270, 328, 443, 689, 692, 828, 831, 890
BspANI GGCC 3 cut(s) 297, 718, 795
BspCNI CTCAG 2 cut(s) 762, 870
BspFNI CGCG 2 cut(s) 366, 368
BspHI TCATGA 2 cut(s) 552, 654
BspLI GGNNCC 3 cut(s) 6, 794, 795
BspMAI CTGCAG 2 cut(s) 611, 678
BspPI GGATC 3 cut(s) 12, 65, 652
BspT104I TTCGAA 1 cut(s) 86
BspTI CTTAAG 1 cut(s) 254
BspTNI GGTCTC 1 cut(s) 475
BsrDI GCAATG 1 cut(s) 531
BsrI ACTGG 1 cut(s) 576
BssECI CCNNGG 1 cut(s) 719
BssHII GCGCGC 1 cut(s) 364
Bst2UI CCWGG 1 cut(s) 356
BstAFI CTTAAG 1 cut(s) 254
BstBI TTCGAA 1 cut(s) 86
BstC8I GCNNGC 5 cut(s) 244, 366, 542, 584, 904
BstDEI CTNAG 2 cut(s) 770, 878
BstFNI CGCG 2 cut(s) 366, 368
BstHHI GCGC 2 cut(s) 366, 368
BstMAI GTCTC 2 cut(s) 271, 475
BstMWI GCNNNNNNNGC 3 cut(s) 181, 269, 448
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 2 cut(s) 354, 718
BstSFI CTRYAG 2 cut(s) 607, 674
BstSLI GKGCMC 1 cut(s) 797
BstUI CGCG 2 cut(s) 366, 368
BstV1I GCAGC 3 cut(s) 531, 660, 688
BstX2I RGATCY 2 cut(s) 4, 881
BstYI RGATCY 2 cut(s) 4, 881
BsuRI GGCC 3 cut(s) 297, 718, 795
BtgZI GCGATG 1 cut(s) 773
Cac8I GCNNGC 5 cut(s) 244, 366, 542, 584, 904
CaiI CAGNNNCTG 1 cut(s) 445
CciI TCATGA 2 cut(s) 552, 654
CfoI GCGC 2 cut(s) 366, 368
Cfr13I GGNCC 2 cut(s) 793, 794
Csp6I GTAC 4 cut(s) 211, 520, 565, 619
CviAII CATG 6 cut(s) 97, 214, 248, 553, 634, 655
CviQI GTAC 4 cut(s) 211, 520, 565, 619
DdeI CTNAG 2 cut(s) 770, 878
DrdI GACNNNNNNGTC 1 cut(s) 467
DseDI GACNNNNNNGTC 1 cut(s) 467
EciI GGCGGA 2 cut(s) 250, 707
Ecl136II GAGCTC 1 cut(s) 540
Eco24I GRGCYC 3 cut(s) 124, 542, 797
Eco31I GGTCTC 1 cut(s) 475
Eco32I GATATC 1 cut(s) 660
Eco53kI GAGCTC 1 cut(s) 540
Eco88I CYCGRG 1 cut(s) 51
EcoICRI GAGCTC 1 cut(s) 540
EcoO109I RGGNCCY 1 cut(s) 793
EcoRII CCWGG 1 cut(s) 354
EcoRV GATATC 1 cut(s) 660
EcoT38I GRGCYC 3 cut(s) 124, 542, 797
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 6 cut(s) 100, 217, 251, 556, 637, 658
FaiI YATR 8 cut(s) 98, 215, 249, 275, 554, 635, 656, 972
FalI AAGNNNNNCTT 2 cut(s) 164, 196
FaqI GGGAC 1 cut(s) 206
FatI CATG 6 cut(s) 96, 213, 247, 552, 633, 654
FauI CCCGC 1 cut(s) 824
Fnu4HI GCNGC 6 cut(s) 443, 545, 674, 677, 690, 829
FriOI GRGCYC 3 cut(s) 124, 542, 797
Fsp4HI GCNGC 6 cut(s) 443, 545, 674, 677, 690, 829
GlaI GCGC 2 cut(s) 365, 367
GluI GCNGC 6 cut(s) 443, 545, 674, 677, 690, 829
GsaI CCCAGC 2 cut(s) 154, 346
GsuI CTGGAG 1 cut(s) 803
HaeIII GGCC 3 cut(s) 297, 718, 795
HapII CCGG 3 cut(s) 294, 587, 719
HhaI GCGC 2 cut(s) 366, 368
Hin1II CATG 6 cut(s) 100, 217, 251, 556, 637, 658
Hin6I GCGC 2 cut(s) 364, 366
HinP1I GCGC 2 cut(s) 364, 366
HincII GTYRAC 1 cut(s) 823
HindII GTYRAC 1 cut(s) 823
HindIII AAGCTT 2 cut(s) 230, 950
HinfI GANTC 6 cut(s) 49, 320, 435, 479, 513, 596
HpaII CCGG 3 cut(s) 294, 587, 719
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 3 cut(s) 15, 823, 850
Hpy188I TCNGA 5 cut(s) 48, 398, 644, 710, 729
Hpy188III TCNNGA 5 cut(s) 68, 169, 553, 655, 742
Hpy8I GTNNAC 3 cut(s) 15, 823, 850
Hpy99I CGWCG 5 cut(s) 65, 191, 209, 464, 667
HpyAV CCTTC 3 cut(s) 18, 170, 745
HpyCH4IV ACGT 1 cut(s) 534
HpyCH4V TGCA 4 cut(s) 609, 633, 676, 966
HpyF10VI GCNNNNNNNGC 3 cut(s) 181, 269, 448
HpyF3I CTNAG 2 cut(s) 770, 878
HpySE526I ACGT 1 cut(s) 534
Hsp92II CATG 6 cut(s) 100, 217, 251, 556, 637, 658
HspAI GCGC 2 cut(s) 364, 366
LmnI GCTCC 4 cut(s) 180, 230, 766, 778
Lsp1109I GCAGC 3 cut(s) 531, 660, 688
MaeII ACGT 1 cut(s) 534
MboII GAAGA 2 cut(s) 631, 958
MflI RGATCY 2 cut(s) 4, 881
MhlI GDGCHC 3 cut(s) 124, 542, 797
MluCI AATT 4 cut(s) 107, 280, 455, 930
MlyI GAGTC 4 cut(s) 43, 314, 522, 605
MroXI GAANNNNTTC 1 cut(s) 92
MseI TTAA 1 cut(s) 255
MspA1I CMGCKG 2 cut(s) 154, 445
MspCI CTTAAG 1 cut(s) 254
MspI CCGG 3 cut(s) 294, 587, 719
MspR9I CCNGG 2 cut(s) 356, 720
MvaI CCWGG 1 cut(s) 356
MvnI CGCG 2 cut(s) 366, 368
MwoI GCNNNNNNNGC 3 cut(s) 181, 269, 448
NciI CCSGG 1 cut(s) 720
NlaIII CATG 6 cut(s) 100, 217, 251, 556, 637, 658
NlaIV GGNNCC 3 cut(s) 6, 794, 795
NspV TTCGAA 1 cut(s) 86
PaeR7I CTCGAG 1 cut(s) 51
PagI TCATGA 2 cut(s) 552, 654
PauI GCGCGC 1 cut(s) 364
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 755
PdmI GAANNNNTTC 1 cut(s) 92
PfeI GAWTC 2 cut(s) 435, 479
PflMI CCANNNNNTGG 1 cut(s) 234
PkrI GCNGC 6 cut(s) 444, 546, 675, 678, 691, 830
PleI GAGTC 4 cut(s) 43, 314, 521, 604
PpsI GAGTC 4 cut(s) 43, 314, 521, 604
Psp124BI GAGCTC 1 cut(s) 542
Psp6I CCWGG 1 cut(s) 354
PspFI CCCAGC 2 cut(s) 150, 342
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 3 cut(s) 6, 794, 795
PspOMI GGGCCC 1 cut(s) 793
PspPI GGNCC 2 cut(s) 793, 794
PspXI VCTCGAGB 1 cut(s) 51
PstI CTGCAG 2 cut(s) 611, 678
PstNI CAGNNNCTG 1 cut(s) 445
PsuI RGATCY 2 cut(s) 4, 881
PteI GCGCGC 1 cut(s) 364
PvuII CAGCTG 1 cut(s) 154
RsaI GTAC 4 cut(s) 212, 521, 566, 620
RsaNI GTAC 4 cut(s) 211, 520, 565, 619
SacI GAGCTC 1 cut(s) 542
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 6 cut(s) 443, 545, 674, 677, 690, 829
Sau96I GGNCC 2 cut(s) 793, 794
SchI GAGTC 4 cut(s) 43, 314, 522, 605
ScrFI CCNGG 2 cut(s) 356, 720
SduI GDGCHC 3 cut(s) 124, 542, 797
SfcI CTRYAG 2 cut(s) 607, 674
Sfr274I CTCGAG 1 cut(s) 51
SfuI TTCGAA 1 cut(s) 86
SlaI CTCGAG 1 cut(s) 51
SmlI CTYRAG 2 cut(s) 51, 254
SmoI CTYRAG 2 cut(s) 51, 254
Sse9I AATT 4 cut(s) 107, 280, 455, 930
SsiI CCGC 9 cut(s) 261, 270, 328, 443, 689, 692, 828, 831, 890
SspI AATATT 1 cut(s) 898
SstI GAGCTC 1 cut(s) 542
StyD4I CCNGG 2 cut(s) 354, 718
TaiI ACGT 1 cut(s) 537
TasI AATT 4 cut(s) 107, 280, 455, 930
TatI WGTACW 1 cut(s) 210
TauI GCSGC 3 cut(s) 445, 692, 831
TfiI GAWTC 2 cut(s) 435, 479
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TseI GCWGC 3 cut(s) 544, 673, 676
TspDTI ATGAA 4 cut(s) 85, 643, 910, 942
TspGWI ACGGA 1 cut(s) 582
Van91I CCANNNNNTGG 1 cut(s) 234
Vha464I CTTAAG 1 cut(s) 254
XapI RAATTY 1 cut(s) 455
XhoI CTCGAG 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.