pycom15g32220

expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010507
Physical Location & Seq
Reverse (-)
31119301 .. 31120364
1064 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g32220.2

Sequence Viewer

Length: 978 bp
ATGGATCGATCATCTACCAACTCTGTCAATGGCTTCTACTCGTTTCTGACTCGAGGGATCGACGATCTCGAAAGGGTTTTTCTTTCCAACAACTTTATGTCAATCCAATTCCTCCAAAGGGCTCTTTCCCTTCTTCGATCTTTCCACTCCCAGCTGACCCTTCTCGTCCAGAAGCTCCATTTGCCCGTCGGAGACAAGTGGCTCGACGAGTACATGGACGAAAGCTCCAAGCTTTGGGAAGCCTGCCATGTCCTCAAGTCCGCCGTCTCCGCCATGGAGAATTACCACACTTCCGGCCTCAACATCACCTCCACCCTCGACTCCCACCGCCATCTCACTCCCCAGCTCTCTCGCCAGGTGGTGCGCGCGATTTCTGGGTGTCGGCGACAGGCGTTCGGATTGGAGGAAGAGAACAGGGGGCTGATGGAGACGAGAATCCAGCCGCTGTCGCTCAAATTCGACGAAAGCGTCTCCATCGAATCGAAGCTCAATGGATTTAATGGGTTCAGGGGAGTCCTGTACGCAATGAGGAACGTGAGCTCGCTGCTGCTAATTATCCTGCTGTACGGACTGGTGTTCTGCTGGCCGGAGGAGGAGTCGAGCTTCTGCAGGGGAGGGTACAACGAGGGGTGCGTGATCTTCGGATCGTCGTTCATGGTATCGACGGCGAGGCTGCAGCAGAGGGTTGCGGCGGAGATCAGGCAGATCAGGGAGAGGCCGGGGGTTCTGATGTGGGAGTTCAGGAGGTCGAAGGCGGCGATGGAGGAGCTGAGGGGGGATCTCGAGAGGAGGGGGCCACAGGGAGTGGTGGAGTGGGAGGAGGAGGGCGGCGGGATTAGGGAGGGAGTGGACAACTTGAGAGAGTGTTTTGGGGTGTTGAGATCCGGTGCGGAGAATATTGTGAACCAACTTGATGATTTCTTTGATGAAATTGTTGAGGGGAGGAAGAAGCTTTTGGACTTTTGCGGTCATAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BPS1

Protein Analysis

326

Amino Acids

37.12

Weight (kDa)

5.9

Isoelectric Point (pI)

53.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 467
AccB7I CCANNNNNTGG 1 cut(s) 234
AccII CGCG 2 cut(s) 366, 368
AclWI GGATC 5 cut(s) 12, 65, 652, 786, 876
AcoI YGGCCR 1 cut(s) 584
AcsI RAATTY 1 cut(s) 455
AfaI GTAC 4 cut(s) 212, 521, 566, 620
AfiI CCNNNNNNNGG 2 cut(s) 118, 234
AjnI CCWGG 1 cut(s) 354
AjuI GAANNNNNNNTTGG 2 cut(s) 938, 970
Alw21I GWGCWC 1 cut(s) 542
Alw26I GTCTC 4 cut(s) 186, 271, 422, 475
AlwI GGATC 5 cut(s) 12, 65, 652, 786, 876
AlwNI CAGNNNCTG 1 cut(s) 445
Ama87I CYCGRG 2 cut(s) 51, 782
AoxI GGCC 4 cut(s) 295, 584, 716, 794
ApeKI GCWGC 4 cut(s) 544, 547, 673, 676
ApoI RAATTY 1 cut(s) 455
AspLEI GCGC 2 cut(s) 366, 368
AspS9I GGNCC 1 cut(s) 794
AsuC2I CCSGG 1 cut(s) 720
AsuHPI GGTGA 1 cut(s) 298
AvaI CYCGRG 2 cut(s) 51, 782
BanII GRGCYC 2 cut(s) 124, 542
Bbv12I GWGCWC 1 cut(s) 542
BbvCI CCTCAGC 1 cut(s) 770
BbvI GCAGC 4 cut(s) 531, 534, 660, 688
BccI CCATC 4 cut(s) 339, 418, 482, 754
BceAI ACGGC 2 cut(s) 248, 681
BciT130I CCWGG 1 cut(s) 356
BcnI CCSGG 1 cut(s) 720
BcoDI GTCTC 4 cut(s) 186, 271, 422, 475
BfmI CTRYAG 2 cut(s) 607, 674
BisI GCNGC 8 cut(s) 443, 545, 548, 674, 677, 690, 756, 829
BlsI GCNGC 8 cut(s) 444, 546, 549, 675, 678, 691, 757, 830
Bme1390I CCNGG 2 cut(s) 356, 720
BmeT110I CYCGRG 2 cut(s) 51, 782
BmgT120I GGNCC 1 cut(s) 794
BmiI GGNNCC 1 cut(s) 795
BmrFI CCNGG 2 cut(s) 356, 720
Bpu10I CCTNAGC 1 cut(s) 770
BpuEI CTTGAG 2 cut(s) 239, 877
BpuMI CCSGG 1 cut(s) 720
Bsa29I ATCGAT 1 cut(s) 7
BsaJI CCNNGG 2 cut(s) 273, 719
BsaWI WCCGGW 1 cut(s) 884
BsaXI ACNNNNNCTCC 6 cut(s) 209, 239, 293, 323, 581, 611
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 234
Bse1I ACTGG 1 cut(s) 576
Bse3DI GCAATG 1 cut(s) 531
BseBI CCWGG 1 cut(s) 356
BseCI ATCGAT 1 cut(s) 7
BseDI CCNNGG 2 cut(s) 273, 719
BseLI CCNNNNNNNGG 2 cut(s) 118, 234
BseMI GCAATG 1 cut(s) 531
BseMII CTCAG 1 cut(s) 761
BseNI ACTGG 1 cut(s) 576
BsePI GCGCGC 1 cut(s) 364
BseRI GAGGAG 6 cut(s) 605, 608, 779, 802, 833, 836
BseXI GCAGC 4 cut(s) 531, 534, 660, 688
BseYI CCCAGC 2 cut(s) 150, 342
Bsh1236I CGCG 2 cut(s) 366, 368
BshFI GGCC 4 cut(s) 297, 586, 718, 796
BshVI ATCGAT 1 cut(s) 7
BsiHKAI GWGCWC 1 cut(s) 542
BsiHKCI CYCGRG 2 cut(s) 51, 782
BsiSI CCGG 4 cut(s) 294, 587, 719, 885
BslI CCNNNNNNNGG 2 cut(s) 118, 234
BsmAI GTCTC 4 cut(s) 186, 271, 422, 475
BsmBI CGTCTC 3 cut(s) 271, 422, 475
BsnI GGCC 4 cut(s) 297, 586, 718, 796
BsoBI CYCGRG 2 cut(s) 51, 782
Bsp1286I GDGCHC 2 cut(s) 124, 542
Bsp19I CCATGG 1 cut(s) 273
BspANI GGCC 4 cut(s) 297, 586, 718, 796
BspCNI CTCAG 1 cut(s) 762
BspDI ATCGAT 1 cut(s) 7
BspFNI CGCG 2 cut(s) 366, 368
BspLI GGNNCC 1 cut(s) 795
BspMAI CTGCAG 2 cut(s) 611, 678
BspPI GGATC 5 cut(s) 12, 65, 652, 786, 876
BsrDI GCAATG 1 cut(s) 531
BsrI ACTGG 1 cut(s) 576
BssECI CCNNGG 2 cut(s) 273, 719
BssHII GCGCGC 1 cut(s) 364
BssT1I CCWWGG 1 cut(s) 273
Bst2UI CCWGG 1 cut(s) 356
Bst6I CTCTTC 1 cut(s) 402
BstC8I GCNNGC 4 cut(s) 244, 366, 542, 584
BstDEI CTNAG 1 cut(s) 770
BstDSI CCRYGG 1 cut(s) 273
BstFNI CGCG 2 cut(s) 366, 368
BstHHI GCGC 2 cut(s) 366, 368
BstMAI GTCTC 4 cut(s) 186, 271, 422, 475
BstMWI GCNNNNNNNGC 3 cut(s) 181, 269, 448
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 2 cut(s) 354, 718
BstSFI CTRYAG 2 cut(s) 607, 674
BstUI CGCG 2 cut(s) 366, 368
BstV1I GCAGC 4 cut(s) 531, 534, 660, 688
BstX2I RGATCY 2 cut(s) 778, 881
BstYI RGATCY 2 cut(s) 778, 881
Bsu15I ATCGAT 1 cut(s) 7
BsuRI GGCC 4 cut(s) 297, 586, 718, 796
BsuTUI ATCGAT 1 cut(s) 7
BtgI CCRYGG 1 cut(s) 273
BtgZI GCGATG 1 cut(s) 773
Cac8I GCNNGC 4 cut(s) 244, 366, 542, 584
CaiI CAGNNNCTG 1 cut(s) 445
CfoI GCGC 2 cut(s) 366, 368
Cfr13I GGNCC 1 cut(s) 794
ClaI ATCGAT 1 cut(s) 7
CseI GACGC 1 cut(s) 457
Csp6I GTAC 4 cut(s) 211, 520, 565, 619
CviAII CATG 4 cut(s) 214, 248, 274, 655
CviQI GTAC 4 cut(s) 211, 520, 565, 619
DdeI CTNAG 1 cut(s) 770
DrdI GACNNNNNNGTC 1 cut(s) 467
DseDI GACNNNNNNGTC 1 cut(s) 467
EaeI YGGCCR 1 cut(s) 584
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
EciI GGCGGA 3 cut(s) 250, 259, 707
Ecl136II GAGCTC 1 cut(s) 540
Eco130I CCWWGG 1 cut(s) 273
Eco24I GRGCYC 2 cut(s) 124, 542
Eco53kI GAGCTC 1 cut(s) 540
Eco88I CYCGRG 2 cut(s) 51, 782
EcoICRI GAGCTC 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 273
EcoT38I GRGCYC 2 cut(s) 124, 542
ErhI CCWWGG 1 cut(s) 273
Esp3I CGTCTC 3 cut(s) 271, 422, 475
FaeI CATG 4 cut(s) 217, 251, 277, 658
FaiI YATR 6 cut(s) 98, 215, 249, 275, 656, 972
FatI CATG 4 cut(s) 213, 247, 273, 654
FauI CCCGC 1 cut(s) 824
Fnu4HI GCNGC 8 cut(s) 443, 545, 548, 674, 677, 690, 756, 829
FriOI GRGCYC 2 cut(s) 124, 542
Fsp4HI GCNGC 8 cut(s) 443, 545, 548, 674, 677, 690, 756, 829
GlaI GCGC 2 cut(s) 365, 367
GluI GCNGC 8 cut(s) 443, 545, 548, 674, 677, 690, 756, 829
GsaI CCCAGC 2 cut(s) 154, 346
HaeIII GGCC 4 cut(s) 297, 586, 718, 796
HapII CCGG 4 cut(s) 294, 587, 719, 885
HgaI GACGC 1 cut(s) 457
HhaI GCGC 2 cut(s) 366, 368
Hin1II CATG 4 cut(s) 217, 251, 277, 658
Hin6I GCGC 2 cut(s) 364, 366
HinP1I GCGC 2 cut(s) 364, 366
HindIII AAGCTT 2 cut(s) 230, 950
HinfI GANTC 6 cut(s) 49, 320, 435, 479, 513, 596
HpaII CCGG 4 cut(s) 294, 587, 719, 885
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 2 cut(s) 850, 904
Hpy188I TCNGA 5 cut(s) 48, 191, 398, 644, 729
Hpy188III TCNNGA 5 cut(s) 68, 169, 742, 782, 784
Hpy8I GTNNAC 2 cut(s) 850, 904
Hpy99I CGWCG 6 cut(s) 65, 191, 209, 464, 652, 667
HpyAV CCTTC 3 cut(s) 140, 170, 745
HpyCH4IV ACGT 1 cut(s) 534
HpyCH4V TGCA 2 cut(s) 609, 676
HpyF10VI GCNNNNNNNGC 3 cut(s) 181, 269, 448
HpyF3I CTNAG 1 cut(s) 770
HpySE526I ACGT 1 cut(s) 534
Hsp92II CATG 4 cut(s) 217, 251, 277, 658
HspAI GCGC 2 cut(s) 364, 366
LmnI GCTCC 3 cut(s) 180, 230, 766
Lsp1109I GCAGC 4 cut(s) 531, 534, 660, 688
MaeII ACGT 1 cut(s) 534
MboII GAAGA 4 cut(s) 125, 419, 631, 958
MflI RGATCY 2 cut(s) 778, 881
MhlI GDGCHC 2 cut(s) 124, 542
MluCI AATT 5 cut(s) 107, 280, 455, 552, 930
MlyI GAGTC 4 cut(s) 43, 314, 522, 605
MmeI TCCRAC 2 cut(s) 111, 169
MseI TTAA 1 cut(s) 498
MspA1I CMGCKG 2 cut(s) 154, 445
MspI CCGG 4 cut(s) 294, 587, 719, 885
MspR9I CCNGG 2 cut(s) 356, 720
MvaI CCWGG 1 cut(s) 356
MvnI CGCG 2 cut(s) 366, 368
MwoI GCNNNNNNNGC 3 cut(s) 181, 269, 448
NciI CCSGG 1 cut(s) 720
NcoI CCATGG 1 cut(s) 273
NlaIII CATG 4 cut(s) 217, 251, 277, 658
NlaIV GGNNCC 1 cut(s) 795
PaeR7I CTCGAG 2 cut(s) 51, 782
PauI GCGCGC 1 cut(s) 364
PcsI WCGNNNNNNNCGW 4 cut(s) 66, 465, 630, 755
PfeI GAWTC 2 cut(s) 435, 479
PflMI CCANNNNNTGG 1 cut(s) 234
PkrI GCNGC 8 cut(s) 444, 546, 549, 675, 678, 691, 757, 830
PleI GAGTC 4 cut(s) 43, 314, 521, 604
PpsI GAGTC 4 cut(s) 43, 314, 521, 604
Psp124BI GAGCTC 1 cut(s) 542
Psp6I CCWGG 1 cut(s) 354
PspFI CCCAGC 2 cut(s) 150, 342
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 795
PspPI GGNCC 1 cut(s) 794
PspXI VCTCGAGB 1 cut(s) 51
PstI CTGCAG 2 cut(s) 611, 678
PstNI CAGNNNCTG 1 cut(s) 445
PsuI RGATCY 2 cut(s) 778, 881
PteI GCGCGC 1 cut(s) 364
PvuII CAGCTG 1 cut(s) 154
RsaI GTAC 4 cut(s) 212, 521, 566, 620
RsaNI GTAC 4 cut(s) 211, 520, 565, 619
SacI GAGCTC 1 cut(s) 542
SaqAI TTAA 1 cut(s) 498
SatI GCNGC 8 cut(s) 443, 545, 548, 674, 677, 690, 756, 829
Sau96I GGNCC 1 cut(s) 794
SchI GAGTC 4 cut(s) 43, 314, 522, 605
ScrFI CCNGG 2 cut(s) 356, 720
SduI GDGCHC 2 cut(s) 124, 542
SfcI CTRYAG 2 cut(s) 607, 674
Sfr274I CTCGAG 2 cut(s) 51, 782
SlaI CTCGAG 2 cut(s) 51, 782
SmlI CTYRAG 4 cut(s) 51, 254, 782, 856
SmoI CTYRAG 4 cut(s) 51, 254, 782, 856
Sse9I AATT 5 cut(s) 107, 280, 455, 552, 930
SspI AATATT 1 cut(s) 898
SstI GAGCTC 1 cut(s) 542
StyD4I CCNGG 2 cut(s) 354, 718
StyI CCWWGG 1 cut(s) 273
TaiI ACGT 1 cut(s) 537
TasI AATT 5 cut(s) 107, 280, 455, 552, 930
TatI WGTACW 1 cut(s) 210
TauI GCSGC 4 cut(s) 445, 692, 758, 831
TfiI GAWTC 2 cut(s) 435, 479
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TseI GCWGC 4 cut(s) 544, 547, 673, 676
TspDTI ATGAA 2 cut(s) 643, 942
TspGWI ACGGA 1 cut(s) 582
Van91I CCANNNNNTGG 1 cut(s) 234
XapI RAATTY 1 cut(s) 455
XhoI CTCGAG 2 cut(s) 51, 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.