pycom08g14900

expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010507
Physical Location & Seq
Reverse (-)
14317513 .. 14318573
1061 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g14900.2

Sequence Viewer

Length: 975 bp
ATGGATCGTTCATCCACCAACTCTGTCAATGGCTTCTATTCGTTTCTGACTCGAGGGATCGACGATCTCGAAAGGGTCTATCTTTCGAACAACTTCATGTCAATCCAATTCCTCCAAAGGGCTCTTTCCCTTCTTCGATCTTTCCACTCCCAGCTGGCCCTTCTCGTCCAGAAGCTCCATTTGCCCGTCGGGGACAAGTGGCTCGACGAGTACATGGGCGAAAGCTCCAAGCTTTGGGACGCCTGCCATGTCCTCAAGTCCGCCGTCTCCGCTATGGAGAATTACTACACTTCCGGCCTTAACATCACCTCCACCTTCGACTCCCACCGCCATCTCACTCCCCAGCTCTCTCGCCAGGTGGTGCGCGCGATTTCTGGCTGTCGGCGAGAGGCGTTCGGATTGGAAGAGGAGAACAGGGGGCTGATGGAAACGAGAATCCAGCCGCTGTCGCTCAAATTCGACGAAGGGGTCTCCATCGAATCGAAGCTCAACGGGTTCAATGGGTTCAGGGGAGTCCTGTACGCAATGAGGAACGTGAGCTCGCTGCTACTCATGATCTTGCTGTACGGACTGGTGTTCTGCTTGCCGGAGGAGTCGAGCTTCTGCAGGGGAGGGTACAACGAGGGGTGCATGATCTTCGGATCATCGTTCATGATATCGACGGCGAGGCTGCAGCAGAGGGTGGCGGCGGAGATCGGGCAGATCAGAGAGAGGCCGGGGGTTCTGATGTGGGAGTTCAGGAGGTCGAAGGCGGCGATGGAGGAGCTGAGGGGGGAGCTGGAGAGGGGGGGCCAACAGGGGGTGGTGGAGTGGGAGGTTGACGGCGGCGGGATTAGGGAGGGAGTGGACAACTTGAAAGCGAGTTTTGGGGTGCTGAGATCTGGTGCGGAGAATATTGCGAGCCAACTTGATGATTTCTTTGATGAAATTGTTGAGGGGAGGAAGAAGCTTTTGGACTTTTGCAGTCATAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BPS1

Protein Analysis

325

Amino Acids

36.6

Weight (kDa)

5.95

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 467
AccB7I CCANNNNNTGG 1 cut(s) 234
AccII CGCG 2 cut(s) 366, 368
AclWI GGATC 3 cut(s) 12, 65, 649
AcsI RAATTY 1 cut(s) 455
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 4 cut(s) 212, 521, 566, 617
AfiI CCNNNNNNNGG 3 cut(s) 118, 234, 799
AgsI TTSAA 2 cut(s) 499, 856
AjnI CCWGG 1 cut(s) 354
AjuI GAANNNNNNNTTGG 2 cut(s) 935, 967
Alw21I GWGCWC 1 cut(s) 542
Alw26I GTCTC 2 cut(s) 271, 475
AlwI GGATC 3 cut(s) 12, 65, 649
AlwNI CAGNNNCTG 1 cut(s) 445
Ama87I CYCGRG 1 cut(s) 51
AoxI GGCC 4 cut(s) 156, 295, 713, 790
ApeKI GCWGC 3 cut(s) 544, 670, 673
ApoI RAATTY 1 cut(s) 455
Asp700I GAANNNNTTC 1 cut(s) 92
AspLEI GCGC 2 cut(s) 366, 368
AspS9I GGNCC 2 cut(s) 157, 790
AsuC2I CCSGG 1 cut(s) 717
AsuHPI GGTGA 1 cut(s) 298
AsuII TTCGAA 1 cut(s) 86
AvaI CYCGRG 1 cut(s) 51
BanII GRGCYC 2 cut(s) 124, 542
Bbv12I GWGCWC 1 cut(s) 542
BbvCI CCTCAGC 1 cut(s) 767
BbvI GCAGC 3 cut(s) 531, 657, 685
BccI CCATC 4 cut(s) 339, 418, 482, 751
BceAI ACGGC 3 cut(s) 248, 678, 838
BcgI CGANNNNNNTGC 2 cut(s) 619, 653
BciT130I CCWGG 1 cut(s) 356
BcnI CCSGG 1 cut(s) 717
BcoDI GTCTC 2 cut(s) 271, 475
BfmI CTRYAG 2 cut(s) 604, 671
BglII AGATCT 1 cut(s) 878
BisI GCNGC 7 cut(s) 443, 545, 671, 674, 687, 753, 826
BlsI GCNGC 7 cut(s) 444, 546, 672, 675, 688, 754, 827
Bme1390I CCNGG 2 cut(s) 356, 717
BmeT110I CYCGRG 1 cut(s) 51
BmgT120I GGNCC 2 cut(s) 157, 790
BmiI GGNNCC 1 cut(s) 791
BmrFI CCNGG 2 cut(s) 356, 717
BpmI CTGGAG 1 cut(s) 800
Bpu10I CCTNAGC 1 cut(s) 767
Bpu14I TTCGAA 1 cut(s) 86
BpuEI CTTGAG 1 cut(s) 239
BpuMI CCSGG 1 cut(s) 717
BsaHI GRCGYC 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 1 cut(s) 716
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 234, 799
Bse1I ACTGG 1 cut(s) 576
Bse3DI GCAATG 1 cut(s) 531
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 1 cut(s) 716
BseGI GGATG 1 cut(s) 11
BseLI CCNNNNNNNGG 3 cut(s) 118, 234, 799
BseMI GCAATG 1 cut(s) 531
BseMII CTCAG 2 cut(s) 758, 866
BseNI ACTGG 1 cut(s) 576
BsePI GCGCGC 1 cut(s) 364
BseRI GAGGAG 3 cut(s) 422, 605, 776
BseXI GCAGC 3 cut(s) 531, 657, 685
BseYI CCCAGC 2 cut(s) 150, 342
Bsh1236I CGCG 2 cut(s) 366, 368
BshFI GGCC 4 cut(s) 158, 297, 715, 792
BsiHKAI GWGCWC 1 cut(s) 542
BsiHKCI CYCGRG 1 cut(s) 51
BsiSI CCGG 3 cut(s) 294, 587, 716
BslFI GGGAC 2 cut(s) 206, 251
BslI CCNNNNNNNGG 3 cut(s) 118, 234, 799
BsmAI GTCTC 2 cut(s) 271, 475
BsmBI CGTCTC 1 cut(s) 271
BsmFI GGGAC 2 cut(s) 206, 251
BsnI GGCC 4 cut(s) 158, 297, 715, 792
Bso31I GGTCTC 1 cut(s) 475
BsoBI CYCGRG 1 cut(s) 51
Bsp119I TTCGAA 1 cut(s) 86
Bsp1286I GDGCHC 2 cut(s) 124, 542
BspANI GGCC 4 cut(s) 158, 297, 715, 792
BspCNI CTCAG 2 cut(s) 759, 867
BspFNI CGCG 2 cut(s) 366, 368
BspHI TCATGA 2 cut(s) 552, 651
BspLI GGNNCC 1 cut(s) 791
BspMAI CTGCAG 2 cut(s) 608, 675
BspPI GGATC 3 cut(s) 12, 65, 649
BspT104I TTCGAA 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 475
BsrDI GCAATG 1 cut(s) 531
BsrI ACTGG 1 cut(s) 576
BssECI CCNNGG 1 cut(s) 716
BssHII GCGCGC 1 cut(s) 364
BssNI GRCGYC 1 cut(s) 240
Bst2UI CCWGG 1 cut(s) 356
Bst6I CTCTTC 1 cut(s) 399
BstACI GRCGYC 1 cut(s) 240
BstBI TTCGAA 1 cut(s) 86
BstC8I GCNNGC 6 cut(s) 156, 244, 366, 542, 584, 901
BstDEI CTNAG 2 cut(s) 767, 875
BstF5I GGATG 1 cut(s) 11
BstFNI CGCG 2 cut(s) 366, 368
BstHHI GCGC 2 cut(s) 366, 368
BstMAI GTCTC 2 cut(s) 271, 475
BstMWI GCNNNNNNNGC 3 cut(s) 181, 269, 448
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 2 cut(s) 354, 715
BstSFI CTRYAG 2 cut(s) 604, 671
BstUI CGCG 2 cut(s) 366, 368
BstV1I GCAGC 3 cut(s) 531, 657, 685
BstX2I RGATCY 1 cut(s) 878
BstYI RGATCY 1 cut(s) 878
BsuRI GGCC 4 cut(s) 158, 297, 715, 792
BtgZI GCGATG 1 cut(s) 770
BtsCI GGATG 1 cut(s) 11
Cac8I GCNNGC 6 cut(s) 156, 244, 366, 542, 584, 901
CaiI CAGNNNCTG 1 cut(s) 445
CciI TCATGA 2 cut(s) 552, 651
CfoI GCGC 2 cut(s) 366, 368
Cfr13I GGNCC 2 cut(s) 157, 790
CseI GACGC 1 cut(s) 248
Csp6I GTAC 4 cut(s) 211, 520, 565, 616
CviAII CATG 6 cut(s) 97, 214, 248, 553, 631, 652
CviQI GTAC 4 cut(s) 211, 520, 565, 616
DdeI CTNAG 2 cut(s) 767, 875
DrdI GACNNNNNNGTC 1 cut(s) 467
DseDI GACNNNNNNGTC 1 cut(s) 467
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
EciI GGCGGA 2 cut(s) 250, 704
Ecl136II GAGCTC 1 cut(s) 540
Eco24I GRGCYC 2 cut(s) 124, 542
Eco31I GGTCTC 1 cut(s) 475
Eco32I GATATC 1 cut(s) 657
Eco53kI GAGCTC 1 cut(s) 540
Eco88I CYCGRG 1 cut(s) 51
EcoICRI GAGCTC 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 354
EcoRV GATATC 1 cut(s) 657
EcoT38I GRGCYC 2 cut(s) 124, 542
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 6 cut(s) 100, 217, 251, 556, 634, 655
FaiI YATR 8 cut(s) 98, 215, 249, 275, 554, 632, 653, 969
FaqI GGGAC 2 cut(s) 206, 251
FatI CATG 6 cut(s) 96, 213, 247, 552, 630, 651
FauI CCCGC 1 cut(s) 821
Fnu4HI GCNGC 7 cut(s) 443, 545, 671, 674, 687, 753, 826
FriOI GRGCYC 2 cut(s) 124, 542
Fsp4HI GCNGC 7 cut(s) 443, 545, 671, 674, 687, 753, 826
GlaI GCGC 2 cut(s) 365, 367
GluI GCNGC 7 cut(s) 443, 545, 671, 674, 687, 753, 826
GsaI CCCAGC 2 cut(s) 154, 346
GsuI CTGGAG 1 cut(s) 800
HaeIII GGCC 4 cut(s) 158, 297, 715, 792
HapII CCGG 3 cut(s) 294, 587, 716
HgaI GACGC 1 cut(s) 248
HhaI GCGC 2 cut(s) 366, 368
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 6 cut(s) 100, 217, 251, 556, 634, 655
Hin6I GCGC 2 cut(s) 364, 366
HinP1I GCGC 2 cut(s) 364, 366
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HindIII AAGCTT 2 cut(s) 230, 947
HinfI GANTC 6 cut(s) 49, 320, 435, 479, 513, 593
HpaII CCGG 3 cut(s) 294, 587, 716
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 2 cut(s) 820, 847
Hpy188I TCNGA 5 cut(s) 48, 398, 641, 707, 726
Hpy188III TCNNGA 5 cut(s) 68, 169, 553, 652, 739
Hpy8I GTNNAC 2 cut(s) 820, 847
Hpy99I CGWCG 5 cut(s) 65, 191, 209, 464, 664
HpyAV CCTTC 5 cut(s) 140, 170, 325, 458, 742
HpyCH4IV ACGT 1 cut(s) 534
HpyCH4V TGCA 4 cut(s) 606, 630, 673, 963
HpyF10VI GCNNNNNNNGC 3 cut(s) 181, 269, 448
HpyF3I CTNAG 2 cut(s) 767, 875
HpySE526I ACGT 1 cut(s) 534
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 6 cut(s) 100, 217, 251, 556, 634, 655
HspAI GCGC 2 cut(s) 364, 366
LmnI GCTCC 4 cut(s) 180, 230, 763, 775
Lsp1109I GCAGC 3 cut(s) 531, 657, 685
MaeII ACGT 1 cut(s) 534
MboII GAAGA 4 cut(s) 125, 416, 628, 955
MflI RGATCY 1 cut(s) 878
MhlI GDGCHC 2 cut(s) 124, 542
MluCI AATT 4 cut(s) 107, 280, 455, 927
MlyI GAGTC 4 cut(s) 43, 314, 522, 602
MroXI GAANNNNTTC 1 cut(s) 92
MseI TTAA 1 cut(s) 300
MspA1I CMGCKG 2 cut(s) 154, 445
MspI CCGG 3 cut(s) 294, 587, 716
MspR9I CCNGG 2 cut(s) 356, 717
MvaI CCWGG 1 cut(s) 356
MvnI CGCG 2 cut(s) 366, 368
MwoI GCNNNNNNNGC 3 cut(s) 181, 269, 448
NciI CCSGG 1 cut(s) 717
NlaIII CATG 6 cut(s) 100, 217, 251, 556, 634, 655
NlaIV GGNNCC 1 cut(s) 791
NspV TTCGAA 1 cut(s) 86
PaeR7I CTCGAG 1 cut(s) 51
PagI TCATGA 2 cut(s) 552, 651
PauI GCGCGC 1 cut(s) 364
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 752
PdmI GAANNNNTTC 1 cut(s) 92
PfeI GAWTC 2 cut(s) 435, 479
PflMI CCANNNNNTGG 1 cut(s) 234
PkrI GCNGC 7 cut(s) 444, 546, 672, 675, 688, 754, 827
PleI GAGTC 4 cut(s) 43, 314, 521, 601
PpsI GAGTC 4 cut(s) 43, 314, 521, 601
Psp124BI GAGCTC 1 cut(s) 542
Psp6I CCWGG 1 cut(s) 354
PspFI CCCAGC 2 cut(s) 150, 342
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 791
PspPI GGNCC 2 cut(s) 157, 790
PspXI VCTCGAGB 1 cut(s) 51
PstI CTGCAG 2 cut(s) 608, 675
PstNI CAGNNNCTG 1 cut(s) 445
PsuI RGATCY 1 cut(s) 878
PteI GCGCGC 1 cut(s) 364
PvuII CAGCTG 1 cut(s) 154
RsaI GTAC 4 cut(s) 212, 521, 566, 617
RsaNI GTAC 4 cut(s) 211, 520, 565, 616
SacI GAGCTC 1 cut(s) 542
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 7 cut(s) 443, 545, 671, 674, 687, 753, 826
Sau96I GGNCC 2 cut(s) 157, 790
SchI GAGTC 4 cut(s) 43, 314, 522, 602
ScrFI CCNGG 2 cut(s) 356, 717
SduI GDGCHC 2 cut(s) 124, 542
SfcI CTRYAG 2 cut(s) 604, 671
Sfr274I CTCGAG 1 cut(s) 51
SfuI TTCGAA 1 cut(s) 86
SlaI CTCGAG 1 cut(s) 51
SmlI CTYRAG 2 cut(s) 51, 254
SmoI CTYRAG 2 cut(s) 51, 254
Sse9I AATT 4 cut(s) 107, 280, 455, 927
SspI AATATT 1 cut(s) 895
SstI GAGCTC 1 cut(s) 542
StyD4I CCNGG 2 cut(s) 354, 715
TaiI ACGT 1 cut(s) 537
TasI AATT 4 cut(s) 107, 280, 455, 927
TatI WGTACW 1 cut(s) 210
TauI GCSGC 4 cut(s) 445, 689, 755, 828
TfiI GAWTC 2 cut(s) 435, 479
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TseI GCWGC 3 cut(s) 544, 670, 673
TspDTI ATGAA 3 cut(s) 85, 640, 939
TspGWI ACGGA 1 cut(s) 582
Van91I CCANNNNNTGG 1 cut(s) 234
XapI RAATTY 1 cut(s) 455
XhoI CTCGAG 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.