MD12G1145700.v1.1

expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010507
Physical Location & Seq
Reverse (-)
22481338 .. 22482877
1540 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1145700.v1.1.491

Sequence Viewer

Length: 981 bp
ATGGATGATCCTTCCTCCACCAACTCTGTCAATGGCTTCTTTTCCTTCCTGGCTCGTGGGGTGGACGATCTCGAAAGGGTCTATCTTTCCAGCAACTTCATGTCAATCCAATTCCTTCAAAGGGTTCTTACCCTTCTTCGATCTTTCCACTCCCAGCTGAACCTTCTTGTCCAAAAGCTCCATCTGCCTGTTGGGGACAAGTGGCTTGACGAGTACATGGACGAAAGCTCCAAGCTTTGGGAAGCCTGCCATGTCCTCAAATCCGCCGTCTCTGCCATGGAGAATTTCTACACCTCCGGCCACGCCATCACCTCCACCCTCCTCGACTCTCACCATCTCACTCCTCAGCTCTCCCGCCAGGTGGTGCGCGCGATTTCTCGGTGGCGGCGGGAAGCGTTTGGATTGGAGGAAGAGAACAGAGCGCTGATGGGAACGAGAATCCAACCACTCTCACTGAAATTCGACGAAAGGGTCGCCGTCGAATCGAAGCTTAATGGGTTCAGCGGGTTCAGGGGAGTCCTGTACGCAATGAGAAACGTGAGCTCTCTGCTTCTGATGATCTTGCTGTACGGACTGGTCTTCTGCTCGCCGGAGGACTCAACCTTTTGCAGGGAAGGAGACGACGACTACGAGGGCTGCATGATCTTCGGATCACCGTTCATGATATCGACGGCCAGACTGCAGCAGAGGGTGGCGGCGGAGATCGGGCAGATGAGCGAGAGGCCTGGGATTCTGGTGTGGGAGTTCAGGAGGTCGAAGGCAGCGATGGAGGAGCTGAGAGGTGAACTGGAGGAGAGGAGGGGCTCACAGGGAGGAGTGATGGATTGGGAGAGTGGGATTAGAGAGAGAGTGGAGCATCTGAGAGAGTGTTTTGGGGGGTTGACTTCTGGTTCTGAGAATATTGTGAGCCAACTTGATGATTTGTTTGATGAAATTGTTGAGGGGAGGAAGAAGCTGTTGGACTTTTGCAGTCACAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BPS1

Protein Analysis

327

Amino Acids

37.17

Weight (kDa)

5.4

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 470
AccB7I CCANNNNNTGG 1 cut(s) 237
AccII CGCG 2 cut(s) 369, 371
AciI CCGC 7 cut(s) 264, 355, 385, 388, 504, 695, 698
AclWI GGATC 2 cut(s) 2, 658
AcoI YGGCCR 2 cut(s) 298, 672
AcsI RAATTY 2 cut(s) 283, 458
AfaI GTAC 3 cut(s) 215, 524, 569
AfeI AGCGCT 1 cut(s) 423
AfiI CCNNNNNNNGG 4 cut(s) 121, 237, 361, 609
AgsI TTSAA 1 cut(s) 119
AjnI CCWGG 3 cut(s) 48, 357, 724
AjuI GAANNNNNNNTTGG 2 cut(s) 941, 973
AluBI AGCT 9 cut(s) 157, 178, 228, 235, 349, 490, 543, 775, 955
AluI AGCT 9 cut(s) 157, 178, 228, 235, 349, 490, 543, 775, 955
Alw21I GWGCWC 1 cut(s) 545
Alw26I GTCTC 2 cut(s) 274, 612
AlwI GGATC 2 cut(s) 2, 658
Aor51HI AGCGCT 1 cut(s) 423
AoxI GGCC 3 cut(s) 298, 672, 722
ApeKI GCWGC 3 cut(s) 636, 682, 761
ApoI RAATTY 2 cut(s) 283, 458
ArsI GACNNNNNNTTYG 2 cut(s) 252, 284
AspLEI GCGC 3 cut(s) 369, 371, 424
AsuHPI GGTGA 4 cut(s) 301, 323, 645, 794
BanII GRGCYC 2 cut(s) 545, 806
BauI CACGAG 1 cut(s) 54
BbsI GAAGAC 1 cut(s) 571
Bbv12I GWGCWC 1 cut(s) 545
BbvCI CCTCAGC 1 cut(s) 345
BbvI GCAGC 3 cut(s) 623, 694, 773
BccI CCATC 6 cut(s) 189, 314, 342, 421, 760, 814
BceAI ACGGC 3 cut(s) 251, 461, 687
BcgI CGANNNNNNTGC 2 cut(s) 628, 662
BciT130I CCWGG 3 cut(s) 50, 359, 726
BcoDI GTCTC 2 cut(s) 274, 612
BfmI CTRYAG 1 cut(s) 680
BfoI RGCGCY 1 cut(s) 425
BisI GCNGC 5 cut(s) 386, 637, 683, 696, 762
BlsI GCNGC 5 cut(s) 387, 638, 684, 697, 763
Bme1390I CCNGG 3 cut(s) 50, 359, 726
BmrFI CCNGG 3 cut(s) 50, 359, 726
BmsI GCATC 1 cut(s) 865
BpiI GAAGAC 1 cut(s) 571
BpmI CTGGAG 1 cut(s) 809
Bpu10I CCTNAGC 1 cut(s) 345
BsaJI CCNNGG 2 cut(s) 276, 725
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 4 cut(s) 121, 237, 361, 609
Bse1I ACTGG 2 cut(s) 579, 792
Bse3DI GCAATG 1 cut(s) 534
BseBI CCWGG 3 cut(s) 50, 359, 726
BseDI CCNNGG 2 cut(s) 276, 725
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 4 cut(s) 121, 237, 361, 609
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 4 cut(s) 359, 767, 851, 885
BseNI ACTGG 2 cut(s) 579, 792
BsePI GCGCGC 1 cut(s) 367
BseRI GAGGAG 6 cut(s) 311, 333, 785, 806, 811, 828
BseXI GCAGC 3 cut(s) 623, 694, 773
BseYI CCCAGC 1 cut(s) 153
Bsh1236I CGCG 2 cut(s) 369, 371
BshFI GGCC 3 cut(s) 300, 674, 724
BsiHKAI GWGCWC 1 cut(s) 545
BsiSI CCGG 2 cut(s) 297, 590
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 4 cut(s) 121, 237, 361, 609
BsmAI GTCTC 2 cut(s) 274, 612
BsmBI CGTCTC 2 cut(s) 274, 612
BsmFI GGGAC 1 cut(s) 209
BsnI GGCC 3 cut(s) 300, 674, 724
Bsp1286I GDGCHC 2 cut(s) 545, 806
Bsp143I GATC 7 cut(s) 7, 67, 140, 558, 642, 650, 702
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 7 cut(s) 264, 355, 385, 388, 504, 695, 698
BspANI GGCC 3 cut(s) 300, 674, 724
BspCNI CTCAG 4 cut(s) 358, 768, 852, 886
BspFNI CGCG 2 cut(s) 369, 371
BspHI TCATGA 1 cut(s) 660
BspMAI CTGCAG 1 cut(s) 684
BspPI GGATC 2 cut(s) 2, 658
BsrDI GCAATG 1 cut(s) 534
BsrI ACTGG 2 cut(s) 579, 792
BssECI CCNNGG 2 cut(s) 276, 725
BssHII GCGCGC 1 cut(s) 367
BssMI GATC 7 cut(s) 7, 67, 140, 558, 642, 650, 702
BssSI CACGAG 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 276
Bst2BI CACGAG 1 cut(s) 54
Bst2UI CCWGG 3 cut(s) 50, 359, 726
Bst4CI ACNGT 1 cut(s) 657
Bst6I CTCTTC 1 cut(s) 405
BstC8I GCNNGC 3 cut(s) 247, 369, 587
BstDEI CTNAG 4 cut(s) 345, 776, 860, 894
BstDSI CCRYGG 1 cut(s) 276
BstENI CCTNNNNNAGG 1 cut(s) 607
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 2 cut(s) 369, 371
BstH2I RGCGCY 1 cut(s) 425
BstHHI GCGC 3 cut(s) 369, 371, 424
BstKTI GATC 7 cut(s) 10, 70, 143, 561, 645, 653, 705
BstMAI GTCTC 2 cut(s) 274, 612
BstMBI GATC 7 cut(s) 7, 67, 140, 558, 642, 650, 702
BstMWI GCNNNNNNNGC 2 cut(s) 184, 272
BstNI CCWGG 3 cut(s) 50, 359, 726
BstSCI CCNGG 3 cut(s) 48, 357, 724
BstSFI CTRYAG 1 cut(s) 680
BstUI CGCG 2 cut(s) 369, 371
BstV1I GCAGC 3 cut(s) 623, 694, 773
BstV2I GAAGAC 1 cut(s) 571
BsuRI GGCC 3 cut(s) 300, 674, 724
BtgI CCRYGG 1 cut(s) 276
BtgZI GCGATG 1 cut(s) 779
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 1 cut(s) 452
Cac8I GCNNGC 3 cut(s) 247, 369, 587
CciI TCATGA 1 cut(s) 660
CfoI GCGC 3 cut(s) 369, 371, 424
Csp6I GTAC 3 cut(s) 214, 523, 568
CviAII CATG 6 cut(s) 100, 217, 251, 277, 640, 661
CviQI GTAC 3 cut(s) 214, 523, 568
DdeI CTNAG 4 cut(s) 345, 776, 860, 894
DpnI GATC 7 cut(s) 9, 69, 142, 560, 644, 652, 704
DpnII GATC 7 cut(s) 7, 67, 140, 558, 642, 650, 702
DrdI GACNNNNNNGTC 1 cut(s) 470
DseDI GACNNNNNNGTC 1 cut(s) 470
EaeI YGGCCR 2 cut(s) 298, 672
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
EciI GGCGGA 2 cut(s) 253, 713
Ecl136II GAGCTC 1 cut(s) 543
Eco130I CCWWGG 1 cut(s) 276
Eco147I AGGCCT 1 cut(s) 724
Eco24I GRGCYC 2 cut(s) 545, 806
Eco32I GATATC 1 cut(s) 666
Eco47III AGCGCT 1 cut(s) 423
Eco53kI GAGCTC 1 cut(s) 543
EcoICRI GAGCTC 1 cut(s) 543
EcoNI CCTNNNNNAGG 1 cut(s) 607
EcoRII CCWGG 3 cut(s) 48, 357, 724
EcoRV GATATC 1 cut(s) 666
EcoT14I CCWWGG 1 cut(s) 276
EcoT38I GRGCYC 2 cut(s) 545, 806
ErhI CCWWGG 1 cut(s) 276
Esp3I CGTCTC 2 cut(s) 274, 612
FaeI CATG 6 cut(s) 103, 220, 254, 280, 643, 664
FaiI YATR 6 cut(s) 101, 218, 252, 278, 641, 662
FaqI GGGAC 1 cut(s) 209
FatI CATG 6 cut(s) 99, 216, 250, 276, 639, 660
FauI CCCGC 3 cut(s) 362, 381, 497
Fnu4HI GCNGC 5 cut(s) 386, 637, 683, 696, 762
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 2 cut(s) 545, 806
Fsp4HI GCNGC 5 cut(s) 386, 637, 683, 696, 762
GlaI GCGC 3 cut(s) 368, 370, 423
GluI GCNGC 5 cut(s) 386, 637, 683, 696, 762
GsaI CCCAGC 1 cut(s) 157
GsuI CTGGAG 1 cut(s) 809
HaeII RGCGCY 1 cut(s) 425
HaeIII GGCC 3 cut(s) 300, 674, 724
HapII CCGG 2 cut(s) 297, 590
HhaI GCGC 3 cut(s) 369, 371, 424
Hin1II CATG 6 cut(s) 103, 220, 254, 280, 643, 664
Hin6I GCGC 3 cut(s) 367, 369, 422
HinP1I GCGC 3 cut(s) 367, 369, 422
HincII GTYRAC 1 cut(s) 882
HindII GTYRAC 1 cut(s) 882
HindIII AAGCTT 2 cut(s) 233, 488
HinfI GANTC 6 cut(s) 326, 438, 482, 516, 596, 730
HpaII CCGG 2 cut(s) 297, 590
HphI GGTGA 4 cut(s) 301, 323, 645, 794
Hpy166II GTNNAC 3 cut(s) 64, 785, 882
Hpy188I TCNGA 4 cut(s) 555, 650, 861, 895
Hpy188III TCNNGA 3 cut(s) 71, 661, 748
Hpy8I GTNNAC 3 cut(s) 64, 785, 882
Hpy99I CGWCG 4 cut(s) 467, 482, 626, 673
HpyAV CCTTC 7 cut(s) 21, 55, 125, 143, 173, 608, 751
HpyCH4III ACNGT 1 cut(s) 657
HpyCH4IV ACGT 1 cut(s) 537
HpyCH4V TGCA 4 cut(s) 609, 639, 682, 969
HpyF10VI GCNNNNNNNGC 2 cut(s) 184, 272
HpyF3I CTNAG 4 cut(s) 345, 776, 860, 894
HpySE526I ACGT 1 cut(s) 537
Hsp92II CATG 6 cut(s) 103, 220, 254, 280, 643, 664
HspAI GCGC 3 cut(s) 367, 369, 422
Kzo9I GATC 7 cut(s) 7, 67, 140, 558, 642, 650, 702
LmnI GCTCC 4 cut(s) 183, 233, 772, 853
Lsp1109I GCAGC 3 cut(s) 623, 694, 773
LweI GCATC 1 cut(s) 865
MaeII ACGT 1 cut(s) 537
MaeIII GTNAC 1 cut(s) 971
MalI GATC 7 cut(s) 9, 69, 142, 560, 644, 652, 704
MboI GATC 7 cut(s) 7, 67, 140, 558, 642, 650, 702
MboII GAAGA 5 cut(s) 128, 422, 571, 637, 961
MhlI GDGCHC 2 cut(s) 545, 806
MluCI AATT 4 cut(s) 110, 283, 458, 933
MlyI GAGTC 3 cut(s) 320, 525, 590
MmeI TCCRAC 2 cut(s) 466, 939
MseI TTAA 1 cut(s) 492
MspA1I CMGCKG 2 cut(s) 157, 504
MspI CCGG 2 cut(s) 297, 590
MspR9I CCNGG 3 cut(s) 50, 359, 726
MvaI CCWGG 3 cut(s) 50, 359, 726
MvnI CGCG 2 cut(s) 369, 371
MwoI GCNNNNNNNGC 2 cut(s) 184, 272
NcoI CCATGG 1 cut(s) 276
NdeII GATC 7 cut(s) 7, 67, 140, 558, 642, 650, 702
NlaIII CATG 6 cut(s) 103, 220, 254, 280, 643, 664
NmuCI GTSAC 1 cut(s) 971
PagI TCATGA 1 cut(s) 660
PauI GCGCGC 1 cut(s) 367
PceI AGGCCT 1 cut(s) 724
PcsI WCGNNNNNNNCGW 2 cut(s) 627, 761
PfeI GAWTC 3 cut(s) 438, 482, 730
PflMI CCANNNNNTGG 1 cut(s) 237
PkrI GCNGC 5 cut(s) 387, 638, 684, 697, 763
PleI GAGTC 3 cut(s) 320, 524, 590
PpsI GAGTC 3 cut(s) 320, 524, 590
Psp124BI GAGCTC 1 cut(s) 545
Psp6I CCWGG 3 cut(s) 48, 357, 724
PspFI CCCAGC 1 cut(s) 153
PspGI CCWGG 3 cut(s) 48, 357, 724
PstI CTGCAG 1 cut(s) 684
PteI GCGCGC 1 cut(s) 367
PvuII CAGCTG 1 cut(s) 157
RsaI GTAC 3 cut(s) 215, 524, 569
RsaNI GTAC 3 cut(s) 214, 523, 568
SacI GAGCTC 1 cut(s) 545
SaqAI TTAA 1 cut(s) 492
SatI GCNGC 5 cut(s) 386, 637, 683, 696, 762
Sau3AI GATC 7 cut(s) 7, 67, 140, 558, 642, 650, 702
SchI GAGTC 3 cut(s) 320, 525, 590
ScrFI CCNGG 3 cut(s) 50, 359, 726
SduI GDGCHC 2 cut(s) 545, 806
SfaNI GCATC 1 cut(s) 865
SfcI CTRYAG 1 cut(s) 680
Sse9I AATT 4 cut(s) 110, 283, 458, 933
SseBI AGGCCT 1 cut(s) 724
SsiI CCGC 7 cut(s) 264, 355, 385, 388, 504, 695, 698
SspI AATATT 1 cut(s) 901
SstI GAGCTC 1 cut(s) 545
StuI AGGCCT 1 cut(s) 724
StyD4I CCNGG 3 cut(s) 48, 357, 724
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 1 cut(s) 657
TaiI ACGT 1 cut(s) 540
TaqI TCGA 8 cut(s) 72, 139, 324, 462, 480, 485, 668, 755
TasI AATT 4 cut(s) 110, 283, 458, 933
TatI WGTACW 1 cut(s) 213
TauI GCSGC 2 cut(s) 388, 698
TfiI GAWTC 3 cut(s) 438, 482, 730
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 1 cut(s) 459
TseFI GTSAC 1 cut(s) 971
TseI GCWGC 3 cut(s) 636, 682, 761
Tsp45I GTSAC 1 cut(s) 971
TspDTI ATGAA 3 cut(s) 88, 649, 945
TspGWI ACGGA 1 cut(s) 585
TspRI CASTG 1 cut(s) 459
Van91I CCANNNNNTGG 1 cut(s) 237
XagI CCTNNNNNAGG 1 cut(s) 607
XapI RAATTY 2 cut(s) 283, 458
XcmI CCANNNNNNNNNTGG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.