MD04G1137400.v1.1

Protein RETICULATA-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009890
Physical Location & Seq
Forward (+)
22502005 .. 22505437
3433 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1137400.v1.1.491

Sequence Viewer

Length: 1341 bp
ATGGCGGCTTGTTCTTCGAGCTTTGGGATATCGAATGTTGCAACTTTGCGGAACGAGGTCGTTCGGAAGAAGATGTGGAGCCAGAATTTGATTTCTTATGGAATAAGGTTTAGGAATGATGCAAAGGAGGTTGCTTTTCCAGTGCTCTATAGGAATAGTAGCTTTAGAGATAGGCTGAATTCGTTTTCGGTGAAGAAAGACCGGAAATTTCCGGTCATGTCAATGTCCGAGGCTCGGGCTGAATTACAATCCGAGTTAGGCAAAATAGCTGTTTCGAATGAGGGAGGAGATGTTATTGTAGAAAAGGAAATGAGGATTTCGGAGAATGAGTGTGGATCAAAAACCGAGAGTGGTGGTAGTGATAAGGATATGATTGATGGTAGTGGTGATAATGGTAAATATACGAACGGTAGAGGTGGAGGAGGAGGTGGTGGTGGTGGTGACAGTGATGGGGGTGATAAAGAAGAAGAAGAGTTTGGGCCAATTATGAAATTTGAAGAGATTATGAAAGAGACTGAGGCTCGAGGGGCTAGTCTTCCCTCCGATATGATTGAGGCTGCCAAGAGCGTGGGAATTCGCAAAGTGCTTCTCCTTAGATATCTGGATTTGCAGGGGTCAGCCTGGCCTCTAGGTTTCTTAATGAGGTCATGCTCTATGCTTCGCGATCGAATGCTTGCTGATCCATCATTTCTCTTCAAAATTGGAACAGAGATAGTCATTGATTCTTGTTGTGCTACATTTGCGGAAGTTCAAAAGAGGGGCAAAGACTTCTGGGCAGAATTTGAGCTGTATGTTGCAGATCTTTTGGTTGGGGTGGTGGTTAATGTTGCTTTGGTTGGGATGTTGGCCCCCTATGCTCGTATTGGGAAACCATCATTATCTAAAGGGTTGCTTGGACGCTTGCAACATTCTTATGGAGCTCTTCCTAGCAGTGTATTTGAAGCGGAAAGGCCAGGATGTAGATATTCTGTAAAGCAGAGAATTGCCACTTACTTTTTTAAGGGCATTTTGTATGGAGCGGTAGGCTTTGGATGTGGTATTATAGGCCAAGGAATTGCGAACTTGATCATGACTGCCAAGCGGAGCATAAAGAAGTCAGAAGAGGACATACCTGTTCCACCTCTAATTAAGAGTGCTGCTCTTTGGGGTGTTTTCCTTGCTGTCTCGTCCAACACCCGCTATCAAATCATCAATGGACTTGAACGCTTGGTAGAGGCATCGCCTTTGGCGAAACAGAACCCACTTGTTGCAATGGCTTTTACTGTCGGAGTACGATTTTCAAACAATGTTTATGGGGGGATGCAGTTCGTAGACTGGGCTAGATGGAGTGGGGTGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RETICULATA-like

Protein Analysis

447

Amino Acids

48.63

Weight (kDa)

8.79

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RETICULATA-like PF11891 222 - 400 1.9e-65 Protein RETICULATA-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1019
AccI GTMKAC 1 cut(s) 1311
AccII CGCG 1 cut(s) 663
AciI CCGC 7 cut(s) 5, 49, 743, 944, 1019, 1081, 1177
AclWI GGATC 2 cut(s) 343, 674
AcsI RAATTY 6 cut(s) 85, 178, 206, 491, 573, 779
AfaI GTAC 1 cut(s) 1272
AfiI CCNNNNNNNGG 1 cut(s) 234
AgsI TTSAA 6 cut(s) 497, 697, 752, 941, 1202, 1281
AjnI CCWGG 2 cut(s) 620, 952
AjuI GAANNNNNNNTTGG 2 cut(s) 459, 491
AluBI AGCT 5 cut(s) 21, 162, 269, 787, 920
AluI AGCT 5 cut(s) 21, 162, 269, 787, 920
Alw21I GWGCWC 2 cut(s) 147, 922
Alw26I GTCTC 2 cut(s) 506, 1168
AlwI GGATC 2 cut(s) 343, 674
Ama87I CYCGRG 2 cut(s) 234, 522
AoxI GGCC 5 cut(s) 479, 623, 846, 950, 1045
ApeKI GCWGC 2 cut(s) 557, 1136
ApoI RAATTY 6 cut(s) 85, 178, 206, 491, 573, 779
AspS9I GGNCC 2 cut(s) 479, 847
AsuHPI GGTGA 4 cut(s) 202, 398, 452, 467
AsuII TTCGAA 1 cut(s) 275
AvaI CYCGRG 2 cut(s) 234, 522
BanII GRGCYC 1 cut(s) 922
BarI GAAGNNNNNNTAC 2 cut(s) 1092, 1124
BbsI GAAGAC 1 cut(s) 527
Bbv12I GWGCWC 2 cut(s) 147, 922
BbvI GCAGC 2 cut(s) 544, 1123
BccI CCATC 5 cut(s) 371, 443, 691, 880, 1317
BciT130I CCWGG 2 cut(s) 622, 954
BclI TGATCA 1 cut(s) 1065
BcoDI GTCTC 2 cut(s) 506, 1168
BfaI CTAG 4 cut(s) 531, 629, 927, 1320
BfmI CTRYAG 1 cut(s) 148
BglII AGATCT 1 cut(s) 799
BisI GCNGC 3 cut(s) 6, 558, 1137
BlsI GCNGC 3 cut(s) 7, 559, 1138
Bme1390I CCNGG 2 cut(s) 622, 954
BmeT110I CYCGRG 2 cut(s) 234, 522
BmgT120I GGNCC 2 cut(s) 479, 847
BmiI GGNNCC 2 cut(s) 80, 849
BmrFI CCNGG 2 cut(s) 622, 954
BmrI ACTGGG 1 cut(s) 1324
BmsI GCATC 3 cut(s) 109, 1226, 1290
BmuI ACTGGG 1 cut(s) 1324
BpiI GAAGAC 1 cut(s) 527
BplI GAGNNNNNCTC 2 cut(s) 1123, 1155
Bpu14I TTCGAA 1 cut(s) 275
BsaJI CCNNGG 2 cut(s) 228, 1048
BsaWI WCCGGW 2 cut(s) 201, 211
BsaXI ACNNNNNCTCC 4 cut(s) 276, 306, 314, 344
Bsc4I CCNNNNNNNGG 1 cut(s) 234
Bse1I ACTGG 2 cut(s) 140, 1319
Bse3DI GCAATG 1 cut(s) 1257
BseBI CCWGG 2 cut(s) 622, 954
BseDI CCNNGG 2 cut(s) 228, 1048
BseGI GGATG 4 cut(s) 846, 962, 1037, 1305
BseLI CCNNNNNNNGG 1 cut(s) 234
BseMI GCAATG 1 cut(s) 1257
BseMII CTCAG 1 cut(s) 507
BseNI ACTGG 2 cut(s) 140, 1319
BseRI GAGGAG 3 cut(s) 300, 435, 438
BseXI GCAGC 2 cut(s) 544, 1123
Bsh1236I CGCG 1 cut(s) 663
Bsh1285I CGRYCG 1 cut(s) 667
BshFI GGCC 5 cut(s) 481, 625, 848, 952, 1047
BsiEI CGRYCG 1 cut(s) 667
BsiHKAI GWGCWC 2 cut(s) 147, 922
BsiHKCI CYCGRG 2 cut(s) 234, 522
BsiSI CCGG 2 cut(s) 202, 212
BslI CCNNNNNNNGG 1 cut(s) 234
BsmAI GTCTC 2 cut(s) 506, 1168
BsmI GAATGC 1 cut(s) 675
BsnI GGCC 5 cut(s) 481, 625, 848, 952, 1047
BsoBI CYCGRG 2 cut(s) 234, 522
Bsp119I TTCGAA 1 cut(s) 275
Bsp1286I GDGCHC 2 cut(s) 147, 922
Bsp143I GATC 5 cut(s) 335, 664, 679, 799, 1065
Bsp68I TCGCGA 1 cut(s) 663
BspACI CCGC 7 cut(s) 5, 49, 743, 944, 1019, 1081, 1177
BspANI GGCC 5 cut(s) 481, 625, 848, 952, 1047
BspCNI CTCAG 1 cut(s) 508
BspFNI CGCG 1 cut(s) 663
BspHI TCATGA 1 cut(s) 1068
BspLI GGNNCC 2 cut(s) 80, 849
BspPI GGATC 2 cut(s) 343, 674
BspQI GCTCTTC 1 cut(s) 927
BspT104I TTCGAA 1 cut(s) 275
BsrBI CCGCTC 1 cut(s) 1019
BsrDI GCAATG 1 cut(s) 1257
BsrI ACTGG 2 cut(s) 140, 1319
BssECI CCNNGG 2 cut(s) 228, 1048
BssMI GATC 5 cut(s) 335, 664, 679, 799, 1065
BssT1I CCWWGG 1 cut(s) 1048
Bst2UI CCWGG 2 cut(s) 622, 954
Bst4CI ACNGT 3 cut(s) 410, 446, 1264
Bst6I CTCTTC 5 cut(s) 465, 492, 698, 927, 1095
BstBI TTCGAA 1 cut(s) 275
BstC8I GCNNGC 2 cut(s) 675, 902
BstDEI CTNAG 2 cut(s) 516, 593
BstF5I GGATG 4 cut(s) 846, 962, 1037, 1305
BstFNI CGCG 1 cut(s) 663
BstKTI GATC 5 cut(s) 338, 667, 682, 802, 1068
BstMAI GTCTC 2 cut(s) 506, 1168
BstMBI GATC 5 cut(s) 335, 664, 679, 799, 1065
BstMCI CGRYCG 1 cut(s) 667
BstMWI GCNNNNNNNGC 3 cut(s) 527, 740, 854
BstNI CCWGG 2 cut(s) 622, 954
BstSCI CCNGG 2 cut(s) 620, 952
BstSFI CTRYAG 1 cut(s) 148
BstUI CGCG 1 cut(s) 663
BstV1I GCAGC 2 cut(s) 544, 1123
BstV2I GAAGAC 1 cut(s) 527
BstX2I RGATCY 1 cut(s) 799
BstXI CCANNNNNNTGG 1 cut(s) 568
BstYI RGATCY 1 cut(s) 799
BsuRI GGCC 5 cut(s) 481, 625, 848, 952, 1047
BtgZI GCGATG 1 cut(s) 1203
BtsCI GGATG 4 cut(s) 846, 962, 1037, 1305
BtsI GCAGTG 1 cut(s) 937
BtsIMutI CAGTG 3 cut(s) 147, 451, 937
BtuMI TCGCGA 1 cut(s) 663
Cac8I GCNNGC 2 cut(s) 675, 902
CciI TCATGA 1 cut(s) 1068
Cfr13I GGNCC 2 cut(s) 479, 847
CseI GACGC 1 cut(s) 906
Csp6I GTAC 1 cut(s) 1271
CviAII CATG 3 cut(s) 217, 648, 1069
CviQI GTAC 1 cut(s) 1271
DdeI CTNAG 2 cut(s) 516, 593
DpnI GATC 5 cut(s) 337, 666, 681, 801, 1067
DpnII GATC 5 cut(s) 335, 664, 679, 799, 1065
Eam1104I CTCTTC 5 cut(s) 465, 492, 698, 927, 1095
EarI CTCTTC 5 cut(s) 465, 492, 698, 927, 1095
Ecl136II GAGCTC 1 cut(s) 920
Eco130I CCWWGG 1 cut(s) 1048
Eco24I GRGCYC 1 cut(s) 922
Eco32I GATATC 2 cut(s) 30, 599
Eco53kI GAGCTC 1 cut(s) 920
Eco88I CYCGRG 2 cut(s) 234, 522
EcoICRI GAGCTC 1 cut(s) 920
EcoRI GAATTC 2 cut(s) 178, 573
EcoRII CCWGG 2 cut(s) 620, 952
EcoRV GATATC 2 cut(s) 30, 599
EcoT14I CCWWGG 1 cut(s) 1048
EcoT38I GRGCYC 1 cut(s) 922
ErhI CCWWGG 1 cut(s) 1048
FaeI CATG 3 cut(s) 220, 651, 1072
FalI AAGNNNNNCTT 2 cut(s) 876, 908
FatI CATG 3 cut(s) 216, 647, 1068
FauI CCCGC 1 cut(s) 1184
FbaI TGATCA 1 cut(s) 1065
FblI GTMKAC 1 cut(s) 1311
Fnu4HI GCNGC 3 cut(s) 6, 558, 1137
FokI GGATG 4 cut(s) 853, 969, 1044, 1312
FriOI GRGCYC 1 cut(s) 922
Fsp4HI GCNGC 3 cut(s) 6, 558, 1137
FspBI CTAG 4 cut(s) 531, 629, 927, 1320
GluI GCNGC 3 cut(s) 6, 558, 1137
HaeIII GGCC 5 cut(s) 481, 625, 848, 952, 1047
HapII CCGG 2 cut(s) 202, 212
HgaI GACGC 1 cut(s) 906
Hin1II CATG 3 cut(s) 220, 651, 1072
HinfI GANTC 1 cut(s) 722
HpaII CCGG 2 cut(s) 202, 212
HphI GGTGA 4 cut(s) 202, 398, 452, 467
Hpy166II GTNNAC 1 cut(s) 1312
Hpy188I TCNGA 7 cut(s) 66, 229, 253, 322, 544, 1099, 1268
Hpy188III TCNNGA 3 cut(s) 602, 662, 1069
Hpy8I GTNNAC 1 cut(s) 1312
HpyCH4III ACNGT 3 cut(s) 410, 446, 1264
HpyCH4V TGCA 8 cut(s) 41, 122, 610, 797, 904, 1250, 1303, 1336
HpyF10VI GCNNNNNNNGC 3 cut(s) 527, 740, 854
HpyF3I CTNAG 2 cut(s) 516, 593
Hsp92II CATG 3 cut(s) 220, 651, 1072
Ksp22I TGATCA 1 cut(s) 1065
Kzo9I GATC 5 cut(s) 335, 664, 679, 799, 1065
LguI GCTCTTC 1 cut(s) 927
LmnI GCTCC 4 cut(s) 78, 917, 1016, 1083
Lsp1109I GCAGC 2 cut(s) 544, 1123
LweI GCATC 3 cut(s) 109, 1226, 1290
MaeI CTAG 4 cut(s) 531, 629, 927, 1320
MaeIII GTNAC 1 cut(s) 440
MalI GATC 5 cut(s) 337, 666, 681, 801, 1067
MbiI CCGCTC 1 cut(s) 1019
MboI GATC 5 cut(s) 335, 664, 679, 799, 1065
MflI RGATCY 1 cut(s) 799
MhlI GDGCHC 2 cut(s) 147, 922
MmeI TCCRAC 2 cut(s) 1194, 1246
MseI TTAA 4 cut(s) 638, 822, 999, 1128
MslI CAYNNNNRTG 2 cut(s) 221, 912
MspI CCGG 2 cut(s) 202, 212
MspR9I CCNGG 2 cut(s) 622, 954
Mva1269I GAATGC 1 cut(s) 675
MvaI CCWGG 2 cut(s) 622, 954
MvnI CGCG 1 cut(s) 663
MwoI GCNNNNNNNGC 3 cut(s) 527, 740, 854
NdeII GATC 5 cut(s) 335, 664, 679, 799, 1065
NlaIII CATG 3 cut(s) 220, 651, 1072
NlaIV GGNNCC 2 cut(s) 80, 849
NmuCI GTSAC 1 cut(s) 440
NruI TCGCGA 1 cut(s) 663
NspV TTCGAA 1 cut(s) 275
PaeR7I CTCGAG 1 cut(s) 522
PagI TCATGA 1 cut(s) 1068
PciSI GCTCTTC 1 cut(s) 927
PcsI WCGNNNNNNNCGW 1 cut(s) 1226
PctI GAATGC 1 cut(s) 675
PfeI GAWTC 1 cut(s) 722
PkrI GCNGC 3 cut(s) 7, 559, 1138
Ple19I CGATCG 1 cut(s) 667
Psp124BI GAGCTC 1 cut(s) 922
Psp6I CCWGG 2 cut(s) 620, 952
PspGI CCWGG 2 cut(s) 620, 952
PspN4I GGNNCC 2 cut(s) 80, 849
PspPI GGNCC 2 cut(s) 479, 847
PspXI VCTCGAGB 1 cut(s) 522
PsuI RGATCY 1 cut(s) 799
PvuI CGATCG 1 cut(s) 667
RruI TCGCGA 1 cut(s) 663
RsaI GTAC 1 cut(s) 1272
RsaNI GTAC 1 cut(s) 1271
RseI CAYNNNNRTG 2 cut(s) 221, 912
SacI GAGCTC 1 cut(s) 922
SapI GCTCTTC 1 cut(s) 927
SaqAI TTAA 4 cut(s) 638, 822, 999, 1128
SatI GCNGC 3 cut(s) 6, 558, 1137
Sau3AI GATC 5 cut(s) 335, 664, 679, 799, 1065
Sau96I GGNCC 2 cut(s) 479, 847
ScrFI CCNGG 2 cut(s) 622, 954
SduI GDGCHC 2 cut(s) 147, 922
SfaNI GCATC 3 cut(s) 109, 1226, 1290
SfcI CTRYAG 1 cut(s) 148
Sfr274I CTCGAG 1 cut(s) 522
SfuI TTCGAA 1 cut(s) 275
SlaI CTCGAG 1 cut(s) 522
SmiMI CAYNNNNRTG 2 cut(s) 221, 912
SmlI CTYRAG 1 cut(s) 522
SmoI CTYRAG 1 cut(s) 522
SsiI CCGC 7 cut(s) 5, 49, 743, 944, 1019, 1081, 1177
SspMI CTAG 4 cut(s) 531, 629, 927, 1320
SstI GAGCTC 1 cut(s) 922
StyD4I CCNGG 2 cut(s) 620, 952
StyI CCWWGG 1 cut(s) 1048
TaaI ACNGT 3 cut(s) 410, 446, 1264
TaqI TCGA 5 cut(s) 17, 32, 275, 523, 667
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 722
Tru1I TTAA 4 cut(s) 638, 822, 999, 1128
Tru9I TTAA 4 cut(s) 638, 822, 999, 1128
TscAI CASTG 3 cut(s) 147, 451, 937
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 2 cut(s) 557, 1136
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 2 cut(s) 503, 521
TspRI CASTG 3 cut(s) 147, 451, 937
XapI RAATTY 6 cut(s) 85, 178, 206, 491, 573, 779
XhoI CTCGAG 1 cut(s) 522
XmiI GTMKAC 1 cut(s) 1311
XspI CTAG 4 cut(s) 531, 629, 927, 1320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.