Rorug03G0074500

Protein RETICULATA-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0009890
Physical Location & Seq
Reverse (-)
5857733 .. 5860209
2477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0074500.1

Sequence Viewer

Length: 582 bp
ATGTTCTTGTTCGATTGGTTCTATGGGGTTCTCGCGTCGCTAGGCTTGTGGCAGAAGGAGGCCAAGATCTTGTTCTTGGGTCTCGATAATGCCGGCAAAACCACCTTGCTTCATATGCTTAAAGACGAGAGATTAGTTCAGCACCAACCGACTCAGTATCCGACATCTGAGGAGTTAAGCATTGGGAAAATCAAGTTCAAGGCTTTTGATTTGGGCGGGCATCAGATTGCTCGCAGAGTTTGGAAAGACTACTATGCCAAGGTGGATGCAGTTGTTTACCTAGTGGATTCTTATGACAAGGAAAGATTCGCGGAATCAAAAAAGGAGCTGGATGCTCTCCTCTCTGATGAGGCATTGGCCACTGTACCATTCCTTATTCTGGGAAACAAGATTGACATACCATATGCGGCATCAGAAGAAGAGTTGCGATATCACCTTGGTCTGAACAACTTCACCACAGGCAAGGGTAAAGTGAACTTGGTAGACTCGAACGTGCGCCCTCTCGAAGTATTCATGTGCAGCATTGTTCGCAAAATGGGTTATGGAGATGGATTCAAGTGGGTTTCTCAGTACATCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RETICULATA-like

Protein Analysis

193

Amino Acids

22.11

Weight (kDa)

6.91

Isoelectric Point (pI)

28.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 192 3.4e-64 ADP-ribosylation factor family
SRPRB PF09439 19 - 145 5.4e-09 Signal recognition particle receptor beta subunit
Roc PF08477 22 - 132 2.2e-08 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 22 - 130 4.1e-07 50S ribosome-binding GTPase
Ras PF00071 22 - 134 1.8e-06 Ras family
G-alpha PF00503 51 - 133 8.8e-09 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 483
AccII CGCG 2 cut(s) 35, 311
AciI CCGC 3 cut(s) 216, 311, 407
AcoI YGGCCR 1 cut(s) 357
AfaI GTAC 2 cut(s) 366, 572
AfiI CCNNNNNNNGG 1 cut(s) 379
AgsI TTSAA 2 cut(s) 199, 556
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 1 cut(s) 328
AluI AGCT 1 cut(s) 328
Alw26I GTCTC 1 cut(s) 86
AoxI GGCC 2 cut(s) 60, 357
ApeKI GCWGC 1 cut(s) 519
Asp700I GAANNNNTTC 1 cut(s) 449
AspLEI GCGC 1 cut(s) 498
AsuHPI GGTGA 2 cut(s) 425, 445
BalI TGGCCA 1 cut(s) 359
BbvI GCAGC 1 cut(s) 531
BccI CCATC 1 cut(s) 542
BciVI GTATCC 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 2 cut(s) 41, 281
BfuI GTATCC 1 cut(s) 168
BglII AGATCT 1 cut(s) 66
BisI GCNGC 2 cut(s) 408, 520
BlsI GCNGC 2 cut(s) 409, 521
BmsI GCATC 4 cut(s) 229, 256, 322, 419
BsaI GGTCTC 1 cut(s) 86
BsaJI CCNNGG 2 cut(s) 258, 436
Bsc4I CCNNNNNNNGG 1 cut(s) 379
Bse118I RCCGGY 1 cut(s) 92
BseDI CCNNGG 2 cut(s) 258, 436
BseGI GGATG 2 cut(s) 271, 337
BseLI CCNNNNNNNGG 1 cut(s) 379
BseMII CTCAG 3 cut(s) 159, 167, 581
BseRI GAGGAG 2 cut(s) 185, 329
BseXI GCAGC 1 cut(s) 531
BsgI GTGCAG 1 cut(s) 538
Bsh1236I CGCG 2 cut(s) 35, 311
BshFI GGCC 2 cut(s) 62, 359
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 1 cut(s) 379
BsmAI GTCTC 1 cut(s) 86
BsnI GGCC 2 cut(s) 62, 359
Bso31I GGTCTC 1 cut(s) 86
Bsp143I GATC 1 cut(s) 66
BspACI CCGC 3 cut(s) 216, 311, 407
BspANI GGCC 2 cut(s) 62, 359
BspCNI CTCAG 3 cut(s) 160, 166, 580
BspFNI CGCG 2 cut(s) 35, 311
BspTNI GGTCTC 1 cut(s) 86
BsrFI RCCGGY 1 cut(s) 92
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 2 cut(s) 258, 436
BssMI GATC 1 cut(s) 66
BssT1I CCWWGG 2 cut(s) 258, 436
Bst4CI ACNGT 1 cut(s) 364
Bst6I CTCTTC 1 cut(s) 414
BstC8I GCNNGC 3 cut(s) 94, 218, 232
BstDEI CTNAG 3 cut(s) 153, 168, 567
BstF5I GGATG 2 cut(s) 271, 337
BstFNI CGCG 2 cut(s) 35, 311
BstHHI GCGC 1 cut(s) 498
BstKTI GATC 1 cut(s) 69
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 2 cut(s) 115, 528
BstUI CGCG 2 cut(s) 35, 311
BstV1I GCAGC 1 cut(s) 531
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuI GTATCC 1 cut(s) 168
BsuRI GGCC 2 cut(s) 62, 359
BtsCI GGATG 2 cut(s) 271, 337
BtsIMutI CAGTG 1 cut(s) 360
Cac8I GCNNGC 3 cut(s) 94, 218, 232
CfoI GCGC 1 cut(s) 498
Cfr10I RCCGGY 1 cut(s) 92
CseI GACGC 1 cut(s) 24
Csp6I GTAC 2 cut(s) 365, 571
CviAII CATG 1 cut(s) 514
CviJI RGCY 5 cut(s) 45, 62, 203, 328, 359
CviKI_1 RGCY 5 cut(s) 45, 62, 203, 328, 359
CviQI GTAC 2 cut(s) 365, 571
DdeI CTNAG 3 cut(s) 153, 168, 567
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
EaeI YGGCCR 1 cut(s) 357
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
Eco130I CCWWGG 2 cut(s) 258, 436
Eco31I GGTCTC 1 cut(s) 86
Eco32I GATATC 1 cut(s) 431
EcoRV GATATC 1 cut(s) 431
EcoT14I CCWWGG 2 cut(s) 258, 436
ErhI CCWWGG 2 cut(s) 258, 436
FaeI CATG 1 cut(s) 517
FatI CATG 1 cut(s) 513
FauI CCCGC 1 cut(s) 209
FauNDI CATATG 2 cut(s) 114, 403
FblI GTMKAC 1 cut(s) 483
Fnu4HI GCNGC 2 cut(s) 408, 520
FokI GGATG 2 cut(s) 278, 344
Fsp4HI GCNGC 2 cut(s) 408, 520
FspBI CTAG 2 cut(s) 41, 281
GlaI GCGC 1 cut(s) 497
GluI GCNGC 2 cut(s) 408, 520
HaeIII GGCC 2 cut(s) 62, 359
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 24
HhaI GCGC 1 cut(s) 498
Hin1II CATG 1 cut(s) 517
Hin6I GCGC 1 cut(s) 496
HinP1I GCGC 1 cut(s) 496
HinfI GANTC 6 cut(s) 151, 287, 306, 314, 485, 552
HpaII CCGG 1 cut(s) 93
HphI GGTGA 2 cut(s) 425, 445
Hpy166II GTNNAC 3 cut(s) 277, 475, 484
Hpy188I TCNGA 6 cut(s) 162, 169, 225, 346, 415, 444
Hpy188III TCNNGA 2 cut(s) 83, 503
Hpy8I GTNNAC 3 cut(s) 277, 475, 484
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 1 cut(s) 49
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 2 cut(s) 269, 519
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 528
HpyF3I CTNAG 3 cut(s) 153, 168, 567
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 1 cut(s) 517
HspAI GCGC 1 cut(s) 496
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
Kzo9I GATC 1 cut(s) 66
LmnI GCTCC 1 cut(s) 325
LpnPI CCDG 4 cut(s) 106, 314, 365, 444
Lsp1109I GCAGC 1 cut(s) 531
LweI GCATC 4 cut(s) 229, 256, 322, 419
MaeI CTAG 2 cut(s) 41, 281
MaeII ACGT 1 cut(s) 492
MalI GATC 1 cut(s) 68
MboI GATC 1 cut(s) 66
MboII GAAGA 2 cut(s) 428, 431
MflI RGATCY 1 cut(s) 66
MlsI TGGCCA 1 cut(s) 359
MluNI TGGCCA 1 cut(s) 359
MlyI GAGTC 2 cut(s) 145, 479
MmeI TCCRAC 1 cut(s) 185
MnlI CCTC 5 cut(s) 52, 163, 343, 350, 510
Mox20I TGGCCA 1 cut(s) 359
MroNI GCCGGC 1 cut(s) 92
MroXI GAANNNNTTC 1 cut(s) 449
MscI TGGCCA 1 cut(s) 359
MseI TTAA 2 cut(s) 120, 176
Msp20I TGGCCA 1 cut(s) 359
MspI CCGG 1 cut(s) 93
MvnI CGCG 2 cut(s) 35, 311
MwoI GCNNNNNNNGC 2 cut(s) 115, 528
NaeI GCCGGC 1 cut(s) 94
NdeI CATATG 2 cut(s) 114, 403
NdeII GATC 1 cut(s) 66
NgoMIV GCCGGC 1 cut(s) 92
NlaIII CATG 1 cut(s) 517
PdiI GCCGGC 1 cut(s) 94
PdmI GAANNNNTTC 1 cut(s) 449
PfeI GAWTC 4 cut(s) 287, 306, 314, 552
PkrI GCNGC 2 cut(s) 409, 521
PleI GAGTC 2 cut(s) 145, 479
PpsI GAGTC 2 cut(s) 145, 479
PsuI RGATCY 1 cut(s) 66
RsaI GTAC 2 cut(s) 366, 572
RsaNI GTAC 2 cut(s) 365, 571
SaqAI TTAA 2 cut(s) 120, 176
SatI GCNGC 2 cut(s) 408, 520
Sau3AI GATC 1 cut(s) 66
SchI GAGTC 2 cut(s) 145, 479
SetI ASST 6 cut(s) 107, 264, 282, 330, 438, 495
SfaNI GCATC 4 cut(s) 229, 256, 322, 419
SsiI CCGC 3 cut(s) 216, 311, 407
SspMI CTAG 2 cut(s) 41, 281
StyI CCWWGG 2 cut(s) 258, 436
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 1 cut(s) 495
TaqI TCGA 4 cut(s) 12, 84, 488, 504
TatI WGTACW 1 cut(s) 570
TauI GCSGC 1 cut(s) 410
TfiI GAWTC 4 cut(s) 287, 306, 314, 552
Tru1I TTAA 2 cut(s) 120, 176
Tru9I TTAA 2 cut(s) 120, 176
TscAI CASTG 1 cut(s) 367
TseI GCWGC 1 cut(s) 519
TspDTI ATGAA 2 cut(s) 101, 502
TspRI CASTG 1 cut(s) 367
XmiI GTMKAC 1 cut(s) 483
XmnI GAANNNNTTC 1 cut(s) 449
XspI CTAG 2 cut(s) 41, 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.