Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0013274

Physical Location & Seq

Genomic Coordinates Reverse (-)

23545356 .. 23547458

Gene Structure 2103 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1147100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2103 bp

ATGAATCCCAGCGGAGAAGCAGCAGCACCGCCGCCTCCAACGCTACAACCGCCGCAGCCGCCTCAGGCCCAACGACCCTCTACATCCTGCGCCCGACACCCGGAAGAGCATTTCACGGGCTTCTGCCCCTCATGCCTCTGCGAACGCCTTGCCGTGTTGGAACCTTCAACGACCTCTTCTTCCTCTTCAACTTCTCGAAAGCCCCCTACTTCCTCCACCGCCGCAGCCGCGCTTAAGGCCATTTTCAAGCCCCCCATTGGCGGTGGTGGGGCCTCCTCTTCCAATAAAAATCGACCCACCTCATTTTTCCCGGAGCTCCGACGAACCAAGTCTTTCTCTGCCTCTAAAAACGAGGGCTTCTCCGGCGTGTTTGAGCCGCAGAGGAAATCCTGCGACGTTCGGAACACTCTGTGGACACTCTTTCACCAAGACGACGGCCGGAATCCCAACAAGAAGGAAGCACCGCCCACTGACGTTGAGGTCGAGACCCGGAATTTGGGTGGGTCTTCTTCCAGCTTCCGGGGTCCGGTCTTTGAGGCCAAGGAGGAAGAGGAAGAGGAAGATAAAGCCGAAGAGGACGAGAACCAGTTTAGTGGCGGCGAGGACTTCGGAATTGAAATTTGTGAGGATGCAATTGTCCCAAATGTAACAGAAGACAGAGTTCCTGAAATCTTAGAGGAGGAGGAAGACTTTCAACCACCGCAGCCAGTGCCGGAGCAATTCGAAGAAGAGGAGTTGAAACCAATGAAAGATCACATAGATCTTGATTCTCAGACGAAGAAGCGAGACCTTAAGGAGATTGCGGGAAGCTTCTGGTCAGCAGCTTCGGTTTTCAGCAAGAAATTACAGAAATGGAGACAGAAACAGAAGCTGAAGAAGCGGCGGAACGGCGGCGGATCGGCCACATTGCCGGTGGAGAAGCCAATCGGGAGACAGTTCAGAGAAACTCAGTCGGAGATTGCCGATTACGGCTTCGGCCGGCGCTCCTGCGACACGGATCCGAGGTTTTCGCTAGACGTGGGTCGGATGTCGCTGGACGCGGGTCGGATGTCGTTCGACGACCCGAGATACTCCTTTGACGAGCCTCGGGCCTCATGGGACGGGTACCTCATCGGAAGAACGTTTCCGAGGATGCCAACGATGCTCTCGGTGGTGGAGGACGCTCCGGTGGTCCACGTGTCGAGATGCGACGCTGAGATTCCGGTGGAGGAGCCGCCCAAGAATTCGATTGAAGAGGAGACGGTTCCAGGCGGTTCGGCCCAGACCCGGGACTACTATTCGGACTCCTCTTCTCGGCGGAGGAAGAGCCTCGACCGATCCAACTCCATTAGGAAGACTGCGGCGGCGGTGGTGGCGGAGATTGATGAAATGAAGTCGGTTTCGAATGCCAATGCCAACGCCAAGGTGTCGCCCACGACTGTTCCTGATAACAGAGATCTGAGGGAATTTTACAACTCGAATTCTCTGAGGGATGACTGTTCTGAGAGCTTTGATATGGCGTCGTTTCGGGGCGGCGGAGATACGGGTTCGGTGGTGGGAAATGGGGAGAGGAATAAAGGGTGTAAAAAGTCTAGGAGGTGGGGCAAGGCGTGGAATATATGGAGTTTTATACACAGGAGGGGTGGGAACAAAGATGAGGACGATGATAGGTATAGTAGTGTTAGATCAAACGGCGTGGAGCGGTCTTATTCGGAATCGTGGCCGGAGCTCAGAGGCGGCAGCGGTGGTGGTGAGAGGAATGGGGAAGCCAAGGGCTTTAACCCAAAGATTATGAGGAGTAATAGTAGTGCTAGTTGGAGGAATGGTTCTCATGGGTATGGCGGTTTCGGTGGCGGTGCATTTGGGAGCATGAGGAAGAGCAATGGCGTTCCTGTCGTCGACGCAAATGGGTTTGGAAATGGGACTGCCGGGAGGAAGAAAAAAGATGATCAGGTTGTGCTGGAACGGAACCGGAGCGCGAGGTATTCCCCGAACCATATTGAAAACGGAATGCTGAGGTTTTATTTGACTCCGATGAGGAGCAGCTGGAGGAGCGGCGGAGTCGGGAAAAGCAGGTCCAGCCATGCTCATTCGATCGCCAGAAGCGTACTGAAACTGTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009965 GO:0010016 GO:0010051 GO:0010087 GO:0010088 GO:0010154 GO:0010232 GO:0010233 GO:0010588 GO:0016020 GO:0016324 GO:0022414 GO:0022622 GO:0032501 GO:0032502 GO:0044425 GO:0044459 GO:0044464 GO:0045177 GO:0048316 GO:0048366 GO:0048367 GO:0048598 GO:0048608 GO:0048731 GO:0048825 GO:0048826 GO:0048827 GO:0048856 GO:0051179 GO:0051234 GO:0061458 GO:0071944 GO:0090698 GO:0098590 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF740

Protein Analysis

701

Amino Acids

77.31

Weight (kDa)

7.55

Isoelectric Point (pI)

68.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF740	PF05340	17 - 676	8.2e-257	Protein of unknown function (DUF740)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013274)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38070
fragaria_vesca	FvH4_6g12000
malus_domestica	MD04G1147100.v1.1 MD12G1160400.v1.1
prunus_persica	Prupe.6G270900_v2.0.a1
pyrus_communis	pycom04g13290 pycom12g15220
rosa_chinensis	RchiOBHm_Chr3g0463831
rosa_laevigata	RLG00000024753
rosa_multiflora	Rmu_sc0004796.1_g000009
rosa_roxburghii	Rroxscaffold_6G00399380 Rroxscaffold_6G00416260
rosa_rugosa	Rorug03G0068100
rosa_samantha	Rh3AG125200 Rh3BG129500 Rh3CG132000 Rh3DG130500
rosa_wichuraiana	Rw3G010580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	479
Acc36I	ACCTGC	1 cut(s)	2043
Acc65I	GGTACC	1 cut(s)	1104
AccB1I	GGYRCC	1 cut(s)	1104
AccBSI	CCGCTC	2 cut(s)	1681, 2034
AccI	GTMKAC	1 cut(s)	1878
AccII	CGCG	3 cut(s)	230, 1040, 1958
AclI	AACGTT	1 cut(s)	1121
AclWI	GGATC	4 cut(s)	904, 992, 1005, 1311
AcoI	YGGCCR	4 cut(s)	436, 900, 976, 1700
AcsI	RAATTY	5 cut(s)	493, 618, 1222, 1445, 1459
AcuI	CTGAAG	1 cut(s)	893
AcvI	CACGTG	1 cut(s)	1177
AcyI	GRCGYC	1 cut(s)	1499
AdeI	CACNNNGTG	1 cut(s)	411
AfaI	GTAC	2 cut(s)	1106, 2088
AfiI	CCNNNNNNNGG	9 cut(s)	100, 257, 260, 496, 519, 526, 1266, 1267, 1293
AflII	CTTAAG	2 cut(s)	233, 791
AflIII	ACRYGT	1 cut(s)	1176
AgsI	TTSAA	8 cut(s)	168, 189, 247, 617, 695, 739, 1232, 1982
AjiI	CACGTC	1 cut(s)	1018
AjnI	CCWGG	1 cut(s)	1246
AloI	GAACNNNNNNTCC	2 cut(s)	1789, 1821
AluBI	AGCT	8 cut(s)	316, 516, 810, 824, 871, 1488, 1708, 2025
AluI	AGCT	8 cut(s)	316, 516, 810, 824, 871, 1488, 1708, 2025
Alw21I	GWGCWC	2 cut(s)	318, 1710
Alw26I	GTCTC	5 cut(s)	479, 780, 850, 925, 1232
AlwI	GGATC	4 cut(s)	904, 992, 1005, 1311
AlwNI	CAGNNNCTG	1 cut(s)	871
Ama87I	CYCGRG	3 cut(s)	1063, 1086, 1266
ApeKI	GCWGC	8 cut(s)	20, 23, 55, 224, 703, 821, 1719, 2022
ApoI	RAATTY	5 cut(s)	493, 618, 1222, 1445, 1459
ArsI	GACNNNNNNTTYG	2 cut(s)	478, 510
Asp700I	GAANNNNTTC	1 cut(s)	690
Asp718I	GGTACC	1 cut(s)	1104
AspLEI	GCGC	4 cut(s)	92, 232, 984, 1958
AspS9I	GGNCC	7 cut(s)	67, 270, 524, 1089, 1171, 1258, 2055
AsuC2I	CCSGG	7 cut(s)	101, 311, 490, 521, 1267, 1268, 1909
AsuHPI	GGTGA	2 cut(s)	416, 1742
AsuII	TTCGAA	2 cut(s)	723, 1382
AvaI	CYCGRG	3 cut(s)	1063, 1086, 1266
AvaII	GGWCC	3 cut(s)	524, 1171, 2055
AxyI	CCTNAGG	1 cut(s)	63
BamHI	GGATCC	1 cut(s)	997
BanI	GGYRCC	1 cut(s)	1104
BanII	GRGCYC	2 cut(s)	318, 1710
BbrPI	CACGTG	1 cut(s)	1177
BbsI	GAAGAC	4 cut(s)	498, 660, 693, 1340
Bbv12I	GWGCWC	2 cut(s)	318, 1710
BbvCI	CCTCAGC	1 cut(s)	1994
BbvI	GCAGC	8 cut(s)	32, 35, 67, 236, 715, 833, 1731, 2034
BceAI	ACGGC	5 cut(s)	137, 451, 904, 985, 1687
BcgI	CGANNNNNNTGC	2 cut(s)	131, 165
BciT130I	CCWGG	1 cut(s)	1248
BclI	TGATCA	1 cut(s)	1927
BcnI	CCSGG	7 cut(s)	101, 311, 490, 521, 1267, 1268, 1909
BcoDI	GTCTC	5 cut(s)	479, 780, 850, 925, 1232
BfaI	CTAG	3 cut(s)	1013, 1572, 1791
BfoI	RGCGCY	1 cut(s)	985
BfrI	CTTAAG	2 cut(s)	233, 791
BfuAI	ACCTGC	1 cut(s)	2043
BglII	AGATCT	2 cut(s)	760, 1435
Bme1390I	CCNGG	8 cut(s)	101, 311, 490, 521, 1248, 1267, 1268, 1909
Bme18I	GGWCC	3 cut(s)	524, 1171, 2055
BmeT110I	CYCGRG	3 cut(s)	1063, 1086, 1266
BmgBI	CACGTC	1 cut(s)	1018
BmgT120I	GGNCC	7 cut(s)	67, 270, 524, 1089, 1171, 1258, 2055
BmiI	GGNNCC	8 cut(s)	162, 271, 525, 999, 1106, 1212, 1245, 1949
BmrFI	CCNGG	8 cut(s)	101, 311, 490, 521, 1248, 1267, 1268, 1909
BmsI	GCATC	4 cut(s)	619, 1122, 1131, 1175
BoxI	GACNNNNGTC	2 cut(s)	1020, 1041
BpiI	GAAGAC	4 cut(s)	498, 660, 693, 1340
BplI	GAGNNNNNCTC	2 cut(s)	344, 376
BpmI	CTGGAG	1 cut(s)	2047
Bpu10I	CCTNAGC	1 cut(s)	1994
Bpu14I	TTCGAA	2 cut(s)	723, 1382
BpuMI	CCSGG	7 cut(s)	101, 311, 490, 521, 1267, 1268, 1909
BsaAI	YACGTR	1 cut(s)	1177
BsaHI	GRCGYC	1 cut(s)	1499
BsaI	GGTCTC	2 cut(s)	479, 780
BsaJI	CCNNGG	8 cut(s)	520, 540, 1001, 1085, 1127, 1266, 1401, 1749
BsaWI	WCCGGW	4 cut(s)	526, 1165, 1201, 1950
BsaXI	ACNNNNNCTCC	4 cut(s)	1768, 1789, 1798, 1819
Bsc4I	CCNNNNNNNGG	9 cut(s)	100, 257, 260, 496, 519, 526, 1266, 1267, 1293
Bse118I	RCCGGY	2 cut(s)	910, 978
Bse1I	ACTGG	2 cut(s)	586, 707
Bse21I	CCTNAGG	1 cut(s)	63
Bse3DI	GCAATG	2 cut(s)	905, 1867
BseBI	CCWGG	1 cut(s)	1248
BseDI	CCNNGG	8 cut(s)	520, 540, 1001, 1085, 1127, 1266, 1401, 1749
BseGI	GGATG	6 cut(s)	83, 634, 1032, 1053, 1137, 1477
BseLI	CCNNNNNNNGG	9 cut(s)	100, 257, 260, 496, 519, 526, 1266, 1267, 1293
BseMI	GCAATG	2 cut(s)	905, 1867
BseMII	CTCAG	9 cut(s)	77, 785, 962, 1185, 1430, 1457, 1473, 1723, 1985
BseNI	ACTGG	2 cut(s)	586, 707
BseX3I	CGGCCG	2 cut(s)	436, 976
BseXI	GCAGC	8 cut(s)	32, 35, 67, 236, 715, 833, 1731, 2034
BseYI	CCCAGC	1 cut(s)	8
Bsh1236I	CGCG	3 cut(s)	230, 1040, 1958
Bsh1285I	CGRYCG	4 cut(s)	439, 979, 1315, 2076
BshNI	GGYRCC	1 cut(s)	1104
BsiEI	CGRYCG	4 cut(s)	439, 979, 1315, 2076
BsiHKAI	GWGCWC	2 cut(s)	318, 1710
BsiHKCI	CYCGRG	3 cut(s)	1063, 1086, 1266
BslFI	GGGAC	4 cut(s)	623, 1112, 1283, 1915
BslI	CCNNNNNNNGG	9 cut(s)	100, 257, 260, 496, 519, 526, 1266, 1267, 1293
BsmAI	GTCTC	5 cut(s)	479, 780, 850, 925, 1232
BsmBI	CGTCTC	1 cut(s)	1232
BsmFI	GGGAC	4 cut(s)	623, 1112, 1283, 1915
BsmI	GAATGC	2 cut(s)	1390, 1995
Bso31I	GGTCTC	2 cut(s)	479, 780
BsoBI	CYCGRG	3 cut(s)	1063, 1086, 1266
Bsp119I	TTCGAA	2 cut(s)	723, 1382
Bsp1286I	GDGCHC	2 cut(s)	318, 1710
Bsp143I	GATC	9 cut(s)	751, 760, 896, 997, 1316, 1435, 1664, 1927, 2073
BspCNI	CTCAG	9 cut(s)	76, 784, 961, 1186, 1431, 1458, 1474, 1722, 1986
BspFNI	CGCG	3 cut(s)	230, 1040, 1958
BspLI	GGNNCC	8 cut(s)	162, 271, 525, 999, 1106, 1212, 1245, 1949
BspMI	ACCTGC	1 cut(s)	2043
BspPI	GGATC	4 cut(s)	904, 992, 1005, 1311
BspQI	GCTCTTC	3 cut(s)	99, 1298, 1850
BspT104I	TTCGAA	2 cut(s)	723, 1382
BspT107I	GGYRCC	1 cut(s)	1104
BspTI	CTTAAG	2 cut(s)	233, 791
BspTNI	GGTCTC	2 cut(s)	479, 780
BsrBI	CCGCTC	2 cut(s)	1681, 2034
BsrDI	GCAATG	2 cut(s)	905, 1867
BsrFI	RCCGGY	2 cut(s)	910, 978
BsrI	ACTGG	2 cut(s)	586, 707
BssAI	RCCGGY	2 cut(s)	910, 978
BssECI	CCNNGG	8 cut(s)	520, 540, 1001, 1085, 1127, 1266, 1401, 1749
BssMI	GATC	9 cut(s)	751, 760, 896, 997, 1316, 1435, 1664, 1927, 2073
BssNI	GRCGYC	1 cut(s)	1499
BssT1I	CCWWGG	3 cut(s)	540, 1401, 1749
Bst2UI	CCWGG	1 cut(s)	1248
Bst4CI	ACNGT	5 cut(s)	936, 1243, 1420, 1478, 2097
BstACI	GRCGYC	1 cut(s)	1499
BstAFI	CTTAAG	2 cut(s)	233, 791
BstAPI	GCANNNNNTGC	1 cut(s)	709
BstBAI	YACGTR	1 cut(s)	1177
BstBI	TTCGAA	2 cut(s)	723, 1382
BstC8I	GCNNGC	1 cut(s)	980
BstF5I	GGATG	6 cut(s)	83, 634, 1032, 1053, 1137, 1477
BstFNI	CGCG	3 cut(s)	230, 1040, 1958
BstH2I	RGCGCY	1 cut(s)	985
BstHHI	GCGC	4 cut(s)	92, 232, 984, 1958
BstKTI	GATC	9 cut(s)	754, 763, 899, 1000, 1319, 1438, 1667, 1930, 2076
BstMAI	GTCTC	5 cut(s)	479, 780, 850, 925, 1232
BstMBI	GATC	9 cut(s)	751, 760, 896, 997, 1316, 1435, 1664, 1927, 2073
BstMCI	CGRYCG	4 cut(s)	439, 979, 1315, 2076
BstNI	CCWGG	1 cut(s)	1248
BstPAI	GACNNNNGTC	2 cut(s)	1020, 1041
BstSCI	CCNGG	8 cut(s)	99, 309, 488, 519, 1246, 1265, 1266, 1907
BstUI	CGCG	3 cut(s)	230, 1040, 1958
BstV1I	GCAGC	8 cut(s)	32, 35, 67, 236, 715, 833, 1731, 2034
BstV2I	GAAGAC	4 cut(s)	498, 660, 693, 1340
BstX2I	RGATCY	3 cut(s)	760, 997, 1435
BstXI	CCANNNNNNTGG	1 cut(s)	593
BstYI	RGATCY	3 cut(s)	760, 997, 1435
BstZI	CGGCCG	2 cut(s)	436, 976
Bsu36I	CCTNAGG	1 cut(s)	63
BtrI	CACGTC	1 cut(s)	1018
BtsCI	GGATG	6 cut(s)	83, 634, 1032, 1053, 1137, 1477
BtsIMutI	CAGTG	2 cut(s)	468, 714
BveI	ACCTGC	1 cut(s)	2043
Cac8I	GCNNGC	1 cut(s)	980
CaiI	CAGNNNCTG	1 cut(s)	871
CfoI	GCGC	4 cut(s)	92, 232, 984, 1958
Cfr10I	RCCGGY	2 cut(s)	910, 978
Cfr13I	GGNCC	7 cut(s)	67, 270, 524, 1089, 1171, 1258, 2055
Cfr9I	CCCGGG	1 cut(s)	1266
CseI	GACGC	5 cut(s)	1046, 1169, 1199, 1488, 1889
Csp6I	GTAC	2 cut(s)	1105, 2087
CspCI	CAANNNNNGTGG	4 cut(s)	457, 492, 1656, 1691
CviAII	CATG	5 cut(s)	132, 1095, 1811, 1849, 2063
CviQI	GTAC	2 cut(s)	1105, 2087
DpnI	GATC	9 cut(s)	753, 762, 898, 999, 1318, 1437, 1666, 1929, 2075
DpnII	GATC	9 cut(s)	751, 760, 896, 997, 1316, 1435, 1664, 1927, 2073
DraIII	CACNNNGTG	1 cut(s)	411
DrdI	GACNNNNNNGTC	1 cut(s)	479
DseDI	GACNNNNNNGTC	1 cut(s)	479
EaeI	YGGCCR	4 cut(s)	436, 900, 976, 1700
EagI	CGGCCG	2 cut(s)	436, 976
EciI	GGCGGA	6 cut(s)	898, 909, 1312, 1370, 1530, 2052
Ecl136II	GAGCTC	2 cut(s)	316, 1708
EclXI	CGGCCG	2 cut(s)	436, 976
Eco130I	CCWWGG	3 cut(s)	540, 1401, 1749
Eco24I	GRGCYC	2 cut(s)	318, 1710
Eco31I	GGTCTC	2 cut(s)	479, 780
Eco47I	GGWCC	3 cut(s)	524, 1171, 2055
Eco52I	CGGCCG	2 cut(s)	436, 976
Eco53kI	GAGCTC	2 cut(s)	316, 1708
Eco57I	CTGAAG	1 cut(s)	893
Eco72I	CACGTG	1 cut(s)	1177
Eco81I	CCTNAGG	1 cut(s)	63
Eco88I	CYCGRG	3 cut(s)	1063, 1086, 1266
EcoICRI	GAGCTC	2 cut(s)	316, 1708
EcoO109I	RGGNCCY	1 cut(s)	270
EcoRI	GAATTC	2 cut(s)	1222, 1459
EcoRII	CCWGG	1 cut(s)	1246
EcoT14I	CCWWGG	3 cut(s)	540, 1401, 1749
EcoT38I	GRGCYC	2 cut(s)	318, 1710
ErhI	CCWWGG	3 cut(s)	540, 1401, 1749
Esp3I	CGTCTC	1 cut(s)	1232
FaeI	CATG	5 cut(s)	135, 1098, 1814, 1852, 2066
FaqI	GGGAC	4 cut(s)	623, 1112, 1283, 1915
FatI	CATG	5 cut(s)	131, 1094, 1810, 1848, 2062
FauI	CCCGC	2 cut(s)	796, 1033
FbaI	TGATCA	1 cut(s)	1927
FblI	GTMKAC	1 cut(s)	1878
FokI	GGATG	6 cut(s)	70, 641, 1039, 1060, 1144, 1484
FriOI	GRGCYC	2 cut(s)	318, 1710
FspBI	CTAG	3 cut(s)	1013, 1572, 1791
GlaI	GCGC	4 cut(s)	91, 231, 983, 1957
GsaI	CCCAGC	1 cut(s)	12
GsuI	CTGGAG	1 cut(s)	2047
HaeII	RGCGCY	1 cut(s)	985
HgaI	GACGC	5 cut(s)	1046, 1169, 1199, 1488, 1889
HhaI	GCGC	4 cut(s)	92, 232, 984, 1958
Hin1I	GRCGYC	1 cut(s)	1499
Hin1II	CATG	5 cut(s)	135, 1098, 1814, 1852, 2066
Hin6I	GCGC	4 cut(s)	90, 230, 982, 1956
HinP1I	GCGC	4 cut(s)	90, 230, 982, 1956
HincII	GTYRAC	1 cut(s)	1879
HindII	GTYRAC	1 cut(s)	1879
HindIII	AAGCTT	1 cut(s)	808
HinfI	GANTC	8 cut(s)	4, 442, 767, 1198, 1283, 1694, 2008, 2040
HphI	GGTGA	2 cut(s)	416, 1742
Hpy166II	GTNNAC	3 cut(s)	414, 1174, 1879
Hpy188III	TCNNGA	8 cut(s)	195, 484, 665, 764, 928, 1182, 1424, 2044
Hpy8I	GTNNAC	3 cut(s)	414, 1174, 1879
Hpy99I	CGWCG	8 cut(s)	324, 398, 437, 1061, 1193, 1504, 1880, 1883
HpyAV	CCTTC	2 cut(s)	174, 448
HpyCH4III	ACNGT	5 cut(s)	936, 1243, 1420, 1478, 2097
HpyCH4IV	ACGT	5 cut(s)	396, 474, 1017, 1121, 1176
HpyCH4V	TGCA	2 cut(s)	632, 1838
HpySE526I	ACGT	5 cut(s)	396, 474, 1017, 1121, 1176
Hsp92I	GRCGYC	1 cut(s)	1499
Hsp92II	CATG	5 cut(s)	135, 1098, 1814, 1852, 2066
HspAI	GCGC	4 cut(s)	90, 230, 982, 1956
KpnI	GGTACC	1 cut(s)	1108
KroI	GCCGGC	1 cut(s)	978
KroNI	GCCGGC	1 cut(s)	980
Ksp22I	TGATCA	1 cut(s)	1927
Kzo9I	GATC	9 cut(s)	751, 760, 896, 997, 1316, 1435, 1664, 1927, 2073
LguI	GCTCTTC	3 cut(s)	99, 1298, 1850
Lsp1109I	GCAGC	8 cut(s)	32, 35, 67, 236, 715, 833, 1731, 2034
LweI	GCATC	4 cut(s)	619, 1122, 1131, 1175
MaeI	CTAG	3 cut(s)	1013, 1572, 1791
MaeII	ACGT	5 cut(s)	396, 474, 1017, 1121, 1176
MaeIII	GTNAC	1 cut(s)	646
MalI	GATC	9 cut(s)	753, 762, 898, 999, 1318, 1437, 1666, 1929, 2075
MbiI	CCGCTC	2 cut(s)	1681, 2034
MboI	GATC	9 cut(s)	751, 760, 896, 997, 1316, 1435, 1664, 1927, 2073
MfeI	CAATTG	1 cut(s)	633
MflI	RGATCY	3 cut(s)	760, 997, 1435
MhlI	GDGCHC	2 cut(s)	318, 1710
MluCI	AATT	9 cut(s)	493, 612, 618, 633, 719, 842, 1222, 1445, 1459
MlyI	GAGTC	3 cut(s)	1277, 2002, 2049
MmeI	TCCRAC	8 cut(s)	62, 138, 343, 933, 1004, 1025, 1344, 1775
MroNI	GCCGGC	1 cut(s)	978
MroXI	GAANNNNTTC	1 cut(s)	690
MseI	TTAA	3 cut(s)	234, 792, 1758
MslI	CAYNNNNRTG	1 cut(s)	1815
MspA1I	CMGCKG	3 cut(s)	12, 1722, 2025
MspCI	CTTAAG	2 cut(s)	233, 791
MspR9I	CCNGG	8 cut(s)	101, 311, 490, 521, 1248, 1267, 1268, 1909
MunI	CAATTG	1 cut(s)	633
Mva1269I	GAATGC	2 cut(s)	1390, 1995
MvaI	CCWGG	1 cut(s)	1248
MvnI	CGCG	3 cut(s)	230, 1040, 1958
NaeI	GCCGGC	1 cut(s)	980
NciI	CCSGG	7 cut(s)	101, 311, 490, 521, 1267, 1268, 1909
NdeII	GATC	9 cut(s)	751, 760, 896, 997, 1316, 1435, 1664, 1927, 2073
NgoMIV	GCCGGC	1 cut(s)	978
NlaIII	CATG	5 cut(s)	135, 1098, 1814, 1852, 2066
NlaIV	GGNNCC	8 cut(s)	162, 271, 525, 999, 1106, 1212, 1245, 1949
NmeAIII	GCCGAG	1 cut(s)	1273
NspV	TTCGAA	2 cut(s)	723, 1382
PciSI	GCTCTTC	3 cut(s)	99, 1298, 1850
PcsI	WCGNNNNNNNCGW	2 cut(s)	480, 2082
PctI	GAATGC	2 cut(s)	1390, 1995
PdiI	GCCGGC	1 cut(s)	980
PdmI	GAANNNNTTC	1 cut(s)	690
PfeI	GAWTC	5 cut(s)	4, 442, 767, 1198, 1694
PfoI	TCCNGGA	1 cut(s)	309
Ple19I	CGATCG	1 cut(s)	2076
PleI	GAGTC	3 cut(s)	1277, 2002, 2048
PmaCI	CACGTG	1 cut(s)	1177
PmlI	CACGTG	1 cut(s)	1177
PpsI	GAGTC	3 cut(s)	1277, 2002, 2048
Ppu21I	YACGTR	1 cut(s)	1177
PshAI	GACNNNNGTC	2 cut(s)	1020, 1041
Psp124BI	GAGCTC	2 cut(s)	318, 1710
Psp1406I	AACGTT	1 cut(s)	1121
Psp6I	CCWGG	1 cut(s)	1246
PspCI	CACGTG	1 cut(s)	1177
PspFI	CCCAGC	1 cut(s)	8
PspGI	CCWGG	1 cut(s)	1246
PspN4I	GGNNCC	8 cut(s)	162, 271, 525, 999, 1106, 1212, 1245, 1949
PspPI	GGNCC	7 cut(s)	67, 270, 524, 1089, 1171, 1258, 2055
PstNI	CAGNNNCTG	1 cut(s)	871
PsuI	RGATCY	3 cut(s)	760, 997, 1435
PvuI	CGATCG	1 cut(s)	2076
PvuII	CAGCTG	1 cut(s)	2025
RsaI	GTAC	2 cut(s)	1106, 2088
RsaNI	GTAC	2 cut(s)	1105, 2087
RseI	CAYNNNNRTG	1 cut(s)	1815
SacI	GAGCTC	2 cut(s)	318, 1710
SalI	GTCGAC	1 cut(s)	1877
SapI	GCTCTTC	3 cut(s)	99, 1298, 1850
SaqAI	TTAA	3 cut(s)	234, 792, 1758
Sau3AI	GATC	9 cut(s)	751, 760, 896, 997, 1316, 1435, 1664, 1927, 2073
Sau96I	GGNCC	7 cut(s)	67, 270, 524, 1089, 1171, 1258, 2055
SchI	GAGTC	3 cut(s)	1277, 2002, 2049
ScrFI	CCNGG	8 cut(s)	101, 311, 490, 521, 1248, 1267, 1268, 1909
SduI	GDGCHC	2 cut(s)	318, 1710
SfaNI	GCATC	4 cut(s)	619, 1122, 1131, 1175
SfuI	TTCGAA	2 cut(s)	723, 1382
SgrDI	CGTCGACG	1 cut(s)	1877
SinI	GGWCC	3 cut(s)	524, 1171, 2055
SmaI	CCCGGG	1 cut(s)	1268
SmiMI	CAYNNNNRTG	1 cut(s)	1815
SmlI	CTYRAG	2 cut(s)	233, 791
SmoI	CTYRAG	2 cut(s)	233, 791
Sse9I	AATT	9 cut(s)	493, 612, 618, 633, 719, 842, 1222, 1445, 1459
SspMI	CTAG	3 cut(s)	1013, 1572, 1791
SstI	GAGCTC	2 cut(s)	318, 1710
StyD4I	CCNGG	8 cut(s)	99, 309, 488, 519, 1246, 1265, 1266, 1907
StyI	CCWWGG	3 cut(s)	540, 1401, 1749
TaaI	ACNGT	5 cut(s)	936, 1243, 1420, 1478, 2097
TaiI	ACGT	5 cut(s)	399, 477, 1020, 1124, 1179
TaqII	GACCGA	1 cut(s)	1329
TasI	AATT	9 cut(s)	493, 612, 618, 633, 719, 842, 1222, 1445, 1459
TfiI	GAWTC	5 cut(s)	4, 442, 767, 1198, 1694
Tru1I	TTAA	3 cut(s)	234, 792, 1758
Tru9I	TTAA	3 cut(s)	234, 792, 1758
TscAI	CASTG	2 cut(s)	475, 714
TseI	GCWGC	8 cut(s)	20, 23, 55, 224, 703, 821, 1719, 2022
TspDTI	ATGAA	4 cut(s)	17, 761, 1380, 1385
TspGWI	ACGGA	3 cut(s)	1010, 1960, 2001
TspMI	CCCGGG	1 cut(s)	1266
TspRI	CASTG	2 cut(s)	475, 714
Vha464I	CTTAAG	2 cut(s)	233, 791
VpaK11BI	GGWCC	3 cut(s)	524, 1171, 2055
XapI	RAATTY	5 cut(s)	493, 618, 1222, 1445, 1459
XcmI	CCANNNNNNNNNTGG	1 cut(s)	910
XmaI	CCCGGG	1 cut(s)	1266
XmiI	GTMKAC	1 cut(s)	1878
XmnI	GAANNNNTTC	1 cut(s)	690
XspI	CTAG	3 cut(s)	1013, 1572, 1791

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.