Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0013274

Physical Location & Seq

Genomic Coordinates Reverse (-)

17567733 .. 17569811

Gene Structure 2079 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g15220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2079 bp

ATGAATCCCAGCGCAGAAGCAGTTGCACCGCCGCCGCCACCGCTTCAACCGTCGCAGCTGCCTCAGGCCCACCGACTTTCCACCTCCTGCGATCGCCACCCGGAAGAGCATTTCACGGGCTTCTGCCCCTCATGCCTCTGCGAACGCCTTGCCGTGTTGGAGCCTTCAACAAACTCTTCTTCCTCTTCCACTTCTCGAAAACCCCCTACTTCCTCCAACGCCGCCGCCGCTCTTAGGGCCATTTTCAAGCCCCCCATTGGCGGTGGTGGGGCCTCCTCTTCCAATAAAAACCGACCCACCTCATTTTTCCCGGAGCTCCGAAGAACCAAGTCTTTCTCTGCCTCTAAAAACGAGGGCTTCTCCGGCGTGTTTGAGCCGCAGAGGAAGTCTTGCGACGTTAGGGTTCGGAACACTCTTTGGACACTCTTTCACCAGGACGACGGACGGAATCCTAACAAGAAGGAGGCACCGCCTACTGACGCTGAGGTTGAGACCCGAAATTTGGGTGGGTCTTCTTCCAGCTTTCGAGGTCCAGTCTTCGAGTCCAAGGAGGAAGACGAAGACGAAGAGGACGAAAATCAGTGCAGCGGCGGCGAAGACTTTGGAATTGAAATTTGCGAGGAAACAATTGTCGCCAATGTGACAGAAGACAAAGTTCCAGAAATCGTAGAGGAGGAGGAAGAAGAATTTCAACCACCGCAACCAGTCACGGAACAATTCGAAGAAGAGGACTTGAAGCCCATGAAAGATCATATAGATCTTGATTCACAGACAAAGAAGCGAGACCTGAAGGAGATTGCGGGCAGCTTCTGGTCAGCAGCTTCAGTTTTCAGCAAAAAATTACAGAAATGGAGGCAGAAGCAGAAGCTGAAGAAGCGGCGGAACGGCTGCGGATCGGCCACATTGCCGGTGGAGAAGCCAATCGGGAGACAGTTCAGAGAAACCCAGTCGGAGATCGCCGATTACGGCTTCGGCCGGCGTTCCTGCGACACGGATCCGAGGTTTTCGCTCGACGCGGGTCGGATGTCGTTCGACGACCCGAGGTACTCCTTTGACGAGCCTCGGGCCTCGTGGGACGGGTACCTCATCGGAAGGACGTTTCCGAGAATGCCCACGATGCTCTCGGTGGTGGAGGACGCTCCGGTGGTCCACGTGGCGAGATGCGACGCTCAAATTCCGGTTGAGGAGCCGCCCATGAATTCCATTGAGGAGGAGACGGTTCCGGGCGGGTCGGCCCAGACCCGGGACTATTATTTGGACTCGTCGTCTCGCCGGAGGAAGAGCCTCGACCGGTCCAACTCCATTAGGAAGACTGCGGAGGCGGTGGTGGCAGATATTGATGAGATGAAGTCGGTTTCGAATGCCAATGGCAATGCCAAGGTGTCGCCCACGACTGTTCCGGATAACAGAGATTTGAGGGAATTTTACAACTCGAATTCTTTGAGGGATGACTGCTCGGAGACCTTTGAAACGGGGTCGTTTCGGGGCGGCGGCGATTCGACATCGGTGGTGGGAAATGGGGAGAGGAATAAAGGGTGTAAAAAGTCTAGGAGGTGGAGCAAGGCGTGGAATATATGGAGTTTTATACACAGGAGGGGTGGGAACAAAGATGAGGATGACGATAGGTATAGCAGTGTTAGAGCTAATGGGGTGGAGCGGTCTTTTTCGGAGTCGTGGCCGGAGCTCAGAGGCGGCGGTGGTGGCGAGAGGAATGGGGAAGCCAAGGGCTTTAATCCTAAGCTTATGAGGAGTAATAGTAGTGCTAGTTGGAGGAATGGTTCTCATGGGTATGGCGGTTTTGGTGGCGGTGCACTCGGGAGCATGAGGAAGAGCAATGGCAGCGTTCCTGTGGTCGAGGCAAACGGGTTTGGAAATGGGAACGGCGGAAGGAAGAAGAAAGACGACCAGGTTGTGCTGGAACGGAACCGGAGCGCGAGGTATTCCCCGAACCATATTGAGAACGGATTGCTGAGGTTTTATTTGACGCCGATGAGGAGCAGCTGGAGGAGCGGCGGAGTCGGGAAAAGCAGGTCCAGCCATGCTCATTCGATTGCCAGAAGCGTACTGAGATTGTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009965 GO:0010016 GO:0010051 GO:0010087 GO:0010088 GO:0010154 GO:0010232 GO:0010233 GO:0010588 GO:0016020 GO:0016324 GO:0022414 GO:0022622 GO:0032501 GO:0032502 GO:0044425 GO:0044459 GO:0044464 GO:0045177 GO:0048316 GO:0048366 GO:0048367 GO:0048598 GO:0048608 GO:0048731 GO:0048825 GO:0048826 GO:0048827 GO:0048856 GO:0051179 GO:0051234 GO:0061458 GO:0071944 GO:0090698 GO:0098590 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF740

Protein Analysis

693

Amino Acids

76.65

Weight (kDa)

7.16

Isoelectric Point (pI)

65.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF740	PF05340	17 - 668	2.6e-267	Protein of unknown function (DUF740)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013274)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38070
fragaria_vesca	FvH4_6g12000
malus_domestica	MD04G1147100.v1.1 MD12G1160400.v1.1
prunus_persica	Prupe.6G270900_v2.0.a1
pyrus_communis	pycom04g13290 pycom12g15220
rosa_chinensis	RchiOBHm_Chr3g0463831
rosa_laevigata	RLG00000024753
rosa_multiflora	Rmu_sc0004796.1_g000009
rosa_roxburghii	Rroxscaffold_6G00399380 Rroxscaffold_6G00416260
rosa_rugosa	Rorug03G0068100
rosa_samantha	Rh3AG125200 Rh3BG129500 Rh3CG132000 Rh3DG130500
rosa_wichuraiana	Rw3G010580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2019
Acc65I	GGTACC	1 cut(s)	1080
AccB1I	GGYRCC	2 cut(s)	466, 1080
AccBSI	CCGCTC	3 cut(s)	230, 1657, 2010
AccII	CGCG	2 cut(s)	1016, 1934
AccIII	TCCGGA	1 cut(s)	1399
AclWI	GGATC	3 cut(s)	901, 989, 1002
AcoI	YGGCCR	3 cut(s)	897, 973, 1676
AcsI	RAATTY	7 cut(s)	499, 612, 686, 1173, 1198, 1421, 1435
AcuI	CTGAAG	3 cut(s)	807, 809, 890
AcvI	CACGTG	1 cut(s)	1153
AcyI	GRCGYC	1 cut(s)	1985
AfaI	GTAC	4 cut(s)	1046, 1082, 2064, 2075
AfiI	CCNNNNNNNGG	6 cut(s)	234, 257, 260, 502, 1242, 1243
AgeI	ACCGGT	1 cut(s)	1290
AgsI	TTSAA	7 cut(s)	47, 168, 247, 611, 692, 736, 1469
AhdI	GACNNNNNGTC	1 cut(s)	1264
AjnI	CCWGG	2 cut(s)	432, 1905
AloI	GAACNNNNNNTCC	2 cut(s)	1762, 1794
Alw21I	GWGCWC	3 cut(s)	318, 1686, 1813
Alw26I	GTCTC	6 cut(s)	485, 777, 922, 1208, 1272, 1454
Alw44I	GTGCAC	1 cut(s)	1809
AlwI	GGATC	3 cut(s)	901, 989, 1002
AlwNI	CAGNNNCTG	2 cut(s)	810, 868
Ama87I	CYCGRG	4 cut(s)	1039, 1062, 1242, 1814
Aor13HI	TCCGGA	1 cut(s)	1399
AoxI	GGCC	8 cut(s)	66, 237, 270, 897, 973, 1065, 1233, 1676
ApaLI	GTGCAC	1 cut(s)	1809
ApeKI	GCWGC	8 cut(s)	55, 58, 585, 804, 818, 888, 1839, 1998
ApoI	RAATTY	7 cut(s)	499, 612, 686, 1173, 1198, 1421, 1435
ArsI	GACNNNNNNTTYG	2 cut(s)	484, 516
AsiGI	ACCGGT	1 cut(s)	1290
AsiSI	GCGATCGC	1 cut(s)	94
Asp700I	GAANNNNTTC	1 cut(s)	687
Asp718I	GGTACC	1 cut(s)	1080
AspLEI	GCGC	2 cut(s)	14, 1934
AspS9I	GGNCC	9 cut(s)	67, 237, 270, 530, 1065, 1147, 1234, 1293, 2031
AsuC2I	CCSGG	5 cut(s)	101, 311, 1224, 1243, 1244
AsuHPI	GGTGA	1 cut(s)	422
AsuII	TTCGAA	2 cut(s)	720, 1358
AvaI	CYCGRG	4 cut(s)	1039, 1062, 1242, 1814
AvaII	GGWCC	4 cut(s)	530, 1147, 1293, 2031
AxyI	CCTNAGG	1 cut(s)	63
BaeGI	GKGCMC	1 cut(s)	1813
BamHI	GGATCC	1 cut(s)	994
BanI	GGYRCC	2 cut(s)	466, 1080
BanII	GRGCYC	2 cut(s)	318, 1686
BauI	CACGAG	1 cut(s)	1069
BbrPI	CACGTG	1 cut(s)	1153
BbsI	GAAGAC	7 cut(s)	504, 529, 561, 567, 603, 654, 1316
Bbv12I	GWGCWC	3 cut(s)	318, 1686, 1813
BbvCI	CCTCAGC	2 cut(s)	483, 1970
BbvI	GCAGC	8 cut(s)	45, 67, 597, 816, 830, 875, 1851, 2010
BceAI	ACGGC	4 cut(s)	137, 901, 982, 1897
BcgI	CGANNNNNNTGC	2 cut(s)	131, 165
BciT130I	CCWGG	2 cut(s)	434, 1907
BcnI	CCSGG	5 cut(s)	101, 311, 1224, 1243, 1244
BcoDI	GTCTC	6 cut(s)	485, 777, 922, 1208, 1272, 1454
BfaI	CTAG	2 cut(s)	1548, 1764
BfuAI	ACCTGC	1 cut(s)	2019
BglII	AGATCT	1 cut(s)	757
Bme1390I	CCNGG	7 cut(s)	101, 311, 434, 1224, 1243, 1244, 1907
Bme18I	GGWCC	4 cut(s)	530, 1147, 1293, 2031
BmeRI	GACNNNNNGTC	1 cut(s)	1264
BmeT110I	CYCGRG	4 cut(s)	1039, 1062, 1242, 1814
BmgT120I	GGNCC	9 cut(s)	67, 237, 270, 530, 1065, 1147, 1234, 1293, 2031
BmiI	GGNNCC	8 cut(s)	162, 271, 468, 996, 1082, 1188, 1221, 1925
BmrFI	CCNGG	7 cut(s)	101, 311, 434, 1224, 1243, 1244, 1907
BmrI	ACTGGG	1 cut(s)	940
BmsI	GCATC	2 cut(s)	1107, 1151
BmuI	ACTGGG	1 cut(s)	940
BoxI	GACNNNNGTC	1 cut(s)	1017
BpiI	GAAGAC	7 cut(s)	504, 529, 561, 567, 603, 654, 1316
BplI	GAGNNNNNCTC	2 cut(s)	344, 376
BpmI	CTGGAG	1 cut(s)	2023
Bpu10I	CCTNAGC	3 cut(s)	483, 1737, 1970
Bpu14I	TTCGAA	2 cut(s)	720, 1358
BpuMI	CCSGG	5 cut(s)	101, 311, 1224, 1243, 1244
BsaAI	YACGTR	1 cut(s)	1153
BsaHI	GRCGYC	1 cut(s)	1985
BsaI	GGTCTC	3 cut(s)	485, 777, 1454
BsaJI	CCNNGG	7 cut(s)	546, 998, 1040, 1061, 1242, 1377, 1722
BsaWI	WCCGGW	5 cut(s)	1141, 1177, 1290, 1399, 1926
BsaXI	ACNNNNNCTCC	4 cut(s)	1741, 1762, 1771, 1792
Bsc4I	CCNNNNNNNGG	6 cut(s)	234, 257, 260, 502, 1242, 1243
Bse118I	RCCGGY	3 cut(s)	907, 975, 1290
Bse1I	ACTGG	3 cut(s)	533, 704, 946
Bse21I	CCTNAGG	1 cut(s)	63
Bse3DI	GCAATG	3 cut(s)	902, 1378, 1840
BseAI	TCCGGA	1 cut(s)	1399
BseBI	CCWGG	2 cut(s)	434, 1907
BseDI	CCNNGG	7 cut(s)	546, 998, 1040, 1061, 1242, 1377, 1722
BseGI	GGATG	3 cut(s)	1029, 1453, 1621
BseLI	CCNNNNNNNGG	6 cut(s)	234, 257, 260, 502, 1242, 1243
BseMI	GCAATG	3 cut(s)	902, 1378, 1840
BseMII	CTCAG	5 cut(s)	77, 474, 1699, 1961, 2057
BseNI	ACTGG	3 cut(s)	533, 704, 946
BseRI	GAGGAG	9 cut(s)	265, 686, 689, 1199, 1223, 1226, 1762, 2008, 2020
BseSI	GKGCMC	1 cut(s)	1813
BseX3I	CGGCCG	1 cut(s)	973
BseXI	GCAGC	8 cut(s)	45, 67, 597, 816, 830, 875, 1851, 2010
BseYI	CCCAGC	1 cut(s)	8
BsgI	GTGCAG	1 cut(s)	604
Bsh1236I	CGCG	2 cut(s)	1016, 1934
Bsh1285I	CGRYCG	3 cut(s)	94, 976, 1291
BshFI	GGCC	8 cut(s)	68, 239, 272, 899, 975, 1067, 1235, 1678
BshNI	GGYRCC	2 cut(s)	466, 1080
BshTI	ACCGGT	1 cut(s)	1290
BsiEI	CGRYCG	3 cut(s)	94, 976, 1291
BsiHKAI	GWGCWC	3 cut(s)	318, 1686, 1813
BsiHKCI	CYCGRG	4 cut(s)	1039, 1062, 1242, 1814
BslFI	GGGAC	2 cut(s)	1088, 1259
BslI	CCNNNNNNNGG	6 cut(s)	234, 257, 260, 502, 1242, 1243
BsmAI	GTCTC	6 cut(s)	485, 777, 922, 1208, 1272, 1454
BsmBI	CGTCTC	2 cut(s)	1208, 1272
BsmFI	GGGAC	2 cut(s)	1088, 1259
BsmI	GAATGC	2 cut(s)	1113, 1366
BsnI	GGCC	8 cut(s)	68, 239, 272, 899, 975, 1067, 1235, 1678
Bso31I	GGTCTC	3 cut(s)	485, 777, 1454
BsoBI	CYCGRG	4 cut(s)	1039, 1062, 1242, 1814
Bsp119I	TTCGAA	2 cut(s)	720, 1358
Bsp1286I	GDGCHC	3 cut(s)	318, 1686, 1813
Bsp13I	TCCGGA	1 cut(s)	1399
Bsp143I	GATC	6 cut(s)	91, 748, 757, 893, 954, 994
BspANI	GGCC	8 cut(s)	68, 239, 272, 899, 975, 1067, 1235, 1678
BspCNI	CTCAG	5 cut(s)	76, 475, 1698, 1962, 2058
BspEI	TCCGGA	1 cut(s)	1399
BspFNI	CGCG	2 cut(s)	1016, 1934
BspLI	GGNNCC	8 cut(s)	162, 271, 468, 996, 1082, 1188, 1221, 1925
BspMI	ACCTGC	1 cut(s)	2019
BspPI	GGATC	3 cut(s)	901, 989, 1002
BspQI	GCTCTTC	3 cut(s)	99, 1274, 1823
BspT104I	TTCGAA	2 cut(s)	720, 1358
BspT107I	GGYRCC	2 cut(s)	466, 1080
BspTNI	GGTCTC	3 cut(s)	485, 777, 1454
BsrBI	CCGCTC	3 cut(s)	230, 1657, 2010
BsrDI	GCAATG	3 cut(s)	902, 1378, 1840
BsrFI	RCCGGY	3 cut(s)	907, 975, 1290
BsrI	ACTGG	3 cut(s)	533, 704, 946
BssAI	RCCGGY	3 cut(s)	907, 975, 1290
BssECI	CCNNGG	7 cut(s)	546, 998, 1040, 1061, 1242, 1377, 1722
BssMI	GATC	6 cut(s)	91, 748, 757, 893, 954, 994
BssNI	GRCGYC	1 cut(s)	1985
BssSI	CACGAG	1 cut(s)	1069
BssT1I	CCWWGG	3 cut(s)	546, 1377, 1722
Bst2BI	CACGAG	1 cut(s)	1069
Bst2UI	CCWGG	2 cut(s)	434, 1907
Bst4CI	ACNGT	4 cut(s)	51, 933, 1219, 1396
Bst6I	CTCTTC	8 cut(s)	99, 181, 190, 283, 561, 720, 1274, 1823
BstACI	GRCGYC	1 cut(s)	1985
BstBAI	YACGTR	1 cut(s)	1153
BstBI	TTCGAA	2 cut(s)	720, 1358
BstC8I	GCNNGC	2 cut(s)	802, 977
BstDEI	CTNAG	7 cut(s)	63, 233, 483, 1685, 1737, 1970, 2066
BstF5I	GGATG	3 cut(s)	1029, 1453, 1621
BstFNI	CGCG	2 cut(s)	1016, 1934
BstHHI	GCGC	2 cut(s)	14, 1934
BstKTI	GATC	6 cut(s)	94, 751, 760, 896, 957, 997
BstMAI	GTCTC	6 cut(s)	485, 777, 922, 1208, 1272, 1454
BstMBI	GATC	6 cut(s)	91, 748, 757, 893, 954, 994
BstMCI	CGRYCG	3 cut(s)	94, 976, 1291
BstNI	CCWGG	2 cut(s)	434, 1907
BstPAI	GACNNNNGTC	1 cut(s)	1017
BstSCI	CCNGG	7 cut(s)	99, 309, 432, 1222, 1241, 1242, 1905
BstSLI	GKGCMC	1 cut(s)	1813
BstUI	CGCG	2 cut(s)	1016, 1934
BstV1I	GCAGC	8 cut(s)	45, 67, 597, 816, 830, 875, 1851, 2010
BstV2I	GAAGAC	7 cut(s)	504, 529, 561, 567, 603, 654, 1316
BstX2I	RGATCY	2 cut(s)	757, 994
BstYI	RGATCY	2 cut(s)	757, 994
BstZI	CGGCCG	1 cut(s)	973
Bsu36I	CCTNAGG	1 cut(s)	63
BsuRI	GGCC	8 cut(s)	68, 239, 272, 899, 975, 1067, 1235, 1678
BtsCI	GGATG	3 cut(s)	1029, 1453, 1621
BtsI	GCAGTG	1 cut(s)	1639
BtsIMutI	CAGTG	2 cut(s)	587, 1639
BveI	ACCTGC	1 cut(s)	2019
Cac8I	GCNNGC	2 cut(s)	802, 977
CaiI	CAGNNNCTG	2 cut(s)	810, 868
CfoI	GCGC	2 cut(s)	14, 1934
Cfr10I	RCCGGY	3 cut(s)	907, 975, 1290
Cfr13I	GGNCC	9 cut(s)	67, 237, 270, 530, 1065, 1147, 1234, 1293, 2031
Cfr9I	CCCGGG	1 cut(s)	1242
CseI	GACGC	5 cut(s)	488, 1022, 1145, 1175, 1993
CsiI	ACCWGGT	1 cut(s)	1905
Csp6I	GTAC	4 cut(s)	1045, 1081, 2063, 2074
CspAI	ACCGGT	1 cut(s)	1290
CviAII	CATG	6 cut(s)	132, 742, 1195, 1784, 1822, 2039
CviQI	GTAC	4 cut(s)	1045, 1081, 2063, 2074
DdeI	CTNAG	7 cut(s)	63, 233, 483, 1685, 1737, 1970, 2066
DpnI	GATC	6 cut(s)	93, 750, 759, 895, 956, 996
DpnII	GATC	6 cut(s)	91, 748, 757, 893, 954, 994
DriI	GACNNNNNGTC	1 cut(s)	1264
EaeI	YGGCCR	3 cut(s)	897, 973, 1676
EagI	CGGCCG	1 cut(s)	973
Eam1104I	CTCTTC	8 cut(s)	99, 181, 190, 283, 561, 720, 1274, 1823
Eam1105I	GACNNNNNGTC	1 cut(s)	1264
EarI	CTCTTC	8 cut(s)	99, 181, 190, 283, 561, 720, 1274, 1823
EciI	GGCGGA	3 cut(s)	895, 1899, 2028
Ecl136II	GAGCTC	2 cut(s)	316, 1684
EclXI	CGGCCG	1 cut(s)	973
Eco130I	CCWWGG	3 cut(s)	546, 1377, 1722
Eco24I	GRGCYC	2 cut(s)	318, 1686
Eco31I	GGTCTC	3 cut(s)	485, 777, 1454
Eco47I	GGWCC	4 cut(s)	530, 1147, 1293, 2031
Eco52I	CGGCCG	1 cut(s)	973
Eco53kI	GAGCTC	2 cut(s)	316, 1684
Eco57I	CTGAAG	3 cut(s)	807, 809, 890
Eco72I	CACGTG	1 cut(s)	1153
Eco81I	CCTNAGG	1 cut(s)	63
Eco88I	CYCGRG	4 cut(s)	1039, 1062, 1242, 1814
EcoICRI	GAGCTC	2 cut(s)	316, 1684
EcoO109I	RGGNCCY	1 cut(s)	270
EcoRI	GAATTC	2 cut(s)	1198, 1435
EcoRII	CCWGG	2 cut(s)	432, 1905
EcoT14I	CCWWGG	3 cut(s)	546, 1377, 1722
EcoT38I	GRGCYC	2 cut(s)	318, 1686
ErhI	CCWWGG	3 cut(s)	546, 1377, 1722
Esp3I	CGTCTC	2 cut(s)	1208, 1272
FaeI	CATG	6 cut(s)	135, 745, 1198, 1787, 1825, 2042
FaqI	GGGAC	2 cut(s)	1088, 1259
FatI	CATG	6 cut(s)	131, 741, 1194, 1783, 1821, 2038
FauI	CCCGC	3 cut(s)	793, 1009, 1220
FokI	GGATG	3 cut(s)	1036, 1460, 1628
FriOI	GRGCYC	2 cut(s)	318, 1686
FspBI	CTAG	2 cut(s)	1548, 1764
GlaI	GCGC	2 cut(s)	13, 1933
GsaI	CCCAGC	1 cut(s)	12
GsuI	CTGGAG	1 cut(s)	2023
HaeIII	GGCC	8 cut(s)	68, 239, 272, 899, 975, 1067, 1235, 1678
HgaI	GACGC	5 cut(s)	488, 1022, 1145, 1175, 1993
HhaI	GCGC	2 cut(s)	14, 1934
Hin1I	GRCGYC	1 cut(s)	1985
Hin1II	CATG	6 cut(s)	135, 745, 1198, 1787, 1825, 2042
Hin6I	GCGC	2 cut(s)	12, 1932
HinP1I	GCGC	2 cut(s)	12, 1932
HindIII	AAGCTT	1 cut(s)	1739
HinfI	GANTC	8 cut(s)	4, 448, 542, 764, 1259, 1496, 1670, 2016
HphI	GGTGA	1 cut(s)	422
Hpy166II	GTNNAC	2 cut(s)	1150, 1811
Hpy188III	TCNNGA	7 cut(s)	195, 659, 761, 925, 1400, 1816, 2020
Hpy8I	GTNNAC	2 cut(s)	1150, 1811
Hpy99I	CGWCG	7 cut(s)	55, 398, 443, 1016, 1037, 1169, 1267
HpyAV	CCTTC	5 cut(s)	174, 454, 784, 1086, 1881
HpyCH4III	ACNGT	4 cut(s)	51, 933, 1219, 1396
HpyCH4IV	ACGT	3 cut(s)	396, 1097, 1152
HpyCH4V	TGCA	3 cut(s)	26, 585, 1811
HpyF3I	CTNAG	7 cut(s)	63, 233, 483, 1685, 1737, 1970, 2066
HpySE526I	ACGT	3 cut(s)	396, 1097, 1152
Hsp92I	GRCGYC	1 cut(s)	1985
Hsp92II	CATG	6 cut(s)	135, 745, 1198, 1787, 1825, 2042
HspAI	GCGC	2 cut(s)	12, 1932
Kpn2I	TCCGGA	1 cut(s)	1399
KpnI	GGTACC	1 cut(s)	1084
KroI	GCCGGC	1 cut(s)	975
KroNI	GCCGGC	1 cut(s)	977
Kzo9I	GATC	6 cut(s)	91, 748, 757, 893, 954, 994
LguI	GCTCTTC	3 cut(s)	99, 1274, 1823
Lsp1109I	GCAGC	8 cut(s)	45, 67, 597, 816, 830, 875, 1851, 2010
LweI	GCATC	2 cut(s)	1107, 1151
MabI	ACCWGGT	1 cut(s)	1905
MaeI	CTAG	2 cut(s)	1548, 1764
MaeII	ACGT	3 cut(s)	396, 1097, 1152
MaeIII	GTNAC	2 cut(s)	640, 706
MalI	GATC	6 cut(s)	93, 750, 759, 895, 956, 996
MbiI	CCGCTC	3 cut(s)	230, 1657, 2010
MboI	GATC	6 cut(s)	91, 748, 757, 893, 954, 994
MfeI	CAATTG	1 cut(s)	627
MflI	RGATCY	2 cut(s)	757, 994
MhlI	GDGCHC	3 cut(s)	318, 1686, 1813
MlyI	GAGTC	4 cut(s)	551, 1253, 1679, 2025
MmeI	TCCRAC	6 cut(s)	138, 240, 930, 1001, 1320, 1748
MroI	TCCGGA	1 cut(s)	1399
MroNI	GCCGGC	1 cut(s)	975
MroXI	GAANNNNTTC	1 cut(s)	687
MseI	TTAA	1 cut(s)	1731
MslI	CAYNNNNRTG	1 cut(s)	1788
MspA1I	CMGCKG	3 cut(s)	58, 588, 2001
MspR9I	CCNGG	7 cut(s)	101, 311, 434, 1224, 1243, 1244, 1907
MunI	CAATTG	1 cut(s)	627
Mva1269I	GAATGC	2 cut(s)	1113, 1366
MvaI	CCWGG	2 cut(s)	434, 1907
MvnI	CGCG	2 cut(s)	1016, 1934
NaeI	GCCGGC	1 cut(s)	977
NciI	CCSGG	5 cut(s)	101, 311, 1224, 1243, 1244
NdeII	GATC	6 cut(s)	91, 748, 757, 893, 954, 994
NgoMIV	GCCGGC	1 cut(s)	975
NlaIII	CATG	6 cut(s)	135, 745, 1198, 1787, 1825, 2042
NlaIV	GGNNCC	8 cut(s)	162, 271, 468, 996, 1082, 1188, 1221, 1925
NmuCI	GTSAC	2 cut(s)	640, 706
NspV	TTCGAA	2 cut(s)	720, 1358
PciSI	GCTCTTC	3 cut(s)	99, 1274, 1823
PcsI	WCGNNNNNNNCGW	1 cut(s)	570
PctI	GAATGC	2 cut(s)	1113, 1366
PdiI	GCCGGC	1 cut(s)	977
PdmI	GAANNNNTTC	1 cut(s)	687
PfeI	GAWTC	4 cut(s)	4, 448, 764, 1496
PfoI	TCCNGGA	1 cut(s)	309
PinAI	ACCGGT	1 cut(s)	1290
Ple19I	CGATCG	1 cut(s)	94
PleI	GAGTC	4 cut(s)	550, 1253, 1678, 2024
PmaCI	CACGTG	1 cut(s)	1153
PmlI	CACGTG	1 cut(s)	1153
PpsI	GAGTC	4 cut(s)	550, 1253, 1678, 2024
Ppu21I	YACGTR	1 cut(s)	1153
PshAI	GACNNNNGTC	1 cut(s)	1017
Psp124BI	GAGCTC	2 cut(s)	318, 1686
Psp6I	CCWGG	2 cut(s)	432, 1905
PspCI	CACGTG	1 cut(s)	1153
PspFI	CCCAGC	1 cut(s)	8
PspGI	CCWGG	2 cut(s)	432, 1905
PspN4I	GGNNCC	8 cut(s)	162, 271, 468, 996, 1082, 1188, 1221, 1925
PspPI	GGNCC	9 cut(s)	67, 237, 270, 530, 1065, 1147, 1234, 1293, 2031
PstNI	CAGNNNCTG	2 cut(s)	810, 868
PsuI	RGATCY	2 cut(s)	757, 994
PvuI	CGATCG	1 cut(s)	94
PvuII	CAGCTG	2 cut(s)	58, 2001
RgaI	GCGATCGC	1 cut(s)	94
RsaI	GTAC	4 cut(s)	1046, 1082, 2064, 2075
RsaNI	GTAC	4 cut(s)	1045, 1081, 2063, 2074
RseI	CAYNNNNRTG	1 cut(s)	1788
SacI	GAGCTC	2 cut(s)	318, 1686
SapI	GCTCTTC	3 cut(s)	99, 1274, 1823
SaqAI	TTAA	1 cut(s)	1731
Sau3AI	GATC	6 cut(s)	91, 748, 757, 893, 954, 994
Sau96I	GGNCC	9 cut(s)	67, 237, 270, 530, 1065, 1147, 1234, 1293, 2031
SchI	GAGTC	4 cut(s)	551, 1253, 1679, 2025
ScrFI	CCNGG	7 cut(s)	101, 311, 434, 1224, 1243, 1244, 1907
SduI	GDGCHC	3 cut(s)	318, 1686, 1813
SexAI	ACCWGGT	1 cut(s)	1905
SfaAI	GCGATCGC	1 cut(s)	94
SfaNI	GCATC	2 cut(s)	1107, 1151
SfuI	TTCGAA	2 cut(s)	720, 1358
SgfI	GCGATCGC	1 cut(s)	94
SinI	GGWCC	4 cut(s)	530, 1147, 1293, 2031
SmaI	CCCGGG	1 cut(s)	1244
SmiMI	CAYNNNNRTG	1 cut(s)	1788
SspMI	CTAG	2 cut(s)	1548, 1764
SstI	GAGCTC	2 cut(s)	318, 1686
StyD4I	CCNGG	7 cut(s)	99, 309, 432, 1222, 1241, 1242, 1905
StyI	CCWWGG	3 cut(s)	546, 1377, 1722
TaaI	ACNGT	4 cut(s)	51, 933, 1219, 1396
TaiI	ACGT	3 cut(s)	399, 1100, 1155
TatI	WGTACW	1 cut(s)	2073
TfiI	GAWTC	4 cut(s)	4, 448, 764, 1496
Tru1I	TTAA	1 cut(s)	1731
Tru9I	TTAA	1 cut(s)	1731
TscAI	CASTG	2 cut(s)	587, 1639
TseFI	GTSAC	2 cut(s)	640, 706
TseI	GCWGC	8 cut(s)	55, 58, 585, 804, 818, 888, 1839, 1998
Tsp45I	GTSAC	2 cut(s)	640, 706
TspDTI	ATGAA	4 cut(s)	17, 758, 1211, 1361
TspGWI	ACGGA	6 cut(s)	456, 460, 725, 1007, 1936, 1977
TspMI	CCCGGG	1 cut(s)	1242
TspRI	CASTG	2 cut(s)	587, 1639
VneI	GTGCAC	1 cut(s)	1809
VpaK11BI	GGWCC	4 cut(s)	530, 1147, 1293, 2031
XapI	RAATTY	7 cut(s)	499, 612, 686, 1173, 1198, 1421, 1435
XcmI	CCANNNNNNNNNTGG	1 cut(s)	907
XmaI	CCCGGG	1 cut(s)	1242
XmnI	GAANNNNTTC	1 cut(s)	687
XspI	CTAG	2 cut(s)	1548, 1764

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.