Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0013274

Physical Location & Seq

Genomic Coordinates Reverse (-)

24017802 .. 24019901

Gene Structure 2100 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1160400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2100 bp

ATGAATCCCAGCGCAGAAGCAGCTGCACCGCCGCCGCCACCGCTTCAACCGTCGCAGCTGCCTCAGGCCCACAGACTTTCCACCTCCTGCGACCGCCACCCGGAAGAGCATTTCACGGGCTTCTGCCCCTCATGCCTCTGCGAACGCCTTGCCGTGTTGGAGCCTTCAACAAACTCTTCTTCCTCTTCCACTTCTCGAAAACCCCCTACTTCCTCCACCGCCGCCGCCGCTCTTAGGGCCATTTTCAAGCCCCCCATTGGCGGTGGTGGGGCCTCATCTTCCAATAAAAACCGACCCACCTCATTTTTCCCGGAGCTCCGACGAACCAAGTCTTTCTCTGCCTCTAAAAACGAGGGTTTCTCCGGCGTGTTTGAGCCGCAGAGGAAGTCTTGCGACGTTAGGGTTCGGAACACTCTCTGGACACTCTTTCACCAGGACGACGGACGGAATCCTAACAAGAAGGAAGCACCACCTACTGACGCTGAGGTTGAGACCCGAAATTTGGGTGGGTCTTCTTCCAGCTTTCGAGGTCCAGTCTTCGAGTCCAAGGAGGAAGACGAAGACGAAGAGGACGAAAATCAGTGCAGCGGCGGCGAAGACTTTGGAATTGAAATTTGCGAGGAAACAATTGTCGCCAATGTGACAGAAGACAAAGTTCCAGAAATCGTAGAGGAGGAGGAAGAAGAATTTCAACCACCGCAACCAGTCCCGGAACAATTCGAAGAAGAGGGCTTGAAGCCCATGAAAGATCATATAGATCTTGATTCACAGACGAAGAAGCGAGACCTGAAGGAGATTGCGGGCAGCTTCTGGTCAGCAGCTTCAGTTTTCAGCAAAAAATTACAGAAATGGAGGCAGAAGCAGAAGCTGAAGAAGCGGCGGAACGGCTGCGGATCGGCCACATTGCCGGTGGAGAAGCCAATCGGGAGACAGTTCAGAGAAACCCAGTCGGAGATCGCCGATTACGGCTTCGGCCGGCGTTCCTGCGACACGGATCCGAGGTTTTCGCTTGACGCGGGTCGGATGTCGCTCGACGCGGGTCGGATGTCGTTCGACGACCCGAGATACTCCTTTGACGAGCCTCGGGCCTCGTGGGACGGGTACCTCATCGGAAGGACGTTTCCGAGGATGCCCACGATGCTCTCGGTGGTGGAGGACGCTCCGGTGATCCACGTGGCGAGATGCGACGCTCAAATTCCGGTTGAGGAGCCGCCCATGAATTCCATTGAGGAAGAGACGGTTCCGGGCGGGTCGGCCCAGACCCGGGACTATTATTTGGACTCGTCGTCTCGCCGGAGGAAGAGCCTCGACCGGTCCAACTCCATTAGGAAGACTGCGGAGGCGGTGGTGGCGGATATTGATGAGAGGAAGTCGGTTTCGAATGCCAATGCCAATGCCAAGGTGTCGCCCACGACTGTTCCGGATAACAGAGATTTGAGGGAATTTTACAACTCGAATTCTTTGAGGGATGACTGCTCGGAGACCTTTGAAACGGGGTCGTTTCGGGGCGGCGGTGATTCGGCATCGGTGGTGGGAAATGGGGAGAGGAATAAAGGGTGTAAAAAGTCTTGGAGGTGGAGCAAGGCGTGGAATATATGGAGTTTTATACACAGGAGGGGTGGGAACAAAGATGAGGATGACGATAGGTATAGCAGTGTTAGAGCAAACGGGGTGGAGCGGTCTTTTTCGGAGTCGTGGCCGGAGCTCAGAGGCAGCGGTGGTGGCGAGAGGAATGGGGAAGCCAAGGGCTTTAATCCTAAGCTTATGAGGAGTAATAGTAGTGCTAGTTGGAGGAATGGTTCTCATGGGTATGGCGGTTTTGGTGGCGGTGTATTCGGGAGCATGAGGAAGAGCAATGGCAGCGTTCCTGTGGTCGAGGCAAACGGGTTTGGAAATGGGAACGGCGGAAGGAAGAAGAAAGACGATCAGGTTGTGCTGGAACGGAACCGGAGCGCAAGGTATTCCCCGAACCATATTGACAACGGATTGCTGAGGTTTTACTTGACGCCGATGAGGAGCAGCTGGAGGAGCGGCGGAGTCGGGAAAAGCAGGTCCAGCCATGCTCATTCGATTGCCAGAAGCGTACTGAGATTGTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009965 GO:0010016 GO:0010051 GO:0010087 GO:0010088 GO:0010154 GO:0010232 GO:0010233 GO:0010588 GO:0016020 GO:0016324 GO:0022414 GO:0022622 GO:0032501 GO:0032502 GO:0044425 GO:0044459 GO:0044464 GO:0045177 GO:0048316 GO:0048366 GO:0048367 GO:0048598 GO:0048608 GO:0048731 GO:0048825 GO:0048826 GO:0048827 GO:0048856 GO:0051179 GO:0051234 GO:0061458 GO:0071944 GO:0090698 GO:0098590 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF740

Protein Analysis

700

Amino Acids

77.41

Weight (kDa)

7.54

Isoelectric Point (pI)

64.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF740	PF05340	17 - 675	9.8e-259	Protein of unknown function (DUF740)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013274)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38070
fragaria_vesca	FvH4_6g12000
malus_domestica	MD04G1147100.v1.1 MD12G1160400.v1.1
prunus_persica	Prupe.6G270900_v2.0.a1
pyrus_communis	pycom04g13290 pycom12g15220
rosa_chinensis	RchiOBHm_Chr3g0463831
rosa_laevigata	RLG00000024753
rosa_multiflora	Rmu_sc0004796.1_g000009
rosa_roxburghii	Rroxscaffold_6G00399380 Rroxscaffold_6G00416260
rosa_rugosa	Rorug03G0068100
rosa_samantha	Rh3AG125200 Rh3BG129500 Rh3CG132000 Rh3DG130500
rosa_wichuraiana	Rw3G010580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2040
Acc65I	GGTACC	1 cut(s)	1101
AccB1I	GGYRCC	1 cut(s)	1101
AccBSI	CCGCTC	3 cut(s)	230, 1678, 2031
AccII	CGCG	2 cut(s)	1016, 1037
AccIII	TCCGGA	1 cut(s)	1420
AclWI	GGATC	4 cut(s)	901, 989, 1002, 1162
AcoI	YGGCCR	3 cut(s)	897, 973, 1697
AcsI	RAATTY	7 cut(s)	499, 612, 686, 1194, 1219, 1442, 1456
AcuI	CTGAAG	3 cut(s)	807, 809, 890
AcvI	CACGTG	1 cut(s)	1174
AcyI	GRCGYC	1 cut(s)	2006
AfaI	GTAC	3 cut(s)	1103, 2085, 2096
AfiI	CCNNNNNNNGG	7 cut(s)	100, 234, 257, 260, 502, 1263, 1264
AgeI	ACCGGT	1 cut(s)	1311
AgsI	TTSAA	7 cut(s)	47, 168, 247, 611, 692, 736, 1490
AhdI	GACNNNNNGTC	1 cut(s)	1285
AjnI	CCWGG	1 cut(s)	432
AloI	GAACNNNNNNTCC	2 cut(s)	1783, 1815
Alw21I	GWGCWC	2 cut(s)	318, 1707
Alw26I	GTCTC	6 cut(s)	485, 777, 922, 1229, 1293, 1475
AlwI	GGATC	4 cut(s)	901, 989, 1002, 1162
AlwNI	CAGNNNCTG	2 cut(s)	810, 868
Ama87I	CYCGRG	3 cut(s)	1060, 1083, 1263
Aor13HI	TCCGGA	1 cut(s)	1420
AoxI	GGCC	8 cut(s)	66, 237, 270, 897, 973, 1086, 1254, 1697
ApoI	RAATTY	7 cut(s)	499, 612, 686, 1194, 1219, 1442, 1456
ArsI	GACNNNNNNTTYG	2 cut(s)	484, 516
AsiGI	ACCGGT	1 cut(s)	1311
Asp700I	GAANNNNTTC	1 cut(s)	687
Asp718I	GGTACC	1 cut(s)	1101
AspLEI	GCGC	2 cut(s)	14, 1955
AspS9I	GGNCC	8 cut(s)	67, 237, 270, 530, 1086, 1255, 1314, 2052
AsuC2I	CCSGG	6 cut(s)	101, 311, 710, 1245, 1264, 1265
AsuHPI	GGTGA	3 cut(s)	422, 1177, 1526
AsuII	TTCGAA	2 cut(s)	720, 1379
AvaI	CYCGRG	3 cut(s)	1060, 1083, 1263
AvaII	GGWCC	3 cut(s)	530, 1314, 2052
AxyI	CCTNAGG	1 cut(s)	63
BamHI	GGATCC	1 cut(s)	994
BanI	GGYRCC	1 cut(s)	1101
BanII	GRGCYC	2 cut(s)	318, 1707
BauI	CACGAG	1 cut(s)	1090
BbrPI	CACGTG	1 cut(s)	1174
BbsI	GAAGAC	7 cut(s)	504, 529, 561, 567, 603, 654, 1337
Bbv12I	GWGCWC	2 cut(s)	318, 1707
BbvCI	CCTCAGC	2 cut(s)	483, 1991
BceAI	ACGGC	4 cut(s)	137, 901, 982, 1918
BcgI	CGANNNNNNTGC	2 cut(s)	131, 165
BciT130I	CCWGG	1 cut(s)	434
BcnI	CCSGG	6 cut(s)	101, 311, 710, 1245, 1264, 1265
BcoDI	GTCTC	6 cut(s)	485, 777, 922, 1229, 1293, 1475
BfaI	CTAG	1 cut(s)	1785
BfuAI	ACCTGC	1 cut(s)	2040
BglII	AGATCT	1 cut(s)	757
Bme1390I	CCNGG	7 cut(s)	101, 311, 434, 710, 1245, 1264, 1265
Bme18I	GGWCC	3 cut(s)	530, 1314, 2052
BmeRI	GACNNNNNGTC	1 cut(s)	1285
BmeT110I	CYCGRG	3 cut(s)	1060, 1083, 1263
BmgT120I	GGNCC	8 cut(s)	67, 237, 270, 530, 1086, 1255, 1314, 2052
BmiI	GGNNCC	7 cut(s)	162, 271, 996, 1103, 1209, 1242, 1946
BmrFI	CCNGG	7 cut(s)	101, 311, 434, 710, 1245, 1264, 1265
BmrI	ACTGGG	1 cut(s)	940
BmsI	GCATC	4 cut(s)	1119, 1128, 1172, 1532
BmuI	ACTGGG	1 cut(s)	940
BoxI	GACNNNNGTC	2 cut(s)	1017, 1038
BpiI	GAAGAC	7 cut(s)	504, 529, 561, 567, 603, 654, 1337
BplI	GAGNNNNNCTC	2 cut(s)	344, 376
BpmI	CTGGAG	1 cut(s)	2044
Bpu10I	CCTNAGC	3 cut(s)	483, 1758, 1991
Bpu14I	TTCGAA	2 cut(s)	720, 1379
BpuMI	CCSGG	6 cut(s)	101, 311, 710, 1245, 1264, 1265
BsaAI	YACGTR	1 cut(s)	1174
BsaHI	GRCGYC	1 cut(s)	2006
BsaI	GGTCTC	3 cut(s)	485, 777, 1475
BsaJI	CCNNGG	7 cut(s)	546, 998, 1082, 1124, 1263, 1398, 1743
BsaWI	WCCGGW	5 cut(s)	1162, 1198, 1311, 1420, 1947
BsaXI	ACNNNNNCTCC	4 cut(s)	1762, 1783, 1792, 1813
Bsc4I	CCNNNNNNNGG	7 cut(s)	100, 234, 257, 260, 502, 1263, 1264
Bse118I	RCCGGY	3 cut(s)	907, 975, 1311
Bse1I	ACTGG	3 cut(s)	533, 704, 946
Bse21I	CCTNAGG	1 cut(s)	63
Bse3DI	GCAATG	2 cut(s)	902, 1861
BseAI	TCCGGA	1 cut(s)	1420
BseBI	CCWGG	1 cut(s)	434
BseDI	CCNNGG	7 cut(s)	546, 998, 1082, 1124, 1263, 1398, 1743
BseGI	GGATG	5 cut(s)	1029, 1050, 1134, 1474, 1642
BseLI	CCNNNNNNNGG	7 cut(s)	100, 234, 257, 260, 502, 1263, 1264
BseMI	GCAATG	2 cut(s)	902, 1861
BseMII	CTCAG	5 cut(s)	77, 474, 1720, 1982, 2078
BseNI	ACTGG	3 cut(s)	533, 704, 946
BseRI	GAGGAG	6 cut(s)	686, 689, 1220, 1783, 2029, 2041
BseX3I	CGGCCG	1 cut(s)	973
BseYI	CCCAGC	1 cut(s)	8
BsgI	GTGCAG	2 cut(s)	9, 604
Bsh1236I	CGCG	2 cut(s)	1016, 1037
Bsh1285I	CGRYCG	3 cut(s)	94, 976, 1312
BshFI	GGCC	8 cut(s)	68, 239, 272, 899, 975, 1088, 1256, 1699
BshNI	GGYRCC	1 cut(s)	1101
BshTI	ACCGGT	1 cut(s)	1311
BsiEI	CGRYCG	3 cut(s)	94, 976, 1312
BsiHKAI	GWGCWC	2 cut(s)	318, 1707
BsiHKCI	CYCGRG	3 cut(s)	1060, 1083, 1263
BslFI	GGGAC	3 cut(s)	692, 1109, 1280
BslI	CCNNNNNNNGG	7 cut(s)	100, 234, 257, 260, 502, 1263, 1264
BsmAI	GTCTC	6 cut(s)	485, 777, 922, 1229, 1293, 1475
BsmBI	CGTCTC	2 cut(s)	1229, 1293
BsmFI	GGGAC	3 cut(s)	692, 1109, 1280
BsmI	GAATGC	1 cut(s)	1387
BsnI	GGCC	8 cut(s)	68, 239, 272, 899, 975, 1088, 1256, 1699
Bso31I	GGTCTC	3 cut(s)	485, 777, 1475
BsoBI	CYCGRG	3 cut(s)	1060, 1083, 1263
Bsp119I	TTCGAA	2 cut(s)	720, 1379
Bsp1286I	GDGCHC	2 cut(s)	318, 1707
Bsp13I	TCCGGA	1 cut(s)	1420
Bsp143I	GATC	7 cut(s)	748, 757, 893, 954, 994, 1167, 1924
BspANI	GGCC	8 cut(s)	68, 239, 272, 899, 975, 1088, 1256, 1699
BspCNI	CTCAG	5 cut(s)	76, 475, 1719, 1983, 2079
BspEI	TCCGGA	1 cut(s)	1420
BspFNI	CGCG	2 cut(s)	1016, 1037
BspLI	GGNNCC	7 cut(s)	162, 271, 996, 1103, 1209, 1242, 1946
BspMI	ACCTGC	1 cut(s)	2040
BspPI	GGATC	4 cut(s)	901, 989, 1002, 1162
BspQI	GCTCTTC	3 cut(s)	99, 1295, 1844
BspT104I	TTCGAA	2 cut(s)	720, 1379
BspT107I	GGYRCC	1 cut(s)	1101
BspTNI	GGTCTC	3 cut(s)	485, 777, 1475
BsrBI	CCGCTC	3 cut(s)	230, 1678, 2031
BsrDI	GCAATG	2 cut(s)	902, 1861
BsrFI	RCCGGY	3 cut(s)	907, 975, 1311
BsrI	ACTGG	3 cut(s)	533, 704, 946
BssAI	RCCGGY	3 cut(s)	907, 975, 1311
BssECI	CCNNGG	7 cut(s)	546, 998, 1082, 1124, 1263, 1398, 1743
BssMI	GATC	7 cut(s)	748, 757, 893, 954, 994, 1167, 1924
BssNI	GRCGYC	1 cut(s)	2006
BssSI	CACGAG	1 cut(s)	1090
BssT1I	CCWWGG	3 cut(s)	546, 1398, 1743
Bst2BI	CACGAG	1 cut(s)	1090
Bst2UI	CCWGG	1 cut(s)	434
Bst4CI	ACNGT	4 cut(s)	51, 933, 1240, 1417
Bst6I	CTCTTC	8 cut(s)	99, 181, 190, 561, 720, 1227, 1295, 1844
BstACI	GRCGYC	1 cut(s)	2006
BstBAI	YACGTR	1 cut(s)	1174
BstBI	TTCGAA	2 cut(s)	720, 1379
BstC8I	GCNNGC	2 cut(s)	802, 977
BstDEI	CTNAG	7 cut(s)	63, 233, 483, 1706, 1758, 1991, 2087
BstF5I	GGATG	5 cut(s)	1029, 1050, 1134, 1474, 1642
BstFNI	CGCG	2 cut(s)	1016, 1037
BstHHI	GCGC	2 cut(s)	14, 1955
BstKTI	GATC	7 cut(s)	751, 760, 896, 957, 997, 1170, 1927
BstMAI	GTCTC	6 cut(s)	485, 777, 922, 1229, 1293, 1475
BstMBI	GATC	7 cut(s)	748, 757, 893, 954, 994, 1167, 1924
BstMCI	CGRYCG	3 cut(s)	94, 976, 1312
BstNI	CCWGG	1 cut(s)	434
BstPAI	GACNNNNGTC	2 cut(s)	1017, 1038
BstSCI	CCNGG	7 cut(s)	99, 309, 432, 708, 1243, 1262, 1263
BstUI	CGCG	2 cut(s)	1016, 1037
BstV2I	GAAGAC	7 cut(s)	504, 529, 561, 567, 603, 654, 1337
BstX2I	RGATCY	2 cut(s)	757, 994
BstYI	RGATCY	2 cut(s)	757, 994
BstZI	CGGCCG	1 cut(s)	973
Bsu36I	CCTNAGG	1 cut(s)	63
BsuRI	GGCC	8 cut(s)	68, 239, 272, 899, 975, 1088, 1256, 1699
BtsCI	GGATG	5 cut(s)	1029, 1050, 1134, 1474, 1642
BtsI	GCAGTG	1 cut(s)	1660
BtsIMutI	CAGTG	2 cut(s)	587, 1660
BveI	ACCTGC	1 cut(s)	2040
Cac8I	GCNNGC	2 cut(s)	802, 977
CaiI	CAGNNNCTG	2 cut(s)	810, 868
CfoI	GCGC	2 cut(s)	14, 1955
Cfr10I	RCCGGY	3 cut(s)	907, 975, 1311
Cfr13I	GGNCC	8 cut(s)	67, 237, 270, 530, 1086, 1255, 1314, 2052
Cfr9I	CCCGGG	1 cut(s)	1263
CseI	GACGC	6 cut(s)	488, 1022, 1043, 1166, 1196, 2014
Csp6I	GTAC	3 cut(s)	1102, 2084, 2095
CspAI	ACCGGT	1 cut(s)	1311
CspCI	CAANNNNNGTGG	2 cut(s)	1653, 1688
CviAII	CATG	6 cut(s)	132, 742, 1216, 1805, 1843, 2060
CviQI	GTAC	3 cut(s)	1102, 2084, 2095
DdeI	CTNAG	7 cut(s)	63, 233, 483, 1706, 1758, 1991, 2087
DpnI	GATC	7 cut(s)	750, 759, 895, 956, 996, 1169, 1926
DpnII	GATC	7 cut(s)	748, 757, 893, 954, 994, 1167, 1924
DriI	GACNNNNNGTC	1 cut(s)	1285
EaeI	YGGCCR	3 cut(s)	897, 973, 1697
EagI	CGGCCG	1 cut(s)	973
Eam1104I	CTCTTC	8 cut(s)	99, 181, 190, 561, 720, 1227, 1295, 1844
Eam1105I	GACNNNNNGTC	1 cut(s)	1285
EarI	CTCTTC	8 cut(s)	99, 181, 190, 561, 720, 1227, 1295, 1844
EciI	GGCGGA	4 cut(s)	895, 1367, 1920, 2049
Ecl136II	GAGCTC	2 cut(s)	316, 1705
EclXI	CGGCCG	1 cut(s)	973
Eco130I	CCWWGG	3 cut(s)	546, 1398, 1743
Eco24I	GRGCYC	2 cut(s)	318, 1707
Eco31I	GGTCTC	3 cut(s)	485, 777, 1475
Eco47I	GGWCC	3 cut(s)	530, 1314, 2052
Eco52I	CGGCCG	1 cut(s)	973
Eco53kI	GAGCTC	2 cut(s)	316, 1705
Eco57I	CTGAAG	3 cut(s)	807, 809, 890
Eco72I	CACGTG	1 cut(s)	1174
Eco81I	CCTNAGG	1 cut(s)	63
Eco88I	CYCGRG	3 cut(s)	1060, 1083, 1263
EcoICRI	GAGCTC	2 cut(s)	316, 1705
EcoO109I	RGGNCCY	1 cut(s)	270
EcoRI	GAATTC	2 cut(s)	1219, 1456
EcoRII	CCWGG	1 cut(s)	432
EcoT14I	CCWWGG	3 cut(s)	546, 1398, 1743
EcoT38I	GRGCYC	2 cut(s)	318, 1707
ErhI	CCWWGG	3 cut(s)	546, 1398, 1743
Esp3I	CGTCTC	2 cut(s)	1229, 1293
FaeI	CATG	6 cut(s)	135, 745, 1219, 1808, 1846, 2063
FaqI	GGGAC	3 cut(s)	692, 1109, 1280
FatI	CATG	6 cut(s)	131, 741, 1215, 1804, 1842, 2059
FauI	CCCGC	4 cut(s)	793, 1009, 1030, 1241
FokI	GGATG	5 cut(s)	1036, 1057, 1141, 1481, 1649
FriOI	GRGCYC	2 cut(s)	318, 1707
FspBI	CTAG	1 cut(s)	1785
GlaI	GCGC	2 cut(s)	13, 1954
GsaI	CCCAGC	1 cut(s)	12
GsuI	CTGGAG	1 cut(s)	2044
HaeIII	GGCC	8 cut(s)	68, 239, 272, 899, 975, 1088, 1256, 1699
HgaI	GACGC	6 cut(s)	488, 1022, 1043, 1166, 1196, 2014
HhaI	GCGC	2 cut(s)	14, 1955
Hin1I	GRCGYC	1 cut(s)	2006
Hin1II	CATG	6 cut(s)	135, 745, 1219, 1808, 1846, 2063
Hin6I	GCGC	2 cut(s)	12, 1953
HinP1I	GCGC	2 cut(s)	12, 1953
HindIII	AAGCTT	1 cut(s)	1760
HinfI	GANTC	8 cut(s)	4, 448, 542, 764, 1280, 1517, 1691, 2037
HphI	GGTGA	3 cut(s)	422, 1177, 1526
Hpy188III	TCNNGA	8 cut(s)	195, 418, 659, 761, 925, 1421, 1837, 2041
Hpy99I	CGWCG	8 cut(s)	55, 324, 398, 443, 1037, 1058, 1190, 1288
HpyAV	CCTTC	5 cut(s)	174, 454, 784, 1107, 1902
HpyCH4III	ACNGT	4 cut(s)	51, 933, 1240, 1417
HpyCH4IV	ACGT	3 cut(s)	396, 1118, 1173
HpyCH4V	TGCA	2 cut(s)	26, 585
HpyF3I	CTNAG	7 cut(s)	63, 233, 483, 1706, 1758, 1991, 2087
HpySE526I	ACGT	3 cut(s)	396, 1118, 1173
Hsp92I	GRCGYC	1 cut(s)	2006
Hsp92II	CATG	6 cut(s)	135, 745, 1219, 1808, 1846, 2063
HspAI	GCGC	2 cut(s)	12, 1953
Kpn2I	TCCGGA	1 cut(s)	1420
KpnI	GGTACC	1 cut(s)	1105
KroI	GCCGGC	1 cut(s)	975
KroNI	GCCGGC	1 cut(s)	977
Kzo9I	GATC	7 cut(s)	748, 757, 893, 954, 994, 1167, 1924
LguI	GCTCTTC	3 cut(s)	99, 1295, 1844
LweI	GCATC	4 cut(s)	1119, 1128, 1172, 1532
MaeI	CTAG	1 cut(s)	1785
MaeII	ACGT	3 cut(s)	396, 1118, 1173
MaeIII	GTNAC	1 cut(s)	640
MalI	GATC	7 cut(s)	750, 759, 895, 956, 996, 1169, 1926
MbiI	CCGCTC	3 cut(s)	230, 1678, 2031
MboI	GATC	7 cut(s)	748, 757, 893, 954, 994, 1167, 1924
MfeI	CAATTG	1 cut(s)	627
MflI	RGATCY	2 cut(s)	757, 994
MhlI	GDGCHC	2 cut(s)	318, 1707
MlyI	GAGTC	4 cut(s)	551, 1274, 1700, 2046
MmeI	TCCRAC	7 cut(s)	138, 343, 930, 1001, 1022, 1341, 1769
MroI	TCCGGA	1 cut(s)	1420
MroNI	GCCGGC	1 cut(s)	975
MroXI	GAANNNNTTC	1 cut(s)	687
MseI	TTAA	1 cut(s)	1752
MslI	CAYNNNNRTG	1 cut(s)	1809
MspA1I	CMGCKG	5 cut(s)	23, 58, 588, 1716, 2022
MspR9I	CCNGG	7 cut(s)	101, 311, 434, 710, 1245, 1264, 1265
MunI	CAATTG	1 cut(s)	627
Mva1269I	GAATGC	1 cut(s)	1387
MvaI	CCWGG	1 cut(s)	434
MvnI	CGCG	2 cut(s)	1016, 1037
NaeI	GCCGGC	1 cut(s)	977
NciI	CCSGG	6 cut(s)	101, 311, 710, 1245, 1264, 1265
NdeII	GATC	7 cut(s)	748, 757, 893, 954, 994, 1167, 1924
NgoMIV	GCCGGC	1 cut(s)	975
NlaIII	CATG	6 cut(s)	135, 745, 1219, 1808, 1846, 2063
NlaIV	GGNNCC	7 cut(s)	162, 271, 996, 1103, 1209, 1242, 1946
NmuCI	GTSAC	1 cut(s)	640
NspV	TTCGAA	2 cut(s)	720, 1379
PciSI	GCTCTTC	3 cut(s)	99, 1295, 1844
PcsI	WCGNNNNNNNCGW	1 cut(s)	570
PctI	GAATGC	1 cut(s)	1387
PdiI	GCCGGC	1 cut(s)	977
PdmI	GAANNNNTTC	1 cut(s)	687
PfeI	GAWTC	4 cut(s)	4, 448, 764, 1517
PfoI	TCCNGGA	2 cut(s)	309, 708
PinAI	ACCGGT	1 cut(s)	1311
PleI	GAGTC	4 cut(s)	550, 1274, 1699, 2045
PmaCI	CACGTG	1 cut(s)	1174
PmlI	CACGTG	1 cut(s)	1174
PpsI	GAGTC	4 cut(s)	550, 1274, 1699, 2045
Ppu21I	YACGTR	1 cut(s)	1174
PshAI	GACNNNNGTC	2 cut(s)	1017, 1038
Psp124BI	GAGCTC	2 cut(s)	318, 1707
Psp6I	CCWGG	1 cut(s)	432
PspCI	CACGTG	1 cut(s)	1174
PspFI	CCCAGC	1 cut(s)	8
PspGI	CCWGG	1 cut(s)	432
PspN4I	GGNNCC	7 cut(s)	162, 271, 996, 1103, 1209, 1242, 1946
PspPI	GGNCC	8 cut(s)	67, 237, 270, 530, 1086, 1255, 1314, 2052
PstNI	CAGNNNCTG	2 cut(s)	810, 868
PsuI	RGATCY	2 cut(s)	757, 994
PvuII	CAGCTG	3 cut(s)	23, 58, 2022
RsaI	GTAC	3 cut(s)	1103, 2085, 2096
RsaNI	GTAC	3 cut(s)	1102, 2084, 2095
RseI	CAYNNNNRTG	1 cut(s)	1809
SacI	GAGCTC	2 cut(s)	318, 1707
SapI	GCTCTTC	3 cut(s)	99, 1295, 1844
SaqAI	TTAA	1 cut(s)	1752
Sau3AI	GATC	7 cut(s)	748, 757, 893, 954, 994, 1167, 1924
Sau96I	GGNCC	8 cut(s)	67, 237, 270, 530, 1086, 1255, 1314, 2052
SchI	GAGTC	4 cut(s)	551, 1274, 1700, 2046
ScrFI	CCNGG	7 cut(s)	101, 311, 434, 710, 1245, 1264, 1265
SduI	GDGCHC	2 cut(s)	318, 1707
SfaNI	GCATC	4 cut(s)	1119, 1128, 1172, 1532
SfuI	TTCGAA	2 cut(s)	720, 1379
SinI	GGWCC	3 cut(s)	530, 1314, 2052
SmaI	CCCGGG	1 cut(s)	1265
SmiMI	CAYNNNNRTG	1 cut(s)	1809
SspMI	CTAG	1 cut(s)	1785
SstI	GAGCTC	2 cut(s)	318, 1707
StyD4I	CCNGG	7 cut(s)	99, 309, 432, 708, 1243, 1262, 1263
StyI	CCWWGG	3 cut(s)	546, 1398, 1743
TaaI	ACNGT	4 cut(s)	51, 933, 1240, 1417
TaiI	ACGT	3 cut(s)	399, 1121, 1176
TatI	WGTACW	1 cut(s)	2094
TfiI	GAWTC	4 cut(s)	4, 448, 764, 1517
Tru1I	TTAA	1 cut(s)	1752
Tru9I	TTAA	1 cut(s)	1752
TscAI	CASTG	2 cut(s)	587, 1660
TseFI	GTSAC	1 cut(s)	640
Tsp45I	GTSAC	1 cut(s)	640
TspDTI	ATGAA	3 cut(s)	17, 758, 1232
TspGWI	ACGGA	5 cut(s)	456, 460, 1007, 1957, 1998
TspMI	CCCGGG	1 cut(s)	1263
TspRI	CASTG	2 cut(s)	587, 1660
VpaK11BI	GGWCC	3 cut(s)	530, 1314, 2052
XapI	RAATTY	7 cut(s)	499, 612, 686, 1194, 1219, 1442, 1456
XcmI	CCANNNNNNNNNTGG	1 cut(s)	907
XmaI	CCCGGG	1 cut(s)	1263
XmnI	GAANNNNTTC	1 cut(s)	687
XspI	CTAG	1 cut(s)	1785

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.