MD04G1152100.v1.1

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004350
Physical Location & Seq
Forward (+)
24117722 .. 24121215
3494 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1152100.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGACGACAGAGACATCAGAGGACGCCGTACGCCGTCGCACTGCGGTTTTCGACTACCGCAAAAAGTTACTCCAGCACAAGGAGCTCGACTCCCGAGTTCGCGCAGTGAGAGAGAACTTGCGGACTTCAAGGAAAGAATTTGGGAAAACTGAAGATGATTTGAAGTCCCTTCAAAGCGTGGGACAGATTATTGGTGAAGTTCTTCGGCCTCTCGATAATGAACGCCTGATTGTGAAGGCCAGTAGTGGTCCTAGATATGTCGTTGGGTGTCGCAGTAAGGTTGACAAAGAAAAACTAGTAACTGGCACTCGCGTTGTTTTGGATATGACGACTTTGACGATCATGCGGGCTCTTCCCAGAGAAGTTGATCCCGTTGTATATAATATGCTTCATGAAGATCCTGGTAATGTTAGTTACTCGGCTGTGGGAGGACTATCAGATCAGATCCGAGAACTTAGAGAATCCATAGAACTGCCGCTAATGAATCCTGAACTTTTTCTTAGAGTGGGGATCAAACCTCCCAAGGGTGTTCTTCTGTATGGTCCTCCGGGAACAGGGAAGACATTACTAGCCAGAGCAATTGCTAGTAACATAGATGCTAACTTTTTAAAGGTTGTATCAAGTGCCATTATTGATAAATATATTGGTGAAAGTGCAAGATTGATAAGGGAAATGTTTGGTTATGCCCGTGATCACCAGCCTTGTATCATTTTTATGGATGAGGTCGATGCTATTGGTGGACGACGTTTTAGTGAAGGGACTAGTGCAGACCGAGAAATTCAGCGAACTCTCATGGAGTTGCTGAACCAGTTAGATGGATTTGAACAGCTTGGAAAGGTGAAAATGATCATGGCAACTAATAGGCCTGATGTCCTGGATCCTGCACTTCTGCGTCCTGGGCGACTCGACCGCAAGATAGAGATCCCATTGCCTAATGAGCAATCAAGAATGGAAATTCTCAAAATCCATGCCGCCGGGATTGCCAAACATGGGGAAATTGATTATGAGGCAGTTGTGAAGCTTGCTGAGGGTTTTAATGGAGCTGATCTCCGTAATGTCTGCACTGAAGCTGGGATGTCTGCAATCCGTGCAGAAAGGGATTATGTCATCCATGAAGATTTCATGAAGGCTGTACGGAAACTGAACGAAGCAAAGAAACTTGAATCTAGTTCCCACTACAACGCAGATTTTGGGAAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005838 GO:0005886 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0022624 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

401

Amino Acids

44.92

Weight (kDa)

8.25

Isoelectric Point (pI)

35.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 64 - 119 9.9e-14 Proteasomal ATPase OB C-terminal domain
AAA PF00004 177 - 310 1.8e-43 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 333 - 375 1.5e-12 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 102, 312
AciI CCGC 7 cut(s) 44, 58, 121, 346, 476, 910, 972
AclWI GGATC 7 cut(s) 362, 392, 439, 518, 872, 885, 916
AcsI RAATTY 3 cut(s) 137, 777, 954
AcuI CTGAAG 2 cut(s) 171, 1086
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 2 cut(s) 30, 1134
AfiI CCNNNNNNNGG 4 cut(s) 79, 524, 554, 990
AgsI TTSAA 5 cut(s) 129, 163, 173, 824, 1163
AhlI ACTAGT 2 cut(s) 295, 761
AjnI CCWGG 3 cut(s) 400, 873, 895
AluBI AGCT 5 cut(s) 85, 829, 1021, 1043, 1070
AluI AGCT 5 cut(s) 85, 829, 1021, 1043, 1070
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 1 cut(s) 5
AlwI GGATC 7 cut(s) 362, 392, 439, 518, 872, 885, 916
Ama87I CYCGRG 1 cut(s) 93
AoxI GGCC 3 cut(s) 206, 237, 863
ApoI RAATTY 3 cut(s) 137, 777, 954
Asp700I GAANNNNTTC 2 cut(s) 201, 495
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 2 cut(s) 248, 542
AsuC2I CCSGG 2 cut(s) 549, 976
AsuHPI GGTGA 4 cut(s) 206, 659, 686, 850
AvaI CYCGRG 1 cut(s) 93
AvaII GGWCC 2 cut(s) 248, 542
BamHI GGATCC 1 cut(s) 877
BanII GRGCYC 2 cut(s) 87, 352
BbsI GAAGAC 1 cut(s) 566
Bbv12I GWGCWC 1 cut(s) 87
BbvCI CCTCAGC 1 cut(s) 1026
BccI CCATC 1 cut(s) 809
BceAI ACGGC 2 cut(s) 11, 18
BciT130I CCWGG 3 cut(s) 402, 875, 897
BclI TGATCA 2 cut(s) 691, 846
BcnI CCSGG 2 cut(s) 549, 976
BcoDI GTCTC 1 cut(s) 5
BcuI ACTAGT 2 cut(s) 295, 761
BfaI CTAG 6 cut(s) 252, 296, 569, 585, 762, 1167
BisI GCNGC 2 cut(s) 476, 972
BlsI GCNGC 2 cut(s) 477, 973
Bme1390I CCNGG 5 cut(s) 402, 549, 875, 897, 976
Bme18I GGWCC 2 cut(s) 248, 542
BmeT110I CYCGRG 1 cut(s) 93
BmgT120I GGNCC 2 cut(s) 248, 542
BmiI GGNNCC 1 cut(s) 879
BmrFI CCNGG 5 cut(s) 402, 549, 875, 897, 976
BmsI GCATC 2 cut(s) 586, 718
BpiI GAAGAC 1 cut(s) 566
BplI GAGNNNNNCTC 4 cut(s) 74, 106, 1032, 1064
BpmI CTGGAG 1 cut(s) 56
Bpu10I CCTNAGC 1 cut(s) 1026
BpuMI CCSGG 2 cut(s) 549, 976
BsaBI GATNNNNATC 1 cut(s) 920
BsaHI GRCGYC 1 cut(s) 24
BsaJI CCNNGG 2 cut(s) 522, 896
Bsc4I CCNNNNNNNGG 4 cut(s) 79, 524, 554, 990
Bse1I ACTGG 3 cut(s) 240, 307, 808
Bse3DI GCAATG 1 cut(s) 926
Bse8I GATNNNNATC 1 cut(s) 920
BseBI CCWGG 3 cut(s) 402, 875, 897
BseDI CCNNGG 2 cut(s) 522, 896
BseGI GGATG 3 cut(s) 724, 1080, 1107
BseJI GATNNNNATC 1 cut(s) 920
BseLI CCNNNNNNNGG 4 cut(s) 79, 524, 554, 990
BseMI GCAATG 1 cut(s) 926
BseMII CTCAG 1 cut(s) 1017
BseNI ACTGG 3 cut(s) 240, 307, 808
BseYI CCCAGC 1 cut(s) 1070
BsgI GTGCAG 4 cut(s) 786, 867, 1045, 1110
Bsh1236I CGCG 2 cut(s) 102, 312
Bsh1285I CGRYCG 1 cut(s) 910
BshFI GGCC 3 cut(s) 208, 239, 865
BsiEI CGRYCG 1 cut(s) 910
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 93
BsiSI CCGG 2 cut(s) 548, 975
BsiWI CGTACG 1 cut(s) 28
BslFI GGGAC 3 cut(s) 151, 195, 772
BslI CCNNNNNNNGG 4 cut(s) 79, 524, 554, 990
BsmAI GTCTC 1 cut(s) 5
BsmFI GGGAC 3 cut(s) 151, 195, 772
BsnI GGCC 3 cut(s) 208, 239, 865
BsoBI CYCGRG 1 cut(s) 93
Bsp1286I GDGCHC 2 cut(s) 87, 352
BspACI CCGC 7 cut(s) 44, 58, 121, 346, 476, 910, 972
BspANI GGCC 3 cut(s) 208, 239, 865
BspCNI CTCAG 1 cut(s) 1018
BspFNI CGCG 2 cut(s) 102, 312
BspHI TCATGA 2 cut(s) 391, 1122
BspLI GGNNCC 1 cut(s) 879
BspPI GGATC 7 cut(s) 362, 392, 439, 518, 872, 885, 916
BspQI GCTCTTC 1 cut(s) 357
BsrDI GCAATG 1 cut(s) 926
BsrI ACTGG 3 cut(s) 240, 307, 808
BssECI CCNNGG 2 cut(s) 522, 896
BssNI GRCGYC 1 cut(s) 24
BssT1I CCWWGG 1 cut(s) 522
Bst2UI CCWGG 3 cut(s) 402, 875, 897
Bst6I CTCTTC 1 cut(s) 357
BstACI GRCGYC 1 cut(s) 24
BstAPI GCANNNNNTGC 1 cut(s) 1088
BstC8I GCNNGC 2 cut(s) 348, 1023
BstDEI CTNAG 3 cut(s) 455, 500, 1026
BstF5I GGATG 3 cut(s) 724, 1080, 1107
BstFNI CGCG 2 cut(s) 102, 312
BstHHI GCGC 1 cut(s) 104
BstMAI GTCTC 1 cut(s) 5
BstMCI CGRYCG 1 cut(s) 910
BstMWI GCNNNNNNNGC 5 cut(s) 82, 898, 937, 980, 1088
BstNI CCWGG 3 cut(s) 402, 875, 897
BstSCI CCNGG 5 cut(s) 400, 547, 873, 895, 974
BstUI CGCG 2 cut(s) 102, 312
BstV2I GAAGAC 1 cut(s) 566
BstX2I RGATCY 4 cut(s) 397, 444, 877, 921
BstXI CCANNNNNNTGG 1 cut(s) 815
BstYI RGATCY 4 cut(s) 397, 444, 877, 921
BsuRI GGCC 3 cut(s) 208, 239, 865
BtsCI GGATG 3 cut(s) 724, 1080, 1107
BtsI GCAGTG 2 cut(s) 39, 111
BtsIMutI CAGTG 3 cut(s) 39, 111, 1062
Cac8I GCNNGC 2 cut(s) 348, 1023
CciI TCATGA 2 cut(s) 391, 1122
CfoI GCGC 1 cut(s) 104
Cfr13I GGNCC 2 cut(s) 248, 542
CseI GACGC 2 cut(s) 32, 881
Csp6I GTAC 2 cut(s) 29, 1133
CviAII CATG 8 cut(s) 343, 392, 793, 850, 968, 989, 1112, 1123
CviQI GTAC 2 cut(s) 29, 1133
DdeI CTNAG 3 cut(s) 455, 500, 1026
DraI TTTAAA 1 cut(s) 609
Eam1104I CTCTTC 1 cut(s) 357
EarI CTCTTC 1 cut(s) 357
Ecl136II GAGCTC 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 522
Eco147I AGGCCT 1 cut(s) 865
Eco24I GRGCYC 2 cut(s) 87, 352
Eco47I GGWCC 2 cut(s) 248, 542
Eco53kI GAGCTC 1 cut(s) 85
Eco57I CTGAAG 2 cut(s) 171, 1086
Eco88I CYCGRG 1 cut(s) 93
EcoICRI GAGCTC 1 cut(s) 85
EcoRII CCWGG 3 cut(s) 400, 873, 895
EcoT14I CCWWGG 1 cut(s) 522
EcoT38I GRGCYC 2 cut(s) 87, 352
ErhI CCWWGG 1 cut(s) 522
FaeI CATG 8 cut(s) 346, 395, 796, 853, 971, 992, 1115, 1126
FaqI GGGAC 3 cut(s) 151, 195, 772
FatI CATG 8 cut(s) 342, 391, 792, 849, 967, 988, 1111, 1122
FauI CCCGC 1 cut(s) 339
FbaI TGATCA 2 cut(s) 691, 846
Fnu4HI GCNGC 2 cut(s) 476, 972
FokI GGATG 3 cut(s) 731, 1087, 1094
FriOI GRGCYC 2 cut(s) 87, 352
Fsp4HI GCNGC 2 cut(s) 476, 972
FspBI CTAG 6 cut(s) 252, 296, 569, 585, 762, 1167
GlaI GCGC 1 cut(s) 103
GluI GCNGC 2 cut(s) 476, 972
GsaI CCCAGC 1 cut(s) 1074
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 3 cut(s) 208, 239, 865
HapII CCGG 2 cut(s) 548, 975
HgaI GACGC 2 cut(s) 32, 881
HhaI GCGC 1 cut(s) 104
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 8 cut(s) 346, 395, 796, 853, 971, 992, 1115, 1126
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 1 cut(s) 1019
HinfI GANTC 5 cut(s) 89, 461, 484, 903, 1163
HpaII CCGG 2 cut(s) 548, 975
HphI GGTGA 4 cut(s) 206, 659, 686, 850
Hpy166II GTNNAC 2 cut(s) 283, 740
Hpy188I TCNGA 4 cut(s) 19, 439, 444, 449
Hpy188III TCNNGA 6 cut(s) 93, 212, 392, 488, 945, 1123
Hpy8I GTNNAC 2 cut(s) 283, 740
Hpy99I CGWCG 2 cut(s) 39, 747
HpyAV CCTTC 5 cut(s) 179, 229, 749, 1120, 1189
HpyCH4IV ACGT 1 cut(s) 745
HpyCH4V TGCA 6 cut(s) 656, 767, 884, 1062, 1082, 1091
HpyF10VI GCNNNNNNNGC 5 cut(s) 82, 898, 937, 980, 1088
HpyF3I CTNAG 3 cut(s) 455, 500, 1026
HpySE526I ACGT 1 cut(s) 745
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 8 cut(s) 346, 395, 796, 853, 971, 992, 1115, 1126
HspAI GCGC 1 cut(s) 102
Ksp22I TGATCA 2 cut(s) 691, 846
LguI GCTCTTC 1 cut(s) 357
LmnI GCTCC 2 cut(s) 82, 1040
LweI GCATC 2 cut(s) 586, 718
MaeI CTAG 6 cut(s) 252, 296, 569, 585, 762, 1167
MaeII ACGT 1 cut(s) 745
MaeIII GTNAC 4 cut(s) 66, 298, 413, 587
MboII GAAGA 7 cut(s) 164, 194, 344, 407, 524, 571, 1127
MfeI CAATTG 1 cut(s) 579
MflI RGATCY 4 cut(s) 397, 444, 877, 921
MhlI GDGCHC 2 cut(s) 87, 352
MluCI AATT 5 cut(s) 137, 579, 777, 954, 996
MlyI GAGTC 2 cut(s) 83, 897
MnlI CCTC 8 cut(s) 13, 219, 422, 528, 555, 715, 1000, 1021
MroXI GAANNNNTTC 2 cut(s) 201, 495
MseI TTAA 2 cut(s) 608, 1035
MslI CAYNNNNRTG 1 cut(s) 713
MspI CCGG 2 cut(s) 548, 975
MspR9I CCNGG 5 cut(s) 402, 549, 875, 897, 976
MunI CAATTG 1 cut(s) 579
MvaI CCWGG 3 cut(s) 402, 875, 897
MvnI CGCG 2 cut(s) 102, 312
MwoI GCNNNNNNNGC 5 cut(s) 82, 898, 937, 980, 1088
NciI CCSGG 2 cut(s) 549, 976
NlaIII CATG 8 cut(s) 346, 395, 796, 853, 971, 992, 1115, 1126
NlaIV GGNNCC 1 cut(s) 879
NmeAIII GCCGAG 1 cut(s) 398
PagI TCATGA 2 cut(s) 391, 1122
PceI AGGCCT 1 cut(s) 865
PciSI GCTCTTC 1 cut(s) 357
PcsI WCGNNNNNNNCGW 1 cut(s) 335
PdmI GAANNNNTTC 2 cut(s) 201, 495
PfeI GAWTC 3 cut(s) 461, 484, 1163
Pfl23II CGTACG 1 cut(s) 28
PfoI TCCNGGA 2 cut(s) 547, 873
PkrI GCNGC 2 cut(s) 477, 973
PleI GAGTC 2 cut(s) 83, 897
PpsI GAGTC 2 cut(s) 83, 897
Psp124BI GAGCTC 1 cut(s) 87
Psp6I CCWGG 3 cut(s) 400, 873, 895
PspFI CCCAGC 1 cut(s) 1070
PspGI CCWGG 3 cut(s) 400, 873, 895
PspLI CGTACG 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 879
PspPI GGNCC 2 cut(s) 248, 542
PsuI RGATCY 4 cut(s) 397, 444, 877, 921
RsaI GTAC 2 cut(s) 30, 1134
RsaNI GTAC 2 cut(s) 29, 1133
RseI CAYNNNNRTG 1 cut(s) 713
SacI GAGCTC 1 cut(s) 87
SapI GCTCTTC 1 cut(s) 357
SaqAI TTAA 2 cut(s) 608, 1035
SatI GCNGC 2 cut(s) 476, 972
Sau96I GGNCC 2 cut(s) 248, 542
SchI GAGTC 2 cut(s) 83, 897
ScrFI CCNGG 5 cut(s) 402, 549, 875, 897, 976
SduI GDGCHC 2 cut(s) 87, 352
SfaNI GCATC 2 cut(s) 586, 718
SinI GGWCC 2 cut(s) 248, 542
SmiMI CAYNNNNRTG 1 cut(s) 713
SpeI ACTAGT 2 cut(s) 295, 761
Sse9I AATT 5 cut(s) 137, 579, 777, 954, 996
SseBI AGGCCT 1 cut(s) 865
SsiI CCGC 7 cut(s) 44, 58, 121, 346, 476, 910, 972
SspMI CTAG 6 cut(s) 252, 296, 569, 585, 762, 1167
SstI GAGCTC 1 cut(s) 87
StuI AGGCCT 1 cut(s) 865
StyD4I CCNGG 5 cut(s) 400, 547, 873, 895, 974
StyI CCWWGG 1 cut(s) 522
TaiI ACGT 1 cut(s) 748
TaqI TCGA 5 cut(s) 51, 87, 213, 726, 906
TaqII GACCGA 1 cut(s) 786
TasI AATT 5 cut(s) 137, 579, 777, 954, 996
TauI GCSGC 2 cut(s) 478, 974
TfiI GAWTC 3 cut(s) 461, 484, 1163
Tru1I TTAA 2 cut(s) 608, 1035
Tru9I TTAA 2 cut(s) 608, 1035
TscAI CASTG 3 cut(s) 46, 111, 1069
TspDTI ATGAA 7 cut(s) 234, 380, 408, 497, 1111, 1128, 1139
TspGWI ACGGA 3 cut(s) 1040, 1076, 1150
TspRI CASTG 3 cut(s) 46, 111, 1069
VpaK11BI GGWCC 2 cut(s) 248, 542
XapI RAATTY 3 cut(s) 137, 777, 954
XmnI GAANNNNTTC 2 cut(s) 201, 495
XspI CTAG 6 cut(s) 252, 296, 569, 585, 762, 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.