pycom05g05180

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0004350
Physical Location & Seq
Forward (+)
6170948 .. 6173220
2273 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g05180.4

Sequence Viewer

Length: 1044 bp
ATGACGACTGAGACAGAAGACGCCGTACGACGTCGCACAGCGGTTGCCGACTACCGCAAAAGGTTACTCCAGCACAAGGAGCTCGACTCCCGAGTCCGCGCAGTGAGAGAGAACTTGCGGTCAGCAAAGAAAGAATTTGGGAAAACTGAAGATGATTTGAAGTCCCTTCAAAGTGTTGGACAGATTATTGGTGAAGTTCTCAGGCCTCTTGATAACGAACGCCTAATTGTGAAGGCAAGTAGTGGCCCGAGGTATGTCGTTGGGTGTCGCAGTAAAGTTGACAAAGAAAAACTAACAGCTGGAACGCGTGTTGTTTTGGATATGACTACCTTGACTATCATGCGGGCTCTACCCAGAGAAGTTGATCCGGTTGTATATAACATGCTTCATGAAGATCCTGGTAATGTTAGCTACTCAGCCGTTGGAGGACTATCTGATCAAATCCGAGAGCTTAGGGAGTCCATTGAACTGCCTCTAATGAACCCTGAGCTCTTCCTTAGGGTGGGGATCAAACCTCCCAAGGGTGTTCTTCTGTATGGACCTCCAGGAACAGGGAAGACATTGCTAGCCAGAGCAATTGCTAGCAACATAGATGCTAACTTTCTTAAGGTTGTATCAAGTGCCATTATTGACAAATATATCGGTGAAAGTGCAAGATTGATAAGGGAAATGTTTGGTTATGCCCGTGATCGATCTCACCAGCCATGTATCATTTTTATGGATGAGATAGATGCTATTGGTGGACGGCGTTTTAGTGAAGGGACTAGTGCAGACCGAGAAATTCAGCGAACACTCATGGAGTTGCTTAATCAGTTAGATGGGTTTGATCAGCTCGGAAAGGTGAAAATGATCATGGCAACCAATAGGCCTGATGTACTGGATCCAGCACTTCTCCGCCCTGGGCGACTAGACCGCAAGATAGAGATCCCATTGCCCAATGAGCAATCAAGAATGGAAATTCTCAAAATCCATGCTGCTGGGATAGCCAAACATGGGGACATTGATTATGAGGCAGTTGTGAAGCTTGCTGAGGTAAGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005838 GO:0005886 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0022624 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

348

Amino Acids

38.93

Weight (kDa)

9.05

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AatII GACGTC 1 cut(s) 34
AccII CGCG 2 cut(s) 99, 307
AciI CCGC 7 cut(s) 41, 55, 97, 118, 343, 895, 913
AclWI GGATC 6 cut(s) 359, 389, 515, 875, 888, 919
AcsI RAATTY 3 cut(s) 134, 780, 957
AcuI CTGAAG 1 cut(s) 168
AcyI GRCGYC 2 cut(s) 21, 31
AfaI GTAC 2 cut(s) 27, 876
AfiI CCNNNNNNNGG 5 cut(s) 76, 502, 521, 551, 993
AflII CTTAAG 1 cut(s) 605
AflIII ACRYGT 1 cut(s) 305
AgsI TTSAA 3 cut(s) 160, 170, 467
AhlI ACTAGT 1 cut(s) 764
AjnI CCWGG 3 cut(s) 397, 544, 898
AluBI AGCT 7 cut(s) 82, 299, 411, 451, 490, 832, 1024
AluI AGCT 7 cut(s) 82, 299, 411, 451, 490, 832, 1024
Alw21I GWGCWC 2 cut(s) 84, 492
Alw26I GTCTC 1 cut(s) 5
AlwI GGATC 6 cut(s) 359, 389, 515, 875, 888, 919
Ama87I CYCGRG 2 cut(s) 90, 247
AoxI GGCC 3 cut(s) 203, 244, 866
ApeKI GCWGC 1 cut(s) 974
ApoI RAATTY 3 cut(s) 134, 780, 957
AspLEI GCGC 1 cut(s) 101
AspS9I GGNCC 2 cut(s) 245, 539
AsuHPI GGTGA 4 cut(s) 203, 656, 689, 853
AsuNHI GCTAGC 2 cut(s) 565, 581
AvaI CYCGRG 2 cut(s) 90, 247
AvaII GGWCC 1 cut(s) 539
AxyI CCTNAGG 1 cut(s) 497
BamHI GGATCC 1 cut(s) 880
BanII GRGCYC 3 cut(s) 84, 349, 492
BarI GAAGNNNNNNTAC 2 cut(s) 9, 41
BbsI GAAGAC 2 cut(s) 24, 563
Bbv12I GWGCWC 2 cut(s) 84, 492
BbvCI CCTCAGC 1 cut(s) 1029
BbvI GCAGC 1 cut(s) 961
BccI CCATC 1 cut(s) 812
BceAI ACGGC 3 cut(s) 8, 404, 761
BciT130I CCWGG 3 cut(s) 399, 546, 900
BclI TGATCA 3 cut(s) 436, 826, 849
BcoDI GTCTC 1 cut(s) 5
BcuI ACTAGT 1 cut(s) 764
BfaI CTAG 4 cut(s) 566, 582, 765, 908
BfrI CTTAAG 1 cut(s) 605
BisI GCNGC 1 cut(s) 975
BlsI GCNGC 1 cut(s) 976
Bme1390I CCNGG 3 cut(s) 399, 546, 900
Bme18I GGWCC 1 cut(s) 539
BmeT110I CYCGRG 2 cut(s) 90, 247
BmgT120I GGNCC 2 cut(s) 245, 539
BmiI GGNNCC 1 cut(s) 882
BmrFI CCNGG 3 cut(s) 399, 546, 900
BmsI GCATC 2 cut(s) 583, 721
BmtI GCTAGC 2 cut(s) 569, 585
BpiI GAAGAC 2 cut(s) 24, 563
BplI GAGNNNNNCTC 2 cut(s) 71, 103
BpmI CTGGAG 2 cut(s) 53, 528
Bpu10I CCTNAGC 3 cut(s) 452, 486, 1029
Bsa29I ATCGAT 1 cut(s) 691
BsaBI GATNNNNATC 1 cut(s) 923
BsaHI GRCGYC 2 cut(s) 21, 31
BsaJI CCNNGG 4 cut(s) 248, 519, 898, 899
BsaWI WCCGGW 1 cut(s) 367
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 502, 521, 551, 993
Bse1I ACTGG 1 cut(s) 882
Bse21I CCTNAGG 1 cut(s) 497
Bse3DI GCAATG 2 cut(s) 560, 929
Bse8I GATNNNNATC 1 cut(s) 923
BseBI CCWGG 3 cut(s) 399, 546, 900
BseCI ATCGAT 1 cut(s) 691
BseDI CCNNGG 4 cut(s) 248, 519, 898, 899
BseGI GGATG 1 cut(s) 727
BseJI GATNNNNATC 1 cut(s) 923
BseLI CCNNNNNNNGG 5 cut(s) 76, 502, 521, 551, 993
BseMI GCAATG 2 cut(s) 560, 929
BseMII CTCAG 4 cut(s) 214, 429, 477, 1020
BseNI ACTGG 1 cut(s) 882
BseXI GCAGC 1 cut(s) 961
BseYI CCCAGC 1 cut(s) 977
BsgI GTGCAG 1 cut(s) 789
Bsh1236I CGCG 2 cut(s) 99, 307
BshFI GGCC 3 cut(s) 205, 246, 868
BshVI ATCGAT 1 cut(s) 691
BsiHKAI GWGCWC 2 cut(s) 84, 492
BsiHKCI CYCGRG 2 cut(s) 90, 247
BsiSI CCGG 1 cut(s) 368
BsiWI CGTACG 1 cut(s) 25
BslFI GGGAC 3 cut(s) 148, 775, 1010
BslI CCNNNNNNNGG 5 cut(s) 76, 502, 521, 551, 993
BsmAI GTCTC 1 cut(s) 5
BsmFI GGGAC 3 cut(s) 148, 775, 1010
BsnI GGCC 3 cut(s) 205, 246, 868
BsoBI CYCGRG 2 cut(s) 90, 247
Bsp1286I GDGCHC 3 cut(s) 84, 349, 492
BspACI CCGC 7 cut(s) 41, 55, 97, 118, 343, 895, 913
BspANI GGCC 3 cut(s) 205, 246, 868
BspCNI CTCAG 4 cut(s) 213, 428, 478, 1021
BspDI ATCGAT 1 cut(s) 691
BspFNI CGCG 2 cut(s) 99, 307
BspHI TCATGA 1 cut(s) 388
BspLI GGNNCC 1 cut(s) 882
BspOI GCTAGC 2 cut(s) 569, 585
BspPI GGATC 6 cut(s) 359, 389, 515, 875, 888, 919
BspQI GCTCTTC 1 cut(s) 497
BspTI CTTAAG 1 cut(s) 605
BsrDI GCAATG 2 cut(s) 560, 929
BsrI ACTGG 1 cut(s) 882
BssECI CCNNGG 4 cut(s) 248, 519, 898, 899
BssNI GRCGYC 2 cut(s) 21, 31
BssT1I CCWWGG 1 cut(s) 519
Bst2UI CCWGG 3 cut(s) 399, 546, 900
Bst6I CTCTTC 1 cut(s) 497
BstACI GRCGYC 2 cut(s) 21, 31
BstAFI CTTAAG 1 cut(s) 605
BstC8I GCNNGC 4 cut(s) 345, 567, 583, 1026
BstDEI CTNAG 7 cut(s) 9, 200, 415, 452, 486, 497, 1029
BstF5I GGATG 1 cut(s) 727
BstFNI CGCG 2 cut(s) 99, 307
BstHHI GCGC 1 cut(s) 101
BstMAI GTCTC 1 cut(s) 5
BstMWI GCNNNNNNNGC 3 cut(s) 79, 940, 983
BstNI CCWGG 3 cut(s) 399, 546, 900
BstNSI RCATGY 1 cut(s) 385
BstSCI CCNGG 3 cut(s) 397, 544, 898
BstUI CGCG 2 cut(s) 99, 307
BstV1I GCAGC 1 cut(s) 961
BstV2I GAAGAC 2 cut(s) 24, 563
BstX2I RGATCY 3 cut(s) 394, 880, 924
BstXI CCANNNNNNTGG 1 cut(s) 977
BstYI RGATCY 3 cut(s) 394, 880, 924
Bsu15I ATCGAT 1 cut(s) 691
Bsu36I CCTNAGG 1 cut(s) 497
BsuRI GGCC 3 cut(s) 205, 246, 868
BsuTUI ATCGAT 1 cut(s) 691
BtsCI GGATG 1 cut(s) 727
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 108
Cac8I GCNNGC 4 cut(s) 345, 567, 583, 1026
CciI TCATGA 1 cut(s) 388
CfoI GCGC 1 cut(s) 101
Cfr13I GGNCC 2 cut(s) 245, 539
ClaI ATCGAT 1 cut(s) 691
CseI GACGC 1 cut(s) 29
Csp6I GTAC 2 cut(s) 26, 875
CviAII CATG 8 cut(s) 340, 382, 389, 705, 796, 853, 971, 992
CviQI GTAC 2 cut(s) 26, 875
DdeI CTNAG 7 cut(s) 9, 200, 415, 452, 486, 497, 1029
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
Eam1104I CTCTTC 1 cut(s) 497
EarI CTCTTC 1 cut(s) 497
EciI GGCGGA 1 cut(s) 884
Ecl136II GAGCTC 2 cut(s) 82, 490
Eco130I CCWWGG 1 cut(s) 519
Eco147I AGGCCT 2 cut(s) 205, 868
Eco24I GRGCYC 3 cut(s) 84, 349, 492
Eco47I GGWCC 1 cut(s) 539
Eco53kI GAGCTC 2 cut(s) 82, 490
Eco57I CTGAAG 1 cut(s) 168
Eco81I CCTNAGG 1 cut(s) 497
Eco88I CYCGRG 2 cut(s) 90, 247
EcoICRI GAGCTC 2 cut(s) 82, 490
EcoRII CCWGG 3 cut(s) 397, 544, 898
EcoT14I CCWWGG 1 cut(s) 519
EcoT38I GRGCYC 3 cut(s) 84, 349, 492
ErhI CCWWGG 1 cut(s) 519
FaeI CATG 8 cut(s) 343, 385, 392, 708, 799, 856, 974, 995
FaqI GGGAC 3 cut(s) 148, 775, 1010
FatI CATG 8 cut(s) 339, 381, 388, 704, 795, 852, 970, 991
FauI CCCGC 1 cut(s) 336
FbaI TGATCA 3 cut(s) 436, 826, 849
Fnu4HI GCNGC 1 cut(s) 975
FokI GGATG 1 cut(s) 734
FriOI GRGCYC 3 cut(s) 84, 349, 492
Fsp4HI GCNGC 1 cut(s) 975
FspBI CTAG 4 cut(s) 566, 582, 765, 908
GlaI GCGC 1 cut(s) 100
GluI GCNGC 1 cut(s) 975
GsaI CCCAGC 1 cut(s) 981
GsuI CTGGAG 2 cut(s) 53, 528
HaeIII GGCC 3 cut(s) 205, 246, 868
HapII CCGG 1 cut(s) 368
HgaI GACGC 1 cut(s) 29
HhaI GCGC 1 cut(s) 101
Hin1I GRCGYC 2 cut(s) 21, 31
Hin1II CATG 8 cut(s) 343, 385, 392, 708, 799, 856, 974, 995
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 1 cut(s) 1022
HinfI GANTC 3 cut(s) 86, 93, 458
HpaII CCGG 1 cut(s) 368
HphI GGTGA 4 cut(s) 203, 656, 689, 853
Hpy166II GTNNAC 2 cut(s) 280, 743
Hpy188I TCNGA 3 cut(s) 436, 446, 836
Hpy188III TCNNGA 4 cut(s) 90, 209, 389, 948
Hpy8I GTNNAC 2 cut(s) 280, 743
Hpy99I CGWCG 2 cut(s) 33, 36
HpyAV CCTTC 3 cut(s) 176, 226, 752
HpyCH4IV ACGT 1 cut(s) 31
HpyCH4V TGCA 2 cut(s) 653, 770
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 940, 983
HpyF3I CTNAG 7 cut(s) 9, 200, 415, 452, 486, 497, 1029
HpySE526I ACGT 1 cut(s) 31
Hsp92I GRCGYC 2 cut(s) 21, 31
Hsp92II CATG 8 cut(s) 343, 385, 392, 708, 799, 856, 974, 995
HspAI GCGC 1 cut(s) 99
Ksp22I TGATCA 3 cut(s) 436, 826, 849
LguI GCTCTTC 1 cut(s) 497
LmnI GCTCC 1 cut(s) 79
Lsp1109I GCAGC 1 cut(s) 961
LweI GCATC 2 cut(s) 583, 721
MaeI CTAG 4 cut(s) 566, 582, 765, 908
MaeII ACGT 1 cut(s) 31
MaeIII GTNAC 1 cut(s) 63
MboII GAAGA 6 cut(s) 29, 161, 404, 484, 521, 568
MfeI CAATTG 1 cut(s) 576
MflI RGATCY 3 cut(s) 394, 880, 924
MhlI GDGCHC 3 cut(s) 84, 349, 492
MluCI AATT 5 cut(s) 134, 225, 576, 780, 957
MluI ACGCGT 1 cut(s) 305
MlyI GAGTC 3 cut(s) 80, 102, 467
MmeI TCCRAC 2 cut(s) 157, 403
MnlI CCTC 8 cut(s) 216, 243, 419, 483, 525, 552, 1003, 1024
MseI TTAA 3 cut(s) 606, 807, 1042
MslI CAYNNNNRTG 1 cut(s) 716
MspA1I CMGCKG 2 cut(s) 41, 299
MspCI CTTAAG 1 cut(s) 605
MspI CCGG 1 cut(s) 368
MspR9I CCNGG 3 cut(s) 399, 546, 900
MunI CAATTG 1 cut(s) 576
MvaI CCWGG 3 cut(s) 399, 546, 900
MvnI CGCG 2 cut(s) 99, 307
MwoI GCNNNNNNNGC 3 cut(s) 79, 940, 983
NheI GCTAGC 2 cut(s) 565, 581
NlaIII CATG 8 cut(s) 343, 385, 392, 708, 799, 856, 974, 995
NlaIV GGNNCC 1 cut(s) 882
NspI RCATGY 1 cut(s) 385
PagI TCATGA 1 cut(s) 388
PasI CCCWGGG 1 cut(s) 899
PceI AGGCCT 2 cut(s) 205, 868
PciSI GCTCTTC 1 cut(s) 497
Pfl23II CGTACG 1 cut(s) 25
PfoI TCCNGGA 1 cut(s) 544
PkrI GCNGC 1 cut(s) 976
PleI GAGTC 3 cut(s) 80, 101, 466
PpsI GAGTC 3 cut(s) 80, 101, 466
Psp124BI GAGCTC 2 cut(s) 84, 492
Psp6I CCWGG 3 cut(s) 397, 544, 898
PspFI CCCAGC 1 cut(s) 977
PspGI CCWGG 3 cut(s) 397, 544, 898
PspLI CGTACG 1 cut(s) 25
PspN4I GGNNCC 1 cut(s) 882
PspPI GGNCC 2 cut(s) 245, 539
PsuI RGATCY 3 cut(s) 394, 880, 924
PvuII CAGCTG 1 cut(s) 299
RsaI GTAC 2 cut(s) 27, 876
RsaNI GTAC 2 cut(s) 26, 875
RseI CAYNNNNRTG 1 cut(s) 716
SacI GAGCTC 2 cut(s) 84, 492
SapI GCTCTTC 1 cut(s) 497
SaqAI TTAA 3 cut(s) 606, 807, 1042
SatI GCNGC 1 cut(s) 975
Sau96I GGNCC 2 cut(s) 245, 539
SchI GAGTC 3 cut(s) 80, 102, 467
ScrFI CCNGG 3 cut(s) 399, 546, 900
SduI GDGCHC 3 cut(s) 84, 349, 492
SfaNI GCATC 2 cut(s) 583, 721
SinI GGWCC 1 cut(s) 539
SmiMI CAYNNNNRTG 1 cut(s) 716
SmlI CTYRAG 1 cut(s) 605
SmoI CTYRAG 1 cut(s) 605
SpeI ACTAGT 1 cut(s) 764
Sse9I AATT 5 cut(s) 134, 225, 576, 780, 957
SseBI AGGCCT 2 cut(s) 205, 868
SsiI CCGC 7 cut(s) 41, 55, 97, 118, 343, 895, 913
SspMI CTAG 4 cut(s) 566, 582, 765, 908
SstI GAGCTC 2 cut(s) 84, 492
StuI AGGCCT 2 cut(s) 205, 868
StyD4I CCNGG 3 cut(s) 397, 544, 898
StyI CCWWGG 1 cut(s) 519
TaiI ACGT 1 cut(s) 34
TaqI TCGA 2 cut(s) 84, 691
TaqII GACCGA 1 cut(s) 789
TasI AATT 5 cut(s) 134, 225, 576, 780, 957
TatI WGTACW 1 cut(s) 874
Tru1I TTAA 3 cut(s) 606, 807, 1042
Tru9I TTAA 3 cut(s) 606, 807, 1042
TscAI CASTG 1 cut(s) 108
TseI GCWGC 1 cut(s) 974
TspDTI ATGAA 3 cut(s) 377, 405, 494
TspRI CASTG 1 cut(s) 108
Vha464I CTTAAG 1 cut(s) 605
VpaK11BI GGWCC 1 cut(s) 539
XapI RAATTY 3 cut(s) 134, 780, 957
XceI RCATGY 1 cut(s) 385
XspI CTAG 4 cut(s) 566, 582, 765, 908
ZraI GACGTC 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.