RLG00000014139

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004350
Physical Location & Seq
Forward (+)
47956537 .. 47959485
2949 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014139

Sequence Viewer

Length: 1071 bp
ATGACGTCGACCGAGACAGAGGACGCCGTGCGACGTCGCTCCGCCATCACTGATTACCGGAAGAGATTACTCCAGCACAAGGAGCTCGACTCCCGAGTCCGCGCAGTGCGGGAAAACTTGCGAGGAGCTAAGAAGGAGTTTGGGAAAACTGAAGATGATTTGAAGTCGCTTCAAAGTGTTGGTCAGATTATAGGGGAAGTTCTGAGACCTCTCGATAATGAACGCTTGATAGTGAAGGCGAGTAGTGGCCCTAGGTATGTGGTCGGCTGTCGCAGTAAAGTGGACAAAGAAAAACTAACAGCGGGCACTCGGGTTGTTTTGGATATGACTACTCTGACTATCATGAGGGCTCTTCCAAGAGAGGTTGATCCAGTAGTGTATAATATGCTTCATGAAGATCCCGGTAATGTTAGCTACTCCGCTGTGGGAGGGCTAGGAGACCAGATCCGGGAGCTTAGGGAATCTATAGAGCTGCCTCTAATGAACCCTGAGCTCTTCCTTAGAGTGGGCATCAAACCACCCAAGCAACTTAGAATCGTAGGATTTTCATCAAGAAATATTCTTTCTTATTTTATTGAATTGTTCCAGGGTATCGATGCCATCGGTGGACGGCGTTTTAGTGAAGGGACCAGTGCAGACCGTGAAATCCAGCGAACACTCATGGAGTTGCTTAACCAGTTAGATGGATTTGATCAACTTGGGAAGGTGAAAATGATAATGGCAACAAATAGACCTGATGTTTTAGATCCTGCACTTCTCCGACCTGGGAGACTAGACCGGAAAATAGAGATTCCATTGCCTAATGAACAGTCAAGAATGGAAATTCTTAAAATTCATGCTGCTGGAATAGCTAAACATGGGGAAATTGATTATGAGGCAGTTGTGAAGCTTGCTGAGGGCTTTAATGGAGCAGATCTGCGGAATGTCTGCACTGAAGCTGGGATGTCTGCAATCCGTGCTGAACGCGATTATGTCATTCATGAAGACTTCATGAAGGCTGTAAGAAAATTGAACGAAGCCAAGAAGCTGGAATCGAGCGCACACTATAATGCTGATTTTGGAAAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005838 GO:0005886 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0022624 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

357

Amino Acids

40.1

Weight (kDa)

8.67

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 64 - 119 8.8e-15 Proteasomal ATPase OB C-terminal domain
AAA PF00004 195 - 266 7.9e-14 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 289 - 331 1.3e-12 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 95
AatII GACGTC 2 cut(s) 8, 37
AccI GTMKAC 1 cut(s) 8
AccII CGCG 2 cut(s) 102, 966
AciI CCGC 6 cut(s) 42, 100, 109, 302, 420, 919
AclWI GGATC 4 cut(s) 362, 392, 439, 740
AcsI RAATTY 2 cut(s) 822, 831
AcuI CTGAAG 2 cut(s) 171, 954
AcyI GRCGYC 3 cut(s) 5, 24, 34
AfiI CCNNNNNNNGG 3 cut(s) 79, 448, 505
AgsI TTSAA 4 cut(s) 163, 173, 578, 1012
AjnI CCWGG 2 cut(s) 585, 763
Alw21I GWGCWC 2 cut(s) 87, 495
Alw26I GTCTC 4 cut(s) 8, 199, 432, 763
AlwI GGATC 4 cut(s) 362, 392, 439, 740
Ama87I CYCGRG 2 cut(s) 93, 309
AoxI GGCC 1 cut(s) 247
ApeKI GCWGC 2 cut(s) 472, 839
ApoI RAATTY 2 cut(s) 822, 831
ArsI GACNNNNNNTTYG 2 cut(s) 166, 198
AspA2I CCTAGG 1 cut(s) 251
AspLEI GCGC 2 cut(s) 104, 1040
AspS9I GGNCC 2 cut(s) 248, 627
AsuC2I CCSGG 2 cut(s) 402, 449
AsuHPI GGTGA 1 cut(s) 718
AvaI CYCGRG 2 cut(s) 93, 309
AvaII GGWCC 1 cut(s) 627
AvrII CCTAGG 1 cut(s) 251
BaeGI GKGCMC 1 cut(s) 308
BanII GRGCYC 3 cut(s) 87, 352, 495
BbsI GAAGAC 1 cut(s) 990
Bbv12I GWGCWC 2 cut(s) 87, 495
BbvCI CCTCAGC 1 cut(s) 894
BbvI GCAGC 2 cut(s) 459, 826
BccI CCATC 3 cut(s) 53, 608, 677
BceAI ACGGC 2 cut(s) 11, 626
BciT130I CCWGG 2 cut(s) 587, 765
BclI TGATCA 1 cut(s) 691
BcnI CCSGG 2 cut(s) 402, 449
BcoDI GTCTC 4 cut(s) 8, 199, 432, 763
BfaI CTAG 3 cut(s) 252, 434, 773
BfmI CTRYAG 1 cut(s) 465
BglII AGATCT 1 cut(s) 913
BisI GCNGC 2 cut(s) 473, 840
BlnI CCTAGG 1 cut(s) 251
BlsI GCNGC 2 cut(s) 474, 841
Bme1390I CCNGG 4 cut(s) 402, 449, 587, 765
Bme18I GGWCC 1 cut(s) 627
BmeT110I CYCGRG 2 cut(s) 93, 309
BmgT120I GGNCC 2 cut(s) 248, 627
BmiI GGNNCC 1 cut(s) 628
BmrFI CCNGG 4 cut(s) 402, 449, 587, 765
BmsI GCATC 2 cut(s) 519, 586
BpiI GAAGAC 1 cut(s) 990
BplI GAGNNNNNCTC 2 cut(s) 74, 106
BpmI CTGGAG 1 cut(s) 56
Bpu10I CCTNAGC 3 cut(s) 455, 489, 894
BpuMI CCSGG 2 cut(s) 402, 449
Bsa29I ATCGAT 1 cut(s) 594
BsaBI GATNNNNATC 1 cut(s) 547
BsaHI GRCGYC 3 cut(s) 5, 24, 34
BsaI GGTCTC 2 cut(s) 199, 432
BsaJI CCNNGG 3 cut(s) 251, 586, 764
BsaWI WCCGGW 2 cut(s) 57, 777
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 448, 505
Bse1I ACTGG 3 cut(s) 371, 630, 676
Bse3DI GCAATG 1 cut(s) 794
Bse8I GATNNNNATC 1 cut(s) 547
BseBI CCWGG 2 cut(s) 587, 765
BseCI ATCGAT 1 cut(s) 594
BseDI CCNNGG 3 cut(s) 251, 586, 764
BseGI GGATG 1 cut(s) 948
BseJI GATNNNNATC 1 cut(s) 547
BseLI CCNNNNNNNGG 3 cut(s) 79, 448, 505
BseMI GCAATG 1 cut(s) 794
BseMII CTCAG 3 cut(s) 194, 480, 885
BseNI ACTGG 3 cut(s) 371, 630, 676
BseRI GAGGAG 1 cut(s) 138
BseSI GKGCMC 1 cut(s) 308
BseXI GCAGC 2 cut(s) 459, 826
BseYI CCCAGC 1 cut(s) 938
BsgI GTGCAG 3 cut(s) 654, 735, 913
Bsh1236I CGCG 2 cut(s) 102, 966
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 1 cut(s) 249
BshVI ATCGAT 1 cut(s) 594
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 87, 495
BsiHKCI CYCGRG 2 cut(s) 93, 309
BsiSI CCGG 4 cut(s) 58, 402, 448, 778
BslFI GGGAC 1 cut(s) 640
BslI CCNNNNNNNGG 3 cut(s) 79, 448, 505
BsmAI GTCTC 4 cut(s) 8, 199, 432, 763
BsmFI GGGAC 1 cut(s) 640
BsnI GGCC 1 cut(s) 249
Bso31I GGTCTC 2 cut(s) 199, 432
BsoBI CYCGRG 2 cut(s) 93, 309
Bsp1286I GDGCHC 4 cut(s) 87, 308, 352, 495
Bsp143I GATC 6 cut(s) 367, 397, 444, 691, 745, 913
BspACI CCGC 6 cut(s) 42, 100, 109, 302, 420, 919
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 3 cut(s) 195, 481, 886
BspDI ATCGAT 1 cut(s) 594
BspFNI CGCG 2 cut(s) 102, 966
BspHI TCATGA 4 cut(s) 342, 391, 979, 990
BspLI GGNNCC 1 cut(s) 628
BspPI GGATC 4 cut(s) 362, 392, 439, 740
BspQI GCTCTTC 2 cut(s) 357, 500
BspTNI GGTCTC 2 cut(s) 199, 432
BsrDI GCAATG 1 cut(s) 794
BsrI ACTGG 3 cut(s) 371, 630, 676
BssECI CCNNGG 3 cut(s) 251, 586, 764
BssMI GATC 6 cut(s) 367, 397, 444, 691, 745, 913
BssNI GRCGYC 3 cut(s) 5, 24, 34
BssT1I CCWWGG 1 cut(s) 251
Bst2UI CCWGG 2 cut(s) 587, 765
Bst4CI ACNGT 2 cut(s) 641, 810
Bst6I CTCTTC 3 cut(s) 56, 357, 500
BstACI GRCGYC 3 cut(s) 5, 24, 34
BstAPI GCANNNNNTGC 1 cut(s) 956
BstC8I GCNNGC 2 cut(s) 304, 891
BstDEI CTNAG 7 cut(s) 129, 203, 455, 489, 500, 530, 894
BstF5I GGATG 1 cut(s) 948
BstFNI CGCG 2 cut(s) 102, 966
BstHHI GCGC 2 cut(s) 104, 1040
BstKTI GATC 6 cut(s) 370, 400, 447, 694, 748, 916
BstMAI GTCTC 4 cut(s) 8, 199, 432, 763
BstMBI GATC 6 cut(s) 367, 397, 444, 691, 745, 913
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 3 cut(s) 82, 848, 956
BstNI CCWGG 2 cut(s) 587, 765
BstSCI CCNGG 4 cut(s) 400, 447, 585, 763
BstSFI CTRYAG 1 cut(s) 465
BstSLI GKGCMC 1 cut(s) 308
BstUI CGCG 2 cut(s) 102, 966
BstV1I GCAGC 2 cut(s) 459, 826
BstV2I GAAGAC 1 cut(s) 990
BstX2I RGATCY 4 cut(s) 397, 444, 745, 913
BstXI CCANNNNNNTGG 2 cut(s) 683, 1027
BstYI RGATCY 4 cut(s) 397, 444, 745, 913
Bsu15I ATCGAT 1 cut(s) 594
BsuRI GGCC 1 cut(s) 249
BsuTUI ATCGAT 1 cut(s) 594
BtsCI GGATG 1 cut(s) 948
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 4 cut(s) 48, 111, 637, 930
Cac8I GCNNGC 2 cut(s) 304, 891
CciI TCATGA 4 cut(s) 342, 391, 979, 990
CfoI GCGC 2 cut(s) 104, 1040
Cfr13I GGNCC 2 cut(s) 248, 627
ClaI ATCGAT 1 cut(s) 594
CseI GACGC 1 cut(s) 32
CviAII CATG 7 cut(s) 343, 392, 661, 836, 857, 980, 991
DdeI CTNAG 7 cut(s) 129, 203, 455, 489, 500, 530, 894
DpnI GATC 6 cut(s) 369, 399, 446, 693, 747, 915
DpnII GATC 6 cut(s) 367, 397, 444, 691, 745, 913
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
Eam1104I CTCTTC 3 cut(s) 56, 357, 500
EarI CTCTTC 3 cut(s) 56, 357, 500
EciI GGCGGA 1 cut(s) 31
Ecl136II GAGCTC 2 cut(s) 85, 493
Eco130I CCWWGG 1 cut(s) 251
Eco24I GRGCYC 3 cut(s) 87, 352, 495
Eco31I GGTCTC 2 cut(s) 199, 432
Eco47I GGWCC 1 cut(s) 627
Eco53kI GAGCTC 2 cut(s) 85, 493
Eco57I CTGAAG 2 cut(s) 171, 954
Eco88I CYCGRG 2 cut(s) 93, 309
EcoICRI GAGCTC 2 cut(s) 85, 493
EcoRII CCWGG 2 cut(s) 585, 763
EcoT14I CCWWGG 1 cut(s) 251
EcoT38I GRGCYC 3 cut(s) 87, 352, 495
ErhI CCWWGG 1 cut(s) 251
FaeI CATG 7 cut(s) 346, 395, 664, 839, 860, 983, 994
FaqI GGGAC 1 cut(s) 640
FatI CATG 7 cut(s) 342, 391, 660, 835, 856, 979, 990
FauI CCCGC 2 cut(s) 102, 295
FbaI TGATCA 1 cut(s) 691
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 2 cut(s) 473, 840
FokI GGATG 1 cut(s) 955
FriOI GRGCYC 3 cut(s) 87, 352, 495
Fsp4HI GCNGC 2 cut(s) 473, 840
FspBI CTAG 3 cut(s) 252, 434, 773
GlaI GCGC 2 cut(s) 103, 1039
GluI GCNGC 2 cut(s) 473, 840
GsaI CCCAGC 1 cut(s) 942
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 1 cut(s) 249
HapII CCGG 4 cut(s) 58, 402, 448, 778
HgaI GACGC 1 cut(s) 32
HhaI GCGC 2 cut(s) 104, 1040
Hin1I GRCGYC 3 cut(s) 5, 24, 34
Hin1II CATG 7 cut(s) 346, 395, 664, 839, 860, 983, 994
Hin6I GCGC 2 cut(s) 102, 1038
HinP1I GCGC 2 cut(s) 102, 1038
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HindIII AAGCTT 1 cut(s) 887
HinfI GANTC 6 cut(s) 89, 96, 461, 534, 790, 1031
HpaII CCGG 4 cut(s) 58, 402, 448, 778
HphI GGTGA 1 cut(s) 718
Hpy166II GTNNAC 3 cut(s) 9, 283, 608
Hpy188I TCNGA 4 cut(s) 186, 204, 336, 761
Hpy188III TCNNGA 8 cut(s) 93, 212, 343, 392, 552, 813, 980, 991
Hpy8I GTNNAC 3 cut(s) 9, 283, 608
Hpy99I CGWCG 3 cut(s) 10, 36, 39
HpyAV CCTTC 5 cut(s) 127, 229, 617, 697, 988
HpyCH4III ACNGT 2 cut(s) 641, 810
HpyCH4IV ACGT 2 cut(s) 5, 34
HpyCH4V TGCA 4 cut(s) 635, 752, 930, 950
HpyF10VI GCNNNNNNNGC 3 cut(s) 82, 848, 956
HpyF3I CTNAG 7 cut(s) 129, 203, 455, 489, 500, 530, 894
HpySE526I ACGT 2 cut(s) 5, 34
Hsp92I GRCGYC 3 cut(s) 5, 24, 34
Hsp92II CATG 7 cut(s) 346, 395, 664, 839, 860, 983, 994
HspAI GCGC 2 cut(s) 102, 1038
Ksp22I TGATCA 1 cut(s) 691
Kzo9I GATC 6 cut(s) 367, 397, 444, 691, 745, 913
LguI GCTCTTC 2 cut(s) 357, 500
LmnI GCTCC 5 cut(s) 44, 82, 125, 451, 908
Lsp1109I GCAGC 2 cut(s) 459, 826
LweI GCATC 2 cut(s) 519, 586
MaeI CTAG 3 cut(s) 252, 434, 773
MaeII ACGT 2 cut(s) 5, 34
MalI GATC 6 cut(s) 369, 399, 446, 693, 747, 915
MboI GATC 6 cut(s) 367, 397, 444, 691, 745, 913
MboII GAAGA 6 cut(s) 73, 164, 344, 407, 487, 995
MflI RGATCY 4 cut(s) 397, 444, 745, 913
MhlI GDGCHC 4 cut(s) 87, 308, 352, 495
MluCI AATT 5 cut(s) 578, 822, 831, 864, 1007
MlyI GAGTC 2 cut(s) 83, 105
MmeI TCCRAC 1 cut(s) 784
MnlI CCTC 9 cut(s) 13, 116, 219, 339, 355, 422, 486, 868, 889
MseI TTAA 3 cut(s) 672, 828, 903
MslI CAYNNNNRTG 1 cut(s) 1047
MspA1I CMGCKG 2 cut(s) 302, 422
MspI CCGG 4 cut(s) 58, 402, 448, 778
MspR9I CCNGG 4 cut(s) 402, 449, 587, 765
MvaI CCWGG 2 cut(s) 587, 765
MvnI CGCG 2 cut(s) 102, 966
MwoI GCNNNNNNNGC 3 cut(s) 82, 848, 956
NciI CCSGG 2 cut(s) 402, 449
NdeII GATC 6 cut(s) 367, 397, 444, 691, 745, 913
NlaIII CATG 7 cut(s) 346, 395, 664, 839, 860, 983, 994
NlaIV GGNNCC 1 cut(s) 628
PagI TCATGA 4 cut(s) 342, 391, 979, 990
PciSI GCTCTTC 2 cut(s) 357, 500
PfeI GAWTC 4 cut(s) 461, 534, 790, 1031
PfoI TCCNGGA 1 cut(s) 447
PkrI GCNGC 2 cut(s) 474, 841
PleI GAGTC 2 cut(s) 83, 104
PpsI GAGTC 2 cut(s) 83, 104
Psp124BI GAGCTC 2 cut(s) 87, 495
Psp6I CCWGG 2 cut(s) 585, 763
PspFI CCCAGC 1 cut(s) 938
PspGI CCWGG 2 cut(s) 585, 763
PspN4I GGNNCC 1 cut(s) 628
PspPI GGNCC 2 cut(s) 248, 627
PsuI RGATCY 4 cut(s) 397, 444, 745, 913
RseI CAYNNNNRTG 1 cut(s) 1047
SacI GAGCTC 2 cut(s) 87, 495
SalI GTCGAC 1 cut(s) 7
SapI GCTCTTC 2 cut(s) 357, 500
SaqAI TTAA 3 cut(s) 672, 828, 903
SatI GCNGC 2 cut(s) 473, 840
Sau3AI GATC 6 cut(s) 367, 397, 444, 691, 745, 913
Sau96I GGNCC 2 cut(s) 248, 627
SchI GAGTC 2 cut(s) 83, 105
ScrFI CCNGG 4 cut(s) 402, 449, 587, 765
SduI GDGCHC 4 cut(s) 87, 308, 352, 495
SfaNI GCATC 2 cut(s) 519, 586
SfcI CTRYAG 1 cut(s) 465
SinI GGWCC 1 cut(s) 627
SmiMI CAYNNNNRTG 1 cut(s) 1047
Sse9I AATT 5 cut(s) 578, 822, 831, 864, 1007
SsiI CCGC 6 cut(s) 42, 100, 109, 302, 420, 919
SspI AATATT 1 cut(s) 559
SspMI CTAG 3 cut(s) 252, 434, 773
SstI GAGCTC 2 cut(s) 87, 495
StyD4I CCNGG 4 cut(s) 400, 447, 585, 763
StyI CCWWGG 1 cut(s) 251
TaaI ACNGT 2 cut(s) 641, 810
TaiI ACGT 2 cut(s) 8, 37
TaqI TCGA 5 cut(s) 8, 87, 213, 594, 1034
TaqII GACCGA 1 cut(s) 26
TasI AATT 5 cut(s) 578, 822, 831, 864, 1007
TfiI GAWTC 4 cut(s) 461, 534, 790, 1031
Tru1I TTAA 3 cut(s) 672, 828, 903
Tru9I TTAA 3 cut(s) 672, 828, 903
TscAI CASTG 4 cut(s) 55, 111, 637, 937
TseI GCWGC 2 cut(s) 472, 839
TspGWI ACGGA 1 cut(s) 944
TspRI CASTG 4 cut(s) 55, 111, 637, 937
VpaK11BI GGWCC 1 cut(s) 627
XapI RAATTY 2 cut(s) 822, 831
XmaJI CCTAGG 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 8
XspI CTAG 3 cut(s) 252, 434, 773
ZraI GACGTC 2 cut(s) 6, 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.