MD05G1060900.v1.1

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004350
Physical Location & Seq
Forward (+)
10577822 .. 10581304
3483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1060900.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGACGACTGAGACAGAAGACGCCGTACGACGTCGCACAGCGGTTGCCGACTACCGCAAAAGGTTACTCCAGCACAAGGAGCTGAAATCCCGAGTCCGCGCAGTGAGAGAGAACTTGCGGGCTGCGAAGAAAGAATTTGGGAAAACTGAAGATGATTTGAAGTCCCTTCAAAGTGTTGGACAGATTATTGGTGAAGTTCTCAGGCCTCTTGATAACGAACGCCTAATTGTGAAGGCAAGTAGTGGCCCGAGGTACGTCGTTGGGTGTCGCAGTAAAGTTGACAAAGAAAAACTAACCGCTGGAACGCGTGTTGTTTTGGATATGACTACCTTGACTATCATGCGGGCTCTACCCAGAGAAGTTGATCCGGTTGTATATAACATGCTTCATGAAGATCCTGGTAATGTTAGCTACTCAGCCGTCGGAGGACTATCTGATCAAATCCGAGAGCTTAGGGAGTCTATTGAACTGCCTCTAATGAACCCTGAGCTCTTCCTTAGGGTGGGGATCAAACCTCCCAAGGGTGTTCTTCTGTATGGACCTCCGGGAACAGGGAAGACATTGCTAGCCAGAGCAATTGCTAGCAACATAGATGCTAACTTTCTTAAGGTTGTATCAAGTGCCATTATTGACAAATATATCGGTGAAAGTGCAAGATTGATAAGGGAAATGTTTGGTTATGCCCGTGATCACCAGCCATGTATCATTTTTATGGATGAGATCGATGCTATTGGAGGACGGCGTTTTAGTGAAGGGACTAGTGCAGACCGAGAAATTCAGCGAACACTCATGGAGTTGCTTAATCAGTTAGATGGGTTTGATCAGCTCGGAAAGGTGAAAATGATCATGGCAACCAATAGGCCTGATGTACTGGATCCAGCACTTCTCCGCCCTGGGCGACTAGACCGCAAGATAGAGATCCCATTGCCCAATGAGCAATCAAGAATGGAAATTCTCAAAATCCATGCTGCTGGGATAGCCAAACATGGGGACATTGATTATGAGGCAGTTGTGAAGCTTGCTGAGGGTTTTAATGGAGCTGATCTCCGTAATGTCTGCACTGAAGCTGGGATGTCTGCGATCCGTGCTGAGAGGGATTATGTCATCCATGAAGATTTCATGAAGGCTGTACGGAAACTGAATGAGGCCAAGAAACTCGAATCTAGTGCCCACTACAACACAGATTTTGGGAAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005838 GO:0005886 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0022624 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

400

Amino Acids

44.69

Weight (kDa)

8.74

Isoelectric Point (pI)

35.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prot_ATP_ID_OB_C PF16450 63 - 118 1e-14 Proteasomal ATPase OB C-terminal domain
RuvB_N PF05496 175 - 242 8.6e-06 Holliday junction DNA helicase RuvB P-loop domain
AAA PF00004 176 - 309 1.2e-43 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 331 - 374 4.5e-13 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 34
AccII CGCG 2 cut(s) 99, 307
AciI CCGC 8 cut(s) 41, 55, 97, 118, 297, 343, 889, 907
AclWI GGATC 7 cut(s) 359, 389, 515, 869, 882, 913, 1075
AcsI RAATTY 3 cut(s) 134, 774, 951
AcuI CTGAAG 2 cut(s) 168, 1083
AcyI GRCGYC 2 cut(s) 21, 31
AfaI GTAC 4 cut(s) 27, 254, 870, 1131
AfiI CCNNNNNNNGG 5 cut(s) 76, 502, 521, 551, 987
AflII CTTAAG 1 cut(s) 605
AflIII ACRYGT 1 cut(s) 305
AgsI TTSAA 3 cut(s) 160, 170, 467
AhlI ACTAGT 1 cut(s) 758
AjnI CCWGG 2 cut(s) 397, 892
AluBI AGCT 8 cut(s) 82, 411, 451, 490, 826, 1018, 1040, 1067
AluI AGCT 8 cut(s) 82, 411, 451, 490, 826, 1018, 1040, 1067
Alw21I GWGCWC 1 cut(s) 492
Alw26I GTCTC 1 cut(s) 5
AlwI GGATC 7 cut(s) 359, 389, 515, 869, 882, 913, 1075
Ama87I CYCGRG 2 cut(s) 90, 247
AoxI GGCC 4 cut(s) 203, 244, 860, 1146
ApeKI GCWGC 2 cut(s) 122, 968
ApoI RAATTY 3 cut(s) 134, 774, 951
AspLEI GCGC 1 cut(s) 101
AspS9I GGNCC 2 cut(s) 245, 539
AsuC2I CCSGG 1 cut(s) 546
AsuHPI GGTGA 4 cut(s) 203, 656, 683, 847
AsuNHI GCTAGC 2 cut(s) 565, 581
AvaI CYCGRG 2 cut(s) 90, 247
AvaII GGWCC 1 cut(s) 539
AxyI CCTNAGG 1 cut(s) 497
BaeGI GKGCMC 1 cut(s) 1171
BamHI GGATCC 1 cut(s) 874
BanII GRGCYC 2 cut(s) 349, 492
BarI GAAGNNNNNNTAC 2 cut(s) 9, 41
BbsI GAAGAC 2 cut(s) 24, 563
Bbv12I GWGCWC 1 cut(s) 492
BbvCI CCTCAGC 1 cut(s) 1023
BbvI GCAGC 2 cut(s) 109, 955
BccI CCATC 1 cut(s) 806
BceAI ACGGC 3 cut(s) 8, 404, 755
BcgI CGANNNNNNTGC 2 cut(s) 1148, 1182
BciT130I CCWGG 2 cut(s) 399, 894
BclI TGATCA 4 cut(s) 436, 688, 820, 843
BcnI CCSGG 1 cut(s) 546
BcoDI GTCTC 1 cut(s) 5
BcuI ACTAGT 1 cut(s) 758
BfaI CTAG 5 cut(s) 566, 582, 759, 902, 1164
BfrI CTTAAG 1 cut(s) 605
BisI GCNGC 2 cut(s) 123, 969
BlsI GCNGC 2 cut(s) 124, 970
Bme1390I CCNGG 3 cut(s) 399, 546, 894
Bme18I GGWCC 1 cut(s) 539
BmeT110I CYCGRG 2 cut(s) 90, 247
BmgT120I GGNCC 2 cut(s) 245, 539
BmiI GGNNCC 1 cut(s) 876
BmrFI CCNGG 3 cut(s) 399, 546, 894
BmsI GCATC 2 cut(s) 583, 715
BmtI GCTAGC 2 cut(s) 569, 585
BpiI GAAGAC 2 cut(s) 24, 563
BplI GAGNNNNNCTC 2 cut(s) 1029, 1061
BpmI CTGGAG 1 cut(s) 53
Bpu10I CCTNAGC 3 cut(s) 452, 486, 1023
BpuMI CCSGG 1 cut(s) 546
Bsa29I ATCGAT 1 cut(s) 723
BsaBI GATNNNNATC 1 cut(s) 917
BsaHI GRCGYC 2 cut(s) 21, 31
BsaJI CCNNGG 4 cut(s) 248, 519, 892, 893
BsaWI WCCGGW 1 cut(s) 367
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 502, 521, 551, 987
Bse1I ACTGG 1 cut(s) 876
Bse21I CCTNAGG 1 cut(s) 497
Bse3DI GCAATG 2 cut(s) 560, 923
Bse8I GATNNNNATC 1 cut(s) 917
BseBI CCWGG 2 cut(s) 399, 894
BseCI ATCGAT 1 cut(s) 723
BseDI CCNNGG 4 cut(s) 248, 519, 892, 893
BseGI GGATG 3 cut(s) 721, 1077, 1104
BseJI GATNNNNATC 1 cut(s) 917
BseLI CCNNNNNNNGG 5 cut(s) 76, 502, 521, 551, 987
BseMI GCAATG 2 cut(s) 560, 923
BseMII CTCAG 5 cut(s) 214, 429, 477, 1014, 1080
BseNI ACTGG 1 cut(s) 876
BseSI GKGCMC 1 cut(s) 1171
BseXI GCAGC 2 cut(s) 109, 955
BseYI CCCAGC 2 cut(s) 971, 1067
BsgI GTGCAG 2 cut(s) 783, 1042
Bsh1236I CGCG 2 cut(s) 99, 307
BshFI GGCC 4 cut(s) 205, 246, 862, 1148
BshVI ATCGAT 1 cut(s) 723
BsiHKAI GWGCWC 1 cut(s) 492
BsiHKCI CYCGRG 2 cut(s) 90, 247
BsiSI CCGG 2 cut(s) 368, 545
BsiWI CGTACG 1 cut(s) 25
BslFI GGGAC 3 cut(s) 148, 769, 1004
BslI CCNNNNNNNGG 5 cut(s) 76, 502, 521, 551, 987
BsmAI GTCTC 1 cut(s) 5
BsmFI GGGAC 3 cut(s) 148, 769, 1004
BsnI GGCC 4 cut(s) 205, 246, 862, 1148
BsoBI CYCGRG 2 cut(s) 90, 247
Bsp1286I GDGCHC 3 cut(s) 349, 492, 1171
BspACI CCGC 8 cut(s) 41, 55, 97, 118, 297, 343, 889, 907
BspANI GGCC 4 cut(s) 205, 246, 862, 1148
BspCNI CTCAG 5 cut(s) 213, 428, 478, 1015, 1081
BspDI ATCGAT 1 cut(s) 723
BspFNI CGCG 2 cut(s) 99, 307
BspHI TCATGA 2 cut(s) 388, 1119
BspLI GGNNCC 1 cut(s) 876
BspOI GCTAGC 2 cut(s) 569, 585
BspPI GGATC 7 cut(s) 359, 389, 515, 869, 882, 913, 1075
BspQI GCTCTTC 1 cut(s) 497
BspTI CTTAAG 1 cut(s) 605
BsrDI GCAATG 2 cut(s) 560, 923
BsrI ACTGG 1 cut(s) 876
BssECI CCNNGG 4 cut(s) 248, 519, 892, 893
BssNI GRCGYC 2 cut(s) 21, 31
BssT1I CCWWGG 1 cut(s) 519
Bst2UI CCWGG 2 cut(s) 399, 894
Bst6I CTCTTC 1 cut(s) 497
BstACI GRCGYC 2 cut(s) 21, 31
BstAFI CTTAAG 1 cut(s) 605
BstC8I GCNNGC 5 cut(s) 120, 345, 567, 583, 1020
BstDEI CTNAG 8 cut(s) 9, 200, 415, 452, 486, 497, 1023, 1089
BstF5I GGATG 3 cut(s) 721, 1077, 1104
BstFNI CGCG 2 cut(s) 99, 307
BstHHI GCGC 1 cut(s) 101
BstMAI GTCTC 1 cut(s) 5
BstMWI GCNNNNNNNGC 4 cut(s) 79, 934, 977, 1085
BstNI CCWGG 2 cut(s) 399, 894
BstNSI RCATGY 1 cut(s) 385
BstSCI CCNGG 3 cut(s) 397, 544, 892
BstSLI GKGCMC 1 cut(s) 1171
BstUI CGCG 2 cut(s) 99, 307
BstV1I GCAGC 2 cut(s) 109, 955
BstV2I GAAGAC 2 cut(s) 24, 563
BstX2I RGATCY 3 cut(s) 394, 874, 918
BstXI CCANNNNNNTGG 1 cut(s) 971
BstYI RGATCY 3 cut(s) 394, 874, 918
Bsu15I ATCGAT 1 cut(s) 723
Bsu36I CCTNAGG 1 cut(s) 497
BsuRI GGCC 4 cut(s) 205, 246, 862, 1148
BsuTUI ATCGAT 1 cut(s) 723
BtsCI GGATG 3 cut(s) 721, 1077, 1104
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 2 cut(s) 108, 1059
Cac8I GCNNGC 5 cut(s) 120, 345, 567, 583, 1020
CciI TCATGA 2 cut(s) 388, 1119
CfoI GCGC 1 cut(s) 101
Cfr13I GGNCC 2 cut(s) 245, 539
ClaI ATCGAT 1 cut(s) 723
CseI GACGC 1 cut(s) 29
Csp6I GTAC 4 cut(s) 26, 253, 869, 1130
CviQI GTAC 4 cut(s) 26, 253, 869, 1130
DdeI CTNAG 8 cut(s) 9, 200, 415, 452, 486, 497, 1023, 1089
Eam1104I CTCTTC 1 cut(s) 497
EarI CTCTTC 1 cut(s) 497
EciI GGCGGA 1 cut(s) 878
Ecl136II GAGCTC 1 cut(s) 490
Eco130I CCWWGG 1 cut(s) 519
Eco147I AGGCCT 2 cut(s) 205, 862
Eco24I GRGCYC 2 cut(s) 349, 492
Eco47I GGWCC 1 cut(s) 539
Eco53kI GAGCTC 1 cut(s) 490
Eco57I CTGAAG 2 cut(s) 168, 1083
Eco81I CCTNAGG 1 cut(s) 497
Eco88I CYCGRG 2 cut(s) 90, 247
EcoICRI GAGCTC 1 cut(s) 490
EcoRII CCWGG 2 cut(s) 397, 892
EcoT14I CCWWGG 1 cut(s) 519
EcoT38I GRGCYC 2 cut(s) 349, 492
ErhI CCWWGG 1 cut(s) 519
FaqI GGGAC 3 cut(s) 148, 769, 1004
FauI CCCGC 2 cut(s) 111, 336
FbaI TGATCA 4 cut(s) 436, 688, 820, 843
Fnu4HI GCNGC 2 cut(s) 123, 969
FokI GGATG 3 cut(s) 728, 1084, 1091
FriOI GRGCYC 2 cut(s) 349, 492
Fsp4HI GCNGC 2 cut(s) 123, 969
FspBI CTAG 5 cut(s) 566, 582, 759, 902, 1164
GlaI GCGC 1 cut(s) 100
GluI GCNGC 2 cut(s) 123, 969
GsaI CCCAGC 2 cut(s) 975, 1071
GsuI CTGGAG 1 cut(s) 53
HaeIII GGCC 4 cut(s) 205, 246, 862, 1148
HapII CCGG 2 cut(s) 368, 545
HgaI GACGC 1 cut(s) 29
HhaI GCGC 1 cut(s) 101
Hin1I GRCGYC 2 cut(s) 21, 31
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 1 cut(s) 1016
HinfI GANTC 3 cut(s) 93, 458, 1160
HpaII CCGG 2 cut(s) 368, 545
HphI GGTGA 4 cut(s) 203, 656, 683, 847
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 4 cut(s) 425, 436, 446, 830
Hpy188III TCNNGA 5 cut(s) 90, 209, 389, 942, 1120
Hpy8I GTNNAC 1 cut(s) 280
Hpy99I CGWCG 4 cut(s) 33, 36, 260, 425
HpyAV CCTTC 4 cut(s) 176, 226, 746, 1117
HpyCH4IV ACGT 2 cut(s) 31, 255
HpyCH4V TGCA 3 cut(s) 653, 764, 1059
HpyF10VI GCNNNNNNNGC 4 cut(s) 79, 934, 977, 1085
HpyF3I CTNAG 8 cut(s) 9, 200, 415, 452, 486, 497, 1023, 1089
HpySE526I ACGT 2 cut(s) 31, 255
Hsp92I GRCGYC 2 cut(s) 21, 31
HspAI GCGC 1 cut(s) 99
Ksp22I TGATCA 4 cut(s) 436, 688, 820, 843
LguI GCTCTTC 1 cut(s) 497
LmnI GCTCC 2 cut(s) 79, 1037
Lsp1109I GCAGC 2 cut(s) 109, 955
LweI GCATC 2 cut(s) 583, 715
MaeI CTAG 5 cut(s) 566, 582, 759, 902, 1164
MaeII ACGT 2 cut(s) 31, 255
MaeIII GTNAC 1 cut(s) 63
MboII GAAGA 8 cut(s) 29, 139, 161, 404, 484, 521, 568, 1124
MfeI CAATTG 1 cut(s) 576
MflI RGATCY 3 cut(s) 394, 874, 918
MhlI GDGCHC 3 cut(s) 349, 492, 1171
MluCI AATT 5 cut(s) 134, 225, 576, 774, 951
MluI ACGCGT 1 cut(s) 305
MlyI GAGTC 2 cut(s) 102, 467
MmeI TCCRAC 2 cut(s) 157, 403
MseI TTAA 3 cut(s) 606, 801, 1032
MslI CAYNNNNRTG 1 cut(s) 710
MspA1I CMGCKG 2 cut(s) 41, 299
MspCI CTTAAG 1 cut(s) 605
MspI CCGG 2 cut(s) 368, 545
MspR9I CCNGG 3 cut(s) 399, 546, 894
MunI CAATTG 1 cut(s) 576
MvaI CCWGG 2 cut(s) 399, 894
MvnI CGCG 2 cut(s) 99, 307
MwoI GCNNNNNNNGC 4 cut(s) 79, 934, 977, 1085
NciI CCSGG 1 cut(s) 546
NheI GCTAGC 2 cut(s) 565, 581
NlaIV GGNNCC 1 cut(s) 876
NspI RCATGY 1 cut(s) 385
PagI TCATGA 2 cut(s) 388, 1119
PasI CCCWGGG 1 cut(s) 893
PceI AGGCCT 2 cut(s) 205, 862
PciSI GCTCTTC 1 cut(s) 497
PfeI GAWTC 1 cut(s) 1160
Pfl23II CGTACG 1 cut(s) 25
PfoI TCCNGGA 1 cut(s) 544
PkrI GCNGC 2 cut(s) 124, 970
PleI GAGTC 2 cut(s) 101, 466
PpsI GAGTC 2 cut(s) 101, 466
Psp124BI GAGCTC 1 cut(s) 492
Psp6I CCWGG 2 cut(s) 397, 892
PspFI CCCAGC 2 cut(s) 971, 1067
PspGI CCWGG 2 cut(s) 397, 892
PspLI CGTACG 1 cut(s) 25
PspN4I GGNNCC 1 cut(s) 876
PspPI GGNCC 2 cut(s) 245, 539
PsuI RGATCY 3 cut(s) 394, 874, 918
RsaI GTAC 4 cut(s) 27, 254, 870, 1131
RsaNI GTAC 4 cut(s) 26, 253, 869, 1130
RseI CAYNNNNRTG 1 cut(s) 710
SacI GAGCTC 1 cut(s) 492
SapI GCTCTTC 1 cut(s) 497
SaqAI TTAA 3 cut(s) 606, 801, 1032
SatI GCNGC 2 cut(s) 123, 969
Sau96I GGNCC 2 cut(s) 245, 539
SchI GAGTC 2 cut(s) 102, 467
ScrFI CCNGG 3 cut(s) 399, 546, 894
SduI GDGCHC 3 cut(s) 349, 492, 1171
SfaNI GCATC 2 cut(s) 583, 715
SinI GGWCC 1 cut(s) 539
SmiMI CAYNNNNRTG 1 cut(s) 710
SmlI CTYRAG 1 cut(s) 605
SmoI CTYRAG 1 cut(s) 605
SpeI ACTAGT 1 cut(s) 758
Sse9I AATT 5 cut(s) 134, 225, 576, 774, 951
SseBI AGGCCT 2 cut(s) 205, 862
SsiI CCGC 8 cut(s) 41, 55, 97, 118, 297, 343, 889, 907
SspMI CTAG 5 cut(s) 566, 582, 759, 902, 1164
SstI GAGCTC 1 cut(s) 492
StuI AGGCCT 2 cut(s) 205, 862
StyD4I CCNGG 3 cut(s) 397, 544, 892
StyI CCWWGG 1 cut(s) 519
TaiI ACGT 2 cut(s) 34, 258
TaqI TCGA 2 cut(s) 723, 1158
TaqII GACCGA 1 cut(s) 783
TasI AATT 5 cut(s) 134, 225, 576, 774, 951
TatI WGTACW 1 cut(s) 868
TfiI GAWTC 1 cut(s) 1160
Tru1I TTAA 3 cut(s) 606, 801, 1032
Tru9I TTAA 3 cut(s) 606, 801, 1032
TscAI CASTG 2 cut(s) 108, 1066
TseI GCWGC 2 cut(s) 122, 968
TspDTI ATGAA 6 cut(s) 377, 405, 494, 1108, 1125, 1136
TspGWI ACGGA 3 cut(s) 1037, 1073, 1147
TspRI CASTG 2 cut(s) 108, 1066
Vha464I CTTAAG 1 cut(s) 605
VpaK11BI GGWCC 1 cut(s) 539
XapI RAATTY 3 cut(s) 134, 774, 951
XceI RCATGY 1 cut(s) 385
XspI CTAG 5 cut(s) 566, 582, 759, 902, 1164
ZraI GACGTC 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.