MD04G1152800.v1.1

Protein TWIN LOV

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011750
Physical Location & Seq
Reverse (-)
24151421 .. 24152048
628 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1152800.v1.1.491

Sequence Viewer

Length: 522 bp
ATGGAATCAGAGCTGGGTTTAATCGAGCAGTCGTTCATCAGCTGCTACTTGCTCTGGGTCCAGGAAGCTCTGGGGGAATTGCCCGATAGCTTCACGATCACTGACCCTTGGATTTCTGGGCACCCAATCGTGTTCGCGAGCAAAGAGTTCCTGAAAATGTCGGGATATTCGAAGAACGAGGTGGTCGGGAGGAACAACAGAGTGTTTCAAGGTCCGAGGACTTGCAGGCGGTCGTTCATGGAGGTCCGGAAAGCCATTAGGGAGGAGCGAGCTGTTCTAGTTAATTTGCTGAATTCTCGGAAAGGCGGGACGCCCTTTTTGATGATGTTTCATTTGTGTCACAAGTTTGCTCATGCCACATTGAAAGGTCCAAGTGTTGTCAAAGAGATATTGTATGGATCAGTGCTTGTTTTGGCTTGTGGAAGTCTGTGGAAAATGCATCACTGGAATGAGCAGAGGAAAGTGAGGGCGTTCTATGACTTGCTCGAGAAGGGTGAAATCAGTGTTGTCGTTGAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS_9

Protein Analysis

174

Amino Acids

19.85

Weight (kDa)

8.69

Isoelectric Point (pI)

55.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_9 PF13426 40 - 113 2.2e-08 PAS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 120
AccII CGCG 1 cut(s) 137
AccIII TCCGGA 1 cut(s) 246
AciI CCGC 2 cut(s) 229, 306
AclWI GGATC 1 cut(s) 406
AcsI RAATTY 1 cut(s) 292
AcyI GRCGYC 1 cut(s) 311
AgsI TTSAA 3 cut(s) 209, 364, 515
AjnI CCWGG 1 cut(s) 60
AluBI AGCT 5 cut(s) 13, 42, 68, 90, 272
AluI AGCT 5 cut(s) 13, 42, 68, 90, 272
AlwI GGATC 1 cut(s) 406
Ama87I CYCGRG 1 cut(s) 485
Aor13HI TCCGGA 1 cut(s) 246
ApeKI GCWGC 1 cut(s) 42
ApoI RAATTY 1 cut(s) 292
ArsI GACNNNNNNTTYG 2 cut(s) 301, 333
AspS9I GGNCC 4 cut(s) 58, 212, 244, 368
AsuHPI GGTGA 1 cut(s) 506
AsuII TTCGAA 1 cut(s) 170
AvaI CYCGRG 1 cut(s) 485
AvaII GGWCC 4 cut(s) 58, 212, 244, 368
BaeGI GKGCMC 1 cut(s) 123
BanI GGYRCC 1 cut(s) 120
BbvI GCAGC 1 cut(s) 29
BciT130I CCWGG 1 cut(s) 62
BfaI CTAG 1 cut(s) 278
BisI GCNGC 1 cut(s) 43
BlsI GCNGC 1 cut(s) 44
Bme1390I CCNGG 1 cut(s) 62
Bme18I GGWCC 4 cut(s) 58, 212, 244, 368
BmeT110I CYCGRG 1 cut(s) 485
BmgT120I GGNCC 4 cut(s) 58, 212, 244, 368
BmiI GGNNCC 2 cut(s) 59, 122
BmrFI CCNGG 1 cut(s) 62
BmsI GCATC 1 cut(s) 448
Bpu14I TTCGAA 1 cut(s) 170
BsaHI GRCGYC 1 cut(s) 311
BsaJI CCNNGG 2 cut(s) 107, 215
BsaWI WCCGGW 1 cut(s) 246
Bse1I ACTGG 1 cut(s) 449
BseAI TCCGGA 1 cut(s) 246
BseBI CCWGG 1 cut(s) 62
BseDI CCNNGG 2 cut(s) 107, 215
BseNI ACTGG 1 cut(s) 449
BseRI GAGGAG 1 cut(s) 278
BseSI GKGCMC 1 cut(s) 123
BseXI GCAGC 1 cut(s) 29
BseYI CCCAGC 1 cut(s) 13
Bsh1236I CGCG 1 cut(s) 137
Bsh1285I CGRYCG 1 cut(s) 233
BshNI GGYRCC 1 cut(s) 120
BsiEI CGRYCG 1 cut(s) 233
BsiHKCI CYCGRG 1 cut(s) 485
BsiSI CCGG 1 cut(s) 247
BslFI GGGAC 1 cut(s) 322
BsmFI GGGAC 1 cut(s) 322
BsoBI CYCGRG 1 cut(s) 485
Bsp119I TTCGAA 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 123
Bsp13I TCCGGA 1 cut(s) 246
Bsp143I GATC 2 cut(s) 96, 398
Bsp68I TCGCGA 1 cut(s) 137
BspACI CCGC 2 cut(s) 229, 306
BspEI TCCGGA 1 cut(s) 246
BspFNI CGCG 1 cut(s) 137
BspLI GGNNCC 2 cut(s) 59, 122
BspPI GGATC 1 cut(s) 406
BspT104I TTCGAA 1 cut(s) 170
BspT107I GGYRCC 1 cut(s) 120
BsrI ACTGG 1 cut(s) 449
BssECI CCNNGG 2 cut(s) 107, 215
BssMI GATC 2 cut(s) 96, 398
BssNI GRCGYC 1 cut(s) 311
BssT1I CCWWGG 1 cut(s) 107
Bst2UI CCWGG 1 cut(s) 62
BstACI GRCGYC 1 cut(s) 311
BstBI TTCGAA 1 cut(s) 170
BstC8I GCNNGC 3 cut(s) 139, 227, 270
BstFNI CGCG 1 cut(s) 137
BstKTI GATC 2 cut(s) 99, 401
BstMBI GATC 2 cut(s) 96, 398
BstMCI CGRYCG 1 cut(s) 233
BstNI CCWGG 1 cut(s) 62
BstSCI CCNGG 1 cut(s) 60
BstSLI GKGCMC 1 cut(s) 123
BstUI CGCG 1 cut(s) 137
BstV1I GCAGC 1 cut(s) 29
BtsIMutI CAGTG 4 cut(s) 99, 408, 442, 508
BtuMI TCGCGA 1 cut(s) 137
Cac8I GCNNGC 3 cut(s) 139, 227, 270
Cfr13I GGNCC 4 cut(s) 58, 212, 244, 368
CseI GACGC 1 cut(s) 319
CviAII CATG 2 cut(s) 238, 353
CviJI RGCY 7 cut(s) 13, 42, 68, 90, 254, 272, 416
CviKI_1 RGCY 7 cut(s) 13, 42, 68, 90, 254, 272, 416
DpnI GATC 2 cut(s) 98, 400
DpnII GATC 2 cut(s) 96, 398
Eco130I CCWWGG 1 cut(s) 107
Eco47I GGWCC 4 cut(s) 58, 212, 244, 368
Eco88I CYCGRG 1 cut(s) 485
EcoRI GAATTC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 60
EcoT14I CCWWGG 1 cut(s) 107
EcoT22I ATGCAT 1 cut(s) 441
ErhI CCWWGG 1 cut(s) 107
FaeI CATG 2 cut(s) 241, 356
FaiI YATR 4 cut(s) 239, 354, 396, 477
FaqI GGGAC 1 cut(s) 322
FatI CATG 2 cut(s) 237, 352
FauI CCCGC 1 cut(s) 299
Fnu4HI GCNGC 1 cut(s) 43
Fsp4HI GCNGC 1 cut(s) 43
FspBI CTAG 1 cut(s) 278
GluI GCNGC 1 cut(s) 43
GsaI CCCAGC 1 cut(s) 17
HapII CCGG 1 cut(s) 247
HgaI GACGC 1 cut(s) 319
Hin1I GRCGYC 1 cut(s) 311
Hin1II CATG 2 cut(s) 241, 356
HinfI GANTC 1 cut(s) 5
HpaII CCGG 1 cut(s) 247
HphI GGTGA 1 cut(s) 506
Hpy188I TCNGA 3 cut(s) 10, 216, 300
Hpy188III TCNNGA 7 cut(s) 94, 136, 151, 162, 187, 247, 487
HpyAV CCTTC 1 cut(s) 484
HpyCH4V TGCA 2 cut(s) 225, 439
Hsp92I GRCGYC 1 cut(s) 311
Hsp92II CATG 2 cut(s) 241, 356
Kpn2I TCCGGA 1 cut(s) 246
Kzo9I GATC 2 cut(s) 96, 398
LmnI GCTCC 1 cut(s) 265
LpnPI CCDG 9 cut(s) 40, 47, 56, 74, 102, 164, 211, 260, 430
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 1 cut(s) 448
MaeI CTAG 1 cut(s) 278
MaeIII GTNAC 1 cut(s) 338
MalI GATC 2 cut(s) 98, 400
MboI GATC 2 cut(s) 96, 398
MboII GAAGA 1 cut(s) 184
MhlI GDGCHC 1 cut(s) 123
MluCI AATT 3 cut(s) 77, 283, 292
MnlI CCTC 7 cut(s) 172, 183, 210, 235, 256, 450, 459
Mph1103I ATGCAT 1 cut(s) 441
MroI TCCGGA 1 cut(s) 246
MseI TTAA 2 cut(s) 20, 282
MslI CAYNNNNRTG 1 cut(s) 447
MspA1I CMGCKG 1 cut(s) 42
MspI CCGG 1 cut(s) 247
MspR9I CCNGG 1 cut(s) 62
MvaI CCWGG 1 cut(s) 62
MvnI CGCG 1 cut(s) 137
NdeII GATC 2 cut(s) 96, 398
NlaIII CATG 2 cut(s) 241, 356
NlaIV GGNNCC 2 cut(s) 59, 122
NmuCI GTSAC 1 cut(s) 338
NruI TCGCGA 1 cut(s) 137
NsiI ATGCAT 1 cut(s) 441
NspV TTCGAA 1 cut(s) 170
PaeR7I CTCGAG 1 cut(s) 485
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PfeI GAWTC 1 cut(s) 5
PfoI TCCNGGA 1 cut(s) 60
PkrI GCNGC 1 cut(s) 44
Psp6I CCWGG 1 cut(s) 60
PspFI CCCAGC 1 cut(s) 13
PspGI CCWGG 1 cut(s) 60
PspN4I GGNNCC 2 cut(s) 59, 122
PspPI GGNCC 4 cut(s) 58, 212, 244, 368
PvuII CAGCTG 1 cut(s) 42
RruI TCGCGA 1 cut(s) 137
RseI CAYNNNNRTG 1 cut(s) 447
SaqAI TTAA 2 cut(s) 20, 282
SatI GCNGC 1 cut(s) 43
Sau3AI GATC 2 cut(s) 96, 398
Sau96I GGNCC 4 cut(s) 58, 212, 244, 368
ScrFI CCNGG 1 cut(s) 62
SduI GDGCHC 1 cut(s) 123
SetI ASST 9 cut(s) 15, 44, 70, 92, 183, 214, 246, 274, 370
SfaNI GCATC 1 cut(s) 448
Sfr274I CTCGAG 1 cut(s) 485
SfuI TTCGAA 1 cut(s) 170
SinI GGWCC 4 cut(s) 58, 212, 244, 368
SlaI CTCGAG 1 cut(s) 485
SmiMI CAYNNNNRTG 1 cut(s) 447
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 3 cut(s) 77, 283, 292
SsiI CCGC 2 cut(s) 229, 306
SspMI CTAG 1 cut(s) 278
StyD4I CCNGG 1 cut(s) 60
StyI CCWWGG 1 cut(s) 107
TaqI TCGA 3 cut(s) 24, 170, 486
TasI AATT 3 cut(s) 77, 283, 292
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 2 cut(s) 20, 282
Tru9I TTAA 2 cut(s) 20, 282
TscAI CASTG 4 cut(s) 106, 408, 449, 508
TseFI GTSAC 1 cut(s) 338
TseI GCWGC 1 cut(s) 42
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 3 cut(s) 25, 226, 320
TspRI CASTG 4 cut(s) 106, 408, 449, 508
VpaK11BI GGWCC 4 cut(s) 58, 212, 244, 368
XapI RAATTY 1 cut(s) 292
XhoI CTCGAG 1 cut(s) 485
XspI CTAG 1 cut(s) 278
Zsp2I ATGCAT 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.