MD04G1167600.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012275
Physical Location & Seq
Forward (+)
25790290 .. 25791991
1702 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1167600.v1.1.491

Sequence Viewer

Length: 633 bp
ATGAAGAATCAAGACGATGCACCACCGAAATTCGATGAACAAGAGGTCGGATCACCGCCCCCGGTTGTCCCAACGCGCCCACCGGCCGCTGACGTGGAGAATCAGACCCCGTCCCAGATCAGAACTGCTGGGACGGGGTTTGGGGTCGCGGGGATCCTGCGACGGTGGAAGAGGGAGGACTTGTTGAGGAGAGGGTCTCTGGCTCTGAGGGGACTTGCTCTGGTTTTTTCATTGCTGGCCTTCATCATCATGGCCAGCAACAAACACGGCTATGGGAAGAATTTCGATGAATATGAAGAATACAGGTATGTGTTGGCAATAGCGATTCTGGCGACGTTGTACACCGGGGCGCAAGCATTCCGGCATGCCCACGAGCTTTCCACCGGCACAGAAATGTTTCAGCAACGGACGTCGGCGTTGGCTGACTTTATCGGCGATCAGATTATGGCTTATCTACTGATATCATCGTCATCGTCAGCGATTCCATTGACGAATAGCATGAGAAATTATCAAGACAACCTATTCACAGACGCTGCAGCATCTGCCATTAGCATGGCCTTTCTTGCTTTCGTTGCTCTTGCAATATCAGCTCTAATTTCCGGTCACAAATTATCGACTCAATCTTACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

211

Amino Acids

22.94

Weight (kDa)

6.42

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 61 - 192 1.1e-28 Casparian strip membrane protein domain
MARVEL PF01284 63 - 198 1.6e-08 Membrane-associating domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012275)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38480
fragaria_vesca FvH4_6g10520
malus_domestica MD04G1167600.v1.1 MD12G1180900.v1.1
prunus_persica Prupe.6G285800_v2.0.a1 Prupe.6G285900_v2.0.a1
pyrus_communis pycom04g14880 pycom12g17050
rosa_chinensis RchiOBHm_Chr3g0461491
rosa_laevigata RLG00000024930
rosa_multiflora Rmu_sc0002677.1_g000004
rosa_roxburghii Rroxscaffold_6G00418240 Rroxscaffold_6G00418570
rosa_rugosa Rorug03G0051200.1
rosa_samantha Rh3AG108900 Rh3BG112600 Rh3CG114300 Rh3DG114400
rosa_wichuraiana Rw3G009260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 413
AccII CGCG 2 cut(s) 76, 149
AciI CCGC 3 cut(s) 56, 87, 149
AclWI GGATC 3 cut(s) 58, 148, 161
AcoI YGGCCR 2 cut(s) 84, 252
AcsI RAATTY 2 cut(s) 29, 280
AcyI GRCGYC 1 cut(s) 410
AfaI GTAC 1 cut(s) 341
AjiI CACGTC 1 cut(s) 94
AluBI AGCT 2 cut(s) 376, 590
AluI AGCT 2 cut(s) 376, 590
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 3 cut(s) 58, 148, 161
AlwNI CAGNNNCTG 2 cut(s) 533, 542
AoxI GGCC 4 cut(s) 84, 237, 252, 555
ApeKI GCWGC 2 cut(s) 533, 536
ApoI RAATTY 2 cut(s) 29, 280
Asp700I GAANNNNTTC 2 cut(s) 281, 396
AspLEI GCGC 2 cut(s) 78, 352
AsuC2I CCSGG 2 cut(s) 62, 346
AsuHPI GGTGA 1 cut(s) 45
BalI TGGCCA 1 cut(s) 254
BamHI GGATCC 1 cut(s) 153
BauI CACGAG 1 cut(s) 371
BbvI GCAGC 2 cut(s) 520, 548
BceAI ACGGC 1 cut(s) 283
BcnI CCSGG 2 cut(s) 62, 346
BcoDI GTCTC 1 cut(s) 201
BfmI CTRYAG 1 cut(s) 534
BisI GCNGC 3 cut(s) 87, 534, 537
BlsI GCNGC 3 cut(s) 88, 535, 538
Bme1390I CCNGG 2 cut(s) 62, 346
BmgBI CACGTC 1 cut(s) 94
BmiI GGNNCC 1 cut(s) 155
BmrFI CCNGG 2 cut(s) 62, 346
BmsI GCATC 2 cut(s) 7, 548
BplI GAGNNNNNCTC 2 cut(s) 181, 213
BpuMI CCSGG 2 cut(s) 62, 346
BsaHI GRCGYC 1 cut(s) 410
BsaI GGTCTC 1 cut(s) 201
BsaJI CCNNGG 2 cut(s) 60, 345
BsaWI WCCGGW 1 cut(s) 599
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bse118I RCCGGY 2 cut(s) 82, 383
Bse3DI GCAATG 1 cut(s) 230
BseDI CCNNGG 2 cut(s) 60, 345
BseMI GCAATG 1 cut(s) 230
BseMII CTCAG 1 cut(s) 197
BseRI GAGGAG 1 cut(s) 202
BseX3I CGGCCG 1 cut(s) 84
BseXI GCAGC 2 cut(s) 520, 548
BseYI CCCAGC 1 cut(s) 128
Bsh1236I CGCG 2 cut(s) 76, 149
Bsh1285I CGRYCG 1 cut(s) 87
BshFI GGCC 4 cut(s) 86, 239, 254, 557
BsiEI CGRYCG 1 cut(s) 87
BsiSI CCGG 6 cut(s) 62, 83, 345, 361, 384, 600
BslFI GGGAC 4 cut(s) 53, 97, 145, 225
BsmAI GTCTC 1 cut(s) 201
BsmFI GGGAC 4 cut(s) 53, 97, 145, 225
BsmI GAATGC 1 cut(s) 356
BsnI GGCC 4 cut(s) 86, 239, 254, 557
Bso31I GGTCTC 1 cut(s) 201
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 4 cut(s) 50, 117, 153, 436
BspACI CCGC 3 cut(s) 56, 87, 149
BspANI GGCC 4 cut(s) 86, 239, 254, 557
BspCNI CTCAG 1 cut(s) 198
BspFNI CGCG 2 cut(s) 76, 149
BspLI GGNNCC 1 cut(s) 155
BspMAI CTGCAG 1 cut(s) 538
BspPI GGATC 3 cut(s) 58, 148, 161
BspTNI GGTCTC 1 cut(s) 201
BsrDI GCAATG 1 cut(s) 230
BsrFI RCCGGY 2 cut(s) 82, 383
BsrGI TGTACA 1 cut(s) 339
BssAI RCCGGY 2 cut(s) 82, 383
BssECI CCNNGG 2 cut(s) 60, 345
BssMI GATC 4 cut(s) 50, 117, 153, 436
BssNI GRCGYC 1 cut(s) 410
BssSI CACGAG 1 cut(s) 371
Bst2BI CACGAG 1 cut(s) 371
Bst4CI ACNGT 1 cut(s) 165
Bst6I CTCTTC 1 cut(s) 164
BstACI GRCGYC 1 cut(s) 410
BstAPI GCANNNNNTGC 1 cut(s) 542
BstAUI TGTACA 1 cut(s) 339
BstC8I GCNNGC 4 cut(s) 237, 256, 354, 366
BstDEI CTNAG 1 cut(s) 206
BstFNI CGCG 2 cut(s) 76, 149
BstHHI GCGC 2 cut(s) 78, 352
BstKTI GATC 4 cut(s) 53, 120, 156, 439
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 4 cut(s) 50, 117, 153, 436
BstMCI CGRYCG 1 cut(s) 87
BstMWI GCNNNNNNNGC 5 cut(s) 329, 542, 563, 572, 587
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 2 cut(s) 60, 344
BstSFI CTRYAG 1 cut(s) 534
BstUI CGCG 2 cut(s) 76, 149
BstV1I GCAGC 2 cut(s) 520, 548
BstX2I RGATCY 1 cut(s) 153
BstXI CCANNNNNNTGG 1 cut(s) 553
BstYI RGATCY 1 cut(s) 153
BstZI CGGCCG 1 cut(s) 84
BsuRI GGCC 4 cut(s) 86, 239, 254, 557
BtrI CACGTC 1 cut(s) 94
Cac8I GCNNGC 4 cut(s) 237, 256, 354, 366
CaiI CAGNNNCTG 2 cut(s) 533, 542
CfoI GCGC 2 cut(s) 78, 352
Cfr10I RCCGGY 2 cut(s) 82, 383
CseI GACGC 1 cut(s) 539
Csp6I GTAC 1 cut(s) 340
CviAII CATG 4 cut(s) 250, 365, 499, 553
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 1 cut(s) 206
DpnI GATC 4 cut(s) 52, 119, 155, 438
DpnII GATC 4 cut(s) 50, 117, 153, 436
EaeI YGGCCR 2 cut(s) 84, 252
EagI CGGCCG 1 cut(s) 84
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EclXI CGGCCG 1 cut(s) 84
Eco31I GGTCTC 1 cut(s) 201
Eco32I GATATC 1 cut(s) 462
Eco52I CGGCCG 1 cut(s) 84
EcoRV GATATC 1 cut(s) 462
FaeI CATG 4 cut(s) 253, 368, 502, 556
FaiI YATR 8 cut(s) 251, 273, 294, 309, 366, 446, 500, 554
FaqI GGGAC 4 cut(s) 53, 97, 145, 225
FatI CATG 4 cut(s) 249, 364, 498, 552
FauI CCCGC 1 cut(s) 142
Fnu4HI GCNGC 3 cut(s) 87, 534, 537
Fsp4HI GCNGC 3 cut(s) 87, 534, 537
GlaI GCGC 2 cut(s) 77, 351
GluI GCNGC 3 cut(s) 87, 534, 537
GsaI CCCAGC 1 cut(s) 132
HaeIII GGCC 4 cut(s) 86, 239, 254, 557
HapII CCGG 6 cut(s) 62, 83, 345, 361, 384, 600
HgaI GACGC 1 cut(s) 539
HhaI GCGC 2 cut(s) 78, 352
Hin1I GRCGYC 1 cut(s) 410
Hin1II CATG 4 cut(s) 253, 368, 502, 556
Hin6I GCGC 2 cut(s) 76, 350
HinP1I GCGC 2 cut(s) 76, 350
HinfI GANTC 5 cut(s) 7, 100, 325, 481, 616
HpaII CCGG 6 cut(s) 62, 83, 345, 361, 384, 600
HphI GGTGA 1 cut(s) 45
Hpy166II GTNNAC 1 cut(s) 342
Hpy188I TCNGA 5 cut(s) 50, 105, 122, 207, 441
Hpy188III TCNNGA 2 cut(s) 11, 512
Hpy8I GTNNAC 1 cut(s) 342
Hpy99I CGWCG 3 cut(s) 165, 337, 415
HpyAV CCTTC 1 cut(s) 250
HpyCH4III ACNGT 1 cut(s) 165
HpyCH4IV ACGT 3 cut(s) 93, 335, 410
HpyCH4V TGCA 3 cut(s) 20, 536, 581
HpyF10VI GCNNNNNNNGC 5 cut(s) 329, 542, 563, 572, 587
HpyF3I CTNAG 1 cut(s) 206
HpySE526I ACGT 3 cut(s) 93, 335, 410
Hsp92I GRCGYC 1 cut(s) 410
Hsp92II CATG 4 cut(s) 253, 368, 502, 556
HspAI GCGC 2 cut(s) 76, 350
Kzo9I GATC 4 cut(s) 50, 117, 153, 436
Lsp1109I GCAGC 2 cut(s) 520, 548
LweI GCATC 2 cut(s) 7, 548
MaeII ACGT 3 cut(s) 93, 335, 410
MaeIII GTNAC 1 cut(s) 602
MalI GATC 4 cut(s) 52, 119, 155, 438
MboI GATC 4 cut(s) 50, 117, 153, 436
MboII GAAGA 4 cut(s) 16, 181, 289, 308
MflI RGATCY 1 cut(s) 153
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 5 cut(s) 29, 280, 505, 594, 608
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 1 cut(s) 610
MmeI TCCRAC 1 cut(s) 28
MnlI CCTC 6 cut(s) 37, 165, 169, 180, 185, 201
Mox20I TGGCCA 1 cut(s) 254
MroXI GAANNNNTTC 2 cut(s) 281, 396
MscI TGGCCA 1 cut(s) 254
MslI CAYNNNNRTG 4 cut(s) 248, 270, 392, 551
Msp20I TGGCCA 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 6 cut(s) 62, 83, 345, 361, 384, 600
MspR9I CCNGG 2 cut(s) 62, 346
Mva1269I GAATGC 1 cut(s) 356
MvnI CGCG 2 cut(s) 76, 149
MwoI GCNNNNNNNGC 5 cut(s) 329, 542, 563, 572, 587
NciI CCSGG 2 cut(s) 62, 346
NdeII GATC 4 cut(s) 50, 117, 153, 436
NlaIII CATG 4 cut(s) 253, 368, 502, 556
NlaIV GGNNCC 1 cut(s) 155
NmuCI GTSAC 1 cut(s) 602
NspI RCATGY 1 cut(s) 368
PaeI GCATGC 1 cut(s) 368
PctI GAATGC 1 cut(s) 356
PdmI GAANNNNTTC 2 cut(s) 281, 396
PfeI GAWTC 4 cut(s) 7, 100, 325, 481
PflFI GACNNNGTC 1 cut(s) 109
PkrI GCNGC 3 cut(s) 88, 535, 538
PleI GAGTC 1 cut(s) 610
PpsI GAGTC 1 cut(s) 610
PspFI CCCAGC 1 cut(s) 128
PspN4I GGNNCC 1 cut(s) 155
PstI CTGCAG 1 cut(s) 538
PstNI CAGNNNCTG 2 cut(s) 533, 542
PsuI RGATCY 1 cut(s) 153
PsyI GACNNNGTC 1 cut(s) 109
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
RseI CAYNNNNRTG 4 cut(s) 248, 270, 392, 551
SatI GCNGC 3 cut(s) 87, 534, 537
Sau3AI GATC 4 cut(s) 50, 117, 153, 436
SchI GAGTC 1 cut(s) 610
ScrFI CCNGG 2 cut(s) 62, 346
SetI ASST 8 cut(s) 48, 96, 308, 338, 378, 413, 522, 592
SfaNI GCATC 2 cut(s) 7, 548
SfcI CTRYAG 1 cut(s) 534
SmiMI CAYNNNNRTG 4 cut(s) 248, 270, 392, 551
SphI GCATGC 1 cut(s) 368
Sse9I AATT 5 cut(s) 29, 280, 505, 594, 608
SsiI CCGC 3 cut(s) 56, 87, 149
StyD4I CCNGG 2 cut(s) 60, 344
TaaI ACNGT 1 cut(s) 165
TaiI ACGT 3 cut(s) 96, 338, 413
TaqI TCGA 3 cut(s) 33, 285, 614
TasI AATT 5 cut(s) 29, 280, 505, 594, 608
TatI WGTACW 1 cut(s) 339
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 4 cut(s) 7, 100, 325, 481
TseFI GTSAC 1 cut(s) 602
TseI GCWGC 2 cut(s) 533, 536
Tsp45I GTSAC 1 cut(s) 602
TspDTI ATGAA 6 cut(s) 17, 51, 219, 232, 303, 309
TspGWI ACGGA 1 cut(s) 421
Tth111I GACNNNGTC 1 cut(s) 109
XapI RAATTY 2 cut(s) 29, 280
XceI RCATGY 1 cut(s) 368
XmnI GAANNNNTTC 2 cut(s) 281, 396
ZraI GACGTC 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.